Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0001996

Physical Location & Seq

Genomic Coordinates Reverse (-)

9475 .. 11407

Gene Structure 1933 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1000200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1659 bp

ATGGCTTCCGTGATGTATGCGTTGTTGCTGTGCCTTACAGCCGGGGCACTTTCAGTGGTCCAGGGCGAAGACCCTTACCTGTTCTTCACATGGAATGTCACCTACGGAACCATCTCTCCCCTCGGAGTCCCCCAGCAAGCAATTCTCATCAATGGCCAGTTCCCAGGACCCAACATCAACTCCACCTCCAACAACAACATCGTCTTGAACGTCTTCAACAACCTCGATGAGCCATTCCTTTTGACATGGAGCGGCATCCAGCAGAGGAAGAATTCGTGGCAGGATGGAACCCTTGGAACCATGTGCCCCATCGTCCCTGGCACCAACTTCACCTACCGCTTCCAAGTCAAGGATCAGATCGGGAGCTACATGTACTATCCCGTCACTGCCATGCACAGGGCAGCAGGCGGCTTTGGTGGACTCCGCATCAACAGTCGTCTGCTCATTCCCGTCCCCTATGCTGATCCCGAGGATGAATACACCGTTATGATCGGCGACTGGTACACCAAAAGTCACACCGCTCTCAAGAAATTATTGGACAGCGGCCGCACCATGGGAAGGCCCGACGGCATCCTAATCAACGGCAAGAACGCCAAGGGAGACGGCAAAGACGAGCCCCTCTTCACCATGAAGCCAGGGAAGACCTACAAGTACAGGGTCTGCAACGTGGGCTTCAAGAACTCCCTCAACTTCAGGATCCAAGGCCACCCCTTAAAGCTTGTCGAGATGGAAGGCTCCCACACCGTCCAGAACACCTACGAGTCCCTTGACGTGCACTTGGGGCAATGCTTCTCGTTGCTCGTCACTGCCGACAAAGAGCGCAAGGACTACTACATGGTAGCCTCGACCCGATTCACCAAGACCGTGCTCACCGGCAAAGGCATAATCCGCTATGAAAACAGTAACGGCCCTGCATCCCCCGAGCTCCCCGAAGCTCCTGTAGGATGGGCCTGGTCTCTCAACCAGTTCCGTTCCTTCCGCTGGAACCTTACTGCCAGCGCTGCCAGGCCTAATCCTCAGGGCTCGTACCACTACGGAAAGATCAACATCACCCGTACCATCAAGATCGTCAACTCCGCCTCGATGGTGAATGGCAAGCTCCGCTATGCCATCAACGGAGTCTCCTACGTGGAATCCGAAACCCCACTCAAGCTGGCTCAGTACTTTGGTGTGGCCGACAAGGTCTTCAAGTACGACACCATCCAAGATGAACCCCCAGCCACCGTGGAGGACAAGGTCACCTTGGCACCCAACGTTGTCAACCAGACCTTCCGTGACTTTGTAGAGATCATCTTTGAGAACCACGAGAAGAGCATTCAGTCATGGCATTTGGATGGATACTCCTTCTTTGCCGTCGCCATTGAGCCCGGGAGGTGGACCCCAGAAAAGAGAAAGAACTACAACCTTCTGGACGGAGTGAGCAGGCACACCATCCAAGTGTTCCCCAAATCCTGGGCGGCCATAATGTTGACCTTCGACAATGCCGGAATGTGGAACGTGAGGTCGGAACAACCGGAGAGGCGCTACCTCGGACAACAGTTCTACGTGAGCGTCCTGTCACCTGCACGCTCCCTCAGGGACGAGTACAACCTCCCAGAAACCACCCAACTCTGTGGCATTGTCAAGGATTTGCCTAAGCCCCCTCCATACAGCGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00053 ko01100 map00053 map01100

Pfam Domains

Protein Families

Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

553

Amino Acids

62.12

Weight (kDa)

8.86

Isoelectric Point (pI)

