Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0002222

Physical Location & Seq

Genomic Coordinates Forward (+)

3827348 .. 3832269

Gene Structure 4922 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016094

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3111 bp

ATGACCACTCCTCTGACCGCCACGTCATCACTATCCTTCTTCTTCATCATCGTCTCCATCGCCGTAGCCGTCTCCATCTCTCTCTTCCATTACAAACCCCACCAGAACCGCCCAACCCTCGAGCCCAACGCAACCCTAGATCCTCCTCCACCGCAAAACTACGTCGTTCGATTCGTCGAGTACAAGAGGTGGGAGGATCACCGGCAGTATCTGAAATCGAGTGTGCCGGCGGAGGGATGGGACTGGATCGAGAGGAACAATCCGGCCATGGATTACCCGACGGATTTCGGGCTGGTCCGGATTGACGATGGAGCCAGGGAATTGGTGATTGGGGAGATTGGGAAATTGGGGATGGTGAAGGATGTGAATGCTGACGTCAGGTATGGGAGGGGTTTGCTTAGTGAGGAGGAGAAGAGGGAGAAAGTTGGGGCCTTTGTGGATGGGGAGAAGCGGCCTGGGAAGATGTTCACGGCGATGTCGTTTAGCGAAGACGACGGAGCAGTGAGTAATGGCTCTGTTAGCTGGAAAAGGGAGCTCTTGGCTGAGAGATCTCAAATAACTTCATTATTTGGAGCGGATTTTCTTTGGAGAAAAGGGTATACTGGTACGAAAGTGAAAATGGCCATATTTGATACTGGTATTCGAGCTAATCACCCGCACTTTCGAAATATTAAGGAGCGGACAAACTGGACAAATGAGGATACATTAAACGACAATCTTGGACATGGGACATTTGTTGCTGGTGTTGTTGCTGGTGTAGATGCAGAGTGTCTTGGATTTGCTCCTGATACAGAGATATATGCGTTTCGTGTGTTTACAGATGCACAGGTATCATACACATCATGGTTCCTTGATGCATTCAACTATGCCATTGCGACCAATATGGATGTACTGAACTTGAGCATAGGTGGACCTGATTACTTGGACCTTCCATTTGTGGAGAAGGTCTGGGAAATAACAGCAAACAACATAATTATGGTTTCAGCAATTGGAAACGATGGACCGCTATATGGGACTCTAAATAATCCAGCAGACCAAAGTGATGTCATTGGTGTTGGTGGCATCGACTACAGTGATCACATTGCTTCTTTTTCCTCACGAGGCATGAGTACTTGGGAGATGCCGCATGGTTACGGTCGTGTTAAGCCAGATGTCGTTGCATATGGACGGGAAATTATGGGATCCAAGATCAGTACAGGCTGTAAAAGCTTATCTGGCACTAGTGTGGCAAGTCCTGTGGTTGCTGGTATGGTTTGTCTGCTTGTGAGTGTTATCCCTGAAAGCAGTCGGAAGGATACTTTAAATCCAGCAAGCATGAAACAAGCATTGGTTGAGGGTGCTGCAAAACTCGGTGGTCCAAATATGTATGAGCAGGGTGCAGGGAGGGTTGATCTGTTAGAATCATATGAAATCCTGAAGAATTACCAACCTCGTGCCAGCATCTTCCCTAGTATTCTTGATTATACAGACTGCCCATACTCTTGGCCCTTCTGTCGTCAGCCACTTTATGCTGGTGCCATGCCTGTTATCTTCAATGCTACCATTCTAAATGGAATGGGTGTAATTGGCTATGTTGAAAGTCCACCAACTTGGCATCCTTCAGATGAAGTAGGGAATCTTCTAAGTATTCACTTTACATATTCTAATGTTATCTGGCCTTGGACTGGTTATTTAGCGCTTCACATGCAAATTAAGGACGAAGGTGCACAATTTTCAGGAGAGATTAGCGGCAATGTTACTCTTAGGGTATACAGTCCTCCATCTCCGGGAGAAAAGAATCTTCGGATTAGTACTTGTGTGCTTCAGTTAAGATTGAAGATTGTTCCAACTCCACCAAGATCAAAACGAGTTTTGTGGGACCAGTTTCACAGTATTAAATACCCTCCAGGTTACATCCCTAGAGACTCTTTGGATGTTCGCAATGACATTCTTGACTGGCATGGAGATCACCTGCATACAAATTTTCACATTATGTTCAACATGTTACGAGATGCTGGGTATTATGTAGAAACACTTGGTTCGCCTCTTACTTGCTTTGATGCTCTTCGATATGGGACACTTCTTCTGGTAGATCTTGAAGAAGAGTACTTTCAGGAGGAGATTGAGAAGCTGCGAGATGATGTTCTTAATTCTGGACTGGGATTGGTTGTGTTTTCTGAGTGGTATAATGTAGACACGATGGTGAAGATGAGATTCTTTGATGATAACACACGTAGCTGGTGGACTCCAGTCACTGGAGGTGCAAATGTTCCAGCGTTGAACGACCTGCTGGCTCCGTTTGGAATTGCATTTGGAGATAAGATTCTGAATGGTGATTTTTCTATCAATGGTGAGCAAAGTCGGTATGCATCTGGAACAGATATTGTGAGGTTTCCAAAAGGGGGTTATGTACACAAGTTCCCTTTCCTGGATAGCTCTGAAAGTGGGGCCACTCAGAATGTACTTCGGGATCCTGAGGTGACAATGGCAGATTCTCCCATTCTTGGTCTTGTACAGGTGGGTGAAGGTCGTGTTGTAGTGTATGGAGATTCTAACTGTTTGGACAGTAGTCATATGGTAACTAATTGTTATTGGCTCTTGAGAAAAATGTTAGATTTCACCGGTGGAAACATCAGAGATCCTGTGCTTTTTTCAAGCTCGGTCAAACAAAGTTCACCTTTATATATGGAAGATAACCAATTACCGTCTCGTAGAACAGATGTGAATTTTTCTACATATTCCGCTGTAGTGGGGAAGGAATTGATCTGTGGGAGTGACTCTGTATTTGAAATATGGGGGACTAAAGGTTACAGTTTACAGGTCAGGGGAACAAACAGAAGATTACCAGGCTCTTCTGTGTCTAATCCAGGTAGAGGTTTGAATTCTACTGTCGAGGCTTCCTATTTGAAGCATTCTGAGTTGACACTGGGAAATAGAAGTGATCCTTCGGGATACTTGGGAATATTCTATAGGGATGAGCTTGATATGCCTGTGTTAGTTGCTAGTCACTGGCTTGTTCCTGCAATAATTGCCGTAACTGGTTTGGTGGTATTTTTGAGCTTCTGGAGAATTAGACAGAAGCGACGACGACGAAGAAAAGGACCTAGCTCTGGTCGATATTCTAATTTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0004252 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005788 GO:0005789 GO:0005794 GO:0006464 GO:0006508 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0006984 GO:0006986 GO:0006996 GO:0007033 GO:0007040 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008237 GO:0009628 GO:0009651 GO:0009889 GO:0009987 GO:0010033 GO:0012505 GO:0016020 GO:0016043 GO:0016787 GO:0017171 GO:0019216 GO:0019218 GO:0019222 GO:0019538 GO:0023052 GO:0030968 GO:0031090 GO:0031293 GO:0031974 GO:0031984 GO:0033554 GO:0033619 GO:0034620 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036500 GO:0042175 GO:0042221 GO:0042538 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043687 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045540 GO:0046890 GO:0050789 GO:0050794 GO:0050810 GO:0050896 GO:0051716 GO:0062012 GO:0065007 GO:0070011 GO:0070013 GO:0070887 GO:0071310 GO:0071704 GO:0071840 GO:0080090 GO:0080171 GO:0090181 GO:0098588 GO:0098791 GO:0098827 GO:0106118 GO:0140096 GO:1901564 GO:1902930

