Rroxscaffold_6G00414550

ATP-dependent zinc metalloprotease FTSH 10, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002584
Physical Location & Seq
Reverse (-)
36866253 .. 36871240
4988 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00414550.1

Sequence Viewer

Length: 2433 bp
ATGATTTTCTCCAGCGGCGGACGGCTCCTCTCTCGCTCGGCTCGTTCCGCTTTCCAAAAGAACGTAATTTGGGTTAATTATAATGGGAGAGCTGCGCTTAGGCACGAACCGCCGACGCCGCTTGGCGGCAATGCGGGCATTTCCGGCGGGTTAGGGCTTCTGAGGGGGTATTTAACTTATAGTGGAGCTGGGAAGCAGCTCGTTTCGAATACCTATATGTCTAATTTGAAATCGGCTCTTGCAAACCCTAGGATTCGTCGCCTGTTCTCCAGCGAAAGGCCTGGGAAGAAAAGTAAGACGTTTTCCTGCTCAAAAGTTAGAGGAAAAGATTACGAGAATTATTATCCGAAAGATAAGAAGGAAATTCGAAAGGGAATGATCAGAAATCCGGAAGGTGCTGGTGATCAGGGGAATACCCAGGGAAATTTTTCAAAGCAGATTCAAGATTTAATCACTCCTTTATTGTTCATGGGATTTATTTTCATATCGTTATTCCTTACTCCTCAGAAACAGAAGCAGGCAATCACTACTCTTAGAGCAATTGCTTTACTAGTGTTGAATAGGTGGATAGTTCTCCTTCCGGAGGAAAATTTGAGCCAGTACAAGTACTATTTTAACATTGGGAGTGTTGACTCTTTTGAGGAAAAATTGGAGGAAGCCCAAGAAGCTTTAGGGATTGACCGTCATGATTTTGTTCCTGTAATTTATGTAGCGCAAATCAATTGGTTCCAAGAGCTGATGAAGTATGCGCCAACAATGTTGCTTTTGGGAGCTCTTTGGTTCATGGGGCGAAAAATGCCTGGTATTGGTGGCACAGGTGGAAAAGGTGGGAAAGGAATATTCAATATAGGAAAAGCACAGATAACAAAAGTGGATAAGAATGCCAAAAACAAGGTTTTCTTTAAAGATGTAGCTGGCTGTGATGAGGCTAAGCAAGAAATTATGGAATTTGTGCACTTTCTCAAGAACCCTAAGAAATATGAGGAATTGGGAGCAAAAATTCCAAAGGGTGCCCTTCTTGTTGGCCCTCCCGGAACTGGAAAAACTCTTATTGCAAAGGCAACTGCAGGTGAATCTGGGGTGCCATTTCTATCTATATCTGGTTCGGATTTTATGGAAATGTTTGTTGGTGTTGGGCCATCAAGGGTTCGGAGTTTATTTCAAGAGGCAAGACAATGTGCACCTAGTATAATATTTATTGATGAGATTGATGCAATTGGCCGGGCAAGAGGGCGTGGAGGTTTTTCAGGTGGCAATGATGAGCGAGAAAGTACTCTCAATCAATTGCTTGTAGAAATGGATGGATTTGGAACAACTGCTGGAGTTGTTGTACTTGCTGGGACCAATAGGCCTGATATTCTGGATAAAGCACTCTTGAGGCCTGGTCGGTTTGACCGTCAAATTACTATTGATAAACCAGACATTAAAGGTCGTAACCAGATTTTTCAGATATATCTGAAAAAGCTCAAATTAGATCGCAAACCATCCCATTACTCAGAGAGACTTGCTGCTCTGACTCCTGGATTCGCTGGAGCAGATATAGCAAATGTCTGCAATGAAGCTGCCTTGATTGCTGCAAGGAATGAGAGTGCAAAGATCACAATGAAACATTTTGAGGCAGCTATCGATCGGGTAATTGGCGGTCTTGAAAAGAAGAACAAGGTAATAAGCAAGCTGGAAAGACGGACTGTTGCTTACCATGAAGCTGGCCATGCTGTTGCTGGTTGGTTCTTAGAACATGCAGAACCTTTACTTAAAGTAACTATTGTTCCACGTGGCACTGCAGCACTGGGGTTTGCTCAGTATGTCCCCAATGAAAATCTTCTTATGACCAAAGAGCAGCTTTTTGATATGACATGCATGACCCTTGGTGGTCGAGCTTCTGAAGAGGTGAAGTTACTGTCTCTACTTATCCAGAACATTTTTAATCAGCAAGTAGACAAATTAAAGGTTTTGTTGGGGAAGATTTCAACTGGAGCACAGAACGACTTGGAGAAAGTGACCAAAATGACATATGCCCAAGTTGCAGTATATGGCTTCAGCGACAAAGTGGGCCTTCTTTCTTTTCCTCAAAGGGATGAATTTGAGATGACGAAGCCTTACAGTAGTAAGACTGGTGCAATAATTGACAGTGAAGTTAGAGAATGGGTTGCCAAAGCATATACCCGTACTATTGAGCTGATAGAGGAGCACAAGGAGCACGTAGGACAGATTGCAGAGCTGTTGCTGGAAAAGGAGGTCCTACACCAAGAAGACTTGATTCGAGTCTTGGGAGAGCGCCCATGGAAGTCGGAGGAGCCCACAAATTATGATAGATTCAAGCAAGGGTTCCAAGAAGAAGAAGATAAGGAGGCTAAAGAGACAACCACTGAGGGTAAGGATGTGAAGGATGGTCGGTCACCGCCTCTTCAACCAGATGCTGTGCCTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA FtsH_ext Peptidase_M41

Protein Analysis

810

Amino Acids

89.87

Weight (kDa)

9.22

Isoelectric Point (pI)

33.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 339 - 471 2.2e-42 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 500 - 540 3.6e-13 AAA+ lid domain
Peptidase_M41 PF01434 555 - 632 1.8e-28 Peptidase family M41
Peptidase_M41 PF01434 651 - 755 4.2e-28 Peptidase family M41
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 81
AarI CACCTGC 1 cut(s) 1058
Acc36I ACCTGC 1 cut(s) 1058
AccB1I GGYRCC 2 cut(s) 1010, 1081
AccI GTMKAC 1 cut(s) 1938
AccIII TCCGGA 2 cut(s) 388, 580
AcoI YGGCCR 2 cut(s) 1219, 1708
AcsI RAATTY 6 cut(s) 363, 424, 589, 947, 999, 2081
AcuI CTGAAG 2 cut(s) 1905, 2023
AcvI CACGTG 1 cut(s) 1775
AcyI GRCGYC 1 cut(s) 116
AfaI GTAC 5 cut(s) 602, 608, 1273, 1332, 2170
AfiI CCNNNNNNNGG 5 cut(s) 125, 276, 583, 806, 1037
AhlI ACTAGT 1 cut(s) 550
AjnI CCWGG 5 cut(s) 280, 417, 799, 1381, 1519
AjuI GAANNNNNNNTTGG 2 cut(s) 1110, 1142
Alw21I GWGCWC 6 cut(s) 775, 957, 1183, 1981, 2193, 2202
Alw26I GTCTC 3 cut(s) 1495, 1908, 2354
Alw44I GTGCAC 2 cut(s) 953, 1179
AlwNI CAGNNNCTG 1 cut(s) 2420
Aor13HI TCCGGA 2 cut(s) 388, 580
AoxI GGCC 8 cut(s) 278, 1024, 1136, 1219, 1349, 1379, 1708, 2053
ApaLI GTGCAC 2 cut(s) 953, 1179
ApeKI GCWGC 8 cut(s) 92, 196, 1508, 1562, 1574, 1619, 1784, 1840
ApoI RAATTY 6 cut(s) 363, 424, 589, 947, 999, 2081
Asp700I GAANNNNTTC 2 cut(s) 427, 1821
AspA2I CCTAGG 1 cut(s) 248
AspLEI GCGC 4 cut(s) 97, 715, 751, 2280
AspS9I GGNCC 5 cut(s) 1025, 1136, 1341, 2053, 2239
