Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0002207

Physical Location & Seq

Genomic Coordinates Forward (+)

6598414 .. 6602899

Gene Structure 4486 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1084100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2271 bp

ATGTCGTCCGTGGCGGTGGCGGTTAACAGCGGCGGGAGCCCGGGTAGTCGACGTGCCGTGAATTGGGCTGCGGAGAATCTCTTGCCCACGGCAGATCGCTTCGTTTTAGTCCACGTCATCCCCAAAATCAACTCAATCCCAACTCCATCAGGAGATCACATCCCGATCACAGAGCTGGATGCAAATTTGGTGGCAACGTATGTGAAGGACCAGAAACAAAAGTTTGAGGAAGTTTTTATTCCGTTTAAGAAGAAATGCAAAAATAATAAGAAGGTGGAAACATTGGTGTTAGAGGATGATGAACCTGCAAATGGGATTCTAAGGTTCATATCTGAATCGGGGATCGATTGTTTAGTGCTCGGCTCCTACTCTTCAAATTACGTTACCAGGAAACTGAAAGGACAGGGTGTATCACCAATTGTGCTGAGAGGTGCTCCCGACACTTGTGAAGTTTATTCCATTTCCAAACATAGAGTCATCACAAAATCAGATATTCCTTCTTCTGCTTGTGAGACTAGATCTGCTGGCCAGATGTCTACTCTAAAAAACCGAAAGGGAAGTTCAAGTGATATCAATAAACAGATAAGTGGGGTTCATTCTTCCTATGTAGAAACCAGATCTCATGAAGCTTATGGAGCATCTTCAATGTCAGACCTTAGTTACCTAAGTTCTGAAGCATTCACGCGTATGAGAATTTCCTCAAATGTTAGTGTAGATCAGGAAAGAAATCATCAAAACTTGGGAGATAACCTGGATATTAGTTCTTTTAAGGATCGAAGTTCCTCGGGTTCTTCAATTACTGAGCTGTCACATGTGCAGATGCAGGTAGAACAGTTGCGTCTGGAATTGCAAAATACTATTGCCATGTACAAACAAGCTTGTGAAGAGCTGGTCCATGCCCAAAGTAAGGTGCAATTACTTTCTTCCGAATGCCTTGATGAAACAAGAAGAGTGAATGCTGCCCTTGAAAGTGAAGAAATTTTGAGAAAAATTGCTGCGGAAGAGAAAGCAAAGCATTTGGAGGCACTGAAGGAGATTGAGGAGGCCAAGGTTCTGCTTGCTAAAGAGGCTTATGAGAGGCAAATGGCTGAGCTGACTGCCATGAAAGAGTCCTCAGAGAAAAATAAATTAGTTGATGCACTCTTCTCAAGTGACAGGAGGTATAGGAGATACAGTAGGAATGAAATAGAGGTAGCAACGAGTTTCTTCTCAGAGGCTAATGTGATTGGTGAAGGAGGATATGGAAAAGTTTACAAATGCAGTCTTGATCGCACCCCGGTAGCTGTTAAGGTTCTTCGACCTGATGCAGTTGATAAGAAAGAGGAGTTTCTGAAAGAGGTTGAAATTCTTAGTCAGCTACACCATCCGAACATAGTTTTGCTGCTTGGTGCTTGTCCTGAGATTGGTTGCCTGGTCTATGAATACTTGGAAAATGGAAGTTTGGAAGATTATGTCTACTGCCAAAATGGAAAACCGCCACTCCCTTGGACTATTCGGTTTCGTATAGTTTTTGAAGTAGCTTGTGGGCTTGCATTCTTACACAACTCTAAGCTGGATCCTATTGTGCATCGAGATCTAAAGCCGGGAAACATCTTATTAGACAGAAACTACATGAGCAAAATAGGGGATGTTGGGCTGGCTAAACTCATTTCAAACGTCGTGCCTGACAACATCACTGAGTACAGAGAATCAATTCTCGCTGGTACATTCTTTTATATGGATCCAGAGTATCAAAGAACTGGCACTGTCCGGCCCAAATCAGATTTGTATGCATTTGGTGTCATAATCCTTCAAGTACTGACAGGTTGCTATCCAAATCGGCTTATATTTGTTACGGAGAATGCAATTGCAAATGGGTCATTTGCTGAAACCTTGGACAAGTCGGTTGCAGATTGGCCACTTGCAGAAGCAGAGGAGTTGGCTCGAGTTGCACTATGGTGTTCACAACTTAGATGCAGGGATAGACCAGATCTTGAAACTGAAGTTTTGCCGGTTCTCAAACGACTCGCTAATCTTGCAGATTCTAGCTTGAAGTTTGATAGAAACCATATCAATGCACCAAACCACTTCTTTTGTCCAATACTTCAGGAAATCATGGAGGATCCACACGTAGCTGCAGACGGTTTTACATATGAGTATCGAGCGATCAAAGCATGGATTAAGAAACACAAAGTGTCCCCGTTGACAAGGCTTAGACTAAAGAATTTTGAGTTGACTCCAAACCACACATTGCGTTCTGCCATACAGGAATGGAGGTCAGGTTTGACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr U-box Usp

