MD04G1114100.v1.1

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002824
Physical Location & Seq
Forward (+)
20017288 .. 20020407
3120 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1114100.v1.1.491

Sequence Viewer

Length: 1080 bp
ATGCCGCCGAAGCAGCAATCGAAGGCCGATTTGGCGAAGAAGCAGAAGATCGTAGAGGACAAGACCTTCGGGCTCAAGAACAAGAACAAGAGCAAGAATGTCCAGAAGTATGTTCAGACCCTCAAGCAATCTGTCCAGCCGAAACCCGATCCTTCCAAGACCGCCGCTAAGAAGAAGAAGGAGGAAGAGAAGGCCAAGGACAAGGAACTGAATGACTTATTTAAGGTTGCTATTAGTCAACCGAAAGTACCAGTTGGTGTTGATCCCAAGTCTATACTATGTGAGTTCTATAAAGCGGGGCAGTGTGCAAAGGGTTTCAAGTGCAAGTTCTCACATGATTTGAATGTTCAGAGGAAAGGAGAAAAGATTGACATTTACAGTGATAAGCGTGATAATGAAGAAACAATGGAGGATTGGGATCAAGAAACTTTGGAGAAGATGGTCGAGTCGAAGAAAAATGAGTACCAGCAGAACAAACCAACTGACATTATCTGTAAATACTTCTTAGAAGCAGTGGAGAAGAAACAATATGGTTGGTTTTGGGTCTGTCCCAATGGAGGCAAAAATTGCCACTACAGGCATGCTCTTCCTCCAGGATATGTTTTGAAGTCACAGATGAAGGCTCTGCTGGACGAGGAGAGTGAAAAAATACCCATTGAGGAGGAGATTGAAAATCAGCGTGTTAAATTGACAACTTCAACACCTATGACTCCTGAGCTATTTATGCAATGGAAGAAGAAAAAGATTGCAGAAAGAGATGCCAGCTTAGCTGCAAAAATGGCAGAGCGAGCTAAGAATGACCGTATGAGTGGCCGTGAACTATTTTTGTCAGATTCTAGCTTATTTGTGGATGATGCTGAGGCAGATGAGAAGTACCACAAGGAGGAAGAACCTGCAGTTGCTGAAAAGAAGGCCAATGAGAACTCTAATGGAGATGGACCGAGCAACTCAGCAACAGCTGGTGGTGATGCTGAAGTTACCAATGTTGATGATGACGATGAACTGGACATAGATGAGTTAAACGAGTTGGAAGCAAGCTTATCGAATGTAGCAATTCAAGATCCAGGCACTCATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DFRP_C

Protein Analysis

360

Amino Acids

40.73

Weight (kDa)

5.59

Isoelectric Point (pI)