AsuC2I CCSGG 2 cut(s) 1032, 1223
AsuHPI GGTGA 4 cut(s) 413, 1082, 1903, 2391
AsuII TTCGAA 2 cut(s) 206, 367
AvaII GGWCC 2 cut(s) 1341, 2239
AvrII CCTAGG 1 cut(s) 248
BaeGI GKGCMC 3 cut(s) 957, 1015, 1183
BalI TGGCCA 1 cut(s) 1710
BanI GGYRCC 2 cut(s) 1010, 1081
BanII GRGCYC 2 cut(s) 775, 2301
BbrPI CACGTG 1 cut(s) 1775
BbsI GAAGAC 1 cut(s) 2259
Bbv12I GWGCWC 6 cut(s) 775, 957, 1183, 1981, 2193, 2202
BbvI GCAGC 8 cut(s) 79, 208, 1495, 1549, 1561, 1631, 1796, 1852
BccI CCATC 4 cut(s) 1147, 1295, 1492, 2384
BceAI ACGGC 1 cut(s) 38
BciT130I CCWGG 5 cut(s) 282, 419, 801, 1383, 1521
BclI TGATCA 2 cut(s) 378, 403
BcnI CCSGG 2 cut(s) 1032, 1223
BcoDI GTCTC 3 cut(s) 1495, 1908, 2354
BcuI ACTAGT 1 cut(s) 550
BfaI CTAG 3 cut(s) 249, 551, 1185
BfmI CTRYAG 2 cut(s) 1065, 1782
BfoI RGCGCY 1 cut(s) 2281
BfuAI ACCTGC 1 cut(s) 1058
BlnI CCTAGG 1 cut(s) 248
BlpI GCTNAGC 1 cut(s) 930
BmcAI AGTACT 2 cut(s) 608, 1273
Bme1390I CCNGG 7 cut(s) 282, 419, 801, 1032, 1223, 1383, 1521
Bme18I GGWCC 2 cut(s) 1341, 2239
BmgT120I GGNCC 5 cut(s) 1025, 1136, 1341, 2053, 2239
BmiI GGNNCC 7 cut(s) 26, 728, 1012, 1083, 1342, 2298, 2330
BmrFI CCNGG 7 cut(s) 282, 419, 801, 1032, 1223, 1383, 1521
BmrI ACTGGG 1 cut(s) 1799
BmsI GCATC 2 cut(s) 1201, 2407
BmuI ACTGGG 1 cut(s) 1799
BpiI GAAGAC 1 cut(s) 2259
BpmI CTGGAG 4 cut(s) 253, 1341, 1551, 1995
Bpu10I CCTNAGC 1 cut(s) 98
Bpu1102I GCTNAGC 1 cut(s) 930
Bpu14I TTCGAA 2 cut(s) 206, 367
BpuEI CTTGAG 2 cut(s) 947, 1396
BpuMI CCSGG 2 cut(s) 1032, 1223
Bsa29I ATCGAT 1 cut(s) 1626
BsaAI YACGTR 2 cut(s) 1775, 2203
BsaHI GRCGYC 1 cut(s) 116
BsaJI CCNNGG 6 cut(s) 248, 281, 417, 418, 1867, 2282
BsaWI WCCGGW 2 cut(s) 388, 580
BsaXI ACNNNNNCTCC 2 cut(s) 1314, 1344
Bsc4I CCNNNNNNNGG 5 cut(s) 125, 276, 583, 806, 1037
Bse1I ACTGG 5 cut(s) 598, 1042, 1794, 1978, 2119
Bse3DI GCAATG 3 cut(s) 136, 1261, 1561
BseAI TCCGGA 2 cut(s) 388, 580
BseBI CCWGG 5 cut(s) 282, 419, 801, 1383, 1521
BseCI ATCGAT 1 cut(s) 1626
BseDI CCNNGG 6 cut(s) 248, 281, 417, 418, 1867, 2282
BseGI GGATG 5 cut(s) 1306, 1484, 2083, 2386, 2395
BseLI CCNNNNNNNGG 5 cut(s) 125, 276, 583, 806, 1037
BseMI GCAATG 3 cut(s) 136, 1261, 1561
