Rw5G020820

squamosa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002905
Physical Location & Seq
Reverse (-)
27583698 .. 27584942
1245 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G020820.1

Sequence Viewer

Length: 588 bp
ATGGACAGGCAAACTTGGAAAGAAAAGATGCTCAACAAGAATGCTGATGAAGTTGAAGATGACTCGGACGAAGATGAGACTTGTGGCCACGGTAGCGGGTTGATCAGCATAAGATTTGAAGAAAATGAGAAGCACAAGGTCAAGGCAGCTGGGAGTACTGGAAAAAAATCAGGATCCAGTAGCGGAGGTGGAGGTGGAGCGTCTTCAGCACCGAGTTGTCAAGCAGATAGGTGTGGTGCTGATTTGGGTGATGCAAAGATCTACCATCGCCGCCATAAGGTTTGTGAGTTTCACTCCAAGGCAGCGATTGTGGTCGTCTCCGGGGCTCGACAGAGGTTTTGTCAGCAATGCAGCAGGTTCCATGAGCTAGCTGAGTTTGATGAAGCAAAGAGAAGCTGTCGCCGACGTTTGGCAGGTCACAATGAGAGGCGCCGCAAGAGCTCAGGTGAACCTTATGGAGAGGGCTCAAGCCGCCGGGGTATCAGTCATCAATACCGATCAGAAAGTACTCAGGCTACTACTAAATATCATATGACATCCCCAGCCCCAGGAAACACTGCTAATTCCAAGCATTTCCAGATCAGCTAA

Protein Analysis

195

Amino Acids

21.45

Weight (kDa)

9.1

Isoelectric Point (pI)

