Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0001996

Physical Location & Seq

Genomic Coordinates Reverse (-)

23050 .. 25811

Gene Structure 2762 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1000500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1656 bp

ATGTCTGGGATGATTGTTATGCTGCTGTTATGCCTCTCAGTGGGGACAATTTCGGTAGTCAACTGTGGAGATCCTACCATTTTCTTCACATGGAATGTGACCTACGGAACCCTCTCACCCCTAGGAGTTCCCCAGCAAGTCATTCTCATAAATGGCCAATTTCCTGGACCCAACATCAACTCCACCACCAACAACAACGTTATCGTCAACGTCTTCAATAACCTTGACGAACCATTCCTCATCACATGGGTGGGTGTCCAGCAGAGGAAGAATCCATGGCAAGATGGAGTTCTTGGAACCATGTGCCCCATCCCCCCCGGTACAAACTACACCTACAAATTCCAAGTCAAGGATCAGATTGGGAGTTTCATGTACTACCCTGCAACGGCCATGCATAGGGCAGCCGGTGCCTTTGGAGGCCTCCGCATCAACAGCCGTAATTTAATTCCCGTTCCTTACGCTGCTCCCGAGGATGACTACACTGTCATAATCGGTGACTGGTACACCAAAAGCCACAGCCAACTTAGGAAATTCTTGGACAGCGGCCGTAGCATGGCCAGGCCTGACGGCGTCCTTATCAACGGCAAATCAGCAAAAGGAGATGGCAAGGACGAGCCCCTCTTCACCATGAAGGCTGGCAAGACTTACAAGTACAGGATCTGCAACGTGGGGCTCAAGAACTCCCTCAACTTTAGGATCCAAGGCCACCAAATGAAGCTTGTCGAGATGGAGGGCTCCCACACCGTCCAAAACACCTATGATTCGCTTGATGTGCATATTGGCCAATGTTTCTCTATGCTTGTCACTGCCGACAAGGAACCCAAGGACTACTACATGGTGGCTTCCACCCGATTCACCAAGAGCGTGCTCACCGGCAAAGGCATCATCAGATACGAAAATGGTAAGGGACCTGTCTCCCCTGAAGTTCCCAAGGCTCCTGTGGGATGGGCCTGGTCTCTCAACCAGTTCCGTTCCTTTCGCTGGAACCTCACTGCCAGCGCAGCTAGGCCTAACCCTCAGGGATCCTACCACTATGGAAAGATTAACATCACCCGCACCATCAAGCTCATCAACTCTGCTAGCAAGGCCGACGGGAAGCTTCGCTATGCCATCAACGGAATCTCCCACGTGGACCCTGAAACCCCACTCAAGTTGGCCGACTACTATGGAATCCGTGAGAAGGTCTTCAAGTTCGACACCATGAAGGTTGATCCCCAATCACCTGCAGAAAAGATCACCATGGAACCCAATGTTCTCAATATAACCATAAAAGACTTTGTGGAGATCATCTTTGAGAACCCGGAGAAGAGCCTCCAGACTTGGCATTTGAACGGATACGCCTTTTTCGCCGTCGCCGTGGAGCCCGGGAAGTGGACCCCAGAAAAGAGGAAGAACTACAACCTTCTCGACGCCATGAGCAGGCACACCATCCAAGTGTTCCCCAAATCTTGGGCTGCCATCCTATTAACATTCGACAACGCTGGAATGTGGAATCTTAGGTCAGAGCAGCCGGAGAGGCGCTACCTAGGACAACAGCTCTACGTGGGAGTTGAAAATATTAAACGCTCCCTCAGGAACGAGTACAACATGCCAGACAACGCCCTTGTCTGTGGCCTCGTCAAGGGCATGCCTATGCCCCCGCCTTACAGCACGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00053 ko01100 map00053 map01100

Pfam Domains

Protein Families

Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

552

Amino Acids

61.91

Weight (kDa)

9.29

Isoelectric Point (pI)

