Rh2DG086700

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0002207
Physical Location & Seq
Forward (+)
6724745 .. 6735899
11155 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG086700.1

Sequence Viewer

Length: 1593 bp
ATGACTTGTCTGAAGGGACCAGATGTACCGACAGTTGTTCTGAAATGTGCTCCCGAGAGTTGTGATATTCATGTCGTTTCGAAACAAAGAGTTATTACAAAATCAGCTAGTTCCTCATCTGCTTGTGAGTCTAGTACTGCTAACGGGCTGTCTACTCGAGGAGAAGATGGGGGGTTTTCCTCAGATATGAATGAAGGGATAACTGGAAGTCGTTCTTCCTACCTAGTATCCACAGTTAATGAAGGTTATGGAGCACCGTCAATGTCAGATCTTAGTTATATAACTTCTGAAGCATTTACACGTATGCAGTGTCCTGCAAATGCTAGTGTGGATCAGGAAAGGAATCATCAGAACTTGGAAGAGAACCTTCAGATTATTTCTTTCCAGCGTGGAAGTTCCTCAATTTCTTCATACATTGAGCAGATTGAAGTGGAAAAGTTGAGTGCAGAATTGCAAAATACTCTTGGCATGTACAAACGAGCTTGTGAAGAGCTGGTACATGCCCAAAGCAAGGTCCAACTACTTTCTTCCGAATTCCTTGAAGAAGCAAGAAGAGTGAATGCTGCTCTTGAGAGTGAGGAAACTTTGAGAAAAATTGCTGCTGAAGAGGCAGCAAAGCATTTGAAGGCCAGGAAAGAGATTGAGGAGGCAAAAGTTCTGCTTGCTAAAGAGGCTTATGAGAGGCAAGTTGCTGAATTAAATGCCCTGAAAAAGTCGACAGGGAAAAAGGAATTTGTTGATGCACTCTTCTCATGTGACAATAGGTACAGGAGATACTGTAGGAAGGGAATAGAGATAGCAACAAACTTCTTCTTAGAGACTAATGTGATTGGTGAAGGAGGATATGGGAAAGTTTACAAGTGCAGTCTTGATCAAACCCCGGTAGTTGTTAAGGTTCTTGGACCTGATGCAGTTGAGAAGGAGGAGTTTCTGAAAGAGGTTGAAATTCTTAGTCAGCTACATCATCCTAACATAGTTTTGCTTCTTGGAGCCTGTCCAGAGATCGCGTGTGGGCTTGCATTCTTACACAACTCAAAGACAGACCCTATTGTGCATCGAGATCTAAAACCAGGTAATGTCTTACTGGACAGAAACTACTCGAGCAAAATTGGGGACGTTGGGCTGGCTAAACTCATTACAGACATCGTGCCTGACAACATAACGGAGTACAGAGAATCAATTATTGCTGGTACACTCTTTTACATGGATCCAGAGTATCAGAGAACTGGCACAATTAGACCCAAATCGGATTTATACTCATTTGGGGTCATTATCCTCCAAGTATTGACAGCACGGCATCCAAATAGGCTTATATACATTGTGGAAAATGCACTTGCAAATGGTTCTTTTGCCGACTTTTTAGATGAGTCAGTCACAGATTGGCCACTAGAAGAAACAGAGGAGTTGGCTCAACTTGCATTAAGGTGTTCAAAGCTTAGATGCAGGGATAGATCGGACCTTGAAACTGAAGTTCTGCCAGTTCTCAAAAGACTCCTTGATATTGCAGATCCTAGTTCTAAGATTGAGAGAAACCATATTGATGCACCAAGCCACTACTATTGTCCAATACTTCAGGTAAAGTATTGTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr U-box Usp

Protein Analysis

530

Amino Acids

58.99

Weight (kDa)

5.31

Isoelectric Point (pI)