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Peptidase_S8

Protein Analysis

1037

Amino Acids

115.34

Weight (kDa)

5.61

Isoelectric Point (pI)

42.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MBTP1_N	PF23001	52 - 124	4.3e-13	Membrane-bound Site-1 Protease Family N-terminal domain
Peptidase_S8	PF00082	202 - 454	8.6e-42	Subtilase family
MBTPS1_3rd	PF23094	482 - 610	2.7e-54	MBTPS1, third Ig-like domain
MBTPS1_4th	PF23090	611 - 882	2.3e-146	MBTPS1, fourth GATase-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002222)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19660 AT5G19660 AT5G19660
fragaria_vesca	FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670 FvH4_1g04670
malus_domestica	MD02G1045500.v1.1 MD15G1183900.v1.1
prunus_persica	Prupe.7G232300_v2.0.a1 Prupe.7G232300_v2.0.a1
pyrus_communis	pycom02g03790 pycom02g03800 pycom02g03810 pycom15g16550
rosa_chinensis	RchiOBHm_Chr2g0090281
rosa_laevigata	RLG00000008526 RLG00000016094 RLG00000021848 RLG00000032088
rosa_multiflora	Rmu_sc0000714.1_g000011 Rmu_sc0004834.1_g000013 Rmu_sc0005276.1_g000005
rosa_roxburghii	Rroxscaffold_2G00085240 Rroxscaffold_2G00151320
rosa_rugosa	Rorug02G0006200
rosa_samantha	Rh1DG037900 Rh2AG052100 Rh2BG050700 Rh2CG052900 Rh2DG052000 Rh5AG367600 Rh5AG473400 Rh5BG377700 Rh5BG493700 Rh5CG401100 Rh5CG517700 Rh5DG391000 Rh5DG505700
rosa_wichuraiana	Rw2G004480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1959
AasI	GACNNNNNNGTC	1 cut(s)	22
AatII	GACGTC	1 cut(s)	378
Acc36I	ACCTGC	2 cut(s)	1959, 2274
AccB1I	GGYRCC	1 cut(s)	1514
AccB7I	CCANNNNNTGG	1 cut(s)	2234
AccBSI	CCGCTC	2 cut(s)	575, 679
AccI	GTMKAC	3 cut(s)	599, 1749, 2172
AccIII	TCCGGA	1 cut(s)	297
AclWI	GGATC	9 cut(s)	134, 204, 254, 1176, 1189, 2444, 2457, 2612, 2915
AcoI	YGGCCR	2 cut(s)	264, 621
AcsI	RAATTY	3 cut(s)	1960, 2704, 2860
AcuI	CTGAAG	3 cut(s)	1436, 1584, 1787
AcyI	GRCGYC	1 cut(s)	375
AfeI	AGCGCT	1 cut(s)	1677
AflIII	ACRYGT	2 cut(s)	1980, 2210
AgeI	ACCGGT	1 cut(s)	2600
AhlI	ACTAGT	1 cut(s)	1220
AjiI	CACGTC	1 cut(s)	24
AjnI	CCWGG	6 cut(s)	314, 454, 1885, 2406, 2824, 2845
AjuI	GAANNNNNNNTTGG	4 cut(s)	312, 344, 1310, 1342
AleI	CACNNNNGTG	2 cut(s)	1223, 2180
Alw21I	GWGCWC	2 cut(s)	537, 1708
Alw26I	GTCTC	4 cut(s)	58, 76, 1896, 2691
Alw44I	GTGCAC	1 cut(s)	1704
AlwI	GGATC	9 cut(s)	134, 204, 254, 1176, 1189, 2444, 2457, 2612, 2915
AlwNI	CAGNNNCTG	2 cut(s)	211, 2234
Ama87I	CYCGRG	1 cut(s)	119
Aor13HI	TCCGGA	1 cut(s)	297
Aor51HI	AGCGCT	1 cut(s)	1677
AoxI	GGCC	7 cut(s)	264, 429, 452, 621, 1484, 1655, 2427
ApaLI	GTGCAC	1 cut(s)	1704
ApeKI	GCWGC	2 cut(s)	1340, 2110
ApoI	RAATTY	3 cut(s)	1960, 2704, 2860
