Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0002584

Physical Location & Seq

Genomic Coordinates Forward (+)

8076600 .. 8081898

Gene Structure 5299 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1097400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2439 bp

ATGATTTTTTCTCGGATTGGTCGCTCCGTTTCTCGATCTTCTCGCTCCAGTAATTTGTCGAATGGAAGTGGGCGATCGGCGGGTCTGATTGGGAATGGAGGGACTTCTGGAGTTCCGCGTTTTGGTTCGTATCTGGGTCCGGTCGATGGGGAATTAGGGTTTTTGAGGTCGTATTTTGCTGCGTCAATAGCAGCCCACAAAGCATGCGTTTCGGATTTCAGTGGCATTCTTGCAAGCCCTAAACTTTGCCGACACTTTTCGAGTGAAAACCCAAAGAAAAAGAATTATGAGAACTTCTATCCCAAGGAAAAGAAGGAAATTCCGAAAGGTGATGAGCAGAAATCGGAGTCGAAAGACGAATCAAAGACAAATGATCAGGGGAGTTTTCAGGAAACTTTCTTGAGGCAGTTTCAAAATTTAATCACCCCCCTATTGGTCATTGGGCTATTTCTTTCCTCCTTCTCCTTTGGTTCTCCTGATCAGCAACAGATAAGTTTCCAAGAGTTTAAAAACAAACTTCTGGAACCAGGCTTAGTGGATCATATAGTTGTCTCTAATAAATCAGTTGCCAAAGTGTATGTGCGTAACTCACCACGCGGTCAGACAAGTGAGGAAGTTGTCCAAGGACCTGGTACCCCTACTCGTGCAAATGGGGGCCAGTACAAATACTATTTCAATATTGGAAGTGTTGAATCTTTTGAGGAGAAGTTGGAGGATGCCCAGGAAGCATTGGGAATAGACAGTCATGATTTTGTTCCTGTTACATATGCCTCGGAGATGGTTTGGTACCAAGAATTGATGAGATTTGCACCAACACTACTGCTTTTGGGATCCCTCTTGTTTATGGGGCGGAAAATGCAGGGTGGACTAGGTATTGGTGGGCCTGGTGGAAAAAGTGGCCGTGGGATCTTCAATATAGGAAAAGCCCAAGTTACAAAAGTAGATAAAAATGCAAAGAACAAGATCTATTTTAAAGATGTTGCTGGTTGTGATGAAGCAAAGCAGGAAATCATGGAATTTGTGCACTTTCTCAAGAATCCTAAGAAATATGAGGATTTGGGAGCCAAAATTCCCAAAGGAGCTCTATTGGTAGGCCCTCCAGGCACTGGAAAGACCCTTCTAGCAAAAGCAACTGCAGGGGAATCTGGTGTTCCATTTCTGTCTATATCTGGATCAGACTTCATGGAGATGTTCGTCGGTGTTGGACCATCTAGAGTTAGAAACTTGTTTCAGGAAGCAAGGCAATGTGCTCCGAGTATTATATTTATTGATGAGATTGATGCAATTGGTCGAGCAAGGGGGCGTGGGGGATTCTCAGGTTCCAATGATGAGCGTGAGAGTACTCTAAATCAGTTGTTGGTAGAGATGGATGGCTTTGGAACAACTGCTGGAGTAGTGGTTCTTGCTGGCACCAATAGGCCTGATATTTTGGACAAAGCCTTATTAAGGCCTGGTCGATTTGATCGCCAAATCTCTATTGATAGGCCCGATATTAAAGGTCGTGACCAGATATTTCAGATATACTTGAAAAAGATCAAACTTGATCATGAACCATCATATTATTCTCAAAGGCTTGCAGCCCTGACTCCAGGGTTTGCTGGGGCAGACATTGCAAATGTTTGCAATGAAGGTGCTCTAATTGCTGCAAGAAATGAGAGTGCACTGGTCACAATGCAGCATTTTGAGGCGGCTATAGACAGAATTATTGGTGGTTTGGAGAAGAAGAACAGGGTTATAAGCAAGCTCGAGCGCCGTACTGTTGCCTACCATGAATCAGGGCATGCTGTTACCGGCTGGTTCTTAGAACACGCAGAACCGTTGTTGAAAGTGACCATTGTTCCTCGTGGTACTGCAGCACTTGGGTTTGCCCAGTATGTTCCAAATGAAAACCTCCTCATGACCAAGGAGCAGCTTTTTGATATGACCTGCATGACCCTCGGTGGTAGAGCAGCTGAACAGGTTATGTTGGGAAAAATCTCGACAGGAGCCCAAAACGATCTGGAGAAAGTGACAAAGATGACCTACGCCCAAGTTGCAGTCTATGGTTTCAGTGACAAGGTAGGTCTCCTTTCTTTCCCTCAAAGGGAAGAATCATTTGAGATGTCAAAGCCCTACAGCAGCAAGACCGGAGCCCTTATTGATGGTGAAGTGCGCGAATGGGTGGGTAAGGCGTATGCACGCACAGTTGAACTCGTAGAGGAACATAAAGAGCAAATAGCTGAAATTGCAGAACTGTTGCTTGAAAAGGAAGTTCTTCACCAAGATGACCTGATCAAGGTGTTGGGGGAAAGACCATATAAACCGGCTGAAGCGACCAACTACGATAGATTTAAGGACGGTTTTGAAGAGAAAGATGGTGAGAAAACAGTGGAGACACCGCTGGTTGGGTCCGAGGATGACGGCTCATCACCCCTTGAACCTCAGGTTCTGCCTACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004176 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009719 GO:0009725 GO:0009735 GO:0010033 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019538 GO:0031976 GO:0031984 GO:0034357 GO:0042221 GO:0042623 GO:0042651 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0055035 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA FtsH_ext Peptidase_M41

