RLG00000024582

ATP-dependent zinc metalloprotease FTSH 10, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002584
Physical Location & Seq
Reverse (-)
37238865 .. 37243399
4535 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024582

Sequence Viewer

Length: 2448 bp
ATGATTTTCTCCAGCGGTGGACGCCTCCTCTGTCGCTCGGCTCGTTCCGCGTTCCAAAAGAGCGTAATTTGGGTTAATTATAATGGAAGAGCTGCGCTTAGGCACGAACCGCCGACGCCGCTTGGCGGCAATGCGGGCATTTCCGGCGGGTTAGGGCTTCTGAGGGGGTATTTAACTTATAGTGGAGCTGGGAAGCAGCTCGTTTCGAATACCTACATGTCTAATTTGAAATCGGCTCTTGCAAACCCTAGGATTCGTCGCCTGTTCTCCAGCGAAAGGCCTGGGAAGAAAAATTACGAGAATTATTATCCGAAAGATAAGAAGGAAATTCCGAAAGGGAATGATCAGAAATCCGGGTCGAAAGAAGGTGCTGGTGATCAGGGGAATACCCAGGGAAATTTTTCAAAGCAGATTCAAGATTTAATCACTCCTTTATTGTTCATGGGATTTATTTTCATATCGTTATTCCTTACTCCTCAGAAACAGAAGCAGATAAGTTTCCAAGAATTTAAAAACAAGCTACTTGAACCTGGGTTGGTGGATCACATTGAAGTGGCAAATAAATCAGTTGCAAAAGTGTTTGTGAGAAGCTCCCCACGCACTCAAGATGTGAGTAGTGATGATTTTGTCAAGGGACCTGAAAACAGTTCTCCTTCCGGAGGAAATTTGAGCCAGTACAAGTACTATTTTAACATTGGGAGTGTTGACTCTTTTGAGGAAAAATTGGAGGAAGCCCAAGAAGCTTTAGGGATTGACCGTCATGATTTTGTTCCTGTAATTTATGTGGTGCAAATCAATTGGTTCCAAGAGCTGATGAAGTATGCGCCAACAATGTTGCTTTTGGGAGCTCTTTGGTTCATGGGGCGAAAAATGCCTGGTATTGGTGGCACAGGTGGAAAAAGTGGGAGAGGAATATTTAATATAGGGAAAGCACAGATAACAAAAGTGGATAAGAATGCCAAAAACAAGGTTTTCTTTAAAGATGTAGCTGGCTGTGATGAGGCTAAGCAAGAAATTATGGAATTTGTGCACTTTCTCAAGAACCCTAAGAAATATGAGGAATTGGGAGCAAAAATTCCAAAAGGTGCCCTTCTTGTTGGCCCTCCCGGAACTGGAAAAACTCTTATTGCAAAGGCAACTGCAGGTGAATCTGGGGTGCCATTTCTATCTATATCTGGTTCGGATTTTATGGAAATGTTTGTTGGCGTTGGGCCATCAAGGGTTCGGAGTTTATTTCAAGAGGCAAGACAATGTGCACCTAGTATAATATTTATTGATGAGATTGATGCAATTGGCCGGGCAAGAGGGCGTGGAGGCTTTTCAGGTGGCAATGATGAGCGAGAAAGTACTCTCAATCAATTGCTTGTAGAAATGGATGGATTTGGAACAACTGCTGGAGTTGTTGTACTTGCTGGGACCAATAGGCCTGATATTCTGGATAAAGCACTCTTGAGGCCTGGTCGGTTTGACCGTCAAATTACTATTGATAAACCAGACATTAAAGGTCGTAACCAGATTTTTCAGATATATCTGAAAAAGCTCAAATTAGATCGCGAACCATCCCATTACTCAGAGAGACTAGCTGCTCTGACTCCTGGATTCGCTGGAGCAGATATAGCAAATGTCTGCAATGAAGCTGCCTTGATTGCTGCAAGGAATGAGAGTGCAAAGATCACAATGAAACATTTTGAGGCAGCTATCGATCGGGTAATTGGCGGTCTTGAAAAGAAGAACAAGGTAATAAGCAAGCTGGAAAGACGGACTGTTGCTTACCATGAAGCTGGCCATGCTGTTGCTGGTTGGTTCTTAGAACATGCAGAACCTTTACTTAAAGTAACTATTGTTCCACGTGGCACTGCAGCACTGGGGTTCGCTCAGTATGTTCCCAATGAAAATCTTCTTATGACCAAAGAGCAGCTTTTTGATATGACATGCATGACACTTGGTGGTCGAGCTTCTGAAGAGGTTTTGTTGGGGAAGGTTTCAACTGGAGCACAGAACGACTTGGAGAAGGTGACCAAAATGACATATGCCCAAGTTGCAGTATATGGCTTCAGTGACAAAGTGGGCCTTCTTTCTTTTCCTCAAAGGGATGAATTTGAGATGACGAAGCCTTACAGTAGTAAGACTGGTGCAATAATTGACAGTGAAGTTAGAGAATGGGTTGCCAAAGCATATACCCGCACTATTGAGCTGATAGAGGAGCACAAGGAGCACGTAGGACAGATTGCAGAGCTGTTGCTGGAAAAGGAGGTCCTACACCAAGAAGACTTGATTCGAGTCTTGGGAGAGCGCCCATGGAAGTCGGAGGAGCCCACAAATTATGATAGATTCAAGCAAGGGTTCCAAGAAGAAGATAAGGAGGCTAAAGAGACAACCACTGAGGGTAAGGATGTGAAGGATGGTCGGTCACCACCTCTCCAACCAGATGCTGTGCCTGCATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA FtsH_ext Peptidase_M41

Protein Analysis

816

Amino Acids

90.08

Weight (kDa)

8.85

Isoelectric Point (pI)

35.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FtsH_ext PF06480 145 - 253 1.1e-09 FtsH Extracellular
AAA PF00004 364 - 496 2.2e-42 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 525 - 565 3.7e-13 AAA+ lid domain
Peptidase_M41 PF01434 580 - 760 8.9e-65 Peptidase family M41
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 81
AarI CACCTGC 1 cut(s) 1133
Acc36I ACCTGC 1 cut(s) 1133
AccB1I GGYRCC 2 cut(s) 1085, 1156
AccII CGCG 2 cut(s) 50, 1554
AccIII TCCGGA 1 cut(s) 656
AciI CCGC 9 cut(s) 15, 48, 110, 119, 126, 134, 147, 1716, 2182
AclWI GGATC 1 cut(s) 549
AcoI YGGCCR 2 cut(s) 1294, 1783
AcsI RAATTY 7 cut(s) 327, 397, 506, 664, 1022, 1074, 2096
AcuI CTGAAG 2 cut(s) 1980, 2038
AcvI CACGTG 1 cut(s) 1850
AcyI GRCGYC 2 cut(s) 22, 116
AdeI CACNNNGTG 1 cut(s) 1946
AfaI GTAC 4 cut(s) 677, 683, 1348, 1407
AfiI CCNNNNNNNGG 5 cut(s) 125, 276, 659, 881, 1112
AflIII ACRYGT 1 cut(s) 216
AgsI TTSAA 9 cut(s) 229, 405, 416, 527, 551, 1238, 1724, 1986, 2335
AjnI CCWGG 6 cut(s) 280, 390, 529, 874, 1456, 1594
AjuI GAANNNNNNNTTGG 2 cut(s) 1185, 1217
Alw21I GWGCWC 6 cut(s) 850, 1032, 1258, 1996, 2208, 2217
Alw26I GTCTC 2 cut(s) 1570, 2366
Alw44I GTGCAC 2 cut(s) 1028, 1254
AlwI GGATC 1 cut(s) 549
AlwNI CAGNNNCTG 1 cut(s) 2432
Aor13HI TCCGGA 1 cut(s) 656
AoxI GGCC 8 cut(s) 278, 1099, 1211, 1294, 1424, 1454, 1783, 2068
ApaLI GTGCAC 2 cut(s) 1028, 1254
ApeKI GCWGC 8 cut(s) 92, 196, 1583, 1637, 1649, 1694, 1859, 1915
ApoI RAATTY 7 cut(s) 327, 397, 506, 664, 1022, 1074, 2096
Asp700I GAANNNNTTC 2 cut(s) 400, 1896
AspA2I CCTAGG 1 cut(s) 248
AspLEI GCGC 3 cut(s) 97, 826, 2295
