Rh7CG448000

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0002207
Physical Location & Seq
Reverse (-)
58918933 .. 58930877
11945 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG448000.1

Sequence Viewer

Length: 636 bp
ATGGAACACCGAACACAGCAACCTGCAAGGCTACAACCAGCAACCCGTCTCCGCCAGTCCGCCTCTGCAGTCTCCGAGCTCCGCCTCCAAGCTCCAGGCATTGCCCTCTGTCTCGACCTCTCAAGCTTTATAAAGCTTCAATATTTTGAGTTCCCTTCTTCCTCGCCCAACAATAGAACTAGTGGAGTCATTGAAAGCATAGCTTGTGCCCTATCAAAGTGGAAGAAGGGAAGGCTTCTTGGAAGAGGGACTTTTGGTCATGTTTATGTCGGATTTAACAGGTACAGGAGATACAGTAGGAAGGAAATAGAGATAGCAACAAACTTCTTCTCAGAGAGTAATGTGATTGGTGAAGGAGGATATGGGAAAGTTTACAAGTGCAGTCTTGATCAAACCCCGGTAGCTGTCAAGGTTCTTCGACCTGATGCAGTTGAGAAGAAAGAGGAGTTTCTGAAAGAGGAAATCATGGACGACCCACACATTTCTGCCGATGGCTTTACTTATGAGTACAGAGCAATCAAAGCATGGCTTGACAAACACAACGTATCCCCAGTGACAAGACTTAGACTTCAGCATTCAGAGTTAACTCCTAACCATACATTGCGTTCAGCCATACAAGAATGGGGTCACGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr U-box Usp

Protein Analysis

211

Amino Acids

23.98

Weight (kDa)

9.36

Isoelectric Point (pI)