Protein Analysis

813

Amino Acids

89.09

Weight (kDa)

6.28

Isoelectric Point (pI)

32.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FtsH_ext	PF06480	142 - 249	4.2e-12	FtsH Extracellular
AAA	PF00004	362 - 494	7.4e-43	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	524 - 563	1.3e-10	AAA+ lid domain
Peptidase_M41	PF01434	577 - 759	9.7e-65	Peptidase family M41

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002584)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07510 AT2G29080
fragaria_vesca	FvH4_3g36790 FvH4_3g36790 FvH4_3g36790 FvH4_6g13480 FvH4_6g13480 FvH4_6g13480
malus_domestica	MD03G1088300.v1.1 MD03G1088400.v1.1 MD04G1130900.v1.1 MD11G1097400.v1.1 MD12G1144000.v1.1
prunus_persica	Prupe.6G072600_v2.0.a1 Prupe.6G257400_v2.0.a1 Prupe.6G257400_v2.0.a1
pyrus_communis	pycom03g06980 pycom03g07000 pycom04g11920 pycom11g08210 pycom12g13860
rosa_chinensis	RchiOBHm_Chr3g0466351 RchiOBHm_Chr5g0065851
rosa_laevigata	RLG00000024582 RLG00000035821
rosa_multiflora	Rmu_sc0003480.1_g000012 Rmu_sc0042868.1_g000001
rosa_roxburghii	Rroxscaffold_1G00015310 Rroxscaffold_6G00414550
rosa_rugosa	Rorug03G0081200 Rorug05G0374100 Rorug05G0374200 Rorug05G0374300
rosa_samantha	Rh3BG148900 Rh3DG149600 Rh5BG449200 Rh5CG471400 Rh5DG462800
rosa_wichuraiana	Rw3G012050 Rw5G040600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1736
Acc36I	ACCTGC	1 cut(s)	1934
Acc65I	GGTACC	2 cut(s)	632, 786
AccB1I	GGYRCC	3 cut(s)	632, 786, 1409
AccB7I	CCANNNNNTGG	1 cut(s)	1106
AccII	CGCG	3 cut(s)	118, 597, 2154
AciI	CCGC	6 cut(s)	80, 116, 597, 850, 1688, 2378
AclWI	GGATC	5 cut(s)	546, 825, 838, 914, 1180
AcoI	YGGCCR	1 cut(s)	898
AcsI	RAATTY	4 cut(s)	318, 415, 1016, 1068
AcuI	CTGAAG	1 cut(s)	2328
AfaI	GTAC	6 cut(s)	634, 662, 788, 1342, 1756, 1849
AfiI	CCNNNNNNNGG	8 cut(s)	122, 146, 433, 1106, 1446, 2083, 2275, 2384
AjnI	CCWGG	7 cut(s)	526, 628, 720, 883, 1099, 1450, 1588
AloI	GAACNNNNNNTCC	2 cut(s)	1383, 1415
AluBI	AGCT	5 cut(s)	1082, 1744, 1912, 1952, 2219
AluI	AGCT	5 cut(s)	1082, 1744, 1912, 1952, 2219
Alw21I	GWGCWC	5 cut(s)	1026, 1084, 1252, 1636, 1663
Alw26I	GTCTC	3 cut(s)	556, 2069, 2366
Alw44I	GTGCAC	2 cut(s)	1022, 1659
AlwI	GGATC	5 cut(s)	546, 825, 838, 914, 1180
AlwNI	CAGNNNCTG	2 cut(s)	1106, 2428
Ama87I	CYCGRG	1 cut(s)	1745
AoxI	GGCC	7 cut(s)	655, 881, 898, 1093, 1418, 1448, 1484
ApaLI	GTGCAC	2 cut(s)	1022, 1659
ApeKI	GCWGC	9 cut(s)	179, 191, 1577, 1643, 1675, 1853, 1909, 1949, 2118
ApoI	RAATTY	4 cut(s)	318, 415, 1016, 1068
Asp700I	GAANNNNTTC	1 cut(s)	2253
Asp718I	GGTACC	2 cut(s)	632, 786
AspLEI	GCGC	2 cut(s)	1752, 2154
AspS9I	GGNCC	8 cut(s)	137, 626, 655, 881, 1094, 1205, 1485, 2388
AsuHPI	GGTGA	7 cut(s)	341, 415, 582, 2156, 2249, 2369, 2400
AvaI	CYCGRG	1 cut(s)	1745
AvaII	GGWCC	4 cut(s)	137, 626, 1205, 2388
AxyI	CCTNAGG	1 cut(s)	2421
BaeGI	GKGCMC	2 cut(s)	1026, 1663
BamHI	GGATCC	1 cut(s)	830
BanI	GGYRCC	3 cut(s)	632, 786, 1409
BanII	GRGCYC	3 cut(s)	1084, 1990, 2134
BauI	CACGAG	2 cut(s)	642, 1842
Bbv12I	GWGCWC	5 cut(s)	1026, 1084, 1252, 1636, 1663
BbvI	GCAGC	9 cut(s)	166, 203, 1589, 1630, 1687, 1865, 1921, 1961, 2130
BccI	CCATC	8 cut(s)	140, 772, 1216, 1360, 1364, 1561, 2135, 2348
BceAI	ACGGC	3 cut(s)	885, 1737, 2416
BcgI	CGANNNNNNTGC	4 cut(s)	192, 226, 1918, 1952
BciT130I	CCWGG	7 cut(s)	528, 630, 722, 885, 1101, 1452, 1590
BclI	TGATCA	4 cut(s)	373, 478, 1543, 2271
BcoDI	GTCTC	3 cut(s)	556, 2069, 2366
BfaI	CTAG	3 cut(s)	869, 1121, 1212
BfmI	CTRYAG	4 cut(s)	1134, 1692, 1851, 2113
BfoI	RGCGCY	1 cut(s)	1753
BfuAI	ACCTGC	1 cut(s)	1934
BglI	GCCNNNNNGGC	1 cut(s)	1101
BglII	AGATCT	1 cut(s)	963
BmcAI	AGTACT	1 cut(s)	1342
Bme1390I	CCNGG	7 cut(s)	528, 630, 722, 885, 1101, 1452, 1590
Bme18I	GGWCC	4 cut(s)	137, 626, 1205, 2388
BmeT110I	CYCGRG	1 cut(s)	1745
BmgT120I	GGNCC	8 cut(s)	137, 626, 655, 881, 1094, 1205, 1485, 2388
BmrFI	CCNGG	7 cut(s)	528, 630, 722, 885, 1101, 1452, 1590
BmrI	ACTGGG	1 cut(s)	1864
BmsI	GCATC	2 cut(s)	706, 1270
BmuI	ACTGGG	1 cut(s)	1864
BpmI	CTGGAG	6 cut(s)	31, 129, 1083, 1410, 1572, 2021
BpuEI	CTTGAG	2 cut(s)	421, 1016
BsaI	GGTCTC	1 cut(s)	2069
BsaJI	CCNNGG	9 cut(s)	303, 622, 720, 771, 901, 1589, 1902, 1936, 2391
BsaWI	WCCGGW	2 cut(s)	139, 2126
BsaXI	ACNNNNNCTCC	2 cut(s)	1383, 1413
