Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0002207

Physical Location & Seq

Genomic Coordinates Forward (+)

6666943 .. 6670517

Gene Structure 3575 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016442

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2292 bp

ATGACGTCAGTGGCGGTAGCGGTCAACGGCGGCGACGGAAACGGAAGCCGACGCGCCGTGAAGTGGGCGGTGGAGAATCTCATGCCCACGGCCGATCGCTTCGTATTGGTCCACGTCATGCCCAGAATCACTTCAATTCCAACTCCATCAGGAGATCGCATCCCGGTGACGGAGCTAGACGAGGGTGTGGTGGCGACGTATGTGAAGGACATGAAGGACAAGTACGAGGAGGTTTTTACTCCATTCAAAAAGCTATGCAAGTCAAAGAAGGTTGAAACATTGGTATTGGAGGATGAGGTTCCTGCAAATGGGATTCTAAGCTTTATATCTCAGTCTGATATCAATTGTTTAGTTTTGGGCTCTTGCAATTCCAATTACATAACCAGGAAACTGAAGGGACCAGATGTACCGACAGTTGTTCTGAAATGTGCTCCCGAGAGTTGTGATATTCATGTCGTTTCGAAACAAAGAGTTATCACAAAATCAGCTAGTCTCTCGTCTGCTTGTGAGTCTAGTACTGCTAACGGGCTGTCTACTAGAGGAGAAGATGAGGGGTTTTCCAGAGATATCAATGAACGGATAACTGGAAGTCGTTCTTCCTACCTAGTATCCACAGTTAATGAAGGTTATGGAGCACCGTCAATGTCAGACCTTAGTTATCTAAGTTCTGAAGCATTTACGCGTATGGAATTTCCTGCAAACGCTAGTGTGGATCAGGAAAGGAATCATCAAAACTTGGAAGAGAACCATGGGATTATTTCTTTCCAGCGTGGAAGTTCCCCAATTTCTTCAAACATCGAGCAGTCATATGTGCAGATTGAAGTGGAAAAGCTACGTGCAGAATTGCAAAATACTCTTGGCATGTACAAACGAGCTTGTGAAGAGCTGGTACATGCCCAAAGCAAGGTCCAACTACTTTCTTCCGAATGCCTTGAAGAAGCAAGAAGAGTGAATGCTGCTCTTGAGAGTGAGGAAACTTTGAGAAAAATTGCTGCTGAAGAGGCAGCAAAGCATTTGAAGGCCAGGAAAGAGATTGAGGAGGCAAAAGTTCTGCTTGCTAAAGAGGCTTATGAGAGGCAAGTTGCTGAAATAAATGCCCTGAAAGAGTCGACAGGGAAAAAGGAATTTGTTGATGCACTCTTCTCATGTGACAATAGGTACAGGAGATACTGTAGGAAGGAAATAGAGATAGCAACAAACTTCTTCTCAGAGACTAATGTGATTGGTGAAGGAGGATATGGGAAAGTTTACAAGTGCAGTCTTGATCAAACCCCGGTAGCTGTTAAGGTTCTTCGACCTGATGCAGTTGAGAAGAAGGAGGAGTTTCTGAAAGAGGTTGAAATTCTTAGTCAGCTACATCATCCTAACATAGTTTTGCTTCTTGGAGCCTGTCCAGAGATCGGTTGCCTGGTCTATGAATACCTGGAAAATGGGAGCTTGGAAGATTATATCTCCCACCGCCGGAATGGAAAGCCATCTCTCCCTTGGACTGTTAGGTTCAGGATAATTTTTGAAATAGCGTGTGGGCTTGCGTTCTTACACAACTCAAAGACAGACCCTATTGTGCATCGAGATCTAAAACCTGGTAATGTCTTACTGGACAGAAACTACTCGAGCAAAATTGGGGACGTTGGGCTGTCTAAACTCCTTACAGACATCGTGCCTGATAACATAACGGAGTACAGAGAATCAATTATTGCTGGTACAGTGTTTTACGTGGATCCAGAGTATCAGAGAACTGGCACAATTAGACCCAAATCGGATTTATACGCATTTGGGGTCATTATCCTCCAAGTATTGACAGCACGGCATCCAAATAGGCTTATATACATTGTGGAAAATGCAATTGCAAATGGTTCTTTTGCCGACTTTTTAGATGAGTCAGTCACAGATTGGCCACTAGAAGAAACAGAGGAGTTGGCTCAACTTGCATTAAGTTGTTCAAAGCTTAGATGCAGGGATAGACCGGATCTTGAAACTGAAGTTCTGCCAGTTCTCAAAAGACTAGTTGATTTTGCCGATCCTAGTTCTAAGATTGAGAGAAACCATATTGATGCACCAAGCCACTACTATTGTCCAATACTTCAGGAAATTATGGACGACCCACACATTTCTGCCGATGGCTTTACCTATGAGTACAGAGCAATCAAAGCATGGCTTGACAAACACAATGTATCCCCGGTGACAAGGCTTAGACTTCAGCATTCAGAGTTAACTCCTAACCATACATTGCGTTCAGCTATACAAGAATGGAGGTCACGTGTGTCATACCCTACCGCCTATGTGGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr U-box Usp

