Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0002222

Physical Location & Seq

Genomic Coordinates Forward (+)

3610320 .. 3615664

Gene Structure 5345 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG050700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2478 bp

ATGGCCATATTTGATACTGGTATTCGAGCTAATCACCCGCACTTTCGAAATATTAAGGAGCGGACAAACTGGACAAATGAGGACACATTAAACGACAATCTTGGACATGGGACATTTGTTGCTGGTGTTGTTGCTGGTGTAGATGCAGAGTGTCTTGGATTTGCTCCTGATACAGAGATATATGCGTTTCGTGTGTTTACAGATGCACAGGTATCATACACATCATGGTTCCTTGATGCATTCAACTATGCCATTGCGACCAATATGGATGTACTGAACTTGAGCATAGGTGGACCTGATTACTTGGACCTCCCATTTGTGGAGAAGGTCTGGGAAATAACAGCAAACAACATAATTATGGTTTCAGCAATTGGAAACGATGGACCGCTTTATGGGACTCTAAATAATCCAGCAGACCAAAGTGATGTCATTGGTGTTGGTGGCATCGACTACAGTGATCACATTGCTTCTTTTTCCTCACGAGGCATGAGTACTTGGGAGATGCCGCACGGTTACGGTCGTGTTAAGCCAGATGTCGTTGCATATGGACGGGAAATTATGGGATCCAAGATCAGTACAGGCTGTAAAAGCTTATCTGGCACTAGTGTGGCAAGTCCTGTGGTTGCTGGTATGGTTTGTCTGCTTGTGAGTGTTATCCCTGAAAGCAGTCGGAAGGATACTTTAAATCCAGCAAGCATGAAACAAGCATTGGTTGAGGGTGCTGCAAAACTCGGTGGTCCAAATATGTATGAGCAGGGTGCAGGGAGGGTTGATCTGTTAGAATCATATGAAATCCTGAAGAATTACCAACCTCGTGCCAGCATCTTCCCTAGTATTCTTGATTATACAGACTGCCCATACTCTTGGCCCTTCTGTCGTCAGCCACTTTATGCTGGTGCCATGCCTGTTATCTTCAATGCTACCATTCTAAATGGAATGGGTGTAATTGGCTATGTTGAAAGTCCACCAACTTGGCATCCTTCAGATGAAGTAGGGAATCTTCTAAGTATTCACTTTACATATTCTAATGTTATCTGGCCTTGGACAGGTTATTTAGCGCTTCACATGCAAATTAAGGACGAAGGTGCACAATTTTCAGGAGAGATTAATGGCAATGTTACTCTTAGGGTATACAGTCCTCCATCTCCAGGAGAAAAGAATCTTCGGATTAGCACTTGTGTGCTTCAGTTAAGATTGAAGATTGTTCCAACTCCACCAAGATCAAAACGAGTTTTGTGGGACCAGTTTCACAGTATTAAATACCCTCCAGGTTACATCCCTAGAGACTCTTTGGATGTTCGCAATGACATTCTTGACTGGCATGGAGATCACCTGCATACAAATTTTCACATTATGTTCAACATGTTACGAGATGCTGGGTATTATGTAGAAACACTTGGTTCGCCTCTTACTTGCTTTGATGCTCTTCGATATGGGACACTTCTTCTGGTAGATCTTGAAGAAGAGTACTTTCAGGAGGAGATTGAGAAGCTGCAAGATGATGTTCTTAATTCTGGACTGGGATTGGTTGTGTTTTCTGAGTGGTATAATGTAGACACGATGGTGAAGATGAGATTCTTTGATGATAACACACGTAGCTGGTGGACTCCAGTCACTGGAGGTGCAAATGTTCCAGCTTTGAACGACCTGCTGGCTCCGTTTGGGATTGCATTTGGAGATAAGATTCTGAATGGTGATTTTTCTATCAATGGTGAGCAAAGTCGGTATGCATCTGGAACGGATATTGTGAGGTTTCCGAAAGGGGGTTATGTACACAAGTTCCCTTTCCTGGATAGCTCTGAAAGTGGGGCCACTCAGAATGTACTTCGGGATCCTGAGGTGACAATGGCAGATTCTCCCATTCTTGGTCTTGTACAGGTGGGTGAAGGTCGTGTTGTAGTGTATGGAGATTCTAACTGTTTGGACAGCAGTCATATGGTAACTAATTGTTATTGGCTCTTGAGAAAAATGTTAGATTTCACCGGTGGAAACATCAGAGATCCTGTGCTTTTTTCAAGCTCGGTCAAACAAAGTTCACCTTTATATATGGAAGATAACCAATTACCGTCTCGTAGAACAGATGTGAATTTTTCTACATATTCCGCTGTAGTGGGGAAGGAATTGATCTGTGGGAGTGACTCTGTATTTGAAATATGGGGGACTAAAGGTTACAGTTTACAGGTCAGGGGAACAAACAGAAGATTACCAGGCTCTTCTGTGTCTAATCCAGGTAGAGGTTTGAATTCTACTGTCAAGGCTTCCCATTTGGAGCATTCCAAGTTGACACTGGGAAATAGAAGTGATCCTTCGGAATATAGGGATGAGCTTGATATGCCTGTGTTAGTTGCTAGTCACTGGCTTGTTCCTGCAATAATTGCCGTAACTGGTTTGGTGGTATTTTTGAGCTTCTGGAGAATTAGACAGAAGCGACGACGACGAAGAAAAGGATCTAGCTCTGGTCGATATTCTAATTTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0004252 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005788 GO:0005789 GO:0005794 GO:0006464 GO:0006508 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0006984 GO:0006986 GO:0006996 GO:0007033 GO:0007040 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008237 GO:0009628 GO:0009651 GO:0009889 GO:0009987 GO:0010033 GO:0012505 GO:0016020 GO:0016043 GO:0016787 GO:0017171 GO:0019216 GO:0019218 GO:0019222 GO:0019538 GO:0023052 GO:0030968 GO:0031090 GO:0031293 GO:0031974 GO:0031984 GO:0033554 GO:0033619 GO:0034620 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036500 GO:0042175 GO:0042221 GO:0042538 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043687 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045540 GO:0046890 GO:0050789 GO:0050794 GO:0050810 GO:0050896 GO:0051716 GO:0062012 GO:0065007 GO:0070011 GO:0070013 GO:0070887 GO:0071310 GO:0071704 GO:0071840 GO:0080090 GO:0080171 GO:0090181 GO:0098588 GO:0098791 GO:0098827 GO:0106118 GO:0140096 GO:1901564 GO:1902930