Bsc4I	CCNNNNNNNGG	8 cut(s)	122, 146, 433, 1106, 1446, 2083, 2275, 2384
Bse118I	RCCGGY	2 cut(s)	1790, 2302
Bse1I	ACTGG	5 cut(s)	48, 658, 1111, 1668, 1870
Bse21I	CCTNAGG	1 cut(s)	2421
Bse3DI	GCAATG	3 cut(s)	1250, 1608, 1630
BseBI	CCWGG	7 cut(s)	528, 630, 722, 885, 1101, 1452, 1590
BseDI	CCNNGG	9 cut(s)	303, 622, 720, 771, 901, 1589, 1902, 1936, 2391
BseGI	GGATG	3 cut(s)	721, 1375, 2401
BseLI	CCNNNNNNNGG	8 cut(s)	122, 146, 433, 1106, 1446, 2083, 2275, 2384
BseMI	GCAATG	3 cut(s)	1250, 1608, 1630
BseMII	CTCAG	2 cut(s)	1329, 2435
BseNI	ACTGG	5 cut(s)	48, 658, 1111, 1668, 1870
BseRI	GAGGAG	2 cut(s)	716, 1883
BseSI	GKGCMC	2 cut(s)	1026, 1663
BseXI	GCAGC	9 cut(s)	166, 203, 1589, 1630, 1687, 1865, 1921, 1961, 2130
BseYI	CCCAGC	1 cut(s)	1598
Bsh1236I	CGCG	3 cut(s)	118, 597, 2154
Bsh1285I	CGRYCG	2 cut(s)	77, 144
BshFI	GGCC	7 cut(s)	657, 883, 900, 1095, 1420, 1450, 1486
BshNI	GGYRCC	3 cut(s)	632, 786, 1409
BsiEI	CGRYCG	2 cut(s)	77, 144
BsiHKAI	GWGCWC	5 cut(s)	1026, 1084, 1252, 1636, 1663
BsiHKCI	CYCGRG	1 cut(s)	1745
BsiSI	CCGG	4 cut(s)	140, 1791, 2127, 2303
BslFI	GGGAC	1 cut(s)	115
BslI	CCNNNNNNNGG	8 cut(s)	122, 146, 433, 1106, 1446, 2083, 2275, 2384
BsmAI	GTCTC	3 cut(s)	556, 2069, 2366
BsmFI	GGGAC	1 cut(s)	115
BsmI	GAATGC	1 cut(s)	225
BsnI	GGCC	7 cut(s)	657, 883, 900, 1095, 1420, 1450, 1486
Bso31I	GGTCTC	1 cut(s)	2069
BsoBI	CYCGRG	1 cut(s)	1745
Bsp1286I	GDGCHC	7 cut(s)	1026, 1084, 1252, 1636, 1663, 1990, 2134
BspACI	CCGC	6 cut(s)	80, 116, 597, 850, 1688, 2378
BspANI	GGCC	7 cut(s)	657, 883, 900, 1095, 1420, 1450, 1486
BspCNI	CTCAG	2 cut(s)	1328, 2434
BspFNI	CGCG	3 cut(s)	118, 597, 2154
BspHI	TCATGA	3 cut(s)	745, 1546, 1896
BspMAI	CTGCAG	2 cut(s)	1138, 1855
BspMI	ACCTGC	1 cut(s)	1934
BspPI	GGATC	5 cut(s)	546, 825, 838, 914, 1180
BspT107I	GGYRCC	3 cut(s)	632, 786, 1409
BspTNI	GGTCTC	1 cut(s)	2069
BsrDI	GCAATG	3 cut(s)	1250, 1608, 1630
BsrFI	RCCGGY	2 cut(s)	1790, 2302
BsrI	ACTGG	5 cut(s)	48, 658, 1111, 1668, 1870
BssAI	RCCGGY	2 cut(s)	1790, 2302
BssECI	CCNNGG	9 cut(s)	303, 622, 720, 771, 901, 1589, 1902, 1936, 2391
BssSI	CACGAG	2 cut(s)	642, 1842
BssT1I	CCWWGG	3 cut(s)	303, 622, 1902
Bst2BI	CACGAG	2 cut(s)	642, 1842
