MD13G1193900.v1.1

Transcriptional repressor, ovate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003569
Physical Location & Seq
Reverse (-)
16789668 .. 16790417
750 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1193900.v1.1.491

Sequence Viewer

Length: 750 bp
ATGTCTAAAATCCTATGCAAGAACTTCCACTGCTTCCCTAATTTCATCAAATGCCTACCCACAATCCAAGCTCCACCGGATCAAGCCTCAAACGAATTAGACCACCAAAACCAGCCACCACGTCCCTCTACCACCACATGTACATCTCTCATCATCAAGAACTTCAACTCCGTCTACCATGACTTCAACTTGATGAACTCATCATCAGATCACTCAACCTTCAAATCCCTCACAGCCTCCTCCTCCTCCACCGACGATCACTTCTACCACTCCTCCGACGATTCAGACGCCGACCAGTACTCCCCTCCAGATTTCGCCACCATCTTCGCCTCTCAACGCTTCTTCTTCTCCTCCCCGGGACAATCCAACTCCATCGTCGAGTCTCCAGACACCACCACAAAACCCAAGTCTTCTCAGAAAAGAATGTTGGTCACCAAAGGCGTGCGCGTTCCCAAGTATTCCTCAAACCCTTACATGGATTTTCGGCGTTCGATGGAGGAAATGCTCGAAGCGAATCGAGATGATATATCGGCAGGTGATCATGTTCATGCTACAAGCGATGACATGGAGTATTTACATGAGCTTCTCTTGTGCTACCTTTCGCTCAATCCGAAACACGCGCACAAGGACATTATCAGTGCCTTCACCGACCTTGTCATCGGCCTACTGTGTGCTGAAGATTCACCAGCACCTGCACCAGCCTCAGCGGCTGATATGAACGTGAAAAAAAGCCGGAAACTCATTAGTTAG

Protein Analysis

250

Amino Acids

27.75

Weight (kDa)

5.84

Isoelectric Point (pI)

