Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0002584

Physical Location & Seq

Genomic Coordinates Forward (+)

7296213 .. 7301468

Gene Structure 5256 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1088300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2439 bp

ATGATTTTTTCTCGGATTGGTCGCTCCGTTTCTCGATCTTCTCGCGCCAGGAATTTGTTGCTTGGAAGTGGGCGATCGGCGGGTCTGATTGGGAATGGAGGCATTTCTGGAGTTCCGCGTTTTGGTTCGTATCTGGGTCCGGTCGATGGGGAATTAGGGTTTTTGAGGTCGTATTTTGCTGCGTCAATAGGGGCCCACAAGGCCTGCGTTTCGGATTTCAGTTGCATTCTTGCAAACCCTAAACTTAGCCGACACTTTTCGAGTGAAACCCCAAAGAAAAAGAATTATGAGAACTTCTATCCCAAGGAAAAGAAGGAAATTCCGAAAGGAGATGAGCAGAAATCGGAATCAAAAGATGAATCAAAGGCAGATGATCAGGGGAGTTTTCAGGAAACTTTCTTGAGGCAGTTTCAAAATTTAATTACCCCGCTATTGGTCATTGGGCTATTTCTTTCCTCCTTCTCCTTTGGTACTCCTGATCAGCAACAGATAAGTTTCCAAGAGTTTAAAAACAAACTTCTGGAACCAGGCTTAGTGGATCATATAGTTGTCTCTAATAAATCAGTTGCAAAAGTGTATGTGCGTAGCTCGCCACGCGGTCAGACAAGTGAGGAAGTTGTCCAAGGACCTGGTACCCCTGCTCGTGCAAATGGGGGCCAGTACAAATACTATTTCAATATTGGAAGTGTTGAATCTTTTGAGGAGAAGTTGGAGGATGCCCAAGAAGCGTTGGGAATAGACAGTCATGATTTTGTTCCTGTTACATATGTCTCTGAGATGGTTTGGTACCAAGAATTGATGAGATTTGCACCAACACTACTGCTTTTGGGATCCCTCTTGTTTATGGGGCGGAGAATGCAGGGTGGACTAGGTATTGGTGGTTCTGGCGGACGAAGTGGCCGTGGAATCTTCAATATAGGAAAAGCCCAAGTTACAAAAGTAGATAAAAATACAAAGAACAAGATCTATTTTAAAGATGTTGCTGGTTGCGATGAAGCAAAGCAGGAAATCATGGAATTTGTGCACTTTCTCAAGAATCCTAAGAAATATGAGGATTTGGGAGCCAAAATTCCCAAAGGAGCTCTATTGGTAGGCCCTCCAGGCACTGGAAAGACCCTTCTAGCAAAAGCAACTGCAGGGGAATCTGGTGTTCCATTTCTATCTATGGCTGGATCAGACTTCATGGAGATGTTCGTTGGTGTTGGACCGTCAAGAGTCAGAAACTTGTTTCAGGAAGCAAGGCAATGTGCTCCTAGTATAATATTTATTGATGAGATTGATGCAATTGGTCGAGCAAGGGGGCGAGGGGGCTTGTCAGGTTCCAATGATGAGCGTGAGAGTACTCTAAATCAGTTGTTGGTAGAAATGGATGGCTTTGGAACAACTGCTGGAGTAGTTGTTCTTGCTGGCACCAATAGGCCTGATATTTTGGACAAAGCCTTATTAAGGCCTGGTCGATTTGATCGCCAAATCTCTATTGATAAACCTGATATTAAAGGTCGTGACCAAATATTTCAGATATACTTGAAAAAGATCAAACTTGATCATGAACCATCATATTATTCTCAAAGGCTTGCAGCCCTAACTCCAGGGTTTGCTGGGGCAGACATTGCAAATGTTTGCAATGAAGGTGCTCTAATTGCTGCAAGAAATGAGAGTGCACTGGTCACAATGCAGCATTTTGAGGCGGCTATAGACAGAATTATTGGTGGTTTGGAGAAGAAGAACAAGGTTATAAGCAAGCTCGAGCGCCGTACCGTTGCCTACCATGAGGCAGGGCATGCTGTTACCGGATGGTTCTTAGAACATGCAGAACCATTGTTGAAAGTGACCATTGTTCCTCGAGGTACTGCAGCACTTGGGTTTGCGCAGTATGTTCCAAATGAAAACCTCCTCATGACCAAGGAGCAGCTTTTTGATATGACCTGCATGACCCTCGGTGGTAGAGCAGCTGAACAGGTTATGTTGGGGAAAATCTCGACGGGGGCCCAAAATGATCTGGAGAAAGTGACAAAGATGACCTACGCCCAAGTTGCAGTCTATGGTTTCAGTGACAAGGTGGGTCTCCTTTCTTTCCCTCAAAGGGAAGATTCATTTGAGATGTCAAAGCCCTACAGCAGCAAAACTGGAGCCCTTATTGATGGTGAAGTGCGCGAATGGGTGGGTAAGGCGTATGCACGCACAGTTGAACTCGTAGAGGAACATAAAGAGCAAATAGCTCAAATTGCAGAATTGTTGCTTGAAAAGGAAGTTCTTCACCAAGATGACCTGCTCAAGGTGTTGGGGGAAAGACCATATAAACCGGCTGAAGTGACCAACTATGATAGATTTAAGGAAGGTTTCGAAGAGAAAAATGATGAGAAAACAGTGGAGACACCGCTGGTTGGGTCCGAGGATGACGGCTCATCACCCCTTGAGCCTCAGGTTCTGCCTACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004176 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009719 GO:0009725 GO:0009735 GO:0010033 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019538 GO:0031976 GO:0031984 GO:0034357 GO:0042221 GO:0042623 GO:0042651 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0055035 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA FtsH_ext Peptidase_M41