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Peptidase_S8

Protein Analysis

825

Amino Acids

91.51

Weight (kDa)

5.51

Isoelectric Point (pI)

42.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S8	PF00082	2 - 248	1.5e-40	Subtilase family
MBTPS1_3rd	PF23094	276 - 404	2e-54	MBTPS1, third Ig-like domain
MBTPS1_4th	PF23090	405 - 676	3.6e-146	MBTPS1, fourth GATase-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002222)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19660 AT5G19660 AT5G19660
fragaria_vesca	FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670
malus_domestica	MD02G1045500.v1.1 MD15G1183900.v1.1
prunus_persica	Prupe.7G232300_v2.0.a1 Prupe.7G232300_v2.0.a1
pyrus_communis	pycom02g03790 pycom02g03800 pycom02g03810 pycom15g16550
rosa_chinensis	RchiOBHm_Chr2g0090281
rosa_laevigata	RLG00000008526 RLG00000016094 RLG00000021848 RLG00000032088
rosa_multiflora	Rmu_sc0000714.1_g000011 Rmu_sc0004834.1_g000013 Rmu_sc0005276.1_g000005
rosa_roxburghii	Rroxscaffold_2G00085240 Rroxscaffold_2G00151320
rosa_rugosa	Rorug02G0006200
rosa_samantha	Rh1DG037900 Rh2AG052100 Rh2BG050700 Rh2CG052900 Rh2DG052000 Rh5AG367600 Rh5AG473400 Rh5BG377700 Rh5BG493700 Rh5CG401100 Rh5CG517700 Rh5DG391000 Rh5DG505700
rosa_wichuraiana	Rw2G004480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1341
Acc36I	ACCTGC	2 cut(s)	1341, 1656
AccB1I	GGYRCC	1 cut(s)	896
AccB7I	CCANNNNNTGG	1 cut(s)	1616
AccBSI	CCGCTC	1 cut(s)	61
AccI	GTMKAC	2 cut(s)	1131, 1554
AciI	CCGC	5 cut(s)	38, 61, 386, 506, 2103
AclWI	GGATC	7 cut(s)	558, 571, 1826, 1839, 1994, 2297, 2455
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	3 cut(s)	1342, 2086, 2242
AcuI	CTGAAG	3 cut(s)	818, 966, 1169
AfaI	GTAC	7 cut(s)	273, 493, 577, 1469, 1773, 1824, 1875
AfeI	AGCGCT	1 cut(s)	1059
AfiI	CCNNNNNNNGG	9 cut(s)	319, 392, 1046, 1148, 1616, 1763, 1789, 1865, 2234
AflIII	ACRYGT	2 cut(s)	1362, 1592
AgeI	ACCGGT	1 cut(s)	1982
AhlI	ACTAGT	1 cut(s)	602
AjnI	CCWGG	5 cut(s)	1147, 1267, 1788, 2206, 2227
AjuI	GAANNNNNNNTTGG	2 cut(s)	692, 724
AleI	CACNNNNGTG	3 cut(s)	605, 1178, 1562
Alw21I	GWGCWC	1 cut(s)	1090
Alw26I	GTCTC	2 cut(s)	1278, 2073
Alw44I	GTGCAC	1 cut(s)	1086
AlwI	GGATC	7 cut(s)	558, 571, 1826, 1839, 1994, 2297, 2455
AlwNI	CAGNNNCTG	1 cut(s)	1616
Aor51HI	AGCGCT	1 cut(s)	1059
AoxI	GGCC	4 cut(s)	3, 866, 1037, 1809
ApaLI	GTGCAC	1 cut(s)	1086