50.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 274 - 332 6.2e-08 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 275 - 332 1.8e-10 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 334 - 484 1.1e-18 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 334 - 488 8.8e-16 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 152, 716
AccII CGCG 1 cut(s) 1007
AclWI GGATC 4 cut(s) 339, 1202, 1215, 1502
AcoI YGGCCR 1 cut(s) 1382
AcsI RAATTY 3 cut(s) 533, 731, 945
AcuI CTGAAG 6 cut(s) 32, 309, 353, 624, 1488, 1556
AfaI GTAC 7 cut(s) 27, 136, 473, 498, 767, 1169, 1192
AfiI CCNNNNNNNGG 1 cut(s) 511
AflIII ACRYGT 1 cut(s) 299
AgsI TTSAA 6 cut(s) 428, 542, 625, 944, 1431, 1463
AjnI CCWGG 2 cut(s) 629, 1069
AluBI AGCT 5 cut(s) 107, 482, 493, 958, 1435
AluI AGCT 5 cut(s) 107, 482, 493, 958, 1435
Alw21I GWGCWC 2 cut(s) 52, 256
Alw26I GTCTC 1 cut(s) 812
AlwI GGATC 4 cut(s) 339, 1202, 1215, 1502
Ama87I CYCGRG 3 cut(s) 53, 156, 1099
AoxI GGCC 2 cut(s) 627, 1382
ApeKI GCWGC 3 cut(s) 563, 599, 611
ApoI RAATTY 3 cut(s) 533, 731, 945
Asp700I GAANNNNTTC 1 cut(s) 211
AspS9I GGNCC 4 cut(s) 17, 514, 902, 1456
AsuC2I CCSGG 1 cut(s) 881
AsuHPI GGTGA 1 cut(s) 845
AsuII TTCGAA 1 cut(s) 80
AvaI CYCGRG 3 cut(s) 53, 156, 1099
AvaII GGWCC 4 cut(s) 17, 514, 902, 1456
BalI TGGCCA 1 cut(s) 1384
BamHI GGATCC 1 cut(s) 1207
BarI GAAGNNNNNNTAC 2 cut(s) 480, 512
Bbv12I GWGCWC 2 cut(s) 52, 256
BbvI GCAGC 3 cut(s) 550, 586, 623
BccI CCATC 1 cut(s) 161
BceAI ACGGC 1 cut(s) 1310
BciT130I CCWGG 2 cut(s) 631, 1071
BciVI GTATCC 1 cut(s) 238
BclI TGATCA 1 cut(s) 871
BcnI CCSGG 1 cut(s) 881
BcoDI GTCTC 1 cut(s) 812
BfaI CTAG 6 cut(s) 108, 132, 224, 324, 1388, 1512
BfmI CTRYAG 1 cut(s) 778
BfuI GTATCC 1 cut(s) 238
BglII AGATCT 2 cut(s) 268, 1060
BisI GCNGC 3 cut(s) 564, 600, 612
BlsI GCNGC 3 cut(s) 565, 601, 613
BmcAI AGTACT 1 cut(s) 136
Bme1390I CCNGG 3 cut(s) 631, 881, 1071
Bme18I GGWCC 4 cut(s) 17, 514, 902, 1456
BmeT110I CYCGRG 3 cut(s) 53, 156, 1099
BmgT120I GGNCC 4 cut(s) 17, 514, 902, 1456
BmiI GGNNCC 3 cut(s) 18, 991, 1209
BmrFI CCNGG 3 cut(s) 631, 881, 1071
BmsI GCATC 6 cut(s) 730, 898, 1063, 1306, 1430, 1531
Bpu14I TTCGAA 1 cut(s) 80
BpuEI CTTGAG 1 cut(s) 590
BpuMI CCSGG 1 cut(s) 881
BsaAI YACGTR 1 cut(s) 302
BsaJI CCNNGG 1 cut(s) 879
BsaXI ACNNNNNCTCC 2 cut(s) 763, 793
Bsc4I CCNNNNNNNGG 1 cut(s) 511
Bse1I ACTGG 4 cut(s) 208, 1089, 1231, 1478
BseBI CCWGG 2 cut(s) 631, 1071
BseDI CCNNGG 1 cut(s) 879
BseGI GGATG 2 cut(s) 964, 1297
BseLI CCNNNNNNNGG 1 cut(s) 511
BseMII CTCAG 1 cut(s) 195