39.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cu-oxidase_3	PF07732	32 - 147	3.3e-41	Multicopper oxidase
Cu-oxidase	PF00394	158 - 300	2.9e-43	Multicopper oxidase
Cu-oxidase_2	PF07731	381 - 518	5.4e-27	Multicopper oxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001996)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55560 AT1G55570 AT3G13390 AT3G13400 AT3G13400
fragaria_vesca	FvH4_3g46080 FvH4_6g20700
malus_domestica	MD03G1000200.v1.1 MD03G1000500.v1.1 MD11G1000300.v1.1 MD11G1000400.v1.1
prunus_persica	Prupe.6G000400_v2.0.a1 Prupe.6G000500_v2.0.a1
pyrus_communis	pycom03g00050 pycom11g00060
rosa_chinensis	RchiOBHm_Chr3g0476601 RchiOBHm_Chr3g0476621 RchiOBHm_Chr5g0084141
rosa_laevigata	RLG00000001981 RLG00000001982 RLG00000023759 RLG00000037086
rosa_multiflora	Rmu_co7980006.1_g000001 Rmu_sc0008241.1_g000008 Rmu_sc0008241.1_g000011 Rmu_sc0014688.1_g000001 Rmu_sc0035447.1_g000001
rosa_roxburghii	Rroxscaffold_1G00000500 Rroxscaffold_6G00405150 Rroxscaffold_6G00405170
rosa_rugosa	Rorug03G0155600 Rorug03G0155900 Rorug05G0489500
rosa_samantha	Rh3AG206200 Rh3CG232600 Rh3DG232400 Rh3DG232600 Rh5AG542100 Rh5CG589700 Rh5DG576100 Rh7DG309900
rosa_wichuraiana	Rw3G018780 Rw3G018790 Rw5G050430 Rw7G026320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1231
AasI	GACNNNNNNGTC	1 cut(s)	482
Acc36I	ACCTGC	1 cut(s)	1231
AccB1I	GGYRCC	1 cut(s)	407
AccB7I	CCANNNNNTGG	1 cut(s)	1449
AciI	CCGC	4 cut(s)	424, 543, 1054, 1640
AclI	AACGTT	1 cut(s)	198
AclWI	GGATC	8 cut(s)	65, 360, 665, 691, 704, 1017, 1030, 1205
AcoI	YGGCCR	6 cut(s)	154, 387, 544, 555, 781, 1155
AcsI	RAATTY	2 cut(s)	338, 530
AcuI	CTGAAG	1 cut(s)	942
AcvI	CACGTG	1 cut(s)	1129
AcyI	GRCGYC	2 cut(s)	570, 1410
AfaI	GTAC	5 cut(s)	322, 374, 503, 653, 1583
AgsI	TTSAA	4 cut(s)	217, 1189, 1330, 1553
AjnI	CCWGG	3 cut(s)	163, 557, 950
AleI	CACNNNNGTG	1 cut(s)	248
AluBI	AGCT	5 cut(s)	718, 1004, 1066, 1099, 1537
AluI	AGCT	5 cut(s)	718, 1004, 1066, 1099, 1537
Alw21I	GWGCWC	1 cut(s)	870
Alw26I	GTCTC	2 cut(s)	919, 960
AlwI	GGATC	8 cut(s)	65, 360, 665, 691, 704, 1017, 1030, 1205
AlwNI	CAGNNNCTG	1 cut(s)	660