Protein Analysis

764

Amino Acids

85.66

Weight (kDa)

5.58

Isoelectric Point (pI)

49.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	403 - 657	5.6e-38	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	405 - 659	1.2e-40	Protein tyrosine and serine/threonine kinase
U-box	PF04564	686 - 753	2e-17	U-box domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002207)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19410 AT2G19410 AT2G19410 AT5G57035
fragaria_vesca	FvH4_1g07970 FvH4_1g07970 FvH4_1g07970 FvH4_1g07970 FvH4_1g07970
malus_domestica	MD02G1084100.v1.1
prunus_persica	Prupe.7G205400_v2.0.a1 Prupe.7G205400_v2.0.a1 Prupe.7G205400_v2.0.a1 Prupe.7G205400_v2.0.a1 Prupe.7G205400_v2.0.a1 Prupe.7G205400_v2.0.a1 Prupe.7G205400_v2.0.a1 Prupe.7G205400_v2.0.a1
pyrus_communis	pycom02g06680 pycom02g06690
rosa_chinensis	RchiOBHm_Chr1g0330911 RchiOBHm_Chr2g0093881 RchiOBHm_Chr2g0094001 RchiOBHm_Chr7g0232521
rosa_laevigata	RLG00000001329 RLG00000016442 RLG00000016444
rosa_multiflora	Rmu_sc0003027.1_g000005 Rmu_sc0017523.1_g000016 Rmu_ssc0000369.1_g000008
rosa_roxburghii	Rroxscaffold_2G00147570 Rroxscaffold_2G00147700
rosa_rugosa	Rorug02G0040200 Rorug02G0040300
rosa_samantha	Rh2AG086900 Rh2AG087700 Rh2BG087700 Rh2CG090000 Rh2DG085600 Rh2DG086700 Rh2DG086800 Rh3CG371600 Rh7CG448000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	8
AccI	GTMKAC	2 cut(s)	533, 1109
AccII	CGCG	2 cut(s)	54, 682
AciI	CCGC	6 cut(s)	14, 20, 30, 68, 1459, 2277
AclWI	GGATC	5 cut(s)	720, 1715, 1728, 1977, 2015
AcoI	YGGCCR	2 cut(s)	90, 1895
AcsI	RAATTY	3 cut(s)	689, 1124, 1341
AcuI	CTGAAG	6 cut(s)	413, 690, 1017, 2001, 2069, 2183
AcvI	CACGTG	1 cut(s)	2261
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	9 cut(s)	224, 408, 517, 866, 891, 1160, 1682, 1705, 2138
AfiI	CCNNNNNNNGG	6 cut(s)	63, 169, 308, 904, 1400, 1461
AflIII	ACRYGT	2 cut(s)	680, 2260
AhlI	ACTAGT	1 cut(s)	2005
AjiI	CACGTC	1 cut(s)	115
AjnI	CCWGG	5 cut(s)	383, 1022, 1407, 1422, 1582
Alw21I	GWGCWC	2 cut(s)	433, 637
Alw26I	GTCTC	2 cut(s)	497, 1205
AlwI	GGATC	5 cut(s)	720, 1715, 1728, 1977, 2015
Ama87I	CYCGRG	2 cut(s)	434, 1612