AspS9I GGNCC 6 cut(s) 635, 1100, 1211, 1416, 2068, 2254
AsuC2I CCSGG 3 cut(s) 355, 1107, 1298
AsuHPI GGTGA 4 cut(s) 386, 1157, 2026, 2403
AsuII TTCGAA 1 cut(s) 206
AvaII GGWCC 3 cut(s) 635, 1416, 2254
AvrII CCTAGG 1 cut(s) 248
BaeGI GKGCMC 3 cut(s) 1032, 1090, 1258
BalI TGGCCA 1 cut(s) 1785
BanI GGYRCC 2 cut(s) 1085, 1156
BanII GRGCYC 2 cut(s) 850, 2316
BarI GAAGNNNNNNTAC 2 cut(s) 278, 310
BbrPI CACGTG 1 cut(s) 1850
BbsI GAAGAC 1 cut(s) 2274
Bbv12I GWGCWC 6 cut(s) 850, 1032, 1258, 1996, 2208, 2217
BbvI GCAGC 8 cut(s) 79, 208, 1570, 1624, 1636, 1706, 1871, 1927
BccI CCATC 4 cut(s) 1222, 1370, 1567, 2396
BciT130I CCWGG 6 cut(s) 282, 392, 531, 876, 1458, 1596
BclI TGATCA 2 cut(s) 343, 376
BcnI CCSGG 3 cut(s) 355, 1107, 1298
BcoDI GTCTC 2 cut(s) 1570, 2366
BfaI CTAG 3 cut(s) 249, 1260, 1580
BfmI CTRYAG 2 cut(s) 1140, 1857
BfoI RGCGCY 1 cut(s) 2296
BfuAI ACCTGC 1 cut(s) 1133
BlnI CCTAGG 1 cut(s) 248
BlpI GCTNAGC 1 cut(s) 1005
BmcAI AGTACT 2 cut(s) 683, 1348
Bme1390I CCNGG 9 cut(s) 282, 355, 392, 531, 876, 1107, 1298, 1458, 1596
Bme18I GGWCC 3 cut(s) 635, 1416, 2254
BmgT120I GGNCC 6 cut(s) 635, 1100, 1211, 1416, 2068, 2254
BmiI GGNNCC 7 cut(s) 636, 803, 1087, 1158, 1417, 2313, 2345
BmrFI CCNGG 9 cut(s) 282, 355, 392, 531, 876, 1107, 1298, 1458, 1596
BmrI ACTGGG 1 cut(s) 1874
BmsI GCATC 2 cut(s) 1276, 2419
BmuI ACTGGG 1 cut(s) 1874
BpiI GAAGAC 1 cut(s) 2274
BpmI CTGGAG 4 cut(s) 253, 1416, 1626, 2010
Bpu10I CCTNAGC 1 cut(s) 98
Bpu1102I GCTNAGC 1 cut(s) 1005
Bpu14I TTCGAA 1 cut(s) 206
BpuEI CTTGAG 3 cut(s) 588, 1022, 1471
BpuMI CCSGG 3 cut(s) 355, 1107, 1298
Bsa29I ATCGAT 1 cut(s) 1701
BsaAI YACGTR 2 cut(s) 1850, 2218
BsaHI GRCGYC 2 cut(s) 22, 116
BsaJI CCNNGG 6 cut(s) 248, 281, 390, 391, 530, 2297
BsaWI WCCGGW 1 cut(s) 656
BsaXI ACNNNNNCTCC 4 cut(s) 1389, 1419, 2403, 2433
Bsc4I CCNNNNNNNGG 5 cut(s) 125, 276, 659, 881, 1112
Bse1I ACTGG 5 cut(s) 673, 1117, 1869, 1993, 2134
Bse3DI GCAATG 3 cut(s) 136, 1336, 1636
BseAI TCCGGA 1 cut(s) 656
BseBI CCWGG 6 cut(s) 282, 392, 531, 876, 1458, 1596
BseCI ATCGAT 1 cut(s) 1701
BseDI CCNNGG 6 cut(s) 248, 281, 390, 391, 530, 2297
BseGI GGATG 5 cut(s) 1381, 1559, 2098, 2398, 2407
BseLI CCNNNNNNNGG 5 cut(s) 125, 276, 659, 881, 1112
BseMI GCAATG 3 cut(s) 136, 1336, 1636
BseMII CTCAG 5 cut(s) 152, 491, 1584, 1889, 2373