ApeKI	GCWGC	2 cut(s)	722, 1492
ApoI	RAATTY	3 cut(s)	1342, 2086, 2242
ArsI	GACNNNNNNTTYG	4 cut(s)	299, 331, 2129, 2161
AseI	ATTAAT	1 cut(s)	1107
AsiGI	ACCGGT	1 cut(s)	1982
AspLEI	GCGC	1 cut(s)	1060
AspS9I	GGNCC	7 cut(s)	293, 307, 383, 737, 867, 1240, 1809
AsuHPI	GGTGA	9 cut(s)	26, 1322, 1576, 1706, 1724, 1852, 1895, 1972, 2028
AsuII	TTCGAA	1 cut(s)	46
AvaII	GGWCC	5 cut(s)	293, 307, 383, 737, 1240
AxyI	CCTNAGG	1 cut(s)	1836
BaeGI	GKGCMC	1 cut(s)	1090
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	2 cut(s)	563, 1831
BanI	GGYRCC	1 cut(s)	896
BarI	GAAGNNNNNNTAC	2 cut(s)	984, 1016
BauI	CACGAG	2 cut(s)	480, 813
Bbv12I	GWGCWC	1 cut(s)	1090
BbvI	GCAGC	2 cut(s)	709, 1479
BccI	CCATC	3 cut(s)	374, 1150, 1555
BceAI	ACGGC	1 cut(s)	2363
BciT130I	CCWGG	5 cut(s)	1149, 1269, 1790, 2208, 2229
BciVI	GTATCC	1 cut(s)	670
BclI	TGATCA	1 cut(s)	457
BcoDI	GTCTC	2 cut(s)	1278, 2073
BcuI	ACTAGT	1 cut(s)	602
BfaI	CTAG	5 cut(s)	603, 831, 1281, 2349, 2451
BfmI	CTRYAG	2 cut(s)	451, 2106
BfoI	RGCGCY	1 cut(s)	1061
BfuAI	ACCTGC	2 cut(s)	1341, 1656
BfuI	GTATCC	1 cut(s)	670
BglII	AGATCT	1 cut(s)	1453
BisI	GCNGC	3 cut(s)	506, 723, 1493
BlsI	GCNGC	3 cut(s)	507, 724, 1494
BmcAI	AGTACT	2 cut(s)	493, 1469
Bme1390I	CCNGG	5 cut(s)	1149, 1269, 1790, 2208, 2229
Bme18I	GGWCC	5 cut(s)	293, 307, 383, 737, 1240
BmgT120I	GGNCC	7 cut(s)	293, 307, 383, 737, 867, 1240, 1809
BmiI	GGNNCC	7 cut(s)	230, 565, 898, 1241, 1656, 1810, 1833
BmrFI	CCNGG	5 cut(s)	1149, 1269, 1790, 2208, 2229
BmrI	ACTGGG	2 cut(s)	1529, 2297
BmuI	ACTGGG	2 cut(s)	1529, 2297
BoxI	GACNNNNGTC	2 cut(s)	1610, 1929
BpmI	CTGGAG	5 cut(s)	1131, 1251, 1593, 1638, 2431
Bpu14I	TTCGAA	1 cut(s)	46
BpuEI	CTTGAG	2 cut(s)	301, 1981
BsaAI	YACGTR	1 cut(s)	1595
BsaJI	CCNNGG	1 cut(s)	1040
BsaWI	WCCGGW	1 cut(s)	1982
Bsc4I	CCNNNNNNNGG	9 cut(s)	319, 392, 1046, 1148, 1616, 1763, 1789, 1865, 2234
Bse118I	RCCGGY	1 cut(s)	1982
Bse21I	CCTNAGG	1 cut(s)	1836
Bse3DI	GCAATG	4 cut(s)	252, 462, 1120, 1309
BseBI	CCWGG	5 cut(s)	1149, 1269, 1790, 2208, 2229
BseDI	CCNNGG	1 cut(s)	1040
BseGI	GGATG	5 cut(s)	274, 976, 1275, 1300, 2326
BseLI	CCNNNNNNNGG	9 cut(s)	319, 392, 1046, 1148, 1616, 1763, 1789, 1865, 2234
BseMI	GCAATG	4 cut(s)	252, 462, 1120, 1309
BseMII	CTCAG	3 cut(s)	1530, 1827, 1829
BseRI	GAGGAG	1 cut(s)	1493
BseSI	GKGCMC	1 cut(s)	1090
BseXI	GCAGC	2 cut(s)	709, 1479
BseYI	CCCAGC	1 cut(s)	1376
BsgI	GTGCAG	1 cut(s)	780