Protein Analysis

813

Amino Acids

89.22

Weight (kDa)

6.39

Isoelectric Point (pI)

32.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FtsH_ext	PF06480	142 - 249	6.4e-12	FtsH Extracellular
AAA	PF00004	362 - 494	5.3e-42	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	524 - 563	1.3e-10	AAA+ lid domain
Peptidase_M41	PF01434	578 - 759	1.2e-64	Peptidase family M41

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002584)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07510 AT2G29080
fragaria_vesca	FvH4_3g36790 FvH4_3g36790 FvH4_3g36790 FvH4_6g13480 FvH4_6g13480 FvH4_6g13480
malus_domestica	MD03G1088300.v1.1 MD03G1088400.v1.1 MD04G1130900.v1.1 MD11G1097400.v1.1 MD12G1144000.v1.1
prunus_persica	Prupe.6G072600_v2.0.a1 Prupe.6G257400_v2.0.a1 Prupe.6G257400_v2.0.a1
pyrus_communis	pycom03g06980 pycom03g07000 pycom04g11920 pycom11g08210 pycom12g13860
rosa_chinensis	RchiOBHm_Chr3g0466351 RchiOBHm_Chr5g0065851
rosa_laevigata	RLG00000024582 RLG00000035821
rosa_multiflora	Rmu_sc0003480.1_g000012 Rmu_sc0042868.1_g000001
rosa_roxburghii	Rroxscaffold_1G00015310 Rroxscaffold_6G00414550
rosa_rugosa	Rorug03G0081200 Rorug05G0374100 Rorug05G0374200 Rorug05G0374300
rosa_samantha	Rh3BG148900 Rh3DG149600 Rh5BG449200 Rh5CG471400 Rh5DG462800
rosa_wichuraiana	Rw3G012050 Rw5G040600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1736
AbsI	CCTCGAGG	1 cut(s)	1842
Acc16I	TGCGCA	1 cut(s)	1869
Acc36I	ACCTGC	2 cut(s)	1934, 2277
Acc65I	GGTACC	2 cut(s)	632, 786
AccB1I	GGYRCC	3 cut(s)	632, 786, 1409
AccB7I	CCANNNNNTGG	1 cut(s)	1106
AccII	CGCG	4 cut(s)	45, 118, 597, 2154
AciI	CCGC	8 cut(s)	80, 116, 428, 597, 850, 888, 1688, 2378
AclWI	GGATC	4 cut(s)	546, 825, 838, 1180
AcoI	YGGCCR	1 cut(s)	898
AcsI	RAATTY	5 cut(s)	52, 318, 415, 1016, 1068
AcuI	CTGAAG	1 cut(s)	2328
AfaI	GTAC	7 cut(s)	472, 634, 662, 788, 1342, 1756, 1849
AfiI	CCNNNNNNNGG	8 cut(s)	122, 146, 433, 1106, 1446, 2083, 2275, 2384
AgsI	TTSAA	8 cut(s)	413, 676, 692, 913, 1528, 1825, 2189, 2243
AjnI	CCWGG	6 cut(s)	47, 526, 628, 1099, 1450, 1588
AloI	GAACNNNNNNTCC	2 cut(s)	1383, 1415
AluBI	AGCT	6 cut(s)	588, 1082, 1744, 1912, 1952, 2219
AluI	AGCT	6 cut(s)	588, 1082, 1744, 1912, 1952, 2219