49.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ovate PF04844 154 - 222 9.5e-20 Transcriptional repressor, ovate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 524, 700
Acc36I ACCTGC 2 cut(s) 524, 700
AccI GTMKAC 1 cut(s) 174
AccII CGCG 2 cut(s) 447, 620
AciI CCGC 1 cut(s) 707
AclWI GGATC 1 cut(s) 87
AcuI CTGAAG 1 cut(s) 696
AcyI GRCGYC 1 cut(s) 288
AfaI GTAC 2 cut(s) 142, 299
AfiI CCNNNNNNNGG 1 cut(s) 475
AflIII ACRYGT 1 cut(s) 137
AgsI TTSAA 3 cut(s) 166, 187, 223
AjiI CACGTC 1 cut(s) 122
AjuI GAANNNNNNNTTGG 2 cut(s) 410, 442
AloI GAACNNNNNNTCC 2 cut(s) 152, 184
AluBI AGCT 2 cut(s) 71, 583
AluI AGCT 2 cut(s) 71, 583
Alw26I GTCTC 1 cut(s) 387
AlwI GGATC 1 cut(s) 87
AlwNI CAGNNNCTG 2 cut(s) 692, 710
Ama87I CYCGRG 1 cut(s) 355
AoxI GGCC 1 cut(s) 661
ArsI GACNNNNNNTTYG 2 cut(s) 392, 424
AspLEI GCGC 2 cut(s) 447, 622
AsuC2I CCSGG 2 cut(s) 356, 357
AsuHPI GGTGA 4 cut(s) 424, 548, 637, 675
AvaI CYCGRG 1 cut(s) 355
BbsI GAAGAC 1 cut(s) 402
BbvCI CCTCAGC 1 cut(s) 703
BccI CCATC 3 cut(s) 329, 380, 487
BclI TGATCA 1 cut(s) 538
BcnI CCSGG 2 cut(s) 356, 357
BcoDI GTCTC 1 cut(s) 387
BfuAI ACCTGC 2 cut(s) 524, 700
BglI GCCNNNNNGGC 1 cut(s) 707
BisI GCNGC 1 cut(s) 708
BlsI GCNGC 1 cut(s) 709
BmcAI AGTACT 1 cut(s) 299
Bme1390I CCNGG 2 cut(s) 356, 357
BmeT110I CYCGRG 1 cut(s) 355
BmgBI CACGTC 1 cut(s) 122
BmrFI CCNGG 2 cut(s) 356, 357
BpiI GAAGAC 1 cut(s) 402
BpmI CTGGAG 2 cut(s) 291, 369
Bpu10I CCTNAGC 1 cut(s) 703
BpuMI CCSGG 2 cut(s) 356, 357
BsaHI GRCGYC 1 cut(s) 288
BsaJI CCNNGG 2 cut(s) 354, 355
BsaWI WCCGGW 1 cut(s) 76
BsaXI ACNNNNNCTCC 6 cut(s) 152, 182, 257, 284, 287, 314
Bsc4I CCNNNNNNNGG 1 cut(s) 475
Bse1I ACTGG 1 cut(s) 295
BseDI CCNNGG 2 cut(s) 354, 355
BseLI CCNNNNNNNGG 1 cut(s) 475
BseMII CTCAG 2 cut(s) 428, 717
BseNI ACTGG 1 cut(s) 295
BseRI GAGGAG 5 cut(s) 229, 232, 235, 262, 340
BsgI GTGCAG 1 cut(s) 678
Bsh1236I CGCG 2 cut(s) 447, 620
BshFI GGCC 1 cut(s) 663
BsiHKCI CYCGRG 1 cut(s) 355
BsiSI CCGG 3 cut(s) 77, 356, 733
BslFI GGGAC 2 cut(s) 108, 372
BslI CCNNNNNNNGG 1 cut(s) 475
BsmAI GTCTC 1 cut(s) 387
BsmFI GGGAC 2 cut(s) 108, 372
BsnI GGCC 1 cut(s) 663
BsoBI CYCGRG 1 cut(s) 355
Bsp1407I TGTACA 1 cut(s) 140
Bsp143I GATC 4 cut(s) 79, 208, 256, 538
BspACI CCGC 1 cut(s) 707
BspANI GGCC 1 cut(s) 663
BspCNI CTCAG 2 cut(s) 427, 716
BspFNI CGCG 2 cut(s) 447, 620
BspMI ACCTGC 2 cut(s) 524, 700
BspPI GGATC 1 cut(s) 87
BsrGI TGTACA 1 cut(s) 140
BsrI ACTGG 1 cut(s) 295
BssECI CCNNGG 2 cut(s) 354, 355
BssMI GATC 4 cut(s) 79, 208, 256, 538
BssNI GRCGYC 1 cut(s) 288
Bst4CI ACNGT 1 cut(s) 669
BstACI GRCGYC 1 cut(s) 288
BstAUI TGTACA 1 cut(s) 140
BstC8I GCNNGC 1 cut(s) 443
BstDEI CTNAG 2 cut(s) 414, 703
BstEII GGTNACC 1 cut(s) 430
BstFNI CGCG 2 cut(s) 447, 620
BstHHI GCGC 2 cut(s) 447, 622
BstKTI GATC 4 cut(s) 82, 211, 259, 541
BstMAI GTCTC 1 cut(s) 387
BstMBI GATC 4 cut(s) 79, 208, 256, 538
BstMWI GCNNNNNNNGC 1 cut(s) 707
BstNSI RCATGY 1 cut(s) 141
BstPI GGTNACC 1 cut(s) 430
BstSCI CCNGG 2 cut(s) 354, 355
BstUI CGCG 2 cut(s) 447, 620
BstV2I GAAGAC 1 cut(s) 402
BsuRI GGCC 1 cut(s) 663
BtgZI GCGATG 1 cut(s) 573
BtrI CACGTC 1 cut(s) 122
BtsI GCAGTG 1 cut(s) 28
BtsIMutI CAGTG 2 cut(s) 28, 643
BveI ACCTGC 2 cut(s) 524, 700
Cac8I GCNNGC 1 cut(s) 443
CaiI CAGNNNCTG 2 cut(s) 692, 710
CfoI GCGC 2 cut(s) 447, 622
Cfr9I CCCGGG 1 cut(s) 355
CseI GACGC 1 cut(s) 296
Csp6I GTAC 2 cut(s) 141, 298
CviAII CATG 7 cut(s) 138, 179, 475, 542, 548, 565, 578
CviJI RGCY 9 cut(s) 71, 86, 115, 236, 583, 663, 701, 710, 732
CviKI_1 RGCY 9 cut(s) 71, 86, 115, 236, 583, 663, 701, 710, 732
