MD04G1130900.v1.1

ATP-dependent zinc metalloprotease FTSH 10, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002584
Physical Location & Seq
Reverse (-)
21788325 .. 21793201
4877 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1130900.v1.1.491

Sequence Viewer

Length: 2448 bp
ATGATTTTCTCAAGCATCGGACGCTGGATCACCCACTCGGCTCGTTCCAAATTCAAAAGAAATGTGATTTCGGGTACTTACAACAGGAGGAACACGCTGCTGCACGATTCCTTTCCGCAGTTGTCGACGCTGCTCGGCAATGAGTGCGTTGATGGCGGCGTAGGGATTCTGAAGGGATATATAGCTTATAATGGAGCTGGGAAGCGGCTAATTTCAAAAGCATACATGTCGAATGTGAAGTCTGTTTTCGGAATCCCTAGAATTCGTCGCTTGTTCTCCAGCCAAGGTCCCGAGAAGAAAAATTACGAAAATTATTATCCGAAAAATAAGAAGGAAATTCCGAAAGGGGGTGAACAGAAAACGGAGTCCAAAGAGGGCTCAAGTGCAGGTGGTCAGGGGAATGCCCAGGGACAGTTTTCGAAGCAGATTCAAGATTTACTTGCTCCTTTAATGTTCATTTGCTTTATTGTCACATCATTATTCCTTAATCCTCATCAAGCGAAGGAGATAAGTTTCCAAGAATTTAAAAACAAGCTACTTGAACCTGGGTTGGTCGATCACATTGAAGTTGCAAACAAATCAGTTGCAAAAGTGTATGTGAGGAGCTCCCCACGCGAAAAAGTTCATAGTGATGATTCTGTTAGTAGTCCTGTTAAAGGTTCTCCTTCCGAAGGAAATGTAACCCAGTATAAATATTATTTTAACATTGGGAGTGTTGAATCTTTCGAAGAAAAGTTGGAGGAAGCTCAAGAAGCTTTAGGGATTGATCGTCATGATTTTGTTCCTGTAATTTATGCATCCCAAGTAATGTGGTTGCAAGAGTTGTTAAGGTATGGGCCAACAGTGTTGCTTCTGGGAGCCCTTTGGTTCATGAGCCGAAAAATGCCTAACATTGGTGGTCCAGGTGGAACAGGTGGCCGAGGCATATTCAACATAGGTAAAGCACAGATCACGAAGTTGGACAAGAATGCCAAAAACAAGGTTTACTTTAAAGATGTAGCGGGCTGTGATGAGGCTAAGCAAGAAATAATGGAGTTTGTGCACTTTCTAAAAAATCCTAAGAAATATGAGGAATTGGGAGCAAAAATTCCAAAAGGTGCTCTTCTTGTTGGCCCTCCTGGAACAGGAAAGACGCTTCTTGCAAAGGCAACTGCTGGTGAATCTGGGGTGCCTTTTCTATCTATATCTGGTTCAGATTTTATGGAAATGTTTGTTGGTGTTGGTCCGTCAAGGGTTCGAAGCTTATTTCAAGAGGCAAGACAGTGCGCACCCAGTATAATATTTATTGATGAGATTGATGCAATTGGCCGGGCAAGAGGGCGTGGAGGCTTTTCAGGTGGCCATGACGAGCGTGAAAGTACTCTCAATCAATTGCTTGTAGAAATGGATGGATTTGGAACCACTGCTGGAGTTGTTGTACTTGCTGGCACTAATAGGCCTGATATTCTGGATAAAGCTCTCTTGAGGCCAGGCCGGTTTGACCGTCAAATTACTATTGATAAACCAGACATCAAAGGTCGTAACCAGATTTTTCAAATATATCTGAGCAAGCTCAAGCTGGATCTGGAAGCATCATTTTACTCCGAGAGGCTTGCTGCTCTCACTCCTGGATTTGCTGGAGCAGACATAGCAAACGTCTGCAATGAAGCTGCCTTGATTGCTGCAAGAAATGAGAGTCCAAAGATAACCATGAAACATTTTGAAGCGGCTATAGACAGGGTGATAGGCGGCCTAGAAAAGAAGAACAAGGTTATTAGCAAGTTGGAAAGACGCACTGTTGCTTACCATGAATCTGGTCATGCTGTTGCGGGTTGGTTCCTGGAACATGCAGAACCTTTGCTTAAAGTAACTATTGTTCCACGTGGCACTGCAGCACTGGGGTTTGCTCAGTATGTTCCGAATGAAAATCTTCTTATGACCAAAGAGCAGCTTTTTGATATGACATGCATGACCCTTGGTGGCCGAGCTGCTGAGCAGGTTTTGTTGGGGAAGATTTCAACTGGAGCACAGAACGACTTGGAGAAAGTGACCAAAATGACTTATGCTCAAGTTGCAGTGTATGGCTTCAGCGACAAGGTAGGTCTTCTTTCGTTTCCTCAGAGGGAGGATTCATTTGAGATGACCAAGCCATACAGTAGCAAAACCGGTGCAATAATTGACAGTGAAGTTAGAGAATGGGTGGGTAAAGCATACTTCCGCACCGTCCAGTTGATAGAGGAGCACAAAGAGCATGTAGGACAGATTGCAGAGCTGTTGCTGGAAAAGGAAGTCCTTCACCAAGAGGATTTGATTCGAGTACTGGGAGAACGCCCATTCAAGTCGAATGAGCCCACAAACTATGACAGATTCAAGGAAGGGTTTCAAGAAGAAGATAAGGAGGCTAAAGAGACCAATGCGAAGGATGGTCGGTCGCCACCCATTCAACCGGATGTCGTTGCGCCTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA FtsH_ext Peptidase_M41

Protein Analysis

816

Amino Acids

90.13

Weight (kDa)

8.82

Isoelectric Point (pI)

38.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FtsH_ext PF06480 150 - 257 1.1e-09 FtsH Extracellular
AAA PF00004 368 - 500 1.1e-42 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 529 - 569 2.1e-13 AAA+ lid domain
Peptidase_M41 PF01434 584 - 765 1.3e-64 Peptidase family M41
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 189
AarI CACCTGC 1 cut(s) 377
Acc16I TGCGCA 1 cut(s) 1267
Acc36I ACCTGC 2 cut(s) 377, 1966
AccB1I GGYRCC 1 cut(s) 1168
AccI GTMKAC 1 cut(s) 125
AccII CGCG 1 cut(s) 615
AciI CCGC 8 cut(s) 116, 156, 205, 1001, 1706, 1728, 1808, 2197
AclWI GGATC 2 cut(s) 35, 1569
AcoI YGGCCR 4 cut(s) 916, 1306, 1339, 1960
AcsI RAATTY 5 cut(s) 50, 261, 336, 521, 1086
AcuI CTGAAG 2 cut(s) 191, 2050
AcvI CACGTG 1 cut(s) 1862
AfaI GTAC 4 cut(s) 76, 1360, 1419, 2298
AfiI CCNNNNNNNGG 5 cut(s) 347, 656, 671, 893, 1124
AflIII ACRYGT 1 cut(s) 225
AgeI ACCGGT 1 cut(s) 2144
AjnI CCWGG 7 cut(s) 405, 544, 901, 1117, 1468, 1606, 1818
AjuI GAANNNNNNNTTGG 2 cut(s) 1197, 1229
Alw21I GWGCWC 5 cut(s) 608, 1044, 1102, 2008, 2223
Alw26I GTCTC 1 cut(s) 2381
Alw44I GTGCAC 1 cut(s) 1040
AlwI GGATC 2 cut(s) 35, 1569
Ama87I CYCGRG 1 cut(s) 290
ApaLI GTGCAC 1 cut(s) 1040
ApeKI GCWGC 9 cut(s) 97, 100, 130, 1595, 1649, 1661, 1871, 1927, 1967
ApoI RAATTY 5 cut(s) 50, 261, 336, 521, 1086
ArsI GACNNNNNNTTYG 2 cut(s) 224, 256