AoxI	GGCC	3 cut(s)	90, 1020, 1895
ApeKI	GCWGC	3 cut(s)	956, 992, 1004
ApoI	RAATTY	3 cut(s)	689, 1124, 1341
Asp700I	GAANNNNTTC	2 cut(s)	130, 592
AspLEI	GCGC	1 cut(s)	56
AspS9I	GGNCC	3 cut(s)	109, 398, 907
AsuC2I	CCSGG	3 cut(s)	164, 1274, 2180
AsuHPI	GGTGA	3 cut(s)	178, 1238, 2194
AsuII	TTCGAA	1 cut(s)	461
AvaI	CYCGRG	2 cut(s)	434, 1612
AvaII	GGWCC	3 cut(s)	109, 398, 907
BalI	TGGCCA	1 cut(s)	1897
BamHI	GGATCC	1 cut(s)	1720
BanII	GRGCYC	1 cut(s)	362
BarI	GAAGNNNNNNTAC	4 cut(s)	206, 238, 873, 905
BbrPI	CACGTG	1 cut(s)	2261
Bbv12I	GWGCWC	2 cut(s)	433, 637
BbvI	GCAGC	3 cut(s)	943, 979, 1016
BccI	CCATC	3 cut(s)	154, 1483, 2114
BceAI	ACGGC	4 cut(s)	41, 43, 105, 1823
BciT130I	CCWGG	5 cut(s)	385, 1024, 1409, 1424, 1584
BciVI	GTATCC	2 cut(s)	619, 2185
BclI	TGATCA	1 cut(s)	1264
BcnI	CCSGG	3 cut(s)	164, 1274, 2180
BcoDI	GTCTC	2 cut(s)	497, 1205
BcuI	ACTAGT	1 cut(s)	2005
BfaI	CTAG	9 cut(s)	176, 489, 513, 537, 605, 705, 1901, 2006, 2025
BfmI	CTRYAG	1 cut(s)	1171
BfuI	GTATCC	2 cut(s)	619, 2185
BglII	AGATCT	1 cut(s)	1573
BisI	GCNGC	4 cut(s)	31, 957, 993, 1005
BlsI	GCNGC	4 cut(s)	32, 958, 994, 1006
BmcAI	AGTACT	1 cut(s)	517
Bme1390I	CCNGG	8 cut(s)	164, 385, 1024, 1274, 1409, 1424, 1584, 2180
Bme18I	GGWCC	3 cut(s)	109, 398, 907
BmeT110I	CYCGRG	2 cut(s)	434, 1612
BmgBI	CACGTC	1 cut(s)	115
BmgT120I	GGNCC	3 cut(s)	109, 398, 907
BmiI	GGNNCC	4 cut(s)	300, 399, 1387, 1722
BmrFI	CCNGG	8 cut(s)	164, 385, 1024, 1274, 1409, 1424, 1584, 2180
BmsI	GCATC	7 cut(s)	168, 1123, 1291, 1576, 1819, 1943, 2044
Bpu14I	TTCGAA	1 cut(s)	461
BpuEI	CTTGAG	1 cut(s)	983
BpuMI	CCSGG	3 cut(s)	164, 1274, 2180
BsaAI	YACGTR	3 cut(s)	836, 1717, 2261
BsaHI	GRCGYC	1 cut(s)	5
BsaJI	CCNNGG	5 cut(s)	87, 748, 1272, 1484, 2178
BsaWI	WCCGGW	1 cut(s)	1966
BsaXI	ACNNNNNCTCC	4 cut(s)	1156, 1186, 2245, 2275
Bsc4I	CCNNNNNNNGG	6 cut(s)	63, 169, 308, 904, 1400, 1461
Bse1I	ACTGG	4 cut(s)	589, 1602, 1744, 1991
Bse3DI	GCAATG	1 cut(s)	2228
BseBI	CCWGG	5 cut(s)	385, 1024, 1409, 1424, 1584
BseDI	CCNNGG	5 cut(s)	87, 748, 1272, 1484, 2178
BseGI	GGATG	4 cut(s)	159, 298, 1360, 1810
BseLI	CCNNNNNNNGG	6 cut(s)	63, 169, 308, 904, 1400, 1461
