MD03G1230600.v1.1

squamosa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002905
Physical Location & Seq
Forward (+)
31586432 .. 31588342
1911 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1230600.v1.1.491

Sequence Viewer

Length: 576 bp
ATGGAGGGCAAGAACTTTGAAGGAAGGCAAACATGGAAAGAGAAGGCGAAGAAGGATGTCGAGGAAGTAGAGGATGACTACTCGGATGAAGAAGAGACCAGTGGTGGTGGCGGAGGGTTGATGCTGAGATTTGAAGAAAATGAGAGGCAGAAGAAAGCCGCTGCAGCTGGAAGAAGAGGATCTGGTGGCGGAGGTGGAGGTGGAGGGGGGATAGCGCAACCGTCTTGTCAAGTGGAGAGGTGTGGGGCTGATTTGGTTGATGCAAAGAGGTACTACCGCCGGCATAAGGTTTGTGAGTTTCATTCCAAGGCAGCTGTTGTGATCGTTTCCGGGATTCAACAGCGGTTTTGCCAGCAATGCAGCAGGTTCCATGAGCTAATTGAATTTGACGAAGCAAAGAGAAGCTGTCGCCGGCGTTTGGCAGGACACAATGAACGACGCCGCAAGAGCTCAGGCGAACCTTACGGAGAAGGATCGAGCCGGAGAGGGGTTGGTCACCAGTACAAAGAAAGTCAGACTGGATATCAGATCACACCCCCAGGAAACTCTTCTTCCAAGCATTGCCAGATCAGATAA

Protein Analysis

192

Amino Acids

21.23

Weight (kDa)

9.08

Isoelectric Point (pI)

