MD02G1232800.v1.1

Belongs to the TBCA family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003164
Physical Location & Seq
Reverse (-)
27899841 .. 27902485
2645 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1232800.v1.1.491

Sequence Viewer

Length: 399 bp
ATGGGTTTCGTACTTTGCATTTATGAAGAAAATGATTCTTTTGATAGGACACCAATGGCAACCCTAAGAAATCTGAAAGTCAAGACTGGTACTTGTAAACGCCTAGTTAAGGAGCTTCATTCCTATGAGAAAGAGGTTGAGAGAGAAGCTGCCAAAACAGCAGACATGAAGGAGAAAGGAGCTGATCCGTATGACCTGAAGCAGCAGGAAAATGTGCTAGCTGAATCAAGGATGATGATTCCAGACTGTCGGAAACGCTTGGAGGCCTCATTAGCTGACCTGAAAGGAATTTTGGCTGAAGTAGAAGAAGAGTTGAACCAGAAGGAAGGCCCTGAAATTGAAGAAGCTCGAACTGTTATTGGAGAAGTTGACGCACTGTTCCAGACAACAGAAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TBCA

Protein Analysis

133

Amino Acids

15.01

Weight (kDa)

4.78

Isoelectric Point (pI)

34.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TBCA PF02970 21 - 120 1.7e-30 Tubulin binding cofactor A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 179
AcsI RAATTY 1 cut(s) 288
AcuI CTGAAG 2 cut(s) 218, 318
AfaI GTAC 2 cut(s) 12, 91
AfiI CCNNNNNNNGG 1 cut(s) 109
AgsI TTSAA 2 cut(s) 316, 341
AjuI GAANNNNNNNTTGG 2 cut(s) 275, 307
AluBI AGCT 6 cut(s) 115, 149, 182, 221, 275, 347
AluI AGCT 6 cut(s) 115, 149, 182, 221, 275, 347
AlwI GGATC 1 cut(s) 179
AoxI GGCC 2 cut(s) 264, 328
ApeKI GCWGC 2 cut(s) 149, 202
ApoI RAATTY 1 cut(s) 288
AspS9I GGNCC 1 cut(s) 329
AsuNHI GCTAGC 1 cut(s) 217
BbvI GCAGC 2 cut(s) 136, 214
BfaI CTAG 2 cut(s) 104, 218
BisI GCNGC 2 cut(s) 150, 203
BlsI GCNGC 2 cut(s) 151, 204
BmgT120I GGNCC 1 cut(s) 329
BmtI GCTAGC 1 cut(s) 221
Bsc4I CCNNNNNNNGG 1 cut(s) 109
Bse1I ACTGG 1 cut(s) 91
BseGI GGATG 1 cut(s) 237
BseLI CCNNNNNNNGG 1 cut(s) 109
BseNI ACTGG 1 cut(s) 91
BseXI GCAGC 2 cut(s) 136, 214
BshFI GGCC 2 cut(s) 266, 330
BslI CCNNNNNNNGG 1 cut(s) 109
BsnI GGCC 2 cut(s) 266, 330
Bsp143I GATC 1 cut(s) 184
BspANI GGCC 2 cut(s) 266, 330
BspOI GCTAGC 1 cut(s) 221
BspPI GGATC 1 cut(s) 179
BsrI ACTGG 1 cut(s) 91
BssMI GATC 1 cut(s) 184
Bst4CI ACNGT 3 cut(s) 248, 355, 378
Bst6I CTCTTC 1 cut(s) 303
BstC8I GCNNGC 1 cut(s) 219
BstDEI CTNAG 1 cut(s) 65
BstENI CCTNNNNNAGG 1 cut(s) 107
BstF5I GGATG 1 cut(s) 237
BstKTI GATC 1 cut(s) 187
BstMBI GATC 1 cut(s) 184
BstMWI GCNNNNNNNGC 2 cut(s) 158, 272
BstV1I GCAGC 2 cut(s) 136, 214
BsuRI GGCC 2 cut(s) 266, 330
BtsCI GGATG 1 cut(s) 237
BtsIMutI CAGTG 1 cut(s) 374
Cac8I GCNNGC 1 cut(s) 219
Cfr13I GGNCC 1 cut(s) 329
CseI GACGC 1 cut(s) 380
Csp6I GTAC 2 cut(s) 11, 90
