MD07G1080900.v1.1

Belongs to the TBCA family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003164
Physical Location & Seq
Forward (+)
7848799 .. 7851495
2697 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1080900.v1.1.491

Sequence Viewer

Length: 369 bp
ATGTTTACCATTTATGGGGTTACCATGGCAACCCTAAGAAATCTGAAAATCAAGACTGGTACTTGCAAACGCATCGTTAAGGAGCTTCATTCTTATGAGAAAGAGGTTGAGAGAGAAGCTGCAAAAACAGCAGACATGAAAGAGAAAGGAGCTGATCCTTATGACCTGAAGCAACAGGAAAATGTGCTGGCTGAATCTAGGATGATGATTCCTGATTGCCGGAAACGCTTGGAGGCCTCATTAGCTGACCTGAAAGGAATTTTGGCTGAAGTAGAAGAAGAGTTGAACCAGAAGGAAGGCCCTGAAATTGAAGAAGCTCGAACCATTATTGGAGAAGTTGATACACTGTTTCAGACAACAGAAGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TBCA

Protein Analysis

123

Amino Acids

13.86

Weight (kDa)

4.97

Isoelectric Point (pI)

29.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TBCA PF02970 11 - 110 8.6e-31 Tubulin binding cofactor A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 149
AcsI RAATTY 1 cut(s) 258
AcuI CTGAAG 2 cut(s) 188, 288
AfaI GTAC 1 cut(s) 61
AfiI CCNNNNNNNGG 1 cut(s) 15
AgsI TTSAA 2 cut(s) 286, 311
AjuI GAANNNNNNNTTGG 2 cut(s) 245, 277
AluBI AGCT 5 cut(s) 85, 119, 152, 245, 317
AluI AGCT 5 cut(s) 85, 119, 152, 245, 317
AlwI GGATC 1 cut(s) 149
AoxI GGCC 2 cut(s) 234, 298
ApeKI GCWGC 1 cut(s) 119
ApoI RAATTY 1 cut(s) 258
AspS9I GGNCC 1 cut(s) 299
BbvI GCAGC 1 cut(s) 106
BcgI CGANNNNNNTGC 2 cut(s) 55, 89
BfaI CTAG 1 cut(s) 198
BisI GCNGC 1 cut(s) 120
BlsI GCNGC 1 cut(s) 121
BmgT120I GGNCC 1 cut(s) 299
BmsI GCATC 1 cut(s) 81
BsaJI CCNNGG 1 cut(s) 24
Bsc4I CCNNNNNNNGG 1 cut(s) 15
Bse1I ACTGG 1 cut(s) 61
BseDI CCNNGG 1 cut(s) 24
BseGI GGATG 1 cut(s) 207
BseLI CCNNNNNNNGG 1 cut(s) 15
BseNI ACTGG 1 cut(s) 61
BseXI GCAGC 1 cut(s) 106
BshFI GGCC 2 cut(s) 236, 300
BsiSI CCGG 1 cut(s) 220
BslI CCNNNNNNNGG 1 cut(s) 15
BsnI GGCC 2 cut(s) 236, 300
Bsp143I GATC 1 cut(s) 154
Bsp19I CCATGG 1 cut(s) 24
BspANI GGCC 2 cut(s) 236, 300
BspPI GGATC 1 cut(s) 149
BsrI ACTGG 1 cut(s) 61
BssECI CCNNGG 1 cut(s) 24
BssMI GATC 1 cut(s) 154
BssT1I CCWWGG 1 cut(s) 24
Bst4CI ACNGT 1 cut(s) 348
Bst6I CTCTTC 1 cut(s) 273
BstC8I GCNNGC 1 cut(s) 189
BstDEI CTNAG 1 cut(s) 35
BstDSI CCRYGG 1 cut(s) 24
BstEII GGTNACC 1 cut(s) 19
BstF5I GGATG 1 cut(s) 207
BstKTI GATC 1 cut(s) 157
BstMBI GATC 1 cut(s) 154
BstMWI GCNNNNNNNGC 3 cut(s) 128, 225, 242
BstPI GGTNACC 1 cut(s) 19
BstV1I GCAGC 1 cut(s) 106
BsuRI GGCC 2 cut(s) 236, 300
BtgI CCRYGG 1 cut(s) 24
BtsCI GGATG 1 cut(s) 207
BtsIMutI CAGTG 1 cut(s) 344
Cac8I GCNNGC 1 cut(s) 189
Cfr13I GGNCC 1 cut(s) 299
Csp6I GTAC 1 cut(s) 60
CviAII CATG 2 cut(s) 25, 136