AsiGI ACCGGT 1 cut(s) 2144
Asp700I GAANNNNTTC 4 cut(s) 621, 1908, 2271, 2358
AspLEI GCGC 2 cut(s) 1268, 2440
AspS9I GGNCC 5 cut(s) 287, 836, 899, 1112, 1223
AsuC2I CCSGG 1 cut(s) 1310
AsuHPI GGTGA 5 cut(s) 22, 362, 1169, 1732, 2267
AsuII TTCGAA 3 cut(s) 419, 726, 1237
AvaI CYCGRG 1 cut(s) 290
AvaII GGWCC 3 cut(s) 287, 899, 1223
BaeGI GKGCMC 1 cut(s) 1044
BalI TGGCCA 1 cut(s) 1341
BanI GGYRCC 1 cut(s) 1168
BanII GRGCYC 4 cut(s) 380, 608, 862, 2331
BarI GAAGNNNNNNTAC 2 cut(s) 287, 319
BbrPI CACGTG 1 cut(s) 1862
BbsI GAAGAC 1 cut(s) 2075
Bbv12I GWGCWC 5 cut(s) 608, 1044, 1102, 2008, 2223
BbvI GCAGC 9 cut(s) 84, 87, 117, 1582, 1636, 1648, 1883, 1939, 1954
BccI CCATC 3 cut(s) 146, 1382, 2396
BcgI CGANNNNNNTGC 4 cut(s) 210, 244, 1574, 1608
BciT130I CCWGG 7 cut(s) 407, 546, 903, 1119, 1470, 1608, 1820
BcnI CCSGG 1 cut(s) 1310
BcoDI GTCTC 1 cut(s) 2381
BfaI CTAG 2 cut(s) 258, 1733
BfmI CTRYAG 2 cut(s) 1710, 1869
BfuAI ACCTGC 2 cut(s) 377, 1966
BlpI GCTNAGC 2 cut(s) 1017, 1971
BmcAI AGTACT 2 cut(s) 1360, 2298
Bme1390I CCNGG 8 cut(s) 407, 546, 903, 1119, 1310, 1470, 1608, 1820
Bme18I GGWCC 3 cut(s) 287, 899, 1223
BmeT110I CYCGRG 1 cut(s) 290
BmgT120I GGNCC 5 cut(s) 287, 836, 899, 1112, 1223
BmiI GGNNCC 5 cut(s) 289, 859, 1170, 1399, 1817
BmrFI CCNGG 8 cut(s) 407, 546, 903, 1119, 1310, 1470, 1608, 1820
BmrI ACTGGG 4 cut(s) 679, 1266, 1886, 2309
BmsI GCATC 4 cut(s) 24, 806, 1288, 1580
BmuI ACTGGG 4 cut(s) 679, 1266, 1886, 2309
BpiI GAAGAC 1 cut(s) 2075
BpmI CTGGAG 4 cut(s) 262, 1428, 1638, 2022
Bpu1102I GCTNAGC 2 cut(s) 1017, 1971
Bpu14I TTCGAA 3 cut(s) 419, 726, 1237
BpuEI CTTGAG 5 cut(s) 364, 732, 1483, 1538, 2031
BpuMI CCSGG 1 cut(s) 1310
BsaAI YACGTR 1 cut(s) 1862
BsaI GGTCTC 1 cut(s) 2381
BsaJI CCNNGG 6 cut(s) 283, 405, 406, 545, 919, 1954
BsaWI WCCGGW 2 cut(s) 2144, 2425
BsaXI ACNNNNNCTCC 2 cut(s) 1401, 1431
Bsc4I CCNNNNNNNGG 5 cut(s) 347, 656, 671, 893, 1124
Bse118I RCCGGY 2 cut(s) 1473, 2144
Bse1I ACTGG 6 cut(s) 685, 1272, 1881, 2005, 2206, 2304
Bse3DI GCAATG 2 cut(s) 145, 1648
BseBI CCWGG 7 cut(s) 407, 546, 903, 1119, 1470, 1608, 1820
BseDI CCNNGG 6 cut(s) 283, 405, 406, 545, 919, 1954
