Rw5G050390

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002354
Physical Location & Seq
Forward (+)
86941574 .. 86945033
3460 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G050390.1

Sequence Viewer

Length: 930 bp
ATGAAGGCTGCTCTACCTTTGCTAGCATATTTGGTGGCTTTGTTGCTTGCCTCTCAGATTCCTTCAGGGCTTCCGATGCCCTCCACTTACAACACAGTGCCAGCTTTCCTCTGGTCACCGCACTACCATCAGTATGATTTAGCAAAGTCTGTGCTTTCAGAAGGGGGCTGGTCGAACTTGCTGTGCCCTAGCAATGAAGTTCATCAGCCTTTGGACCTTGCTCTTGTCTTCATTGGTACAGAGTTGCAATCTTCGGATATTTCTGCAAGCAAACATGCTGATCCAGCTCTTGTGGACTTACTCAAAGTCTCTTTCACACAATCAAATTTCTCCATGGCATTTCCCTATATTGCTGCATCAGAGAAGGATGCTATGGAAAGTTCTTTAGTGTCAGGGATGTCAGAGATCTGTGGGCAGAATTTTGGATTCAATAATGTTCGTTTCTCGGAGTCATGCTCTATGGACAGTGAGAATGTTCAAAAGCTTTCAGACCTGCATTCGGTCCATGATTATCTTCTTTCGAGGATGGAGAAAAGATCAAGTGGGGAAGCAGATTTGGTGGTGTTCTGCCACAAAGGATCTGATTCCTCAAAGGAACTAGAACACCCACATTCAGAGAGTGAAATTTTCTCTGAGATTATTAGTTCTGTGGATCAGTCTGGGGCGAAATATGCCGTTCTGTATGTTTCAGACCCCATTAAATCAATCCAATATCCTTCTCATCGAGAGTTAGAAAGGTTTCTCGCTGAAAGTGCCTCAGGAAATGCATCAGCCAATTCGACAGCCTGTGATGAAGTTTGTCAGATCAAATCATCACTTCTGGAGGGACTTCTAGTTGCAATTGTTCTGCTTATAATCTTAATATCAGGCATCTGCTGTATGATGGGCATTGATACTCCCACGAGATTTGAGGCACCCCAAGAGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

309

Amino Acids

33.6

Weight (kDa)

4.77

Isoelectric Point (pI)

