Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0001822

Physical Location & Seq

Genomic Coordinates Forward (+)

10632598 .. 10635040

Gene Structure 2443 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1132000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 972 bp

ATGGATCATTGGAAATCCGACGATTTCTCAGCTGCTTCGGCTCCTTCTTCTACTCAACCTCTCCCCTGCGGTCGCCCCCACAACTCCAACTCCGATTCCAGGCAAAGACTTGATGTAGAAACGAAGGACCCGATTGTTGCCAGAAAAGTTCAGAAAGCAGACCGCGAAAAGTTGAGGAGGGATCGACTCAATGAGCATTTTTTTGAGTTGGGCAACACATTAGATCCGGATAGGCCCAAGAACGATAAGGCAACCATCCTTACCGACACAATTCAAATGTTGAAGGATTTAACCACTGATGTCAACAAACTAAAGGCCGAGTGTTCTGCACTTACTGATGAATCCCGTGAGCTAACGCAGGAGAAGAACGAGCTAAGAGAGGAGAAGGCATCTTTAAAATCTGATATTGAAAATCTAAACGTTCAGTATCAGCAGAGACTGAGAGTGATGTTTCCATGGGCTGGCATGGATCCTTCTGTTGTTATGGCTCCACCTTACTCATATCCAATGCCTGTGGCTGTCCCTTCCGGCCCAATTCCCATGCACCCATCACTTCAGTCGTATCCTTACTTTCAAGCTCAAAATCCTGCTGCTATGCCCAATCCCTGTTCAACTTTTGTGCCGTATCCAGCACCTGCCAATCCTCCTGTACAACAGCCATCACCGCTGTATGCATCCATCTCCCACCTTTCAAGCAAGCAAGATTCCAGGAGCAGATCATCAGATCATCAAGGGGGTAGCACTGCCGAAAGGTGTGATGACTCCAACGATGTGGCAACAGACCTTGAACTTAAGATGCCAGGATCTTCAACACCACAGGATTCATCTTCTGCAGTTAAAAAGGCCAAGCAATCACAGAGGAAAGATAGGAGTGTTACAGATGGATGCTCATCAAGTAGGCATTCTACATCTCAAGTTGTTCAGGATAGCTCCTCCAACAGCGTAGGTGACGTCCCTAAGCCTAACAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0008150 GO:0009507 GO:0009536 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HLH

Protein Analysis

324

Amino Acids

35.7

Weight (kDa)

6.4

Isoelectric Point (pI)

