pycom12g13860

ATP-dependent zinc metalloprotease FTSH 10, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002584
Physical Location & Seq
Reverse (-)
16345855 .. 16347133
1279 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g13860.1

Sequence Viewer

Length: 723 bp
ATGGAGCTGGGAATCCATAGAATTTGGAGTCTGTTCTCGGAATCCATAGAATTCGTCGCTTGTTCTCCAGCCAAGGCCCCGGGAAGAAAAATTACGAAAATTATTATCCGAAAAATAAGAAGGAGATTCCGAAAGGGGGCGAACAGAAATCAGCGTCCAAAGAGGGATCAAGTGCAGGTGGTCAGGGGAATACTCAGGGACAGTTTTCGAAGCAGATTCAAGATTTTATTGCTCCTAATGTTCTTTGGCTTTATTGTCACATCATTACTCCTTAATCCTCATCAAACGAAGGAGATAAGTTTCCAAGAATTAAAAAATAAGCTACTTGAACTTGGGTTGGTCGATCACATTGAAGTTGCAAACAAATCAGTTGCAAAAGTGTACGTGAGGAGCTCCGCACGTGAAAAAGTTCATAGTGATGATTCTGTCCGTGATACTGTTAATGGTTCTCCTTCCGAAGGAAATGTAACCCAGTTTAAATATTATTTTAACATTGGGAGTGTTCAATCTTTTGAAGAAAAGTTGGAGGAAGCCCAAGAAGCTCTAGGGATTGACCGTCATGATTTTGTTCCTGTAATTTATGTATCCCAAGTAATGTGGTCCAAAGAGTTGTTAAGGTACGGGCCAACAGTGTTGCTTCTGGGAACCCTTTGGTTCATGAGCAGAAAAATGCCTAGCATTTTGGTCCAGGTGGAAAAAGCGGCCAAGGAATATTCAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA FtsH_ext Peptidase_M41

Protein Analysis

241

Amino Acids

27.72

Weight (kDa)

9.96

Isoelectric Point (pI)