51.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ac45-VOA1_TM PF20520 273 - 304 3.9e-09 V0 complex accessory subunit Ac45/VOA1 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 854
Acc36I ACCTGC 1 cut(s) 501
AccB1I GGYRCC 1 cut(s) 913
AciI CCGC 1 cut(s) 119
AclWI GGATC 3 cut(s) 275, 586, 660
AcsI RAATTY 3 cut(s) 325, 418, 624
AcuI CTGAAG 1 cut(s) 48
AfaI GTAC 1 cut(s) 238
AfiI CCNNNNNNNGG 1 cut(s) 499
AgsI TTSAA 2 cut(s) 430, 479
AluBI AGCT 3 cut(s) 104, 287, 484
AluI AGCT 3 cut(s) 104, 287, 484
Alw26I GTCTC 1 cut(s) 313
AlwI GGATC 3 cut(s) 275, 586, 660
ApeKI GCWGC 2 cut(s) 8, 353
ApoI RAATTY 3 cut(s) 325, 418, 624
Asp700I GAANNNNTTC 1 cut(s) 738
AspS9I GGNCC 2 cut(s) 214, 502
AsuHPI GGTGA 1 cut(s) 108
AsuNHI GCTAGC 1 cut(s) 22
AvaII GGWCC 2 cut(s) 214, 502
AxyI CCTNAGG 1 cut(s) 757
BaeGI GKGCMC 1 cut(s) 188
BanI GGYRCC 1 cut(s) 913
BauI CACGAG 1 cut(s) 901
BbsI GAAGAC 1 cut(s) 220
BbvI GCAGC 1 cut(s) 340
BccI CCATC 3 cut(s) 135, 520, 877
BceAI ACGGC 1 cut(s) 659
BcoDI GTCTC 1 cut(s) 313
BfaI CTAG 4 cut(s) 23, 189, 599, 833
BfuAI ACCTGC 1 cut(s) 501
BglII AGATCT 1 cut(s) 405
BisI GCNGC 2 cut(s) 9, 354
BlsI GCNGC 2 cut(s) 10, 355
Bme18I GGWCC 2 cut(s) 214, 502
BmgT120I GGNCC 2 cut(s) 214, 502
BmiI GGNNCC 1 cut(s) 915
BmsI GCATC 5 cut(s) 66, 358, 365, 776, 879
BmtI GCTAGC 1 cut(s) 26
BpiI GAAGAC 1 cut(s) 220
BplI GAGNNNNNCTC 2 cut(s) 440, 472
BpmI CTGGAG 1 cut(s) 842
BsaJI CCNNGG 1 cut(s) 333
Bsc4I CCNNNNNNNGG 1 cut(s) 499
Bse21I CCTNAGG 1 cut(s) 757
Bse3DI GCAATG 1 cut(s) 199
BseDI CCNNGG 1 cut(s) 333
BseGI GGATG 3 cut(s) 373, 402, 531
BseLI CCNNNNNNNGG 1 cut(s) 499
BseMI GCAATG 1 cut(s) 199
BseMII CTCAG 3 cut(s) 68, 624, 771
BseSI GKGCMC 1 cut(s) 188
BseXI GCAGC 1 cut(s) 340
BshNI GGYRCC 1 cut(s) 913
BslFI GGGAC 1 cut(s) 840
BslI CCNNNNNNNGG 1 cut(s) 499
BsmAI GTCTC 1 cut(s) 313
BsmFI GGGAC 1 cut(s) 840
BsmI GAATGC 1 cut(s) 496
Bsp1286I GDGCHC 1 cut(s) 188
Bsp143I GATC 6 cut(s) 280, 405, 536, 578, 652, 804
Bsp19I CCATGG 1 cut(s) 333
BspACI CCGC 1 cut(s) 119
BspCNI CTCAG 3 cut(s) 67, 625, 770
BspLI GGNNCC 1 cut(s) 915
BspMI ACCTGC 1 cut(s) 501
BspOI GCTAGC 1 cut(s) 26
BspPI GGATC 3 cut(s) 275, 586, 660
BspT107I GGYRCC 1 cut(s) 913
BsrDI GCAATG 1 cut(s) 199
BssECI CCNNGG 1 cut(s) 333
BssMI GATC 6 cut(s) 280, 405, 536, 578, 652, 804
BssSI CACGAG 1 cut(s) 901
BssT1I CCWWGG 1 cut(s) 333
Bst2BI CACGAG 1 cut(s) 901
Bst4CI ACNGT 2 cut(s) 97, 467
BstC8I GCNNGC 4 cut(s) 24, 48, 102, 268
BstDEI CTNAG 3 cut(s) 54, 633, 757
BstDSI CCRYGG 1 cut(s) 333
BstEII GGTNACC 1 cut(s) 114
BstF5I GGATG 3 cut(s) 373, 402, 531
BstKTI GATC 6 cut(s) 283, 408, 539, 581, 655, 807
BstMAI GTCTC 1 cut(s) 313
BstMBI GATC 6 cut(s) 280, 405, 536, 578, 652, 804
BstMWI GCNNNNNNNGC 4 cut(s) 76, 284, 671, 752
BstNSI RCATGY 1 cut(s) 278
BstPI GGTNACC 1 cut(s) 114
BstSLI GKGCMC 1 cut(s) 188
BstV1I GCAGC 1 cut(s) 340
BstV2I GAAGAC 1 cut(s) 220
BstX2I RGATCY 2 cut(s) 405, 578
BstYI RGATCY 2 cut(s) 405, 578
Bsu36I CCTNAGG 1 cut(s) 757
BtgI CCRYGG 1 cut(s) 333
BtsCI GGATG 3 cut(s) 373, 402, 531
BtsIMutI CAGTG 2 cut(s) 102, 472
BveI ACCTGC 1 cut(s) 501
Cac8I GCNNGC 4 cut(s) 24, 48, 102, 268
Cfr13I GGNCC 2 cut(s) 214, 502
Csp6I GTAC 1 cut(s) 237
CspCI CAANNNNNGTGG 2 cut(s) 889, 924
CviAII CATG 4 cut(s) 275, 334, 453, 506
CviQI GTAC 1 cut(s) 237
DdeI CTNAG 3 cut(s) 54, 633, 757
DpnI GATC 6 cut(s) 282, 407, 538, 580, 654, 806
DpnII GATC 6 cut(s) 280, 405, 536, 578, 652, 804
Eco130I CCWWGG 1 cut(s) 333
Eco47I GGWCC 2 cut(s) 214, 502
Eco57I CTGAAG 1 cut(s) 48
