Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0002222

Physical Location & Seq

Genomic Coordinates Reverse (-)

4253960 .. 4258835

Gene Structure 4876 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G004480.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2973 bp

ATGACCACTCCTCTGACCGCCACGTCATCACTATCGTTCTTCTTCATCATCATCGTCTCCATCGCCGTAGCCGTCTCCATCTCTCTCTTCCATTACAAGCCCCACCAGAACCCCGCAACCCTAGATCCTCCTCCACCGCAAAACTACGTCGTTCGATTCGTCGAGTACAAGAGGTGGGAGGATCACCGGGAGTATCTGAAATCGAGTGTGCCGGCGGAGGGATGGGACTGGATCGACAGGAACAATCCGGCCATGGACTACCCGACGGATTTCGGGTTGGTGCGGATTGACGATGGAGCCAGGGAATTGGTGATTGGGGAGATTGGGAAATTGGGGATGGTGAAGGATGTGAATGCTGACGTCAGGTATGGGAGGGGTTTGCTTAGTGAGGAGGAGAAGAGGGAGAAAGTTGGGGCCTTTGTGGATGGGGAGAAGCGGCCGGGGAAGATGTTCACGGCGATGTCGTATAGCGAAGACGACGGAGCAGTTAGTAATGGCTCTGTTAGCTGGAAAAGGGAGCTCTTGGCTGAGAGATCTCAAATAACTTCATTATTTGGAGCGGATTTTCTTTGGACAAAAGGGTATACCGGTACGAAAGTGAAAATGGCCATATTTGATACTGGTATTCGAGCTAATCACCCGCACTTTCGAAATATTAAGGAGCGGACAAACTGGACAAATGAGGACACATTAAACGACAATCTTGGACATGGGACATTTGTTGCTGGTGTTGTTGCTGGTGTAGATGCAGAGTGTCTTGGTTTTGCTCCTGATACAGAGATATATGCGTTTCGTGTGTTTACAGATGCACAGGTATCATACACATCATGGTTCCTTGATGCATTCAACTATGCCATTGCGACCAATATGGATGTACTGAACTTGAGCATAGGTGGACCTGATTACTTGGACCTCCCATTTGTGGAGAAGGTCTGGGAAATAACAGCAAACAACATAATTATGGTTTCAGCAATTGGAAACGATGGACCTGATGTCATTGGTGTTGGTGGCATCGACTACAGTGATCACATTGCTTCTTTTTCCTCACGAGGCATGAGTACTTGGGAGATGCCTCACGGTTACGGTCGTGTTAAGCCAGATGTCGTTGCATATGGACGGGAAATTATGGGATCCAAGATTAGTACAGGCTGTAAAAGCTTATCTGGCACTAGTGTGGCAAGTCCTGTGGTTGCTGGTATGGTTTGTCTGCTTGTGAGTGTTATCCCGGAAAGCAGTCGGAAGGATACTTTAAATCCAGCAAGCATGAAACAAGCATTGGTTGAGGGTGCTGCAAAACTCGTTGGTCCAAATATGTATGAGCAGGGTGCGGGGAGGGTTGATCTGTTAGAATCATATGAAATCCTGAAGAATTACCAACCTCGTGCCAGCATCTTCCCTAGTATTCTTGATTATACAGACTGCCCATACTCTTGGCCCTTCTGTCGTCAGCCACTTTATGCTGGTGCCATGCCTGTTATCTTCAATGCTACCATTCTAAATGGAATGGGTGTAATTGGCTATGTTGAAAGTCCACCAACTTGGCATCCTTCAGATGAAGTAGGGAATCTTCTAAGTATTCACTTTACATATTCTAATGTTATCTGGCCTTGGACTGGTTATTTAGCGCTTCACATGCAAATTAAGGACGAAGGTGCACAATTTTCAGGAGAGATTAGTGGCAATGTTACTCTTAGGGTATACAGTCCTCCATCTCCGGGAGAAAAGAATCTTCGGATTAGCACTTGTGTGCTTCAGTTAAGATTGAAGATTGTTCCAACTCCACCAAGATCAAAACGAGTTTTGTGGGACCAGTTTCACAGTATTAAATACCCTCCAGGTTACATCCCTAGAGACTCTTTGGATGTTCGCAATGACATTCTTGACTGGCATGGAGATCACCTATGCTGGGTATTTGTAGAAACACTTGGTTCACCTCTTACTTGCTTTGATGCTCTTCGATATGGGACACTTCTTCTGGTAGATCTTGAAGAAGAGTACTTTCAGGAGGAGATTGAGAAGCTGCGAGATGATGTTCTTAATTCTGGACTGGGATTGGTTGTGTTTTCTGAATTCTTTGATGATAACACACGTAGCTGGTGGACTCCAGTCACTGGAGGTGCAAATGTTCCAGCGTTGAACGACCTGCTGGCTCCGTTTGGGATTGCATTTGGAGATAAGATTCTGAATGGTGATTTTTCTATCAATGGTGAGCAAAGTCGGTATGCATCTGGAACGGATATTGTGAGGTTTCCGAAAGGGGGTTATGTACACAAGTTCCCTTTCCTGGATAGCTCTGAAAGTGGGGCCACTCAGAATGTACTTCGGGATCCTGAGGTGACAATGGCAGATTCTCCCATTCTTGGTCTTGTACAGGTGGGTGAAGGTCGTGTTGTAGTGTATGGAGATTCTAACTGTTTGGACAGCAGTCATATGGTAACTAATTGTTATTGGCTCTTGAGAAAAATGTTAGATTTCACCGGTGGAAACATCAGAGATCCTGTGCTTTTTTCAAGCTCGGTCAAACAAAGTTCACCTTTATATATGGAAGATAACCAATTACCGTCTCGTAGAACAGATGTGAATTTTTCTACATATTCCGCTGTAGTGGGGAAGGAATTGATCTGTGGGAGTGACTCTGTATTTGAAATATGGGGGACTAAAGGTTACAGTTTACAGGTCAAGGGAACAAACAGAAGATTACCAGGCTCTTCTGTGTCTAATCCAGGTAGAGGTTTGAATTCTACTGTCGAGGCTTCCCATTTGGAGCATTCCAAGTTGACACTGGGAAATAGAAGTGATCCTTCGGGATATAGGGATGAGCTTGATATGCCTGTGTTAGTTGCTAGTCACTGGCTTGTTCCTGCAATAATTGCCGTAACTGGTTTGGTGGTATTTTTGAGCTTCTGGAGAATTAGACAGAAGCGACGACGACGAAGAAAAGGATCTAGCTCTGGTCGATATTCTAATTTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0004252 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005788 GO:0005789 GO:0005794 GO:0006464 GO:0006508 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0006984 GO:0006986 GO:0006996 GO:0007033 GO:0007040 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008237 GO:0009628 GO:0009651 GO:0009889 GO:0009987 GO:0010033 GO:0012505 GO:0016020 GO:0016043 GO:0016787 GO:0017171 GO:0019216 GO:0019218 GO:0019222 GO:0019538 GO:0023052 GO:0030968 GO:0031090 GO:0031293 GO:0031974 GO:0031984 GO:0033554 GO:0033619 GO:0034620 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036500 GO:0042175 GO:0042221 GO:0042538 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043687 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045540 GO:0046890 GO:0050789 GO:0050794 GO:0050810 GO:0050896 GO:0051716 GO:0062012 GO:0065007 GO:0070011 GO:0070013 GO:0070887 GO:0071310 GO:0071704 GO:0071840 GO:0080090 GO:0080171 GO:0090181 GO:0098588 GO:0098791 GO:0098827 GO:0106118 GO:0140096 GO:1901564 GO:1902930