BseMII CTCAG 5 cut(s) 152, 518, 1509, 1814, 2361
BseNI ACTGG 5 cut(s) 598, 1042, 1794, 1978, 2119
BseRI GAGGAG 4 cut(s) 17, 492, 2201, 2309
BseSI GKGCMC 3 cut(s) 957, 1015, 1183
BseXI GCAGC 8 cut(s) 79, 208, 1495, 1549, 1561, 1631, 1796, 1852
BseYI CCCAGC 2 cut(s) 188, 1337
Bsh1285I CGRYCG 1 cut(s) 1630
BshFI GGCC 8 cut(s) 280, 1026, 1138, 1221, 1351, 1381, 1710, 2055
BshNI GGYRCC 2 cut(s) 1010, 1081
BshVI ATCGAT 1 cut(s) 1626
BsiEI CGRYCG 1 cut(s) 1630
BsiHKAI GWGCWC 6 cut(s) 775, 957, 1183, 1981, 2193, 2202
BsiSI CCGG 5 cut(s) 144, 389, 581, 1032, 1222
BslFI GGGAC 2 cut(s) 1354, 1793
BslI CCNNNNNNNGG 5 cut(s) 125, 276, 583, 806, 1037
BsmAI GTCTC 3 cut(s) 1495, 1908, 2354
BsmFI GGGAC 2 cut(s) 1354, 1793
BsmI GAATGC 1 cut(s) 886
BsnI GGCC 8 cut(s) 280, 1026, 1138, 1221, 1351, 1381, 1710, 2055
Bsp119I TTCGAA 2 cut(s) 206, 367
Bsp1286I GDGCHC 8 cut(s) 775, 957, 1015, 1183, 1981, 2193, 2202, 2301
Bsp13I TCCGGA 2 cut(s) 388, 580
Bsp143I GATC 5 cut(s) 378, 403, 1474, 1596, 1627
Bsp1720I GCTNAGC 1 cut(s) 930
Bsp19I CCATGG 1 cut(s) 2282
BspANI GGCC 8 cut(s) 280, 1026, 1138, 1221, 1351, 1381, 1710, 2055
BspCNI CTCAG 5 cut(s) 153, 517, 1508, 1813, 2362
BspDI ATCGAT 1 cut(s) 1626
BspEI TCCGGA 2 cut(s) 388, 580
BspHI TCATGA 1 cut(s) 685
BspLI GGNNCC 7 cut(s) 26, 728, 1012, 1083, 1342, 2298, 2330
BspMAI CTGCAG 2 cut(s) 1069, 1786
BspMI ACCTGC 1 cut(s) 1058
BspT104I TTCGAA 2 cut(s) 206, 367
BspT107I GGYRCC 2 cut(s) 1010, 1081
BsrDI GCAATG 3 cut(s) 136, 1261, 1561
BsrI ACTGG 5 cut(s) 598, 1042, 1794, 1978, 2119
BssECI CCNNGG 6 cut(s) 248, 281, 417, 418, 1867, 2282
BssMI GATC 5 cut(s) 378, 403, 1474, 1596, 1627
BssNI GRCGYC 1 cut(s) 116
BssT1I CCWWGG 3 cut(s) 248, 1867, 2282
Bst2UI CCWGG 5 cut(s) 282, 419, 801, 1383, 1521
Bst4CI ACNGT 6 cut(s) 683, 1397, 1690, 1902, 2105, 2132
Bst6I CTCTTC 2 cut(s) 1881, 2412
BstACI GRCGYC 1 cut(s) 116
BstBAI YACGTR 2 cut(s) 1775, 2203
BstBI TTCGAA 2 cut(s) 206, 367
BstC8I GCNNGC 6 cut(s) 136, 519, 916, 1673, 1708, 2427
BstDSI CCRYGG 1 cut(s) 2282
BstEII GGTNACC 1 cut(s) 2397
BstENI CCTNNNNNAGG 1 cut(s) 581
BstF5I GGATG 5 cut(s) 1306, 1484, 2083, 2386, 2395
BstH2I RGCGCY 1 cut(s) 2281