41.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DFRP_C PF16543 216 - 295 6.9e-19 DRG Family Regulatory Proteins, Tma46
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 901
AciI CCGC 4 cut(s) 5, 162, 165, 296
AclWI GGATC 4 cut(s) 143, 257, 426, 1055
AcoI YGGCCR 1 cut(s) 811
AcuI CTGAAG 1 cut(s) 993
AfaI GTAC 3 cut(s) 249, 464, 875
AfiI CCNNNNNNNGG 2 cut(s) 557, 883
AgsI TTSAA 6 cut(s) 319, 343, 607, 671, 699, 1058
AjnI CCWGG 2 cut(s) 592, 1063
AjuI GAANNNNNNNTTGG 2 cut(s) 14, 46
AluBI AGCT 7 cut(s) 718, 765, 770, 791, 840, 959, 1038
AluI AGCT 7 cut(s) 718, 765, 770, 791, 840, 959, 1038
AlwI GGATC 4 cut(s) 143, 257, 426, 1055
AlwNI CAGNNNCTG 1 cut(s) 902
AoxI GGCC 4 cut(s) 24, 192, 811, 912
ApeKI GCWGC 2 cut(s) 13, 770
ArsI GACNNNNNNTTYG 2 cut(s) 50, 82
AspS9I GGNCC 1 cut(s) 938
AsuHPI GGTGA 1 cut(s) 977
AvaII GGWCC 1 cut(s) 938
BanII GRGCYC 1 cut(s) 75
BbvCI CCTCAGC 1 cut(s) 858
BbvI GCAGC 2 cut(s) 25, 757
BccI CCATC 2 cut(s) 433, 929
BceAI ACGGC 1 cut(s) 798
BcgI CGANNNNNNTGC 2 cut(s) 1023, 1057
BciT130I CCWGG 2 cut(s) 594, 1065
BfaI CTAG 1 cut(s) 837
BfmI CTRYAG 2 cut(s) 574, 894
BfuAI ACCTGC 1 cut(s) 901
BglI GCCNNNNNGGC 1 cut(s) 32
BisI GCNGC 4 cut(s) 5, 14, 165, 771
BlpI GCTNAGC 1 cut(s) 766
BlsI GCNGC 4 cut(s) 6, 15, 166, 772
Bme1390I CCNGG 2 cut(s) 594, 1065
Bme18I GGWCC 1 cut(s) 938
BmgT120I GGNCC 1 cut(s) 938
BmrFI CCNGG 2 cut(s) 594, 1065
BmsI GCATC 3 cut(s) 748, 844, 958
BpmI CTGGAG 1 cut(s) 576
Bpu10I CCTNAGC 2 cut(s) 714, 858
Bpu1102I GCTNAGC 1 cut(s) 766
BpuEI CTTGAG 2 cut(s) 59, 107
BsaBI GATNNNNATC 1 cut(s) 417
BsaJI CCNNGG 1 cut(s) 195
Bsc4I CCNNNNNNNGG 2 cut(s) 557, 883
Bse1I ACTGG 2 cut(s) 251, 1008
Bse3DI GCAATG 1 cut(s) 734
Bse8I GATNNNNATC 1 cut(s) 417
BseBI CCWGG 2 cut(s) 594, 1065
BseDI CCNNGG 1 cut(s) 195
BseGI GGATG 1 cut(s) 856
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 2 cut(s) 557, 883
BseMI GCAATG 1 cut(s) 734
BseMII CTCAG 3 cut(s) 705, 849, 963
BseNI ACTGG 2 cut(s) 251, 1008
BseRI GAGGAG 3 cut(s) 650, 674, 677
BseXI GCAGC 2 cut(s) 25, 757
BshFI GGCC 4 cut(s) 26, 194, 813, 914
BslFI GGGAC 1 cut(s) 534
BslI CCNNNNNNNGG 2 cut(s) 557, 883
BsmFI GGGAC 1 cut(s) 534
BsnI GGCC 4 cut(s) 26, 194, 813, 914
Bsp1286I GDGCHC 1 cut(s) 75
Bsp143I GATC 5 cut(s) 48, 148, 262, 418, 1060
Bsp1720I GCTNAGC 1 cut(s) 766
BspACI CCGC 4 cut(s) 5, 162, 165, 296
BspANI GGCC 4 cut(s) 26, 194, 813, 914
BspCNI CTCAG 3 cut(s) 706, 850, 962
BspMAI CTGCAG 1 cut(s) 898
BspMI ACCTGC 1 cut(s) 901
BspPI GGATC 4 cut(s) 143, 257, 426, 1055
BspQI GCTCTTC 1 cut(s) 591
BsrDI GCAATG 1 cut(s) 734
BsrI ACTGG 2 cut(s) 251, 1008
BssECI CCNNGG 1 cut(s) 195
BssMI GATC 5 cut(s) 48, 148, 262, 418, 1060
BssT1I CCWWGG 1 cut(s) 195
Bst2UI CCWGG 2 cut(s) 594, 1065
Bst4CI ACNGT 2 cut(s) 380, 803
Bst6I CTCTTC 2 cut(s) 180, 591
BstAPI GCANNNNNTGC 1 cut(s) 567
BstC8I GCNNGC 4 cut(s) 582, 763, 789, 1036
BstDEI CTNAG 8 cut(s) 168, 505, 714, 766, 792, 858, 949, 1077
BstF5I GGATG 1 cut(s) 856
BstKTI GATC 5 cut(s) 51, 151, 265, 421, 1063
BstMBI GATC 5 cut(s) 48, 148, 262, 418, 1060
