MD03G1262500.v1.1

Transcriptional repressor, ovate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003569
Physical Location & Seq
Forward (+)
34847923 .. 34848669
747 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1262500.v1.1.491

Sequence Viewer

Length: 747 bp
ATGCAAAACACACTGGGAAGAAACCTAAATCTCTGCTTCACAAAGATCCGACGGCCACCAATGAGTACAACCCTAGATGACAACGATCACAGCCGTCCATTACCAACCTCCACCGCCACCGCCACCGCCACCGCATCCACATCAACGTCCATGATTAAAAACTTCAACTCCCTCTACGACGACAAATCCACGCTGTTCGACTCCACCACCACCACCTCAAAATCCCTCAGCTCTTCCCTCCTCTCCACCCCCGACGACTACTCCGACACCGATGCCCCTGCCCCCGACTTTGCCACCGCCTTTGCCTCCCGACGCTTCTTCTTCTCCTCTCCCGGCCTCTCCAACTCCATTGTCGACCAATCGTCATTTGCCGCCACCTCATCATCCTCCAAAACATCAACCTCCGAGACCGAAGACCGCAAGCTCTTCATCAACCACAGCGTGGCGGTCCCCACATTTTCCCCCGATCCATATCGGGACTTCCTGCGGTCCATGCAGGAGATGGTGGAGGCGCGTGAGAGAACGCCAGATTCGGAGGACGTGAAGAAGAAATCCAACTGGGAGTTTTTGCATGAGCTCCTCCTGTGCTATCTTGCGCTGAACCCTAAGAGCACTCACAAGTTTATTATCGGCGCTTTCGCCGATCTTCTCGTCAGCCTCATGCCATCTCCCTGCGGAGGCGCCCGCATTGAATTAGAACCGGAGTTCACCGCTGGCGTTTGCGAGGTTTCACGCCGCCCGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ovate

Protein Analysis

249

Amino Acids

27.34

Weight (kDa)

5.56

Isoelectric Point (pI)