BstHHI GCGC 4 cut(s) 97, 715, 751, 2280
BstKTI GATC 5 cut(s) 381, 406, 1477, 1599, 1630
BstMAI GTCTC 3 cut(s) 1495, 1908, 2354
BstMBI GATC 5 cut(s) 378, 403, 1474, 1596, 1627
BstMCI CGRYCG 1 cut(s) 1630
BstNI CCWGG 5 cut(s) 282, 419, 801, 1383, 1521
BstNSI RCATGY 2 cut(s) 1742, 1860
BstPI GGTNACC 1 cut(s) 2397
BstSCI CCNGG 7 cut(s) 280, 417, 799, 1030, 1221, 1381, 1519
BstSFI CTRYAG 2 cut(s) 1065, 1782
BstSLI GKGCMC 3 cut(s) 957, 1015, 1183
BstV1I GCAGC 8 cut(s) 79, 208, 1495, 1549, 1561, 1631, 1796, 1852
BstV2I GAAGAC 1 cut(s) 2259
BstXI CCANNNNNNTGG 1 cut(s) 1706
Bsu15I ATCGAT 1 cut(s) 1626
BsuRI GGCC 8 cut(s) 280, 1026, 1138, 1221, 1351, 1381, 1710, 2055
BsuTUI ATCGAT 1 cut(s) 1626
BtgI CCRYGG 1 cut(s) 2282
BtsCI GGATG 5 cut(s) 1306, 1484, 2083, 2386, 2395
BtsI GCAGTG 1 cut(s) 1779
BtsIMutI CAGTG 4 cut(s) 1779, 1787, 2137, 2367
BveI ACCTGC 1 cut(s) 1058
Cac8I GCNNGC 6 cut(s) 136, 519, 916, 1673, 1708, 2427
CaiI CAGNNNCTG 1 cut(s) 2420
CciI TCATGA 1 cut(s) 685
CfoI GCGC 4 cut(s) 97, 715, 751, 2280
Cfr13I GGNCC 5 cut(s) 1025, 1136, 1341, 2053, 2239
ClaI ATCGAT 1 cut(s) 1626
CseI GACGC 1 cut(s) 124
Csp6I GTAC 5 cut(s) 601, 607, 1272, 1331, 2169
CviAII CATG 9 cut(s) 469, 686, 784, 1700, 1712, 1739, 1857, 1861, 2283
CviQI GTAC 5 cut(s) 601, 607, 1272, 1331, 2169
DpnI GATC 5 cut(s) 380, 405, 1476, 1598, 1629
DpnII GATC 5 cut(s) 378, 403, 1474, 1596, 1627
DraI TTTAAA 1 cut(s) 904
EaeI YGGCCR 2 cut(s) 1219, 1708
Eam1104I CTCTTC 2 cut(s) 1881, 2412
EarI CTCTTC 2 cut(s) 1881, 2412
EciI GGCGGA 1 cut(s) 33
Ecl136II GAGCTC 1 cut(s) 773
Eco130I CCWWGG 3 cut(s) 248, 1867, 2282
Eco147I AGGCCT 3 cut(s) 280, 1351, 1381
Eco24I GRGCYC 2 cut(s) 775, 2301
Eco47I GGWCC 2 cut(s) 1341, 2239
Eco53kI GAGCTC 1 cut(s) 773
Eco57I CTGAAG 2 cut(s) 1905, 2023
Eco72I CACGTG 1 cut(s) 1775
Eco91I GGTNACC 1 cut(s) 2397
EcoICRI GAGCTC 1 cut(s) 773
EcoNI CCTNNNNNAGG 1 cut(s) 581
EcoO109I RGGNCCY 1 cut(s) 2239
EcoO65I GGTNACC 1 cut(s) 2397
EcoRII CCWGG 5 cut(s) 280, 417, 799, 1381, 1519
EcoT14I CCWWGG 3 cut(s) 248, 1867, 2282
EcoT22I ATGCAT 1 cut(s) 1862
EcoT38I GRGCYC 2 cut(s) 775, 2301
ErhI CCWWGG 3 cut(s) 248, 1867, 2282
