Rroxscaffold_4G00312330

Belongs to the TBCA family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003164
Physical Location & Seq
Reverse (-)
35237123 .. 35239902
2780 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00312330.1

Sequence Viewer

Length: 384 bp
ATGGCAACTGTAAGGAATCTGAAAATCAAGACTGGGACTTGTAAACGCCTCGTTAAGGAGTTTCATTCTTATGAGAAAGAGGTTGAGAGAGAGGCTGCGAAAACAGCAGACATGAAGGGGAAAGGAGCTGATCCTTATGACCTCAAGCAACAGAGCATTGATCTTTTGAAACAAACAAACATGAAAGGTACCGGAAATGTGCTGGCTGAATCAAGGATGATGAAACCCGATTGTCGAAAGCGCCTGGAGGCCTCACTAGCTGAGTTAAAAGGAACTTTGGCTGGGTTGGAAGAAGAGTTGAACCAGAAGGAAGGCCCTGAAATTGAAGAAGCTCGGACCATTATTGCAGAAGTTGATAAGTTGTTCCAGACAACAGAAACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TBCA

Protein Analysis

127

Amino Acids

14.29

Weight (kDa)

6.63

Isoelectric Point (pI)

26.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TBCA PF02970 3 - 115 1.3e-23 Tubulin binding cofactor A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 188
AccB1I GGYRCC 1 cut(s) 188
AclWI GGATC 1 cut(s) 125
AfaI GTAC 1 cut(s) 190
AfiI CCNNNNNNNGG 1 cut(s) 55
AgsI TTSAA 3 cut(s) 169, 301, 326
AjnI CCWGG 1 cut(s) 243
AluBI AGCT 3 cut(s) 128, 260, 332
AluI AGCT 3 cut(s) 128, 260, 332
AlwI GGATC 1 cut(s) 125
AoxI GGCC 2 cut(s) 249, 313
ApeKI GCWGC 1 cut(s) 95
Asp718I GGTACC 1 cut(s) 188
AspLEI GCGC 1 cut(s) 243
AspS9I GGNCC 2 cut(s) 314, 336
AvaII GGWCC 1 cut(s) 336
BanI GGYRCC 1 cut(s) 188
BbvI GCAGC 1 cut(s) 82
BciT130I CCWGG 1 cut(s) 245
BfaI CTAG 2 cut(s) 257, 382
BfoI RGCGCY 1 cut(s) 244
BisI GCNGC 1 cut(s) 96
BlsI GCNGC 1 cut(s) 97
Bme1390I CCNGG 1 cut(s) 245
Bme18I GGWCC 1 cut(s) 336
BmgT120I GGNCC 2 cut(s) 314, 336
BmiI GGNNCC 1 cut(s) 190
BmrFI CCNGG 1 cut(s) 245
BmrI ACTGGG 1 cut(s) 42
BmuI ACTGGG 1 cut(s) 42
BpmI CTGGAG 1 cut(s) 266
BpuEI CTTGAG 1 cut(s) 128
BsaWI WCCGGW 1 cut(s) 191
Bsc4I CCNNNNNNNGG 1 cut(s) 55
Bse1I ACTGG 1 cut(s) 37
BseBI CCWGG 1 cut(s) 245
BseGI GGATG 1 cut(s) 222
BseLI CCNNNNNNNGG 1 cut(s) 55
BseMII CTCAG 1 cut(s) 252
BseNI ACTGG 1 cut(s) 37
BseXI GCAGC 1 cut(s) 82
BseYI CCCAGC 1 cut(s) 281
BshFI GGCC 2 cut(s) 251, 315
BshNI GGYRCC 1 cut(s) 188
BsiSI CCGG 1 cut(s) 192
BslFI GGGAC 1 cut(s) 49
BslI CCNNNNNNNGG 1 cut(s) 55
BsmFI GGGAC 1 cut(s) 49
BsnI GGCC 2 cut(s) 251, 315
Bsp143I GATC 2 cut(s) 130, 160
BspANI GGCC 2 cut(s) 251, 315
BspCNI CTCAG 1 cut(s) 253
BspLI GGNNCC 1 cut(s) 190
BspPI GGATC 1 cut(s) 125
BspT107I GGYRCC 1 cut(s) 188
BsrI ACTGG 1 cut(s) 37
BssMI GATC 2 cut(s) 130, 160
Bst2UI CCWGG 1 cut(s) 245
Bst4CI ACNGT 1 cut(s) 10
Bst6I CTCTTC 1 cut(s) 288
BstC8I GCNNGC 1 cut(s) 204
BstDEI CTNAG 1 cut(s) 261
BstENI CCTNNNNNAGG 1 cut(s) 53
BstF5I GGATG 1 cut(s) 222
BstH2I RGCGCY 1 cut(s) 244
BstHHI GCGC 1 cut(s) 243
BstKTI GATC 2 cut(s) 133, 163
BstMBI GATC 2 cut(s) 130, 160
BstMWI GCNNNNNNNGC 2 cut(s) 104, 257
BstNI CCWGG 1 cut(s) 245
BstSCI CCNGG 1 cut(s) 243
BstV1I GCAGC 1 cut(s) 82
BsuRI GGCC 2 cut(s) 251, 315
BtsCI GGATG 1 cut(s) 222
Cac8I GCNNGC 1 cut(s) 204
CfoI GCGC 1 cut(s) 243
Cfr13I GGNCC 2 cut(s) 314, 336
Csp6I GTAC 1 cut(s) 189