36.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cu-oxidase_3	PF07732	32 - 146	9.3e-40	Multicopper oxidase
Cu-oxidase	PF00394	158 - 300	1.2e-41	Multicopper oxidase
Cu-oxidase_2	PF07731	381 - 521	1.5e-27	Multicopper oxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001996)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55560 AT1G55570 AT3G13390 AT3G13400 AT3G13400
fragaria_vesca	FvH4_3g46080 FvH4_6g20700
malus_domestica	MD03G1000200.v1.1 MD03G1000500.v1.1 MD11G1000300.v1.1 MD11G1000400.v1.1
prunus_persica	Prupe.6G000400_v2.0.a1 Prupe.6G000500_v2.0.a1
pyrus_communis	pycom03g00050 pycom11g00060
rosa_chinensis	RchiOBHm_Chr3g0476601 RchiOBHm_Chr3g0476621 RchiOBHm_Chr5g0084141
rosa_laevigata	RLG00000001981 RLG00000001982 RLG00000023759 RLG00000037086
rosa_multiflora	Rmu_co7980006.1_g000001 Rmu_sc0008241.1_g000008 Rmu_sc0008241.1_g000011 Rmu_sc0014688.1_g000001 Rmu_sc0035447.1_g000001
rosa_roxburghii	Rroxscaffold_1G00000500 Rroxscaffold_6G00405150 Rroxscaffold_6G00405170
rosa_rugosa	Rorug03G0155600 Rorug03G0155900 Rorug05G0489500
rosa_samantha	Rh3AG206200 Rh3CG232600 Rh3DG232400 Rh3DG232600 Rh5AG542100 Rh5CG589700 Rh5DG576100 Rh7DG309900
rosa_wichuraiana	Rw3G018780 Rw3G018790 Rw5G050430 Rw7G026320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1570
Acc36I	ACCTGC	1 cut(s)	1570
AccB1I	GGYRCC	2 cut(s)	320, 1246
AccB7I	CCANNNNNTGG	1 cut(s)	1452
AccBSI	CCGCTC	2 cut(s)	252, 521
AclI	AACGTT	1 cut(s)	1254
AclWI	GGATC	4 cut(s)	360, 458, 691, 704
AcoI	YGGCCR	4 cut(s)	154, 544, 1173, 1458
AcsI	RAATTY	1 cut(s)	271
AcuI	CTGAAG	1 cut(s)	676
AfaI	GTAC	8 cut(s)	374, 503, 653, 1028, 1057, 1163, 1193, 1586
AfeI	AGCGCT	2 cut(s)	1000, 1654
AfiI	CCNNNNNNNGG	8 cut(s)	349, 396, 553, 558, 981, 1374, 1452, 1491
AflIII	ACRYGT	1 cut(s)	369
AgsI	TTSAA	4 cut(s)	208, 217, 676, 1189
AjiI	CACGTC	1 cut(s)	772
AjnI	CCWGG	7 cut(s)	60, 163, 316, 634, 950, 1004, 1451
AloI	GAACNNNNNNTCC	2 cut(s)	100, 132
AluBI	AGCT	6 cut(s)	366, 718, 925, 935, 1099, 1153
AluI	AGCT	6 cut(s)	366, 718, 925, 935, 1099, 1153
Alw21I	GWGCWC	3 cut(s)	777, 870, 927
Alw26I	GTCTC	3 cut(s)	594, 960, 1126
Alw44I	GTGCAC	1 cut(s)	773
AlwI	GGATC	4 cut(s)	360, 458, 691, 704
AlwNI	CAGNNNCTG	1 cut(s)	660
Ama87I	CYCGRG	3 cut(s)	467, 920, 1367