65.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
bHLH_IRO3	PF23177	46 - 121	3.7e-20	Iron-related transcription factor 3 bHLH domain
HLH	PF00010	48 - 98	1.5e-09	Helix-loop-helix DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001822)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G19860 AT3G19860 AT3G19860
fragaria_vesca	FvH4_1g11770 FvH4_2g02000 FvH4_2g02000 FvH4_2g02000
malus_domestica	MD02G1132000.v1.1 MD05G1115800.v1.1 MD15G1245200.v1.1
prunus_persica	Prupe.7G168500_v2.0.a1 Prupe.7G168500_v2.0.a1 Prupe.7G168500_v2.0.a1 Prupe.7G168500_v2.0.a1 Prupe.7G168500_v2.0.a1 Prupe.7G168500_v2.0.a1 Prupe.8G160700_v2.0.a1
pyrus_communis	pycom02g10290 pycom05g11010 pycom15g21570
rosa_chinensis	RchiOBHm_Chr2g0099391 RchiOBHm_Chr6g0245181
rosa_laevigata	RLG00000015318 RLG00000016878
rosa_multiflora	Rmu_co8372605.1_g000001 Rmu_sc0001288.1_g000004 Rmu_sc0002487.1_g000008 Rmu_sc0027529.1_g000004
rosa_roxburghii	Rroxscaffold_2G00143110 Rroxscaffold_3G00242190 Rroxscaffold_7G00215380
rosa_rugosa	Rorug02G0079200 Rorug02G0079300 Rorug02G0079400 Rorug05G0512600 Rorug05G0512700 Rorug05G0512800.1
rosa_samantha	Rh2AG129100 Rh2BG132100 Rh2CG132900 Rh2DG133100 Rh6AG027700 Rh6BG023800 Rh6CG022200 Rh6DG022300
rosa_wichuraiana	Rw2G009940 Rw6G002340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	643
AatII	GACGTC	1 cut(s)	954
Acc36I	ACCTGC	1 cut(s)	643
AccB7I	CCANNNNNTGG	1 cut(s)	461
AccII	CGCG	1 cut(s)	165
AccIII	TCCGGA	1 cut(s)	226
AciI	CCGC	3 cut(s)	69, 163, 665
AclI	AACGTT	1 cut(s)	420
AclWI	GGATC	6 cut(s)	12, 189, 218, 464, 477, 811
AcuI	CTGAAG	1 cut(s)	539
AcyI	GRCGYC	1 cut(s)	951
AfaI	GTAC	1 cut(s)	651
AfiI	CCNNNNNNNGG	2 cut(s)	99, 461
AflII	CTTAAG	1 cut(s)	791
AgsI	TTSAA	8 cut(s)	275, 283, 410, 575, 612, 693, 788, 810
AjnI	CCWGG	3 cut(s)	98, 707, 799
AluBI	AGCT	5 cut(s)	32, 352, 373, 578, 930
AluI	AGCT	5 cut(s)	32, 352, 373, 578, 930
Alw26I	GTCTC	1 cut(s)	430
AlwI	GGATC	6 cut(s)	12, 189, 218, 464, 477, 811
AlwNI	CAGNNNCTG	2 cut(s)	439, 635
Aor13HI	TCCGGA	1 cut(s)	226
AoxI	GGCC	4 cut(s)	233, 315, 529, 843
ApeKI	GCWGC	2 cut(s)	32, 590
AspS9I	GGNCC	3 cut(s)	127, 234, 530
AsuHPI	GGTGA	2 cut(s)	654, 959
AvaII	GGWCC	1 cut(s)	127
BamHI	GGATCC	1 cut(s)	469
BbvI	GCAGC	2 cut(s)	19, 577
BccI	CCATC	5 cut(s)	263, 556, 667, 686, 875
BceAI	ACGGC	1 cut(s)	607
BcgI	CGANNNNNNTGC	2 cut(s)	308, 342
BciT130I	CCWGG	3 cut(s)	100, 709, 801
BciVI	GTATCC	2 cut(s)	573, 636
BcoDI	GTCTC	1 cut(s)	430
BfmI	CTRYAG	1 cut(s)	831