CviQI GTAC 2 cut(s) 141, 298
DdeI CTNAG 2 cut(s) 414, 703
DpnI GATC 4 cut(s) 81, 210, 258, 540
DpnII GATC 4 cut(s) 79, 208, 256, 538
Eco57I CTGAAG 1 cut(s) 696
Eco88I CYCGRG 1 cut(s) 355
Eco91I GGTNACC 1 cut(s) 430
EcoO65I GGTNACC 1 cut(s) 430
FaeI CATG 7 cut(s) 141, 182, 478, 545, 551, 568, 581
FaqI GGGAC 2 cut(s) 108, 372
FatI CATG 7 cut(s) 137, 178, 474, 541, 547, 564, 577
FbaI TGATCA 1 cut(s) 538
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 1 cut(s) 708
Fsp4HI GCNGC 1 cut(s) 708
GlaI GCGC 2 cut(s) 446, 621
GluI GCNGC 1 cut(s) 708
GsuI CTGGAG 2 cut(s) 291, 369
HaeIII GGCC 1 cut(s) 663
HapII CCGG 3 cut(s) 77, 356, 733
HgaI GACGC 1 cut(s) 296
HhaI GCGC 2 cut(s) 447, 622
Hin1I GRCGYC 1 cut(s) 288
Hin1II CATG 7 cut(s) 141, 182, 478, 545, 551, 568, 581
Hin6I GCGC 2 cut(s) 445, 620
HinP1I GCGC 2 cut(s) 445, 620
HinfI GANTC 4 cut(s) 281, 380, 514, 680
HpaII CCGG 3 cut(s) 77, 356, 733
HphI GGTGA 4 cut(s) 424, 548, 637, 675
Hpy166II GTNNAC 1 cut(s) 175
Hpy188I TCNGA 5 cut(s) 208, 277, 286, 417, 612
Hpy188III TCNNGA 4 cut(s) 157, 308, 386, 518
Hpy8I GTNNAC 1 cut(s) 175
Hpy99I CGWCG 3 cut(s) 257, 281, 380
HpyAV CCTTC 2 cut(s) 229, 652
HpyCH4III ACNGT 1 cut(s) 669
HpyCH4IV ACGT 2 cut(s) 121, 720
HpyCH4V TGCA 2 cut(s) 18, 695
HpyF10VI GCNNNNNNNGC 1 cut(s) 707
HpyF3I CTNAG 2 cut(s) 414, 703
HpySE526I ACGT 2 cut(s) 121, 720
Hsp92I GRCGYC 1 cut(s) 288
Hsp92II CATG 7 cut(s) 141, 182, 478, 545, 551, 568, 581
HspAI GCGC 2 cut(s) 445, 620
Ksp22I TGATCA 1 cut(s) 538
Kzo9I GATC 4 cut(s) 79, 208, 256, 538
LmnI GCTCC 1 cut(s) 76
MaeII ACGT 2 cut(s) 121, 720
MaeIII GTNAC 1 cut(s) 430
MalI GATC 4 cut(s) 81, 210, 258, 540
MboI GATC 4 cut(s) 79, 208, 256, 538
MboII GAAGA 5 cut(s) 316, 334, 337, 402, 689
MluCI AATT 2 cut(s) 40, 95
MlyI GAGTC 1 cut(s) 389
MmeI TCCRAC 2 cut(s) 300, 390
MslI CAYNNNNRTG 1 cut(s) 546
MspA1I CMGCKG 1 cut(s) 707
MspI CCGG 3 cut(s) 77, 356, 733
MspR9I CCNGG 2 cut(s) 356, 357
MvnI CGCG 2 cut(s) 447, 620
MwoI GCNNNNNNNGC 1 cut(s) 707
NciI CCSGG 2 cut(s) 356, 357
NdeII GATC 4 cut(s) 79, 208, 256, 538
NlaIII CATG 7 cut(s) 141, 182, 478, 545, 551, 568, 581
NmuCI GTSAC 1 cut(s) 430
NspI RCATGY 1 cut(s) 141
PaqCI CACCTGC 2 cut(s) 524, 700
PciI ACATGT 1 cut(s) 137
PcsI WCGNNNNNNNCGW 1 cut(s) 608
PfeI GAWTC 3 cut(s) 281, 514, 680
PflFI GACNNNGTC 1 cut(s) 653
PkrI GCNGC 1 cut(s) 709
PleI GAGTC 1 cut(s) 388
PpsI GAGTC 1 cut(s) 388
PscI ACATGT 1 cut(s) 137
PspEI GGTNACC 1 cut(s) 430
PstNI CAGNNNCTG 2 cut(s) 692, 710
PsyI GACNNNGTC 1 cut(s) 653
RsaI GTAC 2 cut(s) 142, 299
RsaNI GTAC 2 cut(s) 141, 298
RseI CAYNNNNRTG 1 cut(s) 546
SatI GCNGC 1 cut(s) 708
Sau3AI GATC 4 cut(s) 79, 208, 256, 538
ScaI AGTACT 1 cut(s) 299
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 2 cut(s) 356, 357
SetI ASST 9 cut(s) 73, 124, 221, 538, 585, 600, 654, 694, 723
SmaI CCCGGG 1 cut(s) 357
SmiMI CAYNNNNRTG 1 cut(s) 546
Sse9I AATT 2 cut(s) 40, 95
SsiI CCGC 1 cut(s) 707
StyD4I CCNGG 2 cut(s) 354, 355
TaaI ACNGT 1 cut(s) 669
TaiI ACGT 2 cut(s) 124, 723
TaqI TCGA 4 cut(s) 378, 491, 507, 517
TasI AATT 2 cut(s) 40, 95
TatI WGTACW 2 cut(s) 140, 297
TauI GCSGC 1 cut(s) 710
TfiI GAWTC 3 cut(s) 281, 514, 680
TscAI CASTG 2 cut(s) 35, 643
TseFI GTSAC 1 cut(s) 430
Tsp45I GTSAC 1 cut(s) 430
TspDTI ATGAA 4 cut(s) 34, 209, 536, 731
TspGWI ACGGA 1 cut(s) 160
TspMI CCCGGG 1 cut(s) 355
TspRI CASTG 2 cut(s) 35, 643
Tth111I GACNNNGTC 1 cut(s) 653
XceI RCATGY 1 cut(s) 141
XmaI CCCGGG 1 cut(s) 355
XmiI GTMKAC 1 cut(s) 174
ZrmI AGTACT 1 cut(s) 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.