BseMI	GCAATG	1 cut(s)	2228
BseMII	CTCAG	2 cut(s)	344, 1221
BseNI	ACTGG	4 cut(s)	589, 1602, 1744, 1991
BseRI	GAGGAG	5 cut(s)	242, 555, 1052, 1334, 1928
BseX3I	CGGCCG	1 cut(s)	90
BseXI	GCAGC	3 cut(s)	943, 979, 1016
BsgI	GTGCAG	3 cut(s)	833, 858, 1276
Bsh1236I	CGCG	2 cut(s)	54, 682
Bsh1285I	CGRYCG	2 cut(s)	93, 97
BshFI	GGCC	3 cut(s)	92, 1022, 1897
BsiEI	CGRYCG	2 cut(s)	93, 97
BsiHKAI	GWGCWC	2 cut(s)	433, 637
BsiHKCI	CYCGRG	2 cut(s)	434, 1612
BsiSI	CCGG	5 cut(s)	164, 1274, 1462, 1967, 2180
BslFI	GGGAC	2 cut(s)	411, 1640
BslI	CCNNNNNNNGG	6 cut(s)	63, 169, 308, 904, 1400, 1461
BsmAI	GTCTC	2 cut(s)	497, 1205
BsmFI	GGGAC	2 cut(s)	411, 1640
BsmI	GAATGC	3 cut(s)	932, 958, 2203
BsnI	GGCC	3 cut(s)	92, 1022, 1897
BsoBI	CYCGRG	2 cut(s)	434, 1612
Bsp119I	TTCGAA	1 cut(s)	461
Bsp1286I	GDGCHC	3 cut(s)	362, 433, 637
Bsp1407I	TGTACA	1 cut(s)	864
Bsp143I	GATC	9 cut(s)	94, 154, 712, 1264, 1398, 1573, 1720, 1969, 2020
Bsp19I	CCATGG	1 cut(s)	748
BspACI	CCGC	6 cut(s)	14, 20, 30, 68, 1459, 2277
BspANI	GGCC	3 cut(s)	92, 1022, 1897
BspCNI	CTCAG	2 cut(s)	343, 1220
BspFNI	CGCG	2 cut(s)	54, 682
BspLI	GGNNCC	4 cut(s)	300, 399, 1387, 1722
BspPI	GGATC	5 cut(s)	720, 1715, 1728, 1977, 2015
BspQI	GCTCTTC	1 cut(s)	876
BspT104I	TTCGAA	1 cut(s)	461
BsrDI	GCAATG	1 cut(s)	2228
BsrGI	TGTACA	1 cut(s)	864
BsrI	ACTGG	4 cut(s)	589, 1602, 1744, 1991
BssECI	CCNNGG	5 cut(s)	87, 748, 1272, 1484, 2178
BssMI	GATC	9 cut(s)	94, 154, 712, 1264, 1398, 1573, 1720, 1969, 2020
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	2 cut(s)	748, 1484
Bst2UI	CCWGG	5 cut(s)	385, 1024, 1409, 1424, 1584
Bst4CI	ACNGT	6 cut(s)	415, 616, 639, 1172, 1492, 1708
Bst6I	CTCTTC	5 cut(s)	735, 876, 940, 993, 1145
BstACI	GRCGYC	1 cut(s)	5
BstAUI	TGTACA	1 cut(s)	864
BstBAI	YACGTR	3 cut(s)	836, 1717, 2261
BstBI	TTCGAA	1 cut(s)	461
BstC8I	GCNNGC	2 cut(s)	1056, 1530
BstDEI	CTNAG	9 cut(s)	317, 330, 653, 662, 1207, 1346, 1949, 2031, 2192
BstDSI	CCRYGG	2 cut(s)	87, 748
BstF5I	GGATG	4 cut(s)	159, 298, 1360, 1810
BstFNI	CGCG	2 cut(s)	54, 682
BstHHI	GCGC	1 cut(s)	56
BstKTI	GATC	9 cut(s)	97, 157, 715, 1267, 1401, 1576, 1723, 1972, 2023