BseGI GGATG 4 cut(s) 797, 1393, 2407, 2434
BseLI CCNNNNNNNGG 5 cut(s) 347, 656, 671, 893, 1124
BseMI GCAATG 2 cut(s) 145, 1648
BseMII CTCAG 4 cut(s) 1535, 1901, 1962, 2111
BseNI ACTGG 6 cut(s) 685, 1272, 1881, 2005, 2206, 2304
BseRI GAGGAG 2 cut(s) 616, 2231
BseSI GKGCMC 1 cut(s) 1044
BseXI GCAGC 9 cut(s) 84, 87, 117, 1582, 1636, 1648, 1883, 1939, 1954
BseYI CCCAGC 1 cut(s) 197
BsgI GTGCAG 2 cut(s) 86, 405
Bsh1236I CGCG 1 cut(s) 615
Bsh1285I CGRYCG 1 cut(s) 2411
BshNI GGYRCC 1 cut(s) 1168
BshTI ACCGGT 1 cut(s) 2144
BsiEI CGRYCG 1 cut(s) 2411
BsiHKAI GWGCWC 5 cut(s) 608, 1044, 1102, 2008, 2223
BsiHKCI CYCGRG 1 cut(s) 290
BsiSI CCGG 4 cut(s) 1309, 1474, 2145, 2426
BslFI GGGAC 2 cut(s) 273, 423
BslI CCNNNNNNNGG 5 cut(s) 347, 656, 671, 893, 1124
BsmAI GTCTC 1 cut(s) 2381
BsmFI GGGAC 2 cut(s) 273, 423
BsmI GAATGC 2 cut(s) 406, 973
Bso31I GGTCTC 1 cut(s) 2381
BsoBI CYCGRG 1 cut(s) 290
Bsp119I TTCGAA 3 cut(s) 419, 726, 1237
Bsp1286I GDGCHC 8 cut(s) 380, 608, 862, 1044, 1102, 2008, 2223, 2331
Bsp143I GATC 5 cut(s) 27, 556, 766, 948, 1561
Bsp1720I GCTNAGC 2 cut(s) 1017, 1971
BspACI CCGC 8 cut(s) 116, 156, 205, 1001, 1706, 1728, 1808, 2197
BspCNI CTCAG 4 cut(s) 1536, 1900, 1963, 2110
BspFNI CGCG 1 cut(s) 615
BspHI TCATGA 2 cut(s) 772, 870
BspLI GGNNCC 5 cut(s) 289, 859, 1170, 1399, 1817
BspMAI CTGCAG 1 cut(s) 1873
BspMI ACCTGC 2 cut(s) 377, 1966
BspPI GGATC 2 cut(s) 35, 1569
BspQI GCTCTTC 1 cut(s) 1107
BspT104I TTCGAA 3 cut(s) 419, 726, 1237
BspT107I GGYRCC 1 cut(s) 1168
BspTNI GGTCTC 1 cut(s) 2381
BsrDI GCAATG 2 cut(s) 145, 1648
BsrFI RCCGGY 2 cut(s) 1473, 2144
BsrI ACTGG 6 cut(s) 685, 1272, 1881, 2005, 2206, 2304
BssAI RCCGGY 2 cut(s) 1473, 2144
BssECI CCNNGG 6 cut(s) 283, 405, 406, 545, 919, 1954
BssMI GATC 5 cut(s) 27, 556, 766, 948, 1561
BssT1I CCWWGG 2 cut(s) 283, 1954
Bst2UI CCWGG 7 cut(s) 407, 546, 903, 1119, 1470, 1608, 1820
Bst4CI ACNGT 8 cut(s) 414, 844, 1263, 1484, 1777, 2135, 2162, 2203
Bst6I CTCTTC 1 cut(s) 1107
BstAPI GCANNNNNTGC 1 cut(s) 144
BstBAI YACGTR 1 cut(s) 1862
BstBI TTCGAA 3 cut(s) 419, 726, 1237
BstC8I GCNNGC 5 cut(s) 1003, 1426, 1550, 1593, 2442