Bsh1285I	CGRYCG	1 cut(s)	520
BshFI	GGCC	4 cut(s)	5, 868, 1039, 1811
BshNI	GGYRCC	1 cut(s)	896
BshTI	ACCGGT	1 cut(s)	1982
BsiEI	CGRYCG	1 cut(s)	520
BsiHKAI	GWGCWC	1 cut(s)	1090
BsiSI	CCGG	1 cut(s)	1983
BslFI	GGGAC	5 cut(s)	124, 409, 1253, 1450, 2173
BslI	CCNNNNNNNGG	9 cut(s)	319, 392, 1046, 1148, 1616, 1763, 1789, 1865, 2234
BsmAI	GTCTC	2 cut(s)	1278, 2073
BsmBI	CGTCTC	1 cut(s)	2073
BsmFI	GGGAC	5 cut(s)	124, 409, 1253, 1450, 2173
BsmI	GAATGC	2 cut(s)	239, 2272
BsnI	GGCC	4 cut(s)	5, 868, 1039, 1811
Bsp119I	TTCGAA	1 cut(s)	46
Bsp1286I	GDGCHC	1 cut(s)	1090
Bsp1407I	TGTACA	2 cut(s)	1771, 1873
BspACI	CCGC	5 cut(s)	38, 61, 386, 506, 2103
BspANI	GGCC	4 cut(s)	5, 868, 1039, 1811
BspCNI	CTCAG	3 cut(s)	1531, 1828, 1828
BspLI	GGNNCC	7 cut(s)	230, 565, 898, 1241, 1656, 1810, 1833
BspMI	ACCTGC	2 cut(s)	1341, 1656
BspPI	GGATC	7 cut(s)	558, 571, 1826, 1839, 1994, 2297, 2455
BspQI	GCTCTTC	2 cut(s)	1431, 2218
BspT104I	TTCGAA	1 cut(s)	46
BspT107I	GGYRCC	1 cut(s)	896
BsrBI	CCGCTC	1 cut(s)	61
BsrDI	GCAATG	4 cut(s)	252, 462, 1120, 1309
BsrFI	RCCGGY	1 cut(s)	1982
BsrGI	TGTACA	2 cut(s)	1771, 1873
BssAI	RCCGGY	1 cut(s)	1982
BssECI	CCNNGG	1 cut(s)	1040
BssNAI	GTATAC	1 cut(s)	1132
BssSI	CACGAG	2 cut(s)	480, 813
BssT1I	CCWWGG	1 cut(s)	1040
Bst1107I	GTATAC	1 cut(s)	1132
Bst2BI	CACGAG	2 cut(s)	480, 813
Bst2UI	CCWGG	5 cut(s)	1149, 1269, 1790, 2208, 2229
Bst4CI	ACNGT	9 cut(s)	455, 512, 518, 1136, 1253, 1919, 2067, 2174, 2251
Bst6I	CTCTTC	3 cut(s)	1431, 1458, 2218
BstAPI	GCANNNNNTGC	1 cut(s)	2375
BstAUI	TGTACA	2 cut(s)	1771, 1873
BstBAI	YACGTR	1 cut(s)	1595
BstBI	TTCGAA	1 cut(s)	46
BstC8I	GCNNGC	3 cut(s)	694, 820, 1653
BstDEI	CTNAG	5 cut(s)	1004, 1124, 1539, 1815, 1836
BstENI	CCTNNNNNAGG	1 cut(s)	1044
BstF5I	GGATG	5 cut(s)	274, 976, 1275, 1300, 2326
BstH2I	RGCGCY	1 cut(s)	1061
BstHHI	GCGC	1 cut(s)	1060
BstMAI	GTCTC	2 cut(s)	1278, 2073
BstMCI	CGRYCG	1 cut(s)	520
BstMWI	GCNNNNNNNGC	5 cut(s)	588, 597, 1066, 2332, 2375
BstNI	CCWGG	5 cut(s)	1149, 1269, 1790, 2208, 2229
BstNSI	RCATGY	2 cut(s)	1069, 1366
BstPAI	GACNNNNGTC	2 cut(s)	1610, 1929
BstSCI	CCNGG	5 cut(s)	1147, 1267, 1788, 2206, 2227
BstSFI	CTRYAG	2 cut(s)	451, 2106
BstSLI	GKGCMC	1 cut(s)	1090
BstV1I	GCAGC	2 cut(s)	709, 1479
BstX2I	RGATCY	5 cut(s)	563, 1453, 1831, 1999, 2447
BstXI	CCANNNNNNTGG	2 cut(s)	864, 972
BstYI	RGATCY	5 cut(s)	563, 1453, 1831, 1999, 2447
BstZ17I	GTATAC	1 cut(s)	1132