CviAII CATG 2 cut(s) 112, 181
CviJI RGCY 8 cut(s) 95, 128, 206, 251, 260, 281, 315, 332
CviKI_1 RGCY 8 cut(s) 95, 128, 206, 251, 260, 281, 315, 332
CviQI GTAC 1 cut(s) 189
DdeI CTNAG 1 cut(s) 261
DpnI GATC 2 cut(s) 132, 162
DpnII GATC 2 cut(s) 130, 160
Eam1104I CTCTTC 1 cut(s) 288
EarI CTCTTC 1 cut(s) 288
Eco147I AGGCCT 1 cut(s) 251
Eco47I GGWCC 1 cut(s) 336
EcoNI CCTNNNNNAGG 1 cut(s) 53
EcoO109I RGGNCCY 1 cut(s) 314
EcoRII CCWGG 1 cut(s) 243
FaeI CATG 2 cut(s) 115, 184
FaiI YATR 4 cut(s) 72, 113, 138, 182
FaqI GGGAC 1 cut(s) 49
FatI CATG 2 cut(s) 111, 180
Fnu4HI GCNGC 1 cut(s) 96
FokI GGATG 1 cut(s) 229
Fsp4HI GCNGC 1 cut(s) 96
FspBI CTAG 2 cut(s) 257, 382
GlaI GCGC 1 cut(s) 242
GluI GCNGC 1 cut(s) 96
GsaI CCCAGC 1 cut(s) 285
GsuI CTGGAG 1 cut(s) 266
HaeII RGCGCY 1 cut(s) 244
HaeIII GGCC 2 cut(s) 251, 315
HapII CCGG 1 cut(s) 192
HhaI GCGC 1 cut(s) 243
Hin1II CATG 2 cut(s) 115, 184
Hin6I GCGC 1 cut(s) 241
HinP1I GCGC 1 cut(s) 241
HinfI GANTC 2 cut(s) 16, 209
HpaII CCGG 1 cut(s) 192
Hpy166II GTNNAC 1 cut(s) 44
Hpy188I TCNGA 2 cut(s) 21, 336
Hpy188III TCNNGA 2 cut(s) 28, 367
Hpy8I GTNNAC 1 cut(s) 44
HpyAV CCTTC 3 cut(s) 109, 301, 305
HpyCH4III ACNGT 1 cut(s) 10
HpyCH4V TGCA 1 cut(s) 347
HpyF10VI GCNNNNNNNGC 2 cut(s) 104, 257
HpyF3I CTNAG 1 cut(s) 261
Hsp92II CATG 2 cut(s) 115, 184
HspAI GCGC 1 cut(s) 241
KpnI GGTACC 1 cut(s) 192
Kzo9I GATC 2 cut(s) 130, 160
LmnI GCTCC 1 cut(s) 125
LpnPI CCDG 9 cut(s) 18, 188, 205, 230, 257, 267, 317, 330, 380
Lsp1109I GCAGC 1 cut(s) 82
MaeI CTAG 2 cut(s) 257, 382
MalI GATC 2 cut(s) 132, 162
MboI GATC 2 cut(s) 130, 160
MboII GAAGA 3 cut(s) 302, 305, 338
MluCI AATT 1 cut(s) 321
MmeI TCCRAC 1 cut(s) 267
MnlI CCTC 6 cut(s) 59, 73, 85, 152, 241, 262
MseI TTAA 2 cut(s) 54, 266
MslI CAYNNNNRTG 1 cut(s) 69
MspI CCGG 1 cut(s) 192
MspR9I CCNGG 1 cut(s) 245
MvaI CCWGG 1 cut(s) 245
MwoI GCNNNNNNNGC 2 cut(s) 104, 257
NdeII GATC 2 cut(s) 130, 160
NlaIII CATG 2 cut(s) 115, 184
NlaIV GGNNCC 1 cut(s) 190
PceI AGGCCT 1 cut(s) 251
PfeI GAWTC 2 cut(s) 16, 209
PkrI GCNGC 1 cut(s) 97
Psp6I CCWGG 1 cut(s) 243
PspFI CCCAGC 1 cut(s) 281
PspGI CCWGG 1 cut(s) 243
PspN4I GGNNCC 1 cut(s) 190
PspPI GGNCC 2 cut(s) 314, 336
RsaI GTAC 1 cut(s) 190
RsaNI GTAC 1 cut(s) 189
RseI CAYNNNNRTG 1 cut(s) 69
SaqAI TTAA 2 cut(s) 54, 266
SatI GCNGC 1 cut(s) 96
Sau3AI GATC 2 cut(s) 130, 160
Sau96I GGNCC 2 cut(s) 314, 336
ScrFI CCNGG 1 cut(s) 245
SetI ASST 7 cut(s) 84, 130, 144, 190, 262, 334, 383
SinI GGWCC 1 cut(s) 336
SmiMI CAYNNNNRTG 1 cut(s) 69
SmlI CTYRAG 1 cut(s) 143
SmoI CTYRAG 1 cut(s) 143
Sse9I AATT 1 cut(s) 321
SseBI AGGCCT 1 cut(s) 251
SspMI CTAG 2 cut(s) 257, 382
StuI AGGCCT 1 cut(s) 251
StyD4I CCNGG 1 cut(s) 243
TaaI ACNGT 1 cut(s) 10
TaqI TCGA 1 cut(s) 235
TasI AATT 1 cut(s) 321
TfiI GAWTC 2 cut(s) 16, 209
Tru1I TTAA 2 cut(s) 54, 266
Tru9I TTAA 2 cut(s) 54, 266
TseI GCWGC 1 cut(s) 95
TspDTI ATGAA 4 cut(s) 53, 128, 197, 236
VpaK11BI GGWCC 1 cut(s) 336
XagI CCTNNNNNAGG 1 cut(s) 53
XspI CTAG 2 cut(s) 257, 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.