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Peptidase_S8

Protein Analysis

990

Amino Acids

109.8

Weight (kDa)

5.48

Isoelectric Point (pI)

41.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MBTP1_N	PF23001	47 - 119	5.8e-13	Membrane-bound Site-1 Protease Family N-terminal domain
Peptidase_S8	PF00082	197 - 437	2e-36	Subtilase family
MBTPS1_3rd	PF23094	465 - 593	2.5e-54	MBTPS1, third Ig-like domain
MBTPS1_4th	PF23090	594 - 841	4.5e-114	MBTPS1, fourth GATase-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002222)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19660 AT5G19660 AT5G19660
fragaria_vesca	FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670
malus_domestica	MD02G1045500.v1.1 MD15G1183900.v1.1
prunus_persica	Prupe.7G232300_v2.0.a1 Prupe.7G232300_v2.0.a1
pyrus_communis	pycom02g03790 pycom02g03800 pycom02g03810 pycom15g16550
rosa_chinensis	RchiOBHm_Chr2g0090281
rosa_laevigata	RLG00000008526 RLG00000016094 RLG00000021848 RLG00000032088
rosa_multiflora	Rmu_sc0000714.1_g000011 Rmu_sc0004834.1_g000013 Rmu_sc0005276.1_g000005
rosa_roxburghii	Rroxscaffold_2G00085240 Rroxscaffold_2G00151320
rosa_rugosa	Rorug02G0006200
rosa_samantha	Rh1DG037900 Rh2AG052100 Rh2BG050700 Rh2CG052900 Rh2DG052000 Rh5AG367600 Rh5AG473400 Rh5BG377700 Rh5BG493700 Rh5CG401100 Rh5CG517700 Rh5DG391000 Rh5DG505700
rosa_wichuraiana	Rw2G004480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	22
AatII	GACGTC	1 cut(s)	363
Acc36I	ACCTGC	1 cut(s)	2151
AccB1I	GGYRCC	1 cut(s)	1463
AccB7I	CCANNNNNTGG	1 cut(s)	2111
AccBSI	CCGCTC	2 cut(s)	560, 664
AccI	GTMKAC	2 cut(s)	584, 1698
AcoI	YGGCCR	3 cut(s)	249, 437, 606
AcsI	RAATTY	3 cut(s)	2069, 2581, 2737
AcuI	CTGAAG	3 cut(s)	1385, 1533, 1736
AcyI	GRCGYC	1 cut(s)	360
AfaI	GTAC	9 cut(s)	167, 592, 876, 1060, 1144, 1997, 2268, 2319, 2370
AfeI	AGCGCT	1 cut(s)	1626
AflIII	ACRYGT	1 cut(s)	2087
AgeI	ACCGGT	2 cut(s)	587, 2477
AgsI	TTSAA	9 cut(s)	847, 1483, 1526, 1765, 1988, 2137, 2511, 2645, 2737
AhdI	GACNNNNNGTC	1 cut(s)	992
AhlI	ACTAGT	1 cut(s)	1169
AjiI	CACGTC	1 cut(s)	24
AjnI	CCWGG	5 cut(s)	299, 1834, 2283, 2701, 2722
AjuI	GAANNNNNNNTTGG	4 cut(s)	297, 329, 1259, 1291
AleI	CACNNNNGTG	2 cut(s)	1172, 1745
Alw21I	GWGCWC	2 cut(s)	522, 1657
Alw26I	GTCTC	4 cut(s)	61, 79, 1845, 2568
Alw44I	GTGCAC	1 cut(s)	1653
AlwNI	CAGNNNCTG	1 cut(s)	2111
Aor51HI	AGCGCT	1 cut(s)	1626
AoxI	GGCC	7 cut(s)	249, 414, 437, 606, 1433, 1604, 2304
ApaLI	GTGCAC	1 cut(s)	1653
ApeKI	GCWGC	2 cut(s)	1289, 2020
ApoI	RAATTY	3 cut(s)	2069, 2581, 2737
ArsI	GACNNNNNNTTYG	4 cut(s)	902, 934, 2624, 2656
AsiGI	ACCGGT	2 cut(s)	587, 2477
Asp700I	GAANNNNTTC	1 cut(s)	449
AspLEI	GCGC	1 cut(s)	1627
AspS9I	GGNCC	8 cut(s)	414, 896, 910, 986, 1304, 1434, 1807, 2304