52.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FtsH_ext PF06480 76 - 186 3.5e-09 FtsH Extracellular
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 166
Acc36I ACCTGC 1 cut(s) 166
AciI CCGC 2 cut(s) 396, 701
AclWI GGATC 1 cut(s) 174
AcoI YGGCCR 1 cut(s) 702
AcsI RAATTY 2 cut(s) 21, 50
AcvI CACGTG 1 cut(s) 401
AfaI GTAC 2 cut(s) 383, 620
AfiI CCNNNNNNNGG 2 cut(s) 136, 458
AgsI TTSAA 6 cut(s) 220, 329, 353, 506, 515, 717
AjnI CCWGG 1 cut(s) 687
AluBI AGCT 4 cut(s) 7, 322, 393, 542
AluI AGCT 4 cut(s) 7, 322, 393, 542
Alw21I GWGCWC 1 cut(s) 395
AlwI GGATC 1 cut(s) 174
Ama87I CYCGRG 1 cut(s) 79
AoxI GGCC 3 cut(s) 75, 623, 702
ApoI RAATTY 2 cut(s) 21, 50
Asp700I GAANNNNTTC 1 cut(s) 408
AspS9I GGNCC 4 cut(s) 76, 600, 623, 685
AsuC2I CCSGG 2 cut(s) 80, 81
AsuII TTCGAA 1 cut(s) 208
AvaI CYCGRG 1 cut(s) 79
AvaII GGWCC 2 cut(s) 600, 685
BanII GRGCYC 1 cut(s) 395
BarI GAAGNNNNNNTAC 2 cut(s) 76, 108
BbrPI CACGTG 1 cut(s) 401
Bbv12I GWGCWC 1 cut(s) 395
BciT130I CCWGG 1 cut(s) 689
BciVI GTATCC 1 cut(s) 595
BcnI CCSGG 2 cut(s) 80, 81
BfaI CTAG 2 cut(s) 545, 675
BfuAI ACCTGC 1 cut(s) 166
BfuI GTATCC 1 cut(s) 595
BisI GCNGC 1 cut(s) 702
BlsI GCNGC 1 cut(s) 703
Bme1390I CCNGG 3 cut(s) 80, 81, 689
Bme18I GGWCC 2 cut(s) 600, 685
BmeT110I CYCGRG 1 cut(s) 79
BmgT120I GGNCC 4 cut(s) 76, 600, 623, 685
BmiI GGNNCC 2 cut(s) 78, 646
BmrFI CCNGG 3 cut(s) 80, 81, 689
BmrI ACTGGG 1 cut(s) 466
BmuI ACTGGG 1 cut(s) 466
BpmI CTGGAG 1 cut(s) 51
Bpu14I TTCGAA 1 cut(s) 208
BpuMI CCSGG 2 cut(s) 80, 81
BsaAI YACGTR 2 cut(s) 385, 401
BsaJI CCNNGG 4 cut(s) 72, 78, 79, 705
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 458
Bse1I ACTGG 1 cut(s) 472
BseBI CCWGG 1 cut(s) 689
BseDI CCNNGG 4 cut(s) 72, 78, 79, 705
BseLI CCNNNNNNNGG 2 cut(s) 136, 458
BseMII CTCAG 1 cut(s) 208
BseNI ACTGG 1 cut(s) 472
BseRI GAGGAG 1 cut(s) 403
BseYI CCCAGC 1 cut(s) 7
BsgI GTGCAG 1 cut(s) 194
BshFI GGCC 3 cut(s) 77, 625, 704
BsiHKAI GWGCWC 1 cut(s) 395
BsiHKCI CYCGRG 1 cut(s) 79
BsiSI CCGG 1 cut(s) 80
BslFI GGGAC 1 cut(s) 212
BslI CCNNNNNNNGG 2 cut(s) 136, 458
BsmFI GGGAC 1 cut(s) 212
BsnI GGCC 3 cut(s) 77, 625, 704
BsoBI CYCGRG 1 cut(s) 79
Bsp119I TTCGAA 1 cut(s) 208
Bsp1286I GDGCHC 1 cut(s) 395
Bsp143I GATC 2 cut(s) 166, 343
BspACI CCGC 2 cut(s) 396, 701
BspANI GGCC 3 cut(s) 77, 625, 704
BspCNI CTCAG 1 cut(s) 207
BspHI TCATGA 2 cut(s) 559, 657
BspLI GGNNCC 2 cut(s) 78, 646
BspMI ACCTGC 1 cut(s) 166
BspPI GGATC 1 cut(s) 174
BspT104I TTCGAA 1 cut(s) 208
BsrI ACTGG 1 cut(s) 472