Ama87I	CYCGRG	2 cut(s)	467, 1364
ApeKI	GCWGC	6 cut(s)	22, 401, 461, 1001, 1454, 1507
ApoI	RAATTY	2 cut(s)	338, 530
ArsI	GACNNNNNNTTYG	4 cut(s)	330, 362, 1309, 1341
Asp700I	GAANNNNTTC	1 cut(s)	1184
AspA2I	CCTAGG	2 cut(s)	121, 1525
AspLEI	GCGC	2 cut(s)	1001, 1521
AspS9I	GGNCC	5 cut(s)	167, 908, 948, 1132, 1374
AsuC2I	CCSGG	4 cut(s)	318, 1301, 1365, 1366
AsuHPI	GGTGA	8 cut(s)	108, 506, 616, 847, 862, 1042, 1212, 1228
AsuNHI	GCTAGC	1 cut(s)	1079
AvaI	CYCGRG	2 cut(s)	467, 1364
AvaII	GGWCC	4 cut(s)	167, 908, 1132, 1374
AvrII	CCTAGG	2 cut(s)	121, 1525
AxyI	CCTNAGG	2 cut(s)	1017, 1571
BaeGI	GKGCMC	1 cut(s)	308
BalI	TGGCCA	3 cut(s)	156, 557, 783
BamHI	GGATCC	2 cut(s)	696, 1022
BanI	GGYRCC	1 cut(s)	407
BanII	GRGCYC	4 cut(s)	618, 675, 737, 1365
BbrPI	CACGTG	1 cut(s)	1129
BbsI	GAAGAC	2 cut(s)	205, 1177
Bbv12I	GWGCWC	1 cut(s)	870
BbvI	GCAGC	6 cut(s)	9, 413, 448, 1013, 1441, 1519
BccI	CCATC	9 cut(s)	278, 317, 596, 721, 939, 1067, 1118, 1436, 1466
BceAI	ACGGC	7 cut(s)	402, 420, 531, 583, 598, 1334, 1340
BciT130I	CCWGG	3 cut(s)	165, 559, 952
BciVI	GTATCC	1 cut(s)	1328
BcnI	CCSGG	4 cut(s)	318, 1301, 1365, 1366
BcoDI	GTCTC	2 cut(s)	919, 960
BfaI	CTAG	4 cut(s)	122, 1005, 1080, 1526
BfmI	CTRYAG	1 cut(s)	1224
BfoI	RGCGCY	1 cut(s)	1522
BfuAI	ACCTGC	1 cut(s)	1231
BfuI	GTATCC	1 cut(s)	1328
BglI	GCCNNNNNGGC	1 cut(s)	1516
BisI	GCNGC	7 cut(s)	23, 402, 462, 544, 1002, 1455, 1508
BlnI	CCTAGG	2 cut(s)	121, 1525
BlsI	GCNGC	7 cut(s)	24, 403, 463, 545, 1003, 1456, 1509
Bme1390I	CCNGG	7 cut(s)	165, 318, 559, 952, 1301, 1365, 1366
Bme18I	GGWCC	4 cut(s)	167, 908, 1132, 1374
BmeT110I	CYCGRG	2 cut(s)	467, 1364
BmgT120I	GGNCC	5 cut(s)	167, 908, 948, 1132, 1374
BmrFI	CCNGG	7 cut(s)	165, 318, 559, 952, 1301, 1365, 1366
BmsI	GCATC	2 cut(s)	435, 891
BmtI	GCTAGC	1 cut(s)	1083
BpiI	GAAGAC	2 cut(s)	205, 1177
BpmI	CTGGAG	1 cut(s)	1298
BpuEI	CTTGAG	2 cut(s)	659, 1133
BpuMI	CCSGG	4 cut(s)	318, 1301, 1365, 1366
BsaAI	YACGTR	3 cut(s)	1129, 1543, 1653
BsaBI	GATNNNNATC	1 cut(s)	1046
BsaHI	GRCGYC	2 cut(s)	570, 1410
BsaI	GGTCTC	1 cut(s)	960
BsaXI	ACNNNNNCTCC	4 cut(s)	164, 194, 1106, 1136
Bse118I	RCCGGY	2 cut(s)	404, 872