BseNI ACTGG 5 cut(s) 673, 1117, 1869, 1993, 2134
BseRI GAGGAG 4 cut(s) 17, 465, 2216, 2324
BseSI GKGCMC 3 cut(s) 1032, 1090, 1258
BseXI GCAGC 8 cut(s) 79, 208, 1570, 1624, 1636, 1706, 1871, 1927
BseYI CCCAGC 2 cut(s) 188, 1412
Bsh1236I CGCG 2 cut(s) 50, 1554
Bsh1285I CGRYCG 1 cut(s) 1705
BshFI GGCC 8 cut(s) 280, 1101, 1213, 1296, 1426, 1456, 1785, 2070
BshNI GGYRCC 2 cut(s) 1085, 1156
BshVI ATCGAT 1 cut(s) 1701
BsiEI CGRYCG 1 cut(s) 1705
BsiHKAI GWGCWC 6 cut(s) 850, 1032, 1258, 1996, 2208, 2217
BsiSI CCGG 5 cut(s) 144, 354, 657, 1107, 1297
BslFI GGGAC 2 cut(s) 648, 1429
BslI CCNNNNNNNGG 5 cut(s) 125, 276, 659, 881, 1112
BsmAI GTCTC 2 cut(s) 1570, 2366
BsmFI GGGAC 2 cut(s) 648, 1429
BsmI GAATGC 1 cut(s) 961
BsnI GGCC 8 cut(s) 280, 1101, 1213, 1296, 1426, 1456, 1785, 2070
Bsp119I TTCGAA 1 cut(s) 206
Bsp1286I GDGCHC 8 cut(s) 850, 1032, 1090, 1258, 1996, 2208, 2217, 2316
Bsp13I TCCGGA 1 cut(s) 656
Bsp143I GATC 6 cut(s) 343, 376, 541, 1549, 1671, 1702
Bsp1720I GCTNAGC 1 cut(s) 1005
Bsp19I CCATGG 1 cut(s) 2297
Bsp68I TCGCGA 1 cut(s) 1554
BspACI CCGC 9 cut(s) 15, 48, 110, 119, 126, 134, 147, 1716, 2182
BspANI GGCC 8 cut(s) 280, 1101, 1213, 1296, 1426, 1456, 1785, 2070
BspCNI CTCAG 5 cut(s) 153, 490, 1583, 1888, 2374
BspDI ATCGAT 1 cut(s) 1701
BspEI TCCGGA 1 cut(s) 656
BspFNI CGCG 2 cut(s) 50, 1554
BspHI TCATGA 1 cut(s) 760
BspLI GGNNCC 7 cut(s) 636, 803, 1087, 1158, 1417, 2313, 2345
BspMAI CTGCAG 2 cut(s) 1144, 1861
BspMI ACCTGC 1 cut(s) 1133
BspPI GGATC 1 cut(s) 549
BspQI GCTCTTC 1 cut(s) 82
BspT104I TTCGAA 1 cut(s) 206
BspT107I GGYRCC 2 cut(s) 1085, 1156
BsrDI GCAATG 3 cut(s) 136, 1336, 1636
BsrI ACTGG 5 cut(s) 673, 1117, 1869, 1993, 2134
BssECI CCNNGG 6 cut(s) 248, 281, 390, 391, 530, 2297
BssMI GATC 6 cut(s) 343, 376, 541, 1549, 1671, 1702
BssNI GRCGYC 2 cut(s) 22, 116
BssT1I CCWWGG 2 cut(s) 248, 2297
Bst2UI CCWGG 6 cut(s) 282, 392, 531, 876, 1458, 1596
Bst4CI ACNGT 6 cut(s) 647, 758, 1472, 1765, 2120, 2147
Bst6I CTCTTC 2 cut(s) 82, 1956
BstACI GRCGYC 2 cut(s) 22, 116
BstBAI YACGTR 2 cut(s) 1850, 2218
BstBI TTCGAA 1 cut(s) 206
BstC8I GCNNGC 5 cut(s) 136, 991, 1748, 1783, 2439
BstDEI CTNAG 9 cut(s) 98, 161, 477, 1005, 1047, 1570, 1807, 1875, 2382
BstDSI CCRYGG 1 cut(s) 2297
BstEII GGTNACC 2 cut(s) 2014, 2409