CviJI RGCY 9 cut(s) 85, 119, 152, 191, 236, 245, 266, 300, 317
CviKI_1 RGCY 9 cut(s) 85, 119, 152, 191, 236, 245, 266, 300, 317
CviQI GTAC 1 cut(s) 60
DdeI CTNAG 1 cut(s) 35
DpnI GATC 1 cut(s) 156
DpnII GATC 1 cut(s) 154
Eam1104I CTCTTC 1 cut(s) 273
EarI CTCTTC 1 cut(s) 273
Eco130I CCWWGG 1 cut(s) 24
Eco147I AGGCCT 1 cut(s) 236
Eco57I CTGAAG 2 cut(s) 188, 288
Eco91I GGTNACC 1 cut(s) 19
EcoO109I RGGNCCY 1 cut(s) 299
EcoO65I GGTNACC 1 cut(s) 19
EcoT14I CCWWGG 1 cut(s) 24
ErhI CCWWGG 1 cut(s) 24
FaeI CATG 2 cut(s) 28, 139
FaiI YATR 5 cut(s) 15, 26, 96, 137, 162
FatI CATG 2 cut(s) 24, 135
Fnu4HI GCNGC 1 cut(s) 120
FokI GGATG 1 cut(s) 214
Fsp4HI GCNGC 1 cut(s) 120
FspBI CTAG 1 cut(s) 198
GluI GCNGC 1 cut(s) 120
HaeIII GGCC 2 cut(s) 236, 300
HapII CCGG 1 cut(s) 220
Hin1II CATG 2 cut(s) 28, 139
HinfI GANTC 2 cut(s) 194, 208
HpaII CCGG 1 cut(s) 220
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 2 cut(s) 45, 354
Hpy188III TCNNGA 2 cut(s) 52, 212
Hpy8I GTNNAC 1 cut(s) 6
HpyAV CCTTC 2 cut(s) 286, 290
HpyCH4III ACNGT 1 cut(s) 348
HpyCH4V TGCA 2 cut(s) 66, 122
HpyF10VI GCNNNNNNNGC 3 cut(s) 128, 225, 242
HpyF3I CTNAG 1 cut(s) 35
Hsp92II CATG 2 cut(s) 28, 139
Kzo9I GATC 1 cut(s) 154
LmnI GCTCC 2 cut(s) 82, 149
LpnPI CCDG 9 cut(s) 42, 161, 173, 179, 225, 233, 263, 302, 315
Lsp1109I GCAGC 1 cut(s) 106
LweI GCATC 1 cut(s) 81
MaeI CTAG 1 cut(s) 198
MaeIII GTNAC 1 cut(s) 19
MalI GATC 1 cut(s) 156
MboI GATC 1 cut(s) 154
MboII GAAGA 3 cut(s) 287, 290, 323
MluCI AATT 2 cut(s) 258, 306
MnlI CCTC 3 cut(s) 97, 226, 247
MseI TTAA 1 cut(s) 78
MslI CAYNNNNRTG 1 cut(s) 93
MspI CCGG 1 cut(s) 220
MwoI GCNNNNNNNGC 3 cut(s) 128, 225, 242
NcoI CCATGG 1 cut(s) 24
NdeII GATC 1 cut(s) 154
NlaIII CATG 2 cut(s) 28, 139
PceI AGGCCT 1 cut(s) 236
PfeI GAWTC 2 cut(s) 194, 208
PkrI GCNGC 1 cut(s) 121
PspEI GGTNACC 1 cut(s) 19
PspPI GGNCC 1 cut(s) 299
RsaI GTAC 1 cut(s) 61
RsaNI GTAC 1 cut(s) 60
RseI CAYNNNNRTG 1 cut(s) 93
SaqAI TTAA 1 cut(s) 78
SatI GCNGC 1 cut(s) 120
Sau3AI GATC 1 cut(s) 154
Sau96I GGNCC 1 cut(s) 299
SetI ASST 8 cut(s) 87, 108, 121, 154, 168, 247, 252, 319
SfaNI GCATC 1 cut(s) 81
SmiMI CAYNNNNRTG 1 cut(s) 93
Sse9I AATT 2 cut(s) 258, 306
SseBI AGGCCT 1 cut(s) 236
SspMI CTAG 1 cut(s) 198
StuI AGGCCT 1 cut(s) 236
StyI CCWWGG 1 cut(s) 24
TaaI ACNGT 1 cut(s) 348
TaqI TCGA 1 cut(s) 319
TasI AATT 2 cut(s) 258, 306
TfiI GAWTC 2 cut(s) 194, 208
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TscAI CASTG 1 cut(s) 351
TseI GCWGC 1 cut(s) 119
TspDTI ATGAA 2 cut(s) 77, 152
TspRI CASTG 1 cut(s) 351
XapI RAATTY 1 cut(s) 258
XspI CTAG 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.