RchiOBHm_Chr2g0094001

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002207
Physical Location & Seq
Forward (+)
7076118 .. 7080286
4169 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46903

Sequence Viewer

Length: 1407 bp
ATGTACAAACGAGCTTGTGAAGAGCTGGTACATGCCCAAAGCAAGGTCCAACTACTTTCTTCCGAATTCCTTGAAGAAGCAAGAAGAGTGAATGCTGCTCTTGAGAGTGAGGAAACTTTGAGAAAAATTGCTGCTGAAGAGGCAGCAAAGCATTTGAAGGCCAGGAAAGAGATTGAGGAGGCAAAAGTTCTGCTTGCTAAAGAGGCTTATGAGAGGCAAGTTGCTGAATTAAATGCCCTGAAAAAGTCGACAGGGAAAAAGGAATTTGTTGATGCACTCTTCTCATGTGACAATAGGTACAGGAGATACTGTAGGAAGGGAATAGAGATAGCAACAAACTTCTTCTTAGAGACTAATGTGATTGGTGAAGGAGGATATGGGAAAGTTTACAAGTGCAGTCTTGATCAAACCCCGGTAGTTGTTAAGGTTCTTGGACCTGATGCAGTTGAGAAGGAGGAGTTTCTGAAAGAGGTTGAAATTCTTAGTCAGCTACATCATCCTAACATAGTTTTGCTTCTTGGAGCCTGTCCAGAGATCGCGTGTGGGCTTGCATTCTTACACAACTCAAAGACAGACCCTATTGTGCATCGAGATCTAAAACCAGGTAATGTCTTACTGGACAGAAACTACTCGAGCAAAATTGGGGACGTTGGGCTGGCTAAACTCATTACAGACATCGTGCCTGACAACATAACGGAGTACAGAGAATCAATTATTGCTGGTACACTCTTTTACATGGATCCAGAGTATCAGAGAACTGGCACAATTAGACCCAAATCGGATTTATACTCATTTGGGGTCATTATCCTCCAAGTATTGACAGCACGGCATCCAAATAGGCTTATATACATTGTGGAAAATGCACTTGCAAATGGTTCTTTTGCCGACTTTTTAGATGAGTCAGTCACAGATTGGCCACTAGAAGAAACAGAGGAGTTGGCTCAACTTGCATTAAGGTGTTCAAAGCTTAGATGCAGGGATAGATCGGACCTTGAAACTGAAGTTCTGCCAGTTCTCAAAAGACTCCTTGATATTGCAGATCCTAGTTCTAAGATTGAGAGAAACCATATTGATGCACCAAGCCACTACTATTGTCCAATACTTCAGGAAATCATGGACGACCCACACATTTCTGCCGATGGCTTTACTTATGAGTACAGAGCAATCAAAGCATGGCTTGACAAACACAATGTATCCCCATTGACAAGGCTTAGACTTCAGCATTCAGAGTTAACTCCTAACCATACATTGCGTTCAGCTATACAAGAATGGAGGTCAAGCGTGACATACTCTACTGCCTTAGTGGATAGACGAGTTGATTGCACCAGTTCTTGCAAGCTCTCCAAAGCAGAAACTACATATCAGGAGTATAGTTGTCTTATATTTTTAACTTGGATGCTCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr U-box Usp

Protein Analysis

468

Amino Acids

53.31

Weight (kDa)

5.89

Isoelectric Point (pI)