BstENI CCTNNNNNAGG 1 cut(s) 657
BstF5I GGATG 5 cut(s) 1381, 1559, 2098, 2398, 2407
BstFNI CGCG 2 cut(s) 50, 1554
BstH2I RGCGCY 1 cut(s) 2296
BstHHI GCGC 3 cut(s) 97, 826, 2295
BstKTI GATC 6 cut(s) 346, 379, 544, 1552, 1674, 1705
BstMAI GTCTC 2 cut(s) 1570, 2366
BstMBI GATC 6 cut(s) 343, 376, 541, 1549, 1671, 1702
BstMCI CGRYCG 1 cut(s) 1705
BstNI CCWGG 6 cut(s) 282, 392, 531, 876, 1458, 1596
BstNSI RCATGY 3 cut(s) 220, 1817, 1935
BstPI GGTNACC 2 cut(s) 2014, 2409
BstSCI CCNGG 9 cut(s) 280, 353, 390, 529, 874, 1105, 1296, 1456, 1594
BstSFI CTRYAG 2 cut(s) 1140, 1857
BstSLI GKGCMC 3 cut(s) 1032, 1090, 1258
BstUI CGCG 2 cut(s) 50, 1554
BstV1I GCAGC 8 cut(s) 79, 208, 1570, 1624, 1636, 1706, 1871, 1927
BstV2I GAAGAC 1 cut(s) 2274
BstXI CCANNNNNNTGG 1 cut(s) 1781
Bsu15I ATCGAT 1 cut(s) 1701
BsuRI GGCC 8 cut(s) 280, 1101, 1213, 1296, 1426, 1456, 1785, 2070
BsuTUI ATCGAT 1 cut(s) 1701
BtgI CCRYGG 1 cut(s) 2297
BtsCI GGATG 5 cut(s) 1381, 1559, 2098, 2398, 2407
BtsI GCAGTG 1 cut(s) 1854
BtsIMutI CAGTG 5 cut(s) 1854, 1862, 2062, 2152, 2379
BtuMI TCGCGA 1 cut(s) 1554
BveI ACCTGC 1 cut(s) 1133
Cac8I GCNNGC 5 cut(s) 136, 991, 1748, 1783, 2439
CaiI CAGNNNCTG 1 cut(s) 2432
CciI TCATGA 1 cut(s) 760
CfoI GCGC 3 cut(s) 97, 826, 2295
Cfr13I GGNCC 6 cut(s) 635, 1100, 1211, 1416, 2068, 2254
ClaI ATCGAT 1 cut(s) 1701
CseI GACGC 2 cut(s) 30, 124
Csp6I GTAC 4 cut(s) 676, 682, 1347, 1406
CviQI GTAC 4 cut(s) 676, 682, 1347, 1406
DdeI CTNAG 9 cut(s) 98, 161, 477, 1005, 1047, 1570, 1807, 1875, 2382
DpnI GATC 6 cut(s) 345, 378, 543, 1551, 1673, 1704
DpnII GATC 6 cut(s) 343, 376, 541, 1549, 1671, 1702
DraI TTTAAA 2 cut(s) 511, 979
DraIII CACNNNGTG 1 cut(s) 1946
EaeI YGGCCR 2 cut(s) 1294, 1783
Eam1104I CTCTTC 2 cut(s) 82, 1956
EarI CTCTTC 2 cut(s) 82, 1956
Ecl136II GAGCTC 1 cut(s) 848
Eco130I CCWWGG 2 cut(s) 248, 2297
Eco147I AGGCCT 3 cut(s) 280, 1426, 1456
Eco24I GRGCYC 2 cut(s) 850, 2316
Eco47I GGWCC 3 cut(s) 635, 1416, 2254
Eco53kI GAGCTC 1 cut(s) 848
Eco57I CTGAAG 2 cut(s) 1980, 2038
Eco72I CACGTG 1 cut(s) 1850
Eco91I GGTNACC 2 cut(s) 2014, 2409
EcoICRI GAGCTC 1 cut(s) 848
EcoNI CCTNNNNNAGG 1 cut(s) 657
EcoO109I RGGNCCY 2 cut(s) 635, 2254
EcoO65I GGTNACC 2 cut(s) 2014, 2409
EcoRII CCWGG 6 cut(s) 280, 390, 529, 874, 1456, 1594
