pycom02g06690

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002207
Physical Location & Seq
Forward (+)
4629778 .. 4630433
656 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g06690.1

Sequence Viewer

Length: 555 bp
ATGGATCCAGAGTATCAAAGAACTGGCACTGTCCGGCCCAAATCAGATTTGTATGCATTTGGTGTCATAATCCTTCAAGTACTGACAGGTCGCCATCCAAAGCGGCTTATATTTGTTACGGAGAATGCAATTGCAAGTGGGTCTTTTGCTGAAATCTTGGACAAGTCGGTTGCAGATTGGCCACTTGCAGAAGCAGAGGAGTTGGCTCGAGTTGCACTAAGGTGTTCCCAACTTAGATGCAGGGATAGACCAGATCTTGAAACTGAAGTTTTGCCGGTTCTCAAACGACTCGCTAATCTTGCAGATTCTAGCTTGAAGTTTGATAGAAACCCTATCAATGCACCAAACCACTTCTTTTGTCCAATACTTCAGGAAATCATGGAGGATCCACACGTAGCTGCAGACGGTTTTACATATGAGTATCGAGCGATCAAAGCATGGATTAAGAAACACAAAGTGTCCCCATTGACAAGGCTTAGACTAAACAATTTTGTGTTGACTCCAAACCATACATTGCGTTCTGCCATACAGGAATGGAGGTCAGGTTTGACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr U-box Usp

Protein Analysis

185

Amino Acids

20.99

Weight (kDa)