FaeI CATG 9 cut(s) 472, 689, 787, 1703, 1715, 1742, 1860, 1864, 2286
FalI AAGNNNNNCTT 6 cut(s) 884, 916, 1827, 1859, 2040, 2072
FaqI GGGAC 2 cut(s) 1354, 1793
FatI CATG 9 cut(s) 468, 685, 783, 1699, 1711, 1738, 1856, 1860, 2282
FauI CCCGC 2 cut(s) 127, 140
FauNDI CATATG 1 cut(s) 2014
FbaI TGATCA 2 cut(s) 378, 403
FblI GTMKAC 1 cut(s) 1938
FokI GGATG 5 cut(s) 1313, 1471, 2090, 2393, 2402
FriOI GRGCYC 2 cut(s) 775, 2301
FspBI CTAG 3 cut(s) 249, 551, 1185
GlaI GCGC 4 cut(s) 96, 714, 750, 2279
GsaI CCCAGC 2 cut(s) 192, 1341
GsuI CTGGAG 4 cut(s) 253, 1341, 1551, 1995
HaeII RGCGCY 1 cut(s) 2281
HaeIII GGCC 8 cut(s) 280, 1026, 1138, 1221, 1351, 1381, 1710, 2055
HapII CCGG 5 cut(s) 144, 389, 581, 1032, 1222
HgaI GACGC 1 cut(s) 124
HhaI GCGC 4 cut(s) 97, 715, 751, 2280
Hin1I GRCGYC 1 cut(s) 116
Hin1II CATG 9 cut(s) 472, 689, 787, 1703, 1715, 1742, 1860, 1864, 2286
Hin6I GCGC 4 cut(s) 95, 713, 749, 2278
HinP1I GCGC 4 cut(s) 95, 713, 749, 2278
HincII GTYRAC 1 cut(s) 631
HindII GTYRAC 1 cut(s) 631
HindIII AAGCTT 1 cut(s) 666
HinfI GANTC 9 cut(s) 253, 439, 632, 1073, 1516, 1524, 2260, 2265, 2316
HpaII CCGG 5 cut(s) 144, 389, 581, 1032, 1222
HphI GGTGA 4 cut(s) 413, 1082, 1903, 2391
Hpy166II GTNNAC 4 cut(s) 631, 955, 1181, 1939
Hpy8I GTNNAC 4 cut(s) 631, 955, 1181, 1939
Hpy99I CGWCG 2 cut(s) 118, 261
HpyAV CCTTC 6 cut(s) 352, 386, 587, 1025, 2066, 2380
HpyCH4III ACNGT 6 cut(s) 683, 1397, 1690, 1902, 2105, 2132
HpyCH4IV ACGT 4 cut(s) 63, 299, 1774, 2202
HpySE526I ACGT 4 cut(s) 63, 299, 1774, 2202
Hsp92I GRCGYC 1 cut(s) 116
Hsp92II CATG 9 cut(s) 472, 689, 787, 1703, 1715, 1742, 1860, 1864, 2286
HspAI GCGC 4 cut(s) 95, 713, 749, 2278
Kpn2I TCCGGA 2 cut(s) 388, 580
Ksp22I TGATCA 2 cut(s) 378, 403
Kzo9I GATC 5 cut(s) 378, 403, 1474, 1596, 1627
LmnI GCTCC 9 cut(s) 30, 185, 770, 992, 1532, 1976, 2188, 2197, 2296
Lsp1109I GCAGC 8 cut(s) 79, 208, 1495, 1549, 1561, 1631, 1796, 1852
LweI GCATC 2 cut(s) 1201, 2407
MaeI CTAG 3 cut(s) 249, 551, 1185
MaeII ACGT 4 cut(s) 63, 299, 1774, 2202
MaeIII GTNAC 5 cut(s) 1433, 1759, 1896, 1999, 2397
MalI GATC 5 cut(s) 380, 405, 1476, 1598, 1629
MboI GATC 5 cut(s) 378, 403, 1474, 1596, 1627
MfeI CAATTG 4 cut(s) 540, 721, 1215, 1283