54.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 152 - 208 2.5e-14 U-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 131
Acc36I ACCTGC 1 cut(s) 31
AccII CGCG 1 cut(s) 632
AciI CCGC 3 cut(s) 52, 60, 82
AcuI CTGAAG 1 cut(s) 554
AfaI GTAC 2 cut(s) 284, 509
AflIII ACRYGT 1 cut(s) 630
AgsI TTSAA 2 cut(s) 140, 194
AhdI GACNNNNNGTC 1 cut(s) 255
AhlI ACTAGT 1 cut(s) 179
AjnI CCWGG 1 cut(s) 94
AluBI AGCT 6 cut(s) 79, 92, 126, 136, 203, 404
AluI AGCT 6 cut(s) 79, 92, 126, 136, 203, 404
Alw21I GWGCWC 1 cut(s) 81
Alw26I GTCTC 3 cut(s) 53, 76, 116
AsuC2I CCSGG 1 cut(s) 398
AsuHPI GGTGA 1 cut(s) 362
BaeGI GKGCMC 1 cut(s) 211
BanII GRGCYC 1 cut(s) 81
Bbv12I GWGCWC 1 cut(s) 81
BccI CCATC 1 cut(s) 485
BciT130I CCWGG 1 cut(s) 96
BciVI GTATCC 1 cut(s) 556
BclI TGATCA 1 cut(s) 388
BcnI CCSGG 1 cut(s) 398
BcoDI GTCTC 3 cut(s) 53, 76, 116
BcuI ACTAGT 1 cut(s) 179
BfaI CTAG 1 cut(s) 180
BfmI CTRYAG 1 cut(s) 66
BfuAI ACCTGC 1 cut(s) 31
BfuI GTATCC 1 cut(s) 556
Bme1390I CCNGG 2 cut(s) 96, 398
BmeRI GACNNNNNGTC 1 cut(s) 255
BmrFI CCNGG 2 cut(s) 96, 398
BmrI ACTGGG 1 cut(s) 545
BmsI GCATC 1 cut(s) 415
BmuI ACTGGG 1 cut(s) 545
BpmI CTGGAG 1 cut(s) 78
BpuEI CTTGAG 1 cut(s) 106
BpuMI CCSGG 1 cut(s) 398
BsaJI CCNNGG 1 cut(s) 396
BsaXI ACNNNNNCTCC 2 cut(s) 280, 310
Bse1I ACTGG 2 cut(s) 55, 551
Bse3DI GCAATG 2 cut(s) 99, 599
BseBI CCWGG 1 cut(s) 96
BseDI CCNNGG 1 cut(s) 396
BseMI GCAATG 2 cut(s) 99, 599
BseMII CTCAG 1 cut(s) 345
BseNI ACTGG 2 cut(s) 55, 551
BseRI GAGGAG 1 cut(s) 458
BseSI GKGCMC 1 cut(s) 211
BsgI GTGCAG 1 cut(s) 400
Bsh1236I CGCG 1 cut(s) 632
BsiHKAI GWGCWC 1 cut(s) 81
BsiSI CCGG 1 cut(s) 398
BslFI GGGAC 1 cut(s) 262
BsmAI GTCTC 3 cut(s) 53, 76, 116
BsmBI CGTCTC 1 cut(s) 53
BsmFI GGGAC 1 cut(s) 262
BsmI GAATGC 1 cut(s) 574
Bsp1286I GDGCHC 2 cut(s) 81, 211
Bsp143I GATC 1 cut(s) 388
BspACI CCGC 3 cut(s) 52, 60, 82
BspCNI CTCAG 1 cut(s) 344
BspFNI CGCG 1 cut(s) 632
BspMAI CTGCAG 1 cut(s) 70
BspMI ACCTGC 1 cut(s) 31
BsrDI GCAATG 2 cut(s) 99, 599
BsrI ACTGG 2 cut(s) 55, 551
BssECI CCNNGG 1 cut(s) 396
BssMI GATC 1 cut(s) 388
Bst2UI CCWGG 1 cut(s) 96
Bst4CI ACNGT 1 cut(s) 296
Bst6I CTCTTC 1 cut(s) 238
BstDEI CTNAG 2 cut(s) 331, 563
BstFNI CGCG 1 cut(s) 632
BstKTI GATC 1 cut(s) 391
BstMAI GTCTC 3 cut(s) 53, 76, 116
BstMBI GATC 1 cut(s) 388
BstMWI GCNNNNNNNGC 1 cut(s) 521
BstNI CCWGG 1 cut(s) 96
BstSCI CCNGG 2 cut(s) 94, 396
BstSFI CTRYAG 1 cut(s) 66
BstSLI GKGCMC 1 cut(s) 211
BstUI CGCG 1 cut(s) 632
BsuI GTATCC 1 cut(s) 556
BtsIMutI CAGTG 1 cut(s) 558
BveI ACCTGC 1 cut(s) 31
Csp6I GTAC 2 cut(s) 283, 508
CviAII CATG 3 cut(s) 260, 466, 525
CviQI GTAC 2 cut(s) 283, 508
DdeI CTNAG 2 cut(s) 331, 563
DpnI GATC 1 cut(s) 390
DpnII GATC 1 cut(s) 388
DriI GACNNNNNGTC 1 cut(s) 255
Eam1104I CTCTTC 1 cut(s) 238
Eam1105I GACNNNNNGTC 1 cut(s) 255
EarI CTCTTC 1 cut(s) 238
EciI GGCGGA 3 cut(s) 41, 49, 71
Ecl136II GAGCTC 1 cut(s) 79
Eco24I GRGCYC 1 cut(s) 81
Eco53kI GAGCTC 1 cut(s) 79