BseNI ACTGG 4 cut(s) 208, 1089, 1231, 1478
BseRI GAGGAG 4 cut(s) 174, 659, 938, 1415
BseXI GCAGC 3 cut(s) 550, 586, 623
BsgI GTGCAG 2 cut(s) 465, 883
Bsh1236I CGCG 1 cut(s) 1007
BshFI GGCC 2 cut(s) 629, 1384
BsiHKAI GWGCWC 2 cut(s) 52, 256
BsiHKCI CYCGRG 3 cut(s) 53, 156, 1099
BsiSI CCGG 1 cut(s) 881
BslFI GGGAC 2 cut(s) 30, 1127
BslI CCNNNNNNNGG 1 cut(s) 511
BsmAI GTCTC 1 cut(s) 812
BsmFI GGGAC 2 cut(s) 30, 1127
BsmI GAATGC 2 cut(s) 565, 1019
BsnI GGCC 2 cut(s) 629, 1384
BsoBI CYCGRG 3 cut(s) 53, 156, 1099
Bsp119I TTCGAA 1 cut(s) 80
Bsp1286I GDGCHC 2 cut(s) 52, 256
Bsp1407I TGTACA 1 cut(s) 471
Bsp143I GATC 8 cut(s) 268, 331, 871, 1002, 1060, 1207, 1451, 1507
BspANI GGCC 2 cut(s) 629, 1384
BspCNI CTCAG 1 cut(s) 194
BspFNI CGCG 1 cut(s) 1007
BspLI GGNNCC 3 cut(s) 18, 991, 1209
BspPI GGATC 4 cut(s) 339, 1202, 1215, 1502
BspQI GCTCTTC 1 cut(s) 483
BspT104I TTCGAA 1 cut(s) 80
BsrGI TGTACA 1 cut(s) 471
BsrI ACTGG 4 cut(s) 208, 1089, 1231, 1478
BssECI CCNNGG 1 cut(s) 879
BssMI GATC 8 cut(s) 268, 331, 871, 1002, 1060, 1207, 1451, 1507
Bst2UI CCWGG 2 cut(s) 631, 1071
Bst4CI ACNGT 4 cut(s) 34, 235, 258, 779
Bst6I CTCTTC 5 cut(s) 354, 483, 547, 600, 752
BstAUI TGTACA 1 cut(s) 471
BstBAI YACGTR 1 cut(s) 302
BstBI TTCGAA 1 cut(s) 80
BstC8I GCNNGC 3 cut(s) 663, 1017, 1125
BstDEI CTNAG 6 cut(s) 181, 272, 814, 950, 1436, 1518
BstF5I GGATG 2 cut(s) 964, 1297
BstFNI CGCG 1 cut(s) 1007
BstKTI GATC 8 cut(s) 271, 334, 874, 1005, 1063, 1210, 1454, 1510
BstMAI GTCTC 1 cut(s) 812
BstMBI GATC 8 cut(s) 268, 331, 871, 1002, 1060, 1207, 1451, 1507
BstMWI GCNNNNNNNGC 3 cut(s) 608, 671, 1415
BstNI CCWGG 2 cut(s) 631, 1071
BstNSI RCATGY 2 cut(s) 472, 503
BstSCI CCNGG 3 cut(s) 629, 879, 1069
BstSFI CTRYAG 1 cut(s) 778
BstUI CGCG 1 cut(s) 1007
BstV1I GCAGC 3 cut(s) 550, 586, 623
BstX2I RGATCY 4 cut(s) 268, 1060, 1207, 1507
BstYI RGATCY 4 cut(s) 268, 1060, 1207, 1507
BsuI GTATCC 1 cut(s) 238
BsuRI GGCC 2 cut(s) 629, 1384
BtsCI GGATG 2 cut(s) 964, 1297
BtsI GCAGTG 1 cut(s) 314
BtsIMutI CAGTG 1 cut(s) 314
Cac8I GCNNGC 3 cut(s) 663, 1017, 1125
Cfr13I GGNCC 4 cut(s) 17, 514, 902, 1456
CsiI ACCWGGT 1 cut(s) 1069
Csp6I GTAC 7 cut(s) 26, 135, 472, 497, 766, 1168, 1191
CspCI CAANNNNNGTGG 2 cut(s) 1541, 1576
CviAII CATG 5 cut(s) 71, 469, 500, 753, 1204
CviQI GTAC 7 cut(s) 26, 135, 472, 497, 766, 1168, 1191
DdeI CTNAG 6 cut(s) 181, 272, 814, 950, 1436, 1518
DpnI GATC 8 cut(s) 270, 333, 873, 1004, 1062, 1209, 1453, 1509
DpnII GATC 8 cut(s) 268, 331, 871, 1002, 1060, 1207, 1451, 1507