66.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 76 - 149 6.3e-33 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 354
AciI CCGC 6 cut(s) 111, 159, 189, 277, 343, 442
AclWI GGATC 2 cut(s) 187, 481
AcsI RAATTY 1 cut(s) 383
AcyI GRCGYC 1 cut(s) 439
AfaI GTAC 2 cut(s) 272, 503
AfiI CCNNNNNNNGG 3 cut(s) 286, 418, 487
AgsI TTSAA 4 cut(s) 20, 134, 338, 383
AjnI CCWGG 1 cut(s) 538
AluBI AGCT 5 cut(s) 167, 314, 376, 405, 450
AluI AGCT 5 cut(s) 167, 314, 376, 405, 450
Alw21I GWGCWC 1 cut(s) 452
Alw26I GTCTC 1 cut(s) 89
AlwI GGATC 2 cut(s) 187, 481
ApeKI GCWGC 4 cut(s) 161, 164, 311, 360
ApoI RAATTY 1 cut(s) 383
Asp700I GAANNNNTTC 1 cut(s) 547
AspLEI GCGC 1 cut(s) 217
AsuC2I CCSGG 1 cut(s) 331
AsuHPI GGTGA 1 cut(s) 488
BanII GRGCYC 1 cut(s) 452
Bbv12I GWGCWC 1 cut(s) 452
BbvI GCAGC 4 cut(s) 148, 176, 323, 372
BciT130I CCWGG 1 cut(s) 540
BcnI CCSGG 1 cut(s) 331
BcoDI GTCTC 1 cut(s) 89
BfmI CTRYAG 1 cut(s) 162
BfuAI ACCTGC 1 cut(s) 354
BisI GCNGC 6 cut(s) 159, 162, 165, 312, 361, 442
BlsI GCNGC 6 cut(s) 160, 163, 166, 313, 362, 443
Bme1390I CCNGG 2 cut(s) 331, 540
BmiI GGNNCC 1 cut(s) 368
BmrFI CCNGG 2 cut(s) 331, 540
BmsI GCATC 2 cut(s) 111, 250
Bpu10I CCTNAGC 1 cut(s) 451
BpuMI CCSGG 1 cut(s) 331
BsaHI GRCGYC 1 cut(s) 439
BsaI GGTCTC 1 cut(s) 89
BsaJI CCNNGG 2 cut(s) 306, 538
Bsc4I CCNNNNNNNGG 3 cut(s) 286, 418, 487
Bse118I RCCGGY 2 cut(s) 279, 411
Bse1I ACTGG 3 cut(s) 99, 499, 523
Bse3DI GCAATG 2 cut(s) 362, 559
BseBI CCWGG 1 cut(s) 540
BseDI CCNNGG 2 cut(s) 306, 538
BseGI GGATG 3 cut(s) 61, 79, 91
BseLI CCNNNNNNNGG 3 cut(s) 286, 418, 487
BseMI GCAATG 2 cut(s) 362, 559
BseMII CTCAG 2 cut(s) 116, 465
BseNI ACTGG 3 cut(s) 99, 499, 523
BseXI GCAGC 4 cut(s) 148, 176, 323, 372
BsiHKAI GWGCWC 1 cut(s) 452
BsiSI CCGG 4 cut(s) 280, 330, 412, 481
BslI CCNNNNNNNGG 3 cut(s) 286, 418, 487
BsmAI GTCTC 1 cut(s) 89
Bso31I GGTCTC 1 cut(s) 89
Bsp1286I GDGCHC 1 cut(s) 452
Bsp143I GATC 5 cut(s) 179, 321, 473, 528, 567
BspACI CCGC 6 cut(s) 111, 159, 189, 277, 343, 442
BspCNI CTCAG 2 cut(s) 117, 464
BspLI GGNNCC 1 cut(s) 368
BspMAI CTGCAG 1 cut(s) 166
BspMI ACCTGC 1 cut(s) 354
BspPI GGATC 2 cut(s) 187, 481
BspTNI GGTCTC 1 cut(s) 89
BsrDI GCAATG 2 cut(s) 362, 559
BsrFI RCCGGY 2 cut(s) 279, 411
BsrI ACTGG 3 cut(s) 99, 499, 523
BssAI RCCGGY 2 cut(s) 279, 411
BssECI CCNNGG 2 cut(s) 306, 538
BssMI GATC 5 cut(s) 179, 321, 473, 528, 567
BssNI GRCGYC 1 cut(s) 439
BssT1I CCWWGG 1 cut(s) 306
Bst2UI CCWGG 1 cut(s) 540
Bst4CI ACNGT 1 cut(s) 222
Bst6I CTCTTC 3 cut(s) 87, 169, 553
BstACI GRCGYC 1 cut(s) 439
BstC8I GCNNGC 3 cut(s) 281, 353, 413
BstDEI CTNAG 2 cut(s) 125, 451
BstEII GGTNACC 1 cut(s) 494
BstF5I GGATG 3 cut(s) 61, 79, 91
BstHHI GCGC 1 cut(s) 217
BstKTI GATC 5 cut(s) 182, 324, 476, 531, 570
BstMAI GTCTC 1 cut(s) 89
BstMBI GATC 5 cut(s) 179, 321, 473, 528, 567
BstMWI GCNNNNNNNGC 3 cut(s) 164, 357, 447
BstNI CCWGG 1 cut(s) 540
BstPI GGTNACC 1 cut(s) 494
BstSCI CCNGG 2 cut(s) 329, 538
BstSFI CTRYAG 1 cut(s) 162
BstV1I GCAGC 4 cut(s) 148, 176, 323, 372
BstX2I RGATCY 1 cut(s) 179
BstYI RGATCY 1 cut(s) 179
BtsCI GGATG 3 cut(s) 61, 79, 91
BtsIMutI CAGTG 1 cut(s) 106
BveI ACCTGC 1 cut(s) 354
Cac8I GCNNGC 3 cut(s) 281, 353, 413
CfoI GCGC 1 cut(s) 217
Cfr10I RCCGGY 2 cut(s) 279, 411
CseI GACGC 1 cut(s) 447
Csp6I GTAC 2 cut(s) 271, 502
CviAII CATG 2 cut(s) 33, 371
CviJI RGCY 8 cut(s) 158, 167, 248, 314, 376, 405, 450, 480
CviKI_1 RGCY 8 cut(s) 158, 167, 248, 314, 376, 405, 450, 480
CviQI GTAC 2 cut(s) 271, 502
DdeI CTNAG 2 cut(s) 125, 451
DpnI GATC 5 cut(s) 181, 323, 475, 530, 569
DpnII GATC 5 cut(s) 179, 321, 473, 528, 567
Eam1104I CTCTTC 3 cut(s) 87, 169, 553