Alw21I	GWGCWC	5 cut(s)	1026, 1084, 1252, 1636, 1663
Alw26I	GTCTC	4 cut(s)	556, 775, 2069, 2366
Alw44I	GTGCAC	2 cut(s)	1022, 1659
AlwI	GGATC	4 cut(s)	546, 825, 838, 1180
AlwNI	CAGNNNCTG	2 cut(s)	1106, 2428
Ama87I	CYCGRG	2 cut(s)	1745, 1842
AoxI	GGCC	8 cut(s)	192, 201, 655, 898, 1093, 1418, 1448, 1986
ApaI	GGGCCC	2 cut(s)	196, 1990
ApaLI	GTGCAC	2 cut(s)	1022, 1659
ApeKI	GCWGC	8 cut(s)	179, 1577, 1643, 1675, 1853, 1909, 1949, 2118
ApoI	RAATTY	5 cut(s)	52, 318, 415, 1016, 1068
Asp700I	GAANNNNTTC	1 cut(s)	2253
Asp718I	GGTACC	2 cut(s)	632, 786
AspLEI	GCGC	4 cut(s)	47, 1752, 1870, 2154
AsuHPI	GGTGA	3 cut(s)	2156, 2249, 2400
AsuII	TTCGAA	1 cut(s)	2343
AvaI	CYCGRG	2 cut(s)	1745, 1842
AvaII	GGWCC	4 cut(s)	137, 626, 1205, 2388
AxyI	CCTNAGG	1 cut(s)	2421
BaeGI	GKGCMC	4 cut(s)	196, 1026, 1663, 1990
BamHI	GGATCC	1 cut(s)	830
BanI	GGYRCC	3 cut(s)	632, 786, 1409
BanII	GRGCYC	4 cut(s)	196, 1084, 1990, 2134
BauI	CACGAG	1 cut(s)	642
Bbv12I	GWGCWC	5 cut(s)	1026, 1084, 1252, 1636, 1663
BbvI	GCAGC	8 cut(s)	166, 1589, 1630, 1687, 1865, 1921, 1961, 2130
BccI	CCATC	6 cut(s)	140, 772, 1364, 1561, 1788, 2135
BceAI	ACGGC	3 cut(s)	885, 1737, 2416
BcgI	CGANNNNNNTGC	2 cut(s)	1918, 1952
BciT130I	CCWGG	6 cut(s)	49, 528, 630, 1101, 1452, 1590
BclI	TGATCA	3 cut(s)	373, 478, 1543
BcoDI	GTCTC	4 cut(s)	556, 775, 2069, 2366
BfaI	CTAG	3 cut(s)	869, 1121, 1254
BfmI	CTRYAG	4 cut(s)	1134, 1692, 1851, 2113
BfoI	RGCGCY	1 cut(s)	1753
BfuAI	ACCTGC	2 cut(s)	1934, 2277
BglI	GCCNNNNNGGC	2 cut(s)	200, 1101
BglII	AGATCT	1 cut(s)	963
BisI	GCNGC	9 cut(s)	180, 1578, 1644, 1676, 1689, 1854, 1910, 1950, 2119
BlsI	GCNGC	9 cut(s)	181, 1579, 1645, 1677, 1690, 1855, 1911, 1951, 2120
BmcAI	AGTACT	1 cut(s)	1342
Bme1390I	CCNGG	6 cut(s)	49, 528, 630, 1101, 1452, 1590
Bme18I	GGWCC	4 cut(s)	137, 626, 1205, 2388
BmeT110I	CYCGRG	2 cut(s)	1745, 1842
BmrFI	CCNGG	6 cut(s)	49, 528, 630, 1101, 1452, 1590
BmsI	GCATC	2 cut(s)	706, 1270
BpmI	CTGGAG	6 cut(s)	129, 1083, 1410, 1572, 2021, 2148
Bpu14I	TTCGAA	1 cut(s)	2343
BpuEI	CTTGAG	4 cut(s)	421, 1016, 2258, 2435
BsaI	GGTCTC	1 cut(s)	2069
BsaJI	CCNNGG	7 cut(s)	303, 622, 901, 1589, 1902, 1936, 2391
BsaWI	WCCGGW	2 cut(s)	139, 1790
BsaXI	ACNNNNNCTCC	2 cut(s)	1383, 1413