AsuC2I	CCSGG	4 cut(s)	188, 441, 1226, 1716
AsuII	TTCGAA	1 cut(s)	649
AvaII	GGWCC	5 cut(s)	896, 910, 986, 1304, 1807
AxyI	CCTNAGG	1 cut(s)	2331
BaeGI	GKGCMC	1 cut(s)	1657
BaeI	ACNNNNGTAYC	2 cut(s)	582, 615
BalI	TGGCCA	1 cut(s)	608
BamHI	GGATCC	2 cut(s)	1130, 2326
BanI	GGYRCC	1 cut(s)	1463
BanII	GRGCYC	1 cut(s)	522
BarI	GAAGNNNNNNTAC	2 cut(s)	1551, 1583
BauI	CACGAG	2 cut(s)	1047, 1380
BbsI	GAAGAC	1 cut(s)	480
Bbv12I	GWGCWC	2 cut(s)	522, 1657
BbvI	GCAGC	2 cut(s)	1276, 2007
BccI	CCATC	8 cut(s)	68, 86, 216, 287, 331, 419, 977, 1717
BceAI	ACGGC	4 cut(s)	50, 56, 471, 2858
BciT130I	CCWGG	5 cut(s)	301, 1836, 2285, 2703, 2724
BciVI	GTATCC	1 cut(s)	1237
BclI	TGATCA	1 cut(s)	1024
BcnI	CCSGG	4 cut(s)	188, 441, 1226, 1716
BcoDI	GTCTC	4 cut(s)	61, 79, 1845, 2568
BcuI	ACTAGT	1 cut(s)	1169
BfaI	CTAG	6 cut(s)	122, 1170, 1398, 1848, 2844, 2946
BfmI	CTRYAG	2 cut(s)	1018, 2601
BfoI	RGCGCY	1 cut(s)	1628
BfuAI	ACCTGC	1 cut(s)	2151
BfuI	GTATCC	1 cut(s)	1237
BglII	AGATCT	2 cut(s)	533, 1981
BisI	GCNGC	3 cut(s)	437, 1290, 2021
BlsI	GCNGC	3 cut(s)	438, 1291, 2022
BmcAI	AGTACT	2 cut(s)	1060, 1997
Bme1390I	CCNGG	9 cut(s)	188, 301, 441, 1226, 1716, 1836, 2285, 2703, 2724
Bme18I	GGWCC	5 cut(s)	896, 910, 986, 1304, 1807
BmeRI	GACNNNNNGTC	1 cut(s)	992
BmgBI	CACGTC	1 cut(s)	24
BmgT120I	GGNCC	8 cut(s)	414, 896, 910, 986, 1304, 1434, 1807, 2304
BmiI	GGNNCC	9 cut(s)	298, 415, 833, 1132, 1465, 1808, 2151, 2305, 2328
BmrFI	CCNGG	9 cut(s)	188, 301, 441, 1226, 1716, 1836, 2285, 2703, 2724
BmrI	ACTGGG	2 cut(s)	2057, 2792
BmsI	GCATC	9 cut(s)	736, 796, 829, 1020, 1059, 1398, 1552, 1939, 2234
BmuI	ACTGGG	2 cut(s)	2057, 2792
BoxI	GACNNNNGTC	2 cut(s)	2105, 2424
BpiI	GAAGAC	1 cut(s)	480
BpmI	CTGGAG	4 cut(s)	1818, 2088, 2133, 2926
Bpu14I	TTCGAA	1 cut(s)	649
BpuEI	CTTGAG	2 cut(s)	904, 2476
BpuMI	CCSGG	4 cut(s)	188, 441, 1226, 1716
BsaAI	YACGTR	1 cut(s)	2090
BsaHI	GRCGYC	1 cut(s)	360
BsaJI	CCNNGG	4 cut(s)	252, 300, 440, 1607
BsaWI	WCCGGW	2 cut(s)	587, 2477
Bse118I	RCCGGY	3 cut(s)	211, 587, 2477
Bse21I	CCTNAGG	1 cut(s)	2331
Bse3DI	GCAATG	4 cut(s)	855, 1029, 1687, 1876
BseBI	CCWGG	5 cut(s)	301, 1836, 2285, 2703, 2724
BseDI	CCNNGG	4 cut(s)	252, 300, 440, 1607
BseGI	GGATG	9 cut(s)	227, 342, 352, 430, 877, 1543, 1842, 1867, 2821
BseMI	GCAATG	4 cut(s)	855, 1029, 1687, 1876
BseMII	CTCAG	3 cut(s)	519, 2322, 2324
BseRI	GAGGAG	4 cut(s)	120, 404, 407, 2021
BseSI	GKGCMC	1 cut(s)	1657
BseX3I	CGGCCG	1 cut(s)	437
BseXI	GCAGC	2 cut(s)	1276, 2007
BseYI	CCCAGC	1 cut(s)	1905
Bsh1285I	CGRYCG	2 cut(s)	440, 1087
BshFI	GGCC	7 cut(s)	251, 416, 439, 608, 1435, 1606, 2306
BshNI	GGYRCC	1 cut(s)	1463
BshTI	ACCGGT	2 cut(s)	587, 2477
BsiEI	CGRYCG	2 cut(s)	440, 1087
BsiHKAI	GWGCWC	2 cut(s)	522, 1657
BsiSI	CCGG	8 cut(s)	187, 212, 248, 440, 588, 1226, 1715, 2478