ArsI	GACNNNNNNTTYG	4 cut(s)	917, 949, 2747, 2779
AsiGI	ACCGGT	1 cut(s)	2600
Asp700I	GAANNNNTTC	1 cut(s)	464
AspLEI	GCGC	1 cut(s)	1678
AsuC2I	CCSGG	1 cut(s)	1767
AsuII	TTCGAA	1 cut(s)	664
AvaI	CYCGRG	1 cut(s)	119
AvaII	GGWCC	7 cut(s)	295, 911, 925, 1001, 1355, 1858, 3080
AxyI	CCTNAGG	1 cut(s)	2454
BaeGI	GKGCMC	1 cut(s)	1708
BaeI	ACNNNNGTAYC	2 cut(s)	597, 630
BalI	TGGCCA	1 cut(s)	623
BamHI	GGATCC	2 cut(s)	1181, 2449
BanI	GGYRCC	1 cut(s)	1514
BanII	GRGCYC	2 cut(s)	126, 537
BarI	GAAGNNNNNNTAC	2 cut(s)	1602, 1634
BauI	CACGAG	2 cut(s)	1098, 1431
BbsI	GAAGAC	1 cut(s)	495
Bbv12I	GWGCWC	2 cut(s)	537, 1708
BbvI	GCAGC	2 cut(s)	1327, 2097
BccI	CCATC	9 cut(s)	65, 83, 231, 302, 346, 434, 992, 1768, 2173
BceAI	ACGGC	4 cut(s)	47, 53, 486, 2996
BciT130I	CCWGG	6 cut(s)	316, 456, 1887, 2408, 2826, 2847
BciVI	GTATCC	3 cut(s)	694, 1288, 2924
BclI	TGATCA	1 cut(s)	1075
BcnI	CCSGG	1 cut(s)	1767
BcoDI	GTCTC	4 cut(s)	58, 76, 1896, 2691
BcuI	ACTAGT	1 cut(s)	1220
BfaI	CTAG	6 cut(s)	137, 1221, 1449, 1899, 2982, 3084
BfmI	CTRYAG	3 cut(s)	1069, 2724, 2947
BfoI	RGCGCY	1 cut(s)	1679
BfuAI	ACCTGC	2 cut(s)	1959, 2274
BfuI	GTATCC	3 cut(s)	694, 1288, 2924
BglII	AGATCT	2 cut(s)	548, 2071
BisI	GCNGC	5 cut(s)	452, 1124, 1341, 1729, 2111
BlsI	GCNGC	5 cut(s)	453, 1125, 1342, 1730, 2112
BmcAI	AGTACT	3 cut(s)	1111, 1792, 2087
Bme1390I	CCNGG	7 cut(s)	316, 456, 1767, 1887, 2408, 2826, 2847
Bme18I	GGWCC	7 cut(s)	295, 911, 925, 1001, 1355, 1858, 3080
BmeT110I	CYCGRG	1 cut(s)	119
BmgBI	CACGTC	1 cut(s)	24
BmiI	GGNNCC	9 cut(s)	313, 430, 848, 1183, 1516, 1859, 2274, 2428, 2451
BmrFI	CCNGG	7 cut(s)	316, 456, 1767, 1887, 2408, 2826, 2847
BmrI	ACTGGG	2 cut(s)	2147, 2915
BmuI	ACTGGG	2 cut(s)	2147, 2915
BoxI	GACNNNNGTC	2 cut(s)	2228, 2547
BpiI	GAAGAC	1 cut(s)	495
BpmI	CTGGAG	4 cut(s)	1869, 2211, 2256, 3064
Bpu14I	TTCGAA	1 cut(s)	664
BpuEI	CTTGAG	2 cut(s)	919, 2599
BpuMI	CCSGG	1 cut(s)	1767
BsaAI	YACGTR	1 cut(s)	2213
BsaHI	GRCGYC	1 cut(s)	375
BsaJI	CCNNGG	4 cut(s)	267, 315, 455, 1658
BsaWI	WCCGGW	2 cut(s)	297, 2600
Bse118I	RCCGGY	3 cut(s)	201, 226, 2600
Bse21I	CCTNAGG	1 cut(s)	2454
Bse3DI	GCAATG	4 cut(s)	870, 1080, 1738, 1927
BseAI	TCCGGA	1 cut(s)	297
BseBI	CCWGG	6 cut(s)	316, 456, 1887, 2408, 2826, 2847
BseDI	CCNNGG	4 cut(s)	267, 315, 455, 1658
BseGI	GGATG	9 cut(s)	242, 357, 367, 445, 892, 1594, 1893, 1918, 2959
BseMI	GCAATG	4 cut(s)	870, 1080, 1738, 1927
BseMII	CTCAG	5 cut(s)	534, 2148, 2445, 2447, 2886
BseRI	GAGGAG	4 cut(s)	135, 419, 422, 2111
BseSI	GKGCMC	1 cut(s)	1708
BseXI	GCAGC	2 cut(s)	1327, 2097
BseYI	CCCAGC	1 cut(s)	1994
BsgI	GTGCAG	1 cut(s)	1398
Bsh1285I	CGRYCG	1 cut(s)	1138
BshFI	GGCC	7 cut(s)	266, 431, 454, 623, 1486, 1657, 2429