Bst2UI	CCWGG	7 cut(s)	528, 630, 722, 885, 1101, 1452, 1590
Bst4CI	ACNGT	7 cut(s)	743, 1759, 1818, 2185, 2235, 2339, 2368
Bst6I	CTCTTC	1 cut(s)	2340
BstAPI	GCANNNNNTGC	1 cut(s)	1610
BstC8I	GCNNGC	7 cut(s)	205, 235, 1408, 1575, 1742, 1782, 2179
BstDEI	CTNAG	6 cut(s)	532, 1041, 1315, 1801, 2421, 2436
BstDSI	CCRYGG	1 cut(s)	901
BstENI	CCTNNNNNAGG	2 cut(s)	1444, 2273
BstF5I	GGATG	3 cut(s)	721, 1375, 2401
BstFNI	CGCG	3 cut(s)	118, 597, 2154
BstH2I	RGCGCY	1 cut(s)	1753
BstHHI	GCGC	2 cut(s)	1752, 2154
BstMAI	GTCTC	3 cut(s)	556, 2069, 2366
BstMCI	CGRYCG	2 cut(s)	77, 144
BstMWI	GCNNNNNNNGC	9 cut(s)	188, 200, 725, 856, 1101, 1610, 1640, 2033, 2225
BstNI	CCWGG	7 cut(s)	528, 630, 722, 885, 1101, 1452, 1590
BstNSI	RCATGY	2 cut(s)	207, 1784
BstSCI	CCNGG	7 cut(s)	526, 628, 720, 883, 1099, 1450, 1588
BstSFI	CTRYAG	4 cut(s)	1134, 1692, 1851, 2113
BstSLI	GKGCMC	2 cut(s)	1026, 1663
BstUI	CGCG	3 cut(s)	118, 597, 2154
BstV1I	GCAGC	9 cut(s)	166, 203, 1589, 1630, 1687, 1865, 1921, 1961, 2130
BstX2I	RGATCY	3 cut(s)	830, 906, 963
BstXI	CCANNNNNNTGG	1 cut(s)	629
BstYI	RGATCY	3 cut(s)	830, 906, 963
Bsu36I	CCTNAGG	1 cut(s)	2421
BsuRI	GGCC	7 cut(s)	657, 883, 900, 1095, 1420, 1450, 1486
BtgI	CCRYGG	1 cut(s)	901
BtsCI	GGATG	3 cut(s)	721, 1375, 2401
BtsIMutI	CAGTG	5 cut(s)	226, 1104, 1661, 2056, 2373
BveI	ACCTGC	1 cut(s)	1934
Cac8I	GCNNGC	7 cut(s)	205, 235, 1408, 1575, 1742, 1782, 2179
CaiI	CAGNNNCTG	2 cut(s)	1106, 2428
CciI	TCATGA	3 cut(s)	745, 1546, 1896
CfoI	GCGC	2 cut(s)	1752, 2154
Cfr10I	RCCGGY	2 cut(s)	1790, 2302
Cfr13I	GGNCC	8 cut(s)	137, 626, 655, 881, 1094, 1205, 1485, 2388
CseI	GACGC	1 cut(s)	171
CsiI	ACCWGGT	1 cut(s)	628
Csp6I	GTAC	6 cut(s)	633, 661, 787, 1341, 1755, 1848
CviAII	CATG	9 cut(s)	204, 746, 1012, 1183, 1547, 1769, 1781, 1897, 1930
CviQI	GTAC	6 cut(s)	633, 661, 787, 1341, 1755, 1848
DdeI	CTNAG	6 cut(s)	532, 1041, 1315, 1801, 2421, 2436
DraI	TTTAAA	2 cut(s)	508, 973
EaeI	YGGCCR	1 cut(s)	898
Eam1104I	CTCTTC	1 cut(s)	2340
EarI	CTCTTC	1 cut(s)	2340
EciI	GGCGGA	1 cut(s)	865
Ecl136II	GAGCTC	1 cut(s)	1082
Eco130I	CCWWGG	3 cut(s)	303, 622, 1902
Eco147I	AGGCCT	2 cut(s)	1420, 1450