Bsc4I	CCNNNNNNNGG	8 cut(s)	122, 146, 433, 1106, 1446, 2083, 2275, 2384
Bse118I	RCCGGY	1 cut(s)	2302
Bse1I	ACTGG	4 cut(s)	658, 1111, 1668, 2131
Bse21I	CCTNAGG	1 cut(s)	2421
Bse3DI	GCAATG	3 cut(s)	1250, 1608, 1630
BseBI	CCWGG	6 cut(s)	49, 528, 630, 1101, 1452, 1590
BseDI	CCNNGG	7 cut(s)	303, 622, 901, 1589, 1902, 1936, 2391
BseGI	GGATG	4 cut(s)	721, 1375, 1799, 2401
BseLI	CCNNNNNNNGG	8 cut(s)	122, 146, 433, 1106, 1446, 2083, 2275, 2384
BseMI	GCAATG	3 cut(s)	1250, 1608, 1630
BseMII	CTCAG	2 cut(s)	765, 2435
BseNI	ACTGG	4 cut(s)	658, 1111, 1668, 2131
BseRI	GAGGAG	2 cut(s)	716, 1883
BseSI	GKGCMC	4 cut(s)	196, 1026, 1663, 1990
BseXI	GCAGC	8 cut(s)	166, 1589, 1630, 1687, 1865, 1921, 1961, 2130
BseYI	CCCAGC	1 cut(s)	1598
Bsh1236I	CGCG	4 cut(s)	45, 118, 597, 2154
Bsh1285I	CGRYCG	2 cut(s)	77, 144
BshFI	GGCC	8 cut(s)	194, 203, 657, 900, 1095, 1420, 1450, 1988
BshNI	GGYRCC	3 cut(s)	632, 786, 1409
BsiEI	CGRYCG	2 cut(s)	77, 144
BsiHKAI	GWGCWC	5 cut(s)	1026, 1084, 1252, 1636, 1663
BsiHKCI	CYCGRG	2 cut(s)	1745, 1842
BsiSI	CCGG	3 cut(s)	140, 1791, 2303
BslI	CCNNNNNNNGG	8 cut(s)	122, 146, 433, 1106, 1446, 2083, 2275, 2384
BsmAI	GTCTC	4 cut(s)	556, 775, 2069, 2366
BsmI	GAATGC	2 cut(s)	225, 861
BsnI	GGCC	8 cut(s)	194, 203, 657, 900, 1095, 1420, 1450, 1988
Bso31I	GGTCTC	1 cut(s)	2069
BsoBI	CYCGRG	2 cut(s)	1745, 1842
Bsp119I	TTCGAA	1 cut(s)	2343
Bsp120I	GGGCCC	2 cut(s)	192, 1986
Bsp1286I	GDGCHC	8 cut(s)	196, 1026, 1084, 1252, 1636, 1663, 1990, 2134
BspACI	CCGC	8 cut(s)	80, 116, 428, 597, 850, 888, 1688, 2378
BspANI	GGCC	8 cut(s)	194, 203, 657, 900, 1095, 1420, 1450, 1988
BspCNI	CTCAG	2 cut(s)	766, 2434
BspFNI	CGCG	4 cut(s)	45, 118, 597, 2154
BspHI	TCATGA	3 cut(s)	745, 1546, 1896
BspMAI	CTGCAG	2 cut(s)	1138, 1855
BspMI	ACCTGC	2 cut(s)	1934, 2277
BspPI	GGATC	4 cut(s)	546, 825, 838, 1180
BspT104I	TTCGAA	1 cut(s)	2343
BspT107I	GGYRCC	3 cut(s)	632, 786, 1409
BspTNI	GGTCTC	1 cut(s)	2069
BsrDI	GCAATG	3 cut(s)	1250, 1608, 1630
BsrFI	RCCGGY	1 cut(s)	2302
BsrI	ACTGG	4 cut(s)	658, 1111, 1668, 2131
BssAI	RCCGGY	1 cut(s)	2302
BssECI	CCNNGG	7 cut(s)	303, 622, 901, 1589, 1902, 1936, 2391
BssSI	CACGAG	1 cut(s)	642
BssT1I	CCWWGG	3 cut(s)	303, 622, 1902
Bst2BI	CACGAG	1 cut(s)	642