BstMAI	GTCTC	2 cut(s)	497, 1205
BstMBI	GATC	9 cut(s)	94, 154, 712, 1264, 1398, 1573, 1720, 1969, 2020
BstMCI	CGRYCG	2 cut(s)	93, 97
BstMWI	GCNNNNNNNGC	4 cut(s)	1001, 1064, 1928, 2150
BstNI	CCWGG	5 cut(s)	385, 1024, 1409, 1424, 1584
BstNSI	RCATGY	2 cut(s)	865, 896
BstSCI	CCNGG	8 cut(s)	162, 383, 1022, 1272, 1407, 1422, 1582, 2178
BstSFI	CTRYAG	1 cut(s)	1171
BstUI	CGCG	2 cut(s)	54, 682
BstV1I	GCAGC	3 cut(s)	943, 979, 1016
BstX2I	RGATCY	3 cut(s)	1573, 1720, 1969
BstYI	RGATCY	3 cut(s)	1573, 1720, 1969
BstZI	CGGCCG	1 cut(s)	90
BsuI	GTATCC	2 cut(s)	619, 2185
BsuRI	GGCC	3 cut(s)	92, 1022, 1897
BtgI	CCRYGG	2 cut(s)	87, 748
BtrI	CACGTC	1 cut(s)	115
BtsCI	GGATG	4 cut(s)	159, 298, 1360, 1810
BtsIMutI	CAGTG	2 cut(s)	15, 1713
Cac8I	GCNNGC	2 cut(s)	1056, 1530
CfoI	GCGC	1 cut(s)	56
Cfr13I	GGNCC	3 cut(s)	109, 398, 907
CseI	GACGC	1 cut(s)	60
CsiI	ACCWGGT	1 cut(s)	1582
Csp6I	GTAC	9 cut(s)	223, 407, 516, 865, 890, 1159, 1681, 1704, 2137
CspCI	CAANNNNNGTGG	2 cut(s)	2054, 2089
CviAII	CATG	9 cut(s)	82, 118, 211, 452, 749, 862, 893, 1146, 2154
CviQI	GTAC	9 cut(s)	223, 407, 516, 865, 890, 1159, 1681, 1704, 2137
DdeI	CTNAG	9 cut(s)	317, 330, 653, 662, 1207, 1346, 1949, 2031, 2192
DpnI	GATC	9 cut(s)	96, 156, 714, 1266, 1400, 1575, 1722, 1971, 2022
DpnII	GATC	9 cut(s)	94, 154, 712, 1264, 1398, 1573, 1720, 1969, 2020
EaeI	YGGCCR	2 cut(s)	90, 1895
EagI	CGGCCG	1 cut(s)	90
Eam1104I	CTCTTC	5 cut(s)	735, 876, 940, 993, 1145
EarI	CTCTTC	5 cut(s)	735, 876, 940, 993, 1145
EclXI	CGGCCG	1 cut(s)	90
Eco130I	CCWWGG	2 cut(s)	748, 1484
Eco24I	GRGCYC	1 cut(s)	362
Eco32I	GATATC	2 cut(s)	340, 568
Eco47I	GGWCC	3 cut(s)	109, 398, 907
Eco52I	CGGCCG	1 cut(s)	90
Eco57I	CTGAAG	6 cut(s)	413, 690, 1017, 2001, 2069, 2183
Eco72I	CACGTG	1 cut(s)	2261
Eco88I	CYCGRG	2 cut(s)	434, 1612
EcoRII	CCWGG	5 cut(s)	383, 1022, 1407, 1422, 1582
EcoRV	GATATC	2 cut(s)	340, 568
EcoT14I	CCWWGG	2 cut(s)	748, 1484
EcoT38I	GRGCYC	1 cut(s)	362
ErhI	CCWWGG	2 cut(s)	748, 1484
FaeI	CATG	9 cut(s)	85, 121, 214, 455, 752, 865, 896, 1149, 2157
FalI	AAGNNNNNCTT	6 cut(s)	580, 612, 1038, 1070, 2142, 2174
FaqI	GGGAC	2 cut(s)	411, 1640