Eco57I CTGAAG 1 cut(s) 554
EcoICRI GAGCTC 1 cut(s) 79
EcoRII CCWGG 1 cut(s) 94
EcoT38I GRGCYC 1 cut(s) 81
Esp3I CGTCTC 1 cut(s) 53
FaeI CATG 3 cut(s) 263, 469, 528
FalI AAGNNNNNCTT 6 cut(s) 187, 219, 235, 267, 513, 545
FaqI GGGAC 1 cut(s) 262
FatI CATG 3 cut(s) 259, 465, 524
FbaI TGATCA 1 cut(s) 388
FriOI GRGCYC 1 cut(s) 81
FspBI CTAG 1 cut(s) 180
GsuI CTGGAG 1 cut(s) 78
HapII CCGG 1 cut(s) 398
Hin1II CATG 3 cut(s) 263, 469, 528
HincII GTYRAC 1 cut(s) 585
HindII GTYRAC 1 cut(s) 585
HindIII AAGCTT 2 cut(s) 124, 134
HinfI GANTC 1 cut(s) 186
HpaI GTTAAC 1 cut(s) 585
HpaII CCGG 1 cut(s) 398
HphI GGTGA 1 cut(s) 362
Hpy166II GTNNAC 2 cut(s) 373, 585
Hpy188I TCNGA 5 cut(s) 76, 272, 334, 453, 580
Hpy188III TCNNGA 2 cut(s) 113, 386
Hpy8I GTNNAC 2 cut(s) 373, 585
HpyAV CCTTC 5 cut(s) 165, 220, 225, 295, 347
HpyCH4III ACNGT 1 cut(s) 296
HpyCH4IV ACGT 1 cut(s) 543
HpyCH4V TGCA 4 cut(s) 26, 68, 381, 428
HpyF10VI GCNNNNNNNGC 1 cut(s) 521
HpyF3I CTNAG 2 cut(s) 331, 563
HpySE526I ACGT 1 cut(s) 543
Hsp92II CATG 3 cut(s) 263, 469, 528
Ksp22I TGATCA 1 cut(s) 388
KspAI GTTAAC 1 cut(s) 585
Kzo9I GATC 1 cut(s) 388
LmnI GCTCC 2 cut(s) 84, 97
LweI GCATC 1 cut(s) 415
MaeI CTAG 1 cut(s) 180
MaeII ACGT 1 cut(s) 543
MaeIII GTNAC 2 cut(s) 553, 626
MalI GATC 1 cut(s) 390
MboI GATC 1 cut(s) 388
MboII GAAGA 6 cut(s) 150, 235, 255, 319, 407, 448
MhlI GDGCHC 2 cut(s) 81, 211
MluI ACGCGT 1 cut(s) 630
MlyI GAGTC 1 cut(s) 195
MmeI TCCRAC 1 cut(s) 250
MnlI CCTC 9 cut(s) 73, 95, 116, 128, 172, 239, 350, 436, 451
MseI TTAA 2 cut(s) 276, 584
MslI CAYNNNNRTG 1 cut(s) 264
MspI CCGG 1 cut(s) 398
MspR9I CCNGG 2 cut(s) 96, 398
Mva1269I GAATGC 1 cut(s) 574
MvaI CCWGG 1 cut(s) 96
MvnI CGCG 1 cut(s) 632
MwoI GCNNNNNNNGC 1 cut(s) 521
NciI CCSGG 1 cut(s) 398
NdeII GATC 1 cut(s) 388
NlaIII CATG 3 cut(s) 263, 469, 528
NmuCI GTSAC 2 cut(s) 553, 626
PctI GAATGC 1 cut(s) 574
PleI GAGTC 1 cut(s) 194
PpsI GAGTC 1 cut(s) 194
PsiI TTATAA 1 cut(s) 131
Psp124BI GAGCTC 1 cut(s) 81
Psp6I CCWGG 1 cut(s) 94
PspGI CCWGG 1 cut(s) 94
PstI CTGCAG 1 cut(s) 70
RsaI GTAC 2 cut(s) 284, 509
RsaNI GTAC 2 cut(s) 283, 508
RseI CAYNNNNRTG 1 cut(s) 264
SacI GAGCTC 1 cut(s) 81
SaqAI TTAA 2 cut(s) 276, 584
Sau3AI GATC 1 cut(s) 388
SchI GAGTC 1 cut(s) 195
ScrFI CCNGG 2 cut(s) 96, 398
SduI GDGCHC 2 cut(s) 81, 211
SfaNI GCATC 1 cut(s) 415
SfcI CTRYAG 1 cut(s) 66
SmiMI CAYNNNNRTG 1 cut(s) 264
SmlI CTYRAG 1 cut(s) 121
SmoI CTYRAG 1 cut(s) 121
SpeI ACTAGT 1 cut(s) 179
SsiI CCGC 3 cut(s) 52, 60, 82
SspI AATATT 1 cut(s) 143
SspMI CTAG 1 cut(s) 180
SstI GAGCTC 1 cut(s) 81
StyD4I CCNGG 2 cut(s) 94, 396
TaaI ACNGT 1 cut(s) 296
TaiI ACGT 1 cut(s) 546
TaqI TCGA 2 cut(s) 114, 418
TatI WGTACW 1 cut(s) 507
Tru1I TTAA 2 cut(s) 276, 584
Tru9I TTAA 2 cut(s) 276, 584
TscAI CASTG 1 cut(s) 558
TseFI GTSAC 2 cut(s) 553, 626
Tsp45I GTSAC 2 cut(s) 553, 626
TspRI CASTG 1 cut(s) 558
XspI CTAG 1 cut(s) 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.