Rw7G026320

L-ascorbate oxidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001996
Physical Location & Seq
Forward (+)
33405046 .. 33406134
1089 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G026320.1

Sequence Viewer

Length: 768 bp
ATGAAATTGCTGCTGTTGTGCCTCTCGATTGGCGTAATCTCAGTTATTCATGGTAAGGTAAGGGTACCTTTTCTTCACATGGAATTCCCCCAGCAAGGCATTCTCATCAACGGCGAGTTTCCGGGACCTAACATTAACTCCACCACCAACAACAACATCGTCATCAATGTCTTCAACAACCTTGATGAACCATTGATGTTCACATGGAATGGAATCCAACAGAAGAAGAATTCATGGCAAGATGGAACACCAGGGACTATGTGCCCTATTCCTCCTGGTCAGAACTACACATACCACTTCCAAGTGAAGGACCAGATTGGAAGCTATTTCTACTATCCCATCACTGCCATGCACAGAGCAGGTGGTGCCTTGCCAATTATCTTTGAGAACCACGAGAAGAGCATGCAGTCATGGCATCTGGATGGATACGCTTTCTTTCCCGCTGCCATCGAGTTTGGGAGGTGGGCACCAGAGAAAAGAAGGAACTACAACCTTCTTGACGCCATAAGCCGACACACCGTCCACGTGTATCCCCGCTCATGGGCCGCCATTATGTGGACATTCAACAACGCTGGAATGTGGAACTTGAGGTCAGCACAGCCGGAGAGGGCGTACCTAGAACAACAGCTCTACGTGAGTGTTGAAAATGTCGCACGCTCTCTCAGGGACGAATACATGCTGCTACACCAGACACCTCTCTGTGGCATCGTCAAGGGTCTCCCTCAACCCCCTCCATACGGCCCTTCAAGTATCAATACGTGTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

255

Amino Acids

29.12

Weight (kDa)

8.31

Isoelectric Point (pI)