FatI	CATG	9 cut(s)	81, 117, 210, 451, 748, 861, 892, 1145, 2153
FauNDI	CATATG	1 cut(s)	808
FbaI	TGATCA	1 cut(s)	1264
FblI	GTMKAC	2 cut(s)	533, 1109
Fnu4HI	GCNGC	4 cut(s)	31, 957, 993, 1005
FokI	GGATG	4 cut(s)	146, 305, 1347, 1797
FriOI	GRGCYC	1 cut(s)	362
Fsp4HI	GCNGC	4 cut(s)	31, 957, 993, 1005
FspBI	CTAG	9 cut(s)	176, 489, 513, 537, 605, 705, 1901, 2006, 2025
GlaI	GCGC	1 cut(s)	55
GluI	GCNGC	4 cut(s)	31, 957, 993, 1005
HaeIII	GGCC	3 cut(s)	92, 1022, 1897
HapII	CCGG	5 cut(s)	164, 1274, 1462, 1967, 2180
HgaI	GACGC	1 cut(s)	60
HhaI	GCGC	1 cut(s)	56
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	9 cut(s)	85, 121, 214, 455, 752, 865, 896, 1149, 2157
Hin6I	GCGC	1 cut(s)	54
HinP1I	GCGC	1 cut(s)	54
HincII	GTYRAC	3 cut(s)	25, 1110, 2214
HindII	GTYRAC	3 cut(s)	25, 1110, 2214
HindIII	AAGCTT	2 cut(s)	319, 1946
HinfI	GANTC	8 cut(s)	76, 126, 313, 509, 724, 1106, 1688, 1880
HpaI	GTTAAC	1 cut(s)	2214
HpaII	CCGG	5 cut(s)	164, 1274, 1462, 1967, 2180
HphI	GGTGA	3 cut(s)	178, 1238, 2194
Hpy166II	GTNNAC	6 cut(s)	25, 112, 534, 1110, 1249, 2214
Hpy8I	GTNNAC	6 cut(s)	25, 112, 534, 1110, 1249, 2214
Hpy99I	CGWCG	3 cut(s)	38, 54, 199
HpyAV	CCTTC	9 cut(s)	199, 208, 262, 388, 617, 1012, 1171, 1223, 1309
HpyCH4III	ACNGT	6 cut(s)	415, 616, 639, 1172, 1492, 1708
HpyCH4IV	ACGT	7 cut(s)	5, 114, 197, 835, 1629, 1716, 2260
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1001, 1064, 1928, 2150
HpyF3I	CTNAG	9 cut(s)	317, 330, 653, 662, 1207, 1346, 1949, 2031, 2192
HpySE526I	ACGT	7 cut(s)	5, 114, 197, 835, 1629, 1716, 2260
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	9 cut(s)	85, 121, 214, 455, 752, 865, 896, 1149, 2157
HspAI	GCGC	1 cut(s)	54
Ksp22I	TGATCA	1 cut(s)	1264
KspAI	GTTAAC	1 cut(s)	2214
Kzo9I	GATC	9 cut(s)	94, 154, 712, 1264, 1398, 1573, 1720, 1969, 2020
LguI	GCTCTTC	1 cut(s)	876
LmnI	GCTCC	5 cut(s)	172, 436, 632, 1385, 1434
Lsp1109I	GCAGC	3 cut(s)	943, 979, 1016
LweI	GCATC	7 cut(s)	168, 1123, 1291, 1576, 1819, 1943, 2044
MabI	ACCWGGT	1 cut(s)	1582
MaeI	CTAG	9 cut(s)	176, 489, 513, 537, 605, 705, 1901, 2006, 2025
MaeII	ACGT	7 cut(s)	5, 114, 197, 835, 1629, 1716, 2260
