MD11G1000600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002354
Physical Location & Seq
Reverse (-)
94504 .. 97659
3156 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1000600.v1.1.491

Sequence Viewer

Length: 951 bp
ATGAAGGCAGCTCAGCCTCTGGTGCTAGTTTTGGTGGGTTTGCTTGCGCTTGCAGGAGCACCTTCCACTTCAGCTTCCACAGTGCCAGCTTTCCTCTGGTCCCCTCATTACCATATAGTCAAGGCAGCCGTAAATTATCAGACAATATCCCCCAAGGATTTAGCAAAGTCTCTTCTTACCGAAGGTGGCTGGTCCAACGTACTGTGCTCTGCAAACAACGTTCATCAGCCTCTGGAGCTGGCTCTTGTCTTTGTGGGTCAGGAGTTACAATCTTCAGATATTCCTGCAAACAGACATGCAGATCCAGCTCTTGTGGACTTGCTGAAAACCTCTTTCTCAGGATCCAATTTTTCCATGGCCTTCCCTTATGTTGCTGCACCGGAGGATGATTCTATGGAAAGTTCTTTGGTGTCAGAGATTTCAGAGACTTGTGGGCAGGATTTAGGACTCAGTAATGTTGCTTTATTGGGGTCATGCTCGATTGAGGGTGAAAATGTTCAAAAGCTCACAAATGTGCAATCATTCCATGATTACCTTGTTTCAAGGATGGAGAAGAGATCCAATGGGGAAGTAGATTTGGTTGTGTTCTGCCATAAAGGATCTGAATCTTCCAAAGAATTTGACCAACCACATTCTGAGGGTAAAGTTTTTTCTGAGCTTGTTAATTCCTTGGATCAATCTGGGGCAAAGTATGGAGTTCTATATGTTTCAGATCCCAGTAAGTCGATCCAATATCCTTCTCATCGAGAATTAGAAAGATTTCTGGCCGAAAGTGGTTCAGGAAATGCATCAGCCAATTCTACGGCCTGTGATGAAGTTTGTCTATTAAAATCATCTCTTCTAGAGGGACTTCTAGTTGGAATTGTTTTGCTTATAATCTTAATATCGGGCATTTGCTGTATGATGGGGATTGACACTCCAACGAGATTTGAGACGCCCCAGGAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

317

Amino Acids

33.8

Weight (kDa)

4.77

Isoelectric Point (pI)