Bse1I	ACTGG	2 cut(s)	503, 964
Bse21I	CCTNAGG	2 cut(s)	1017, 1571
Bse8I	GATNNNNATC	1 cut(s)	1046
BseBI	CCWGG	3 cut(s)	165, 559, 952
BseGI	GGATG	6 cut(s)	15, 309, 478, 950, 1428, 1458
BseJI	GATNNNNATC	1 cut(s)	1046
BseMII	CTCAG	3 cut(s)	51, 1031, 1585
BseNI	ACTGG	2 cut(s)	503, 964
BseSI	GKGCMC	1 cut(s)	308
BseX3I	CGGCCG	1 cut(s)	544
BseXI	GCAGC	6 cut(s)	9, 413, 448, 1013, 1441, 1519
BseYI	CCCAGC	1 cut(s)	132
Bsh1285I	CGRYCG	1 cut(s)	547
BshNI	GGYRCC	1 cut(s)	407
BsiEI	CGRYCG	1 cut(s)	547
BsiHKAI	GWGCWC	1 cut(s)	870
BsiHKCI	CYCGRG	2 cut(s)	467, 1364
BsiSI	CCGG	6 cut(s)	318, 405, 873, 1301, 1365, 1511
BslFI	GGGAC	2 cut(s)	58, 921
BsmAI	GTCTC	2 cut(s)	919, 960
BsmFI	GGGAC	2 cut(s)	58, 921
Bso31I	GGTCTC	1 cut(s)	960
BsoBI	CYCGRG	2 cut(s)	467, 1364
Bsp1286I	GDGCHC	6 cut(s)	308, 618, 675, 737, 870, 1365
Bsp143I	GATC	8 cut(s)	70, 352, 657, 696, 1022, 1210, 1233, 1284
Bsp19I	CCATGG	2 cut(s)	275, 1239
BspACI	CCGC	4 cut(s)	424, 543, 1054, 1640
BspCNI	CTCAG	3 cut(s)	50, 1030, 1584
BspMAI	CTGCAG	1 cut(s)	1228
BspMI	ACCTGC	1 cut(s)	1231
BspOI	GCTAGC	1 cut(s)	1083
BspPI	GGATC	8 cut(s)	65, 360, 665, 691, 704, 1017, 1030, 1205
BspQI	GCTCTTC	1 cut(s)	1301
BspT107I	GGYRCC	1 cut(s)	407
BspTNI	GGTCTC	1 cut(s)	960
BsrFI	RCCGGY	2 cut(s)	404, 872
BsrI	ACTGG	2 cut(s)	503, 964
BssAI	RCCGGY	2 cut(s)	404, 872
BssMI	GATC	8 cut(s)	70, 352, 657, 696, 1022, 1210, 1233, 1284
BssNI	GRCGYC	2 cut(s)	570, 1410
BssT1I	CCWWGG	7 cut(s)	121, 275, 700, 822, 930, 1239, 1525
Bst2UI	CCWGG	3 cut(s)	165, 559, 952
Bst4CI	ACNGT	3 cut(s)	65, 484, 745
Bst6I	CTCTTC	2 cut(s)	626, 1301
BstACI	GRCGYC	2 cut(s)	570, 1410
BstAPI	GCANNNNNTGC	1 cut(s)	407
BstBAI	YACGTR	3 cut(s)	1129, 1543, 1653
BstC8I	GCNNGC	6 cut(s)	637, 866, 997, 1081, 1421, 1628
BstDEI	CTNAG	5 cut(s)	37, 524, 1017, 1496, 1571
BstDSI	CCRYGG	3 cut(s)	275, 1239, 1356
BstENI	CCTNNNNNAGG	1 cut(s)	1619
BstF5I	GGATG	6 cut(s)	15, 309, 478, 950, 1428, 1458
BstH2I	RGCGCY	1 cut(s)	1522
BstHHI	GCGC	2 cut(s)	1001, 1521
BstKTI	GATC	8 cut(s)	73, 355, 660, 699, 1025, 1213, 1236, 1287
BstMAI	GTCTC	2 cut(s)	919, 960
BstMBI	GATC	8 cut(s)	70, 352, 657, 696, 1022, 1210, 1233, 1284