Bst2UI	CCWGG	6 cut(s)	49, 528, 630, 1101, 1452, 1590
Bst4CI	ACNGT	5 cut(s)	743, 1209, 1759, 2185, 2368
Bst6I	CTCTTC	1 cut(s)	2340
BstAPI	GCANNNNNTGC	2 cut(s)	1610, 1781
BstBI	TTCGAA	1 cut(s)	2343
BstC8I	GCNNGC	7 cut(s)	205, 590, 1408, 1575, 1742, 1782, 2179
BstDEI	CTNAG	7 cut(s)	245, 532, 774, 1041, 1801, 2421, 2436
BstDSI	CCRYGG	1 cut(s)	901
BstENI	CCTNNNNNAGG	2 cut(s)	1444, 2273
BstF5I	GGATG	4 cut(s)	721, 1375, 1799, 2401
BstFNI	CGCG	4 cut(s)	45, 118, 597, 2154
BstH2I	RGCGCY	1 cut(s)	1753
BstHHI	GCGC	4 cut(s)	47, 1752, 1870, 2154
BstMAI	GTCTC	4 cut(s)	556, 775, 2069, 2366
BstMCI	CGRYCG	2 cut(s)	77, 144
BstNI	CCWGG	6 cut(s)	49, 528, 630, 1101, 1452, 1590
BstNSI	RCATGY	2 cut(s)	1784, 1811
BstSCI	CCNGG	6 cut(s)	47, 526, 628, 1099, 1450, 1588
BstSFI	CTRYAG	4 cut(s)	1134, 1692, 1851, 2113
BstSLI	GKGCMC	4 cut(s)	196, 1026, 1663, 1990
BstUI	CGCG	4 cut(s)	45, 118, 597, 2154
BstV1I	GCAGC	8 cut(s)	166, 1589, 1630, 1687, 1865, 1921, 1961, 2130
BstX2I	RGATCY	2 cut(s)	830, 963
BstXI	CCANNNNNNTGG	1 cut(s)	629
BstYI	RGATCY	2 cut(s)	830, 963
Bsu36I	CCTNAGG	1 cut(s)	2421
BsuRI	GGCC	8 cut(s)	194, 203, 657, 900, 1095, 1420, 1450, 1988
BtgI	CCRYGG	1 cut(s)	901
BtgZI	GCGATG	1 cut(s)	1005
BtsCI	GGATG	4 cut(s)	721, 1375, 1799, 2401
BtsIMutI	CAGTG	4 cut(s)	1104, 1661, 2056, 2373
BveI	ACCTGC	2 cut(s)	1934, 2277
Cac8I	GCNNGC	7 cut(s)	205, 590, 1408, 1575, 1742, 1782, 2179
CaiI	CAGNNNCTG	2 cut(s)	1106, 2428
CciI	TCATGA	3 cut(s)	745, 1546, 1896
CfoI	GCGC	4 cut(s)	47, 1752, 1870, 2154
Cfr10I	RCCGGY	1 cut(s)	2302
CseI	GACGC	1 cut(s)	171
CsiI	ACCWGGT	1 cut(s)	628
Csp6I	GTAC	7 cut(s)	471, 633, 661, 787, 1341, 1755, 1848
CviAII	CATG	9 cut(s)	746, 1012, 1183, 1547, 1769, 1781, 1808, 1897, 1930
CviQI	GTAC	7 cut(s)	471, 633, 661, 787, 1341, 1755, 1848
DdeI	CTNAG	7 cut(s)	245, 532, 774, 1041, 1801, 2421, 2436
DraI	TTTAAA	2 cut(s)	508, 973
EaeI	YGGCCR	1 cut(s)	898
Eam1104I	CTCTTC	1 cut(s)	2340
EarI	CTCTTC	1 cut(s)	2340
EciI	GGCGGA	2 cut(s)	865, 903
Ecl136II	GAGCTC	1 cut(s)	1082
Eco130I	CCWWGG	3 cut(s)	303, 622, 1902
Eco147I	AGGCCT	3 cut(s)	203, 1420, 1450
Eco24I	GRGCYC	4 cut(s)	196, 1084, 1990, 2134
Eco31I	GGTCTC	1 cut(s)	2069
Eco47I	GGWCC	4 cut(s)	137, 626, 1205, 2388
