pycom02g03800

Membrane-bound transcription factor site-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002222
Physical Location & Seq
Forward (+)
2534186 .. 2534545
360 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g03800.1

Sequence Viewer

Length: 360 bp
ATGCCTGTTATCTTTAATACTACCATTCTAAATGGGATGGGTGTAATAGGCTACGTTGAAAGTCCACCGACATGGCATCCTTCAGATGATGTAGGGAATCTTCTAAGCATTCACTTTACTTATTCTGAAGTTATTTGGCCTTGGACTGGTTATTTAGCACTTCACATGCAAATTAAGGATGAAGGGGCACAATTTTCTGGAGAGATAGGAGGCAATGTTACTCTTAGGGTATACAGTCCTCCAGCTCTAGGAGAGATGGATCGATCGGATGAGTACTTGTGTACTTCAGTTGAAATTGAAAATTGTCCCAACTCCACCAAGATCGAAACGAGTTTTGTGGGATCAGTTTCACAATATTAA

Protein Analysis

120

Amino Acids

13.15

Weight (kDa)

4.32

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBTPS1_3rd PF23094 1 - 93 2.5e-33 MBTPS1, third Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 231
AclWI GGATC 2 cut(s) 267, 349
AcuI CTGAAG 3 cut(s) 66, 147, 270
AfaI GTAC 2 cut(s) 275, 283
AfiI CCNNNNNNNGG 2 cut(s) 146, 248
AgsI TTSAA 3 cut(s) 59, 293, 299
AluBI AGCT 1 cut(s) 245
AluI AGCT 1 cut(s) 245
AlwI GGATC 2 cut(s) 267, 349
AoxI GGCC 1 cut(s) 137
BaeGI GKGCMC 1 cut(s) 190
BarI GAAGNNNNNNTAC 2 cut(s) 84, 116
BccI CCATC 2 cut(s) 31, 250
BfaI CTAG 1 cut(s) 248
BmcAI AGTACT 1 cut(s) 275
BmsI GCATC 1 cut(s) 85
BpmI CTGGAG 2 cut(s) 219, 225
Bsa29I ATCGAT 1 cut(s) 262
BsaJI CCNNGG 1 cut(s) 140
BsaXI ACNNNNNCTCC 2 cut(s) 201, 231
Bsc4I CCNNNNNNNGG 2 cut(s) 146, 248
Bse1I ACTGG 1 cut(s) 151
Bse3DI GCAATG 1 cut(s) 220
BseCI ATCGAT 1 cut(s) 262
BseDI CCNNGG 1 cut(s) 140
BseGI GGATG 4 cut(s) 42, 76, 184, 274
BseLI CCNNNNNNNGG 2 cut(s) 146, 248
BseMI GCAATG 1 cut(s) 220
BseNI ACTGG 1 cut(s) 151
BseSI GKGCMC 1 cut(s) 190
Bsh1285I CGRYCG 1 cut(s) 266
BshFI GGCC 1 cut(s) 139
BshVI ATCGAT 1 cut(s) 262
BsiEI CGRYCG 1 cut(s) 266
BslFI GGGAC 1 cut(s) 291
BslI CCNNNNNNNGG 2 cut(s) 146, 248
BsmFI GGGAC 1 cut(s) 291
BsmI GAATGC 1 cut(s) 108
BsnI GGCC 1 cut(s) 139
Bsp1286I GDGCHC 1 cut(s) 190
Bsp143I GATC 4 cut(s) 259, 263, 321, 341
BspANI GGCC 1 cut(s) 139
BspDI ATCGAT 1 cut(s) 262
BspPI GGATC 2 cut(s) 267, 349
BsrDI GCAATG 1 cut(s) 220
BsrI ACTGG 1 cut(s) 151
BssECI CCNNGG 1 cut(s) 140
BssMI GATC 4 cut(s) 259, 263, 321, 341
BssNAI GTATAC 1 cut(s) 232
BssT1I CCWWGG 1 cut(s) 140
Bst1107I GTATAC 1 cut(s) 232
Bst4CI ACNGT 1 cut(s) 236
BstDEI CTNAG 2 cut(s) 104, 224
BstF5I GGATG 4 cut(s) 42, 76, 184, 274
BstKTI GATC 4 cut(s) 262, 266, 324, 344
BstMBI GATC 4 cut(s) 259, 263, 321, 341
BstMCI CGRYCG 1 cut(s) 266
BstNSI RCATGY 1 cut(s) 169
BstSLI GKGCMC 1 cut(s) 190
BstXI CCANNNNNNTGG 1 cut(s) 72
BstZ17I GTATAC 1 cut(s) 232
Bsu15I ATCGAT 1 cut(s) 262