BstMCI	CGRYCG	1 cut(s)	547
BstMWI	GCNNNNNNNGC	8 cut(s)	407, 432, 549, 772, 1001, 1085, 1346, 1516
BstNI	CCWGG	3 cut(s)	165, 559, 952
BstNSI	RCATGY	2 cut(s)	1591, 1630
BstSCI	CCNGG	7 cut(s)	163, 316, 557, 950, 1299, 1363, 1364
BstSFI	CTRYAG	1 cut(s)	1224
BstSLI	GKGCMC	1 cut(s)	308
BstV1I	GCAGC	6 cut(s)	9, 413, 448, 1013, 1441, 1519
BstV2I	GAAGAC	2 cut(s)	205, 1177
BstX2I	RGATCY	4 cut(s)	70, 657, 696, 1022
BstXI	CCANNNNNNTGG	1 cut(s)	164
BstYI	RGATCY	4 cut(s)	70, 657, 696, 1022
BstZI	CGGCCG	1 cut(s)	544
Bsu36I	CCTNAGG	2 cut(s)	1017, 1571
BsuI	GTATCC	1 cut(s)	1328
BtgI	CCRYGG	3 cut(s)	275, 1239, 1356
BtsCI	GGATG	6 cut(s)	15, 309, 478, 950, 1428, 1458
BtsI	GCAGTG	2 cut(s)	804, 990
BtsIMutI	CAGTG	4 cut(s)	45, 480, 804, 990
BveI	ACCTGC	1 cut(s)	1231
Cac8I	GCNNGC	6 cut(s)	637, 866, 997, 1081, 1421, 1628
CaiI	CAGNNNCTG	1 cut(s)	660
CfoI	GCGC	2 cut(s)	1001, 1521
Cfr10I	RCCGGY	2 cut(s)	404, 872
Cfr13I	GGNCC	5 cut(s)	167, 908, 948, 1132, 1374
Cfr9I	CCCGGG	1 cut(s)	1364
CseI	GACGC	2 cut(s)	559, 1418
Csp6I	GTAC	5 cut(s)	321, 373, 502, 652, 1582
CspCI	CAANNNNNGTGG	2 cut(s)	1134, 1169
CviQI	GTAC	5 cut(s)	321, 373, 502, 652, 1582
DdeI	CTNAG	5 cut(s)	37, 524, 1017, 1496, 1571
DpnI	GATC	8 cut(s)	72, 354, 659, 698, 1024, 1212, 1235, 1286
DpnII	GATC	8 cut(s)	70, 352, 657, 696, 1022, 1210, 1233, 1284
DrdI	GACNNNNNNGTC	1 cut(s)	482
DseDI	GACNNNNNNGTC	1 cut(s)	482
EaeI	YGGCCR	6 cut(s)	154, 387, 544, 555, 781, 1155
EagI	CGGCCG	1 cut(s)	544
Eam1104I	CTCTTC	2 cut(s)	626, 1301
EarI	CTCTTC	2 cut(s)	626, 1301
EclXI	CGGCCG	1 cut(s)	544
Eco130I	CCWWGG	7 cut(s)	121, 275, 700, 822, 930, 1239, 1525
Eco147I	AGGCCT	3 cut(s)	420, 562, 1009
Eco24I	GRGCYC	4 cut(s)	618, 675, 737, 1365
Eco31I	GGTCTC	1 cut(s)	960
Eco47I	GGWCC	4 cut(s)	167, 908, 1132, 1374
Eco52I	CGGCCG	1 cut(s)	544
Eco57I	CTGAAG	1 cut(s)	942
Eco72I	CACGTG	1 cut(s)	1129
Eco81I	CCTNAGG	2 cut(s)	1017, 1571
Eco88I	CYCGRG	2 cut(s)	467, 1364
EcoNI	CCTNNNNNAGG	1 cut(s)	1619
EcoO109I	RGGNCCY	1 cut(s)	908
EcoRII	CCWGG	3 cut(s)	163, 557, 950
EcoT14I	CCWWGG	7 cut(s)	121, 275, 700, 822, 930, 1239, 1525
EcoT22I	ATGCAT	1 cut(s)	396