BstDEI CTNAG 6 cut(s) 1017, 1059, 1544, 1887, 1971, 2097
BstENI CCTNNNNNAGG 3 cut(s) 654, 669, 1122
BstF5I GGATG 4 cut(s) 797, 1393, 2407, 2434
BstFNI CGCG 1 cut(s) 615
BstHHI GCGC 2 cut(s) 1268, 2440
BstKTI GATC 5 cut(s) 30, 559, 769, 951, 1564
BstMAI GTCTC 1 cut(s) 2381
BstMBI GATC 5 cut(s) 27, 556, 766, 948, 1561
BstMCI CGRYCG 1 cut(s) 2411
BstMWI GCNNNNNNNGC 9 cut(s) 21, 144, 153, 612, 752, 1628, 1658, 2051, 2227
BstNI CCWGG 7 cut(s) 407, 546, 903, 1119, 1470, 1608, 1820
BstNSI RCATGY 4 cut(s) 229, 1829, 1947, 2234
BstSCI CCNGG 8 cut(s) 405, 544, 901, 1117, 1308, 1468, 1606, 1818
BstSFI CTRYAG 2 cut(s) 1710, 1869
BstSLI GKGCMC 1 cut(s) 1044
BstUI CGCG 1 cut(s) 615
BstV1I GCAGC 9 cut(s) 84, 87, 117, 1582, 1636, 1648, 1883, 1939, 1954
BstV2I GAAGAC 1 cut(s) 2075
BstX2I RGATCY 1 cut(s) 1561
BstXI CCANNNNNNTGG 1 cut(s) 1793
BstYI RGATCY 1 cut(s) 1561
BtsCI GGATG 4 cut(s) 797, 1393, 2407, 2434
BtsI GCAGTG 3 cut(s) 1401, 1866, 2061
BtsIMutI CAGTG 8 cut(s) 849, 1268, 1401, 1773, 1866, 1874, 2061, 2167
BveI ACCTGC 2 cut(s) 377, 1966
Cac8I GCNNGC 5 cut(s) 1003, 1426, 1550, 1593, 2442
CciI TCATGA 2 cut(s) 772, 870
CfoI GCGC 2 cut(s) 1268, 2440
Cfr10I RCCGGY 2 cut(s) 1473, 2144
Cfr13I GGNCC 5 cut(s) 287, 836, 899, 1112, 1223
CseI GACGC 4 cut(s) 30, 136, 1141, 1779
Csp6I GTAC 4 cut(s) 75, 1359, 1418, 2297
CspAI ACCGGT 1 cut(s) 2144
CspCI CAANNNNNGTGG 2 cut(s) 791, 826
CviQI GTAC 4 cut(s) 75, 1359, 1418, 2297
DdeI CTNAG 6 cut(s) 1017, 1059, 1544, 1887, 1971, 2097
DpnI GATC 5 cut(s) 29, 558, 768, 950, 1563
DpnII GATC 5 cut(s) 27, 556, 766, 948, 1561
DraI TTTAAA 2 cut(s) 526, 991
EaeI YGGCCR 4 cut(s) 916, 1306, 1339, 1960
Eam1104I CTCTTC 1 cut(s) 1107
EarI CTCTTC 1 cut(s) 1107
Ecl136II GAGCTC 1 cut(s) 606
Eco130I CCWWGG 2 cut(s) 283, 1954
Eco147I AGGCCT 1 cut(s) 1438
Eco24I GRGCYC 4 cut(s) 380, 608, 862, 2331
Eco31I GGTCTC 1 cut(s) 2381
Eco47I GGWCC 3 cut(s) 287, 899, 1223
Eco53kI GAGCTC 1 cut(s) 606
Eco57I CTGAAG 2 cut(s) 191, 2050
Eco72I CACGTG 1 cut(s) 1862
Eco88I CYCGRG 1 cut(s) 290
EcoICRI GAGCTC 1 cut(s) 606
EcoNI CCTNNNNNAGG 3 cut(s) 654, 669, 1122
EcoO109I RGGNCCY 1 cut(s) 287
EcoRI GAATTC 1 cut(s) 261