Aor51HI	AGCGCT	2 cut(s)	1000, 1654
AoxI	GGCC	9 cut(s)	154, 544, 560, 703, 907, 948, 1007, 1173, 1458
ApaLI	GTGCAC	1 cut(s)	773
ApeKI	GCWGC	2 cut(s)	401, 1001
ApoI	RAATTY	1 cut(s)	271
Asp700I	GAANNNNTTC	1 cut(s)	212
AspLEI	GCGC	4 cut(s)	822, 1001, 1524, 1655
AspS9I	GGNCC	6 cut(s)	58, 167, 561, 908, 948, 1377
AsuC2I	CCSGG	3 cut(s)	43, 1368, 1369
AsuHPI	GGTGA	9 cut(s)	91, 322, 616, 847, 862, 1042, 1099, 1231, 1551
AvaI	CYCGRG	3 cut(s)	467, 920, 1367
AvaII	GGWCC	3 cut(s)	58, 167, 1377
AxyI	CCTNAGG	2 cut(s)	1017, 1574
BaeGI	GKGCMC	3 cut(s)	49, 308, 777
BalI	TGGCCA	1 cut(s)	156
BamHI	GGATCC	1 cut(s)	696
BanI	GGYRCC	2 cut(s)	320, 1246
BanII	GRGCYC	4 cut(s)	618, 927, 1025, 1368
BauI	CACGAG	1 cut(s)	1304
BbsI	GAAGAC	4 cut(s)	75, 205, 647, 1177
Bbv12I	GWGCWC	3 cut(s)	777, 870, 927
BbvI	GCAGC	2 cut(s)	413, 988
BceAI	ACGGC	5 cut(s)	583, 598, 619, 922, 1337
BciT130I	CCWGG	7 cut(s)	62, 165, 318, 636, 952, 1006, 1453
BciVI	GTATCC	1 cut(s)	1331
BcnI	CCSGG	3 cut(s)	43, 1368, 1369
BcoDI	GTCTC	3 cut(s)	594, 960, 1126
BfmI	CTRYAG	1 cut(s)	939
BfoI	RGCGCY	3 cut(s)	1002, 1525, 1656
BfuAI	ACCTGC	1 cut(s)	1570
BfuI	GTATCC	1 cut(s)	1331
BisI	GCNGC	7 cut(s)	253, 402, 409, 544, 547, 1002, 1458
BlsI	GCNGC	7 cut(s)	254, 403, 410, 545, 548, 1003, 1459
BmcAI	AGTACT	1 cut(s)	1163
Bme18I	GGWCC	3 cut(s)	58, 167, 1377
BmeT110I	CYCGRG	3 cut(s)	467, 920, 1367
BmgBI	CACGTC	1 cut(s)	772
BmgT120I	GGNCC	6 cut(s)	58, 167, 561, 908, 948, 1377
BmsI	GCATC	4 cut(s)	264, 435, 579, 923
BpiI	GAAGAC	4 cut(s)	75, 205, 647, 1177
Bpu10I	CCTNAGC	1 cut(s)	1635
BpuEI	CTTGAG	2 cut(s)	509, 1133
BpuMI	CCSGG	3 cut(s)	43, 1368, 1369
BsaAI	YACGTR	2 cut(s)	1129, 1546
BsaBI	GATNNNNATC	1 cut(s)	1046
BsaI	GGTCTC	1 cut(s)	960
BsaWI	WCCGGW	1 cut(s)	1513
BsaXI	ACNNNNNCTCC	8 cut(s)	100, 130, 164, 170, 194, 200, 1106, 1136
Bsc4I	CCNNNNNNNGG	8 cut(s)	349, 396, 553, 558, 981, 1374, 1452, 1491
Bse118I	RCCGGY	1 cut(s)	872
Bse1I	ACTGG	3 cut(s)	157, 503, 964
Bse21I	CCTNAGG	2 cut(s)	1017, 1574
Bse3DI	GCAATG	1 cut(s)	791
Bse8I	GATNNNNATC	1 cut(s)	1046
BseBI	CCWGG	7 cut(s)	62, 165, 318, 636, 952, 1006, 1453
BseGI	GGATG	9 cut(s)	255, 289, 478, 570, 914, 950, 1200, 1339, 1431
BseJI	GATNNNNATC	1 cut(s)	1046
