pycom11g25070

Transcriptional repressor, ovate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0003569
Physical Location & Seq
Forward (+)
27955559 .. 27956305
747 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g25070.1

Sequence Viewer

Length: 747 bp
ATGGCGAACACATTGGGAAGAAACCTAAATCTCTGCTTCACAAAGATCCGACGGCCACCAATGGGTACAACCCTAGATGACGACGCTCACAGCCGTCCATTACCAACCGCCACCGCATCCACATCCACATCCACATCCACGTCACCAACGTCCATGATTAAAAACTTTAACTCCCTCTACGACGACCAGTCCACGTTGTTCGACTCCACCAACACCACCTCAAAATCCCTCAGCTCCTCCCTCCTCTCCACCTCCGACGACTACACGGACACAGACTCCCCCACCCCCGACTTTGCCACTGCCTTCGCCTCCCGACGCTTCTTCTTCTCCTCTCCCGGCCGATCCAACTCCATCGTTGACCAATCGTCATTCGCCGCCACGTCATCCTTGTCCAAAACATCAGCCTCCGAGCCCGAAGACCGCACGCTCTTCATCAACCACAGCGTGGCGATCCCCACATTCTCCCCGGATCCCTACCGCGACTTCCGTCGGTCCATGCAGGAGATGGTGGAGGCGCGTGAGAGAACGCCAGACTCGGAGGACGTGAAGAAGAAATCCAACTGGGAGTTTTTGCATGAGCTCCTCCTGTGCTATCTTGCACTGAACCCTAAGAGCACCCACAAGTTTATTATCGGCGCTTTCGCTGATCTTCTTGTCAGCCTCATGCCGTCTCCCGCCGGAGGCTCCCGCATTGAATTAGAACCGGAGTTCACGGCCGGCGTTTGTGAGATTTCACGGTGCATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ovate

Protein Analysis

249

Amino Acids

27.29

Weight (kDa)

5.26

Isoelectric Point (pI)