49.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ovate PF04844 154 - 220 5.8e-24 Transcriptional repressor, ovate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 680
AccB7I CCANNNNNTGG 1 cut(s) 442
AccI GTMKAC 1 cut(s) 354
AccII CGCG 1 cut(s) 514
AclWI GGATC 2 cut(s) 40, 461
AcoI YGGCCR 1 cut(s) 53
AcyI GRCGYC 1 cut(s) 681
AdeI CACNNNGTG 1 cut(s) 442
AfaI GTAC 1 cut(s) 67
AfiI CCNNNNNNNGG 2 cut(s) 442, 677
AgsI TTSAA 2 cut(s) 166, 692
AhdI GACNNNNNGTC 1 cut(s) 361
AjiI CACGTC 1 cut(s) 541
AluBI AGCT 3 cut(s) 231, 424, 577
AluI AGCT 3 cut(s) 231, 424, 577
Alw21I GWGCWC 2 cut(s) 579, 614
Alw26I GTCTC 1 cut(s) 401
AlwI GGATC 2 cut(s) 40, 461
AoxI GGCC 2 cut(s) 53, 334
AspLEI GCGC 4 cut(s) 514, 598, 635, 683
AspS9I GGNCC 2 cut(s) 448, 489
AsuC2I CCSGG 1 cut(s) 333
AsuHPI GGTGA 1 cut(s) 700
AvaII GGWCC 2 cut(s) 448, 489
BanI GGYRCC 1 cut(s) 680
BanII GRGCYC 1 cut(s) 579
BbsI GAAGAC 1 cut(s) 420
Bbv12I GWGCWC 2 cut(s) 579, 614
BbvCI CCTCAGC 1 cut(s) 227
BccI CCATC 2 cut(s) 496, 673
BceAI ACGGC 2 cut(s) 68, 78
BcgI CGANNNNNNTGC 4 cut(s) 254, 260, 288, 294
BcnI CCSGG 1 cut(s) 333
BcoDI GTCTC 1 cut(s) 401
BfaI CTAG 1 cut(s) 74
BfoI RGCGCY 2 cut(s) 636, 684
BisI GCNGC 2 cut(s) 372, 736
BlsI GCNGC 2 cut(s) 373, 737
Bme1390I CCNGG 1 cut(s) 333
Bme18I GGWCC 2 cut(s) 448, 489
BmeRI GACNNNNNGTC 1 cut(s) 361
BmgBI CACGTC 1 cut(s) 541
BmgT120I GGNCC 2 cut(s) 448, 489
BmiI GGNNCC 2 cut(s) 450, 682
BmrFI CCNGG 1 cut(s) 333
BmrI ACTGGG 2 cut(s) 23, 568
BmsI GCATC 2 cut(s) 143, 262
BmuI ACTGGG 2 cut(s) 23, 568
BpiI GAAGAC 1 cut(s) 420
Bpu10I CCTNAGC 1 cut(s) 227
BpuMI CCSGG 1 cut(s) 333
BsaBI GATNNNNATC 1 cut(s) 471
BsaHI GRCGYC 1 cut(s) 681
BsaI GGTCTC 1 cut(s) 401
BsaWI WCCGGW 1 cut(s) 700
BsaXI ACNNNNNCTCC 4 cut(s) 152, 182, 245, 275
Bsc4I CCNNNNNNNGG 2 cut(s) 442, 677
Bse1I ACTGG 2 cut(s) 18, 563
Bse8I GATNNNNATC 1 cut(s) 471
BseGI GGATG 2 cut(s) 134, 383
BseJI GATNNNNATC 1 cut(s) 471
BseLI CCNNNNNNNGG 2 cut(s) 442, 677
BseMII CTCAG 1 cut(s) 241
BseNI ACTGG 2 cut(s) 18, 563
BseRI GAGGAG 3 cut(s) 230, 316, 569
Bsh1236I CGCG 1 cut(s) 514
BshFI GGCC 2 cut(s) 55, 336
BshNI GGYRCC 1 cut(s) 680
BsiHKAI GWGCWC 2 cut(s) 579, 614
BsiSI CCGG 2 cut(s) 333, 701
BslFI GGGAC 2 cut(s) 434, 491
BslI CCNNNNNNNGG 2 cut(s) 442, 677
BsmAI GTCTC 1 cut(s) 401
BsmFI GGGAC 2 cut(s) 434, 491
BsnI GGCC 2 cut(s) 55, 336
Bso31I GGTCTC 1 cut(s) 401
Bsp1286I GDGCHC 2 cut(s) 579, 614
Bsp143I GATC 4 cut(s) 45, 85, 466, 643
BspANI GGCC 2 cut(s) 55, 336
BspCNI CTCAG 1 cut(s) 240
BspFNI CGCG 1 cut(s) 514
BspLI GGNNCC 2 cut(s) 450, 682
BspPI GGATC 2 cut(s) 40, 461
BspQI GCTCTTC 2 cut(s) 238, 431