BshNI	GGYRCC	1 cut(s)	1514
BshTI	ACCGGT	1 cut(s)	2600
BsiEI	CGRYCG	1 cut(s)	1138
BsiHKAI	GWGCWC	2 cut(s)	537, 1708
BsiHKCI	CYCGRG	1 cut(s)	119
BsiSI	CCGG	6 cut(s)	202, 227, 263, 298, 1766, 2601
BslFI	GGGAC	6 cut(s)	254, 742, 1027, 1871, 2068, 2791
BsmAI	GTCTC	4 cut(s)	58, 76, 1896, 2691
BsmBI	CGTCTC	3 cut(s)	58, 76, 2691
BsmFI	GGGAC	6 cut(s)	254, 742, 1027, 1871, 2068, 2791
BsmI	GAATGC	3 cut(s)	373, 857, 2890
BsnI	GGCC	7 cut(s)	266, 431, 454, 623, 1486, 1657, 2429
BsoBI	CYCGRG	1 cut(s)	119
Bsp119I	TTCGAA	1 cut(s)	664
Bsp1286I	GDGCHC	3 cut(s)	126, 537, 1708
Bsp13I	TCCGGA	1 cut(s)	297
Bsp1407I	TGTACA	2 cut(s)	2389, 2491
Bsp19I	CCATGG	1 cut(s)	267
BspANI	GGCC	7 cut(s)	266, 431, 454, 623, 1486, 1657, 2429
BspCNI	CTCAG	5 cut(s)	535, 2149, 2446, 2446, 2887
BspEI	TCCGGA	1 cut(s)	297
BspLI	GGNNCC	9 cut(s)	313, 430, 848, 1183, 1516, 1859, 2274, 2428, 2451
BspMI	ACCTGC	2 cut(s)	1959, 2274
BspPI	GGATC	9 cut(s)	134, 204, 254, 1176, 1189, 2444, 2457, 2612, 2915
BspQI	GCTCTTC	2 cut(s)	2049, 2836
BspT104I	TTCGAA	1 cut(s)	664
BspT107I	GGYRCC	1 cut(s)	1514
BsrBI	CCGCTC	2 cut(s)	575, 679
BsrDI	GCAATG	4 cut(s)	870, 1080, 1738, 1927
BsrFI	RCCGGY	3 cut(s)	201, 226, 2600
BsrGI	TGTACA	2 cut(s)	2389, 2491
BssAI	RCCGGY	3 cut(s)	201, 226, 2600
BssECI	CCNNGG	4 cut(s)	267, 315, 455, 1658
BssNAI	GTATAC	2 cut(s)	600, 1750
BssNI	GRCGYC	1 cut(s)	375
BssSI	CACGAG	2 cut(s)	1098, 1431
BssT1I	CCWWGG	2 cut(s)	267, 1658
Bst1107I	GTATAC	2 cut(s)	600, 1750
Bst2BI	CACGAG	2 cut(s)	1098, 1431
Bst2UI	CCWGG	6 cut(s)	316, 456, 1887, 2408, 2826, 2847
Bst4CI	ACNGT	9 cut(s)	1073, 1136, 1754, 1871, 2537, 2546, 2685, 2792, 2869
Bst6I	CTCTTC	5 cut(s)	89, 407, 2049, 2076, 2836
BstACI	GRCGYC	1 cut(s)	375
BstAPI	GCANNNNNTGC	1 cut(s)	3008
BstAUI	TGTACA	2 cut(s)	2389, 2491
BstBAI	YACGTR	1 cut(s)	2213
BstBI	TTCGAA	1 cut(s)	664
BstC8I	GCNNGC	4 cut(s)	228, 1312, 1438, 2271
BstDEI	CTNAG	8 cut(s)	398, 543, 1622, 1742, 2157, 2433, 2454, 2895
BstDSI	CCRYGG	1 cut(s)	267
BstF5I	GGATG	9 cut(s)	242, 357, 367, 445, 892, 1594, 1893, 1918, 2959
BstH2I	RGCGCY	1 cut(s)	1679
BstHHI	GCGC	1 cut(s)	1678
BstMAI	GTCTC	4 cut(s)	58, 76, 1896, 2691
BstMCI	CGRYCG	1 cut(s)	1138
BstMWI	GCNNNNNNNGC	6 cut(s)	519, 1206, 1215, 1684, 2965, 3008
BstNI	CCWGG	6 cut(s)	316, 456, 1887, 2408, 2826, 2847
BstNSI	RCATGY	2 cut(s)	1687, 1984
BstPAI	GACNNNNGTC	2 cut(s)	2228, 2547
BstSCI	CCNGG	7 cut(s)	314, 454, 1765, 1885, 2406, 2824, 2845
BstSFI	CTRYAG	3 cut(s)	1069, 2724, 2947
BstSLI	GKGCMC	1 cut(s)	1708
BstV1I	GCAGC	2 cut(s)	1327, 2097
BstV2I	GAAGAC	1 cut(s)	495
BstX2I	RGATCY	6 cut(s)	139, 548, 1181, 2071, 2449, 2617
BstXI	CCANNNNNNTGG	3 cut(s)	322, 1482, 1590