58.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 73 - 146 5.4e-32 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 345, 404
AccB1I GGYRCC 1 cut(s) 429
AciI CCGC 5 cut(s) 96, 183, 271, 433, 472
AclWI GGATC 2 cut(s) 168, 181
AcoI YGGCCR 1 cut(s) 85
AcuI CTGAAG 1 cut(s) 189
AcyI GRCGYC 1 cut(s) 430
AfaI GTAC 2 cut(s) 157, 508
AfiI CCNNNNNNNGG 3 cut(s) 277, 409, 548
AgsI TTSAA 2 cut(s) 56, 119
AjnI CCWGG 1 cut(s) 547
AluBI AGCT 6 cut(s) 149, 367, 371, 396, 441, 585
AluI AGCT 6 cut(s) 149, 367, 371, 396, 441, 585
Alw21I GWGCWC 1 cut(s) 443
Alw26I GTCTC 2 cut(s) 71, 322
AlwI GGATC 2 cut(s) 168, 181
AoxI GGCC 1 cut(s) 85
ApeKI GCWGC 3 cut(s) 146, 302, 351
ArsI GACNNNNNNTTYG 2 cut(s) 321, 353
AspLEI GCGC 1 cut(s) 432
AsuC2I CCSGG 2 cut(s) 322, 476
AsuHPI GGTGA 2 cut(s) 260, 458
AsuNHI GCTAGC 1 cut(s) 367
BalI TGGCCA 1 cut(s) 87
BamHI GGATCC 1 cut(s) 173
BanI GGYRCC 1 cut(s) 429
BanII GRGCYC 3 cut(s) 328, 443, 467
BbsI GAAGAC 1 cut(s) 195
Bbv12I GWGCWC 1 cut(s) 443
BbvI GCAGC 3 cut(s) 158, 314, 363
BccI CCATC 1 cut(s) 273
BciT130I CCWGG 1 cut(s) 549
BclI TGATCA 1 cut(s) 102
BcnI CCSGG 2 cut(s) 322, 476
BcoDI GTCTC 2 cut(s) 71, 322
BfaI CTAG 1 cut(s) 368
BfoI RGCGCY 1 cut(s) 433
BfuAI ACCTGC 2 cut(s) 345, 404
BglII AGATCT 1 cut(s) 258
BisI GCNGC 6 cut(s) 147, 271, 303, 352, 433, 472
BlsI GCNGC 6 cut(s) 148, 272, 304, 353, 434, 473
BmcAI AGTACT 2 cut(s) 157, 508
Bme1390I CCNGG 3 cut(s) 322, 476, 549
BmiI GGNNCC 3 cut(s) 175, 359, 431
BmrFI CCNGG 3 cut(s) 322, 476, 549
BmsI GCATC 2 cut(s) 18, 241
BmtI GCTAGC 1 cut(s) 371
BpiI GAAGAC 1 cut(s) 195
BplI GAGNNNNNCTC 2 cut(s) 278, 310
Bpu10I CCTNAGC 1 cut(s) 442
BpuEI CTTGAG 1 cut(s) 451
BpuMI CCSGG 2 cut(s) 322, 476
BsaHI GRCGYC 1 cut(s) 430
BsaJI CCNNGG 5 cut(s) 88, 297, 321, 475, 547
Bsc4I CCNNNNNNNGG 3 cut(s) 277, 409, 548
Bse1I ACTGG 2 cut(s) 163, 177
Bse3DI GCAATG 1 cut(s) 353
BseBI CCWGG 1 cut(s) 549
BseDI CCNNGG 5 cut(s) 88, 297, 321, 475, 547
BseGI GGATG 1 cut(s) 536
BseLI CCNNNNNNNGG 3 cut(s) 277, 409, 548
BseMI GCAATG 1 cut(s) 353
BseMII CTCAG 3 cut(s) 363, 456, 524
BseNI ACTGG 2 cut(s) 163, 177
BseXI GCAGC 3 cut(s) 158, 314, 363
BseYI CCCAGC 2 cut(s) 149, 541
BshFI GGCC 1 cut(s) 87
BshNI GGYRCC 1 cut(s) 429
BsiHKAI GWGCWC 1 cut(s) 443
BsiSI CCGG 2 cut(s) 321, 475
BslI CCNNNNNNNGG 3 cut(s) 277, 409, 548
BsmAI GTCTC 2 cut(s) 71, 322
BsmBI CGTCTC 1 cut(s) 322
BsmI GAATGC 1 cut(s) 46
BsnI GGCC 1 cut(s) 87
Bsp1286I GDGCHC 3 cut(s) 328, 443, 467
Bsp143I GATC 5 cut(s) 102, 173, 258, 497, 579
BspACI CCGC 5 cut(s) 96, 183, 271, 433, 472
BspANI GGCC 1 cut(s) 87
BspCNI CTCAG 3 cut(s) 364, 455, 523
BspLI GGNNCC 3 cut(s) 175, 359, 431
BspMI ACCTGC 2 cut(s) 345, 404
BspOI GCTAGC 1 cut(s) 371
