MD03G1001400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002354
Physical Location & Seq
Reverse (-)
104478 .. 108459
3982 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1001400.v1.1.491

Sequence Viewer

Length: 774 bp
ATGAGTATAAGAATCACTCTCTTAGAACCAAAGCGAGCTTTCATTTTTTGGATGAGGTTTCGTTATTCACATTGCAATACAGAGAAAAGCATTTGCCGTTATATTCCTGCAAACAGACATGCAGATCCAGCTCTTGTGGACTTCCTCAAAACCTCTTTCTCAGGATCCAATTTCTCCATGGCCTTCCCTTTTGTTGCTGCACCGGAGGAGGATTCTATGGAAAATTCTTTGGTGTTAGAGATTTCAGAGACTTGTGGGCAGGATTTGGGATTCAGTAATGTTACTTTATTTGGGTCATGCTCTATTGAGGGTGAAAATTTTCAAAAGCTCTCAAATGTGCAATCATTCCATGATTATCTTGTTTCAGGGGTGGAGAAGAGATCCAAAGGGGAAGTAGATTTGGTTGTGTTCTGCCATAAACAATCTGAATCTTCCAAAGAACTTGATCAAACACATTCTGAGGGTAAAGTTTTTTCTGACCTTGTTAATTCCATGGATCAATCTGGGGCAAAGTATGGAGTTCTATATGTTTCAGATCCCAGTAAGTCGATCCAATATCCTTCTCATCGAGAATTAGAAAGGTTTCTTGCTGAAAGTGGATCAGGAAATGCATCAGCCAATTCGACGGCCTGTGATGAAGTTTGTCAGATAAAATCATCTCTTCTAGAGGGACTTCTAGTTGGAATCATTTTGCTTATAATCTTAATATCGGGCATTTGCTGTATGATGGGGATTGACACTCCGACGCGATTTGAGATGCCCCAAGAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

258

Amino Acids

28.51

Weight (kDa)

5.02

Isoelectric Point (pI)