52.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ovate PF04844 155 - 221 3.9e-25 Transcriptional repressor, ovate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 445
AccII CGCG 2 cut(s) 480, 517
AciI CCGC 7 cut(s) 108, 114, 375, 421, 478, 675, 688
AclWI GGATC 5 cut(s) 40, 336, 445, 464, 477
AcoI YGGCCR 3 cut(s) 53, 337, 714
AdeI CACNNNGTG 1 cut(s) 445
AfaI GTAC 1 cut(s) 67
AfiI CCNNNNNNNGG 3 cut(s) 62, 445, 680
AgsI TTSAA 1 cut(s) 695
AhdI GACNNNNNGTC 2 cut(s) 187, 364
AjiI CACGTC 3 cut(s) 141, 381, 544
AluBI AGCT 2 cut(s) 234, 580
AluI AGCT 2 cut(s) 234, 580
Alw21I GWGCWC 2 cut(s) 582, 617
Alw26I GTCTC 1 cut(s) 675
AlwI GGATC 5 cut(s) 40, 336, 445, 464, 477
AoxI GGCC 3 cut(s) 53, 337, 714
AspLEI GCGC 2 cut(s) 517, 638
AspS9I GGNCC 1 cut(s) 492
AsuC2I CCSGG 2 cut(s) 336, 467
AsuHPI GGTGA 1 cut(s) 135
AvaII GGWCC 1 cut(s) 492
BamHI GGATCC 1 cut(s) 469
BanII GRGCYC 2 cut(s) 414, 582
BbsI GAAGAC 1 cut(s) 423
Bbv12I GWGCWC 2 cut(s) 582, 617
BbvCI CCTCAGC 1 cut(s) 230
BccI CCATC 2 cut(s) 359, 499
BceAI ACGGC 4 cut(s) 68, 78, 652, 729
BcnI CCSGG 2 cut(s) 336, 467
BcoDI GTCTC 1 cut(s) 675
BfaI CTAG 2 cut(s) 74, 745
BfoI RGCGCY 1 cut(s) 639
BisI GCNGC 1 cut(s) 375
BlsI GCNGC 1 cut(s) 376
Bme1390I CCNGG 2 cut(s) 336, 467
Bme18I GGWCC 1 cut(s) 492
BmeRI GACNNNNNGTC 2 cut(s) 187, 364
BmgBI CACGTC 3 cut(s) 141, 381, 544
BmgT120I GGNCC 1 cut(s) 492
BmiI GGNNCC 2 cut(s) 471, 685
BmrFI CCNGG 2 cut(s) 336, 467
BmrI ACTGGG 1 cut(s) 571
BmsI GCATC 1 cut(s) 125
BmuI ACTGGG 1 cut(s) 571
BoxI GACNNNNGTC 1 cut(s) 486
BpiI GAAGAC 1 cut(s) 423
Bpu10I CCTNAGC 1 cut(s) 230
BpuMI CCSGG 2 cut(s) 336, 467
BsaJI CCNNGG 1 cut(s) 465
BsaWI WCCGGW 1 cut(s) 703
BsaXI ACNNNNNCTCC 4 cut(s) 155, 185, 260, 290
Bsc4I CCNNNNNNNGG 3 cut(s) 62, 445, 680
Bse118I RCCGGY 1 cut(s) 716
Bse1I ACTGG 2 cut(s) 187, 566
BseDI CCNNGG 1 cut(s) 465
BseGI GGATG 5 cut(s) 116, 122, 128, 134, 383
BseLI CCNNNNNNNGG 3 cut(s) 62, 445, 680
BseMII CTCAG 1 cut(s) 244
BseNI ACTGG 2 cut(s) 187, 566
BseRI GAGGAG 4 cut(s) 226, 233, 319, 572
BseX3I CGGCCG 2 cut(s) 337, 714
Bsh1236I CGCG 2 cut(s) 480, 517
Bsh1285I CGRYCG 2 cut(s) 340, 717
BshFI GGCC 3 cut(s) 55, 339, 716
BsiEI CGRYCG 2 cut(s) 340, 717
BsiHKAI GWGCWC 2 cut(s) 582, 617
BsiSI CCGG 5 cut(s) 336, 467, 678, 704, 717
BslI CCNNNNNNNGG 3 cut(s) 62, 445, 680
BsmAI GTCTC 1 cut(s) 675
BsmBI CGTCTC 1 cut(s) 675
BsnI GGCC 3 cut(s) 55, 339, 716
Bsp1286I GDGCHC 3 cut(s) 414, 582, 617
Bsp143I GATC 5 cut(s) 45, 341, 450, 469, 646
BspACI CCGC 7 cut(s) 108, 114, 375, 421, 478, 675, 688
BspANI GGCC 3 cut(s) 55, 339, 716
BspCNI CTCAG 1 cut(s) 243
BspFNI CGCG 2 cut(s) 480, 517
BspLI GGNNCC 2 cut(s) 471, 685
BspPI GGATC 5 cut(s) 40, 336, 445, 464, 477
BspQI GCTCTTC 1 cut(s) 434