BslFI	GGGAC	5 cut(s)	239, 727, 1820, 1978, 2668
BsmAI	GTCTC	4 cut(s)	61, 79, 1845, 2568
BsmBI	CGTCTC	3 cut(s)	61, 79, 2568
BsmFI	GGGAC	5 cut(s)	239, 727, 1820, 1978, 2668
BsmI	GAATGC	3 cut(s)	358, 842, 2767
BsnI	GGCC	7 cut(s)	251, 416, 439, 608, 1435, 1606, 2306
Bsp119I	TTCGAA	1 cut(s)	649
Bsp1286I	GDGCHC	2 cut(s)	522, 1657
Bsp1407I	TGTACA	2 cut(s)	2266, 2368
Bsp19I	CCATGG	1 cut(s)	252
BspANI	GGCC	7 cut(s)	251, 416, 439, 608, 1435, 1606, 2306
BspCNI	CTCAG	3 cut(s)	520, 2323, 2323
BspLI	GGNNCC	9 cut(s)	298, 415, 833, 1132, 1465, 1808, 2151, 2305, 2328
BspMI	ACCTGC	1 cut(s)	2151
BspQI	GCTCTTC	2 cut(s)	1959, 2713
BspT104I	TTCGAA	1 cut(s)	649
BspT107I	GGYRCC	1 cut(s)	1463
BsrBI	CCGCTC	2 cut(s)	560, 664
BsrDI	GCAATG	4 cut(s)	855, 1029, 1687, 1876
BsrFI	RCCGGY	3 cut(s)	211, 587, 2477
BsrGI	TGTACA	2 cut(s)	2266, 2368
BssAI	RCCGGY	3 cut(s)	211, 587, 2477
BssECI	CCNNGG	4 cut(s)	252, 300, 440, 1607
BssNAI	GTATAC	2 cut(s)	585, 1699
BssNI	GRCGYC	1 cut(s)	360
BssSI	CACGAG	2 cut(s)	1047, 1380
BssT1I	CCWWGG	2 cut(s)	252, 1607
Bst1107I	GTATAC	2 cut(s)	585, 1699
Bst2BI	CACGAG	2 cut(s)	1047, 1380
Bst2UI	CCWGG	5 cut(s)	301, 1836, 2285, 2703, 2724
Bst4CI	ACNGT	9 cut(s)	1022, 1079, 1085, 1703, 1820, 2414, 2562, 2669, 2746
Bst6I	CTCTTC	5 cut(s)	92, 392, 1959, 1986, 2713
BstACI	GRCGYC	1 cut(s)	360
BstAPI	GCANNNNNTGC	1 cut(s)	2870
BstAUI	TGTACA	2 cut(s)	2266, 2368
BstBAI	YACGTR	1 cut(s)	2090
BstBI	TTCGAA	1 cut(s)	649
BstC8I	GCNNGC	4 cut(s)	213, 1261, 1387, 2148
BstDEI	CTNAG	6 cut(s)	383, 528, 1571, 1691, 2310, 2331
BstDSI	CCRYGG	1 cut(s)	252
BstF5I	GGATG	9 cut(s)	227, 342, 352, 430, 877, 1543, 1842, 1867, 2821
BstH2I	RGCGCY	1 cut(s)	1628
BstHHI	GCGC	1 cut(s)	1627
BstMAI	GTCTC	4 cut(s)	61, 79, 1845, 2568
BstMCI	CGRYCG	2 cut(s)	440, 1087
BstMWI	GCNNNNNNNGC	6 cut(s)	504, 1155, 1164, 1633, 2827, 2870
BstNI	CCWGG	5 cut(s)	301, 1836, 2285, 2703, 2724
BstNSI	RCATGY	1 cut(s)	1636
BstPAI	GACNNNNGTC	2 cut(s)	2105, 2424
BstSCI	CCNGG	9 cut(s)	186, 299, 439, 1224, 1714, 1834, 2283, 2701, 2722
BstSFI	CTRYAG	2 cut(s)	1018, 2601
BstSLI	GKGCMC	1 cut(s)	1657
BstV1I	GCAGC	2 cut(s)	1276, 2007
BstV2I	GAAGAC	1 cut(s)	480
BstX2I	RGATCY	7 cut(s)	124, 533, 1130, 1981, 2326, 2494, 2942
BstXI	CCANNNNNNTGG	3 cut(s)	307, 1431, 1539
BstYI	RGATCY	7 cut(s)	124, 533, 1130, 1981, 2326, 2494, 2942
BstZ17I	GTATAC	2 cut(s)	585, 1699
BstZI	CGGCCG	1 cut(s)	437
Bsu36I	CCTNAGG	1 cut(s)	2331
BsuI	GTATCC	1 cut(s)	1237
BsuRI	GGCC	7 cut(s)	251, 416, 439, 608, 1435, 1606, 2306
BtgI	CCRYGG	1 cut(s)	252
BtgZI	GCGATG	2 cut(s)	46, 473
BtrI	CACGTC	1 cut(s)	24
BtsCI	GGATG	9 cut(s)	227, 342, 352, 430, 877, 1543, 1842, 1867, 2821
BtsIMutI	CAGTG	4 cut(s)	1027, 2109, 2780, 2848
BveI	ACCTGC	1 cut(s)	2151
