Rorug05G0489500

L-ascorbate oxidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001996
Physical Location & Seq
Reverse (-)
67306631 .. 67308047
1417 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0489500.1

Sequence Viewer

Length: 729 bp
ATGGCAAAGAATAAGTGCGATGGATGTATTAAATTGGAGGAACACATTAAGTTAGCAAGAGAGATAAAGAGACACAAAGAGGAAGTTAATGCTCTTAAGTTTCAAATGTCAGATGAAGCACTTCAACAGATGCCAGATTTTCAAGGCCGGATAGATGTTCTGAAGGATATCGGATGTATAGATGCTGACCTTGTAGTTCAAATAAAAGGCCGTGTCGCATGCGAGATGAATTCTGGGGAGGAGTTGATCTGCACAGAGTGTTTGTTTGAGAACCAACTAGATGACCTGGAACCAGAAGAAGCGGTGGCATTAATGTCGTCCTTCGTTTTTCAGCAGAAGAATACTTCTGAACCTTCACTTACTCCTAAACTTTCTATGGCGAAAGAGAGATTGTACAATACAGCAATAAGACTAGGCAAGCGTCAAGCTTACTTCAAACTTCCAATAAATCCAGAAGAGTACGCCAAAGAGAATCTCAAGTTTGGTCTTGTTGAAGTCGTTTATGAGTGGGCGAAGGGCACACCATTTGCAGATATTTGTGAACTAACAGATGTTCCTGAAGGCATGATAGTGCGGACCATTGTTAGACTTGATGAGACGTGTCGTGAATTCAAAAATGCTGCATCTATTATGGGTAATTCTGCTCTATACAAGAAAATGGAGACAGCTTCAAATGCAATAAAACGTGACATTGTTTTCGCAGCTAGCTTGTATGTAACAGGAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

242

Amino Acids

27.36

Weight (kDa)

5.15

Isoelectric Point (pI)