52.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 30 - 127 1.5e-35 Multicopper oxidase
Cu-oxidase_2 PF07731 122 - 216 6.7e-21 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 350
Acc36I ACCTGC 1 cut(s) 350
Acc65I GGTACC 1 cut(s) 64
AccB1I GGYRCC 3 cut(s) 64, 365, 466
AccB7I CCANNNNNTGG 1 cut(s) 555
AccBSI CCGCTC 1 cut(s) 537
AciI CCGC 3 cut(s) 441, 535, 546
AcsI RAATTY 2 cut(s) 83, 229
AcvI CACGTG 1 cut(s) 526
AcyI GRCGYC 1 cut(s) 501
AfaI GTAC 2 cut(s) 66, 614
AfiI CCNNNNNNNGG 7 cut(s) 95, 307, 540, 541, 555, 701, 737
AflIII ACRYGT 2 cut(s) 525, 758
AgsI TTSAA 4 cut(s) 175, 565, 644, 747
AhdI GACNNNNNGTC 1 cut(s) 518
AjnI CCWGG 2 cut(s) 250, 274
AluBI AGCT 2 cut(s) 324, 628
AluI AGCT 2 cut(s) 324, 628
Alw26I GTCTC 1 cut(s) 722
AoxI GGCC 2 cut(s) 543, 739
ApeKI GCWGC 3 cut(s) 10, 443, 679
ApoI RAATTY 2 cut(s) 83, 229
Asp718I GGTACC 1 cut(s) 64
AspS9I GGNCC 4 cut(s) 125, 310, 543, 740
AsuC2I CCSGG 1 cut(s) 123
AvaII GGWCC 2 cut(s) 125, 310
BaeGI GKGCMC 2 cut(s) 266, 469
BanI GGYRCC 3 cut(s) 64, 365, 466
BarI GAAGNNNNNNTAC 2 cut(s) 57, 89
BauI CACGAG 1 cut(s) 392
BbrPI CACGTG 1 cut(s) 526
BbsI GAAGAC 1 cut(s) 163
BbvI GCAGC 2 cut(s) 430, 666
BccI CCATC 4 cut(s) 236, 347, 416, 455
BceAI ACGGC 2 cut(s) 127, 754
BciT130I CCWGG 2 cut(s) 252, 276
BciVI GTATCC 2 cut(s) 419, 540
BcnI CCSGG 1 cut(s) 123
BcoDI GTCTC 1 cut(s) 722
BfaI CTAG 1 cut(s) 617
BfuAI ACCTGC 1 cut(s) 350
BfuI GTATCC 2 cut(s) 419, 540
BisI GCNGC 4 cut(s) 11, 444, 546, 680
BlsI GCNGC 4 cut(s) 12, 445, 547, 681
Bme1390I CCNGG 3 cut(s) 123, 252, 276
Bme18I GGWCC 2 cut(s) 125, 310
BmeRI GACNNNNNGTC 1 cut(s) 518
BmgT120I GGNCC 4 cut(s) 125, 310, 543, 740
BmiI GGNNCC 4 cut(s) 66, 126, 367, 468
BmrFI CCNGG 3 cut(s) 123, 252, 276
BmsI GCATC 2 cut(s) 424, 714
BpiI GAAGAC 1 cut(s) 163
BpuEI CTTGAG 1 cut(s) 607
BpuMI CCSGG 1 cut(s) 123
BsaAI YACGTR 3 cut(s) 526, 634, 759
BsaHI GRCGYC 1 cut(s) 501
BsaI GGTCTC 1 cut(s) 722
BsaJI CCNNGG 1 cut(s) 251
BsaXI ACNNNNNCTCC 4 cut(s) 122, 152, 596, 626
Bsc4I CCNNNNNNNGG 7 cut(s) 95, 307, 540, 541, 555, 701, 737
BseBI CCWGG 2 cut(s) 252, 276
BseDI CCNNGG 1 cut(s) 251
BseGI GGATG 1 cut(s) 427
BseLI CCNNNNNNNGG 7 cut(s) 95, 307, 540, 541, 555, 701, 737
BseMII CTCAG 2 cut(s) 54, 676
BseSI GKGCMC 2 cut(s) 266, 469
BseXI GCAGC 2 cut(s) 430, 666
BseYI CCCAGC 1 cut(s) 90
BshFI GGCC 2 cut(s) 545, 741
BshNI GGYRCC 3 cut(s) 64, 365, 466
BsiSI CCGG 2 cut(s) 122, 602
BslFI GGGAC 3 cut(s) 138, 268, 680
BslI CCNNNNNNNGG 7 cut(s) 95, 307, 540, 541, 555, 701, 737
BsmAI GTCTC 1 cut(s) 722
BsmFI GGGAC 3 cut(s) 138, 268, 680
BsmI GAATGC 1 cut(s) 99
BsnI GGCC 2 cut(s) 545, 741
Bso31I GGTCTC 1 cut(s) 722
Bsp1286I GDGCHC 2 cut(s) 266, 469