MhlI GDGCHC 8 cut(s) 775, 957, 1015, 1183, 1981, 2193, 2202, 2301
MlsI TGGCCA 1 cut(s) 1710
MluNI TGGCCA 1 cut(s) 1710
MlyI GAGTC 3 cut(s) 626, 1510, 2274
MmeI TCCRAC 1 cut(s) 2271
Mox20I TGGCCA 1 cut(s) 1710
Mph1103I ATGCAT 1 cut(s) 1862
MroI TCCGGA 2 cut(s) 388, 580
MroXI GAANNNNTTC 2 cut(s) 427, 1821
MscI TGGCCA 1 cut(s) 1710
MseI TTAA 9 cut(s) 75, 173, 449, 615, 903, 1425, 1755, 1926, 1946
Msp20I TGGCCA 1 cut(s) 1710
MspA1I CMGCKG 1 cut(s) 15
MspI CCGG 5 cut(s) 144, 389, 581, 1032, 1222
MspR9I CCNGG 7 cut(s) 282, 419, 801, 1032, 1223, 1383, 1521
MunI CAATTG 4 cut(s) 540, 721, 1215, 1283
Mva1269I GAATGC 1 cut(s) 886
MvaI CCWGG 5 cut(s) 282, 419, 801, 1383, 1521
NciI CCSGG 2 cut(s) 1032, 1223
NcoI CCATGG 1 cut(s) 2282
NdeI CATATG 1 cut(s) 2014
NdeII GATC 5 cut(s) 378, 403, 1474, 1596, 1627
NlaIII CATG 9 cut(s) 472, 689, 787, 1703, 1715, 1742, 1860, 1864, 2286
NlaIV GGNNCC 7 cut(s) 26, 728, 1012, 1083, 1342, 2298, 2330
NmeAIII GCCGAG 1 cut(s) 17
NmuCI GTSAC 2 cut(s) 1999, 2397
NsiI ATGCAT 1 cut(s) 1862
NspI RCATGY 2 cut(s) 1742, 1860
NspV TTCGAA 2 cut(s) 206, 367
PagI TCATGA 1 cut(s) 685
PaqCI CACCTGC 1 cut(s) 1058
PasI CCCWGGG 1 cut(s) 418
PceI AGGCCT 3 cut(s) 280, 1351, 1381
PcsI WCGNNNNNNNCGW 2 cut(s) 40, 1393
PctI GAATGC 1 cut(s) 886
PdmI GAANNNNTTC 2 cut(s) 427, 1821
PfeI GAWTC 6 cut(s) 253, 439, 1073, 1524, 2260, 2316
PfoI TCCNGGA 2 cut(s) 1030, 1519
Ple19I CGATCG 1 cut(s) 1630
PleI GAGTC 3 cut(s) 626, 1510, 2273
PmaCI CACGTG 1 cut(s) 1775
PmlI CACGTG 1 cut(s) 1775
PpsI GAGTC 3 cut(s) 626, 1510, 2273
Ppu21I YACGTR 2 cut(s) 1775, 2203
PpuMI RGGWCCY 1 cut(s) 2239
PsiI TTATAA 1 cut(s) 81
Psp124BI GAGCTC 1 cut(s) 775
Psp5II RGGWCCY 1 cut(s) 2239
Psp6I CCWGG 5 cut(s) 280, 417, 799, 1381, 1519
PspCI CACGTG 1 cut(s) 1775
PspEI GGTNACC 1 cut(s) 2397
PspFI CCCAGC 2 cut(s) 188, 1337
PspGI CCWGG 5 cut(s) 280, 417, 799, 1381, 1519
PspN4I GGNNCC 7 cut(s) 26, 728, 1012, 1083, 1342, 2298, 2330
PspPI GGNCC 5 cut(s) 1025, 1136, 1341, 2053, 2239
PspPPI RGGWCCY 1 cut(s) 2239
PsrI GAACNNNNNNTAC 2 cut(s) 1752, 1784
PstI CTGCAG 2 cut(s) 1069, 1786
PstNI CAGNNNCTG 1 cut(s) 2420
PvuI CGATCG 1 cut(s) 1630