Cac8I	GCNNGC	4 cut(s)	213, 1261, 1387, 2148
CaiI	CAGNNNCTG	1 cut(s)	2111
CfoI	GCGC	1 cut(s)	1627
Cfr10I	RCCGGY	3 cut(s)	211, 587, 2477
Cfr13I	GGNCC	8 cut(s)	414, 896, 910, 986, 1304, 1434, 1807, 2304
Csp6I	GTAC	9 cut(s)	166, 591, 875, 1059, 1143, 1996, 2267, 2318, 2369
CspAI	ACCGGT	2 cut(s)	587, 2477
CspCI	CAANNNNNGTGG	2 cut(s)	1167, 1202
CviAII	CATG	8 cut(s)	253, 710, 828, 1054, 1264, 1468, 1633, 1889
CviQI	GTAC	9 cut(s)	166, 591, 875, 1059, 1143, 1996, 2267, 2318, 2369
DdeI	CTNAG	6 cut(s)	383, 528, 1571, 1691, 2310, 2331
DraI	TTTAAA	1 cut(s)	1251
DrdI	GACNNNNNNGTC	1 cut(s)	22
DriI	GACNNNNNGTC	1 cut(s)	992
DseDI	GACNNNNNNGTC	1 cut(s)	22
EaeI	YGGCCR	3 cut(s)	249, 437, 606
EagI	CGGCCG	1 cut(s)	437
Eam1104I	CTCTTC	5 cut(s)	92, 392, 1959, 1986, 2713
Eam1105I	GACNNNNNGTC	1 cut(s)	992
EarI	CTCTTC	5 cut(s)	92, 392, 1959, 1986, 2713
EciI	GGCGGA	1 cut(s)	230
Ecl136II	GAGCTC	1 cut(s)	520
EclXI	CGGCCG	1 cut(s)	437
Eco130I	CCWWGG	2 cut(s)	252, 1607
Eco24I	GRGCYC	1 cut(s)	522
Eco47I	GGWCC	5 cut(s)	896, 910, 986, 1304, 1807
Eco47III	AGCGCT	1 cut(s)	1626
Eco52I	CGGCCG	1 cut(s)	437
Eco53kI	GAGCTC	1 cut(s)	520
Eco57I	CTGAAG	3 cut(s)	1385, 1533, 1736
Eco81I	CCTNAGG	1 cut(s)	2331
EcoICRI	GAGCTC	1 cut(s)	520
EcoO109I	RGGNCCY	1 cut(s)	414
EcoRI	GAATTC	2 cut(s)	2069, 2737
EcoRII	CCWGG	5 cut(s)	299, 1834, 2283, 2701, 2722
EcoT14I	CCWWGG	2 cut(s)	252, 1607
EcoT22I	ATGCAT	2 cut(s)	844, 2227
EcoT38I	GRGCYC	1 cut(s)	522
ErhI	CCWWGG	2 cut(s)	252, 1607
Esp3I	CGTCTC	3 cut(s)	61, 79, 2568
FaeI	CATG	8 cut(s)	256, 713, 831, 1057, 1267, 1471, 1636, 1892
FalI	AAGNNNNNCTT	4 cut(s)	2518, 2550, 2785, 2817
FaqI	GGGAC	5 cut(s)	239, 727, 1820, 1978, 2668
FatI	CATG	8 cut(s)	252, 709, 827, 1053, 1263, 1467, 1632, 1888
FauI	CCCGC	3 cut(s)	121, 648, 1321
FauNDI	CATATG	3 cut(s)	1111, 1354, 2430
FbaI	TGATCA	1 cut(s)	1024
FblI	GTMKAC	2 cut(s)	584, 1698
Fnu4HI	GCNGC	3 cut(s)	437, 1290, 2021
FokI	GGATG	9 cut(s)	234, 349, 359, 437, 884, 1530, 1829, 1874, 2828
FriOI	GRGCYC	1 cut(s)	522
Fsp4HI	GCNGC	3 cut(s)	437, 1290, 2021
FspBI	CTAG	6 cut(s)	122, 1170, 1398, 1848, 2844, 2946
GlaI	GCGC	1 cut(s)	1626
GluI	GCNGC	3 cut(s)	437, 1290, 2021
GsaI	CCCAGC	1 cut(s)	1909
GsuI	CTGGAG	4 cut(s)	1818, 2088, 2133, 2926
HaeII	RGCGCY	1 cut(s)	1628
HaeIII	GGCC	7 cut(s)	251, 416, 439, 608, 1435, 1606, 2306
HapII	CCGG	8 cut(s)	187, 212, 248, 440, 588, 1226, 1715, 2478
HhaI	GCGC	1 cut(s)	1627
Hin1I	GRCGYC	1 cut(s)	360
Hin1II	CATG	8 cut(s)	256, 713, 831, 1057, 1267, 1471, 1636, 1892
Hin6I	GCGC	1 cut(s)	1625
HinP1I	GCGC	1 cut(s)	1625
HincII	GTYRAC	1 cut(s)	2778
HindII	GTYRAC	1 cut(s)	2778
HindIII	AAGCTT	1 cut(s)	1156
HpaII	CCGG	8 cut(s)	187, 212, 248, 440, 588, 1226, 1715, 2478
Hpy99I	CGWCG	7 cut(s)	152, 164, 268, 482, 2928, 2931, 2934