51.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 118 - 176 5.2e-08 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 119 - 176 1.5e-10 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 178 - 328 8.5e-19 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 178 - 332 6.9e-16 Protein tyrosine and serine/threonine kinase
U-box PF04564 359 - 426 1.5e-17 U-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 248
AccII CGCG 1 cut(s) 539
AclWI GGATC 3 cut(s) 734, 747, 1034
AcoI YGGCCR 1 cut(s) 914
AcsI RAATTY 3 cut(s) 65, 263, 477
AcuI CTGAAG 4 cut(s) 156, 1020, 1088, 1202
AfaI GTAC 6 cut(s) 5, 30, 299, 701, 724, 1157
AfiI CCNNNNNNNGG 1 cut(s) 43
AgsI TTSAA 5 cut(s) 74, 157, 476, 963, 995
AjnI CCWGG 2 cut(s) 161, 601
AluBI AGCT 6 cut(s) 14, 25, 490, 967, 1259, 1339
AluI AGCT 6 cut(s) 14, 25, 490, 967, 1259, 1339
Alw26I GTCTC 1 cut(s) 344
AlwI GGATC 3 cut(s) 734, 747, 1034
Ama87I CYCGRG 1 cut(s) 631
AoxI GGCC 2 cut(s) 159, 914
ApeKI GCWGC 3 cut(s) 95, 131, 143
ApoI RAATTY 3 cut(s) 65, 263, 477
AspS9I GGNCC 3 cut(s) 46, 434, 988
AsuC2I CCSGG 1 cut(s) 413
AsuHPI GGTGA 1 cut(s) 377
AvaI CYCGRG 1 cut(s) 631
AvaII GGWCC 3 cut(s) 46, 434, 988
BalI TGGCCA 1 cut(s) 916
BamHI GGATCC 1 cut(s) 739
BarI GAAGNNNNNNTAC 2 cut(s) 12, 44
BbvI GCAGC 3 cut(s) 82, 118, 155
BccI CCATC 1 cut(s) 1133
BceAI ACGGC 1 cut(s) 842
BcgI CGANNNNNNTGC 2 cut(s) 1302, 1336
BciT130I CCWGG 2 cut(s) 163, 603
BciVI GTATCC 1 cut(s) 1204
BclI TGATCA 1 cut(s) 403
BcnI CCSGG 1 cut(s) 413
BcoDI GTCTC 1 cut(s) 344
BfaI CTAG 2 cut(s) 920, 1044
BfmI CTRYAG 1 cut(s) 310
BfuI GTATCC 1 cut(s) 1204
BglII AGATCT 1 cut(s) 592
BisI GCNGC 3 cut(s) 96, 132, 144
BlsI GCNGC 3 cut(s) 97, 133, 145
Bme1390I CCNGG 3 cut(s) 163, 413, 603
Bme18I GGWCC 3 cut(s) 46, 434, 988
BmeT110I CYCGRG 1 cut(s) 631
BmgT120I GGNCC 3 cut(s) 46, 434, 988
BmiI GGNNCC 2 cut(s) 523, 741
BmrFI CCNGG 3 cut(s) 163, 413, 603
BmsI GCATC 7 cut(s) 262, 430, 595, 838, 962, 1063, 1386
BpuEI CTTGAG 1 cut(s) 122
BpuMI CCSGG 1 cut(s) 413
BsaJI CCNNGG 1 cut(s) 411
BsaXI ACNNNNNCTCC 2 cut(s) 295, 325
Bsc4I CCNNNNNNNGG 1 cut(s) 43
Bse1I ACTGG 4 cut(s) 621, 763, 1010, 1326
Bse3DI GCAATG 1 cut(s) 1247
BseBI CCWGG 2 cut(s) 163, 603
BseDI CCNNGG 1 cut(s) 411
BseGI GGATG 3 cut(s) 496, 829, 1401