Eco53kI	GAGCTC	1 cut(s)	1082
Eco57I	CTGAAG	1 cut(s)	2328
Eco81I	CCTNAGG	1 cut(s)	2421
Eco88I	CYCGRG	2 cut(s)	1745, 1842
EcoICRI	GAGCTC	1 cut(s)	1082
EcoNI	CCTNNNNNAGG	2 cut(s)	1444, 2273
EcoO109I	RGGNCCY	4 cut(s)	192, 626, 1094, 1986
EcoRII	CCWGG	6 cut(s)	47, 526, 628, 1099, 1450, 1588
EcoT14I	CCWWGG	3 cut(s)	303, 622, 1902
EcoT38I	GRGCYC	4 cut(s)	196, 1084, 1990, 2134
ErhI	CCWWGG	3 cut(s)	303, 622, 1902
FaeI	CATG	9 cut(s)	749, 1015, 1186, 1550, 1772, 1784, 1811, 1900, 1933
FatI	CATG	9 cut(s)	745, 1011, 1182, 1546, 1768, 1780, 1807, 1896, 1929
FauI	CCCGC	2 cut(s)	73, 435
FauNDI	CATATG	1 cut(s)	766
FbaI	TGATCA	3 cut(s)	373, 478, 1543
Fnu4HI	GCNGC	9 cut(s)	180, 1578, 1644, 1676, 1689, 1854, 1910, 1950, 2119
FokI	GGATG	4 cut(s)	728, 1382, 1806, 2408
FriOI	GRGCYC	4 cut(s)	196, 1084, 1990, 2134
Fsp4HI	GCNGC	9 cut(s)	180, 1578, 1644, 1676, 1689, 1854, 1910, 1950, 2119
FspBI	CTAG	3 cut(s)	869, 1121, 1254
FspI	TGCGCA	1 cut(s)	1869
GlaI	GCGC	4 cut(s)	46, 1751, 1869, 2153
GluI	GCNGC	9 cut(s)	180, 1578, 1644, 1676, 1689, 1854, 1910, 1950, 2119
GsaI	CCCAGC	1 cut(s)	1602
GsuI	CTGGAG	6 cut(s)	129, 1083, 1410, 1572, 2021, 2148
HaeII	RGCGCY	1 cut(s)	1753
HaeIII	GGCC	8 cut(s)	194, 203, 657, 900, 1095, 1420, 1450, 1988
HapII	CCGG	3 cut(s)	140, 1791, 2303
HgaI	GACGC	1 cut(s)	171
HhaI	GCGC	4 cut(s)	47, 1752, 1870, 2154
Hin1II	CATG	9 cut(s)	749, 1015, 1186, 1550, 1772, 1784, 1811, 1900, 1933
Hin6I	GCGC	4 cut(s)	45, 1750, 1868, 2152
HinP1I	GCGC	4 cut(s)	45, 1750, 1868, 2152
HinfI	GANTC	8 cut(s)	347, 359, 692, 906, 1036, 1142, 1215, 2090
HpaII	CCGG	3 cut(s)	140, 1791, 2303
HphI	GGTGA	3 cut(s)	2156, 2249, 2400
Hpy166II	GTNNAC	3 cut(s)	866, 1024, 1661
Hpy8I	GTNNAC	3 cut(s)	866, 1024, 1661
Hpy99I	CGWCG	1 cut(s)	1984
HpyAV	CCTTC	5 cut(s)	307, 469, 1127, 1622, 2330
HpyCH4III	ACNGT	5 cut(s)	743, 1209, 1759, 2185, 2368
HpyF3I	CTNAG	7 cut(s)	245, 532, 774, 1041, 1801, 2421, 2436
Hsp92II	CATG	9 cut(s)	749, 1015, 1186, 1550, 1772, 1784, 1811, 1900, 1933
HspAI	GCGC	4 cut(s)	45, 1750, 1868, 2152
KpnI	GGTACC	2 cut(s)	636, 790
Ksp22I	TGATCA	3 cut(s)	373, 478, 1543
LmnI	GCTCC	6 cut(s)	29, 1061, 1079, 1255, 1906, 2129
Lsp1109I	GCAGC	8 cut(s)	166, 1589, 1630, 1687, 1865, 1921, 1961, 2130