EarI CTCTTC 3 cut(s) 87, 169, 553
EciI GGCGGA 2 cut(s) 126, 204
Ecl136II GAGCTC 1 cut(s) 450
Eco130I CCWWGG 1 cut(s) 306
Eco24I GRGCYC 1 cut(s) 452
Eco31I GGTCTC 1 cut(s) 89
Eco32I GATATC 1 cut(s) 524
Eco53kI GAGCTC 1 cut(s) 450
Eco91I GGTNACC 1 cut(s) 494
EcoICRI GAGCTC 1 cut(s) 450
EcoO65I GGTNACC 1 cut(s) 494
EcoRII CCWGG 1 cut(s) 538
EcoRV GATATC 1 cut(s) 524
EcoT14I CCWWGG 1 cut(s) 306
EcoT38I GRGCYC 1 cut(s) 452
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 2 cut(s) 36, 374
FaiI YATR 3 cut(s) 34, 285, 372
FatI CATG 2 cut(s) 32, 370
Fnu4HI GCNGC 6 cut(s) 159, 162, 165, 312, 361, 442
FokI GGATG 3 cut(s) 68, 86, 98
FriOI GRGCYC 1 cut(s) 452
Fsp4HI GCNGC 6 cut(s) 159, 162, 165, 312, 361, 442
GlaI GCGC 1 cut(s) 216
GluI GCNGC 6 cut(s) 159, 162, 165, 312, 361, 442
HapII CCGG 4 cut(s) 280, 330, 412, 481
HgaI GACGC 1 cut(s) 447
HhaI GCGC 1 cut(s) 217
Hin1I GRCGYC 1 cut(s) 439
Hin1II CATG 2 cut(s) 36, 374
Hin6I GCGC 1 cut(s) 215
HinP1I GCGC 1 cut(s) 215
HinfI GANTC 1 cut(s) 334
HpaII CCGG 4 cut(s) 280, 330, 412, 481
HphI GGTGA 1 cut(s) 488
Hpy188I TCNGA 4 cut(s) 85, 516, 528, 572
Hpy99I CGWCG 1 cut(s) 441
HpyAV CCTTC 5 cut(s) 14, 18, 37, 46, 464
HpyCH4III ACNGT 1 cut(s) 222
HpyCH4V TGCA 3 cut(s) 164, 263, 360
HpyF10VI GCNNNNNNNGC 3 cut(s) 164, 357, 447
HpyF3I CTNAG 2 cut(s) 125, 451
Hsp92I GRCGYC 1 cut(s) 439
Hsp92II CATG 2 cut(s) 36, 374
HspAI GCGC 1 cut(s) 215
KroI GCCGGC 2 cut(s) 279, 411
KroNI GCCGGC 2 cut(s) 281, 413
Kzo9I GATC 5 cut(s) 179, 321, 473, 528, 567
Lsp1109I GCAGC 4 cut(s) 148, 176, 323, 372
LweI GCATC 2 cut(s) 111, 250
MaeIII GTNAC 1 cut(s) 494
MalI GATC 5 cut(s) 181, 323, 475, 530, 569
MboI GATC 5 cut(s) 179, 321, 473, 528, 567
MboII GAAGA 9 cut(s) 61, 101, 104, 146, 163, 183, 186, 540, 543
MflI RGATCY 1 cut(s) 179
MhlI GDGCHC 1 cut(s) 452
MluCI AATT 2 cut(s) 378, 383
MreI CGCCGGCG 1 cut(s) 411
MroNI GCCGGC 2 cut(s) 279, 411
MroXI GAANNNNTTC 1 cut(s) 547
MspA1I CMGCKG 4 cut(s) 161, 167, 314, 343
MspI CCGG 4 cut(s) 280, 330, 412, 481
MspR9I CCNGG 2 cut(s) 331, 540
MvaI CCWGG 1 cut(s) 540
MwoI GCNNNNNNNGC 3 cut(s) 164, 357, 447
NaeI GCCGGC 2 cut(s) 281, 413
NciI CCSGG 1 cut(s) 331
NdeII GATC 5 cut(s) 179, 321, 473, 528, 567
NgoMIV GCCGGC 2 cut(s) 279, 411
NlaIII CATG 2 cut(s) 36, 374
NlaIV GGNNCC 1 cut(s) 368
NmuCI GTSAC 1 cut(s) 494
PdiI GCCGGC 2 cut(s) 281, 413
PdmI GAANNNNTTC 1 cut(s) 547
PfeI GAWTC 1 cut(s) 334
PfoI TCCNGGA 1 cut(s) 329
PkrI GCNGC 6 cut(s) 160, 163, 166, 313, 362, 443
Psp124BI GAGCTC 1 cut(s) 452
Psp6I CCWGG 1 cut(s) 538
PspEI GGTNACC 1 cut(s) 494
PspGI CCWGG 1 cut(s) 538
PspN4I GGNNCC 1 cut(s) 368
PstI CTGCAG 1 cut(s) 166
PsuI RGATCY 1 cut(s) 179
PvuII CAGCTG 2 cut(s) 167, 314
RsaI GTAC 2 cut(s) 272, 503
RsaNI GTAC 2 cut(s) 271, 502
SacI GAGCTC 1 cut(s) 452
SatI GCNGC 6 cut(s) 159, 162, 165, 312, 361, 442
Sau3AI GATC 5 cut(s) 179, 321, 473, 528, 567
ScrFI CCNGG 2 cut(s) 331, 540
SduI GDGCHC 1 cut(s) 452
SfaNI GCATC 2 cut(s) 111, 250
SfcI CTRYAG 1 cut(s) 162
SgrAI CRCCGGYG 1 cut(s) 411
Sse9I AATT 2 cut(s) 378, 383
SsiI CCGC 6 cut(s) 111, 159, 189, 277, 343, 442
SstI GAGCTC 1 cut(s) 452
StyD4I CCNGG 2 cut(s) 329, 538
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 1 cut(s) 222
TaqI TCGA 2 cut(s) 60, 476
TasI AATT 2 cut(s) 378, 383
TatI WGTACW 1 cut(s) 501
TauI GCSGC 2 cut(s) 161, 444
TfiI GAWTC 1 cut(s) 334
TscAI CASTG 1 cut(s) 106
TseFI GTSAC 1 cut(s) 494
TseI GCWGC 4 cut(s) 161, 164, 311, 360
Tsp45I GTSAC 1 cut(s) 494
TspDTI ATGAA 3 cut(s) 102, 290, 447
TspGWI ACGGA 1 cut(s) 480
TspRI CASTG 1 cut(s) 106
XapI RAATTY 1 cut(s) 383
XmnI GAANNNNTTC 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.