48.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ac45-VOA1_TM PF20520 280 - 311 3.4e-09 V0 complex accessory subunit Ac45/VOA1 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 875
AclI AACGTT 1 cut(s) 219
AclWI GGATC 8 cut(s) 296, 336, 349, 552, 607, 681, 707, 721
AcoI YGGCCR 1 cut(s) 765
AcsI RAATTY 1 cut(s) 617
AcuI CTGAAG 2 cut(s) 54, 258
AcyI GRCGYC 1 cut(s) 935
AfaI GTAC 1 cut(s) 201
AgsI TTSAA 2 cut(s) 500, 543
AjnI CCWGG 1 cut(s) 939
AleI CACNNNNGTG 1 cut(s) 512
AluBI AGCT 7 cut(s) 11, 74, 89, 238, 308, 505, 658
AluI AGCT 7 cut(s) 11, 74, 89, 238, 308, 505, 658
Alw21I GWGCWC 2 cut(s) 61, 209
Alw26I GTCTC 3 cut(s) 174, 419, 926
AlwI GGATC 8 cut(s) 296, 336, 349, 552, 607, 681, 707, 721
AlwNI CAGNNNCTG 2 cut(s) 19, 232
AoxI GGCC 3 cut(s) 357, 765, 804
ApeKI GCWGC 3 cut(s) 8, 125, 374
ApoI RAATTY 1 cut(s) 617
Asp700I GAANNNNTTC 2 cut(s) 495, 759
AspLEI GCGC 1 cut(s) 49
AspS9I GGNCC 2 cut(s) 99, 192
AsuHPI GGTGA 1 cut(s) 500
AvaII GGWCC 2 cut(s) 99, 192
BamHI GGATCC 1 cut(s) 341
Bbv12I GWGCWC 2 cut(s) 61, 209
BbvI GCAGC 3 cut(s) 20, 137, 361
BccI CCATC 2 cut(s) 541, 898
BceAI ACGGC 2 cut(s) 113, 819
BciT130I CCWGG 1 cut(s) 941
BcoDI GTCTC 3 cut(s) 174, 419, 926
BfaI CTAG 3 cut(s) 26, 842, 854
BisI GCNGC 3 cut(s) 9, 126, 375
BlpI GCTNAGC 1 cut(s) 12
BlsI GCNGC 3 cut(s) 10, 127, 376
Bme1390I CCNGG 1 cut(s) 941
Bme18I GGWCC 2 cut(s) 99, 192
BmgT120I GGNCC 2 cut(s) 99, 192
BmiI GGNNCC 2 cut(s) 101, 343
BmrFI CCNGG 1 cut(s) 941
BmrI ACTGGG 1 cut(s) 711
BmsI GCATC 1 cut(s) 797
BmuI ACTGGG 1 cut(s) 711
BpmI CTGGAG 1 cut(s) 254
Bpu1102I GCTNAGC 1 cut(s) 12
BsaBI GATNNNNATC 1 cut(s) 604
BsaHI GRCGYC 1 cut(s) 935
BsaJI CCNNGG 4 cut(s) 153, 354, 669, 939
BsaWI WCCGGW 1 cut(s) 379
Bse1I ACTGG 1 cut(s) 717
Bse8I GATNNNNATC 1 cut(s) 604
BseBI CCWGG 1 cut(s) 941
BseDI CCNNGG 4 cut(s) 153, 354, 669, 939
BseGI GGATG 2 cut(s) 391, 552
BseJI GATNNNNATC 1 cut(s) 604
BseMII CTCAG 5 cut(s) 26, 351, 463, 627, 645
BseNI ACTGG 1 cut(s) 717
BseXI GCAGC 3 cut(s) 20, 137, 361
BsgI GTGCAG 1 cut(s) 360
BshFI GGCC 3 cut(s) 359, 767, 806
BsiHKAI GWGCWC 2 cut(s) 61, 209
BsiSI CCGG 1 cut(s) 380
BslFI GGGAC 2 cut(s) 85, 861
BsmAI GTCTC 3 cut(s) 174, 419, 926
BsmBI CGTCTC 1 cut(s) 926
BsmFI GGGAC 2 cut(s) 85, 861
BsnI GGCC 3 cut(s) 359, 767, 806
Bsp1286I GDGCHC 2 cut(s) 61, 209
Bsp143I GATC 7 cut(s) 301, 341, 557, 599, 673, 712, 726
Bsp1720I GCTNAGC 1 cut(s) 12
Bsp19I CCATGG 1 cut(s) 354
BspANI GGCC 3 cut(s) 359, 767, 806
BspCNI CTCAG 5 cut(s) 25, 350, 462, 628, 646
BspLI GGNNCC 2 cut(s) 101, 343
BspPI GGATC 8 cut(s) 296, 336, 349, 552, 607, 681, 707, 721
BsrI ACTGG 1 cut(s) 717
BssECI CCNNGG 4 cut(s) 153, 354, 669, 939
BssMI GATC 7 cut(s) 301, 341, 557, 599, 673, 712, 726