45.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ac45-VOA1_TM PF20520 221 - 252 5.1e-09 V0 complex accessory subunit Ac45/VOA1 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 698
AccII CGCG 1 cut(s) 748
AclWI GGATC 8 cut(s) 119, 159, 172, 375, 504, 530, 544, 607
AcsI RAATTY 2 cut(s) 223, 316
AgsI TTSAA 1 cut(s) 323
AluBI AGCT 3 cut(s) 38, 131, 328
AluI AGCT 3 cut(s) 38, 131, 328
Alw26I GTCTC 1 cut(s) 242
AlwI GGATC 8 cut(s) 119, 159, 172, 375, 504, 530, 544, 607
AoxI GGCC 2 cut(s) 180, 627
ApeKI GCWGC 1 cut(s) 197
ApoI RAATTY 2 cut(s) 223, 316
Asp700I GAANNNNTTC 2 cut(s) 318, 582
AsuHPI GGTGA 1 cut(s) 323
BamHI GGATCC 1 cut(s) 164
BbvI GCAGC 1 cut(s) 184
BccI CCATC 1 cut(s) 721
BceAI ACGGC 2 cut(s) 81, 642
BclI TGATCA 1 cut(s) 445
BcoDI GTCTC 1 cut(s) 242
BfaI CTAG 2 cut(s) 665, 677
BisI GCNGC 1 cut(s) 198
BlsI GCNGC 1 cut(s) 199
BmiI GGNNCC 1 cut(s) 166
BmrI ACTGGG 1 cut(s) 534
BmsI GCATC 2 cut(s) 620, 747
BmuI ACTGGG 1 cut(s) 534
BsaJI CCNNGG 2 cut(s) 177, 492
BsaWI WCCGGW 1 cut(s) 202
Bse1I ACTGG 1 cut(s) 540
Bse3DI GCAATG 1 cut(s) 70
BseDI CCNNGG 2 cut(s) 177, 492
BseGI GGATG 1 cut(s) 57
BseMI GCAATG 1 cut(s) 70
BseMII CTCAG 2 cut(s) 174, 450
BseNI ACTGG 1 cut(s) 540
BseRI GAGGAG 1 cut(s) 221
BseXI GCAGC 1 cut(s) 184
BsgI GTGCAG 1 cut(s) 183
Bsh1236I CGCG 1 cut(s) 748
BshFI GGCC 2 cut(s) 182, 629
BsiSI CCGG 1 cut(s) 203
BslFI GGGAC 1 cut(s) 684
BsmAI GTCTC 1 cut(s) 242
BsmFI GGGAC 1 cut(s) 684
BsnI GGCC 2 cut(s) 182, 629
Bsp143I GATC 8 cut(s) 124, 164, 380, 445, 496, 535, 549, 599
Bsp19I CCATGG 2 cut(s) 177, 492
BspANI GGCC 2 cut(s) 182, 629
BspCNI CTCAG 2 cut(s) 173, 451
BspFNI CGCG 1 cut(s) 748
BspLI GGNNCC 1 cut(s) 166
BspPI GGATC 8 cut(s) 119, 159, 172, 375, 504, 530, 544, 607
BsrDI GCAATG 1 cut(s) 70
BsrI ACTGG 1 cut(s) 540
BssECI CCNNGG 2 cut(s) 177, 492
BssMI GATC 8 cut(s) 124, 164, 380, 445, 496, 535, 549, 599
BssT1I CCWWGG 2 cut(s) 177, 492
Bst6I CTCTTC 2 cut(s) 371, 666
BstC8I GCNNGC 1 cut(s) 36
BstDEI CTNAG 3 cut(s) 22, 160, 459
BstDSI CCRYGG 2 cut(s) 177, 492
BstF5I GGATG 1 cut(s) 57
BstFNI CGCG 1 cut(s) 748
BstKTI GATC 8 cut(s) 127, 167, 383, 448, 499, 538, 552, 602
BstMAI GTCTC 1 cut(s) 242
BstMBI GATC 8 cut(s) 124, 164, 380, 445, 496, 535, 549, 599
BstMWI GCNNNNNNNGC 1 cut(s) 128
BstNSI RCATGY 1 cut(s) 122
BstUI CGCG 1 cut(s) 748
BstV1I GCAGC 1 cut(s) 184
BstX2I RGATCY 4 cut(s) 124, 164, 380, 535
BstYI RGATCY 4 cut(s) 124, 164, 380, 535
BsuRI GGCC 2 cut(s) 182, 629
BtgI CCRYGG 2 cut(s) 177, 492
BtsCI GGATG 1 cut(s) 57
Cac8I GCNNGC 1 cut(s) 36
CseI GACGC 1 cut(s) 754
CviAII CATG 5 cut(s) 119, 178, 297, 350, 493
CviJI RGCY 6 cut(s) 38, 131, 182, 328, 617, 629
CviKI_1 RGCY 6 cut(s) 38, 131, 182, 328, 617, 629
DdeI CTNAG 3 cut(s) 22, 160, 459
DpnI GATC 8 cut(s) 126, 166, 382, 447, 498, 537, 551, 601
DpnII GATC 8 cut(s) 124, 164, 380, 445, 496, 535, 549, 599
Eam1104I CTCTTC 2 cut(s) 371, 666
EarI CTCTTC 2 cut(s) 371, 666