BspT107I GGYRCC 1 cut(s) 680
BspTNI GGTCTC 1 cut(s) 401
BsrI ACTGG 2 cut(s) 18, 563
BssMI GATC 4 cut(s) 45, 85, 466, 643
BssNI GRCGYC 1 cut(s) 681
Bst6I CTCTTC 2 cut(s) 238, 431
BstACI GRCGYC 1 cut(s) 681
BstC8I GCNNGC 4 cut(s) 422, 685, 715, 740
BstDEI CTNAG 2 cut(s) 227, 606
BstF5I GGATG 2 cut(s) 134, 383
BstFNI CGCG 1 cut(s) 514
BstH2I RGCGCY 2 cut(s) 636, 684
BstHHI GCGC 4 cut(s) 514, 598, 635, 683
BstKTI GATC 4 cut(s) 48, 88, 469, 646
BstMAI GTCTC 1 cut(s) 401
BstMBI GATC 4 cut(s) 45, 85, 466, 643
BstMWI GCNNNNNNNGC 1 cut(s) 493
BstSCI CCNGG 1 cut(s) 331
BstUI CGCG 1 cut(s) 514
BstV2I GAAGAC 1 cut(s) 420
BstX2I RGATCY 1 cut(s) 45
BstYI RGATCY 1 cut(s) 45
BsuRI GGCC 2 cut(s) 55, 336
BtrI CACGTC 1 cut(s) 541
BtsCI GGATG 2 cut(s) 134, 383
BtsIMutI CAGTG 1 cut(s) 11
Cac8I GCNNGC 4 cut(s) 422, 685, 715, 740
CfoI GCGC 4 cut(s) 514, 598, 635, 683
Cfr13I GGNCC 2 cut(s) 448, 489
CseI GACGC 1 cut(s) 321
Csp6I GTAC 1 cut(s) 66
CspCI CAANNNNNGTGG 2 cut(s) 283, 318
CviAII CATG 4 cut(s) 151, 493, 572, 661
CviJI RGCY 7 cut(s) 55, 93, 231, 336, 424, 577, 657
CviKI_1 RGCY 7 cut(s) 55, 93, 231, 336, 424, 577, 657
CviQI GTAC 1 cut(s) 66
DdeI CTNAG 2 cut(s) 227, 606
DinI GGCGCC 1 cut(s) 682
DpnI GATC 4 cut(s) 47, 87, 468, 645
DpnII GATC 4 cut(s) 45, 85, 466, 643
DraIII CACNNNGTG 1 cut(s) 442
DriI GACNNNNNGTC 1 cut(s) 361
EaeI YGGCCR 1 cut(s) 53
Eam1104I CTCTTC 2 cut(s) 238, 431
Eam1105I GACNNNNNGTC 1 cut(s) 361
EarI CTCTTC 2 cut(s) 238, 431
Ecl136II GAGCTC 1 cut(s) 577
Eco24I GRGCYC 1 cut(s) 579
Eco31I GGTCTC 1 cut(s) 401
Eco47I GGWCC 2 cut(s) 448, 489
Eco53kI GAGCTC 1 cut(s) 577
EcoICRI GAGCTC 1 cut(s) 577
EcoT38I GRGCYC 1 cut(s) 579
EgeI GGCGCC 1 cut(s) 682
EheI GGCGCC 1 cut(s) 682
FaeI CATG 4 cut(s) 154, 496, 575, 664
FaiI YATR 5 cut(s) 152, 472, 494, 573, 662
FaqI GGGAC 2 cut(s) 434, 491
FatI CATG 4 cut(s) 150, 492, 571, 660
FauI CCCGC 1 cut(s) 692
FblI GTMKAC 1 cut(s) 354
Fnu4HI GCNGC 2 cut(s) 372, 736
FokI GGATG 2 cut(s) 121, 370
FriOI GRGCYC 1 cut(s) 579
Fsp4HI GCNGC 2 cut(s) 372, 736
FspBI CTAG 1 cut(s) 74
GlaI GCGC 4 cut(s) 513, 597, 634, 682
GluI GCNGC 2 cut(s) 372, 736
HaeII RGCGCY 2 cut(s) 636, 684
HaeIII GGCC 2 cut(s) 55, 336
HapII CCGG 2 cut(s) 333, 701
HgaI GACGC 1 cut(s) 321
HhaI GCGC 4 cut(s) 514, 598, 635, 683
Hin1I GRCGYC 1 cut(s) 681
Hin1II CATG 4 cut(s) 154, 496, 575, 664
Hin6I GCGC 4 cut(s) 512, 596, 633, 681
HinP1I GCGC 4 cut(s) 512, 596, 633, 681
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HinfI GANTC 2 cut(s) 200, 530
HpaII CCGG 2 cut(s) 333, 701
HphI GGTGA 1 cut(s) 700
Hpy166II GTNNAC 2 cut(s) 355, 708