40.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DSHCT PF08148 64 - 234 7.4e-60 DSHCT (NUC185) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 302, 574
AcsI RAATTY 2 cut(s) 229, 608
AcuI CTGAAG 2 cut(s) 182, 579
AdeI CACNNNGTG 1 cut(s) 258
AfaI GTAC 2 cut(s) 395, 461
AflII CTTAAG 1 cut(s) 95
AflIII ACRYGT 1 cut(s) 599
AgsI TTSAA 8 cut(s) 104, 125, 143, 200, 436, 494, 613, 672
AjiI CACGTC 1 cut(s) 600
AjnI CCWGG 1 cut(s) 285
AluBI AGCT 4 cut(s) 428, 668, 704, 708
AluI AGCT 4 cut(s) 428, 668, 704, 708
Alw26I GTCTC 3 cut(s) 64, 590, 656
AoxI GGCC 2 cut(s) 145, 208
ApeKI GCWGC 2 cut(s) 620, 701
ApoI RAATTY 2 cut(s) 229, 608
ArsI GACNNNNNNTTYG 2 cut(s) 680, 712
AseI ATTAAT 1 cut(s) 311
Asp700I GAANNNNTTC 1 cut(s) 120
AspS9I GGNCC 1 cut(s) 576
AsuNHI GCTAGC 1 cut(s) 704
AvaII GGWCC 1 cut(s) 576
BaeGI GKGCMC 1 cut(s) 521
BbvI GCAGC 2 cut(s) 607, 713
BccI CCATC 1 cut(s) 14
BceAI ACGGC 1 cut(s) 195
BcgI CGANNNNNNTGC 2 cut(s) 297, 331
BciT130I CCWGG 1 cut(s) 287
BcoDI GTCTC 3 cut(s) 64, 590, 656
BfaI CTAG 3 cut(s) 278, 413, 705
BfrI CTTAAG 1 cut(s) 95
BisI GCNGC 2 cut(s) 621, 702
BlsI GCNGC 2 cut(s) 622, 703
Bme1390I CCNGG 1 cut(s) 287
Bme18I GGWCC 1 cut(s) 576
BmgBI CACGTC 1 cut(s) 600
BmgT120I GGNCC 1 cut(s) 576
BmiI GGNNCC 1 cut(s) 291
BmrFI CCNGG 1 cut(s) 287
BmsI GCATC 3 cut(s) 120, 172, 632
BmtI GCTAGC 1 cut(s) 708
BpuEI CTTGAG 1 cut(s) 461
BseBI CCWGG 1 cut(s) 287
BseGI GGATG 2 cut(s) 29, 179
BseRI GAGGAG 1 cut(s) 254
BseSI GKGCMC 1 cut(s) 521
BseXI GCAGC 2 cut(s) 607, 713
BsgI GTGCAG 1 cut(s) 235
BshFI GGCC 2 cut(s) 147, 210
BsiSI CCGG 1 cut(s) 148
BsmAI GTCTC 3 cut(s) 64, 590, 656
BsmBI CGTCTC 1 cut(s) 590
BsnI GGCC 2 cut(s) 147, 210
Bsp1286I GDGCHC 1 cut(s) 521
Bsp1407I TGTACA 1 cut(s) 393
Bsp143I GATC 1 cut(s) 246
BspACI CCGC 2 cut(s) 302, 574
BspANI GGCC 2 cut(s) 147, 210
BspLI GGNNCC 1 cut(s) 291
BspOI GCTAGC 1 cut(s) 708
BspTI CTTAAG 1 cut(s) 95
BsrGI TGTACA 1 cut(s) 393
BssMI GATC 1 cut(s) 246
Bst2UI CCWGG 1 cut(s) 287
Bst6I CTCTTC 1 cut(s) 450
BstAFI CTTAAG 1 cut(s) 95
BstAUI TGTACA 1 cut(s) 393
BstC8I GCNNGC 3 cut(s) 220, 419, 706
BstF5I GGATG 2 cut(s) 29, 179
BstKTI GATC 1 cut(s) 249
BstMAI GTCTC 3 cut(s) 64, 590, 656
BstMBI GATC 1 cut(s) 246
BstMWI GCNNNNNNNGC 1 cut(s) 674
BstNI CCWGG 1 cut(s) 287
BstNSI RCATGY 1 cut(s) 222
BstSCI CCNGG 1 cut(s) 285
BstSLI GKGCMC 1 cut(s) 521
BstV1I GCAGC 2 cut(s) 607, 713
BsuRI GGCC 2 cut(s) 147, 210
BtgZI GCGATG 1 cut(s) 33
BtrI CACGTC 1 cut(s) 600
BtsCI GGATG 2 cut(s) 29, 179
Cac8I GCNNGC 3 cut(s) 220, 419, 706
Cfr13I GGNCC 1 cut(s) 576
CseI GACGC 1 cut(s) 410
Csp6I GTAC 2 cut(s) 394, 460
CviAII CATG 2 cut(s) 219, 565
CviJI RGCY 6 cut(s) 147, 210, 428, 668, 704, 708
CviKI_1 RGCY 6 cut(s) 147, 210, 428, 668, 704, 708
CviQI GTAC 2 cut(s) 394, 460
DpnI GATC 1 cut(s) 248
DpnII GATC 1 cut(s) 246
DraIII CACNNNGTG 1 cut(s) 258
Eam1104I CTCTTC 1 cut(s) 450
EarI CTCTTC 1 cut(s) 450
Eco32I GATATC 1 cut(s) 169
Eco47I GGWCC 1 cut(s) 576
Eco57I CTGAAG 2 cut(s) 182, 579
EcoRI GAATTC 2 cut(s) 229, 608
EcoRII CCWGG 1 cut(s) 285
EcoRV GATATC 1 cut(s) 169