Eco81I CCTNAGG 1 cut(s) 757
Eco91I GGTNACC 1 cut(s) 114
EcoO65I GGTNACC 1 cut(s) 114
EcoT14I CCWWGG 1 cut(s) 333
EcoT22I ATGCAT 1 cut(s) 769
ErhI CCWWGG 1 cut(s) 333
FaeI CATG 4 cut(s) 278, 337, 456, 509
FaqI GGGAC 1 cut(s) 840
FatI CATG 4 cut(s) 274, 333, 452, 505
Fnu4HI GCNGC 2 cut(s) 9, 354
FokI GGATG 3 cut(s) 380, 409, 538
Fsp4HI GCNGC 2 cut(s) 9, 354
FspBI CTAG 4 cut(s) 23, 189, 599, 833
GluI GCNGC 2 cut(s) 9, 354
GsuI CTGGAG 1 cut(s) 842
Hin1II CATG 4 cut(s) 278, 337, 456, 509
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 5 cut(s) 58, 426, 449, 584, 923
HphI GGTGA 1 cut(s) 108
Hpy166II GTNNAC 1 cut(s) 295
Hpy188III TCNNGA 3 cut(s) 725, 759, 821
Hpy8I GTNNAC 1 cut(s) 295
HpyAV CCTTC 4 cut(s) 72, 155, 358, 726
HpyCH4III ACNGT 2 cut(s) 97, 467
HpyCH4V TGCA 6 cut(s) 247, 266, 356, 496, 767, 839
HpyF10VI GCNNNNNNNGC 4 cut(s) 76, 284, 671, 752
HpyF3I CTNAG 3 cut(s) 54, 633, 757
Hsp92II CATG 4 cut(s) 278, 337, 456, 509
Kzo9I GATC 6 cut(s) 280, 405, 536, 578, 652, 804
Lsp1109I GCAGC 1 cut(s) 340
LweI GCATC 5 cut(s) 66, 358, 365, 776, 879
MaeI CTAG 4 cut(s) 23, 189, 599, 833
MaeIII GTNAC 1 cut(s) 114
MalI GATC 6 cut(s) 282, 407, 538, 580, 654, 806
MboI GATC 6 cut(s) 280, 405, 536, 578, 652, 804
MboII GAAGA 3 cut(s) 220, 243, 506
MfeI CAATTG 1 cut(s) 840
MflI RGATCY 2 cut(s) 405, 578
MhlI GDGCHC 1 cut(s) 188
MluCI AATT 5 cut(s) 325, 418, 624, 775, 840
MlyI GAGTC 1 cut(s) 458
MnlI CCTC 8 cut(s) 61, 91, 119, 516, 598, 766, 817, 904
Mph1103I ATGCAT 1 cut(s) 769
MroXI GAANNNNTTC 1 cut(s) 738
MseI TTAA 3 cut(s) 699, 860, 928
MslI CAYNNNNRTG 1 cut(s) 132
MunI CAATTG 1 cut(s) 840
Mva1269I GAATGC 1 cut(s) 496
MwoI GCNNNNNNNGC 4 cut(s) 76, 284, 671, 752
NcoI CCATGG 1 cut(s) 333
NdeII GATC 6 cut(s) 280, 405, 536, 578, 652, 804
NheI GCTAGC 1 cut(s) 22
NlaIII CATG 4 cut(s) 278, 337, 456, 509
NlaIV GGNNCC 1 cut(s) 915
NmuCI GTSAC 1 cut(s) 114
NsiI ATGCAT 1 cut(s) 769
NspI RCATGY 1 cut(s) 278
PctI GAATGC 1 cut(s) 496
PdmI GAANNNNTTC 1 cut(s) 738
PfeI GAWTC 3 cut(s) 58, 426, 584
PkrI GCNGC 2 cut(s) 10, 355
PleI GAGTC 1 cut(s) 457
PpsI GAGTC 1 cut(s) 457
PsiI TTATAA 1 cut(s) 854
PspEI GGTNACC 1 cut(s) 114
PspN4I GGNNCC 1 cut(s) 915
PspPI GGNCC 2 cut(s) 214, 502
PsuI RGATCY 2 cut(s) 405, 578
RsaI GTAC 1 cut(s) 238
RsaNI GTAC 1 cut(s) 237
RseI CAYNNNNRTG 1 cut(s) 132
SaqAI TTAA 3 cut(s) 699, 860, 928
SatI GCNGC 2 cut(s) 9, 354
Sau3AI GATC 6 cut(s) 280, 405, 536, 578, 652, 804
Sau96I GGNCC 2 cut(s) 214, 502
SchI GAGTC 1 cut(s) 458
SduI GDGCHC 1 cut(s) 188
SetI ASST 7 cut(s) 19, 106, 219, 289, 486, 495, 740
SfaNI GCATC 5 cut(s) 66, 358, 365, 776, 879
SinI GGWCC 2 cut(s) 214, 502
SmiMI CAYNNNNRTG 1 cut(s) 132
Sse9I AATT 5 cut(s) 325, 418, 624, 775, 840
SsiI CCGC 1 cut(s) 119
SspMI CTAG 4 cut(s) 23, 189, 599, 833
StyI CCWWGG 1 cut(s) 333
TaaI ACNGT 2 cut(s) 97, 467
TaqI TCGA 4 cut(s) 173, 521, 724, 779
TaqII GACCGA 1 cut(s) 490
TasI AATT 5 cut(s) 325, 418, 624, 775, 840
TfiI GAWTC 3 cut(s) 58, 426, 584
Tru1I TTAA 3 cut(s) 699, 860, 928
Tru9I TTAA 3 cut(s) 699, 860, 928
TscAI CASTG 2 cut(s) 102, 472
TseFI GTSAC 1 cut(s) 114
TseI GCWGC 2 cut(s) 8, 353
Tsp45I GTSAC 1 cut(s) 114
TspDTI ATGAA 5 cut(s) 17, 191, 210, 220, 807
TspRI CASTG 2 cut(s) 102, 472
VpaK11BI GGWCC 2 cut(s) 214, 502
XapI RAATTY 3 cut(s) 325, 418, 624
XceI RCATGY 1 cut(s) 278
XcmI CCANNNNNNNNNTGG 1 cut(s) 108
XmnI GAANNNNTTC 1 cut(s) 738
XspI CTAG 4 cut(s) 23, 189, 599, 833
Zsp2I ATGCAT 1 cut(s) 769
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.