BssNI GRCGYC 1 cut(s) 935
BssT1I CCWWGG 3 cut(s) 153, 354, 669
Bst2UI CCWGG 1 cut(s) 941
Bst4CI ACNGT 2 cut(s) 82, 204
Bst6I CTCTTC 3 cut(s) 177, 548, 843
BstACI GRCGYC 1 cut(s) 935
BstC8I GCNNGC 4 cut(s) 45, 51, 87, 240
BstDEI CTNAG 5 cut(s) 12, 337, 449, 636, 654
BstDSI CCRYGG 1 cut(s) 354
BstF5I GGATG 2 cut(s) 391, 552
BstHHI GCGC 1 cut(s) 49
BstKTI GATC 7 cut(s) 304, 344, 560, 602, 676, 715, 729
BstMAI GTCTC 3 cut(s) 174, 419, 926
BstMBI GATC 7 cut(s) 301, 341, 557, 599, 673, 712, 726
BstMWI GCNNNNNNNGC 3 cut(s) 22, 235, 305
BstNI CCWGG 1 cut(s) 941
BstNSI RCATGY 1 cut(s) 299
BstSCI CCNGG 1 cut(s) 939
BstV1I GCAGC 3 cut(s) 20, 137, 361
BstX2I RGATCY 5 cut(s) 301, 341, 557, 599, 712
BstYI RGATCY 5 cut(s) 301, 341, 557, 599, 712
BsuRI GGCC 3 cut(s) 359, 767, 806
BtgI CCRYGG 1 cut(s) 354
BtsCI GGATG 2 cut(s) 391, 552
BtsIMutI CAGTG 1 cut(s) 87
Cac8I GCNNGC 4 cut(s) 45, 51, 87, 240
CaiI CAGNNNCTG 2 cut(s) 19, 232
CfoI GCGC 1 cut(s) 49
Cfr13I GGNCC 2 cut(s) 99, 192
CseI GACGC 1 cut(s) 943
Csp6I GTAC 1 cut(s) 200
CviAII CATG 4 cut(s) 296, 355, 474, 527
CviQI GTAC 1 cut(s) 200
DdeI CTNAG 5 cut(s) 12, 337, 449, 636, 654
DpnI GATC 7 cut(s) 303, 343, 559, 601, 675, 714, 728
DpnII GATC 7 cut(s) 301, 341, 557, 599, 673, 712, 726
EaeI YGGCCR 1 cut(s) 765
Eam1104I CTCTTC 3 cut(s) 177, 548, 843
EarI CTCTTC 3 cut(s) 177, 548, 843
Eco130I CCWWGG 3 cut(s) 153, 354, 669
Eco47I GGWCC 2 cut(s) 99, 192
Eco57I CTGAAG 2 cut(s) 54, 258
EcoRII CCWGG 1 cut(s) 939
EcoT14I CCWWGG 3 cut(s) 153, 354, 669
EcoT22I ATGCAT 1 cut(s) 790
ErhI CCWWGG 3 cut(s) 153, 354, 669
Esp3I CGTCTC 1 cut(s) 926
FaeI CATG 4 cut(s) 299, 358, 477, 530
FaqI GGGAC 2 cut(s) 85, 861
FatI CATG 4 cut(s) 295, 354, 473, 526
Fnu4HI GCNGC 3 cut(s) 9, 126, 375
FokI GGATG 2 cut(s) 398, 559
Fsp4HI GCNGC 3 cut(s) 9, 126, 375
FspBI CTAG 3 cut(s) 26, 842, 854
GlaI GCGC 1 cut(s) 48
GluI GCNGC 3 cut(s) 9, 126, 375
GsuI CTGGAG 1 cut(s) 254
HaeIII GGCC 3 cut(s) 359, 767, 806
HapII CCGG 1 cut(s) 380
HgaI GACGC 1 cut(s) 943
HhaI GCGC 1 cut(s) 49
Hin1I GRCGYC 1 cut(s) 935
Hin1II CATG 4 cut(s) 299, 358, 477, 530
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HinfI GANTC 4 cut(s) 389, 447, 605, 944
HpaII CCGG 1 cut(s) 380
HphI GGTGA 1 cut(s) 500
Hpy166II GTNNAC 1 cut(s) 316
Hpy188I TCNGA 8 cut(s) 141, 277, 415, 424, 604, 637, 655, 712
Hpy188III TCNNGA 7 cut(s) 233, 260, 339, 746, 780, 842, 948
Hpy8I GTNNAC 1 cut(s) 316
HpyAV CCTTC 4 cut(s) 72, 176, 370, 747
HpyCH4III ACNGT 2 cut(s) 82, 204
HpyCH4IV ACGT 2 cut(s) 198, 219
HpyCH4V TGCA 7 cut(s) 53, 212, 287, 299, 377, 517, 788
HpyF10VI GCNNNNNNNGC 3 cut(s) 22, 235, 305
HpyF3I CTNAG 5 cut(s) 12, 337, 449, 636, 654
HpySE526I ACGT 2 cut(s) 198, 219
Hsp92I GRCGYC 1 cut(s) 935