8.82

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 114 - 180 4.3e-17 U-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 103
AclWI GGATC 3 cut(s) 12, 380, 393
AcoI YGGCCR 1 cut(s) 179
AcuI CTGAAG 2 cut(s) 285, 353
AdeI CACNNNGTG 1 cut(s) 457
AfaI GTAC 1 cut(s) 81
AflIII ACRYGT 1 cut(s) 391
AgsI TTSAA 3 cut(s) 77, 260, 316
AleI CACNNNNGTG 1 cut(s) 220
AluBI AGCT 2 cut(s) 312, 398
AluI AGCT 2 cut(s) 312, 398
AlwI GGATC 3 cut(s) 12, 380, 393
Ama87I CYCGRG 1 cut(s) 207
AoxI GGCC 2 cut(s) 35, 179
ApeKI GCWGC 1 cut(s) 398
AspS9I GGNCC 1 cut(s) 36
AvaI CYCGRG 1 cut(s) 207
BalI TGGCCA 1 cut(s) 181
BamHI GGATCC 2 cut(s) 4, 385
BbvI GCAGC 1 cut(s) 385
BccI CCATC 1 cut(s) 102
BfaI CTAG 1 cut(s) 309
BfmI CTRYAG 1 cut(s) 399
BglII AGATCT 1 cut(s) 253
BisI GCNGC 2 cut(s) 104, 399
BlsI GCNGC 2 cut(s) 105, 400
BmcAI AGTACT 1 cut(s) 81
BmeT110I CYCGRG 1 cut(s) 207
BmgT120I GGNCC 1 cut(s) 36
BmiI GGNNCC 2 cut(s) 6, 387
BmsI GCATC 1 cut(s) 227
BsaAI YACGTR 1 cut(s) 394
BsaXI ACNNNNNCTCC 1 cut(s) 529
Bse118I RCCGGY 1 cut(s) 274
Bse1I ACTGG 1 cut(s) 28
Bse3DI GCAATG 1 cut(s) 512
BseGI GGATG 1 cut(s) 94
BseMI GCAATG 1 cut(s) 512
BseNI ACTGG 1 cut(s) 28
BseRI GAGGAG 1 cut(s) 212
BseXI GCAGC 1 cut(s) 385
BshFI GGCC 2 cut(s) 37, 181
BsiHKCI CYCGRG 1 cut(s) 207
BsiSI CCGG 2 cut(s) 34, 275
BslFI GGGAC 1 cut(s) 445
BsmFI GGGAC 1 cut(s) 445
BsmI GAATGC 1 cut(s) 130
BsnI GGCC 2 cut(s) 37, 181
BsoBI CYCGRG 1 cut(s) 207
Bsp143I GATC 4 cut(s) 4, 253, 385, 429
BspACI CCGC 1 cut(s) 103
BspANI GGCC 2 cut(s) 37, 181
BspLI GGNNCC 2 cut(s) 6, 387
BspMAI CTGCAG 1 cut(s) 403
BspPI GGATC 3 cut(s) 12, 380, 393
BsrDI GCAATG 1 cut(s) 512
BsrFI RCCGGY 1 cut(s) 274
BsrI ACTGG 1 cut(s) 28
BssAI RCCGGY 1 cut(s) 274
BssMI GATC 4 cut(s) 4, 253, 385, 429
Bst4CI ACNGT 2 cut(s) 31, 407
BstBAI YACGTR 1 cut(s) 394
BstDEI CTNAG 3 cut(s) 218, 233, 476
BstF5I GGATG 1 cut(s) 94
BstKTI GATC 4 cut(s) 7, 256, 388, 432
BstMBI GATC 4 cut(s) 4, 253, 385, 429
BstMWI GCNNNNNNNGC 3 cut(s) 212, 299, 434
BstSFI CTRYAG 1 cut(s) 399
BstV1I GCAGC 1 cut(s) 385
BstX2I RGATCY 3 cut(s) 4, 253, 385
BstYI RGATCY 3 cut(s) 4, 253, 385
BsuRI GGCC 2 cut(s) 37, 181
BtsCI GGATG 1 cut(s) 94
BtsIMutI CAGTG 1 cut(s) 27
Cfr10I RCCGGY 1 cut(s) 274
Cfr13I GGNCC 1 cut(s) 36
Csp6I GTAC 1 cut(s) 80
CspCI CAANNNNNGTGG 2 cut(s) 338, 373
CviAII CATG 2 cut(s) 379, 438
CviJI RGCY 7 cut(s) 37, 106, 181, 206, 312, 398, 475
CviKI_1 RGCY 7 cut(s) 37, 106, 181, 206, 312, 398, 475
CviQI GTAC 1 cut(s) 80
DdeI CTNAG 3 cut(s) 218, 233, 476
DpnI GATC 4 cut(s) 6, 255, 387, 431
DpnII GATC 4 cut(s) 4, 253, 385, 429
DraIII CACNNNGTG 1 cut(s) 457
EaeI YGGCCR 1 cut(s) 179
Eco57I CTGAAG 2 cut(s) 285, 353
Eco88I CYCGRG 1 cut(s) 207
EcoT22I ATGCAT 1 cut(s) 58
FaeI CATG 2 cut(s) 382, 441
FaiI YATR 9 cut(s) 54, 68, 110, 380, 415, 417, 439, 510, 527
FalI AAGNNNNNCTT 2 cut(s) 127, 159
FaqI GGGAC 1 cut(s) 445
FatI CATG 2 cut(s) 378, 437
FauNDI CATATG 1 cut(s) 415
Fnu4HI GCNGC 2 cut(s) 104, 399
