RLG00000001981

L-ascorbate oxidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001996
Physical Location & Seq
Reverse (-)
23889477 .. 23890187
711 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001981

Sequence Viewer

Length: 465 bp
ATGGTGAAGATCCTTACCTTTTCTTCACATGGAATAGACAAGATCGCCTTAGCACCTAATGTTGTCAACCAAACCTACCGGGACTTTGTGGAGATTATCTTTGAGAACCACGAGAGGAGCATTCAGTCATGGCATCTGGATGGATTCGCTTTCTTTCCCGCTGCCATTGAGTTTGGGAGGTGGGCACTAGAGAAGAGAAGGAACTACAACCTTCTGGACGCCATAAGTCGACACACTGTCCACGTGTATCCTGGCTCATGGGCCTCCATCATGTTGACATTCGACAACGCTGAAATGTGGAACTTGAGGTCAGCACAGCCGGAGAGGGCGTACCTAGAACAACAGCTCTACCTGAGTGTTGAAAATGTCGCACGCTCTCTCAGGGACGAGTACATGATGCCAGACCAGACACCTCTCTGTGGCATCGTCAAGGGTCTCCCTCAACCCCCTCCATACGGCCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

155

Amino Acids

17.81

Weight (kDa)

5.92

Isoelectric Point (pI)