MaeIII	GTNAC	5 cut(s)	166, 1148, 1885, 2182, 2256
MalI	GATC	9 cut(s)	96, 156, 714, 1266, 1400, 1575, 1722, 1971, 2022
MboI	GATC	9 cut(s)	94, 154, 712, 1264, 1398, 1573, 1720, 1969, 2020
MfeI	CAATTG	2 cut(s)	343, 1845
MflI	RGATCY	3 cut(s)	1573, 1720, 1969
MhlI	GDGCHC	3 cut(s)	362, 433, 637
MlsI	TGGCCA	1 cut(s)	1897
MluI	ACGCGT	1 cut(s)	680
MluNI	TGGCCA	1 cut(s)	1897
MlyI	GAGTC	3 cut(s)	518, 1115, 1889
MmeI	TCCRAC	2 cut(s)	164, 934
Mox20I	TGGCCA	1 cut(s)	1897
MroXI	GAANNNNTTC	2 cut(s)	130, 592
MscI	TGGCCA	1 cut(s)	1897
MseI	TTAA	4 cut(s)	618, 1284, 1934, 2213
MslI	CAYNNNNRTG	2 cut(s)	164, 2052
Msp20I	TGGCCA	1 cut(s)	1897
MspI	CCGG	5 cut(s)	164, 1274, 1462, 1967, 2180
MspR9I	CCNGG	8 cut(s)	164, 385, 1024, 1274, 1409, 1424, 1584, 2180
MunI	CAATTG	2 cut(s)	343, 1845
Mva1269I	GAATGC	3 cut(s)	932, 958, 2203
MvaI	CCWGG	5 cut(s)	385, 1024, 1409, 1424, 1584
MvnI	CGCG	2 cut(s)	54, 682
MwoI	GCNNNNNNNGC	4 cut(s)	1001, 1064, 1928, 2150
NciI	CCSGG	3 cut(s)	164, 1274, 2180
NcoI	CCATGG	1 cut(s)	748
NdeI	CATATG	1 cut(s)	808
NdeII	GATC	9 cut(s)	94, 154, 712, 1264, 1398, 1573, 1720, 1969, 2020
NlaIII	CATG	9 cut(s)	85, 121, 214, 455, 752, 865, 896, 1149, 2157
NlaIV	GGNNCC	4 cut(s)	300, 399, 1387, 1722
NmuCI	GTSAC	5 cut(s)	166, 1148, 1885, 2182, 2256
NspI	RCATGY	2 cut(s)	865, 896
NspV	TTCGAA	1 cut(s)	461
PaeR7I	CTCGAG	1 cut(s)	1612
PciSI	GCTCTTC	1 cut(s)	876
PctI	GAATGC	3 cut(s)	932, 958, 2203
PdmI	GAANNNNTTC	2 cut(s)	130, 592
PfeI	GAWTC	5 cut(s)	76, 126, 313, 724, 1688
PkrI	GCNGC	4 cut(s)	32, 958, 994, 1006
Ple19I	CGATCG	1 cut(s)	97
PleI	GAGTC	3 cut(s)	517, 1114, 1888
PmaCI	CACGTG	1 cut(s)	2261
PmlI	CACGTG	1 cut(s)	2261
PpsI	GAGTC	3 cut(s)	517, 1114, 1888
Ppu21I	YACGTR	3 cut(s)	836, 1717, 2261
Psp6I	CCWGG	5 cut(s)	383, 1022, 1407, 1422, 1582
PspCI	CACGTG	1 cut(s)	2261
PspGI	CCWGG	5 cut(s)	383, 1022, 1407, 1422, 1582
PspN4I	GGNNCC	4 cut(s)	300, 399, 1387, 1722
PspPI	GGNCC	3 cut(s)	109, 398, 907
PspXI	VCTCGAGB	1 cut(s)	1612
PsuI	RGATCY	3 cut(s)	1573, 1720, 1969
PvuI	CGATCG	1 cut(s)	97
RsaI	GTAC	9 cut(s)	224, 408, 517, 866, 891, 1160, 1682, 1705, 2138
RsaNI	GTAC	9 cut(s)	223, 407, 516, 865, 890, 1159, 1681, 1704, 2137