BstYI	RGATCY	6 cut(s)	139, 548, 1181, 2071, 2449, 2617
BstZ17I	GTATAC	2 cut(s)	600, 1750
Bsu36I	CCTNAGG	1 cut(s)	2454
BsuI	GTATCC	3 cut(s)	694, 1288, 2924
BsuRI	GGCC	7 cut(s)	266, 431, 454, 623, 1486, 1657, 2429
BtgI	CCRYGG	1 cut(s)	267
BtgZI	GCGATG	2 cut(s)	43, 488
BtrI	CACGTC	1 cut(s)	24
BtsCI	GGATG	9 cut(s)	242, 357, 367, 445, 892, 1594, 1893, 1918, 2959
BtsI	GCAGTG	1 cut(s)	507
BtsIMutI	CAGTG	5 cut(s)	507, 1078, 2232, 2903, 2986
BveI	ACCTGC	2 cut(s)	1959, 2274
Cac8I	GCNNGC	4 cut(s)	228, 1312, 1438, 2271
CaiI	CAGNNNCTG	2 cut(s)	211, 2234
CfoI	GCGC	1 cut(s)	1678
Cfr10I	RCCGGY	3 cut(s)	201, 226, 2600
CspAI	ACCGGT	1 cut(s)	2600
CspCI	CAANNNNNGTGG	2 cut(s)	1218, 1253
DdeI	CTNAG	8 cut(s)	398, 543, 1622, 1742, 2157, 2433, 2454, 2895
DraI	TTTAAA	1 cut(s)	1302
DrdI	GACNNNNNNGTC	1 cut(s)	22
DseDI	GACNNNNNNGTC	1 cut(s)	22
EaeI	YGGCCR	2 cut(s)	264, 621
Eam1104I	CTCTTC	5 cut(s)	89, 407, 2049, 2076, 2836
EarI	CTCTTC	5 cut(s)	89, 407, 2049, 2076, 2836
EciI	GGCGGA	1 cut(s)	245
Ecl136II	GAGCTC	1 cut(s)	535
Eco130I	CCWWGG	2 cut(s)	267, 1658
Eco24I	GRGCYC	2 cut(s)	126, 537
Eco47I	GGWCC	7 cut(s)	295, 911, 925, 1001, 1355, 1858, 3080
Eco47III	AGCGCT	1 cut(s)	1677
Eco53kI	GAGCTC	1 cut(s)	535
Eco57I	CTGAAG	3 cut(s)	1436, 1584, 1787
Eco81I	CCTNAGG	1 cut(s)	2454
Eco88I	CYCGRG	1 cut(s)	119
EcoICRI	GAGCTC	1 cut(s)	535
EcoO109I	RGGNCCY	2 cut(s)	429, 3080
EcoRI	GAATTC	1 cut(s)	2860
EcoRII	CCWGG	6 cut(s)	314, 454, 1885, 2406, 2824, 2845
EcoT14I	CCWWGG	2 cut(s)	267, 1658
EcoT22I	ATGCAT	2 cut(s)	859, 2350
EcoT38I	GRGCYC	2 cut(s)	126, 537
ErhI	CCWWGG	2 cut(s)	267, 1658
Esp3I	CGTCTC	3 cut(s)	58, 76, 2691
FalI	AAGNNNNNCTT	4 cut(s)	2641, 2673, 2908, 2940
FaqI	GGGAC	6 cut(s)	254, 742, 1027, 1871, 2068, 2791
FauI	CCCGC	1 cut(s)	663
FauNDI	CATATG	3 cut(s)	1162, 1405, 2553
FbaI	TGATCA	1 cut(s)	1075
FblI	GTMKAC	3 cut(s)	599, 1749, 2172
Fnu4HI	GCNGC	5 cut(s)	452, 1124, 1341, 1729, 2111
FokI	GGATG	9 cut(s)	249, 364, 374, 452, 899, 1581, 1880, 1925, 2966
FriOI	GRGCYC	2 cut(s)	126, 537
Fsp4HI	GCNGC	5 cut(s)	452, 1124, 1341, 1729, 2111
FspBI	CTAG	6 cut(s)	137, 1221, 1449, 1899, 2982, 3084
GlaI	GCGC	1 cut(s)	1677
GluI	GCNGC	5 cut(s)	452, 1124, 1341, 1729, 2111
GsaI	CCCAGC	1 cut(s)	1998
GsuI	CTGGAG	4 cut(s)	1869, 2211, 2256, 3064
HaeII	RGCGCY	1 cut(s)	1679
HaeIII	GGCC	7 cut(s)	266, 431, 454, 623, 1486, 1657, 2429
HapII	CCGG	6 cut(s)	202, 227, 263, 298, 1766, 2601
HhaI	GCGC	1 cut(s)	1678
Hin1I	GRCGYC	1 cut(s)	375
Hin6I	GCGC	1 cut(s)	1676
HinP1I	GCGC	1 cut(s)	1676
HincII	GTYRAC	1 cut(s)	2901
HindII	GTYRAC	1 cut(s)	2901
HindIII	AAGCTT	1 cut(s)	1207
HpaII	CCGG	6 cut(s)	202, 227, 263, 298, 1766, 2601