EcoT38I	GRGCYC	4 cut(s)	618, 675, 737, 1365
ErhI	CCWWGG	7 cut(s)	121, 275, 700, 822, 930, 1239, 1525
FaqI	GGGAC	2 cut(s)	58, 921
FauI	CCCGC	2 cut(s)	1061, 1647
Fnu4HI	GCNGC	7 cut(s)	23, 402, 462, 544, 1002, 1455, 1508
FokI	GGATG	6 cut(s)	22, 296, 485, 957, 1415, 1445
FriOI	GRGCYC	4 cut(s)	618, 675, 737, 1365
Fsp4HI	GCNGC	7 cut(s)	23, 402, 462, 544, 1002, 1455, 1508
FspBI	CTAG	4 cut(s)	122, 1005, 1080, 1526
GlaI	GCGC	2 cut(s)	1000, 1520
GluI	GCNGC	7 cut(s)	23, 402, 462, 544, 1002, 1455, 1508
GsaI	CCCAGC	1 cut(s)	136
GsuI	CTGGAG	1 cut(s)	1298
HaeII	RGCGCY	1 cut(s)	1522
HapII	CCGG	6 cut(s)	318, 405, 873, 1301, 1365, 1511
HgaI	GACGC	2 cut(s)	559, 1418
HhaI	GCGC	2 cut(s)	1001, 1521
Hin1I	GRCGYC	2 cut(s)	570, 1410
Hin6I	GCGC	2 cut(s)	999, 1519
HinP1I	GCGC	2 cut(s)	999, 1519
HincII	GTYRAC	2 cut(s)	61, 208
HindII	GTYRAC	2 cut(s)	61, 208
HindIII	AAGCTT	2 cut(s)	716, 1097
HinfI	GANTC	6 cut(s)	271, 761, 852, 1119, 1170, 1492
HpaII	CCGG	6 cut(s)	318, 405, 873, 1301, 1365, 1511
HphI	GGTGA	8 cut(s)	108, 506, 616, 847, 862, 1042, 1212, 1228
Hpy166II	GTNNAC	5 cut(s)	61, 208, 504, 1132, 1374
Hpy188I	TCNGA	3 cut(s)	357, 890, 1504
Hpy188III	TCNNGA	6 cut(s)	467, 676, 724, 1315, 1406, 1573
Hpy8I	GTNNAC	5 cut(s)	61, 208, 504, 1132, 1374
Hpy99I	CGWCG	3 cut(s)	1094, 1355, 1412
HpyAV	CCTTC	4 cut(s)	625, 1174, 1198, 1412
HpyCH4III	ACNGT	3 cut(s)	65, 484, 745
HpyCH4IV	ACGT	6 cut(s)	198, 210, 666, 1128, 1542, 1652
HpyCH4V	TGCA	5 cut(s)	383, 394, 663, 775, 1226
HpyF10VI	GCNNNNNNNGC	8 cut(s)	407, 432, 549, 772, 1001, 1085, 1346, 1516
HpyF3I	CTNAG	5 cut(s)	37, 524, 1017, 1496, 1571
HpySE526I	ACGT	6 cut(s)	198, 210, 666, 1128, 1542, 1652
Hsp92I	GRCGYC	2 cut(s)	570, 1410
HspAI	GCGC	2 cut(s)	999, 1519
Kzo9I	GATC	8 cut(s)	70, 352, 657, 696, 1022, 1210, 1233, 1284
LguI	GCTCTTC	1 cut(s)	1301
LmnI	GCTCC	5 cut(s)	469, 740, 940, 1360, 1571
Lsp1109I	GCAGC	6 cut(s)	9, 413, 448, 1013, 1441, 1519
LweI	GCATC	2 cut(s)	435, 891
MaeI	CTAG	4 cut(s)	122, 1005, 1080, 1526
MaeII	ACGT	6 cut(s)	198, 210, 666, 1128, 1542, 1652
MaeIII	GTNAC	3 cut(s)	97, 494, 802
MalI	GATC	8 cut(s)	72, 354, 659, 698, 1024, 1212, 1235, 1286