HpyAV	CCTTC	9 cut(s)	337, 922, 1234, 1447, 1557, 1643, 2375, 2605, 2811
HpyCH4III	ACNGT	9 cut(s)	1022, 1079, 1085, 1703, 1820, 2414, 2562, 2669, 2746
HpyCH4IV	ACGT	4 cut(s)	23, 147, 360, 2089
HpyF10VI	GCNNNNNNNGC	6 cut(s)	504, 1155, 1164, 1633, 2827, 2870
HpyF3I	CTNAG	6 cut(s)	383, 528, 1571, 1691, 2310, 2331
HpySE526I	ACGT	4 cut(s)	23, 147, 360, 2089
Hsp92I	GRCGYC	1 cut(s)	360
Hsp92II	CATG	8 cut(s)	256, 713, 831, 1057, 1267, 1471, 1636, 1892
HspAI	GCGC	1 cut(s)	1625
KroI	GCCGGC	1 cut(s)	211
KroNI	GCCGGC	1 cut(s)	213
Ksp22I	TGATCA	1 cut(s)	1024
LguI	GCTCTTC	2 cut(s)	1959, 2713
LmnI	GCTCC	8 cut(s)	296, 482, 517, 557, 661, 772, 2155, 2764
Lsp1109I	GCAGC	2 cut(s)	1276, 2007
LweI	GCATC	9 cut(s)	736, 796, 829, 1020, 1059, 1398, 1552, 1939, 2234
MaeI	CTAG	6 cut(s)	122, 1170, 1398, 1848, 2844, 2946
MaeII	ACGT	4 cut(s)	23, 147, 360, 2089
MbiI	CCGCTC	2 cut(s)	560, 664
MfeI	CAATTG	1 cut(s)	972
MflI	RGATCY	7 cut(s)	124, 533, 1130, 1981, 2326, 2494, 2942
MhlI	GDGCHC	2 cut(s)	522, 1657
MlsI	TGGCCA	1 cut(s)	608
MluNI	TGGCCA	1 cut(s)	608
MlyI	GAGTC	3 cut(s)	1847, 2095, 2627
MmeI	TCCRAC	2 cut(s)	1217, 1799
Mox20I	TGGCCA	1 cut(s)	608
Mph1103I	ATGCAT	2 cut(s)	844, 2227
MroNI	GCCGGC	1 cut(s)	211
MroXI	GAANNNNTTC	1 cut(s)	449
MscI	TGGCCA	1 cut(s)	608
MseI	TTAA	8 cut(s)	657, 692, 1092, 1250, 1641, 1757, 1824, 2037
MslI	CAYNNNNRTG	4 cut(s)	458, 959, 1172, 1745
Msp20I	TGGCCA	1 cut(s)	608
MspA1I	CMGCKG	1 cut(s)	2600
MspI	CCGG	8 cut(s)	187, 212, 248, 440, 588, 1226, 1715, 2478
MspR9I	CCNGG	9 cut(s)	188, 301, 441, 1226, 1716, 1836, 2285, 2703, 2724
MunI	CAATTG	1 cut(s)	972
Mva1269I	GAATGC	3 cut(s)	358, 842, 2767
MvaI	CCWGG	5 cut(s)	301, 1836, 2285, 2703, 2724
MwoI	GCNNNNNNNGC	6 cut(s)	504, 1155, 1164, 1633, 2827, 2870
NaeI	GCCGGC	1 cut(s)	213
NciI	CCSGG	4 cut(s)	188, 441, 1226, 1716
NcoI	CCATGG	1 cut(s)	252
NdeI	CATATG	3 cut(s)	1111, 1354, 2430
NgoMIV	GCCGGC	1 cut(s)	211
NlaIII	CATG	8 cut(s)	256, 713, 831, 1057, 1267, 1471, 1636, 1892
NlaIV	GGNNCC	9 cut(s)	298, 415, 833, 1132, 1465, 1808, 2151, 2305, 2328
NmuCI	GTSAC	4 cut(s)	2107, 2335, 2630, 2846
NsiI	ATGCAT	2 cut(s)	844, 2227
NspI	RCATGY	1 cut(s)	1636
NspV	TTCGAA	1 cut(s)	649
OliI	CACNNNNGTG	2 cut(s)	1172, 1745
PciSI	GCTCTTC	2 cut(s)	1959, 2713
PcsI	WCGNNNNNNNCGW	3 cut(s)	69, 156, 461
PctI	GAATGC	3 cut(s)	358, 842, 2767
PdiI	GCCGGC	1 cut(s)	213
PdmI	GAANNNNTTC	1 cut(s)	449
PfeI	GAWTC	7 cut(s)	156, 1349, 1564, 1726, 2179, 2348, 2405
PflMI	CCANNNNNTGG	1 cut(s)	2111
PfoI	TCCNGGA	3 cut(s)	1224, 1714, 2283
PinAI	ACCGGT	2 cut(s)	587, 2477
PkrI	GCNGC	3 cut(s)	438, 1291, 2022
PleI	GAGTC	3 cut(s)	1847, 2095, 2627
PpsI	GAGTC	3 cut(s)	1847, 2095, 2627
Ppu21I	YACGTR	1 cut(s)	2090
PshAI	GACNNNNGTC	2 cut(s)	2105, 2424