FokI GGATG 1 cut(s) 81
Fsp4HI GCNGC 2 cut(s) 104, 399
FspBI CTAG 1 cut(s) 309
GluI GCNGC 2 cut(s) 104, 399
HaeIII GGCC 2 cut(s) 37, 181
HapII CCGG 2 cut(s) 34, 275
Hin1II CATG 2 cut(s) 382, 441
HincII GTYRAC 1 cut(s) 498
HindII GTYRAC 1 cut(s) 498
HinfI GANTC 3 cut(s) 288, 305, 499
HpaII CCGG 2 cut(s) 34, 275
Hpy166II GTNNAC 1 cut(s) 498
Hpy188I TCNGA 1 cut(s) 46
Hpy188III TCNNGA 3 cut(s) 8, 257, 371
Hpy8I GTNNAC 1 cut(s) 498
HpyAV CCTTC 1 cut(s) 83
HpyCH4III ACNGT 2 cut(s) 31, 407
HpyCH4IV ACGT 1 cut(s) 393
HpyF10VI GCNNNNNNNGC 3 cut(s) 212, 299, 434
HpyF3I CTNAG 3 cut(s) 218, 233, 476
HpySE526I ACGT 1 cut(s) 393
Hsp92II CATG 2 cut(s) 382, 441
Kzo9I GATC 4 cut(s) 4, 253, 385, 429
Lsp1109I GCAGC 1 cut(s) 385
LweI GCATC 1 cut(s) 227
MaeI CTAG 1 cut(s) 309
MaeII ACGT 1 cut(s) 393
MaeIII GTNAC 1 cut(s) 115
MalI GATC 4 cut(s) 6, 255, 387, 431
MboI GATC 4 cut(s) 4, 253, 385, 429
MfeI CAATTG 1 cut(s) 129
MflI RGATCY 3 cut(s) 4, 253, 385
MlsI TGGCCA 1 cut(s) 181
MluCI AATT 2 cut(s) 129, 487
MluNI TGGCCA 1 cut(s) 181
MlyI GAGTC 2 cut(s) 282, 493
MnlI CCTC 3 cut(s) 190, 376, 531
Mox20I TGGCCA 1 cut(s) 181
Mph1103I ATGCAT 1 cut(s) 58
MscI TGGCCA 1 cut(s) 181
MseI TTAA 2 cut(s) 444, 553
MslI CAYNNNNRTG 1 cut(s) 220
Msp20I TGGCCA 1 cut(s) 181
MspI CCGG 2 cut(s) 34, 275
MunI CAATTG 1 cut(s) 129
Mva1269I GAATGC 1 cut(s) 130
MwoI GCNNNNNNNGC 3 cut(s) 212, 299, 434
NdeI CATATG 1 cut(s) 415
NdeII GATC 4 cut(s) 4, 253, 385, 429
NlaIII CATG 2 cut(s) 382, 441
NlaIV GGNNCC 2 cut(s) 6, 387
NsiI ATGCAT 1 cut(s) 58
OliI CACNNNNGTG 1 cut(s) 220
PaeR7I CTCGAG 1 cut(s) 207
PctI GAATGC 1 cut(s) 130
PfeI GAWTC 1 cut(s) 305
PkrI GCNGC 2 cut(s) 105, 400
PleI GAGTC 2 cut(s) 282, 493
PpsI GAGTC 2 cut(s) 282, 493
Ppu21I YACGTR 1 cut(s) 394
PspN4I GGNNCC 2 cut(s) 6, 387
PspPI GGNCC 1 cut(s) 36
PspXI VCTCGAGB 1 cut(s) 207
PstI CTGCAG 1 cut(s) 403
PsuI RGATCY 3 cut(s) 4, 253, 385
RsaI GTAC 1 cut(s) 81
RsaNI GTAC 1 cut(s) 80
RseI CAYNNNNRTG 1 cut(s) 220
SaqAI TTAA 2 cut(s) 444, 553
SatI GCNGC 2 cut(s) 104, 399
Sau3AI GATC 4 cut(s) 4, 253, 385, 429
Sau96I GGNCC 1 cut(s) 36
ScaI AGTACT 1 cut(s) 81
SchI GAGTC 2 cut(s) 282, 493
SetI ASST 7 cut(s) 91, 224, 314, 396, 400, 542, 547
SfaNI GCATC 1 cut(s) 227
SfcI CTRYAG 1 cut(s) 399
Sfr274I CTCGAG 1 cut(s) 207
SlaI CTCGAG 1 cut(s) 207
SmiMI CAYNNNNRTG 1 cut(s) 220
SmlI CTYRAG 1 cut(s) 207
SmoI CTYRAG 1 cut(s) 207
Sse9I AATT 2 cut(s) 129, 487
SsiI CCGC 1 cut(s) 103
SspMI CTAG 1 cut(s) 309
TaaI ACNGT 2 cut(s) 31, 407
TaiI ACGT 1 cut(s) 396
TaqI TCGA 2 cut(s) 208, 424
TasI AATT 2 cut(s) 129, 487
TatI WGTACW 1 cut(s) 79
TauI GCSGC 1 cut(s) 106
TfiI GAWTC 1 cut(s) 305
Tru1I TTAA 2 cut(s) 444, 553
Tru9I TTAA 2 cut(s) 444, 553
TscAI CASTG 1 cut(s) 34
TseI GCWGC 1 cut(s) 398
TspGWI ACGGA 1 cut(s) 134
TspRI CASTG 1 cut(s) 34
XhoI CTCGAG 1 cut(s) 207
XspI CTAG 1 cut(s) 309
ZrmI AGTACT 1 cut(s) 81
Zsp2I ATGCAT 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.