BfrI	CTTAAG	1 cut(s)	791
BfuAI	ACCTGC	1 cut(s)	643
BfuI	GTATCC	2 cut(s)	573, 636
BisI	GCNGC	2 cut(s)	33, 591
BlsI	GCNGC	2 cut(s)	34, 592
Bme1390I	CCNGG	3 cut(s)	100, 709, 801
Bme18I	GGWCC	1 cut(s)	127
BmgT120I	GGNCC	3 cut(s)	127, 234, 530
BmiI	GGNNCC	4 cut(s)	42, 129, 471, 489
BmrFI	CCNGG	3 cut(s)	100, 709, 801
BmsI	GCATC	4 cut(s)	398, 683, 786, 875
Bpu10I	CCTNAGC	1 cut(s)	957
BpuEI	CTTGAG	1 cut(s)	897
BsaBI	GATNNNNATC	1 cut(s)	889
BsaHI	GRCGYC	1 cut(s)	951
BsaJI	CCNNGG	1 cut(s)	455
BsaWI	WCCGGW	1 cut(s)	226
BsaXI	ACNNNNNCTCC	2 cut(s)	74, 104
Bsc4I	CCNNNNNNNGG	2 cut(s)	99, 461
Bse8I	GATNNNNATC	1 cut(s)	889
BseAI	TCCGGA	1 cut(s)	226
BseBI	CCWGG	3 cut(s)	100, 709, 801
BseDI	CCNNGG	1 cut(s)	455
BseGI	GGATG	3 cut(s)	255, 674, 890
BseJI	GATNNNNATC	1 cut(s)	889
BseLI	CCNNNNNNNGG	2 cut(s)	99, 461
BseMII	CTCAG	2 cut(s)	42, 431
BseRI	GAGGAG	3 cut(s)	190, 395, 922
BseXI	GCAGC	2 cut(s)	19, 577
BsgI	GTGCAG	1 cut(s)	312
Bsh1236I	CGCG	1 cut(s)	165
Bsh1285I	CGRYCG	1 cut(s)	73
BshFI	GGCC	4 cut(s)	235, 317, 531, 845
BsiEI	CGRYCG	1 cut(s)	73
BsiSI	CCGG	2 cut(s)	227, 528
BslFI	GGGAC	2 cut(s)	506, 938
BslI	CCNNNNNNNGG	2 cut(s)	99, 461
BsmAI	GTCTC	1 cut(s)	430
BsmFI	GGGAC	2 cut(s)	506, 938
BsmI	GAATGC	1 cut(s)	901
BsnI	GGCC	4 cut(s)	235, 317, 531, 845
Bsp13I	TCCGGA	1 cut(s)	226
Bsp1407I	TGTACA	1 cut(s)	649
Bsp143I	GATC	7 cut(s)	4, 181, 223, 469, 716, 724, 803
Bsp19I	CCATGG	1 cut(s)	455
BspACI	CCGC	3 cut(s)	69, 163, 665
BspANI	GGCC	4 cut(s)	235, 317, 531, 845
BspCNI	CTCAG	2 cut(s)	41, 432
BspEI	TCCGGA	1 cut(s)	226
BspFNI	CGCG	1 cut(s)	165
BspLI	GGNNCC	4 cut(s)	42, 129, 471, 489
BspMAI	CTGCAG	1 cut(s)	835
BspMI	ACCTGC	1 cut(s)	643
BspPI	GGATC	6 cut(s)	12, 189, 218, 464, 477, 811
BspTI	CTTAAG	1 cut(s)	791
BsrGI	TGTACA	1 cut(s)	649
BssECI	CCNNGG	1 cut(s)	455
BssMI	GATC	7 cut(s)	4, 181, 223, 469, 716, 724, 803
BssNI	GRCGYC	1 cut(s)	951
BssT1I	CCWWGG	1 cut(s)	455
Bst2UI	CCWGG	3 cut(s)	100, 709, 801
BstACI	GRCGYC	1 cut(s)	951
BstAFI	CTTAAG	1 cut(s)	791
BstAUI	TGTACA	1 cut(s)	649
BstC8I	GCNNGC	2 cut(s)	463, 698
BstDEI	CTNAG	4 cut(s)	28, 374, 440, 957
BstDSI	CCRYGG	1 cut(s)	455
BstF5I	GGATG	3 cut(s)	255, 674, 890
BstFNI	CGCG	1 cut(s)	165
BstKTI	GATC	7 cut(s)	7, 184, 226, 472, 719, 727, 806
BstMAI	GTCTC	1 cut(s)	430
BstMBI	GATC	7 cut(s)	4, 181, 223, 469, 716, 724, 803
BstMCI	CGRYCG	1 cut(s)	73
BstMWI	GCNNNNNNNGC	2 cut(s)	38, 664
BstNI	CCWGG	3 cut(s)	100, 709, 801
BstSCI	CCNGG	3 cut(s)	98, 707, 799
BstSFI	CTRYAG	1 cut(s)	831