Psp124BI	GAGCTC	1 cut(s)	522
Psp6I	CCWGG	5 cut(s)	299, 1834, 2283, 2701, 2722
PspFI	CCCAGC	1 cut(s)	1905
PspGI	CCWGG	5 cut(s)	299, 1834, 2283, 2701, 2722
PspN4I	GGNNCC	9 cut(s)	298, 415, 833, 1132, 1465, 1808, 2151, 2305, 2328
PspPI	GGNCC	8 cut(s)	414, 896, 910, 986, 1304, 1434, 1807, 2304
PstNI	CAGNNNCTG	1 cut(s)	2111
PsuI	RGATCY	7 cut(s)	124, 533, 1130, 1981, 2326, 2494, 2942
RsaI	GTAC	9 cut(s)	167, 592, 876, 1060, 1144, 1997, 2268, 2319, 2370
RsaNI	GTAC	9 cut(s)	166, 591, 875, 1059, 1143, 1996, 2267, 2318, 2369
RseI	CAYNNNNRTG	4 cut(s)	458, 959, 1172, 1745
SacI	GAGCTC	1 cut(s)	522
SapI	GCTCTTC	2 cut(s)	1959, 2713
SaqAI	TTAA	8 cut(s)	657, 692, 1092, 1250, 1641, 1757, 1824, 2037
SatI	GCNGC	3 cut(s)	437, 1290, 2021
Sau96I	GGNCC	8 cut(s)	414, 896, 910, 986, 1304, 1434, 1807, 2304
ScaI	AGTACT	2 cut(s)	1060, 1997
SchI	GAGTC	3 cut(s)	1847, 2095, 2627
ScrFI	CCNGG	9 cut(s)	188, 301, 441, 1226, 1716, 1836, 2285, 2703, 2724
SduI	GDGCHC	2 cut(s)	522, 1657
SfaNI	GCATC	9 cut(s)	736, 796, 829, 1020, 1059, 1398, 1552, 1939, 2234
SfcI	CTRYAG	2 cut(s)	1018, 2601
SfuI	TTCGAA	1 cut(s)	649
SgrAI	CRCCGGYG	1 cut(s)	2477
SinI	GGWCC	5 cut(s)	896, 910, 986, 1304, 1807
SmiMI	CAYNNNNRTG	4 cut(s)	458, 959, 1172, 1745
SmlI	CTYRAG	2 cut(s)	883, 2455
SmoI	CTYRAG	2 cut(s)	883, 2455
SpeI	ACTAGT	1 cut(s)	1169
SspI	AATATT	1 cut(s)	655
SspMI	CTAG	6 cut(s)	122, 1170, 1398, 1848, 2844, 2946
SstI	GAGCTC	1 cut(s)	522
StyD4I	CCNGG	9 cut(s)	186, 299, 439, 1224, 1714, 1834, 2283, 2701, 2722
StyI	CCWWGG	2 cut(s)	252, 1607
TaaI	ACNGT	9 cut(s)	1022, 1079, 1085, 1703, 1820, 2414, 2562, 2669, 2746
TaiI	ACGT	4 cut(s)	26, 150, 363, 2092
TaqII	GACCGA	1 cut(s)	2506
TatI	WGTACW	8 cut(s)	165, 874, 1058, 1142, 1995, 2266, 2317, 2368
TauI	GCSGC	1 cut(s)	439
TfiI	GAWTC	7 cut(s)	156, 1349, 1564, 1726, 2179, 2348, 2405
Tru1I	TTAA	8 cut(s)	657, 692, 1092, 1250, 1641, 1757, 1824, 2037
Tru9I	TTAA	8 cut(s)	657, 692, 1092, 1250, 1641, 1757, 1824, 2037
TscAI	CASTG	4 cut(s)	1027, 2116, 2787, 2855
TseFI	GTSAC	4 cut(s)	2107, 2335, 2630, 2846
TseI	GCWGC	2 cut(s)	1289, 2020
Tsp45I	GTSAC	4 cut(s)	2107, 2335, 2630, 2846
TspDTI	ATGAA	5 cut(s)	34, 537, 1280, 1371, 1569
TspGWI	ACGGA	4 cut(s)	281, 495, 2142, 2249
TspRI	CASTG	4 cut(s)	1027, 2116, 2787, 2855
Van91I	CCANNNNNTGG	1 cut(s)	2111
VneI	GTGCAC	1 cut(s)	1653
VpaK11BI	GGWCC	5 cut(s)	896, 910, 986, 1304, 1807
XapI	RAATTY	3 cut(s)	2069, 2581, 2737
XceI	RCATGY	1 cut(s)	1636
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2779
XmiI	GTMKAC	2 cut(s)	584, 1698
XmnI	GAANNNNTTC	1 cut(s)	449
XspI	CTAG	6 cut(s)	122, 1170, 1398, 1848, 2844, 2946
ZraI	GACGTC	1 cut(s)	361
ZrmI	AGTACT	2 cut(s)	1060, 1997
Zsp2I	ATGCAT	2 cut(s)	844, 2227

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.