RsaI GTAC 5 cut(s) 602, 608, 1273, 1332, 2170
RsaNI GTAC 5 cut(s) 601, 607, 1272, 1331, 2169
SacI GAGCTC 1 cut(s) 775
SaqAI TTAA 9 cut(s) 75, 173, 449, 615, 903, 1425, 1755, 1926, 1946
Sau3AI GATC 5 cut(s) 378, 403, 1474, 1596, 1627
Sau96I GGNCC 5 cut(s) 1025, 1136, 1341, 2053, 2239
ScaI AGTACT 2 cut(s) 608, 1273
SchI GAGTC 3 cut(s) 626, 1510, 2274
ScrFI CCNGG 7 cut(s) 282, 419, 801, 1032, 1223, 1383, 1521
SduI GDGCHC 8 cut(s) 775, 957, 1015, 1183, 1981, 2193, 2202, 2301
SfaNI GCATC 2 cut(s) 1201, 2407
SfcI CTRYAG 2 cut(s) 1065, 1782
SfuI TTCGAA 2 cut(s) 206, 367
SinI GGWCC 2 cut(s) 1341, 2239
SmlI CTYRAG 2 cut(s) 962, 1375
SmoI CTYRAG 2 cut(s) 962, 1375
SpeI ACTAGT 1 cut(s) 550
SseBI AGGCCT 3 cut(s) 280, 1351, 1381
SspI AATATT 2 cut(s) 840, 1194
SspMI CTAG 3 cut(s) 249, 551, 1185
SstI GAGCTC 1 cut(s) 775
StuI AGGCCT 3 cut(s) 280, 1351, 1381
StyD4I CCNGG 7 cut(s) 280, 417, 799, 1030, 1221, 1381, 1519
StyI CCWWGG 3 cut(s) 248, 1867, 2282
TaaI ACNGT 6 cut(s) 683, 1397, 1690, 1902, 2105, 2132
TaiI ACGT 4 cut(s) 66, 302, 1777, 2205
TaqI TCGA 5 cut(s) 206, 367, 1626, 1876, 2263
TaqII GACCGA 1 cut(s) 2385
TatI WGTACW 4 cut(s) 600, 606, 1271, 1330
TauI GCSGC 3 cut(s) 18, 121, 129
TfiI GAWTC 6 cut(s) 253, 439, 1073, 1524, 2260, 2316
Tru1I TTAA 9 cut(s) 75, 173, 449, 615, 903, 1425, 1755, 1926, 1946
Tru9I TTAA 9 cut(s) 75, 173, 449, 615, 903, 1425, 1755, 1926, 1946
TscAI CASTG 4 cut(s) 1786, 1794, 2137, 2374
TseFI GTSAC 2 cut(s) 1999, 2397
TseI GCWGC 8 cut(s) 92, 196, 1508, 1562, 1574, 1619, 1784, 1840
Tsp45I GTSAC 2 cut(s) 1999, 2397
TspDTI ATGAA 9 cut(s) 457, 472, 755, 772, 1572, 1619, 1716, 1830, 2094
TspGWI ACGGA 1 cut(s) 1699
TspRI CASTG 4 cut(s) 1786, 1794, 2137, 2374
VneI GTGCAC 2 cut(s) 953, 1179
VpaK11BI GGWCC 2 cut(s) 1341, 2239
XagI CCTNNNNNAGG 1 cut(s) 581
XapI RAATTY 6 cut(s) 363, 424, 589, 947, 999, 2081
XceI RCATGY 2 cut(s) 1742, 1860
XcmI CCANNNNNNNNNTGG 1 cut(s) 1718
XmaJI CCTAGG 1 cut(s) 248
XmiI GTMKAC 1 cut(s) 1938
XmnI GAANNNNTTC 2 cut(s) 427, 1821
XspI CTAG 3 cut(s) 249, 551, 1185
ZrmI AGTACT 2 cut(s) 608, 1273
Zsp2I ATGCAT 1 cut(s) 1862
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.