BssECI CCNNGG 4 cut(s) 72, 78, 79, 705
BssMI GATC 2 cut(s) 166, 343
BssT1I CCWWGG 2 cut(s) 72, 705
Bst2UI CCWGG 1 cut(s) 689
Bst4CI ACNGT 4 cut(s) 203, 439, 557, 631
BstBAI YACGTR 2 cut(s) 385, 401
BstBI TTCGAA 1 cut(s) 208
BstDEI CTNAG 1 cut(s) 194
BstENI CCTNNNNNAGG 1 cut(s) 456
BstKTI GATC 2 cut(s) 169, 346
BstMBI GATC 2 cut(s) 166, 343
BstMWI GCNNNNNNNGC 1 cut(s) 539
BstNI CCWGG 1 cut(s) 689
BstSCI CCNGG 3 cut(s) 78, 79, 687
BsuI GTATCC 1 cut(s) 595
BsuRI GGCC 3 cut(s) 77, 625, 704
BtsIMutI CAGTG 1 cut(s) 636
BveI ACCTGC 1 cut(s) 166
CciI TCATGA 2 cut(s) 559, 657
Cfr13I GGNCC 4 cut(s) 76, 600, 623, 685
Cfr9I CCCGGG 1 cut(s) 79
CseI GACGC 1 cut(s) 143
Csp6I GTAC 2 cut(s) 382, 619
CspCI CAANNNNNGTGG 2 cut(s) 578, 613
CviAII CATG 2 cut(s) 560, 658
CviQI GTAC 2 cut(s) 382, 619
DdeI CTNAG 1 cut(s) 194
DpnI GATC 2 cut(s) 168, 345
DpnII GATC 2 cut(s) 166, 343
DraI TTTAAA 1 cut(s) 478
EaeI YGGCCR 1 cut(s) 702
Ecl136II GAGCTC 1 cut(s) 393
Eco130I CCWWGG 2 cut(s) 72, 705
Eco24I GRGCYC 1 cut(s) 395
Eco47I GGWCC 2 cut(s) 600, 685
Eco53kI GAGCTC 1 cut(s) 393
Eco72I CACGTG 1 cut(s) 401
Eco88I CYCGRG 1 cut(s) 79
EcoICRI GAGCTC 1 cut(s) 393
EcoNI CCTNNNNNAGG 1 cut(s) 456
EcoO109I RGGNCCY 1 cut(s) 76
EcoRI GAATTC 1 cut(s) 50
EcoRII CCWGG 1 cut(s) 687
EcoT14I CCWWGG 2 cut(s) 72, 705
EcoT38I GRGCYC 1 cut(s) 395
ErhI CCWWGG 2 cut(s) 72, 705
FaeI CATG 2 cut(s) 563, 661
FaiI YATR 7 cut(s) 18, 47, 414, 561, 582, 659, 721
FaqI GGGAC 1 cut(s) 212
FatI CATG 2 cut(s) 559, 657
Fnu4HI GCNGC 1 cut(s) 702
FriOI GRGCYC 1 cut(s) 395
Fsp4HI GCNGC 1 cut(s) 702
FspBI CTAG 2 cut(s) 545, 675
GluI GCNGC 1 cut(s) 702
GsaI CCCAGC 1 cut(s) 11
GsuI CTGGAG 1 cut(s) 51
HaeIII GGCC 3 cut(s) 77, 625, 704
HapII CCGG 1 cut(s) 80
HgaI GACGC 1 cut(s) 143
Hin1II CATG 2 cut(s) 563, 661
HinfI GANTC 6 cut(s) 12, 28, 41, 126, 216, 422
HpaII CCGG 1 cut(s) 80
Hpy166II GTNNAC 1 cut(s) 382
Hpy188I TCNGA 4 cut(s) 40, 110, 131, 457
Hpy188III TCNNGA 3 cut(s) 220, 560, 658
Hpy8I GTNNAC 1 cut(s) 382
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 4 cut(s) 114, 283, 452, 462
HpyCH4III ACNGT 4 cut(s) 203, 439, 557, 631
HpyCH4IV ACGT 2 cut(s) 384, 400
HpyCH4V TGCA 3 cut(s) 175, 359, 374
HpyF10VI GCNNNNNNNGC 1 cut(s) 539
HpyF3I CTNAG 1 cut(s) 194
HpySE526I ACGT 2 cut(s) 384, 400
Hsp92II CATG 2 cut(s) 563, 661
Kzo9I GATC 2 cut(s) 166, 343
LmnI GCTCC 4 cut(s) 4, 237, 390, 398
MaeI CTAG 2 cut(s) 545, 675
MaeII ACGT 2 cut(s) 384, 400
MaeIII GTNAC 2 cut(s) 256, 466
MalI GATC 2 cut(s) 168, 345
MboI GATC 2 cut(s) 166, 343