BseLI	CCNNNNNNNGG	8 cut(s)	349, 396, 553, 558, 981, 1374, 1452, 1491
BseMI	GCAATG	1 cut(s)	791
BseMII	CTCAG	3 cut(s)	1031, 1172, 1588
BseNI	ACTGG	3 cut(s)	157, 503, 964
BseSI	GKGCMC	3 cut(s)	49, 308, 777
BseX3I	CGGCCG	1 cut(s)	544
BseXI	GCAGC	2 cut(s)	413, 988
BseYI	CCCAGC	2 cut(s)	132, 1216
BsgI	GTGCAG	1 cut(s)	1548
Bsh1285I	CGRYCG	1 cut(s)	547
BshFI	GGCC	9 cut(s)	156, 546, 562, 705, 909, 950, 1009, 1175, 1460
BshNI	GGYRCC	2 cut(s)	320, 1246
BsiEI	CGRYCG	1 cut(s)	547
BsiHKAI	GWGCWC	3 cut(s)	777, 870, 927
BsiHKCI	CYCGRG	3 cut(s)	467, 920, 1367
BsiSI	CCGG	5 cut(s)	42, 873, 1368, 1485, 1514
BslFI	GGGAC	5 cut(s)	113, 299, 437, 748, 1592
BslI	CCNNNNNNNGG	8 cut(s)	349, 396, 553, 558, 981, 1374, 1452, 1491
BsmAI	GTCTC	3 cut(s)	594, 960, 1126
BsmBI	CGTCTC	1 cut(s)	594
BsmFI	GGGAC	5 cut(s)	113, 299, 437, 748, 1592
BsmI	GAATGC	1 cut(s)	1314
BsnI	GGCC	9 cut(s)	156, 546, 562, 705, 909, 950, 1009, 1175, 1460
Bso31I	GGTCTC	1 cut(s)	960
BsoBI	CYCGRG	3 cut(s)	467, 920, 1367
Bsp1286I	GDGCHC	8 cut(s)	49, 308, 618, 777, 870, 927, 1025, 1368
Bsp143I	GATC	8 cut(s)	352, 357, 463, 489, 696, 1041, 1065, 1287
Bsp19I	CCATGG	1 cut(s)	552
BspANI	GGCC	9 cut(s)	156, 546, 562, 705, 909, 950, 1009, 1175, 1460
BspCNI	CTCAG	3 cut(s)	1030, 1171, 1587
BspMI	ACCTGC	1 cut(s)	1570
BspPI	GGATC	4 cut(s)	360, 458, 691, 704
BspQI	GCTCTTC	1 cut(s)	1304
BspT107I	GGYRCC	2 cut(s)	320, 1246
BspTNI	GGTCTC	1 cut(s)	960
BsrBI	CCGCTC	2 cut(s)	252, 521
BsrDI	GCAATG	1 cut(s)	791
BsrFI	RCCGGY	1 cut(s)	872
BsrI	ACTGG	3 cut(s)	157, 503, 964
BssAI	RCCGGY	1 cut(s)	872
BssMI	GATC	8 cut(s)	352, 357, 463, 489, 696, 1041, 1065, 1287
BssSI	CACGAG	1 cut(s)	1304
BssT1I	CCWWGG	5 cut(s)	292, 552, 594, 700, 1242
Bst2BI	CACGAG	1 cut(s)	1304
Bst2UI	CCWGG	7 cut(s)	62, 165, 318, 636, 952, 1006, 1453
Bst4CI	ACNGT	7 cut(s)	434, 484, 745, 865, 902, 1225, 1539
Bst6I	CTCTTC	2 cut(s)	626, 1304
BstBAI	YACGTR	2 cut(s)	1129, 1546
BstC8I	GCNNGC	7 cut(s)	138, 406, 997, 1097, 1155, 1424, 1567
BstDEI	CTNAG	4 cut(s)	1017, 1158, 1574, 1635
BstDSI	CCRYGG	2 cut(s)	552, 1224
BstEII	GGTNACC	1 cut(s)	1237
BstF5I	GGATG	9 cut(s)	255, 289, 478, 570, 914, 950, 1200, 1339, 1431
BstH2I	RGCGCY	3 cut(s)	1002, 1525, 1656
BstHHI	GCGC	4 cut(s)	822, 1001, 1524, 1655
BstKTI	GATC	8 cut(s)	355, 360, 466, 492, 699, 1044, 1068, 1290