EcoT14I CCWWGG 2 cut(s) 248, 2297
EcoT22I ATGCAT 1 cut(s) 1937
EcoT38I GRGCYC 2 cut(s) 850, 2316
ErhI CCWWGG 2 cut(s) 248, 2297
FalI AAGNNNNNCTT 8 cut(s) 959, 991, 1074, 1106, 1902, 1934, 2055, 2087
FaqI GGGAC 2 cut(s) 648, 1429
FauI CCCGC 3 cut(s) 127, 140, 2189
FauNDI CATATG 1 cut(s) 2029
FbaI TGATCA 2 cut(s) 343, 376
FokI GGATG 5 cut(s) 1388, 1546, 2105, 2405, 2414
FriOI GRGCYC 2 cut(s) 850, 2316
FspBI CTAG 3 cut(s) 249, 1260, 1580
GlaI GCGC 3 cut(s) 96, 825, 2294
GsaI CCCAGC 2 cut(s) 192, 1416
GsuI CTGGAG 4 cut(s) 253, 1416, 1626, 2010
HaeII RGCGCY 1 cut(s) 2296
HaeIII GGCC 8 cut(s) 280, 1101, 1213, 1296, 1426, 1456, 1785, 2070
HapII CCGG 5 cut(s) 144, 354, 657, 1107, 1297
HgaI GACGC 2 cut(s) 30, 124
HhaI GCGC 3 cut(s) 97, 826, 2295
Hin1I GRCGYC 2 cut(s) 22, 116
Hin6I GCGC 3 cut(s) 95, 824, 2293
HinP1I GCGC 3 cut(s) 95, 824, 2293
HincII GTYRAC 1 cut(s) 706
HindII GTYRAC 1 cut(s) 706
HindIII AAGCTT 1 cut(s) 741
HinfI GANTC 9 cut(s) 253, 412, 707, 1148, 1591, 1599, 2275, 2280, 2331
HpaII CCGG 5 cut(s) 144, 354, 657, 1107, 1297
HphI GGTGA 4 cut(s) 386, 1157, 2026, 2403
Hpy166II GTNNAC 4 cut(s) 20, 706, 1030, 1256
Hpy8I GTNNAC 4 cut(s) 20, 706, 1030, 1256
Hpy99I CGWCG 2 cut(s) 118, 261
HpyAV CCTTC 8 cut(s) 316, 359, 663, 1100, 1972, 2005, 2081, 2392
HpyCH4III ACNGT 6 cut(s) 647, 758, 1472, 1765, 2120, 2147
HpyCH4IV ACGT 2 cut(s) 1849, 2217
HpyF3I CTNAG 9 cut(s) 98, 161, 477, 1005, 1047, 1570, 1807, 1875, 2382
HpySE526I ACGT 2 cut(s) 1849, 2217
Hsp92I GRCGYC 2 cut(s) 22, 116
HspAI GCGC 3 cut(s) 95, 824, 2293
Kpn2I TCCGGA 1 cut(s) 656
Ksp22I TGATCA 2 cut(s) 343, 376
Kzo9I GATC 6 cut(s) 343, 376, 541, 1549, 1671, 1702
LguI GCTCTTC 1 cut(s) 82
LmnI GCTCC 9 cut(s) 185, 596, 845, 1067, 1607, 1991, 2203, 2212, 2311
Lsp1109I GCAGC 8 cut(s) 79, 208, 1570, 1624, 1636, 1706, 1871, 1927
LweI GCATC 2 cut(s) 1276, 2419
MaeI CTAG 3 cut(s) 249, 1260, 1580
MaeII ACGT 2 cut(s) 1849, 2217
MaeIII GTNAC 5 cut(s) 1508, 1834, 2014, 2057, 2409
MalI GATC 6 cut(s) 345, 378, 543, 1551, 1673, 1704
MboI GATC 6 cut(s) 343, 376, 541, 1549, 1671, 1702
MboII GAAGA 8 cut(s) 99, 298, 1741, 1889, 1973, 2279, 2363, 2366
MfeI CAATTG 3 cut(s) 796, 1290, 1358
MhlI GDGCHC 8 cut(s) 850, 1032, 1090, 1258, 1996, 2208, 2217, 2316
MlsI TGGCCA 1 cut(s) 1785