EcoRII CCWGG 7 cut(s) 405, 544, 901, 1117, 1468, 1606, 1818
EcoT14I CCWWGG 2 cut(s) 283, 1954
EcoT22I ATGCAT 2 cut(s) 799, 1949
EcoT38I GRGCYC 4 cut(s) 380, 608, 862, 2331
ErhI CCWWGG 2 cut(s) 283, 1954
FalI AAGNNNNNCTT 8 cut(s) 423, 455, 971, 1003, 1086, 1118, 1914, 1946
FaqI GGGAC 2 cut(s) 273, 423
FauI CCCGC 2 cut(s) 994, 1801
FblI GTMKAC 1 cut(s) 125
FokI GGATG 4 cut(s) 784, 1400, 2414, 2441
FriOI GRGCYC 4 cut(s) 380, 608, 862, 2331
FspAI RTGCGCAY 1 cut(s) 1267
FspBI CTAG 2 cut(s) 258, 1733
FspI TGCGCA 1 cut(s) 1267
GlaI GCGC 2 cut(s) 1267, 2439
GsaI CCCAGC 1 cut(s) 201
GsuI CTGGAG 4 cut(s) 262, 1428, 1638, 2022
HapII CCGG 4 cut(s) 1309, 1474, 2145, 2426
HgaI GACGC 4 cut(s) 30, 136, 1141, 1779
HhaI GCGC 2 cut(s) 1268, 2440
Hin6I GCGC 2 cut(s) 1266, 2438
HinP1I GCGC 2 cut(s) 1266, 2438
HincII GTYRAC 1 cut(s) 126
HindII GTYRAC 1 cut(s) 126
HindIII AAGCTT 2 cut(s) 753, 1240
HpaII CCGG 4 cut(s) 1309, 1474, 2145, 2426
HphI GGTGA 5 cut(s) 22, 362, 1169, 1732, 2267
Hpy166II GTNNAC 4 cut(s) 126, 353, 985, 1042
Hpy8I GTNNAC 4 cut(s) 126, 353, 985, 1042
Hpy99I CGWCG 2 cut(s) 130, 270
HpyAV CCTTC 8 cut(s) 166, 325, 496, 665, 675, 2282, 2348, 2392
HpyCH4III ACNGT 8 cut(s) 414, 844, 1263, 1484, 1777, 2135, 2162, 2203
HpyCH4IV ACGT 2 cut(s) 1635, 1861
HpyF10VI GCNNNNNNNGC 9 cut(s) 21, 144, 153, 612, 752, 1628, 1658, 2051, 2227
HpyF3I CTNAG 6 cut(s) 1017, 1059, 1544, 1887, 1971, 2097
HpySE526I ACGT 2 cut(s) 1635, 1861
HspAI GCGC 2 cut(s) 1266, 2438
Kzo9I GATC 5 cut(s) 27, 556, 766, 948, 1561
LguI GCTCTTC 1 cut(s) 1107
LmnI GCTCC 9 cut(s) 194, 448, 603, 611, 857, 1079, 1619, 2003, 2218
Lsp1109I GCAGC 9 cut(s) 84, 87, 117, 1582, 1636, 1648, 1883, 1939, 1954
LweI GCATC 4 cut(s) 24, 806, 1288, 1580
MaeI CTAG 2 cut(s) 258, 1733
MaeII ACGT 2 cut(s) 1635, 1861
MaeIII GTNAC 5 cut(s) 469, 679, 1520, 1846, 2026
MalI GATC 5 cut(s) 29, 558, 768, 950, 1563
MboI GATC 5 cut(s) 27, 556, 766, 948, 1561
MboII GAAGA 9 cut(s) 307, 740, 1094, 1753, 1901, 2002, 2075, 2378, 2381
MfeI CAATTG 2 cut(s) 1302, 1370
MflI RGATCY 1 cut(s) 1561
MhlI GDGCHC 8 cut(s) 380, 608, 862, 1044, 1102, 2008, 2223, 2331
MlsI TGGCCA 1 cut(s) 1341