Protein Analysis

757

Amino Acids

84.81

Weight (kDa)

6.36

Isoelectric Point (pI)

48.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Usp	PF00582	4 - 132	9.7e-10	Universal stress protein family
Pkinase	PF00069	404 - 609	1.1e-37	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	407 - 659	2.4e-39	Protein tyrosine and serine/threonine kinase
U-box	PF04564	686 - 752	1.3e-16	U-box domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002207)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19410 AT2G19410 AT2G19410 AT5G57035
fragaria_vesca	FvH4_1g07970 FvH4_1g07970 FvH4_1g07970 FvH4_1g07970 FvH4_1g07970
malus_domestica	MD02G1084100.v1.1
prunus_persica	Prupe.7G205400_v2.0.a1 Prupe.7G205400_v2.0.a1 Prupe.7G205400_v2.0.a1 Prupe.7G205400_v2.0.a1 Prupe.7G205400_v2.0.a1 Prupe.7G205400_v2.0.a1 Prupe.7G205400_v2.0.a1 Prupe.7G205400_v2.0.a1
pyrus_communis	pycom02g06680 pycom02g06690
rosa_chinensis	RchiOBHm_Chr1g0330911 RchiOBHm_Chr2g0093881 RchiOBHm_Chr2g0094001 RchiOBHm_Chr7g0232521
rosa_laevigata	RLG00000001329 RLG00000016442 RLG00000016444
rosa_multiflora	Rmu_sc0003027.1_g000005 Rmu_sc0017523.1_g000016 Rmu_ssc0000369.1_g000008
rosa_roxburghii	Rroxscaffold_2G00147570 Rroxscaffold_2G00147700
rosa_rugosa	Rorug02G0040200 Rorug02G0040300
rosa_samantha	Rh2AG086900 Rh2AG087700 Rh2BG087700 Rh2CG090000 Rh2DG085600 Rh2DG086700 Rh2DG086800 Rh3CG371600 Rh7CG448000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	313, 814
AccI	GTMKAC	3 cut(s)	49, 536, 1455
AccII	CGCG	1 cut(s)	685
AciI	CCGC	7 cut(s)	14, 20, 30, 33, 71, 998, 1475
AclWI	GGATC	8 cut(s)	350, 780, 1550, 1563, 1715, 1728, 2096, 2109
AcoI	YGGCCR	2 cut(s)	526, 1895
AcsI	RAATTY	5 cut(s)	184, 693, 978, 1344, 2203
AcuI	CTGAAG	4 cut(s)	693, 1049, 2001, 2069
AdeI	CACNNNGTG	1 cut(s)	2173
AfaI	GTAC	4 cut(s)	869, 1682, 1705, 1797
AfiI	CCNNNNNNNGG	4 cut(s)	63, 311, 907, 1403
AflIII	ACRYGT	3 cut(s)	683, 811, 2107
AjiI	CACGTC	2 cut(s)	53, 115
AjnI	CCWGG	3 cut(s)	386, 750, 1410
AleI	CACNNNNGTG	1 cut(s)	1936
Alw21I	GWGCWC	2 cut(s)	360, 436
Alw26I	GTCTC	1 cut(s)	506
AlwI	GGATC	8 cut(s)	350, 780, 1550, 1563, 1715, 1728, 2096, 2109
Ama87I	CYCGRG	3 cut(s)	40, 784, 1923
AoxI	GGCC	4 cut(s)	526, 1044, 1751, 1895