Eco24I	GRGCYC	3 cut(s)	1084, 1990, 2134
Eco31I	GGTCTC	1 cut(s)	2069
Eco47I	GGWCC	4 cut(s)	137, 626, 1205, 2388
Eco53kI	GAGCTC	1 cut(s)	1082
Eco57I	CTGAAG	1 cut(s)	2328
Eco81I	CCTNAGG	1 cut(s)	2421
Eco88I	CYCGRG	1 cut(s)	1745
EcoICRI	GAGCTC	1 cut(s)	1082
EcoNI	CCTNNNNNAGG	2 cut(s)	1444, 2273
EcoO109I	RGGNCCY	2 cut(s)	626, 1094
EcoRII	CCWGG	7 cut(s)	526, 628, 720, 883, 1099, 1450, 1588
EcoT14I	CCWWGG	3 cut(s)	303, 622, 1902
EcoT38I	GRGCYC	3 cut(s)	1084, 1990, 2134
ErhI	CCWWGG	3 cut(s)	303, 622, 1902
FaeI	CATG	9 cut(s)	207, 749, 1015, 1186, 1550, 1772, 1784, 1900, 1933
FaqI	GGGAC	1 cut(s)	115
FatI	CATG	9 cut(s)	203, 745, 1011, 1182, 1546, 1768, 1780, 1896, 1929
FauI	CCCGC	1 cut(s)	73
FauNDI	CATATG	1 cut(s)	766
FbaI	TGATCA	4 cut(s)	373, 478, 1543, 2271
FokI	GGATG	3 cut(s)	728, 1382, 2408
FriOI	GRGCYC	3 cut(s)	1084, 1990, 2134
FspBI	CTAG	3 cut(s)	869, 1121, 1212
GlaI	GCGC	2 cut(s)	1751, 2153
GsaI	CCCAGC	1 cut(s)	1602
GsuI	CTGGAG	6 cut(s)	31, 129, 1083, 1410, 1572, 2021
HaeII	RGCGCY	1 cut(s)	1753
HaeIII	GGCC	7 cut(s)	657, 883, 900, 1095, 1420, 1450, 1486
HapII	CCGG	4 cut(s)	140, 1791, 2127, 2303
HgaI	GACGC	1 cut(s)	171
HhaI	GCGC	2 cut(s)	1752, 2154
Hin1II	CATG	9 cut(s)	207, 749, 1015, 1186, 1550, 1772, 1784, 1900, 1933
Hin6I	GCGC	2 cut(s)	1750, 2152
HinP1I	GCGC	2 cut(s)	1750, 2152
HinfI	GANTC	9 cut(s)	347, 359, 692, 1036, 1142, 1311, 1585, 1772, 2090
HpaII	CCGG	4 cut(s)	140, 1791, 2127, 2303
HphI	GGTGA	7 cut(s)	341, 415, 582, 2156, 2249, 2369, 2400
Hpy166II	GTNNAC	3 cut(s)	866, 1024, 1661
Hpy8I	GTNNAC	3 cut(s)	866, 1024, 1661
Hpy99I	CGWCG	1 cut(s)	1199
HpyAV	CCTTC	4 cut(s)	307, 469, 1127, 1622
HpyCH4III	ACNGT	7 cut(s)	743, 1759, 1818, 2185, 2235, 2339, 2368
HpyF10VI	GCNNNNNNNGC	9 cut(s)	188, 200, 725, 856, 1101, 1610, 1640, 2033, 2225
HpyF3I	CTNAG	6 cut(s)	532, 1041, 1315, 1801, 2421, 2436
Hsp92II	CATG	9 cut(s)	207, 749, 1015, 1186, 1550, 1772, 1784, 1900, 1933
HspAI	GCGC	2 cut(s)	1750, 2152
KpnI	GGTACC	2 cut(s)	636, 790
Ksp22I	TGATCA	4 cut(s)	373, 478, 1543, 2271
LmnI	GCTCC	8 cut(s)	29, 50, 1061, 1079, 1255, 1906, 1985, 2129