BsrFI RCCGGY 1 cut(s) 716
BsrI ACTGG 2 cut(s) 187, 566
BssAI RCCGGY 1 cut(s) 716
BssECI CCNNGG 1 cut(s) 465
BssMI GATC 5 cut(s) 45, 341, 450, 469, 646
Bst4CI ACNGT 1 cut(s) 738
Bst6I CTCTTC 1 cut(s) 434
BstC8I GCNNGC 2 cut(s) 425, 718
BstDEI CTNAG 2 cut(s) 230, 609
BstF5I GGATG 5 cut(s) 116, 122, 128, 134, 383
BstFNI CGCG 2 cut(s) 480, 517
BstH2I RGCGCY 1 cut(s) 639
BstHHI GCGC 2 cut(s) 517, 638
BstKTI GATC 5 cut(s) 48, 344, 453, 472, 649
BstMAI GTCTC 1 cut(s) 675
BstMBI GATC 5 cut(s) 45, 341, 450, 469, 646
BstMCI CGRYCG 2 cut(s) 340, 717
BstPAI GACNNNNGTC 1 cut(s) 486
BstSCI CCNGG 2 cut(s) 334, 465
BstUI CGCG 2 cut(s) 480, 517
BstV2I GAAGAC 1 cut(s) 423
BstX2I RGATCY 2 cut(s) 45, 469
BstYI RGATCY 2 cut(s) 45, 469
BstZI CGGCCG 2 cut(s) 337, 714
BsuRI GGCC 3 cut(s) 55, 339, 716
BtrI CACGTC 3 cut(s) 141, 381, 544
BtsCI GGATG 5 cut(s) 116, 122, 128, 134, 383
BtsI GCAGTG 1 cut(s) 297
BtsIMutI CAGTG 2 cut(s) 297, 599
Cac8I GCNNGC 2 cut(s) 425, 718
CfoI GCGC 2 cut(s) 517, 638
Cfr10I RCCGGY 1 cut(s) 716
Cfr13I GGNCC 1 cut(s) 492
CseI GACGC 2 cut(s) 92, 324
Csp6I GTAC 1 cut(s) 66
CviAII CATG 4 cut(s) 154, 496, 575, 664
CviQI GTAC 1 cut(s) 66
DdeI CTNAG 2 cut(s) 230, 609
DpnI GATC 5 cut(s) 47, 343, 452, 471, 648
DpnII GATC 5 cut(s) 45, 341, 450, 469, 646
DraIII CACNNNGTG 1 cut(s) 445
DriI GACNNNNNGTC 2 cut(s) 187, 364
EaeI YGGCCR 3 cut(s) 53, 337, 714
EagI CGGCCG 2 cut(s) 337, 714
Eam1104I CTCTTC 1 cut(s) 434
Eam1105I GACNNNNNGTC 2 cut(s) 187, 364
EarI CTCTTC 1 cut(s) 434
Ecl136II GAGCTC 1 cut(s) 580
EclXI CGGCCG 2 cut(s) 337, 714
Eco24I GRGCYC 2 cut(s) 414, 582
Eco47I GGWCC 1 cut(s) 492
Eco52I CGGCCG 2 cut(s) 337, 714
Eco53kI GAGCTC 1 cut(s) 580
EcoICRI GAGCTC 1 cut(s) 580
EcoT38I GRGCYC 2 cut(s) 414, 582
Esp3I CGTCTC 1 cut(s) 675
FaeI CATG 4 cut(s) 157, 499, 578, 667
FaiI YATR 4 cut(s) 155, 497, 576, 665
FatI CATG 4 cut(s) 153, 495, 574, 663
FauI CCCGC 2 cut(s) 682, 695
Fnu4HI GCNGC 1 cut(s) 375
FokI GGATG 5 cut(s) 103, 109, 115, 121, 370
FriOI GRGCYC 2 cut(s) 414, 582
Fsp4HI GCNGC 1 cut(s) 375
FspBI CTAG 2 cut(s) 74, 745
GlaI GCGC 2 cut(s) 516, 637
GluI GCNGC 1 cut(s) 375
HaeII RGCGCY 1 cut(s) 639
HaeIII GGCC 3 cut(s) 55, 339, 716
HapII CCGG 5 cut(s) 336, 467, 678, 704, 717
HgaI GACGC 2 cut(s) 92, 324
HhaI GCGC 2 cut(s) 517, 638
Hin1II CATG 4 cut(s) 157, 499, 578, 667
Hin6I GCGC 2 cut(s) 515, 636
HinP1I GCGC 2 cut(s) 515, 636
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HinfI GANTC 3 cut(s) 203, 275, 533
HpaII CCGG 5 cut(s) 336, 467, 678, 704, 717
HphI GGTGA 1 cut(s) 135
Hpy166II GTNNAC 3 cut(s) 192, 358, 711
Hpy188I TCNGA 4 cut(s) 50, 256, 409, 538
Hpy188III TCNNGA 1 cut(s) 312
Hpy8I GTNNAC 3 cut(s) 192, 358, 711
Hpy99I CGWCG 6 cut(s) 54, 86, 185, 260, 318, 492