MluNI TGGCCA 1 cut(s) 1785
MlyI GAGTC 3 cut(s) 701, 1585, 2289
MmeI TCCRAC 2 cut(s) 2286, 2446
Mox20I TGGCCA 1 cut(s) 1785
Mph1103I ATGCAT 1 cut(s) 1937
MroI TCCGGA 1 cut(s) 656
MroXI GAANNNNTTC 2 cut(s) 400, 1896
MscI TGGCCA 1 cut(s) 1785
MseI TTAA 9 cut(s) 75, 173, 422, 510, 690, 918, 978, 1500, 1830
MslI CAYNNNNRTG 1 cut(s) 551
Msp20I TGGCCA 1 cut(s) 1785
MspA1I CMGCKG 1 cut(s) 15
MspI CCGG 5 cut(s) 144, 354, 657, 1107, 1297
MspR9I CCNGG 9 cut(s) 282, 355, 392, 531, 876, 1107, 1298, 1458, 1596
MunI CAATTG 3 cut(s) 796, 1290, 1358
Mva1269I GAATGC 1 cut(s) 961
MvaI CCWGG 6 cut(s) 282, 392, 531, 876, 1458, 1596
MvnI CGCG 2 cut(s) 50, 1554
NciI CCSGG 3 cut(s) 355, 1107, 1298
NcoI CCATGG 1 cut(s) 2297
NdeI CATATG 1 cut(s) 2029
NdeII GATC 6 cut(s) 343, 376, 541, 1549, 1671, 1702
NlaIV GGNNCC 7 cut(s) 636, 803, 1087, 1158, 1417, 2313, 2345
NmeAIII GCCGAG 1 cut(s) 17
NmuCI GTSAC 3 cut(s) 2014, 2057, 2409
NruI TCGCGA 1 cut(s) 1554
NsiI ATGCAT 1 cut(s) 1937
NspI RCATGY 3 cut(s) 220, 1817, 1935
NspV TTCGAA 1 cut(s) 206
PagI TCATGA 1 cut(s) 760
PaqCI CACCTGC 1 cut(s) 1133
PasI CCCWGGG 1 cut(s) 391
PceI AGGCCT 3 cut(s) 280, 1426, 1456
PciI ACATGT 1 cut(s) 216
PciSI GCTCTTC 1 cut(s) 82
PcsI WCGNNNNNNNCGW 2 cut(s) 40, 1468
PctI GAATGC 1 cut(s) 961
PdmI GAANNNNTTC 2 cut(s) 400, 1896
PfeI GAWTC 6 cut(s) 253, 412, 1148, 1599, 2275, 2331
PfoI TCCNGGA 2 cut(s) 1105, 1594
Ple19I CGATCG 1 cut(s) 1705
PleI GAGTC 3 cut(s) 701, 1585, 2288
PmaCI CACGTG 1 cut(s) 1850
PmlI CACGTG 1 cut(s) 1850
PpsI GAGTC 3 cut(s) 701, 1585, 2288
Ppu21I YACGTR 2 cut(s) 1850, 2218
PpuMI RGGWCCY 2 cut(s) 635, 2254
PscI ACATGT 1 cut(s) 216
PsiI TTATAA 1 cut(s) 81
Psp124BI GAGCTC 1 cut(s) 850
Psp5II RGGWCCY 2 cut(s) 635, 2254
Psp6I CCWGG 6 cut(s) 280, 390, 529, 874, 1456, 1594
PspCI CACGTG 1 cut(s) 1850
PspEI GGTNACC 2 cut(s) 2014, 2409
PspFI CCCAGC 2 cut(s) 188, 1412
PspGI CCWGG 6 cut(s) 280, 390, 529, 874, 1456, 1594
PspN4I GGNNCC 7 cut(s) 636, 803, 1087, 1158, 1417, 2313, 2345
PspPI GGNCC 6 cut(s) 635, 1100, 1211, 1416, 2068, 2254
PspPPI RGGWCCY 2 cut(s) 635, 2254
PsrI GAACNNNNNNTAC 2 cut(s) 1827, 1859
PstI CTGCAG 2 cut(s) 1144, 1861
PstNI CAGNNNCTG 1 cut(s) 2432
PvuI CGATCG 1 cut(s) 1705
RruI TCGCGA 1 cut(s) 1554
RsaI GTAC 4 cut(s) 677, 683, 1348, 1407