MluNI TGGCCA 1 cut(s) 1341
MlyI GAGTC 2 cut(s) 374, 1684
MmeI TCCRAC 3 cut(s) 717, 939, 1743
Mox20I TGGCCA 1 cut(s) 1341
Mph1103I ATGCAT 2 cut(s) 799, 1949
MroXI GAANNNNTTC 4 cut(s) 621, 1908, 2271, 2358
MscI TGGCCA 1 cut(s) 1341
MseI TTAA 8 cut(s) 449, 486, 525, 654, 702, 827, 990, 1842
MslI CAYNNNNRTG 1 cut(s) 630
Msp20I TGGCCA 1 cut(s) 1341
MspI CCGG 4 cut(s) 1309, 1474, 2145, 2426
MspR9I CCNGG 8 cut(s) 407, 546, 903, 1119, 1310, 1470, 1608, 1820
MunI CAATTG 2 cut(s) 1302, 1370
Mva1269I GAATGC 2 cut(s) 406, 973
MvaI CCWGG 7 cut(s) 407, 546, 903, 1119, 1470, 1608, 1820
MvnI CGCG 1 cut(s) 615
MwoI GCNNNNNNNGC 9 cut(s) 21, 144, 153, 612, 752, 1628, 1658, 2051, 2227
NciI CCSGG 1 cut(s) 1310
NdeII GATC 5 cut(s) 27, 556, 766, 948, 1561
NlaIV GGNNCC 5 cut(s) 289, 859, 1170, 1399, 1817
NmeAIII GCCGAG 4 cut(s) 17, 114, 944, 1988
NmuCI GTSAC 2 cut(s) 469, 2026
NsbI TGCGCA 1 cut(s) 1267
NsiI ATGCAT 2 cut(s) 799, 1949
NspI RCATGY 4 cut(s) 229, 1829, 1947, 2234
NspV TTCGAA 3 cut(s) 419, 726, 1237
PagI TCATGA 2 cut(s) 772, 870
PaqCI CACCTGC 1 cut(s) 377
PasI CCCWGGG 1 cut(s) 406
PceI AGGCCT 1 cut(s) 1438
PciI ACATGT 1 cut(s) 225
PciSI GCTCTTC 1 cut(s) 1107
PctI GAATGC 2 cut(s) 406, 973
PdmI GAANNNNTTC 4 cut(s) 621, 1908, 2271, 2358
PfoI TCCNGGA 3 cut(s) 1117, 1606, 1818
PinAI ACCGGT 1 cut(s) 2144
PleI GAGTC 2 cut(s) 373, 1683
PmaCI CACGTG 1 cut(s) 1862
PmlI CACGTG 1 cut(s) 1862
PpsI GAGTC 2 cut(s) 373, 1683
Ppu21I YACGTR 1 cut(s) 1862
PpuMI RGGWCCY 1 cut(s) 287
PscI ACATGT 1 cut(s) 225
PsiI TTATAA 1 cut(s) 189
Psp124BI GAGCTC 1 cut(s) 608
Psp5II RGGWCCY 1 cut(s) 287
Psp6I CCWGG 7 cut(s) 405, 544, 901, 1117, 1468, 1606, 1818
PspCI CACGTG 1 cut(s) 1862
PspFI CCCAGC 1 cut(s) 197
PspGI CCWGG 7 cut(s) 405, 544, 901, 1117, 1468, 1606, 1818
PspN4I GGNNCC 5 cut(s) 289, 859, 1170, 1399, 1817
PspPI GGNCC 5 cut(s) 287, 836, 899, 1112, 1223
PspPPI RGGWCCY 1 cut(s) 287
PsrI GAACNNNNNNTAC 2 cut(s) 1839, 1871
PstI CTGCAG 1 cut(s) 1873
PsuI RGATCY 1 cut(s) 1561
RsaI GTAC 4 cut(s) 76, 1360, 1419, 2298
RsaNI GTAC 4 cut(s) 75, 1359, 1418, 2297
RseI CAYNNNNRTG 1 cut(s) 630
SacI GAGCTC 1 cut(s) 608
SalI GTCGAC 1 cut(s) 124