ApeKI	GCWGC	5 cut(s)	68, 959, 995, 1381, 2114
ApoI	RAATTY	5 cut(s)	184, 693, 978, 1344, 2203
ArsI	GACNNNNNNTTYG	2 cut(s)	459, 491
Asp700I	GAANNNNTTC	1 cut(s)	1692
AspS9I	GGNCC	3 cut(s)	208, 892, 1752
AsuC2I	CCSGG	4 cut(s)	41, 42, 1277, 1584
AsuHPI	GGTGA	2 cut(s)	405, 1241
AvaI	CYCGRG	3 cut(s)	40, 784, 1923
AvaII	GGWCC	2 cut(s)	208, 892
BalI	TGGCCA	2 cut(s)	528, 1897
BamHI	GGATCC	3 cut(s)	1555, 1720, 2101
BanII	GRGCYC	1 cut(s)	41
Bbv12I	GWGCWC	2 cut(s)	360, 436
BbvI	GCAGC	5 cut(s)	55, 946, 982, 1368, 2101
BccI	CCATC	2 cut(s)	154, 1371
BceAI	ACGGC	2 cut(s)	41, 105
BciT130I	CCWGG	3 cut(s)	388, 752, 1412
BcnI	CCSGG	4 cut(s)	41, 42, 1277, 1584
BcoDI	GTCTC	1 cut(s)	506
BfaI	CTAG	2 cut(s)	516, 2025
BfmI	CTRYAG	1 cut(s)	2115
BfuAI	ACCTGC	2 cut(s)	313, 814
BglII	AGATCT	4 cut(s)	518, 617, 1573, 1969
BisI	GCNGC	6 cut(s)	31, 69, 960, 996, 1382, 2115
BlpI	GCTNAGC	1 cut(s)	1089
BlsI	GCNGC	6 cut(s)	32, 70, 961, 997, 1383, 2116
BmcAI	AGTACT	1 cut(s)	1797
Bme1390I	CCNGG	7 cut(s)	41, 42, 388, 752, 1277, 1412, 1584
Bme18I	GGWCC	2 cut(s)	208, 892
BmeT110I	CYCGRG	3 cut(s)	40, 784, 1923
BmgBI	CACGTC	2 cut(s)	53, 115
BmgT120I	GGNCC	3 cut(s)	208, 892, 1752
BmiI	GGNNCC	5 cut(s)	38, 364, 1557, 1722, 2103
BmrFI	CCNGG	7 cut(s)	41, 42, 388, 752, 1277, 1412, 1584
BmsI	GCATC	7 cut(s)	169, 647, 810, 1126, 1294, 1576, 1943
BplI	GAGNNNNNCTC	2 cut(s)	418, 450
Bpu1102I	GCTNAGC	1 cut(s)	1089
BpuEI	CTTGAG	1 cut(s)	1132
BpuMI	CCSGG	4 cut(s)	41, 42, 1277, 1584
Bsa29I	ATCGAT	1 cut(s)	345
BsaAI	YACGTR	1 cut(s)	2110
BsaJI	CCNNGG	8 cut(s)	9, 40, 87, 783, 1047, 1275, 1484, 1872
BsaXI	ACNNNNNCTCC	7 cut(s)	28, 58, 1159, 1189, 1464, 1494, 2245
Bsc4I	CCNNNNNNNGG	4 cut(s)	63, 311, 907, 1403
Bse118I	RCCGGY	1 cut(s)	1990
Bse1I	ACTGG	1 cut(s)	1744
Bse3DI	GCAATG	1 cut(s)	2228
BseBI	CCWGG	3 cut(s)	388, 752, 1412
BseCI	ATCGAT	1 cut(s)	345
BseDI	CCNNGG	8 cut(s)	9, 40, 87, 783, 1047, 1275, 1484, 1872
BseGI	GGATG	6 cut(s)	117, 159, 184, 301, 1363, 1633
BseLI	CCNNNNNNNGG	4 cut(s)	63, 311, 907, 1403
BseMI	GCAATG	1 cut(s)	2228
BseMII	CTCAG	7 cut(s)	416, 792, 1080, 1128, 1224, 1389, 1668
BseNI	ACTGG	1 cut(s)	1744
BseRI	GAGGAG	3 cut(s)	1055, 1337, 1928
BseXI	GCAGC	5 cut(s)	55, 946, 982, 1368, 2101
BsgI	GTGCAG	1 cut(s)	836
Bsh1236I	CGCG	1 cut(s)	685