BspACI CCGC 3 cut(s) 441, 535, 546
BspANI GGCC 2 cut(s) 545, 741
BspCNI CTCAG 2 cut(s) 53, 675
BspLI GGNNCC 4 cut(s) 66, 126, 367, 468
BspMI ACCTGC 1 cut(s) 350
BspQI GCTCTTC 1 cut(s) 392
BspT107I GGYRCC 3 cut(s) 64, 365, 466
BspTNI GGTCTC 1 cut(s) 722
BsrBI CCGCTC 1 cut(s) 537
BssECI CCNNGG 1 cut(s) 251
BssNI GRCGYC 1 cut(s) 501
BssSI CACGAG 1 cut(s) 392
Bst2BI CACGAG 1 cut(s) 392
Bst2UI CCWGG 2 cut(s) 252, 276
Bst4CI ACNGT 1 cut(s) 520
Bst6I CTCTTC 1 cut(s) 392
BstACI GRCGYC 1 cut(s) 501
BstAPI GCANNNNNTGC 1 cut(s) 365
BstBAI YACGTR 3 cut(s) 526, 634, 759
BstC8I GCNNGC 2 cut(s) 404, 655
BstDEI CTNAG 2 cut(s) 40, 662
BstF5I GGATG 1 cut(s) 427
BstMAI GTCTC 1 cut(s) 722
BstMWI GCNNNNNNNGC 2 cut(s) 365, 412
BstNI CCWGG 2 cut(s) 252, 276
BstNSI RCATGY 2 cut(s) 406, 679
BstSCI CCNGG 3 cut(s) 121, 250, 274
BstSLI GKGCMC 2 cut(s) 266, 469
BstV1I GCAGC 2 cut(s) 430, 666
BstV2I GAAGAC 1 cut(s) 163
BsuI GTATCC 2 cut(s) 419, 540
BsuRI GGCC 2 cut(s) 545, 741
BtsCI GGATG 1 cut(s) 427
BtsI GCAGTG 1 cut(s) 342
BtsIMutI CAGTG 1 cut(s) 342
BveI ACCTGC 1 cut(s) 350
Cac8I GCNNGC 2 cut(s) 404, 655
Cfr13I GGNCC 4 cut(s) 125, 310, 543, 740
CseI GACGC 1 cut(s) 509
Csp6I GTAC 2 cut(s) 65, 613
CviAII CATG 9 cut(s) 50, 79, 204, 234, 349, 403, 411, 540, 676
CviJI RGCY 6 cut(s) 324, 510, 545, 601, 628, 741
CviKI_1 RGCY 6 cut(s) 324, 510, 545, 601, 628, 741
CviQI GTAC 2 cut(s) 65, 613
DdeI CTNAG 2 cut(s) 40, 662
DriI GACNNNNNGTC 1 cut(s) 518
Eam1104I CTCTTC 1 cut(s) 392
Eam1105I GACNNNNNGTC 1 cut(s) 518
EarI CTCTTC 1 cut(s) 392
Eco31I GGTCTC 1 cut(s) 722
Eco47I GGWCC 2 cut(s) 125, 310
Eco72I CACGTG 1 cut(s) 526
EcoO109I RGGNCCY 1 cut(s) 125
EcoRI GAATTC 2 cut(s) 83, 229
EcoRII CCWGG 2 cut(s) 250, 274
FaeI CATG 9 cut(s) 53, 82, 207, 237, 352, 406, 414, 543, 679
FalI AAGNNNNNCTT 2 cut(s) 52, 84
FaqI GGGAC 3 cut(s) 138, 268, 680
FatI CATG 9 cut(s) 49, 78, 203, 233, 348, 402, 410, 539, 675
FauI CCCGC 2 cut(s) 448, 542
Fnu4HI GCNGC 4 cut(s) 11, 444, 546, 680
FokI GGATG 1 cut(s) 434
Fsp4HI GCNGC 4 cut(s) 11, 444, 546, 680
FspBI CTAG 1 cut(s) 617
GluI GCNGC 4 cut(s) 11, 444, 546, 680
GsaI CCCAGC 1 cut(s) 94
HaeIII GGCC 2 cut(s) 545, 741
HapII CCGG 2 cut(s) 122, 602
HgaI GACGC 1 cut(s) 509
Hin1I GRCGYC 1 cut(s) 501
Hin1II CATG 9 cut(s) 53, 82, 207, 237, 352, 406, 414, 543, 679
HinfI GANTC 1 cut(s) 213
HpaII CCGG 2 cut(s) 122, 602
Hpy166II GTNNAC 3 cut(s) 201, 523, 558
Hpy188I TCNGA 1 cut(s) 282
Hpy188III TCNNGA 3 cut(s) 25, 419, 497
Hpy8I GTNNAC 3 cut(s) 201, 523, 558
HpyAV CCTTC 4 cut(s) 301, 474, 503, 753
HpyCH4III ACNGT 1 cut(s) 520
HpyCH4IV ACGT 3 cut(s) 525, 633, 758
HpyCH4V TGCA 2 cut(s) 352, 406
HpyF10VI GCNNNNNNNGC 2 cut(s) 365, 412