BstMAI	GTCTC	3 cut(s)	594, 960, 1126
BstMBI	GATC	8 cut(s)	352, 357, 463, 489, 696, 1041, 1065, 1287
BstMCI	CGRYCG	1 cut(s)	547
BstMWI	GCNNNNNNNGC	5 cut(s)	669, 781, 888, 1001, 1101
BstNI	CCWGG	7 cut(s)	62, 165, 318, 636, 952, 1006, 1453
BstNSI	RCATGY	1 cut(s)	373
BstPI	GGTNACC	1 cut(s)	1237
BstSFI	CTRYAG	1 cut(s)	939
BstSLI	GKGCMC	3 cut(s)	49, 308, 777
BstV1I	GCAGC	2 cut(s)	413, 988
BstV2I	GAAGAC	4 cut(s)	75, 205, 647, 1177
BstX2I	RGATCY	1 cut(s)	696
BstXI	CCANNNNNNTGG	1 cut(s)	1613
BstYI	RGATCY	1 cut(s)	696
BstZI	CGGCCG	1 cut(s)	544
Bsu36I	CCTNAGG	2 cut(s)	1017, 1574
BsuI	GTATCC	1 cut(s)	1331
BsuRI	GGCC	9 cut(s)	156, 546, 562, 705, 909, 950, 1009, 1175, 1460
BtgI	CCRYGG	2 cut(s)	552, 1224
BtrI	CACGTC	1 cut(s)	772
BtsCI	GGATG	9 cut(s)	255, 289, 478, 570, 914, 950, 1200, 1339, 1431
BtsI	GCAGTG	2 cut(s)	384, 804
BtsIMutI	CAGTG	3 cut(s)	60, 384, 804
BveI	ACCTGC	1 cut(s)	1570
Cac8I	GCNNGC	7 cut(s)	138, 406, 997, 1097, 1155, 1424, 1567
CaiI	CAGNNNCTG	1 cut(s)	660
CciNI	GCGGCCGC	1 cut(s)	544
CfoI	GCGC	4 cut(s)	822, 1001, 1524, 1655
Cfr10I	RCCGGY	1 cut(s)	872
Cfr13I	GGNCC	6 cut(s)	58, 167, 561, 908, 948, 1377
Cfr9I	CCCGGG	1 cut(s)	1367
CseI	GACGC	1 cut(s)	1540
Csp6I	GTAC	8 cut(s)	373, 502, 652, 1027, 1056, 1162, 1192, 1585
CviAII	CATG	9 cut(s)	90, 246, 301, 370, 391, 553, 628, 835, 1323
CviQI	GTAC	8 cut(s)	373, 502, 652, 1027, 1056, 1162, 1192, 1585
DdeI	CTNAG	4 cut(s)	1017, 1158, 1574, 1635
DpnI	GATC	8 cut(s)	354, 359, 465, 491, 698, 1043, 1067, 1289
DpnII	GATC	8 cut(s)	352, 357, 463, 489, 696, 1041, 1065, 1287
EaeI	YGGCCR	4 cut(s)	154, 544, 1173, 1458
EagI	CGGCCG	1 cut(s)	544
Eam1104I	CTCTTC	2 cut(s)	626, 1304
EarI	CTCTTC	2 cut(s)	626, 1304
EciI	GGCGGA	1 cut(s)	1066
Ecl136II	GAGCTC	1 cut(s)	925
EclXI	CGGCCG	1 cut(s)	544
Eco130I	CCWWGG	5 cut(s)	292, 552, 594, 700, 1242
Eco147I	AGGCCT	1 cut(s)	1009
Eco24I	GRGCYC	4 cut(s)	618, 927, 1025, 1368
Eco31I	GGTCTC	1 cut(s)	960
Eco47I	GGWCC	3 cut(s)	58, 167, 1377
Eco47III	AGCGCT	2 cut(s)	1000, 1654
Eco52I	CGGCCG	1 cut(s)	544
Eco53kI	GAGCTC	1 cut(s)	925
Eco57I	CTGAAG	1 cut(s)	676
Eco81I	CCTNAGG	2 cut(s)	1017, 1574
Eco88I	CYCGRG	3 cut(s)	467, 920, 1367
Eco91I	GGTNACC	1 cut(s)	1237
EcoICRI	GAGCTC	1 cut(s)	925