Lsp1109I	GCAGC	9 cut(s)	166, 203, 1589, 1630, 1687, 1865, 1921, 1961, 2130
LweI	GCATC	2 cut(s)	706, 1270
MabI	ACCWGGT	1 cut(s)	628
MaeI	CTAG	3 cut(s)	869, 1121, 1212
MaeIII	GTNAC	9 cut(s)	584, 760, 931, 1502, 1666, 1786, 1828, 2008, 2051
MboII	GAAGA	7 cut(s)	30, 901, 1732, 1735, 2099, 2246, 2357
MfeI	CAATTG	1 cut(s)	1284
MflI	RGATCY	3 cut(s)	830, 906, 963
MhlI	GDGCHC	7 cut(s)	1026, 1084, 1252, 1636, 1663, 1990, 2134
MlyI	GAGTC	2 cut(s)	356, 1579
MmeI	TCCRAC	2 cut(s)	690, 1183
MroXI	GAANNNNTTC	1 cut(s)	2253
MseI	TTAA	6 cut(s)	419, 507, 972, 1445, 1494, 2331
MslI	CAYNNNNRTG	2 cut(s)	1187, 2262
MspA1I	CMGCKG	2 cut(s)	1952, 2380
MspI	CCGG	4 cut(s)	140, 1791, 2127, 2303
MspR9I	CCNGG	7 cut(s)	528, 630, 722, 885, 1101, 1452, 1590
MunI	CAATTG	1 cut(s)	1284
Mva1269I	GAATGC	1 cut(s)	225
MvaI	CCWGG	7 cut(s)	528, 630, 722, 885, 1101, 1452, 1590
MvnI	CGCG	3 cut(s)	118, 597, 2154
MwoI	GCNNNNNNNGC	9 cut(s)	188, 200, 725, 856, 1101, 1610, 1640, 2033, 2225
NdeI	CATATG	1 cut(s)	766
NlaIII	CATG	9 cut(s)	207, 749, 1015, 1186, 1550, 1772, 1784, 1900, 1933
NmuCI	GTSAC	5 cut(s)	1502, 1666, 1828, 2008, 2051
NspI	RCATGY	2 cut(s)	207, 1784
PaeI	GCATGC	2 cut(s)	207, 1784
PaeR7I	CTCGAG	1 cut(s)	1745
PagI	TCATGA	3 cut(s)	745, 1546, 1896
PceI	AGGCCT	2 cut(s)	1420, 1450
PctI	GAATGC	1 cut(s)	225
PdmI	GAANNNNTTC	1 cut(s)	2253
PfeI	GAWTC	7 cut(s)	359, 692, 1036, 1142, 1311, 1772, 2090
PflMI	CCANNNNNTGG	1 cut(s)	1106
Ple19I	CGATCG	1 cut(s)	77
PleI	GAGTC	2 cut(s)	355, 1579
PpsI	GAGTC	2 cut(s)	355, 1579
PpuMI	RGGWCCY	1 cut(s)	626
PsiI	TTATAA	1 cut(s)	1736
Psp124BI	GAGCTC	1 cut(s)	1084
Psp5II	RGGWCCY	1 cut(s)	626
Psp6I	CCWGG	7 cut(s)	526, 628, 720, 883, 1099, 1450, 1588
PspFI	CCCAGC	1 cut(s)	1598
PspGI	CCWGG	7 cut(s)	526, 628, 720, 883, 1099, 1450, 1588
PspPI	GGNCC	8 cut(s)	137, 626, 655, 881, 1094, 1205, 1485, 2388
PspPPI	RGGWCCY	1 cut(s)	626
PspXI	VCTCGAGB	1 cut(s)	1745
PstI	CTGCAG	2 cut(s)	1138, 1855
PstNI	CAGNNNCTG	2 cut(s)	1106, 2428
PsuI	RGATCY	3 cut(s)	830, 906, 963
PvuI	CGATCG	1 cut(s)	77
PvuII	CAGCTG	1 cut(s)	1952
RsaI	GTAC	6 cut(s)	634, 662, 788, 1342, 1756, 1849
RsaNI	GTAC	6 cut(s)	633, 661, 787, 1341, 1755, 1848