BshFI	GGCC	4 cut(s)	528, 1046, 1753, 1897
BshVI	ATCGAT	1 cut(s)	345
BsiHKAI	GWGCWC	2 cut(s)	360, 436
BsiHKCI	CYCGRG	3 cut(s)	40, 784, 1923
BsiSI	CCGG	5 cut(s)	41, 1277, 1583, 1750, 1991
BslFI	GGGAC	1 cut(s)	2161
BslI	CCNNNNNNNGG	4 cut(s)	63, 311, 907, 1403
BsmAI	GTCTC	1 cut(s)	506
BsmFI	GGGAC	1 cut(s)	2161
BsmI	GAATGC	5 cut(s)	677, 935, 961, 1532, 1846
BsnI	GGCC	4 cut(s)	528, 1046, 1753, 1897
BsoBI	CYCGRG	3 cut(s)	40, 784, 1923
Bsp1286I	GDGCHC	3 cut(s)	41, 360, 436
Bsp1407I	TGTACA	1 cut(s)	867
Bsp1720I	GCTNAGC	1 cut(s)	1089
BspACI	CCGC	7 cut(s)	14, 20, 30, 33, 71, 998, 1475
BspANI	GGCC	4 cut(s)	528, 1046, 1753, 1897
BspCNI	CTCAG	7 cut(s)	417, 793, 1081, 1127, 1223, 1390, 1669
BspDI	ATCGAT	1 cut(s)	345
BspFNI	CGCG	1 cut(s)	685
BspHI	TCATGA	1 cut(s)	622
BspLI	GGNNCC	5 cut(s)	38, 364, 1557, 1722, 2103
BspMAI	CTGCAG	1 cut(s)	2119
BspMI	ACCTGC	2 cut(s)	313, 814
BspPI	GGATC	8 cut(s)	350, 780, 1550, 1563, 1715, 1728, 2096, 2109
BspQI	GCTCTTC	1 cut(s)	879
BsrDI	GCAATG	1 cut(s)	2228
BsrFI	RCCGGY	1 cut(s)	1990
BsrGI	TGTACA	1 cut(s)	867
BsrI	ACTGG	1 cut(s)	1744
BssAI	RCCGGY	1 cut(s)	1990
BssECI	CCNNGG	8 cut(s)	9, 40, 87, 783, 1047, 1275, 1484, 1872
BssT1I	CCWWGG	3 cut(s)	1047, 1484, 1872
Bst2UI	CCWGG	3 cut(s)	388, 752, 1412
Bst4CI	ACNGT	4 cut(s)	834, 1175, 1747, 2123
Bst6I	CTCTTC	5 cut(s)	376, 879, 943, 996, 1148
BstAUI	TGTACA	1 cut(s)	867
BstBAI	YACGTR	1 cut(s)	2110
BstC8I	GCNNGC	4 cut(s)	526, 1059, 1530, 1638
BstDSI	CCRYGG	2 cut(s)	9, 87
BstF5I	GGATG	6 cut(s)	117, 159, 184, 301, 1363, 1633
BstFNI	CGCG	1 cut(s)	685
BstMAI	GTCTC	1 cut(s)	506
BstMWI	GCNNNNNNNGC	6 cut(s)	36, 635, 1067, 1928, 2015, 2150
BstNI	CCWGG	3 cut(s)	388, 752, 1412
BstNSI	RCATGY	1 cut(s)	815
BstSCI	CCNGG	7 cut(s)	39, 40, 386, 750, 1275, 1410, 1582
BstSFI	CTRYAG	1 cut(s)	2115
BstUI	CGCG	1 cut(s)	685
BstV1I	GCAGC	5 cut(s)	55, 946, 982, 1368, 2101
BstX2I	RGATCY	7 cut(s)	518, 617, 1555, 1573, 1720, 1969, 2101
BstXI	CCANNNNNNTGG	1 cut(s)	1485
BstYI	RGATCY	7 cut(s)	518, 617, 1555, 1573, 1720, 1969, 2101
Bsu15I	ATCGAT	1 cut(s)	345
BsuRI	GGCC	4 cut(s)	528, 1046, 1753, 1897
BsuTUI	ATCGAT	1 cut(s)	345
BtgI	CCRYGG	2 cut(s)	9, 87
BtrI	CACGTC	2 cut(s)	53, 115
BtsCI	GGATG	6 cut(s)	117, 159, 184, 301, 1363, 1633