LweI	GCATC	2 cut(s)	706, 1270
MabI	ACCWGGT	1 cut(s)	628
MaeI	CTAG	3 cut(s)	869, 1121, 1254
MaeIII	GTNAC	9 cut(s)	760, 931, 1502, 1666, 1786, 1828, 2008, 2051, 2311
MboII	GAAGA	7 cut(s)	30, 901, 1732, 1735, 2099, 2246, 2357
MfeI	CAATTG	1 cut(s)	1284
MflI	RGATCY	2 cut(s)	830, 963
MhlI	GDGCHC	8 cut(s)	196, 1026, 1084, 1252, 1636, 1663, 1990, 2134
MlyI	GAGTC	1 cut(s)	1224
MmeI	TCCRAC	2 cut(s)	690, 1183
MroXI	GAANNNNTTC	1 cut(s)	2253
MseI	TTAA	6 cut(s)	419, 507, 972, 1445, 1494, 2331
MslI	CAYNNNNRTG	2 cut(s)	1187, 2262
MspA1I	CMGCKG	2 cut(s)	1952, 2380
MspI	CCGG	3 cut(s)	140, 1791, 2303
MspR9I	CCNGG	6 cut(s)	49, 528, 630, 1101, 1452, 1590
MunI	CAATTG	1 cut(s)	1284
Mva1269I	GAATGC	2 cut(s)	225, 861
MvaI	CCWGG	6 cut(s)	49, 528, 630, 1101, 1452, 1590
MvnI	CGCG	4 cut(s)	45, 118, 597, 2154
NdeI	CATATG	1 cut(s)	766
NlaIII	CATG	9 cut(s)	749, 1015, 1186, 1550, 1772, 1784, 1811, 1900, 1933
NmuCI	GTSAC	6 cut(s)	1502, 1666, 1828, 2008, 2051, 2311
NsbI	TGCGCA	1 cut(s)	1869
NspI	RCATGY	2 cut(s)	1784, 1811
NspV	TTCGAA	1 cut(s)	2343
PaeI	GCATGC	1 cut(s)	1784
PaeR7I	CTCGAG	2 cut(s)	1745, 1842
PagI	TCATGA	3 cut(s)	745, 1546, 1896
PceI	AGGCCT	3 cut(s)	203, 1420, 1450
PcsI	WCGNNNNNNNCGW	1 cut(s)	898
PctI	GAATGC	2 cut(s)	225, 861
PdmI	GAANNNNTTC	1 cut(s)	2253
PfeI	GAWTC	7 cut(s)	347, 359, 692, 906, 1036, 1142, 2090
PflMI	CCANNNNNTGG	1 cut(s)	1106
PkrI	GCNGC	9 cut(s)	181, 1579, 1645, 1677, 1690, 1855, 1911, 1951, 2120
Ple19I	CGATCG	1 cut(s)	77
PleI	GAGTC	1 cut(s)	1223
PpsI	GAGTC	1 cut(s)	1223
PpuMI	RGGWCCY	1 cut(s)	626
PsiI	TTATAA	1 cut(s)	1736
Psp124BI	GAGCTC	1 cut(s)	1084
Psp5II	RGGWCCY	1 cut(s)	626
Psp6I	CCWGG	6 cut(s)	47, 526, 628, 1099, 1450, 1588
PspFI	CCCAGC	1 cut(s)	1598
PspGI	CCWGG	6 cut(s)	47, 526, 628, 1099, 1450, 1588
PspOMI	GGGCCC	2 cut(s)	192, 1986
PspPPI	RGGWCCY	1 cut(s)	626
PspXI	VCTCGAGB	2 cut(s)	1745, 1842
PsrI	GAACNNNNNNTAC	2 cut(s)	865, 897
PstI	CTGCAG	2 cut(s)	1138, 1855
PstNI	CAGNNNCTG	2 cut(s)	1106, 2428
PsuI	RGATCY	2 cut(s)	830, 963
PvuI	CGATCG	1 cut(s)	77
PvuII	CAGCTG	1 cut(s)	1952
RsaI	GTAC	7 cut(s)	472, 634, 662, 788, 1342, 1756, 1849
RsaNI	GTAC	7 cut(s)	471, 633, 661, 787, 1341, 1755, 1848
RseI	CAYNNNNRTG	2 cut(s)	1187, 2262