EaeI YGGCCR 1 cut(s) 1382
Eam1104I CTCTTC 5 cut(s) 354, 483, 547, 600, 752
EarI CTCTTC 5 cut(s) 354, 483, 547, 600, 752
Eco47I GGWCC 4 cut(s) 17, 514, 902, 1456
Eco57I CTGAAG 6 cut(s) 32, 309, 353, 624, 1488, 1556
Eco88I CYCGRG 3 cut(s) 53, 156, 1099
EcoRI GAATTC 1 cut(s) 533
EcoRII CCWGG 2 cut(s) 629, 1069
FaeI CATG 5 cut(s) 74, 472, 503, 756, 1207
FalI AAGNNNNNCTT 6 cut(s) 199, 231, 351, 383, 645, 677
FaqI GGGAC 2 cut(s) 30, 1127
FatI CATG 5 cut(s) 70, 468, 499, 752, 1203
FbaI TGATCA 1 cut(s) 871
FblI GTMKAC 2 cut(s) 152, 716
Fnu4HI GCNGC 3 cut(s) 564, 600, 612
FokI GGATG 2 cut(s) 951, 1284
Fsp4HI GCNGC 3 cut(s) 564, 600, 612
FspBI CTAG 6 cut(s) 108, 132, 224, 324, 1388, 1512
GluI GCNGC 3 cut(s) 564, 600, 612
HaeIII GGCC 2 cut(s) 629, 1384
HapII CCGG 1 cut(s) 881
Hin1II CATG 5 cut(s) 74, 472, 503, 756, 1207
HincII GTYRAC 1 cut(s) 717
HindII GTYRAC 1 cut(s) 717
HindIII AAGCTT 1 cut(s) 1433
HinfI GANTC 5 cut(s) 128, 343, 1175, 1367, 1491
HpaII CCGG 1 cut(s) 881
HphI GGTGA 1 cut(s) 845
Hpy166II GTNNAC 4 cut(s) 153, 717, 856, 1193
Hpy188III TCNNGA 7 cut(s) 53, 335, 569, 869, 998, 1058, 1211
Hpy8I GTNNAC 4 cut(s) 153, 717, 856, 1193
HpyAV CCTTC 8 cut(s) 7, 188, 236, 377, 619, 778, 830, 913
HpyCH4III ACNGT 4 cut(s) 34, 235, 258, 779
HpyCH4IV ACGT 2 cut(s) 301, 1116
HpyF10VI GCNNNNNNNGC 3 cut(s) 608, 671, 1415
HpyF3I CTNAG 6 cut(s) 181, 272, 814, 950, 1436, 1518
HpySE526I ACGT 2 cut(s) 301, 1116
Hsp92II CATG 5 cut(s) 74, 472, 503, 756, 1207
Ksp22I TGATCA 1 cut(s) 871
Kzo9I GATC 8 cut(s) 268, 331, 871, 1002, 1060, 1207, 1451, 1507
LguI GCTCTTC 1 cut(s) 483
LmnI GCTCC 3 cut(s) 55, 251, 989
Lsp1109I GCAGC 3 cut(s) 550, 586, 623
LweI GCATC 6 cut(s) 730, 898, 1063, 1306, 1430, 1531
MabI ACCWGGT 1 cut(s) 1069
MaeI CTAG 6 cut(s) 108, 132, 224, 324, 1388, 1512
MaeII ACGT 2 cut(s) 301, 1116
MaeIII GTNAC 2 cut(s) 755, 1372
MalI GATC 8 cut(s) 270, 333, 873, 1004, 1062, 1209, 1453, 1509
MboI GATC 8 cut(s) 268, 331, 871, 1002, 1060, 1207, 1451, 1507
MflI RGATCY 4 cut(s) 268, 1060, 1207, 1507
MhlI GDGCHC 2 cut(s) 52, 256
MlsI TGGCCA 1 cut(s) 1384
MluNI TGGCCA 1 cut(s) 1384
MlyI GAGTC 3 cut(s) 137, 1376, 1485
MmeI TCCRAC 1 cut(s) 541
Mox20I TGGCCA 1 cut(s) 1384
MroXI GAANNNNTTC 1 cut(s) 211
MscI TGGCCA 1 cut(s) 1384
MseI TTAA 4 cut(s) 237, 698, 891, 1421
MslI CAYNNNNRTG 2 cut(s) 1423, 1539
Msp20I TGGCCA 1 cut(s) 1384
MspI CCGG 1 cut(s) 881
MspR9I CCNGG 3 cut(s) 631, 881, 1071
Mva1269I GAATGC 2 cut(s) 565, 1019
MvaI CCWGG 2 cut(s) 631, 1071
MvnI CGCG 1 cut(s) 1007