EcoO109I	RGGNCCY	1 cut(s)	167
EcoO65I	GGTNACC	1 cut(s)	1237
EcoRI	GAATTC	1 cut(s)	271
EcoRII	CCWGG	7 cut(s)	60, 163, 316, 634, 950, 1004, 1451
EcoT14I	CCWWGG	5 cut(s)	292, 552, 594, 700, 1242
EcoT38I	GRGCYC	4 cut(s)	618, 927, 1025, 1368
ErhI	CCWWGG	5 cut(s)	292, 552, 594, 700, 1242
Esp3I	CGTCTC	1 cut(s)	594
FaeI	CATG	9 cut(s)	93, 249, 304, 373, 394, 556, 631, 838, 1326
FalI	AAGNNNNNCTT	2 cut(s)	1226, 1258
FaqI	GGGAC	5 cut(s)	113, 299, 437, 748, 1592
FatI	CATG	9 cut(s)	89, 245, 300, 369, 390, 552, 627, 834, 1322
Fnu4HI	GCNGC	7 cut(s)	253, 402, 409, 544, 547, 1002, 1458
FokI	GGATG	9 cut(s)	242, 296, 485, 557, 901, 957, 1187, 1346, 1418
FriOI	GRGCYC	4 cut(s)	618, 927, 1025, 1368
Fsp4HI	GCNGC	7 cut(s)	253, 402, 409, 544, 547, 1002, 1458
GlaI	GCGC	4 cut(s)	821, 1000, 1523, 1654
GluI	GCNGC	7 cut(s)	253, 402, 409, 544, 547, 1002, 1458
GsaI	CCCAGC	2 cut(s)	136, 1220
HaeII	RGCGCY	3 cut(s)	1002, 1525, 1656
HaeIII	GGCC	9 cut(s)	156, 546, 562, 705, 909, 950, 1009, 1175, 1460
HapII	CCGG	5 cut(s)	42, 873, 1368, 1485, 1514
HgaI	GACGC	1 cut(s)	1540
HhaI	GCGC	4 cut(s)	822, 1001, 1524, 1655
Hin1II	CATG	9 cut(s)	93, 249, 304, 373, 394, 556, 631, 838, 1326
Hin6I	GCGC	4 cut(s)	820, 999, 1522, 1653
HinP1I	GCGC	4 cut(s)	820, 999, 1522, 1653
HincII	GTYRAC	3 cut(s)	1072, 1261, 1470
HindII	GTYRAC	3 cut(s)	1072, 1261, 1470
HindIII	AAGCTT	1 cut(s)	716
HinfI	GANTC	6 cut(s)	126, 420, 761, 852, 1119, 1133
HpaII	CCGG	5 cut(s)	42, 873, 1368, 1485, 1514
HphI	GGTGA	9 cut(s)	91, 322, 616, 847, 862, 1042, 1099, 1231, 1551
Hpy166II	GTNNAC	7 cut(s)	419, 504, 775, 1072, 1261, 1377, 1470
Hpy188I	TCNGA	5 cut(s)	125, 357, 1138, 1507, 1532
Hpy8I	GTNNAC	7 cut(s)	419, 504, 775, 1072, 1261, 1377, 1470
Hpy99I	CGWCG	2 cut(s)	569, 1358
HpyAV	CCTTC	7 cut(s)	552, 725, 985, 1279, 1354, 1415, 1483
HpyCH4III	ACNGT	7 cut(s)	434, 484, 745, 865, 902, 1225, 1539
HpyCH4IV	ACGT	7 cut(s)	210, 666, 771, 1128, 1254, 1497, 1545
HpyCH4V	TGCA	5 cut(s)	394, 663, 775, 914, 1565
HpyF10VI	GCNNNNNNNGC	5 cut(s)	669, 781, 888, 1001, 1101
HpyF3I	CTNAG	4 cut(s)	1017, 1158, 1574, 1635
HpySE526I	ACGT	7 cut(s)	210, 666, 771, 1128, 1254, 1497, 1545
Hsp92II	CATG	9 cut(s)	93, 249, 304, 373, 394, 556, 631, 838, 1326
HspAI	GCGC	4 cut(s)	820, 999, 1522, 1653