MboII GAAGA 2 cut(s) 96, 527
MhlI GDGCHC 1 cut(s) 395
MluCI AATT 6 cut(s) 21, 50, 90, 99, 308, 576
MlyI GAGTC 1 cut(s) 37
MmeI TCCRAC 1 cut(s) 504
MnlI CCTC 4 cut(s) 156, 288, 381, 520
MroXI GAANNNNTTC 1 cut(s) 408
MseI TTAA 6 cut(s) 273, 311, 441, 477, 489, 614
MslI CAYNNNNRTG 1 cut(s) 417
MspI CCGG 1 cut(s) 80
MspR9I CCNGG 3 cut(s) 80, 81, 689
MvaI CCWGG 1 cut(s) 689
MwoI GCNNNNNNNGC 1 cut(s) 539
NciI CCSGG 2 cut(s) 80, 81
NdeII GATC 2 cut(s) 166, 343
NlaIII CATG 2 cut(s) 563, 661
NlaIV GGNNCC 2 cut(s) 78, 646
NmuCI GTSAC 1 cut(s) 256
NspV TTCGAA 1 cut(s) 208
PagI TCATGA 2 cut(s) 559, 657
PaqCI CACCTGC 1 cut(s) 166
PdmI GAANNNNTTC 1 cut(s) 408
PfeI GAWTC 5 cut(s) 12, 41, 126, 216, 422
PkrI GCNGC 1 cut(s) 703
PleI GAGTC 1 cut(s) 36
PmaCI CACGTG 1 cut(s) 401
PmlI CACGTG 1 cut(s) 401
PpsI GAGTC 1 cut(s) 36
Ppu21I YACGTR 2 cut(s) 385, 401
Psp124BI GAGCTC 1 cut(s) 395
Psp6I CCWGG 1 cut(s) 687
PspCI CACGTG 1 cut(s) 401
PspFI CCCAGC 1 cut(s) 7
PspGI CCWGG 1 cut(s) 687
PspN4I GGNNCC 2 cut(s) 78, 646
PspPI GGNCC 4 cut(s) 76, 600, 623, 685
RsaI GTAC 2 cut(s) 383, 620
RsaNI GTAC 2 cut(s) 382, 619
RseI CAYNNNNRTG 1 cut(s) 417
SacI GAGCTC 1 cut(s) 395
SaqAI TTAA 6 cut(s) 273, 311, 441, 477, 489, 614
SatI GCNGC 1 cut(s) 702
Sau3AI GATC 2 cut(s) 166, 343
Sau96I GGNCC 4 cut(s) 76, 600, 623, 685
SchI GAGTC 1 cut(s) 37
ScrFI CCNGG 3 cut(s) 80, 81, 689
SduI GDGCHC 1 cut(s) 395
SetI ASST 9 cut(s) 9, 180, 324, 387, 395, 403, 544, 620, 693
SfuI TTCGAA 1 cut(s) 208
SinI GGWCC 2 cut(s) 600, 685
SmaI CCCGGG 1 cut(s) 81
SmiMI CAYNNNNRTG 1 cut(s) 417
Sse9I AATT 6 cut(s) 21, 50, 90, 99, 308, 576
SsiI CCGC 2 cut(s) 396, 701
SspI AATATT 2 cut(s) 482, 713
SspMI CTAG 2 cut(s) 545, 675
SstI GAGCTC 1 cut(s) 395
StyD4I CCNGG 3 cut(s) 78, 79, 687
StyI CCWWGG 2 cut(s) 72, 705
TaaI ACNGT 4 cut(s) 203, 439, 557, 631
TaiI ACGT 2 cut(s) 387, 403
TaqI TCGA 2 cut(s) 208, 342
TasI AATT 6 cut(s) 21, 50, 90, 99, 308, 576
TauI GCSGC 1 cut(s) 704
TfiI GAWTC 5 cut(s) 12, 41, 126, 216, 422
Tru1I TTAA 6 cut(s) 273, 311, 441, 477, 489, 614
Tru9I TTAA 6 cut(s) 273, 311, 441, 477, 489, 614
TscAI CASTG 1 cut(s) 636
TseFI GTSAC 1 cut(s) 256
Tsp45I GTSAC 1 cut(s) 256
TspDTI ATGAA 2 cut(s) 401, 646
TspGWI ACGGA 1 cut(s) 419
TspMI CCCGGG 1 cut(s) 79
TspRI CASTG 1 cut(s) 636
VpaK11BI GGWCC 2 cut(s) 600, 685
XagI CCTNNNNNAGG 1 cut(s) 456
XapI RAATTY 2 cut(s) 21, 50
XmaI CCCGGG 1 cut(s) 79
XmnI GAANNNNTTC 1 cut(s) 408
XspI CTAG 2 cut(s) 545, 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.