BspPI GGATC 2 cut(s) 168, 181
BspT107I GGYRCC 1 cut(s) 429
BsrDI GCAATG 1 cut(s) 353
BsrI ACTGG 2 cut(s) 163, 177
BssECI CCNNGG 5 cut(s) 88, 297, 321, 475, 547
BssMI GATC 5 cut(s) 102, 173, 258, 497, 579
BssNI GRCGYC 1 cut(s) 430
BssT1I CCWWGG 1 cut(s) 297
Bst2UI CCWGG 1 cut(s) 549
Bst4CI ACNGT 1 cut(s) 92
BstACI GRCGYC 1 cut(s) 430
BstC8I GCNNGC 1 cut(s) 369
BstDEI CTNAG 3 cut(s) 372, 442, 510
BstDSI CCRYGG 1 cut(s) 88
BstF5I GGATG 1 cut(s) 536
BstH2I RGCGCY 1 cut(s) 433
BstHHI GCGC 1 cut(s) 432
BstKTI GATC 5 cut(s) 105, 176, 261, 500, 582
BstMAI GTCTC 2 cut(s) 71, 322
BstMBI GATC 5 cut(s) 102, 173, 258, 497, 579
BstMWI GCNNNNNNNGC 4 cut(s) 93, 206, 438, 471
BstNI CCWGG 1 cut(s) 549
BstSCI CCNGG 3 cut(s) 320, 474, 547
BstV1I GCAGC 3 cut(s) 158, 314, 363
BstV2I GAAGAC 1 cut(s) 195
BstX2I RGATCY 2 cut(s) 173, 258
BstYI RGATCY 2 cut(s) 173, 258
BsuRI GGCC 1 cut(s) 87
BtgI CCRYGG 1 cut(s) 88
BtgZI GCGATG 1 cut(s) 251
BtsCI GGATG 1 cut(s) 536
BtsI GCAGTG 1 cut(s) 555
BtsIMutI CAGTG 1 cut(s) 555
BveI ACCTGC 2 cut(s) 345, 404
Cac8I GCNNGC 1 cut(s) 369
CfoI GCGC 1 cut(s) 432
CseI GACGC 1 cut(s) 189
Csp6I GTAC 2 cut(s) 156, 507
CviAII CATG 1 cut(s) 362
CviQI GTAC 2 cut(s) 156, 507
DdeI CTNAG 3 cut(s) 372, 442, 510
DinI GGCGCC 1 cut(s) 431
DpnI GATC 5 cut(s) 104, 175, 260, 499, 581
DpnII GATC 5 cut(s) 102, 173, 258, 497, 579
EaeI YGGCCR 1 cut(s) 85
Ecl136II GAGCTC 1 cut(s) 441
Eco130I CCWWGG 1 cut(s) 297
Eco24I GRGCYC 3 cut(s) 328, 443, 467
Eco53kI GAGCTC 1 cut(s) 441
Eco57I CTGAAG 1 cut(s) 189
EcoICRI GAGCTC 1 cut(s) 441
EcoRII CCWGG 1 cut(s) 547
EcoT14I CCWWGG 1 cut(s) 297
EcoT38I GRGCYC 3 cut(s) 328, 443, 467
EgeI GGCGCC 1 cut(s) 431
EheI GGCGCC 1 cut(s) 431
ErhI CCWWGG 1 cut(s) 297
Esp3I CGTCTC 1 cut(s) 322
FaeI CATG 1 cut(s) 365
FaiI YATR 6 cut(s) 110, 276, 363, 456, 531, 533
FatI CATG 1 cut(s) 361
FauI CCCGC 1 cut(s) 89
FauNDI CATATG 1 cut(s) 531
FbaI TGATCA 1 cut(s) 102
Fnu4HI GCNGC 6 cut(s) 147, 271, 303, 352, 433, 472
FokI GGATG 1 cut(s) 523
FriOI GRGCYC 3 cut(s) 328, 443, 467
Fsp4HI GCNGC 6 cut(s) 147, 271, 303, 352, 433, 472
FspBI CTAG 1 cut(s) 368
GlaI GCGC 1 cut(s) 431
GluI GCNGC 6 cut(s) 147, 271, 303, 352, 433, 472
GsaI CCCAGC 2 cut(s) 153, 545
HaeII RGCGCY 1 cut(s) 433
HaeIII GGCC 1 cut(s) 87
HapII CCGG 2 cut(s) 321, 475
HgaI GACGC 1 cut(s) 189
HhaI GCGC 1 cut(s) 432
Hin1I GRCGYC 1 cut(s) 430
Hin1II CATG 1 cut(s) 365
Hin6I GCGC 1 cut(s) 430
HinP1I GCGC 1 cut(s) 430
HinfI GANTC 1 cut(s) 62
HpaII CCGG 2 cut(s) 321, 475
HphI GGTGA 2 cut(s) 260, 458
Hpy166II GTNNAC 1 cut(s) 449
Hpy188I TCNGA 2 cut(s) 67, 502
Hpy188III TCNNGA 2 cut(s) 171, 577
Hpy8I GTNNAC 1 cut(s) 449
Hpy99I CGWCG 1 cut(s) 408
HpyCH4III ACNGT 1 cut(s) 92
HpyCH4IV ACGT 1 cut(s) 406