BtsIMutI	CAGTG	3 cut(s)	1025, 1674, 1743
BveI	ACCTGC	2 cut(s)	313, 814
Cac8I	GCNNGC	4 cut(s)	526, 1059, 1530, 1638
CciI	TCATGA	1 cut(s)	622
Cfr10I	RCCGGY	1 cut(s)	1990
Cfr13I	GGNCC	3 cut(s)	208, 892, 1752
Cfr9I	CCCGGG	1 cut(s)	40
ClaI	ATCGAT	1 cut(s)	345
CseI	GACGC	1 cut(s)	827
Csp6I	GTAC	4 cut(s)	868, 1681, 1704, 1796
CspCI	CAANNNNNGTGG	4 cut(s)	171, 206, 2054, 2089
CviAII	CATG	8 cut(s)	623, 812, 865, 896, 1102, 1612, 2095, 2154
CviQI	GTAC	4 cut(s)	868, 1681, 1704, 1796
DraIII	CACNNNGTG	1 cut(s)	2173
EaeI	YGGCCR	2 cut(s)	526, 1895
Eam1104I	CTCTTC	5 cut(s)	376, 879, 943, 996, 1148
EarI	CTCTTC	5 cut(s)	376, 879, 943, 996, 1148
Eco130I	CCWWGG	3 cut(s)	1047, 1484, 1872
Eco24I	GRGCYC	1 cut(s)	41
Eco32I	GATATC	1 cut(s)	571
Eco47I	GGWCC	2 cut(s)	208, 892
Eco57I	CTGAAG	4 cut(s)	693, 1049, 2001, 2069
Eco88I	CYCGRG	3 cut(s)	40, 784, 1923
EcoRII	CCWGG	3 cut(s)	386, 750, 1410
EcoRV	GATATC	1 cut(s)	571
EcoT14I	CCWWGG	3 cut(s)	1047, 1484, 1872
EcoT22I	ATGCAT	1 cut(s)	1774
EcoT38I	GRGCYC	1 cut(s)	41
ErhI	CCWWGG	3 cut(s)	1047, 1484, 1872
FaeI	CATG	8 cut(s)	626, 815, 868, 899, 1105, 1615, 2098, 2157
FaqI	GGGAC	1 cut(s)	2161
FatI	CATG	8 cut(s)	622, 811, 864, 895, 1101, 1611, 2094, 2153
FauI	CCCGC	1 cut(s)	26
FauNDI	CATATG	1 cut(s)	2131
FblI	GTMKAC	3 cut(s)	49, 536, 1455
Fnu4HI	GCNGC	6 cut(s)	31, 69, 960, 996, 1382, 2115
FokI	GGATG	6 cut(s)	104, 146, 191, 308, 1350, 1640
FriOI	GRGCYC	1 cut(s)	41
Fsp4HI	GCNGC	6 cut(s)	31, 69, 960, 996, 1382, 2115
FspBI	CTAG	2 cut(s)	516, 2025
GluI	GCNGC	6 cut(s)	31, 69, 960, 996, 1382, 2115
HaeIII	GGCC	4 cut(s)	528, 1046, 1753, 1897
HapII	CCGG	5 cut(s)	41, 1277, 1583, 1750, 1991
HgaI	GACGC	1 cut(s)	827
Hin1II	CATG	8 cut(s)	626, 815, 868, 899, 1105, 1615, 2098, 2157
HincII	GTYRAC	4 cut(s)	25, 50, 2184, 2214
HindII	GTYRAC	4 cut(s)	25, 50, 2184, 2214
HindIII	AAGCTT	2 cut(s)	627, 876
HinfI	GANTC	9 cut(s)	76, 316, 335, 474, 1109, 1688, 2004, 2021, 2215
HpaI	GTTAAC	1 cut(s)	25
HpaII	CCGG	5 cut(s)	41, 1277, 1583, 1750, 1991
HphI	GGTGA	2 cut(s)	405, 1241
Hpy166II	GTNNAC	9 cut(s)	25, 50, 112, 537, 1252, 1456, 1943, 2184, 2214
Hpy8I	GTNNAC	9 cut(s)	25, 50, 112, 537, 1252, 1456, 1943, 2184, 2214
Hpy99I	CGWCG	2 cut(s)	54, 1661
HpyAV	CCTTC	6 cut(s)	199, 265, 507, 1024, 1226, 1799