58.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_2 PF07731 18 - 122 1.2e-22 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 229
AciI CCGC 1 cut(s) 159
AclWI GGATC 1 cut(s) 4
AcvI CACGTG 1 cut(s) 244
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 2 cut(s) 332, 392
AfiI CCNNNNNNNGG 2 cut(s) 419, 455
AflIII ACRYGT 1 cut(s) 243
AgsI TTSAA 1 cut(s) 362
AhdI GACNNNNNGTC 1 cut(s) 236
AjnI CCWGG 1 cut(s) 250
AluBI AGCT 1 cut(s) 346
AluI AGCT 1 cut(s) 346
Alw26I GTCTC 1 cut(s) 440
AlwI GGATC 1 cut(s) 4
AoxI GGCC 2 cut(s) 261, 457
ApeKI GCWGC 1 cut(s) 161
AspS9I GGNCC 2 cut(s) 261, 458
AsuC2I CCSGG 1 cut(s) 80
AsuHPI GGTGA 1 cut(s) 16
BaeGI GKGCMC 1 cut(s) 187
BauI CACGAG 1 cut(s) 110
BbrPI CACGTG 1 cut(s) 244
BbvI GCAGC 1 cut(s) 148
BccI CCATC 2 cut(s) 134, 275
BciT130I CCWGG 1 cut(s) 252
BciVI GTATCC 1 cut(s) 258
BcnI CCSGG 1 cut(s) 80
BcoDI GTCTC 1 cut(s) 440
BfaI CTAG 2 cut(s) 188, 335
BfuI GTATCC 1 cut(s) 258
BisI GCNGC 1 cut(s) 162
BlsI GCNGC 1 cut(s) 163
Bme1390I CCNGG 2 cut(s) 80, 252
BmeRI GACNNNNNGTC 1 cut(s) 236
BmgT120I GGNCC 2 cut(s) 261, 458
BmrFI CCNGG 2 cut(s) 80, 252
BmsI GCATC 3 cut(s) 142, 387, 432
Bpu10I CCTNAGC 1 cut(s) 49
BpuEI CTTGAG 1 cut(s) 325
BpuMI CCSGG 1 cut(s) 80
BsaAI YACGTR 1 cut(s) 244
BsaHI GRCGYC 1 cut(s) 219
BsaI GGTCTC 1 cut(s) 440
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 2 cut(s) 419, 455
BseBI CCWGG 1 cut(s) 252
BseGI GGATG 1 cut(s) 145
BseLI CCNNNNNNNGG 2 cut(s) 419, 455
BseMII CTCAG 2 cut(s) 344, 394
BseRI GAGGAG 1 cut(s) 130
BseSI GKGCMC 1 cut(s) 187
BseXI GCAGC 1 cut(s) 148
BshFI GGCC 2 cut(s) 263, 459
BsiSI CCGG 2 cut(s) 79, 320
BslFI GGGAC 2 cut(s) 95, 398
BslI CCNNNNNNNGG 2 cut(s) 419, 455
BsmAI GTCTC 1 cut(s) 440
BsmFI GGGAC 2 cut(s) 95, 398
BsmI GAATGC 1 cut(s) 120
BsnI GGCC 2 cut(s) 263, 459
Bso31I GGTCTC 1 cut(s) 440
Bsp1286I GDGCHC 1 cut(s) 187
Bsp143I GATC 2 cut(s) 9, 42
BspACI CCGC 1 cut(s) 159
BspANI GGCC 2 cut(s) 263, 459
BspCNI CTCAG 2 cut(s) 345, 393
BspPI GGATC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 440
BssMI GATC 2 cut(s) 9, 42
BssNI GRCGYC 1 cut(s) 219
BssSI CACGAG 1 cut(s) 110
Bst2BI CACGAG 1 cut(s) 110
Bst2UI CCWGG 1 cut(s) 252
Bst4CI ACNGT 1 cut(s) 238
Bst6I CTCTTC 1 cut(s) 188
BstACI GRCGYC 1 cut(s) 219
BstBAI YACGTR 1 cut(s) 244
BstC8I GCNNGC 1 cut(s) 373
BstDEI CTNAG 3 cut(s) 49, 353, 380
BstF5I GGATG 1 cut(s) 145
BstKTI GATC 2 cut(s) 12, 45
BstMAI GTCTC 1 cut(s) 440
BstMBI GATC 2 cut(s) 9, 42
BstNI CCWGG 1 cut(s) 252
BstSCI CCNGG 2 cut(s) 78, 250
BstSLI GKGCMC 1 cut(s) 187
BstV1I GCAGC 1 cut(s) 148
BstX2I RGATCY 1 cut(s) 9
BstYI RGATCY 1 cut(s) 9
BsuI GTATCC 1 cut(s) 258
BsuRI GGCC 2 cut(s) 263, 459
BtsCI GGATG 1 cut(s) 145
BtsIMutI CAGTG 1 cut(s) 234
Cac8I GCNNGC 1 cut(s) 373
Cfr13I GGNCC 2 cut(s) 261, 458
CseI GACGC 1 cut(s) 227
Csp6I GTAC 2 cut(s) 331, 391
CviAII CATG 5 cut(s) 29, 129, 258, 271, 394
CviJI RGCY 5 cut(s) 255, 263, 319, 346, 459
CviKI_1 RGCY 5 cut(s) 255, 263, 319, 346, 459
CviQI GTAC 2 cut(s) 331, 391
DdeI CTNAG 3 cut(s) 49, 353, 380
DpnI GATC 2 cut(s) 11, 44
DpnII GATC 2 cut(s) 9, 42