Bsu36I	CCTNAGG	1 cut(s)	1836
BsuI	GTATCC	1 cut(s)	670
BsuRI	GGCC	4 cut(s)	5, 868, 1039, 1811
BtsCI	GGATG	5 cut(s)	274, 976, 1275, 1300, 2326
BtsIMutI	CAGTG	4 cut(s)	460, 1614, 2285, 2353
BveI	ACCTGC	2 cut(s)	1341, 1656
Cac8I	GCNNGC	3 cut(s)	694, 820, 1653
CaiI	CAGNNNCTG	1 cut(s)	1616
CfoI	GCGC	1 cut(s)	1060
Cfr10I	RCCGGY	1 cut(s)	1982
Cfr13I	GGNCC	7 cut(s)	293, 307, 383, 737, 867, 1240, 1809
Csp6I	GTAC	7 cut(s)	272, 492, 576, 1468, 1772, 1823, 1874
CspAI	ACCGGT	1 cut(s)	1982
CspCI	CAANNNNNGTGG	2 cut(s)	600, 635
CviAII	CATG	8 cut(s)	107, 225, 487, 697, 901, 1066, 1322, 1363
CviQI	GTAC	7 cut(s)	272, 492, 576, 1468, 1772, 1823, 1874
DdeI	CTNAG	5 cut(s)	1004, 1124, 1539, 1815, 1836
DraI	TTTAAA	1 cut(s)	684
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	3 cut(s)	1431, 1458, 2218
EarI	CTCTTC	3 cut(s)	1431, 1458, 2218
Eco130I	CCWWGG	1 cut(s)	1040
Eco47I	GGWCC	5 cut(s)	293, 307, 383, 737, 1240
Eco47III	AGCGCT	1 cut(s)	1059
Eco57I	CTGAAG	3 cut(s)	818, 966, 1169
Eco81I	CCTNAGG	1 cut(s)	1836
EcoNI	CCTNNNNNAGG	1 cut(s)	1044
EcoRI	GAATTC	1 cut(s)	2242
EcoRII	CCWGG	5 cut(s)	1147, 1267, 1788, 2206, 2227
EcoT14I	CCWWGG	1 cut(s)	1040
EcoT22I	ATGCAT	2 cut(s)	241, 1732
ErhI	CCWWGG	1 cut(s)	1040
Esp3I	CGTCTC	1 cut(s)	2073
FaeI	CATG	8 cut(s)	110, 228, 490, 700, 904, 1069, 1325, 1366
FalI	AAGNNNNNCTT	4 cut(s)	2023, 2055, 2290, 2322
FaqI	GGGAC	5 cut(s)	124, 409, 1253, 1450, 2173
FatI	CATG	8 cut(s)	106, 224, 486, 696, 900, 1065, 1321, 1362
FauI	CCCGC	1 cut(s)	45
FauNDI	CATATG	3 cut(s)	544, 787, 1935
FbaI	TGATCA	1 cut(s)	457
FblI	GTMKAC	2 cut(s)	1131, 1554
Fnu4HI	GCNGC	3 cut(s)	506, 723, 1493
FokI	GGATG	5 cut(s)	281, 963, 1262, 1307, 2333
Fsp4HI	GCNGC	3 cut(s)	506, 723, 1493
FspBI	CTAG	5 cut(s)	603, 831, 1281, 2349, 2451
GlaI	GCGC	1 cut(s)	1059
GluI	GCNGC	3 cut(s)	506, 723, 1493
GsaI	CCCAGC	1 cut(s)	1380
GsuI	CTGGAG	5 cut(s)	1131, 1251, 1593, 1638, 2431
HaeII	RGCGCY	1 cut(s)	1061
HaeIII	GGCC	4 cut(s)	5, 868, 1039, 1811
HapII	CCGG	1 cut(s)	1983
HhaI	GCGC	1 cut(s)	1060
Hin1II	CATG	8 cut(s)	110, 228, 490, 700, 904, 1069, 1325, 1366
Hin6I	GCGC	1 cut(s)	1058
HinP1I	GCGC	1 cut(s)	1058
HincII	GTYRAC	1 cut(s)	2283
HindII	GTYRAC	1 cut(s)	2283
HindIII	AAGCTT	1 cut(s)	589
HpaII	CCGG	1 cut(s)	1983
HphI	GGTGA	9 cut(s)	26, 1322, 1576, 1706, 1724, 1852, 1895, 1972, 2028
Hpy99I	CGWCG	3 cut(s)	2433, 2436, 2439