RsaNI GTAC 4 cut(s) 676, 682, 1347, 1406
RseI CAYNNNNRTG 1 cut(s) 551
SacI GAGCTC 1 cut(s) 850
SapI GCTCTTC 1 cut(s) 82
SaqAI TTAA 9 cut(s) 75, 173, 422, 510, 690, 918, 978, 1500, 1830
Sau3AI GATC 6 cut(s) 343, 376, 541, 1549, 1671, 1702
Sau96I GGNCC 6 cut(s) 635, 1100, 1211, 1416, 2068, 2254
ScaI AGTACT 2 cut(s) 683, 1348
SchI GAGTC 3 cut(s) 701, 1585, 2289
ScrFI CCNGG 9 cut(s) 282, 355, 392, 531, 876, 1107, 1298, 1458, 1596
SduI GDGCHC 8 cut(s) 850, 1032, 1090, 1258, 1996, 2208, 2217, 2316
SfaNI GCATC 2 cut(s) 1276, 2419
SfcI CTRYAG 2 cut(s) 1140, 1857
SfuI TTCGAA 1 cut(s) 206
SinI GGWCC 3 cut(s) 635, 1416, 2254
SmiMI CAYNNNNRTG 1 cut(s) 551
SmlI CTYRAG 3 cut(s) 603, 1037, 1450
SmoI CTYRAG 3 cut(s) 603, 1037, 1450
SseBI AGGCCT 3 cut(s) 280, 1426, 1456
SsiI CCGC 9 cut(s) 15, 48, 110, 119, 126, 134, 147, 1716, 2182
SspI AATATT 2 cut(s) 915, 1269
SspMI CTAG 3 cut(s) 249, 1260, 1580
SstI GAGCTC 1 cut(s) 850
StuI AGGCCT 3 cut(s) 280, 1426, 1456
StyD4I CCNGG 9 cut(s) 280, 353, 390, 529, 874, 1105, 1296, 1456, 1594
StyI CCWWGG 2 cut(s) 248, 2297
TaaI ACNGT 6 cut(s) 647, 758, 1472, 1765, 2120, 2147
TaiI ACGT 2 cut(s) 1852, 2220
TaqI TCGA 5 cut(s) 206, 359, 1701, 1951, 2278
TaqII GACCGA 1 cut(s) 2397
TatI WGTACW 4 cut(s) 675, 681, 1346, 1405
TauI GCSGC 2 cut(s) 121, 129
TfiI GAWTC 6 cut(s) 253, 412, 1148, 1599, 2275, 2331
Tru1I TTAA 9 cut(s) 75, 173, 422, 510, 690, 918, 978, 1500, 1830
Tru9I TTAA 9 cut(s) 75, 173, 422, 510, 690, 918, 978, 1500, 1830
TscAI CASTG 5 cut(s) 1861, 1869, 2062, 2152, 2386
TseFI GTSAC 3 cut(s) 2014, 2057, 2409
TseI GCWGC 8 cut(s) 92, 196, 1583, 1637, 1649, 1694, 1859, 1915
Tsp45I GTSAC 3 cut(s) 2014, 2057, 2409
TspDTI ATGAA 9 cut(s) 430, 445, 830, 847, 1647, 1694, 1791, 1905, 2109
TspGWI ACGGA 1 cut(s) 1774
TspRI CASTG 5 cut(s) 1861, 1869, 2062, 2152, 2386
VneI GTGCAC 2 cut(s) 1028, 1254
VpaK11BI GGWCC 3 cut(s) 635, 1416, 2254
XagI CCTNNNNNAGG 1 cut(s) 657
XapI RAATTY 7 cut(s) 327, 397, 506, 664, 1022, 1074, 2096
XceI RCATGY 3 cut(s) 220, 1817, 1935
XcmI CCANNNNNNNNNTGG 1 cut(s) 1793
XmaJI CCTAGG 1 cut(s) 248
XmnI GAANNNNTTC 2 cut(s) 400, 1896
XspI CTAG 3 cut(s) 249, 1260, 1580
ZrmI AGTACT 2 cut(s) 683, 1348
Zsp2I ATGCAT 1 cut(s) 1937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.