MwoI GCNNNNNNNGC 3 cut(s) 608, 671, 1415
NciI CCSGG 1 cut(s) 881
NdeII GATC 8 cut(s) 268, 331, 871, 1002, 1060, 1207, 1451, 1507
NlaIII CATG 5 cut(s) 74, 472, 503, 756, 1207
NlaIV GGNNCC 3 cut(s) 18, 991, 1209
NmuCI GTSAC 2 cut(s) 755, 1372
NspI RCATGY 2 cut(s) 472, 503
NspV TTCGAA 1 cut(s) 80
PaeR7I CTCGAG 2 cut(s) 156, 1099
PciSI GCTCTTC 1 cut(s) 483
PctI GAATGC 2 cut(s) 565, 1019
PdmI GAANNNNTTC 1 cut(s) 211
PfeI GAWTC 2 cut(s) 343, 1175
PkrI GCNGC 3 cut(s) 565, 601, 613
PleI GAGTC 3 cut(s) 136, 1375, 1485
PpsI GAGTC 3 cut(s) 136, 1375, 1485
Ppu21I YACGTR 1 cut(s) 302
Psp6I CCWGG 2 cut(s) 629, 1069
PspGI CCWGG 2 cut(s) 629, 1069
PspN4I GGNNCC 3 cut(s) 18, 991, 1209
PspPI GGNCC 4 cut(s) 17, 514, 902, 1456
PspXI VCTCGAGB 2 cut(s) 156, 1099
PsuI RGATCY 4 cut(s) 268, 1060, 1207, 1507
RsaI GTAC 7 cut(s) 27, 136, 473, 498, 767, 1169, 1192
RsaNI GTAC 7 cut(s) 26, 135, 472, 497, 766, 1168, 1191
RseI CAYNNNNRTG 2 cut(s) 1423, 1539
SalI GTCGAC 1 cut(s) 715
SapI GCTCTTC 1 cut(s) 483
SaqAI TTAA 4 cut(s) 237, 698, 891, 1421
SatI GCNGC 3 cut(s) 564, 600, 612
Sau3AI GATC 8 cut(s) 268, 331, 871, 1002, 1060, 1207, 1451, 1507
Sau96I GGNCC 4 cut(s) 17, 514, 902, 1456
ScaI AGTACT 1 cut(s) 136
SchI GAGTC 3 cut(s) 137, 1376, 1485
ScrFI CCNGG 3 cut(s) 631, 881, 1071
SduI GDGCHC 2 cut(s) 52, 256
SexAI ACCWGGT 1 cut(s) 1069
SfaNI GCATC 6 cut(s) 730, 898, 1063, 1306, 1430, 1531
SfcI CTRYAG 1 cut(s) 778
Sfr274I CTCGAG 2 cut(s) 156, 1099
SfuI TTCGAA 1 cut(s) 80
SinI GGWCC 4 cut(s) 17, 514, 902, 1456
SlaI CTCGAG 2 cut(s) 156, 1099
SmiMI CAYNNNNRTG 2 cut(s) 1423, 1539
SmlI CTYRAG 3 cut(s) 156, 569, 1099
SmoI CTYRAG 3 cut(s) 156, 569, 1099
SspMI CTAG 6 cut(s) 108, 132, 224, 324, 1388, 1512
StyD4I CCNGG 3 cut(s) 629, 879, 1069
TaaI ACNGT 4 cut(s) 34, 235, 258, 779
TaiI ACGT 2 cut(s) 304, 1119
TaqI TCGA 5 cut(s) 80, 157, 716, 1057, 1100
TatI WGTACW 3 cut(s) 134, 471, 1167
TfiI GAWTC 2 cut(s) 343, 1175
Tru1I TTAA 4 cut(s) 237, 698, 891, 1421
Tru9I TTAA 4 cut(s) 237, 698, 891, 1421
TscAI CASTG 1 cut(s) 314
TseFI GTSAC 2 cut(s) 755, 1372
TseI GCWGC 3 cut(s) 563, 599, 611
Tsp45I GTSAC 2 cut(s) 755, 1372
TspDTI ATGAA 5 cut(s) 59, 203, 207, 255, 399
TspGWI ACGGA 1 cut(s) 1178
TspRI CASTG 1 cut(s) 314
VpaK11BI GGWCC 4 cut(s) 17, 514, 902, 1456
XapI RAATTY 3 cut(s) 533, 731, 945
XceI RCATGY 2 cut(s) 472, 503
XhoI CTCGAG 2 cut(s) 156, 1099
XmiI GTMKAC 2 cut(s) 152, 716
XmnI GAANNNNTTC 1 cut(s) 211
XspI CTAG 6 cut(s) 108, 132, 224, 324, 1388, 1512
ZrmI AGTACT 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.