Hpy188I TCNGA 4 cut(s) 50, 265, 406, 535
Hpy188III TCNNGA 2 cut(s) 309, 476
Hpy8I GTNNAC 2 cut(s) 355, 708
Hpy99I CGWCG 4 cut(s) 54, 182, 257, 315
HpyCH4IV ACGT 2 cut(s) 146, 540
HpyCH4V TGCA 3 cut(s) 4, 496, 571
HpyF10VI GCNNNNNNNGC 1 cut(s) 493
HpyF3I CTNAG 2 cut(s) 227, 606
HpySE526I ACGT 2 cut(s) 146, 540
Hsp92I GRCGYC 1 cut(s) 681
Hsp92II CATG 4 cut(s) 154, 496, 575, 664
HspAI GCGC 4 cut(s) 512, 596, 633, 681
KasI GGCGCC 1 cut(s) 680
Kzo9I GATC 4 cut(s) 45, 85, 466, 643
LguI GCTCTTC 2 cut(s) 238, 431
LmnI GCTCC 1 cut(s) 582
LweI GCATC 2 cut(s) 143, 262
MaeI CTAG 1 cut(s) 74
MaeII ACGT 2 cut(s) 146, 540
MalI GATC 4 cut(s) 47, 87, 468, 645
MboI GATC 4 cut(s) 45, 85, 466, 643
MboII GAAGA 9 cut(s) 30, 225, 310, 313, 418, 425, 556, 559, 638
MflI RGATCY 1 cut(s) 45
MhlI GDGCHC 2 cut(s) 579, 614
MluCI AATT 1 cut(s) 692
Mly113I GGCGCC 1 cut(s) 681
MlyI GAGTC 1 cut(s) 194
MmeI TCCRAC 4 cut(s) 73, 288, 366, 579
MseI TTAA 1 cut(s) 156
MspA1I CMGCKG 1 cut(s) 713
MspI CCGG 2 cut(s) 333, 701
MspR9I CCNGG 1 cut(s) 333
MvnI CGCG 1 cut(s) 514
MwoI GCNNNNNNNGC 1 cut(s) 493
NarI GGCGCC 1 cut(s) 681
NciI CCSGG 1 cut(s) 333
NdeII GATC 4 cut(s) 45, 85, 466, 643
NlaIII CATG 4 cut(s) 154, 496, 575, 664
NlaIV GGNNCC 2 cut(s) 450, 682
PciSI GCTCTTC 2 cut(s) 238, 431
PcsI WCGNNNNNNNCGW 1 cut(s) 261
PfeI GAWTC 1 cut(s) 530
PflMI CCANNNNNTGG 1 cut(s) 442
PkrI GCNGC 2 cut(s) 373, 737
PleI GAGTC 1 cut(s) 194
PluTI GGCGCC 1 cut(s) 684
PpsI GAGTC 1 cut(s) 194
Psp124BI GAGCTC 1 cut(s) 579
PspN4I GGNNCC 2 cut(s) 450, 682
PspPI GGNCC 2 cut(s) 448, 489
PsuI RGATCY 1 cut(s) 45
RsaI GTAC 1 cut(s) 67
RsaNI GTAC 1 cut(s) 66
SacI GAGCTC 1 cut(s) 579
SalI GTCGAC 1 cut(s) 353
SapI GCTCTTC 2 cut(s) 238, 431
SaqAI TTAA 1 cut(s) 156
SatI GCNGC 2 cut(s) 372, 736
Sau3AI GATC 4 cut(s) 45, 85, 466, 643
Sau96I GGNCC 2 cut(s) 448, 489
SchI GAGTC 1 cut(s) 194
ScrFI CCNGG 1 cut(s) 333
SduI GDGCHC 2 cut(s) 579, 614
SfaNI GCATC 2 cut(s) 143, 262
SfoI GGCGCC 1 cut(s) 682
SinI GGWCC 2 cut(s) 448, 489
Sse9I AATT 1 cut(s) 692
SspDI GGCGCC 1 cut(s) 680
SspMI CTAG 1 cut(s) 74
SstI GAGCTC 1 cut(s) 579
StyD4I CCNGG 1 cut(s) 331
TaiI ACGT 2 cut(s) 149, 543
TaqI TCGA 2 cut(s) 198, 354
TaqII GACCGA 1 cut(s) 425
TasI AATT 1 cut(s) 692
TatI WGTACW 1 cut(s) 65
TauI GCSGC 2 cut(s) 374, 738
TfiI GAWTC 1 cut(s) 530
Tru1I TTAA 1 cut(s) 156
Tru9I TTAA 1 cut(s) 156
TscAI CASTG 1 cut(s) 18
TspDTI ATGAA 1 cut(s) 418
TspRI CASTG 1 cut(s) 18
Van91I CCANNNNNTGG 1 cut(s) 442
VpaK11BI GGWCC 2 cut(s) 448, 489
XcmI CCANNNNNNNNNTGG 1 cut(s) 499
XmiI GTMKAC 1 cut(s) 354
XspI CTAG 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.