Hpy99I	CGWCG	7 cut(s)	167, 179, 283, 497, 3066, 3069, 3072
HpyCH4III	ACNGT	9 cut(s)	1073, 1136, 1754, 1871, 2537, 2546, 2685, 2792, 2869
HpyCH4IV	ACGT	4 cut(s)	23, 162, 375, 2212
HpyF10VI	GCNNNNNNNGC	6 cut(s)	519, 1206, 1215, 1684, 2965, 3008
HpyF3I	CTNAG	8 cut(s)	398, 543, 1622, 1742, 2157, 2433, 2454, 2895
HpySE526I	ACGT	4 cut(s)	23, 162, 375, 2212
Hsp92I	GRCGYC	1 cut(s)	375
HspAI	GCGC	1 cut(s)	1676
Kpn2I	TCCGGA	1 cut(s)	297
KroI	GCCGGC	1 cut(s)	226
KroNI	GCCGGC	1 cut(s)	228
Ksp22I	TGATCA	1 cut(s)	1075
LguI	GCTCTTC	2 cut(s)	2049, 2836
LmnI	GCTCC	7 cut(s)	311, 497, 532, 572, 676, 787, 2278
Lsp1109I	GCAGC	2 cut(s)	1327, 2097
MaeI	CTAG	6 cut(s)	137, 1221, 1449, 1899, 2982, 3084
MaeII	ACGT	4 cut(s)	23, 162, 375, 2212
MbiI	CCGCTC	2 cut(s)	575, 679
MfeI	CAATTG	1 cut(s)	987
MflI	RGATCY	6 cut(s)	139, 548, 1181, 2071, 2449, 2617
MhlI	GDGCHC	3 cut(s)	126, 537, 1708
MlsI	TGGCCA	1 cut(s)	623
MluNI	TGGCCA	1 cut(s)	623
MlyI	GAGTC	4 cut(s)	1009, 1898, 2218, 2750
MmeI	TCCRAC	2 cut(s)	1268, 1850
Mox20I	TGGCCA	1 cut(s)	623
Mph1103I	ATGCAT	2 cut(s)	859, 2350
MroI	TCCGGA	1 cut(s)	297
MroNI	GCCGGC	1 cut(s)	226
MroXI	GAANNNNTTC	1 cut(s)	464
MscI	TGGCCA	1 cut(s)	623
MseI	TTAA	8 cut(s)	672, 707, 1143, 1301, 1692, 1808, 1875, 2127
MslI	CAYNNNNRTG	4 cut(s)	473, 974, 1223, 2180
Msp20I	TGGCCA	1 cut(s)	623
MspA1I	CMGCKG	1 cut(s)	2723
MspI	CCGG	6 cut(s)	202, 227, 263, 298, 1766, 2601
MspR9I	CCNGG	7 cut(s)	316, 456, 1767, 1887, 2408, 2826, 2847
MunI	CAATTG	1 cut(s)	987
Mva1269I	GAATGC	3 cut(s)	373, 857, 2890
MvaI	CCWGG	6 cut(s)	316, 456, 1887, 2408, 2826, 2847
MwoI	GCNNNNNNNGC	6 cut(s)	519, 1206, 1215, 1684, 2965, 3008
NaeI	GCCGGC	1 cut(s)	228
NciI	CCSGG	1 cut(s)	1767
NcoI	CCATGG	1 cut(s)	267
NdeI	CATATG	3 cut(s)	1162, 1405, 2553
NgoMIV	GCCGGC	1 cut(s)	226
NlaIV	GGNNCC	9 cut(s)	313, 430, 848, 1183, 1516, 1859, 2274, 2428, 2451
NmuCI	GTSAC	4 cut(s)	2230, 2458, 2753, 2984
NsiI	ATGCAT	2 cut(s)	859, 2350
NspI	RCATGY	2 cut(s)	1687, 1984
NspV	TTCGAA	1 cut(s)	664
OliI	CACNNNNGTG	2 cut(s)	1223, 2180
PaeR7I	CTCGAG	1 cut(s)	119
PaqCI	CACCTGC	1 cut(s)	1959
PciI	ACATGT	1 cut(s)	1980
PciSI	GCTCTTC	2 cut(s)	2049, 2836
PcsI	WCGNNNNNNNCGW	3 cut(s)	66, 171, 476
PctI	GAATGC	3 cut(s)	373, 857, 2890
PdiI	GCCGGC	1 cut(s)	228
PdmI	GAANNNNTTC	1 cut(s)	464
PfeI	GAWTC	8 cut(s)	171, 1400, 1615, 1777, 2193, 2302, 2471, 2528
PflMI	CCANNNNNTGG	1 cut(s)	2234
PfoI	TCCNGGA	2 cut(s)	1765, 2406
PinAI	ACCGGT	1 cut(s)	2600
PkrI	GCNGC	5 cut(s)	453, 1125, 1342, 1730, 2112
PleI	GAGTC	4 cut(s)	1009, 1898, 2218, 2750
PpsI	GAGTC	4 cut(s)	1009, 1898, 2218, 2750
Ppu21I	YACGTR	1 cut(s)	2213
PpuMI	RGGWCCY	1 cut(s)	3080
PscI	ACATGT	1 cut(s)	1980