CviAII CATG 1 cut(s) 166
CviQI GTAC 2 cut(s) 11, 90
DdeI CTNAG 1 cut(s) 65
DpnI GATC 1 cut(s) 186
DpnII GATC 1 cut(s) 184
Eam1104I CTCTTC 1 cut(s) 303
EarI CTCTTC 1 cut(s) 303
Eco147I AGGCCT 1 cut(s) 266
Eco57I CTGAAG 2 cut(s) 218, 318
EcoNI CCTNNNNNAGG 1 cut(s) 107
EcoO109I RGGNCCY 1 cut(s) 329
FaeI CATG 1 cut(s) 169
FaiI YATR 4 cut(s) 24, 126, 167, 192
FatI CATG 1 cut(s) 165
Fnu4HI GCNGC 2 cut(s) 150, 203
FokI GGATG 1 cut(s) 244
Fsp4HI GCNGC 2 cut(s) 150, 203
FspBI CTAG 2 cut(s) 104, 218
GluI GCNGC 2 cut(s) 150, 203
HaeIII GGCC 2 cut(s) 266, 330
HgaI GACGC 1 cut(s) 380
Hin1II CATG 1 cut(s) 169
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HinfI GANTC 3 cut(s) 35, 224, 238
Hpy166II GTNNAC 2 cut(s) 98, 370
Hpy188I TCNGA 2 cut(s) 75, 252
Hpy188III TCNNGA 3 cut(s) 82, 242, 382
Hpy8I GTNNAC 2 cut(s) 98, 370
HpyAV CCTTC 3 cut(s) 163, 316, 320
HpyCH4III ACNGT 3 cut(s) 248, 355, 378
HpyCH4V TGCA 1 cut(s) 18
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 272
HpyF3I CTNAG 1 cut(s) 65
Hsp92II CATG 1 cut(s) 169
Kzo9I GATC 1 cut(s) 184
LmnI GCTCC 2 cut(s) 112, 179
LpnPI CCDG 8 cut(s) 72, 191, 209, 255, 293, 332, 345, 395
Lsp1109I GCAGC 2 cut(s) 136, 214
MaeI CTAG 2 cut(s) 104, 218
MalI GATC 1 cut(s) 186
MboI GATC 1 cut(s) 184
MboII GAAGA 4 cut(s) 38, 317, 320, 353
MluCI AATT 2 cut(s) 288, 336
MmeI TCCRAC 1 cut(s) 230
MnlI CCTC 3 cut(s) 127, 256, 277
MseI TTAA 1 cut(s) 108
MslI CAYNNNNRTG 1 cut(s) 123
MwoI GCNNNNNNNGC 2 cut(s) 158, 272
NdeII GATC 1 cut(s) 184
NheI GCTAGC 1 cut(s) 217
NlaIII CATG 1 cut(s) 169
PceI AGGCCT 1 cut(s) 266
PfeI GAWTC 3 cut(s) 35, 224, 238
PkrI GCNGC 2 cut(s) 151, 204
PspPI GGNCC 1 cut(s) 329
RsaI GTAC 2 cut(s) 12, 91
RsaNI GTAC 2 cut(s) 11, 90
RseI CAYNNNNRTG 1 cut(s) 123
SaqAI TTAA 1 cut(s) 108
SatI GCNGC 2 cut(s) 150, 203
Sau3AI GATC 1 cut(s) 184
Sau96I GGNCC 1 cut(s) 329
SetI ASST 9 cut(s) 117, 138, 151, 184, 198, 223, 277, 282, 349
SmiMI CAYNNNNRTG 1 cut(s) 123
Sse9I AATT 2 cut(s) 288, 336
SseBI AGGCCT 1 cut(s) 266
SspMI CTAG 2 cut(s) 104, 218
StuI AGGCCT 1 cut(s) 266
TaaI ACNGT 3 cut(s) 248, 355, 378
TaqI TCGA 1 cut(s) 349
TasI AATT 2 cut(s) 288, 336
TfiI GAWTC 3 cut(s) 35, 224, 238
Tru1I TTAA 1 cut(s) 108
Tru9I TTAA 1 cut(s) 108
TscAI CASTG 1 cut(s) 381
TseI GCWGC 2 cut(s) 149, 202
TspDTI ATGAA 3 cut(s) 39, 107, 182
TspGWI ACGGA 1 cut(s) 177
TspRI CASTG 1 cut(s) 381
XagI CCTNNNNNAGG 1 cut(s) 107
XapI RAATTY 1 cut(s) 288
XspI CTAG 2 cut(s) 104, 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.