BstUI	CGCG	1 cut(s)	165
BstV1I	GCAGC	2 cut(s)	19, 577
BstX2I	RGATCY	3 cut(s)	223, 469, 803
BstXI	CCANNNNNNTGG	1 cut(s)	772
BstYI	RGATCY	3 cut(s)	223, 469, 803
BsuI	GTATCC	2 cut(s)	573, 636
BsuRI	GGCC	4 cut(s)	235, 317, 531, 845
BtgI	CCRYGG	1 cut(s)	455
BtsCI	GGATG	3 cut(s)	255, 674, 890
BtsI	GCAGTG	1 cut(s)	741
BtsIMutI	CAGTG	2 cut(s)	294, 741
BveI	ACCTGC	1 cut(s)	643
Cac8I	GCNNGC	2 cut(s)	463, 698
CaiI	CAGNNNCTG	2 cut(s)	439, 635
Cfr13I	GGNCC	3 cut(s)	127, 234, 530
Csp6I	GTAC	1 cut(s)	650
CspCI	CAANNNNNGTGG	2 cut(s)	495, 530
CviAII	CATG	3 cut(s)	456, 466, 541
CviQI	GTAC	1 cut(s)	650
DdeI	CTNAG	4 cut(s)	28, 374, 440, 957
DpnI	GATC	7 cut(s)	6, 183, 225, 471, 718, 726, 805
DpnII	GATC	7 cut(s)	4, 181, 223, 469, 716, 724, 803
DraI	TTTAAA	1 cut(s)	396
Eco130I	CCWWGG	1 cut(s)	455
Eco47I	GGWCC	1 cut(s)	127
Eco57I	CTGAAG	1 cut(s)	539
EcoO109I	RGGNCCY	1 cut(s)	127
EcoRII	CCWGG	3 cut(s)	98, 707, 799
EcoT14I	CCWWGG	1 cut(s)	455
EcoT22I	ATGCAT	1 cut(s)	676
ErhI	CCWWGG	1 cut(s)	455
FaeI	CATG	3 cut(s)	459, 469, 544
FaiI	YATR	7 cut(s)	457, 467, 485, 502, 542, 596, 672
FaqI	GGGAC	2 cut(s)	506, 938
FatI	CATG	3 cut(s)	455, 465, 540
Fnu4HI	GCNGC	2 cut(s)	33, 591
FokI	GGATG	3 cut(s)	242, 661, 897
Fsp4HI	GCNGC	2 cut(s)	33, 591
GluI	GCNGC	2 cut(s)	33, 591
HaeIII	GGCC	4 cut(s)	235, 317, 531, 845
HapII	CCGG	2 cut(s)	227, 528
Hin1I	GRCGYC	1 cut(s)	951
Hin1II	CATG	3 cut(s)	459, 469, 544
HincII	GTYRAC	1 cut(s)	304
HindII	GTYRAC	1 cut(s)	304
HinfI	GANTC	6 cut(s)	95, 186, 341, 704, 761, 821
HpaII	CCGG	2 cut(s)	227, 528
HphI	GGTGA	2 cut(s)	654, 959
Hpy166II	GTNNAC	1 cut(s)	304
Hpy188I	TCNGA	5 cut(s)	19, 94, 153, 403, 724
Hpy188III	TCNNGA	2 cut(s)	227, 923
Hpy8I	GTNNAC	1 cut(s)	304
Hpy99I	CGWCG	1 cut(s)	23
HpyAV	CCTTC	6 cut(s)	54, 118, 277, 379, 483, 534
HpyCH4IV	ACGT	2 cut(s)	420, 951
HpyCH4V	TGCA	4 cut(s)	329, 544, 674, 833
HpyF10VI	GCNNNNNNNGC	2 cut(s)	38, 664
HpyF3I	CTNAG	4 cut(s)	28, 374, 440, 957
HpySE526I	ACGT	2 cut(s)	420, 951
Hsp92I	GRCGYC	1 cut(s)	951
Hsp92II	CATG	3 cut(s)	459, 469, 544
Kpn2I	TCCGGA	1 cut(s)	226
Kzo9I	GATC	7 cut(s)	4, 181, 223, 469, 716, 724, 803
LmnI	GCTCC	4 cut(s)	46, 493, 711, 935
Lsp1109I	GCAGC	2 cut(s)	19, 577
LweI	GCATC	4 cut(s)	398, 683, 786, 875
MaeII	ACGT	2 cut(s)	420, 951
MaeIII	GTNAC	2 cut(s)	874, 947
MalI	GATC	7 cut(s)	6, 183, 225, 471, 718, 726, 805
MboI	GATC	7 cut(s)	4, 181, 223, 469, 716, 724, 803
MboII	GAAGA	4 cut(s)	39, 376, 798, 819
MflI	RGATCY	3 cut(s)	223, 469, 803
MluCI	AATT	2 cut(s)	270, 534