HpyCH4III	ACNGT	4 cut(s)	834, 1175, 1747, 2123
HpyCH4IV	ACGT	6 cut(s)	52, 114, 197, 381, 1656, 2109
HpyF10VI	GCNNNNNNNGC	6 cut(s)	36, 635, 1067, 1928, 2015, 2150
HpySE526I	ACGT	6 cut(s)	52, 114, 197, 381, 1656, 2109
Hsp92II	CATG	8 cut(s)	626, 815, 868, 899, 1105, 1615, 2098, 2157
KspAI	GTTAAC	1 cut(s)	25
LguI	GCTCTTC	1 cut(s)	879
LmnI	GCTCC	4 cut(s)	36, 368, 439, 635
Lsp1109I	GCAGC	5 cut(s)	55, 946, 982, 1368, 2101
LweI	GCATC	7 cut(s)	169, 647, 810, 1126, 1294, 1576, 1943
MaeI	CTAG	2 cut(s)	516, 2025
MaeII	ACGT	6 cut(s)	52, 114, 197, 381, 1656, 2109
MaeIII	GTNAC	5 cut(s)	382, 659, 807, 1151, 1831
MfeI	CAATTG	2 cut(s)	417, 1845
MflI	RGATCY	7 cut(s)	518, 617, 1555, 1573, 1720, 1969, 2101
MhlI	GDGCHC	3 cut(s)	41, 360, 436
MlsI	TGGCCA	2 cut(s)	528, 1897
MluI	ACGCGT	1 cut(s)	683
MluNI	TGGCCA	2 cut(s)	528, 1897
MlyI	GAGTC	4 cut(s)	483, 1118, 1998, 2209
Mox20I	TGGCCA	2 cut(s)	528, 1897
Mph1103I	ATGCAT	1 cut(s)	1774
MroXI	GAANNNNTTC	1 cut(s)	1692
MscI	TGGCCA	2 cut(s)	528, 1897
MseI	TTAA	6 cut(s)	24, 246, 768, 1287, 2160, 2269
MslI	CAYNNNNRTG	3 cut(s)	686, 1936, 2052
Msp20I	TGGCCA	2 cut(s)	528, 1897
MspA1I	CMGCKG	1 cut(s)	30
MspI	CCGG	5 cut(s)	41, 1277, 1583, 1750, 1991
MspR9I	CCNGG	7 cut(s)	41, 42, 388, 752, 1277, 1412, 1584
MunI	CAATTG	2 cut(s)	417, 1845
Mva1269I	GAATGC	5 cut(s)	677, 935, 961, 1532, 1846
MvaI	CCWGG	3 cut(s)	388, 752, 1412
MvnI	CGCG	1 cut(s)	685
MwoI	GCNNNNNNNGC	6 cut(s)	36, 635, 1067, 1928, 2015, 2150
NciI	CCSGG	4 cut(s)	41, 42, 1277, 1584
NdeI	CATATG	1 cut(s)	2131
NlaIII	CATG	8 cut(s)	626, 815, 868, 899, 1105, 1615, 2098, 2157
NlaIV	GGNNCC	5 cut(s)	38, 364, 1557, 1722, 2103
NmeAIII	GCCGAG	1 cut(s)	339
NmuCI	GTSAC	2 cut(s)	807, 1151
NsiI	ATGCAT	1 cut(s)	1774
NspI	RCATGY	1 cut(s)	815
OliI	CACNNNNGTG	1 cut(s)	1936
PaeR7I	CTCGAG	1 cut(s)	1923
PagI	TCATGA	1 cut(s)	622
PciI	ACATGT	1 cut(s)	811
PciSI	GCTCTTC	1 cut(s)	879
PctI	GAATGC	5 cut(s)	677, 935, 961, 1532, 1846
PdmI	GAANNNNTTC	1 cut(s)	1692
PfeI	GAWTC	5 cut(s)	76, 316, 335, 1688, 2021
PkrI	GCNGC	6 cut(s)	32, 70, 961, 997, 1383, 2116
PleI	GAGTC	4 cut(s)	482, 1117, 1998, 2209
PpsI	GAGTC	4 cut(s)	482, 1117, 1998, 2209
Ppu21I	YACGTR	1 cut(s)	2110
PscI	ACATGT	1 cut(s)	811
Psp6I	CCWGG	3 cut(s)	386, 750, 1410