MboI	GATC	8 cut(s)	70, 352, 657, 696, 1022, 1210, 1233, 1284
MboII	GAAGA	7 cut(s)	76, 205, 280, 613, 1177, 1318, 1402
MflI	RGATCY	4 cut(s)	70, 657, 696, 1022
MhlI	GDGCHC	6 cut(s)	308, 618, 675, 737, 870, 1365
MlsI	TGGCCA	3 cut(s)	156, 557, 783
MluCI	AATT	6 cut(s)	48, 158, 338, 439, 444, 530
MluNI	TGGCCA	3 cut(s)	156, 557, 783
Mox20I	TGGCCA	3 cut(s)	156, 557, 783
Mph1103I	ATGCAT	1 cut(s)	396
MroXI	GAANNNNTTC	1 cut(s)	1184
MscI	TGGCCA	3 cut(s)	156, 557, 783
MseI	TTAA	4 cut(s)	443, 1044, 1466, 1560
MslI	CAYNNNNRTG	3 cut(s)	248, 1433, 1631
Msp20I	TGGCCA	3 cut(s)	156, 557, 783
MspA1I	CMGCKG	1 cut(s)	543
MspI	CCGG	6 cut(s)	318, 405, 873, 1301, 1365, 1511
MspR9I	CCNGG	7 cut(s)	165, 318, 559, 952, 1301, 1365, 1366
MvaI	CCWGG	3 cut(s)	165, 559, 952
MwoI	GCNNNNNNNGC	8 cut(s)	407, 432, 549, 772, 1001, 1085, 1346, 1516
NciI	CCSGG	4 cut(s)	318, 1301, 1365, 1366
NcoI	CCATGG	2 cut(s)	275, 1239
NdeII	GATC	8 cut(s)	70, 352, 657, 696, 1022, 1210, 1233, 1284
NheI	GCTAGC	1 cut(s)	1079
NmuCI	GTSAC	3 cut(s)	97, 494, 802
NsiI	ATGCAT	1 cut(s)	396
NspI	RCATGY	2 cut(s)	1591, 1630
OliI	CACNNNNGTG	1 cut(s)	248
PaeI	GCATGC	1 cut(s)	1630
PaqCI	CACCTGC	1 cut(s)	1231
PceI	AGGCCT	3 cut(s)	420, 562, 1009
PciSI	GCTCTTC	1 cut(s)	1301
PcsI	WCGNNNNNNNCGW	2 cut(s)	465, 1353
PdmI	GAANNNNTTC	1 cut(s)	1184
PfeI	GAWTC	6 cut(s)	271, 761, 852, 1119, 1170, 1492
PflFI	GACNNNGTC	1 cut(s)	569
PflMI	CCANNNNNTGG	1 cut(s)	1449
PfoI	TCCNGGA	1 cut(s)	163
PkrI	GCNGC	7 cut(s)	24, 403, 463, 545, 1003, 1456, 1509
PmaCI	CACGTG	1 cut(s)	1129
PmlI	CACGTG	1 cut(s)	1129
Ppu21I	YACGTR	3 cut(s)	1129, 1543, 1653
PpuMI	RGGWCCY	1 cut(s)	908
Psp1406I	AACGTT	1 cut(s)	198
Psp5II	RGGWCCY	1 cut(s)	908
Psp6I	CCWGG	3 cut(s)	163, 557, 950
PspCI	CACGTG	1 cut(s)	1129
PspFI	CCCAGC	1 cut(s)	132
PspGI	CCWGG	3 cut(s)	163, 557, 950
PspPI	GGNCC	5 cut(s)	167, 908, 948, 1132, 1374
PspPPI	RGGWCCY	1 cut(s)	908
PstI	CTGCAG	1 cut(s)	1228
PstNI	CAGNNNCTG	1 cut(s)	660
PsuI	RGATCY	4 cut(s)	70, 657, 696, 1022
PsyI	GACNNNGTC	1 cut(s)	569
RsaI	GTAC	5 cut(s)	322, 374, 503, 653, 1583
RsaNI	GTAC	5 cut(s)	321, 373, 502, 652, 1582