DriI GACNNNNNGTC 1 cut(s) 236
Eam1104I CTCTTC 1 cut(s) 188
Eam1105I GACNNNNNGTC 1 cut(s) 236
EarI CTCTTC 1 cut(s) 188
Eco31I GGTCTC 1 cut(s) 440
Eco72I CACGTG 1 cut(s) 244
EcoRII CCWGG 1 cut(s) 250
FaeI CATG 5 cut(s) 32, 132, 261, 274, 397
FaiI YATR 7 cut(s) 30, 130, 224, 259, 272, 395, 454
FalI AAGNNNNNCTT 2 cut(s) 32, 64
FaqI GGGAC 2 cut(s) 95, 398
FatI CATG 5 cut(s) 28, 128, 257, 270, 393
FauI CCCGC 1 cut(s) 166
FblI GTMKAC 1 cut(s) 229
Fnu4HI GCNGC 1 cut(s) 162
FokI GGATG 1 cut(s) 152
Fsp4HI GCNGC 1 cut(s) 162
FspBI CTAG 2 cut(s) 188, 335
GluI GCNGC 1 cut(s) 162
HaeIII GGCC 2 cut(s) 263, 459
HapII CCGG 2 cut(s) 79, 320
HgaI GACGC 1 cut(s) 227
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 5 cut(s) 32, 132, 261, 274, 397
HincII GTYRAC 3 cut(s) 67, 230, 276
HindII GTYRAC 3 cut(s) 67, 230, 276
HinfI GANTC 1 cut(s) 144
HpaII CCGG 2 cut(s) 79, 320
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 4 cut(s) 67, 230, 241, 276
Hpy188III TCNNGA 2 cut(s) 137, 215
Hpy8I GTNNAC 4 cut(s) 67, 230, 241, 276
HpyAV CCTTC 2 cut(s) 192, 221
HpyCH4III ACNGT 1 cut(s) 238
HpyCH4IV ACGT 1 cut(s) 243
HpyF3I CTNAG 3 cut(s) 49, 353, 380
HpySE526I ACGT 1 cut(s) 243
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 5 cut(s) 32, 132, 261, 274, 397
Kzo9I GATC 2 cut(s) 9, 42
LmnI GCTCC 1 cut(s) 117
Lsp1109I GCAGC 1 cut(s) 148
LweI GCATC 3 cut(s) 142, 387, 432
MaeI CTAG 2 cut(s) 188, 335
MaeII ACGT 1 cut(s) 243
MalI GATC 2 cut(s) 11, 44
MboI GATC 2 cut(s) 9, 42
MboII GAAGA 3 cut(s) 15, 19, 205
MflI RGATCY 1 cut(s) 9
MhlI GDGCHC 1 cut(s) 187
MnlI CCTC 8 cut(s) 108, 171, 274, 300, 318, 423, 450, 459
MseI TTAA 1 cut(s) 463
MslI CAYNNNNRTG 1 cut(s) 138
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 2 cut(s) 79, 320
MspR9I CCNGG 2 cut(s) 80, 252
Mva1269I GAATGC 1 cut(s) 120
MvaI CCWGG 1 cut(s) 252
NciI CCSGG 1 cut(s) 80
NdeII GATC 2 cut(s) 9, 42
NlaIII CATG 5 cut(s) 32, 132, 261, 274, 397
PctI GAATGC 1 cut(s) 120
PfeI GAWTC 1 cut(s) 144
PkrI GCNGC 1 cut(s) 163
PmaCI CACGTG 1 cut(s) 244
PmlI CACGTG 1 cut(s) 244
Ppu21I YACGTR 1 cut(s) 244
Psp6I CCWGG 1 cut(s) 250
PspCI CACGTG 1 cut(s) 244
PspGI CCWGG 1 cut(s) 250
PspPI GGNCC 2 cut(s) 261, 458
PsuI RGATCY 1 cut(s) 9
RsaI GTAC 2 cut(s) 332, 392
RsaNI GTAC 2 cut(s) 331, 391
RseI CAYNNNNRTG 1 cut(s) 138
SalI GTCGAC 1 cut(s) 228
SaqAI TTAA 1 cut(s) 463
SatI GCNGC 1 cut(s) 162
Sau3AI GATC 2 cut(s) 9, 42
Sau96I GGNCC 2 cut(s) 261, 458
ScrFI CCNGG 2 cut(s) 80, 252
SduI GDGCHC 1 cut(s) 187
SfaNI GCATC 3 cut(s) 142, 387, 432
SmiMI CAYNNNNRTG 1 cut(s) 138
SmlI CTYRAG 1 cut(s) 304
SmoI CTYRAG 1 cut(s) 304
SsiI CCGC 1 cut(s) 159
SspMI CTAG 2 cut(s) 188, 335
StyD4I CCNGG 2 cut(s) 78, 250
TaaI ACNGT 1 cut(s) 238
TaiI ACGT 1 cut(s) 246
TaqI TCGA 2 cut(s) 229, 282
TatI WGTACW 1 cut(s) 390
TfiI GAWTC 1 cut(s) 144
Tru1I TTAA 1 cut(s) 463
Tru9I TTAA 1 cut(s) 463
TscAI CASTG 1 cut(s) 241
TseI GCWGC 1 cut(s) 161
TspRI CASTG 1 cut(s) 241
XcmI CCANNNNNNNNNTGG 1 cut(s) 248
XmiI GTMKAC 1 cut(s) 229
XspI CTAG 2 cut(s) 188, 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.