PspGI	CCWGG	3 cut(s)	386, 750, 1410
PspN4I	GGNNCC	5 cut(s)	38, 364, 1557, 1722, 2103
PspPI	GGNCC	3 cut(s)	208, 892, 1752
PspXI	VCTCGAGB	1 cut(s)	1923
PstI	CTGCAG	1 cut(s)	2119
PsuI	RGATCY	7 cut(s)	518, 617, 1555, 1573, 1720, 1969, 2101
RsaI	GTAC	4 cut(s)	869, 1682, 1705, 1797
RsaNI	GTAC	4 cut(s)	868, 1681, 1704, 1796
RseI	CAYNNNNRTG	3 cut(s)	686, 1936, 2052
SalI	GTCGAC	1 cut(s)	48
SapI	GCTCTTC	1 cut(s)	879
SaqAI	TTAA	6 cut(s)	24, 246, 768, 1287, 2160, 2269
SatI	GCNGC	6 cut(s)	31, 69, 960, 996, 1382, 2115
Sau96I	GGNCC	3 cut(s)	208, 892, 1752
ScaI	AGTACT	1 cut(s)	1797
SchI	GAGTC	4 cut(s)	483, 1118, 1998, 2209
ScrFI	CCNGG	7 cut(s)	41, 42, 388, 752, 1277, 1412, 1584
SduI	GDGCHC	3 cut(s)	41, 360, 436
SfaNI	GCATC	7 cut(s)	169, 647, 810, 1126, 1294, 1576, 1943
SfcI	CTRYAG	1 cut(s)	2115
Sfr274I	CTCGAG	1 cut(s)	1923
SinI	GGWCC	2 cut(s)	208, 892
SlaI	CTCGAG	1 cut(s)	1923
SmaI	CCCGGG	1 cut(s)	42
SmiMI	CAYNNNNRTG	3 cut(s)	686, 1936, 2052
SmlI	CTYRAG	2 cut(s)	1147, 1923
SmoI	CTYRAG	2 cut(s)	1147, 1923
SsiI	CCGC	7 cut(s)	14, 20, 30, 33, 71, 998, 1475
SspMI	CTAG	2 cut(s)	516, 2025
StyD4I	CCNGG	7 cut(s)	39, 40, 386, 750, 1275, 1410, 1582
StyI	CCWWGG	3 cut(s)	1047, 1484, 1872
TaaI	ACNGT	4 cut(s)	834, 1175, 1747, 2123
TaiI	ACGT	6 cut(s)	55, 117, 200, 384, 1659, 2112
TaqI	TCGA	7 cut(s)	49, 345, 775, 1297, 1570, 1924, 2140
TatI	WGTACW	3 cut(s)	867, 1680, 1795
TauI	GCSGC	1 cut(s)	33
TfiI	GAWTC	5 cut(s)	76, 316, 335, 1688, 2021
Tru1I	TTAA	6 cut(s)	24, 246, 768, 1287, 2160, 2269
Tru9I	TTAA	6 cut(s)	24, 246, 768, 1287, 2160, 2269
TscAI	CASTG	3 cut(s)	1032, 1681, 1750
TseFI	GTSAC	2 cut(s)	807, 1151
TseI	GCWGC	5 cut(s)	68, 959, 995, 1381, 2114
Tsp45I	GTSAC	2 cut(s)	807, 1151
TspDTI	ATGAA	8 cut(s)	315, 316, 584, 639, 954, 1118, 1197, 1434
TspGWI	ACGGA	2 cut(s)	231, 1850
TspMI	CCCGGG	1 cut(s)	40
TspRI	CASTG	3 cut(s)	1032, 1681, 1750
VpaK11BI	GGWCC	2 cut(s)	208, 892
XapI	RAATTY	5 cut(s)	184, 693, 978, 1344, 2203
XceI	RCATGY	1 cut(s)	815
XhoI	CTCGAG	1 cut(s)	1923
XmaI	CCCGGG	1 cut(s)	40
XmiI	GTMKAC	3 cut(s)	49, 536, 1455
XmnI	GAANNNNTTC	1 cut(s)	1692
XspI	CTAG	2 cut(s)	516, 2025
ZrmI	AGTACT	1 cut(s)	1797
Zsp2I	ATGCAT	1 cut(s)	1774

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.