SacI	GAGCTC	1 cut(s)	1084
SaqAI	TTAA	6 cut(s)	419, 507, 972, 1445, 1494, 2331
SatI	GCNGC	9 cut(s)	180, 1578, 1644, 1676, 1689, 1854, 1910, 1950, 2119
ScaI	AGTACT	1 cut(s)	1342
SchI	GAGTC	1 cut(s)	1224
ScrFI	CCNGG	6 cut(s)	49, 528, 630, 1101, 1452, 1590
SduI	GDGCHC	8 cut(s)	196, 1026, 1084, 1252, 1636, 1663, 1990, 2134
SexAI	ACCWGGT	1 cut(s)	628
SfaNI	GCATC	2 cut(s)	706, 1270
SfcI	CTRYAG	4 cut(s)	1134, 1692, 1851, 2113
SfiI	GGCCNNNNNGGCC	1 cut(s)	200
Sfr274I	CTCGAG	2 cut(s)	1745, 1842
SfuI	TTCGAA	1 cut(s)	2343
SinI	GGWCC	4 cut(s)	137, 626, 1205, 2388
SlaI	CTCGAG	2 cut(s)	1745, 1842
SmiMI	CAYNNNNRTG	2 cut(s)	1187, 2262
SmlI	CTYRAG	6 cut(s)	400, 1031, 1745, 1842, 2273, 2414
SmoI	CTYRAG	6 cut(s)	400, 1031, 1745, 1842, 2273, 2414
SphI	GCATGC	1 cut(s)	1784
SseBI	AGGCCT	3 cut(s)	203, 1420, 1450
SsiI	CCGC	8 cut(s)	80, 116, 428, 597, 850, 888, 1688, 2378
SspI	AATATT	3 cut(s)	679, 1263, 1512
SspMI	CTAG	3 cut(s)	869, 1121, 1254
SstI	GAGCTC	1 cut(s)	1084
StuI	AGGCCT	3 cut(s)	203, 1420, 1450
StyD4I	CCNGG	6 cut(s)	47, 526, 628, 1099, 1450, 1588
StyI	CCWWGG	3 cut(s)	303, 622, 1902
TaaI	ACNGT	5 cut(s)	743, 1209, 1759, 2185, 2368
TaqI	TCGA	9 cut(s)	34, 144, 260, 1291, 1456, 1746, 1843, 1979, 2343
TatI	WGTACW	2 cut(s)	660, 1340
TauI	GCSGC	1 cut(s)	1691
TfiI	GAWTC	7 cut(s)	347, 359, 692, 906, 1036, 1142, 2090
Tru1I	TTAA	6 cut(s)	419, 507, 972, 1445, 1494, 2331
Tru9I	TTAA	6 cut(s)	419, 507, 972, 1445, 1494, 2331
TscAI	CASTG	4 cut(s)	1111, 1668, 2056, 2373
TseFI	GTSAC	6 cut(s)	1502, 1666, 1828, 2008, 2051, 2311
TseI	GCWGC	8 cut(s)	179, 1577, 1643, 1675, 1853, 1909, 1949, 2118
Tsp45I	GTSAC	6 cut(s)	1502, 1666, 1828, 2008, 2051, 2311
TspDTI	ATGAA	7 cut(s)	372, 1008, 1171, 1563, 1641, 1899, 2082
TspGWI	ACGGA	1 cut(s)	16
TspRI	CASTG	4 cut(s)	1111, 1668, 2056, 2373
Van91I	CCANNNNNTGG	1 cut(s)	1106
VneI	GTGCAC	2 cut(s)	1022, 1659
VpaK11BI	GGWCC	4 cut(s)	137, 626, 1205, 2388
XagI	CCTNNNNNAGG	2 cut(s)	1444, 2273
XapI	RAATTY	5 cut(s)	52, 318, 415, 1016, 1068
XceI	RCATGY	2 cut(s)	1784, 1811
XhoI	CTCGAG	2 cut(s)	1745, 1842
XmnI	GAANNNNTTC	1 cut(s)	2253
XspI	CTAG	3 cut(s)	869, 1121, 1254
ZrmI	AGTACT	1 cut(s)	1342

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.