RseI	CAYNNNNRTG	2 cut(s)	164, 2052
SalI	GTCGAC	1 cut(s)	1108
SapI	GCTCTTC	1 cut(s)	876
SaqAI	TTAA	4 cut(s)	618, 1284, 1934, 2213
SatI	GCNGC	4 cut(s)	31, 957, 993, 1005
Sau3AI	GATC	9 cut(s)	94, 154, 712, 1264, 1398, 1573, 1720, 1969, 2020
Sau96I	GGNCC	3 cut(s)	109, 398, 907
ScaI	AGTACT	1 cut(s)	517
SchI	GAGTC	3 cut(s)	518, 1115, 1889
ScrFI	CCNGG	8 cut(s)	164, 385, 1024, 1274, 1409, 1424, 1584, 2180
SduI	GDGCHC	3 cut(s)	362, 433, 637
SexAI	ACCWGGT	1 cut(s)	1582
SfaNI	GCATC	7 cut(s)	168, 1123, 1291, 1576, 1819, 1943, 2044
SfcI	CTRYAG	1 cut(s)	1171
Sfr274I	CTCGAG	1 cut(s)	1612
SfuI	TTCGAA	1 cut(s)	461
SinI	GGWCC	3 cut(s)	109, 398, 907
SlaI	CTCGAG	1 cut(s)	1612
SmiMI	CAYNNNNRTG	2 cut(s)	164, 2052
SmlI	CTYRAG	2 cut(s)	962, 1612
SmoI	CTYRAG	2 cut(s)	962, 1612
SpeI	ACTAGT	1 cut(s)	2005
SsiI	CCGC	6 cut(s)	14, 20, 30, 68, 1459, 2277
SspMI	CTAG	9 cut(s)	176, 489, 513, 537, 605, 705, 1901, 2006, 2025
StyD4I	CCNGG	8 cut(s)	162, 383, 1022, 1272, 1407, 1422, 1582, 2178
StyI	CCWWGG	2 cut(s)	748, 1484
TaaI	ACNGT	6 cut(s)	415, 616, 639, 1172, 1492, 1708
TaiI	ACGT	7 cut(s)	8, 117, 200, 838, 1632, 1719, 2263
TaqI	TCGA	6 cut(s)	461, 798, 1109, 1294, 1570, 1613
TatI	WGTACW	4 cut(s)	515, 864, 1680, 2136
TauI	GCSGC	1 cut(s)	33
TfiI	GAWTC	5 cut(s)	76, 126, 313, 724, 1688
Tru1I	TTAA	4 cut(s)	618, 1284, 1934, 2213
Tru9I	TTAA	4 cut(s)	618, 1284, 1934, 2213
TscAI	CASTG	2 cut(s)	15, 1713
TseFI	GTSAC	5 cut(s)	166, 1148, 1885, 2182, 2256
TseI	GCWGC	3 cut(s)	956, 992, 1004
Tsp45I	GTSAC	5 cut(s)	166, 1148, 1885, 2182, 2256
TspDTI	ATGAA	5 cut(s)	227, 440, 588, 636, 1431
TspGWI	ACGGA	5 cut(s)	51, 57, 185, 592, 1691
TspRI	CASTG	2 cut(s)	15, 1713
VpaK11BI	GGWCC	3 cut(s)	109, 398, 907
XapI	RAATTY	3 cut(s)	689, 1124, 1341
XceI	RCATGY	2 cut(s)	865, 896
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1463, 1482
XhoI	CTCGAG	1 cut(s)	1612
XmiI	GTMKAC	2 cut(s)	533, 1109
XmnI	GAANNNNTTC	2 cut(s)	130, 592
XspI	CTAG	9 cut(s)	176, 489, 513, 537, 605, 705, 1901, 2006, 2025
ZraI	GACGTC	1 cut(s)	6
ZrmI	AGTACT	1 cut(s)	517

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.