BsuRI GGCC 1 cut(s) 139
BsuTUI ATCGAT 1 cut(s) 262
BtsCI GGATG 4 cut(s) 42, 76, 184, 274
ClaI ATCGAT 1 cut(s) 262
Csp6I GTAC 2 cut(s) 274, 282
CviAII CATG 2 cut(s) 72, 166
CviJI RGCY 3 cut(s) 51, 139, 245
CviKI_1 RGCY 3 cut(s) 51, 139, 245
CviQI GTAC 2 cut(s) 274, 282
DdeI CTNAG 2 cut(s) 104, 224
DpnI GATC 4 cut(s) 261, 265, 323, 343
DpnII GATC 4 cut(s) 259, 263, 321, 341
Eco130I CCWWGG 1 cut(s) 140
Eco57I CTGAAG 3 cut(s) 66, 147, 270
EcoT14I CCWWGG 1 cut(s) 140
ErhI CCWWGG 1 cut(s) 140
FaeI CATG 2 cut(s) 75, 169
FaiI YATR 3 cut(s) 73, 167, 232
FaqI GGGAC 1 cut(s) 291
FatI CATG 2 cut(s) 71, 165
FblI GTMKAC 1 cut(s) 231
FokI GGATG 4 cut(s) 49, 63, 191, 281
FspBI CTAG 1 cut(s) 248
GsuI CTGGAG 2 cut(s) 219, 225
HaeIII GGCC 1 cut(s) 139
Hin1II CATG 2 cut(s) 75, 169
HinfI GANTC 1 cut(s) 97
Hpy166II GTNNAC 3 cut(s) 65, 232, 282
Hpy188I TCNGA 3 cut(s) 85, 127, 268
Hpy188III TCNNGA 1 cut(s) 198
Hpy8I GTNNAC 3 cut(s) 65, 232, 282
HpyAV CCTTC 2 cut(s) 90, 176
HpyCH4III ACNGT 1 cut(s) 236
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 1 cut(s) 169
HpyF3I CTNAG 2 cut(s) 104, 224
HpySE526I ACGT 1 cut(s) 54
Hsp92II CATG 2 cut(s) 75, 169
Kzo9I GATC 4 cut(s) 259, 263, 321, 341
LpnPI CCDG 4 cut(s) 18, 132, 183, 255
LweI GCATC 1 cut(s) 85
MaeI CTAG 1 cut(s) 248
MaeII ACGT 1 cut(s) 54
MaeIII GTNAC 1 cut(s) 217
MalI GATC 4 cut(s) 261, 265, 323, 343
MboI GATC 4 cut(s) 259, 263, 321, 341
MboII GAAGA 1 cut(s) 92
MhlI GDGCHC 1 cut(s) 190
MluCI AATT 4 cut(s) 171, 191, 294, 301
MnlI CCTC 2 cut(s) 203, 249
MseI TTAA 3 cut(s) 15, 174, 358
MslI CAYNNNNRTG 1 cut(s) 70
Mva1269I GAATGC 1 cut(s) 108
NdeII GATC 4 cut(s) 259, 263, 321, 341
NlaIII CATG 2 cut(s) 75, 169
NspI RCATGY 1 cut(s) 169
PctI GAATGC 1 cut(s) 108
PfeI GAWTC 1 cut(s) 97
Ple19I CGATCG 1 cut(s) 266
PvuI CGATCG 1 cut(s) 266
RsaI GTAC 2 cut(s) 275, 283
RsaNI GTAC 2 cut(s) 274, 282
RseI CAYNNNNRTG 1 cut(s) 70
SaqAI TTAA 3 cut(s) 15, 174, 358
Sau3AI GATC 4 cut(s) 259, 263, 321, 341
ScaI AGTACT 1 cut(s) 275
SduI GDGCHC 1 cut(s) 190
SetI ASST 2 cut(s) 57, 247
SfaNI GCATC 1 cut(s) 85
SmiMI CAYNNNNRTG 1 cut(s) 70
Sse9I AATT 4 cut(s) 171, 191, 294, 301
SspI AATATT 1 cut(s) 356
SspMI CTAG 1 cut(s) 248
StyI CCWWGG 1 cut(s) 140
TaaI ACNGT 1 cut(s) 236
TaiI ACGT 1 cut(s) 57
TaqI TCGA 2 cut(s) 262, 324
TasI AATT 4 cut(s) 171, 191, 294, 301
TatI WGTACW 2 cut(s) 273, 281
TfiI GAWTC 1 cut(s) 97
Tru1I TTAA 3 cut(s) 15, 174, 358
Tru9I TTAA 3 cut(s) 15, 174, 358
TspDTI ATGAA 1 cut(s) 195
XceI RCATGY 1 cut(s) 169
XmiI GTMKAC 1 cut(s) 231
XspI CTAG 1 cut(s) 248
ZrmI AGTACT 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.