BseLI CCNNNNNNNGG 1 cut(s) 43
BseMI GCAATG 1 cut(s) 1247
BseNI ACTGG 4 cut(s) 621, 763, 1010, 1326
BseRI GAGGAG 3 cut(s) 191, 470, 947
BseXI GCAGC 3 cut(s) 82, 118, 155
BsgI GTGCAG 1 cut(s) 415
Bsh1236I CGCG 1 cut(s) 539
BshFI GGCC 2 cut(s) 161, 916
BsiHKCI CYCGRG 1 cut(s) 631
BsiSI CCGG 1 cut(s) 413
BslFI GGGAC 1 cut(s) 659
BslI CCNNNNNNNGG 1 cut(s) 43
BsmAI GTCTC 1 cut(s) 344
BsmFI GGGAC 1 cut(s) 659
BsmI GAATGC 3 cut(s) 97, 551, 1222
BsnI GGCC 2 cut(s) 161, 916
BsoBI CYCGRG 1 cut(s) 631
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 6 cut(s) 403, 534, 592, 739, 983, 1039
BspANI GGCC 2 cut(s) 161, 916
BspFNI CGCG 1 cut(s) 539
BspLI GGNNCC 2 cut(s) 523, 741
BspPI GGATC 3 cut(s) 734, 747, 1034
BspQI GCTCTTC 1 cut(s) 15
BsrDI GCAATG 1 cut(s) 1247
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 4 cut(s) 621, 763, 1010, 1326
BssECI CCNNGG 1 cut(s) 411
BssMI GATC 6 cut(s) 403, 534, 592, 739, 983, 1039
Bst2UI CCWGG 2 cut(s) 163, 603
Bst4CI ACNGT 1 cut(s) 311
Bst6I CTCTTC 4 cut(s) 15, 79, 132, 284
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 4 cut(s) 195, 549, 657, 1337
BstDEI CTNAG 7 cut(s) 346, 482, 968, 1050, 1211, 1300, 1400
BstF5I GGATG 3 cut(s) 496, 829, 1401
BstFNI CGCG 1 cut(s) 539
BstKTI GATC 6 cut(s) 406, 537, 595, 742, 986, 1042
BstMAI GTCTC 1 cut(s) 344
BstMBI GATC 6 cut(s) 403, 534, 592, 739, 983, 1039
BstMWI GCNNNNNNNGC 4 cut(s) 140, 203, 947, 1169
BstNI CCWGG 2 cut(s) 163, 603
BstNSI RCATGY 1 cut(s) 35
BstSCI CCNGG 3 cut(s) 161, 411, 601
BstSFI CTRYAG 1 cut(s) 310
BstUI CGCG 1 cut(s) 539
BstV1I GCAGC 3 cut(s) 82, 118, 155
BstX2I RGATCY 3 cut(s) 592, 739, 1039
BstYI RGATCY 3 cut(s) 592, 739, 1039
BsuI GTATCC 1 cut(s) 1204
BsuRI GGCC 2 cut(s) 161, 916
BtsCI GGATG 3 cut(s) 496, 829, 1401
Cac8I GCNNGC 4 cut(s) 195, 549, 657, 1337
Cfr13I GGNCC 3 cut(s) 46, 434, 988
CsiI ACCWGGT 1 cut(s) 601
Csp6I GTAC 6 cut(s) 4, 29, 298, 700, 723, 1156
CspCI CAANNNNNGTGG 2 cut(s) 1073, 1108
CviAII CATG 5 cut(s) 32, 285, 736, 1114, 1173
CviQI GTAC 6 cut(s) 4, 29, 298, 700, 723, 1156
DdeI CTNAG 7 cut(s) 346, 482, 968, 1050, 1211, 1300, 1400
DpnI GATC 6 cut(s) 405, 536, 594, 741, 985, 1041
DpnII GATC 6 cut(s) 403, 534, 592, 739, 983, 1039
EaeI YGGCCR 1 cut(s) 914
Eam1104I CTCTTC 4 cut(s) 15, 79, 132, 284