HpyF3I CTNAG 2 cut(s) 40, 662
HpySE526I ACGT 3 cut(s) 525, 633, 758
Hsp92I GRCGYC 1 cut(s) 501
Hsp92II CATG 9 cut(s) 53, 82, 207, 237, 352, 406, 414, 543, 679
KpnI GGTACC 1 cut(s) 68
LguI GCTCTTC 1 cut(s) 392
Lsp1109I GCAGC 2 cut(s) 430, 666
LweI GCATC 2 cut(s) 424, 714
MaeI CTAG 1 cut(s) 617
MaeII ACGT 3 cut(s) 525, 633, 758
MbiI CCGCTC 1 cut(s) 537
MboII GAAGA 5 cut(s) 65, 163, 235, 238, 409
MhlI GDGCHC 2 cut(s) 266, 469
MluCI AATT 4 cut(s) 5, 83, 229, 375
MmeI TCCRAC 1 cut(s) 241
MnlI CCTC 8 cut(s) 32, 282, 453, 582, 600, 705, 732, 741
MseI TTAA 1 cut(s) 135
MslI CAYNNNNRTG 2 cut(s) 347, 420
MspA1I CMGCKG 1 cut(s) 443
MspI CCGG 2 cut(s) 122, 602
MspR9I CCNGG 3 cut(s) 123, 252, 276
Mva1269I GAATGC 1 cut(s) 99
MvaI CCWGG 2 cut(s) 252, 276
MwoI GCNNNNNNNGC 2 cut(s) 365, 412
NciI CCSGG 1 cut(s) 123
NlaIII CATG 9 cut(s) 53, 82, 207, 237, 352, 406, 414, 543, 679
NlaIV GGNNCC 4 cut(s) 66, 126, 367, 468
NspI RCATGY 2 cut(s) 406, 679
PaeI GCATGC 1 cut(s) 406
PaqCI CACCTGC 1 cut(s) 350
PciSI GCTCTTC 1 cut(s) 392
PctI GAATGC 1 cut(s) 99
PfeI GAWTC 1 cut(s) 213
PflMI CCANNNNNTGG 1 cut(s) 555
PfoI TCCNGGA 1 cut(s) 121
PkrI GCNGC 4 cut(s) 12, 445, 547, 681
PmaCI CACGTG 1 cut(s) 526
PmlI CACGTG 1 cut(s) 526
Ppu21I YACGTR 3 cut(s) 526, 634, 759
PpuMI RGGWCCY 1 cut(s) 125
Psp5II RGGWCCY 1 cut(s) 125
Psp6I CCWGG 2 cut(s) 250, 274
PspCI CACGTG 1 cut(s) 526
PspFI CCCAGC 1 cut(s) 90
PspGI CCWGG 2 cut(s) 250, 274
PspN4I GGNNCC 4 cut(s) 66, 126, 367, 468
PspPI GGNCC 4 cut(s) 125, 310, 543, 740
PspPPI RGGWCCY 1 cut(s) 125
PsrI GAACNNNNNNTAC 2 cut(s) 275, 307
RsaI GTAC 2 cut(s) 66, 614
RsaNI GTAC 2 cut(s) 65, 613
RseI CAYNNNNRTG 2 cut(s) 347, 420
SapI GCTCTTC 1 cut(s) 392
SaqAI TTAA 1 cut(s) 135
SatI GCNGC 4 cut(s) 11, 444, 546, 680
Sau96I GGNCC 4 cut(s) 125, 310, 543, 740
ScrFI CCNGG 3 cut(s) 123, 252, 276
SduI GDGCHC 2 cut(s) 266, 469
SfaNI GCATC 2 cut(s) 424, 714
SinI GGWCC 2 cut(s) 125, 310
SmiMI CAYNNNNRTG 2 cut(s) 347, 420
SmlI CTYRAG 1 cut(s) 586
SmoI CTYRAG 1 cut(s) 586
SphI GCATGC 1 cut(s) 406
Sse9I AATT 4 cut(s) 5, 83, 229, 375
SsiI CCGC 3 cut(s) 441, 535, 546
SspMI CTAG 1 cut(s) 617
StyD4I CCNGG 3 cut(s) 121, 250, 274
TaaI ACNGT 1 cut(s) 520
TaiI ACGT 3 cut(s) 528, 636, 761
TaqI TCGA 2 cut(s) 26, 450
TasI AATT 4 cut(s) 5, 83, 229, 375
TauI GCSGC 1 cut(s) 548
TfiI GAWTC 1 cut(s) 213
Tru1I TTAA 1 cut(s) 135
Tru9I TTAA 1 cut(s) 135
TscAI CASTG 1 cut(s) 349
TseI GCWGC 3 cut(s) 10, 443, 679
TspDTI ATGAA 4 cut(s) 17, 38, 201, 222
TspRI CASTG 1 cut(s) 349
Van91I CCANNNNNTGG 1 cut(s) 555
VpaK11BI GGWCC 2 cut(s) 125, 310
XapI RAATTY 2 cut(s) 83, 229
XceI RCATGY 2 cut(s) 406, 679
XspI CTAG 1 cut(s) 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.