Kzo9I	GATC	8 cut(s)	352, 357, 463, 489, 696, 1041, 1065, 1287
LguI	GCTCTTC	1 cut(s)	1304
LmnI	GCTCC	7 cut(s)	249, 363, 740, 930, 940, 1104, 1574
Lsp1109I	GCAGC	2 cut(s)	413, 988
LweI	GCATC	4 cut(s)	264, 435, 579, 923
MaeII	ACGT	7 cut(s)	210, 666, 771, 1128, 1254, 1497, 1545
MaeIII	GTNAC	8 cut(s)	97, 382, 512, 802, 902, 1237, 1274, 1557
MalI	GATC	8 cut(s)	354, 359, 465, 491, 698, 1043, 1067, 1289
MbiI	CCGCTC	2 cut(s)	252, 521
MboI	GATC	8 cut(s)	352, 357, 463, 489, 696, 1041, 1065, 1287
MboII	GAAGA	8 cut(s)	76, 80, 205, 280, 613, 652, 1177, 1321
MflI	RGATCY	1 cut(s)	696
MhlI	GDGCHC	8 cut(s)	49, 308, 618, 777, 870, 927, 1025, 1368
MlsI	TGGCCA	1 cut(s)	156
MluCI	AATT	3 cut(s)	141, 271, 530
MluNI	TGGCCA	1 cut(s)	156
MlyI	GAGTC	4 cut(s)	135, 414, 770, 1128
MmeI	TCCRAC	2 cut(s)	213, 1485
Mox20I	TGGCCA	1 cut(s)	156
MroXI	GAANNNNTTC	1 cut(s)	212
MscI	TGGCCA	1 cut(s)	156
MseI	TTAA	2 cut(s)	713, 1657
MslI	CAYNNNNRTG	4 cut(s)	389, 485, 1332, 1436
Msp20I	TGGCCA	1 cut(s)	156
MspA1I	CMGCKG	2 cut(s)	543, 981
MspI	CCGG	5 cut(s)	42, 873, 1368, 1485, 1514
Mva1269I	GAATGC	1 cut(s)	1314
MvaI	CCWGG	7 cut(s)	62, 165, 318, 636, 952, 1006, 1453
MwoI	GCNNNNNNNGC	5 cut(s)	669, 781, 888, 1001, 1101
NciI	CCSGG	3 cut(s)	43, 1368, 1369
NcoI	CCATGG	1 cut(s)	552
NdeII	GATC	8 cut(s)	352, 357, 463, 489, 696, 1041, 1065, 1287
NlaIII	CATG	9 cut(s)	93, 249, 304, 373, 394, 556, 631, 838, 1326
NmuCI	GTSAC	7 cut(s)	97, 382, 512, 802, 1237, 1274, 1557
NotI	GCGGCCGC	1 cut(s)	544
NspI	RCATGY	1 cut(s)	373
PaqCI	CACCTGC	1 cut(s)	1570
PceI	AGGCCT	1 cut(s)	1009
PciI	ACATGT	1 cut(s)	369
PciSI	GCTCTTC	1 cut(s)	1304
PcsI	WCGNNNNNNNCGW	4 cut(s)	207, 609, 807, 1134
PctI	GAATGC	1 cut(s)	1314
PdmI	GAANNNNTTC	1 cut(s)	212
PfeI	GAWTC	2 cut(s)	852, 1133
PflFI	GACNNNGTC	2 cut(s)	1181, 1235
PflMI	CCANNNNNTGG	1 cut(s)	1452
PkrI	GCNGC	7 cut(s)	254, 403, 410, 545, 548, 1003, 1459
PleI	GAGTC	4 cut(s)	134, 414, 769, 1127
PpsI	GAGTC	4 cut(s)	134, 414, 769, 1127
Ppu21I	YACGTR	2 cut(s)	1129, 1546
PpuMI	RGGWCCY	1 cut(s)	167
PscI	ACATGT	1 cut(s)	369
Psp124BI	GAGCTC	1 cut(s)	927
Psp1406I	AACGTT	1 cut(s)	1254
Psp5II	RGGWCCY	1 cut(s)	167
Psp6I	CCWGG	7 cut(s)	60, 163, 316, 634, 950, 1004, 1451
PspEI	GGTNACC	1 cut(s)	1237