EarI CTCTTC 4 cut(s) 15, 79, 132, 284
Eco47I GGWCC 3 cut(s) 46, 434, 988
Eco57I CTGAAG 4 cut(s) 156, 1020, 1088, 1202
Eco88I CYCGRG 1 cut(s) 631
EcoRI GAATTC 1 cut(s) 65
EcoRII CCWGG 2 cut(s) 161, 601
FaeI CATG 5 cut(s) 35, 288, 739, 1117, 1176
FalI AAGNNNNNCTT 4 cut(s) 177, 209, 1161, 1193
FaqI GGGAC 1 cut(s) 659
FatI CATG 5 cut(s) 31, 284, 735, 1113, 1172
FbaI TGATCA 1 cut(s) 403
FblI GTMKAC 1 cut(s) 248
Fnu4HI GCNGC 3 cut(s) 96, 132, 144
FokI GGATG 2 cut(s) 483, 816
Fsp4HI GCNGC 3 cut(s) 96, 132, 144
FspBI CTAG 2 cut(s) 920, 1044
GluI GCNGC 3 cut(s) 96, 132, 144
HaeIII GGCC 2 cut(s) 161, 916
HapII CCGG 1 cut(s) 413
Hin1II CATG 5 cut(s) 35, 288, 739, 1117, 1176
HincII GTYRAC 2 cut(s) 249, 1233
HindII GTYRAC 2 cut(s) 249, 1233
HindIII AAGCTT 1 cut(s) 965
HinfI GANTC 3 cut(s) 707, 899, 1023
HpaI GTTAAC 1 cut(s) 1233
HpaII CCGG 1 cut(s) 413
HphI GGTGA 1 cut(s) 377
Hpy166II GTNNAC 4 cut(s) 249, 388, 725, 1233
Hpy188I TCNGA 7 cut(s) 64, 465, 753, 781, 988, 1228, 1403
Hpy188III TCNNGA 7 cut(s) 101, 401, 530, 590, 743, 1106, 1364
Hpy8I GTNNAC 4 cut(s) 249, 388, 725, 1233
HpyAV CCTTC 4 cut(s) 151, 310, 362, 445
HpyCH4III ACNGT 1 cut(s) 311
HpyCH4IV ACGT 1 cut(s) 648
HpyF10VI GCNNNNNNNGC 4 cut(s) 140, 203, 947, 1169
HpyF3I CTNAG 7 cut(s) 346, 482, 968, 1050, 1211, 1300, 1400
HpySE526I ACGT 1 cut(s) 648
Hsp92II CATG 5 cut(s) 35, 288, 739, 1117, 1176
Ksp22I TGATCA 1 cut(s) 403
KspAI GTTAAC 1 cut(s) 1233
Kzo9I GATC 6 cut(s) 403, 534, 592, 739, 983, 1039
LguI GCTCTTC 1 cut(s) 15
LmnI GCTCC 1 cut(s) 521
Lsp1109I GCAGC 3 cut(s) 82, 118, 155
LweI GCATC 7 cut(s) 262, 430, 595, 838, 962, 1063, 1386
MabI ACCWGGT 1 cut(s) 601
MaeI CTAG 2 cut(s) 920, 1044
MaeII ACGT 1 cut(s) 648
MaeIII GTNAC 3 cut(s) 287, 904, 1282
MalI GATC 6 cut(s) 405, 536, 594, 741, 985, 1041
MboI GATC 6 cut(s) 403, 534, 592, 739, 983, 1039
MboII GAAGA 8 cut(s) 32, 51, 86, 96, 149, 271, 334, 935
MflI RGATCY 3 cut(s) 592, 739, 1039
MlsI TGGCCA 1 cut(s) 916
MluCI AATT 8 cut(s) 65, 126, 227, 263, 477, 639, 711, 765
MluNI TGGCCA 1 cut(s) 916
MlyI GAGTC 2 cut(s) 908, 1017
MmeI TCCRAC 1 cut(s) 73
Mox20I TGGCCA 1 cut(s) 916
MscI TGGCCA 1 cut(s) 916
MseI TTAA 5 cut(s) 230, 423, 953, 1232, 1388
MslI CAYNNNNRTG 2 cut(s) 955, 1071
Msp20I TGGCCA 1 cut(s) 916
MspI CCGG 1 cut(s) 413