HpyAV	CCTTC	8 cut(s)	319, 667, 880, 990, 1076, 1880, 2110, 2316
HpyCH4III	ACNGT	9 cut(s)	455, 512, 518, 1136, 1253, 1919, 2067, 2174, 2251
HpyCH4IV	ACGT	1 cut(s)	1594
HpyF10VI	GCNNNNNNNGC	5 cut(s)	588, 597, 1066, 2332, 2375
HpyF3I	CTNAG	5 cut(s)	1004, 1124, 1539, 1815, 1836
HpySE526I	ACGT	1 cut(s)	1594
Hsp92II	CATG	8 cut(s)	110, 228, 490, 700, 904, 1069, 1325, 1366
HspAI	GCGC	1 cut(s)	1058
Ksp22I	TGATCA	1 cut(s)	457
LguI	GCTCTTC	2 cut(s)	1431, 2218
LmnI	GCTCC	4 cut(s)	58, 169, 1660, 2269
Lsp1109I	GCAGC	2 cut(s)	709, 1479
MaeI	CTAG	5 cut(s)	603, 831, 1281, 2349, 2451
MaeII	ACGT	1 cut(s)	1594
MbiI	CCGCTC	1 cut(s)	61
MfeI	CAATTG	1 cut(s)	369
MflI	RGATCY	5 cut(s)	563, 1453, 1831, 1999, 2447
MhlI	GDGCHC	1 cut(s)	1090
MlsI	TGGCCA	1 cut(s)	5
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	4 cut(s)	391, 1280, 1600, 2132
MmeI	TCCRAC	2 cut(s)	650, 1232
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	2 cut(s)	241, 1732
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	9 cut(s)	54, 89, 525, 683, 1074, 1107, 1190, 1257, 1509
MslI	CAYNNNNRTG	4 cut(s)	356, 605, 1178, 1562
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	1 cut(s)	2105
MspI	CCGG	1 cut(s)	1983
MspR9I	CCNGG	5 cut(s)	1149, 1269, 1790, 2208, 2229
MunI	CAATTG	1 cut(s)	369
Mva1269I	GAATGC	2 cut(s)	239, 2272
MvaI	CCWGG	5 cut(s)	1149, 1269, 1790, 2208, 2229
MwoI	GCNNNNNNNGC	5 cut(s)	588, 597, 1066, 2332, 2375
NdeI	CATATG	3 cut(s)	544, 787, 1935
NlaIII	CATG	8 cut(s)	110, 228, 490, 700, 904, 1069, 1325, 1366
NlaIV	GGNNCC	7 cut(s)	230, 565, 898, 1241, 1656, 1810, 1833
NmuCI	GTSAC	4 cut(s)	1612, 1840, 2135, 2351
NsiI	ATGCAT	2 cut(s)	241, 1732
NspI	RCATGY	2 cut(s)	1069, 1366
NspV	TTCGAA	1 cut(s)	46
OliI	CACNNNNGTG	3 cut(s)	605, 1178, 1562
PaqCI	CACCTGC	1 cut(s)	1341
PciI	ACATGT	1 cut(s)	1362
PciSI	GCTCTTC	2 cut(s)	1431, 2218
PctI	GAATGC	2 cut(s)	239, 2272
PfeI	GAWTC	7 cut(s)	782, 997, 1159, 1575, 1684, 1853, 1910
PflMI	CCANNNNNTGG	1 cut(s)	1616
PfoI	TCCNGGA	2 cut(s)	1147, 1788
PinAI	ACCGGT	1 cut(s)	1982
PkrI	GCNGC	3 cut(s)	507, 724, 1494
PleI	GAGTC	4 cut(s)	391, 1280, 1600, 2132
PpsI	GAGTC	4 cut(s)	391, 1280, 1600, 2132
Ppu21I	YACGTR	1 cut(s)	1595
PscI	ACATGT	1 cut(s)	1362
PshAI	GACNNNNGTC	2 cut(s)	1610, 1929
PshBI	ATTAAT	1 cut(s)	1107
Psp6I	CCWGG	5 cut(s)	1147, 1267, 1788, 2206, 2227
PspFI	CCCAGC	1 cut(s)	1376
PspGI	CCWGG	5 cut(s)	1147, 1267, 1788, 2206, 2227