PspFI	CCCAGC	2 cut(s)	132, 1216
PspGI	CCWGG	7 cut(s)	60, 163, 316, 634, 950, 1004, 1451
PspPI	GGNCC	6 cut(s)	58, 167, 561, 908, 948, 1377
PspPPI	RGGWCCY	1 cut(s)	167
PstNI	CAGNNNCTG	1 cut(s)	660
PsuI	RGATCY	1 cut(s)	696
PsyI	GACNNNGTC	2 cut(s)	1181, 1235
RsaI	GTAC	8 cut(s)	374, 503, 653, 1028, 1057, 1163, 1193, 1586
RsaNI	GTAC	8 cut(s)	373, 502, 652, 1027, 1056, 1162, 1192, 1585
RseI	CAYNNNNRTG	4 cut(s)	389, 485, 1332, 1436
SacI	GAGCTC	1 cut(s)	927
SapI	GCTCTTC	1 cut(s)	1304
SaqAI	TTAA	2 cut(s)	713, 1657
SatI	GCNGC	7 cut(s)	253, 402, 409, 544, 547, 1002, 1458
Sau3AI	GATC	8 cut(s)	352, 357, 463, 489, 696, 1041, 1065, 1287
Sau96I	GGNCC	6 cut(s)	58, 167, 561, 908, 948, 1377
ScaI	AGTACT	1 cut(s)	1163
SchI	GAGTC	4 cut(s)	135, 414, 770, 1128
SduI	GDGCHC	8 cut(s)	49, 308, 618, 777, 870, 927, 1025, 1368
SfaNI	GCATC	4 cut(s)	264, 435, 579, 923
SfcI	CTRYAG	1 cut(s)	939
SinI	GGWCC	3 cut(s)	58, 167, 1377
SmaI	CCCGGG	1 cut(s)	1369
SmiMI	CAYNNNNRTG	4 cut(s)	389, 485, 1332, 1436
SmlI	CTYRAG	2 cut(s)	524, 1148
SmoI	CTYRAG	2 cut(s)	524, 1148
Sse9I	AATT	3 cut(s)	141, 271, 530
SseBI	AGGCCT	1 cut(s)	1009
SstI	GAGCTC	1 cut(s)	927
StuI	AGGCCT	1 cut(s)	1009
StyI	CCWWGG	5 cut(s)	292, 552, 594, 700, 1242
TaaI	ACNGT	7 cut(s)	434, 484, 745, 865, 902, 1225, 1539
TaiI	ACGT	7 cut(s)	213, 669, 774, 1131, 1257, 1500, 1548
TaqI	TCGA	5 cut(s)	225, 723, 845, 1082, 1476
TasI	AATT	3 cut(s)	141, 271, 530
TatI	WGTACW	4 cut(s)	372, 651, 1161, 1584
TauI	GCSGC	5 cut(s)	255, 411, 546, 549, 1460
TfiI	GAWTC	2 cut(s)	852, 1133
Tru1I	TTAA	2 cut(s)	713, 1657
Tru9I	TTAA	2 cut(s)	713, 1657
TscAI	CASTG	3 cut(s)	60, 391, 811
TseFI	GTSAC	7 cut(s)	97, 382, 512, 802, 1237, 1274, 1557
TseI	GCWGC	2 cut(s)	401, 1001
Tsp45I	GTSAC	7 cut(s)	97, 382, 512, 802, 1237, 1274, 1557
TspDTI	ATGAA	4 cut(s)	489, 644, 909, 1224
TspGWI	ACGGA	6 cut(s)	120, 959, 1050, 1131, 1262, 1428
TspMI	CCCGGG	1 cut(s)	1367
TspRI	CASTG	3 cut(s)	60, 391, 811
Tth111I	GACNNNGTC	2 cut(s)	1181, 1235
Van91I	CCANNNNNTGG	1 cut(s)	1452
VneI	GTGCAC	1 cut(s)	773
VpaK11BI	GGWCC	3 cut(s)	58, 167, 1377
XapI	RAATTY	1 cut(s)	271
XceI	RCATGY	1 cut(s)	373
XmaI	CCCGGG	1 cut(s)	1367
XmnI	GAANNNNTTC	1 cut(s)	212
ZrmI	AGTACT	1 cut(s)	1163

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.