SapI GCTCTTC 1 cut(s) 1107
SaqAI TTAA 8 cut(s) 449, 486, 525, 654, 702, 827, 990, 1842
Sau3AI GATC 5 cut(s) 27, 556, 766, 948, 1561
Sau96I GGNCC 5 cut(s) 287, 836, 899, 1112, 1223
ScaI AGTACT 2 cut(s) 1360, 2298
SchI GAGTC 2 cut(s) 374, 1684
ScrFI CCNGG 8 cut(s) 407, 546, 903, 1119, 1310, 1470, 1608, 1820
SduI GDGCHC 8 cut(s) 380, 608, 862, 1044, 1102, 2008, 2223, 2331
SfaNI GCATC 4 cut(s) 24, 806, 1288, 1580
SfcI CTRYAG 2 cut(s) 1710, 1869
SfuI TTCGAA 3 cut(s) 419, 726, 1237
SinI GGWCC 3 cut(s) 287, 899, 1223
SmiMI CAYNNNNRTG 1 cut(s) 630
SmlI CTYRAG 6 cut(s) 10, 379, 747, 1462, 1553, 2046
SmoI CTYRAG 6 cut(s) 10, 379, 747, 1462, 1553, 2046
SseBI AGGCCT 1 cut(s) 1438
SsiI CCGC 8 cut(s) 116, 156, 205, 1001, 1706, 1728, 1808, 2197
SspI AATATT 2 cut(s) 695, 1281
SspMI CTAG 2 cut(s) 258, 1733
SstI GAGCTC 1 cut(s) 608
StuI AGGCCT 1 cut(s) 1438
StyD4I CCNGG 8 cut(s) 405, 544, 901, 1117, 1308, 1468, 1606, 1818
StyI CCWWGG 2 cut(s) 283, 1954
TaaI ACNGT 8 cut(s) 414, 844, 1263, 1484, 1777, 2135, 2162, 2203
TaiI ACGT 2 cut(s) 1638, 1864
TaqI TCGA 8 cut(s) 125, 230, 419, 555, 726, 1237, 2293, 2321
TaqII GACCGA 1 cut(s) 2397
TatI WGTACW 3 cut(s) 1358, 1417, 2296
TauI GCSGC 4 cut(s) 159, 208, 1709, 1731
Tru1I TTAA 8 cut(s) 449, 486, 525, 654, 702, 827, 990, 1842
Tru9I TTAA 8 cut(s) 449, 486, 525, 654, 702, 827, 990, 1842
TscAI CASTG 8 cut(s) 849, 1268, 1408, 1780, 1873, 1881, 2061, 2167
TseFI GTSAC 2 cut(s) 469, 2026
TseI GCWGC 9 cut(s) 97, 100, 130, 1595, 1649, 1661, 1871, 1927, 1967
Tsp45I GTSAC 2 cut(s) 469, 2026
TspDTI ATGAA 8 cut(s) 445, 614, 859, 1659, 1706, 1803, 1917, 2100
TspGWI ACGGA 2 cut(s) 377, 1215
TspRI CASTG 8 cut(s) 849, 1268, 1408, 1780, 1873, 1881, 2061, 2167
VneI GTGCAC 1 cut(s) 1040
VpaK11BI GGWCC 3 cut(s) 287, 899, 1223
XagI CCTNNNNNAGG 3 cut(s) 654, 669, 1122
XapI RAATTY 5 cut(s) 50, 261, 336, 521, 1086
XceI RCATGY 4 cut(s) 229, 1829, 1947, 2234
XmiI GTMKAC 1 cut(s) 125
XmnI GAANNNNTTC 4 cut(s) 621, 1908, 2271, 2358
XspI CTAG 2 cut(s) 258, 1733
ZrmI AGTACT 2 cut(s) 1360, 2298
Zsp2I ATGCAT 2 cut(s) 799, 1949
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.