PspN4I	GGNNCC	7 cut(s)	230, 565, 898, 1241, 1656, 1810, 1833
PspPI	GGNCC	7 cut(s)	293, 307, 383, 737, 867, 1240, 1809
PstNI	CAGNNNCTG	1 cut(s)	1616
PsuI	RGATCY	5 cut(s)	563, 1453, 1831, 1999, 2447
RsaI	GTAC	7 cut(s)	273, 493, 577, 1469, 1773, 1824, 1875
RsaNI	GTAC	7 cut(s)	272, 492, 576, 1468, 1772, 1823, 1874
RseI	CAYNNNNRTG	4 cut(s)	356, 605, 1178, 1562
SapI	GCTCTTC	2 cut(s)	1431, 2218
SaqAI	TTAA	9 cut(s)	54, 89, 525, 683, 1074, 1107, 1190, 1257, 1509
SatI	GCNGC	3 cut(s)	506, 723, 1493
Sau96I	GGNCC	7 cut(s)	293, 307, 383, 737, 867, 1240, 1809
ScaI	AGTACT	2 cut(s)	493, 1469
SchI	GAGTC	4 cut(s)	391, 1280, 1600, 2132
ScrFI	CCNGG	5 cut(s)	1149, 1269, 1790, 2208, 2229
SduI	GDGCHC	1 cut(s)	1090
SfcI	CTRYAG	2 cut(s)	451, 2106
SfuI	TTCGAA	1 cut(s)	46
SgrAI	CRCCGGYG	1 cut(s)	1982
SinI	GGWCC	5 cut(s)	293, 307, 383, 737, 1240
SmiMI	CAYNNNNRTG	4 cut(s)	356, 605, 1178, 1562
SmlI	CTYRAG	2 cut(s)	280, 1960
SmoI	CTYRAG	2 cut(s)	280, 1960
SpeI	ACTAGT	1 cut(s)	602
SsiI	CCGC	5 cut(s)	38, 61, 386, 506, 2103
SspI	AATATT	1 cut(s)	52
SspMI	CTAG	5 cut(s)	603, 831, 1281, 2349, 2451
StyD4I	CCNGG	5 cut(s)	1147, 1267, 1788, 2206, 2227
StyI	CCWWGG	1 cut(s)	1040
TaaI	ACNGT	9 cut(s)	455, 512, 518, 1136, 1253, 1919, 2067, 2174, 2251
TaiI	ACGT	1 cut(s)	1597
TaqI	TCGA	5 cut(s)	25, 46, 447, 1429, 2461
TaqII	GACCGA	1 cut(s)	2011
TatI	WGTACW	7 cut(s)	271, 491, 575, 1467, 1771, 1822, 1873
TauI	GCSGC	1 cut(s)	508
TfiI	GAWTC	7 cut(s)	782, 997, 1159, 1575, 1684, 1853, 1910
Tru1I	TTAA	9 cut(s)	54, 89, 525, 683, 1074, 1107, 1190, 1257, 1509
Tru9I	TTAA	9 cut(s)	54, 89, 525, 683, 1074, 1107, 1190, 1257, 1509
TscAI	CASTG	4 cut(s)	460, 1621, 2292, 2360
TseFI	GTSAC	4 cut(s)	1612, 1840, 2135, 2351
TseI	GCWGC	2 cut(s)	722, 1492
Tsp45I	GTSAC	4 cut(s)	1612, 1840, 2135, 2351
TspDTI	ATGAA	3 cut(s)	713, 804, 1002
TspGWI	ACGGA	2 cut(s)	1647, 1754
TspRI	CASTG	4 cut(s)	460, 1621, 2292, 2360
Van91I	CCANNNNNTGG	1 cut(s)	1616
VneI	GTGCAC	1 cut(s)	1086
VpaK11BI	GGWCC	5 cut(s)	293, 307, 383, 737, 1240
VspI	ATTAAT	1 cut(s)	1107
XagI	CCTNNNNNAGG	1 cut(s)	1044
XapI	RAATTY	3 cut(s)	1342, 2086, 2242
XceI	RCATGY	2 cut(s)	1069, 1366
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2284
XmiI	GTMKAC	2 cut(s)	1131, 1554
XspI	CTAG	5 cut(s)	603, 831, 1281, 2349, 2451
ZrmI	AGTACT	2 cut(s)	493, 1469
Zsp2I	ATGCAT	2 cut(s)	241, 1732

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.