MlyI	GAGTC	2 cut(s)	180, 755
MmeI	TCCRAC	4 cut(s)	42, 111, 789, 960
MnlI	CCTC	7 cut(s)	69, 168, 171, 373, 654, 852, 943
Mph1103I	ATGCAT	1 cut(s)	676
MroI	TCCGGA	1 cut(s)	226
MseI	TTAA	4 cut(s)	290, 395, 792, 837
MspA1I	CMGCKG	2 cut(s)	32, 667
MspCI	CTTAAG	1 cut(s)	791
MspI	CCGG	2 cut(s)	227, 528
MspR9I	CCNGG	3 cut(s)	100, 709, 801
Mva1269I	GAATGC	1 cut(s)	901
MvaI	CCWGG	3 cut(s)	100, 709, 801
MvnI	CGCG	1 cut(s)	165
MwoI	GCNNNNNNNGC	2 cut(s)	38, 664
NcoI	CCATGG	1 cut(s)	455
NdeII	GATC	7 cut(s)	4, 181, 223, 469, 716, 724, 803
NlaIII	CATG	3 cut(s)	459, 469, 544
NlaIV	GGNNCC	4 cut(s)	42, 129, 471, 489
NmeAIII	GCCGAG	1 cut(s)	343
NmuCI	GTSAC	1 cut(s)	947
NsiI	ATGCAT	1 cut(s)	676
PaqCI	CACCTGC	1 cut(s)	643
PcsI	WCGNNNNNNNCGW	1 cut(s)	128
PctI	GAATGC	1 cut(s)	901
PfeI	GAWTC	4 cut(s)	95, 341, 704, 821
PflMI	CCANNNNNTGG	1 cut(s)	461
PfoI	TCCNGGA	1 cut(s)	707
PkrI	GCNGC	2 cut(s)	34, 592
PleI	GAGTC	2 cut(s)	180, 755
PpsI	GAGTC	2 cut(s)	180, 755
PpuMI	RGGWCCY	1 cut(s)	127
Psp1406I	AACGTT	1 cut(s)	420
Psp5II	RGGWCCY	1 cut(s)	127
Psp6I	CCWGG	3 cut(s)	98, 707, 799
PspGI	CCWGG	3 cut(s)	98, 707, 799
PspN4I	GGNNCC	4 cut(s)	42, 129, 471, 489
PspPI	GGNCC	3 cut(s)	127, 234, 530
PspPPI	RGGWCCY	1 cut(s)	127
PstI	CTGCAG	1 cut(s)	835
PstNI	CAGNNNCTG	2 cut(s)	439, 635
PsuI	RGATCY	3 cut(s)	223, 469, 803
PvuII	CAGCTG	1 cut(s)	32
RsaI	GTAC	1 cut(s)	651
RsaNI	GTAC	1 cut(s)	650
SaqAI	TTAA	4 cut(s)	290, 395, 792, 837
SatI	GCNGC	2 cut(s)	33, 591
Sau3AI	GATC	7 cut(s)	4, 181, 223, 469, 716, 724, 803
Sau96I	GGNCC	3 cut(s)	127, 234, 530
SchI	GAGTC	2 cut(s)	180, 755
ScrFI	CCNGG	3 cut(s)	100, 709, 801
SfaNI	GCATC	4 cut(s)	398, 683, 786, 875
SfcI	CTRYAG	1 cut(s)	831
SinI	GGWCC	1 cut(s)	127
SmlI	CTYRAG	2 cut(s)	791, 912
SmoI	CTYRAG	2 cut(s)	791, 912
Sse9I	AATT	2 cut(s)	270, 534
SsiI	CCGC	3 cut(s)	69, 163, 665
StyD4I	CCNGG	3 cut(s)	98, 707, 799
StyI	CCWWGG	1 cut(s)	455
TaiI	ACGT	2 cut(s)	423, 954
TaqI	TCGA	1 cut(s)	184
TasI	AATT	2 cut(s)	270, 534
TatI	WGTACW	1 cut(s)	649
TfiI	GAWTC	4 cut(s)	95, 341, 704, 821
Tru1I	TTAA	4 cut(s)	290, 395, 792, 837
Tru9I	TTAA	4 cut(s)	290, 395, 792, 837
TscAI	CASTG	2 cut(s)	301, 748
TseFI	GTSAC	1 cut(s)	947
TseI	GCWGC	2 cut(s)	32, 590
Tsp45I	GTSAC	1 cut(s)	947
TspDTI	ATGAA	2 cut(s)	354, 813
TspRI	CASTG	2 cut(s)	301, 748
Van91I	CCANNNNNTGG	1 cut(s)	461
Vha464I	CTTAAG	1 cut(s)	791
VpaK11BI	GGWCC	1 cut(s)	127
ZraI	GACGTC	1 cut(s)	952
Zsp2I	ATGCAT	1 cut(s)	676

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.