HpyAV CCTTC 1 cut(s) 313
HpyCH4III ACNGT 1 cut(s) 738
HpyCH4IV ACGT 5 cut(s) 140, 149, 194, 380, 543
HpyCH4V TGCA 4 cut(s) 499, 574, 599, 741
HpyF3I CTNAG 2 cut(s) 230, 609
HpySE526I ACGT 5 cut(s) 140, 149, 194, 380, 543
Hsp92II CATG 4 cut(s) 157, 499, 578, 667
HspAI GCGC 2 cut(s) 515, 636
KroI GCCGGC 1 cut(s) 716
KroNI GCCGGC 1 cut(s) 718
Kzo9I GATC 5 cut(s) 45, 341, 450, 469, 646
LguI GCTCTTC 1 cut(s) 434
LmnI GCTCC 3 cut(s) 239, 585, 689
LweI GCATC 1 cut(s) 125
MaeI CTAG 2 cut(s) 74, 745
MaeII ACGT 5 cut(s) 140, 149, 194, 380, 543
MaeIII GTNAC 1 cut(s) 141
MalI GATC 5 cut(s) 47, 343, 452, 471, 648
MboI GATC 5 cut(s) 45, 341, 450, 469, 646
MboII GAAGA 8 cut(s) 30, 313, 316, 421, 428, 559, 562, 641
MflI RGATCY 2 cut(s) 45, 469
MhlI GDGCHC 3 cut(s) 414, 582, 617
MluCI AATT 1 cut(s) 695
MlyI GAGTC 3 cut(s) 197, 269, 527
MmeI TCCRAC 4 cut(s) 73, 279, 369, 582
MroNI GCCGGC 1 cut(s) 716
MseI TTAA 2 cut(s) 159, 168
MspI CCGG 5 cut(s) 336, 467, 678, 704, 717
MspR9I CCNGG 2 cut(s) 336, 467
MvnI CGCG 2 cut(s) 480, 517
NaeI GCCGGC 1 cut(s) 718
NciI CCSGG 2 cut(s) 336, 467
NdeII GATC 5 cut(s) 45, 341, 450, 469, 646
NgoMIV GCCGGC 1 cut(s) 716
NlaIII CATG 4 cut(s) 157, 499, 578, 667
NlaIV GGNNCC 2 cut(s) 471, 685
NmuCI GTSAC 1 cut(s) 141
PciSI GCTCTTC 1 cut(s) 434
PcsI WCGNNNNNNNCGW 1 cut(s) 146
PdiI GCCGGC 1 cut(s) 718
PflMI CCANNNNNTGG 1 cut(s) 445
PkrI GCNGC 1 cut(s) 376
PleI GAGTC 3 cut(s) 197, 269, 527
PpsI GAGTC 3 cut(s) 197, 269, 527
PshAI GACNNNNGTC 1 cut(s) 486
Psp124BI GAGCTC 1 cut(s) 582
PspN4I GGNNCC 2 cut(s) 471, 685
PspPI GGNCC 1 cut(s) 492
PsuI RGATCY 2 cut(s) 45, 469
RsaI GTAC 1 cut(s) 67
RsaNI GTAC 1 cut(s) 66
SacI GAGCTC 1 cut(s) 582
SapI GCTCTTC 1 cut(s) 434
SaqAI TTAA 2 cut(s) 159, 168
SatI GCNGC 1 cut(s) 375
Sau3AI GATC 5 cut(s) 45, 341, 450, 469, 646
Sau96I GGNCC 1 cut(s) 492
SchI GAGTC 3 cut(s) 197, 269, 527
ScrFI CCNGG 2 cut(s) 336, 467
SduI GDGCHC 3 cut(s) 414, 582, 617
SfaNI GCATC 1 cut(s) 125
SinI GGWCC 1 cut(s) 492
Sse9I AATT 1 cut(s) 695
SsiI CCGC 7 cut(s) 108, 114, 375, 421, 478, 675, 688
SspMI CTAG 2 cut(s) 74, 745
SstI GAGCTC 1 cut(s) 582
StyD4I CCNGG 2 cut(s) 334, 465
TaaI ACNGT 1 cut(s) 738
TaiI ACGT 5 cut(s) 143, 152, 197, 383, 546
TaqI TCGA 1 cut(s) 201
TaqII GACCGA 1 cut(s) 480
TasI AATT 1 cut(s) 695
TauI GCSGC 1 cut(s) 377
Tru1I TTAA 2 cut(s) 159, 168
Tru9I TTAA 2 cut(s) 159, 168
TscAI CASTG 2 cut(s) 304, 606
TseFI GTSAC 1 cut(s) 141
Tsp45I GTSAC 1 cut(s) 141
TspDTI ATGAA 1 cut(s) 421
TspGWI ACGGA 2 cut(s) 281, 476
TspRI CASTG 2 cut(s) 304, 606
Van91I CCANNNNNTGG 1 cut(s) 445
VpaK11BI GGWCC 1 cut(s) 492
XcmI CCANNNNNNNNNTGG 1 cut(s) 502
XspI CTAG 2 cut(s) 74, 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.