RseI	CAYNNNNRTG	2 cut(s)	1187, 2262
SacI	GAGCTC	1 cut(s)	1084
SaqAI	TTAA	6 cut(s)	419, 507, 972, 1445, 1494, 2331
Sau96I	GGNCC	8 cut(s)	137, 626, 655, 881, 1094, 1205, 1485, 2388
ScaI	AGTACT	1 cut(s)	1342
SchI	GAGTC	2 cut(s)	356, 1579
ScrFI	CCNGG	7 cut(s)	528, 630, 722, 885, 1101, 1452, 1590
SduI	GDGCHC	7 cut(s)	1026, 1084, 1252, 1636, 1663, 1990, 2134
SexAI	ACCWGGT	1 cut(s)	628
SfaNI	GCATC	2 cut(s)	706, 1270
SfcI	CTRYAG	4 cut(s)	1134, 1692, 1851, 2113
Sfr274I	CTCGAG	1 cut(s)	1745
SinI	GGWCC	4 cut(s)	137, 626, 1205, 2388
SlaI	CTCGAG	1 cut(s)	1745
SmiMI	CAYNNNNRTG	2 cut(s)	1187, 2262
SmlI	CTYRAG	3 cut(s)	400, 1031, 1745
SmoI	CTYRAG	3 cut(s)	400, 1031, 1745
SphI	GCATGC	2 cut(s)	207, 1784
SseBI	AGGCCT	2 cut(s)	1420, 1450
SsiI	CCGC	6 cut(s)	80, 116, 597, 850, 1688, 2378
SspI	AATATT	1 cut(s)	679
SspMI	CTAG	3 cut(s)	869, 1121, 1212
SstI	GAGCTC	1 cut(s)	1084
StuI	AGGCCT	2 cut(s)	1420, 1450
StyD4I	CCNGG	7 cut(s)	526, 628, 720, 883, 1099, 1450, 1588
StyI	CCWWGG	3 cut(s)	303, 622, 1902
TaaI	ACNGT	7 cut(s)	743, 1759, 1818, 2185, 2235, 2339, 2368
TaqI	TCGA	9 cut(s)	34, 59, 144, 260, 350, 1291, 1456, 1746, 1979
TatI	WGTACW	2 cut(s)	660, 1340
TauI	GCSGC	1 cut(s)	1691
TfiI	GAWTC	7 cut(s)	359, 692, 1036, 1142, 1311, 1772, 2090
Tru1I	TTAA	6 cut(s)	419, 507, 972, 1445, 1494, 2331
Tru9I	TTAA	6 cut(s)	419, 507, 972, 1445, 1494, 2331
TscAI	CASTG	5 cut(s)	226, 1111, 1668, 2056, 2373
TseFI	GTSAC	5 cut(s)	1502, 1666, 1828, 2008, 2051
TseI	GCWGC	9 cut(s)	179, 191, 1577, 1643, 1675, 1853, 1909, 1949, 2118
Tsp45I	GTSAC	5 cut(s)	1502, 1666, 1828, 2008, 2051
TspDTI	ATGAA	6 cut(s)	1008, 1171, 1563, 1641, 1785, 1899
TspGWI	ACGGA	1 cut(s)	16
TspRI	CASTG	5 cut(s)	226, 1111, 1668, 2056, 2373
Van91I	CCANNNNNTGG	1 cut(s)	1106
VneI	GTGCAC	2 cut(s)	1022, 1659
VpaK11BI	GGWCC	4 cut(s)	137, 626, 1205, 2388
XagI	CCTNNNNNAGG	2 cut(s)	1444, 2273
XapI	RAATTY	4 cut(s)	318, 415, 1016, 1068
XbaI	TCTAGA	1 cut(s)	1211
XceI	RCATGY	2 cut(s)	207, 1784
XhoI	CTCGAG	1 cut(s)	1745
XmnI	GAANNNNTTC	1 cut(s)	2253
XspI	CTAG	3 cut(s)	869, 1121, 1212
ZrmI	AGTACT	1 cut(s)	1342

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.