PshAI	GACNNNNGTC	2 cut(s)	2228, 2547
Psp124BI	GAGCTC	1 cut(s)	537
Psp5II	RGGWCCY	1 cut(s)	3080
Psp6I	CCWGG	6 cut(s)	314, 454, 1885, 2406, 2824, 2845
PspFI	CCCAGC	1 cut(s)	1994
PspGI	CCWGG	6 cut(s)	314, 454, 1885, 2406, 2824, 2845
PspN4I	GGNNCC	9 cut(s)	313, 430, 848, 1183, 1516, 1859, 2274, 2428, 2451
PspPPI	RGGWCCY	1 cut(s)	3080
PspXI	VCTCGAGB	1 cut(s)	119
PstNI	CAGNNNCTG	2 cut(s)	211, 2234
PsuI	RGATCY	6 cut(s)	139, 548, 1181, 2071, 2449, 2617
RseI	CAYNNNNRTG	4 cut(s)	473, 974, 1223, 2180
SacI	GAGCTC	1 cut(s)	537
SapI	GCTCTTC	2 cut(s)	2049, 2836
SaqAI	TTAA	8 cut(s)	672, 707, 1143, 1301, 1692, 1808, 1875, 2127
SatI	GCNGC	5 cut(s)	452, 1124, 1341, 1729, 2111
ScaI	AGTACT	3 cut(s)	1111, 1792, 2087
SchI	GAGTC	4 cut(s)	1009, 1898, 2218, 2750
ScrFI	CCNGG	7 cut(s)	316, 456, 1767, 1887, 2408, 2826, 2847
SduI	GDGCHC	3 cut(s)	126, 537, 1708
SfcI	CTRYAG	3 cut(s)	1069, 2724, 2947
Sfr274I	CTCGAG	1 cut(s)	119
SfuI	TTCGAA	1 cut(s)	664
SgrAI	CRCCGGYG	1 cut(s)	2600
SinI	GGWCC	7 cut(s)	295, 911, 925, 1001, 1355, 1858, 3080
SlaI	CTCGAG	1 cut(s)	119
SmiMI	CAYNNNNRTG	4 cut(s)	473, 974, 1223, 2180
SmlI	CTYRAG	3 cut(s)	119, 898, 2578
SmoI	CTYRAG	3 cut(s)	119, 898, 2578
SpeI	ACTAGT	1 cut(s)	1220
SspI	AATATT	2 cut(s)	670, 2943
SspMI	CTAG	6 cut(s)	137, 1221, 1449, 1899, 2982, 3084
SstI	GAGCTC	1 cut(s)	537
StyD4I	CCNGG	7 cut(s)	314, 454, 1765, 1885, 2406, 2824, 2845
StyI	CCWWGG	2 cut(s)	267, 1658
TaaI	ACNGT	9 cut(s)	1073, 1136, 1754, 1871, 2537, 2546, 2685, 2792, 2869
TaiI	ACGT	4 cut(s)	26, 165, 378, 2215
TaqII	GACCGA	1 cut(s)	2629
TatI	WGTACW	9 cut(s)	180, 889, 1109, 1193, 1790, 2085, 2389, 2440, 2491
TauI	GCSGC	3 cut(s)	454, 1126, 1731
TfiI	GAWTC	8 cut(s)	171, 1400, 1615, 1777, 2193, 2302, 2471, 2528
Tru1I	TTAA	8 cut(s)	672, 707, 1143, 1301, 1692, 1808, 1875, 2127
Tru9I	TTAA	8 cut(s)	672, 707, 1143, 1301, 1692, 1808, 1875, 2127
TscAI	CASTG	5 cut(s)	507, 1078, 2239, 2910, 2993
TseFI	GTSAC	4 cut(s)	2230, 2458, 2753, 2984
TseI	GCWGC	2 cut(s)	1340, 2110
Tsp45I	GTSAC	4 cut(s)	2230, 2458, 2753, 2984
TspDTI	ATGAA	5 cut(s)	34, 552, 1331, 1422, 1620
TspGWI	ACGGA	3 cut(s)	296, 510, 2265
TspRI	CASTG	5 cut(s)	507, 1078, 2239, 2910, 2993
Van91I	CCANNNNNTGG	1 cut(s)	2234
VneI	GTGCAC	1 cut(s)	1704
VpaK11BI	GGWCC	7 cut(s)	295, 911, 925, 1001, 1355, 1858, 3080
XapI	RAATTY	3 cut(s)	1960, 2704, 2860
XceI	RCATGY	2 cut(s)	1687, 1984
XhoI	CTCGAG	1 cut(s)	119
XmiI	GTMKAC	3 cut(s)	599, 1749, 2172
XmnI	GAANNNNTTC	1 cut(s)	464
XspI	CTAG	6 cut(s)	137, 1221, 1449, 1899, 2982, 3084
ZraI	GACGTC	1 cut(s)	376
ZrmI	AGTACT	3 cut(s)	1111, 1792, 2087
Zsp2I	ATGCAT	2 cut(s)	859, 2350

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.