MspR9I CCNGG 3 cut(s) 163, 413, 603
Mva1269I GAATGC 3 cut(s) 97, 551, 1222
MvaI CCWGG 2 cut(s) 163, 603
MvnI CGCG 1 cut(s) 539
MwoI GCNNNNNNNGC 4 cut(s) 140, 203, 947, 1169
NciI CCSGG 1 cut(s) 413
NdeII GATC 6 cut(s) 403, 534, 592, 739, 983, 1039
NlaIII CATG 5 cut(s) 35, 288, 739, 1117, 1176
NlaIV GGNNCC 2 cut(s) 523, 741
NmuCI GTSAC 3 cut(s) 287, 904, 1282
NspI RCATGY 1 cut(s) 35
PaeR7I CTCGAG 1 cut(s) 631
PciSI GCTCTTC 1 cut(s) 15
PctI GAATGC 3 cut(s) 97, 551, 1222
PfeI GAWTC 1 cut(s) 707
PkrI GCNGC 3 cut(s) 97, 133, 145
PleI GAGTC 2 cut(s) 907, 1017
PpsI GAGTC 2 cut(s) 907, 1017
Psp6I CCWGG 2 cut(s) 161, 601
PspGI CCWGG 2 cut(s) 161, 601
PspN4I GGNNCC 2 cut(s) 523, 741
PspPI GGNCC 3 cut(s) 46, 434, 988
PspXI VCTCGAGB 1 cut(s) 631
PsuI RGATCY 3 cut(s) 592, 739, 1039
RsaI GTAC 6 cut(s) 5, 30, 299, 701, 724, 1157
RsaNI GTAC 6 cut(s) 4, 29, 298, 700, 723, 1156
RseI CAYNNNNRTG 2 cut(s) 955, 1071
SalI GTCGAC 1 cut(s) 247
SapI GCTCTTC 1 cut(s) 15
SaqAI TTAA 5 cut(s) 230, 423, 953, 1232, 1388
SatI GCNGC 3 cut(s) 96, 132, 144
Sau3AI GATC 6 cut(s) 403, 534, 592, 739, 983, 1039
Sau96I GGNCC 3 cut(s) 46, 434, 988
SchI GAGTC 2 cut(s) 908, 1017
ScrFI CCNGG 3 cut(s) 163, 413, 603
SexAI ACCWGGT 1 cut(s) 601
SfaNI GCATC 7 cut(s) 262, 430, 595, 838, 962, 1063, 1386
SfcI CTRYAG 1 cut(s) 310
Sfr274I CTCGAG 1 cut(s) 631
SinI GGWCC 3 cut(s) 46, 434, 988
SlaI CTCGAG 1 cut(s) 631
SmiMI CAYNNNNRTG 2 cut(s) 955, 1071
SmlI CTYRAG 2 cut(s) 101, 631
SmoI CTYRAG 2 cut(s) 101, 631
Sse9I AATT 8 cut(s) 65, 126, 227, 263, 477, 639, 711, 765
SspMI CTAG 2 cut(s) 920, 1044
StyD4I CCNGG 3 cut(s) 161, 411, 601
TaaI ACNGT 1 cut(s) 311
TaiI ACGT 1 cut(s) 651
TaqI TCGA 3 cut(s) 248, 589, 632
TasI AATT 8 cut(s) 65, 126, 227, 263, 477, 639, 711, 765
TatI WGTACW 3 cut(s) 3, 699, 1155
TfiI GAWTC 1 cut(s) 707
Tru1I TTAA 5 cut(s) 230, 423, 953, 1232, 1388
Tru9I TTAA 5 cut(s) 230, 423, 953, 1232, 1388
TseFI GTSAC 3 cut(s) 287, 904, 1282
TseI GCWGC 3 cut(s) 95, 131, 143
Tsp45I GTSAC 3 cut(s) 287, 904, 1282
TspGWI ACGGA 1 cut(s) 710
VpaK11BI GGWCC 3 cut(s) 46, 434, 988
XapI RAATTY 3 cut(s) 65, 263, 477
XceI RCATGY 1 cut(s) 35
XhoI CTCGAG 1 cut(s) 631
XmiI GTMKAC 1 cut(s) 248
XspI CTAG 2 cut(s) 920, 1044
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.