Eco130I CCWWGG 2 cut(s) 177, 492
EcoT14I CCWWGG 2 cut(s) 177, 492
EcoT22I ATGCAT 1 cut(s) 613
ErhI CCWWGG 2 cut(s) 177, 492
FaeI CATG 5 cut(s) 122, 181, 300, 353, 496
FaqI GGGAC 1 cut(s) 684
FatI CATG 5 cut(s) 118, 177, 296, 349, 492
FbaI TGATCA 1 cut(s) 445
Fnu4HI GCNGC 1 cut(s) 198
FokI GGATG 1 cut(s) 64
Fsp4HI GCNGC 1 cut(s) 198
FspBI CTAG 2 cut(s) 665, 677
GluI GCNGC 1 cut(s) 198
HaeIII GGCC 2 cut(s) 182, 629
HapII CCGG 1 cut(s) 203
HgaI GACGC 1 cut(s) 754
Hin1II CATG 5 cut(s) 122, 181, 300, 353, 496
HinfI GANTC 6 cut(s) 12, 212, 270, 428, 684, 767
HpaII CCGG 1 cut(s) 203
HphI GGTGA 1 cut(s) 323
Hpy166II GTNNAC 1 cut(s) 139
Hpy188I TCNGA 7 cut(s) 247, 427, 460, 478, 535, 648, 744
Hpy188III TCNNGA 5 cut(s) 162, 569, 603, 665, 771
Hpy8I GTNNAC 1 cut(s) 139
Hpy99I CGWCG 2 cut(s) 628, 748
HpyAV CCTTC 2 cut(s) 193, 570
HpyCH4V TGCA 6 cut(s) 75, 110, 122, 200, 340, 611
HpyF10VI GCNNNNNNNGC 1 cut(s) 128
HpyF3I CTNAG 3 cut(s) 22, 160, 459
Hsp92II CATG 5 cut(s) 122, 181, 300, 353, 496
Ksp22I TGATCA 1 cut(s) 445
Kzo9I GATC 8 cut(s) 124, 164, 380, 445, 496, 535, 549, 599
Lsp1109I GCAGC 1 cut(s) 184
LweI GCATC 2 cut(s) 620, 747
MaeI CTAG 2 cut(s) 665, 677
MaeIII GTNAC 1 cut(s) 280
MalI GATC 8 cut(s) 126, 166, 382, 447, 498, 537, 551, 601
MboI GATC 8 cut(s) 124, 164, 380, 445, 496, 535, 549, 599
MboII GAAGA 3 cut(s) 388, 423, 653
MflI RGATCY 4 cut(s) 124, 164, 380, 535
MluCI AATT 6 cut(s) 169, 223, 316, 487, 572, 619
MmeI TCCRAC 2 cut(s) 661, 767
MnlI CCTC 8 cut(s) 48, 155, 163, 199, 202, 301, 454, 661
Mph1103I ATGCAT 1 cut(s) 613
MroXI GAANNNNTTC 2 cut(s) 318, 582
MseI TTAA 2 cut(s) 486, 704
MspI CCGG 1 cut(s) 203
MvnI CGCG 1 cut(s) 748
MwoI GCNNNNNNNGC 1 cut(s) 128
NcoI CCATGG 2 cut(s) 177, 492
NdeII GATC 8 cut(s) 124, 164, 380, 445, 496, 535, 549, 599
NlaIII CATG 5 cut(s) 122, 181, 300, 353, 496
NlaIV GGNNCC 1 cut(s) 166
NsiI ATGCAT 1 cut(s) 613
NspI RCATGY 1 cut(s) 122
PdmI GAANNNNTTC 2 cut(s) 318, 582
PfeI GAWTC 5 cut(s) 12, 212, 270, 428, 684
PkrI GCNGC 1 cut(s) 199
PsiI TTATAA 1 cut(s) 698
PspN4I GGNNCC 1 cut(s) 166
PsuI RGATCY 4 cut(s) 124, 164, 380, 535
SaqAI TTAA 2 cut(s) 486, 704
SatI GCNGC 1 cut(s) 198
Sau3AI GATC 8 cut(s) 124, 164, 380, 445, 496, 535, 549, 599
SetI ASST 7 cut(s) 40, 59, 133, 155, 330, 483, 584
SfaNI GCATC 2 cut(s) 620, 747
Sse9I AATT 6 cut(s) 169, 223, 316, 487, 572, 619
SspMI CTAG 2 cut(s) 665, 677
StyI CCWWGG 2 cut(s) 177, 492
TaqI TCGA 3 cut(s) 548, 568, 623
TasI AATT 6 cut(s) 169, 223, 316, 487, 572, 619
TfiI GAWTC 5 cut(s) 12, 212, 270, 428, 684
Tru1I TTAA 2 cut(s) 486, 704
Tru9I TTAA 2 cut(s) 486, 704
TseI GCWGC 1 cut(s) 197
TspDTI ATGAA 2 cut(s) 31, 651
XapI RAATTY 2 cut(s) 223, 316
XbaI TCTAGA 1 cut(s) 664
XceI RCATGY 1 cut(s) 122
XcmI CCANNNNNNNNNTGG 1 cut(s) 175
XmnI GAANNNNTTC 2 cut(s) 318, 582
XspI CTAG 2 cut(s) 665, 677
Zsp2I ATGCAT 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.