RseI	CAYNNNNRTG	3 cut(s)	248, 1433, 1631
SapI	GCTCTTC	1 cut(s)	1301
SaqAI	TTAA	4 cut(s)	443, 1044, 1466, 1560
SatI	GCNGC	7 cut(s)	23, 402, 462, 544, 1002, 1455, 1508
Sau3AI	GATC	8 cut(s)	70, 352, 657, 696, 1022, 1210, 1233, 1284
Sau96I	GGNCC	5 cut(s)	167, 908, 948, 1132, 1374
ScrFI	CCNGG	7 cut(s)	165, 318, 559, 952, 1301, 1365, 1366
SduI	GDGCHC	6 cut(s)	308, 618, 675, 737, 870, 1365
SfaNI	GCATC	2 cut(s)	435, 891
SfcI	CTRYAG	1 cut(s)	1224
SinI	GGWCC	4 cut(s)	167, 908, 1132, 1374
SmaI	CCCGGG	1 cut(s)	1366
SmiMI	CAYNNNNRTG	3 cut(s)	248, 1433, 1631
SmlI	CTYRAG	2 cut(s)	674, 1148
SmoI	CTYRAG	2 cut(s)	674, 1148
SphI	GCATGC	1 cut(s)	1630
Sse9I	AATT	6 cut(s)	48, 158, 338, 439, 444, 530
SseBI	AGGCCT	3 cut(s)	420, 562, 1009
SsiI	CCGC	4 cut(s)	424, 543, 1054, 1640
SspI	AATATT	1 cut(s)	1558
SspMI	CTAG	4 cut(s)	122, 1005, 1080, 1526
StuI	AGGCCT	3 cut(s)	420, 562, 1009
StyD4I	CCNGG	7 cut(s)	163, 316, 557, 950, 1299, 1363, 1364
StyI	CCWWGG	7 cut(s)	121, 275, 700, 822, 930, 1239, 1525
TaaI	ACNGT	3 cut(s)	65, 484, 745
TaiI	ACGT	6 cut(s)	201, 213, 669, 1131, 1545, 1655
TaqI	TCGA	4 cut(s)	723, 1194, 1407, 1473
TasI	AATT	6 cut(s)	48, 158, 338, 439, 444, 530
TatI	WGTACW	3 cut(s)	372, 651, 1581
TauI	GCSGC	1 cut(s)	546
TfiI	GAWTC	6 cut(s)	271, 761, 852, 1119, 1170, 1492
Tru1I	TTAA	4 cut(s)	443, 1044, 1466, 1560
Tru9I	TTAA	4 cut(s)	443, 1044, 1466, 1560
TscAI	CASTG	4 cut(s)	45, 487, 811, 997
TseFI	GTSAC	3 cut(s)	97, 494, 802
TseI	GCWGC	6 cut(s)	22, 401, 461, 1001, 1454, 1507
Tsp45I	GTSAC	3 cut(s)	97, 494, 802
TspDTI	ATGAA	4 cut(s)	358, 644, 728, 1217
TspGWI	ACGGA	5 cut(s)	120, 959, 1131, 1163, 1347
TspMI	CCCGGG	1 cut(s)	1364
TspRI	CASTG	4 cut(s)	45, 487, 811, 997
Tth111I	GACNNNGTC	1 cut(s)	569
Van91I	CCANNNNNTGG	1 cut(s)	1449
VpaK11BI	GGWCC	4 cut(s)	167, 908, 1132, 1374
XagI	CCTNNNNNAGG	1 cut(s)	1619
XapI	RAATTY	2 cut(s)	338, 530
XceI	RCATGY	2 cut(s)	1591, 1630
XcmI	CCANNNNNNNNNTGG	1 cut(s)	937
XmaI	CCCGGG	1 cut(s)	1364
XmaJI	CCTAGG	2 cut(s)	121, 1525
XmnI	GAANNNNTTC	1 cut(s)	1184
XspI	CTAG	4 cut(s)	122, 1005, 1080, 1526
Zsp2I	ATGCAT	1 cut(s)	396

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.