Hsp92II CATG 4 cut(s) 299, 358, 477, 530
HspAI GCGC 1 cut(s) 47
Kzo9I GATC 7 cut(s) 301, 341, 557, 599, 673, 712, 726
LmnI GCTCC 2 cut(s) 56, 235
Lsp1109I GCAGC 3 cut(s) 20, 137, 361
LweI GCATC 1 cut(s) 797
MaeI CTAG 3 cut(s) 26, 842, 854
MaeII ACGT 2 cut(s) 198, 219
MaeIII GTNAC 1 cut(s) 264
MalI GATC 7 cut(s) 303, 343, 559, 601, 675, 714, 728
MboI GATC 7 cut(s) 301, 341, 557, 599, 673, 712, 726
MboII GAAGA 5 cut(s) 164, 264, 565, 600, 830
MflI RGATCY 5 cut(s) 301, 341, 557, 599, 712
MhlI GDGCHC 2 cut(s) 61, 209
MluCI AATT 7 cut(s) 133, 346, 617, 664, 749, 796, 861
MlyI GAGTC 1 cut(s) 441
MmeI TCCRAC 3 cut(s) 219, 838, 944
MnlI CCTC 9 cut(s) 27, 104, 114, 240, 340, 376, 478, 631, 838
Mph1103I ATGCAT 1 cut(s) 790
MroXI GAANNNNTTC 2 cut(s) 495, 759
MseI TTAA 3 cut(s) 663, 827, 881
MslI CAYNNNNRTG 1 cut(s) 512
MspI CCGG 1 cut(s) 380
MspR9I CCNGG 1 cut(s) 941
MvaI CCWGG 1 cut(s) 941
MwoI GCNNNNNNNGC 3 cut(s) 22, 235, 305
NcoI CCATGG 1 cut(s) 354
NdeII GATC 7 cut(s) 301, 341, 557, 599, 673, 712, 726
NlaIII CATG 4 cut(s) 299, 358, 477, 530
NlaIV GGNNCC 2 cut(s) 101, 343
NsiI ATGCAT 1 cut(s) 790
NspI RCATGY 1 cut(s) 299
OliI CACNNNNGTG 1 cut(s) 512
PdmI GAANNNNTTC 2 cut(s) 495, 759
PfeI GAWTC 2 cut(s) 389, 605
PkrI GCNGC 3 cut(s) 10, 127, 376
PleI GAGTC 1 cut(s) 441
PpsI GAGTC 1 cut(s) 441
PsiI TTATAA 1 cut(s) 875
Psp1406I AACGTT 1 cut(s) 219
Psp6I CCWGG 1 cut(s) 939
PspGI CCWGG 1 cut(s) 939
PspN4I GGNNCC 2 cut(s) 101, 343
PspPI GGNCC 2 cut(s) 99, 192
PstNI CAGNNNCTG 2 cut(s) 19, 232
PsuI RGATCY 5 cut(s) 301, 341, 557, 599, 712
RsaI GTAC 1 cut(s) 201
RsaNI GTAC 1 cut(s) 200
RseI CAYNNNNRTG 1 cut(s) 512
SaqAI TTAA 3 cut(s) 663, 827, 881
SatI GCNGC 3 cut(s) 9, 126, 375
Sau3AI GATC 7 cut(s) 301, 341, 557, 599, 673, 712, 726
Sau96I GGNCC 2 cut(s) 99, 192
SchI GAGTC 1 cut(s) 441
ScrFI CCNGG 1 cut(s) 941
SduI GDGCHC 2 cut(s) 61, 209
SfaNI GCATC 1 cut(s) 797
SinI GGWCC 2 cut(s) 99, 192
SmiMI CAYNNNNRTG 1 cut(s) 512
Sse9I AATT 7 cut(s) 133, 346, 617, 664, 749, 796, 861
SspMI CTAG 3 cut(s) 26, 842, 854
StyD4I CCNGG 1 cut(s) 939
StyI CCWWGG 3 cut(s) 153, 354, 669
TaaI ACNGT 2 cut(s) 82, 204
TaiI ACGT 2 cut(s) 201, 222
TaqI TCGA 3 cut(s) 479, 725, 745
TasI AATT 7 cut(s) 133, 346, 617, 664, 749, 796, 861
TfiI GAWTC 2 cut(s) 389, 605
Tru1I TTAA 3 cut(s) 663, 827, 881
Tru9I TTAA 3 cut(s) 663, 827, 881
TscAI CASTG 1 cut(s) 87
TseI GCWGC 3 cut(s) 8, 125, 374
TspDTI ATGAA 3 cut(s) 17, 212, 828
TspRI CASTG 1 cut(s) 87
VpaK11BI GGWCC 2 cut(s) 99, 192
XapI RAATTY 1 cut(s) 617
XbaI TCTAGA 1 cut(s) 841
XceI RCATGY 1 cut(s) 299
XcmI CCANNNNNNNNNTGG 2 cut(s) 93, 352
XmnI GAANNNNTTC 2 cut(s) 495, 759
XspI CTAG 3 cut(s) 26, 842, 854
Zsp2I ATGCAT 1 cut(s) 790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.