HpyCH4V TGCA 2 cut(s) 254, 351
HpyF10VI GCNNNNNNNGC 4 cut(s) 93, 206, 438, 471
HpyF3I CTNAG 3 cut(s) 372, 442, 510
HpySE526I ACGT 1 cut(s) 406
Hsp92I GRCGYC 1 cut(s) 430
Hsp92II CATG 1 cut(s) 365
HspAI GCGC 1 cut(s) 430
KasI GGCGCC 1 cut(s) 429
Ksp22I TGATCA 1 cut(s) 102
Kzo9I GATC 5 cut(s) 102, 173, 258, 497, 579
LmnI GCTCC 1 cut(s) 197
Lsp1109I GCAGC 3 cut(s) 158, 314, 363
LweI GCATC 2 cut(s) 18, 241
MaeI CTAG 1 cut(s) 368
MaeII ACGT 1 cut(s) 406
MaeIII GTNAC 1 cut(s) 416
MalI GATC 5 cut(s) 104, 175, 260, 499, 581
MboI GATC 5 cut(s) 102, 173, 258, 497, 579
MboII GAAGA 4 cut(s) 68, 83, 131, 195
MflI RGATCY 2 cut(s) 173, 258
MhlI GDGCHC 3 cut(s) 328, 443, 467
MlsI TGGCCA 1 cut(s) 87
MluCI AATT 1 cut(s) 562
MluNI TGGCCA 1 cut(s) 87
Mly113I GGCGCC 1 cut(s) 430
MlyI GAGTC 1 cut(s) 56
MnlI CCTC 5 cut(s) 179, 185, 327, 420, 454
Mox20I TGGCCA 1 cut(s) 87
MscI TGGCCA 1 cut(s) 87
Msp20I TGGCCA 1 cut(s) 87
MspA1I CMGCKG 1 cut(s) 149
MspI CCGG 2 cut(s) 321, 475
MspR9I CCNGG 3 cut(s) 322, 476, 549
Mva1269I GAATGC 1 cut(s) 46
MvaI CCWGG 1 cut(s) 549
MwoI GCNNNNNNNGC 4 cut(s) 93, 206, 438, 471
NarI GGCGCC 1 cut(s) 430
NciI CCSGG 2 cut(s) 322, 476
NdeI CATATG 1 cut(s) 531
NdeII GATC 5 cut(s) 102, 173, 258, 497, 579
NheI GCTAGC 1 cut(s) 367
NlaIII CATG 1 cut(s) 365
NlaIV GGNNCC 3 cut(s) 175, 359, 431
NmuCI GTSAC 1 cut(s) 416
PctI GAATGC 1 cut(s) 46
PkrI GCNGC 6 cut(s) 148, 272, 304, 353, 434, 473
PleI GAGTC 1 cut(s) 56
PluTI GGCGCC 1 cut(s) 433
PpsI GAGTC 1 cut(s) 56
Psp124BI GAGCTC 1 cut(s) 443
Psp6I CCWGG 1 cut(s) 547
PspFI CCCAGC 2 cut(s) 149, 541
PspGI CCWGG 1 cut(s) 547
PspN4I GGNNCC 3 cut(s) 175, 359, 431
PsuI RGATCY 2 cut(s) 173, 258
PvuII CAGCTG 1 cut(s) 149
RsaI GTAC 2 cut(s) 157, 508
RsaNI GTAC 2 cut(s) 156, 507
SacI GAGCTC 1 cut(s) 443
SatI GCNGC 6 cut(s) 147, 271, 303, 352, 433, 472
Sau3AI GATC 5 cut(s) 102, 173, 258, 497, 579
ScaI AGTACT 2 cut(s) 157, 508
SchI GAGTC 1 cut(s) 56
ScrFI CCNGG 3 cut(s) 322, 476, 549
SduI GDGCHC 3 cut(s) 328, 443, 467
SfaNI GCATC 2 cut(s) 18, 241
SfoI GGCGCC 1 cut(s) 431
SmlI CTYRAG 1 cut(s) 466
SmoI CTYRAG 1 cut(s) 466
Sse9I AATT 1 cut(s) 562
SsiI CCGC 5 cut(s) 96, 183, 271, 433, 472
SspDI GGCGCC 1 cut(s) 429
SspMI CTAG 1 cut(s) 368
SstI GAGCTC 1 cut(s) 443
StyD4I CCNGG 3 cut(s) 320, 474, 547
StyI CCWWGG 1 cut(s) 297
TaaI ACNGT 1 cut(s) 92
TaiI ACGT 1 cut(s) 409
TaqI TCGA 1 cut(s) 328
TasI AATT 1 cut(s) 562
TatI WGTACW 2 cut(s) 155, 506
TauI GCSGC 3 cut(s) 273, 435, 474
TscAI CASTG 1 cut(s) 562
TseFI GTSAC 1 cut(s) 416
TseI GCWGC 3 cut(s) 146, 302, 351
Tsp45I GTSAC 1 cut(s) 416
TspDTI ATGAA 2 cut(s) 63, 396
TspRI CASTG 1 cut(s) 562
XspI CTAG 1 cut(s) 368
ZrmI AGTACT 2 cut(s) 157, 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.