Esp3I CGTCTC 1 cut(s) 590
FaeI CATG 2 cut(s) 222, 568
FaiI YATR 9 cut(s) 179, 220, 377, 504, 566, 632, 649, 714, 727
FatI CATG 2 cut(s) 218, 564
Fnu4HI GCNGC 2 cut(s) 621, 702
FokI GGATG 2 cut(s) 36, 186
Fsp4HI GCNGC 2 cut(s) 621, 702
FspBI CTAG 3 cut(s) 278, 413, 705
GluI GCNGC 2 cut(s) 621, 702
HaeIII GGCC 2 cut(s) 147, 210
HapII CCGG 1 cut(s) 148
HgaI GACGC 1 cut(s) 410
Hin1II CATG 2 cut(s) 222, 568
HindIII AAGCTT 1 cut(s) 426
HinfI GANTC 1 cut(s) 472
HpaII CCGG 1 cut(s) 148
Hpy166II GTNNAC 1 cut(s) 542
Hpy188I TCNGA 4 cut(s) 112, 162, 173, 349
Hpy188III TCNNGA 3 cut(s) 452, 557, 605
Hpy8I GTNNAC 1 cut(s) 542
HpyAV CCTTC 5 cut(s) 157, 331, 363, 508, 554
HpyCH4IV ACGT 2 cut(s) 599, 685
HpyCH4V TGCA 4 cut(s) 252, 530, 623, 677
HpyF10VI GCNNNNNNNGC 1 cut(s) 674
HpySE526I ACGT 2 cut(s) 599, 685
Hsp92II CATG 2 cut(s) 222, 568
Kzo9I GATC 1 cut(s) 246
LpnPI CCDG 9 cut(s) 147, 161, 219, 272, 299, 306, 465, 570, 705
Lsp1109I GCAGC 2 cut(s) 607, 713
LweI GCATC 3 cut(s) 120, 172, 632
MaeI CTAG 3 cut(s) 278, 413, 705
MaeII ACGT 2 cut(s) 599, 685
MaeIII GTNAC 2 cut(s) 686, 715
MalI GATC 1 cut(s) 248
MboI GATC 1 cut(s) 246
MboII GAAGA 3 cut(s) 308, 349, 467
MhlI GDGCHC 1 cut(s) 521
MluCI AATT 4 cut(s) 32, 229, 608, 637
MnlI CCTC 3 cut(s) 31, 73, 232
MroXI GAANNNNTTC 1 cut(s) 120
MseI TTAA 5 cut(s) 30, 48, 87, 96, 311
MslI CAYNNNNRTG 1 cut(s) 569
MspCI CTTAAG 1 cut(s) 95
MspI CCGG 1 cut(s) 148
MspR9I CCNGG 1 cut(s) 287
MvaI CCWGG 1 cut(s) 287
MwoI GCNNNNNNNGC 1 cut(s) 674
NdeII GATC 1 cut(s) 246
NheI GCTAGC 1 cut(s) 704
NlaIII CATG 2 cut(s) 222, 568
NlaIV GGNNCC 1 cut(s) 291
NmuCI GTSAC 1 cut(s) 686
NspI RCATGY 1 cut(s) 222
PaeI GCATGC 1 cut(s) 222
PdmI GAANNNNTTC 1 cut(s) 120
PfeI GAWTC 1 cut(s) 472
PkrI GCNGC 2 cut(s) 622, 703
PshBI ATTAAT 1 cut(s) 311
Psp6I CCWGG 1 cut(s) 285
PspGI CCWGG 1 cut(s) 285
PspN4I GGNNCC 1 cut(s) 291
PspPI GGNCC 1 cut(s) 576
RsaI GTAC 2 cut(s) 395, 461
RsaNI GTAC 2 cut(s) 394, 460
RseI CAYNNNNRTG 1 cut(s) 569
SaqAI TTAA 5 cut(s) 30, 48, 87, 96, 311
SatI GCNGC 2 cut(s) 621, 702
Sau3AI GATC 1 cut(s) 246
Sau96I GGNCC 1 cut(s) 576
ScrFI CCNGG 1 cut(s) 287
SduI GDGCHC 1 cut(s) 521
SetI ASST 9 cut(s) 192, 288, 355, 430, 602, 670, 688, 706, 710
SfaNI GCATC 3 cut(s) 120, 172, 632
SinI GGWCC 1 cut(s) 576
SmiMI CAYNNNNRTG 1 cut(s) 569
SmlI CTYRAG 2 cut(s) 95, 476
SmoI CTYRAG 2 cut(s) 95, 476
SphI GCATGC 1 cut(s) 222
Sse9I AATT 4 cut(s) 32, 229, 608, 637
SsiI CCGC 2 cut(s) 302, 574
SspMI CTAG 3 cut(s) 278, 413, 705
StyD4I CCNGG 1 cut(s) 285
TaiI ACGT 2 cut(s) 602, 688
TasI AATT 4 cut(s) 32, 229, 608, 637
TatI WGTACW 1 cut(s) 393
TfiI GAWTC 1 cut(s) 472
Tru1I TTAA 5 cut(s) 30, 48, 87, 96, 311
Tru9I TTAA 5 cut(s) 30, 48, 87, 96, 311
TseFI GTSAC 1 cut(s) 686
TseI GCWGC 2 cut(s) 620, 701
Tsp45I GTSAC 1 cut(s) 686
TspDTI ATGAA 2 cut(s) 129, 242
Vha464I CTTAAG 1 cut(s) 95
VpaK11BI GGWCC 1 cut(s) 576
VspI ATTAAT 1 cut(s) 311
XapI RAATTY 2 cut(s) 229, 608
XceI RCATGY 1 cut(s) 222
XmnI GAANNNNTTC 1 cut(s) 120
XspI CTAG 3 cut(s) 278, 413, 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.