BstMWI GCNNNNNNNGC 8 cut(s) 10, 13, 32, 567, 724, 767, 779, 788
BstNI CCWGG 2 cut(s) 594, 1065
BstNSI RCATGY 1 cut(s) 584
BstSCI CCNGG 2 cut(s) 592, 1063
BstSFI CTRYAG 2 cut(s) 574, 894
BstV1I GCAGC 2 cut(s) 25, 757
BstX2I RGATCY 1 cut(s) 1060
BstYI RGATCY 1 cut(s) 1060
BsuRI GGCC 4 cut(s) 26, 194, 813, 914
BtsCI GGATG 1 cut(s) 856
BtsI GCAGTG 2 cut(s) 308, 519
BtsIMutI CAGTG 3 cut(s) 308, 385, 519
BveI ACCTGC 1 cut(s) 901
Cac8I GCNNGC 4 cut(s) 582, 763, 789, 1036
CaiI CAGNNNCTG 1 cut(s) 902
Cfr13I GGNCC 1 cut(s) 938
Csp6I GTAC 3 cut(s) 248, 463, 874
CviAII CATG 3 cut(s) 335, 581, 1073
CviQI GTAC 3 cut(s) 248, 463, 874
DdeI CTNAG 8 cut(s) 168, 505, 714, 766, 792, 858, 949, 1077
DpnI GATC 5 cut(s) 50, 150, 264, 420, 1062
DpnII GATC 5 cut(s) 48, 148, 262, 418, 1060
EaeI YGGCCR 1 cut(s) 811
Eam1104I CTCTTC 2 cut(s) 180, 591
EarI CTCTTC 2 cut(s) 180, 591
Eco130I CCWWGG 1 cut(s) 195
Eco24I GRGCYC 1 cut(s) 75
Eco47I GGWCC 1 cut(s) 938
Eco57I CTGAAG 1 cut(s) 993
EcoRII CCWGG 2 cut(s) 592, 1063
EcoT14I CCWWGG 1 cut(s) 195
EcoT38I GRGCYC 1 cut(s) 75
ErhI CCWWGG 1 cut(s) 195
FaeI CATG 3 cut(s) 338, 584, 1076
FaqI GGGAC 1 cut(s) 534
FatI CATG 3 cut(s) 334, 580, 1072
FauI CCCGC 1 cut(s) 289
Fnu4HI GCNGC 4 cut(s) 5, 14, 165, 771
FokI GGATG 1 cut(s) 863
FriOI GRGCYC 1 cut(s) 75
Fsp4HI GCNGC 4 cut(s) 5, 14, 165, 771
FspBI CTAG 1 cut(s) 837
GluI GCNGC 4 cut(s) 5, 14, 165, 771
GsuI CTGGAG 1 cut(s) 576
HaeIII GGCC 4 cut(s) 26, 194, 813, 914
Hin1II CATG 3 cut(s) 338, 584, 1076
HincII GTYRAC 1 cut(s) 239
HindII GTYRAC 1 cut(s) 239
HindIII AAGCTT 1 cut(s) 1036
HinfI GANTC 3 cut(s) 446, 709, 833
HphI GGTGA 1 cut(s) 977
Hpy166II GTNNAC 2 cut(s) 239, 818
Hpy188I TCNGA 3 cut(s) 117, 351, 832
Hpy188III TCNNGA 5 cut(s) 76, 103, 422, 713, 1058
Hpy8I GTNNAC 2 cut(s) 239, 818
HpyAV CCTTC 7 cut(s) 16, 76, 162, 172, 184, 613, 904
HpyCH4III ACNGT 2 cut(s) 380, 803
HpyCH4V TGCA 6 cut(s) 308, 324, 727, 749, 773, 896
HpyF10VI GCNNNNNNNGC 8 cut(s) 10, 13, 32, 567, 724, 767, 779, 788
HpyF3I CTNAG 8 cut(s) 168, 505, 714, 766, 792, 858, 949, 1077
Hsp92II CATG 3 cut(s) 338, 584, 1076
Kzo9I GATC 5 cut(s) 48, 148, 262, 418, 1060
LguI GCTCTTC 1 cut(s) 591
Lsp1109I GCAGC 2 cut(s) 25, 757
LweI GCATC 3 cut(s) 748, 844, 958
MaeI CTAG 1 cut(s) 837
MaeIII GTNAC 2 cut(s) 609, 976
MalI GATC 5 cut(s) 50, 150, 264, 420, 1062
MboI GATC 5 cut(s) 48, 148, 262, 418, 1060
MflI RGATCY 1 cut(s) 1060
MhlI GDGCHC 1 cut(s) 75
MluCI AATT 3 cut(s) 565, 686, 1053
MlyI GAGTC 2 cut(s) 455, 703
MmeI TCCRAC 1 cut(s) 1008
MseI TTAA 3 cut(s) 222, 684, 1019
MspA1I CMGCKG 1 cut(s) 959
MspR9I CCNGG 2 cut(s) 594, 1065
MvaI CCWGG 2 cut(s) 594, 1065
MwoI GCNNNNNNNGC 8 cut(s) 10, 13, 32, 567, 724, 767, 779, 788
NdeII GATC 5 cut(s) 48, 148, 262, 418, 1060
NlaIII CATG 3 cut(s) 338, 584, 1076
NmuCI GTSAC 1 cut(s) 609
NspI RCATGY 1 cut(s) 584
PaeI GCATGC 1 cut(s) 584
PciSI GCTCTTC 1 cut(s) 591
PfeI GAWTC 1 cut(s) 833
PfoI TCCNGGA 1 cut(s) 592
PkrI GCNGC 4 cut(s) 6, 15, 166, 772
PleI GAGTC 2 cut(s) 454, 703
PpsI GAGTC 2 cut(s) 454, 703
Psp6I CCWGG 2 cut(s) 592, 1063
PspGI CCWGG 2 cut(s) 592, 1063
PspPI GGNCC 1 cut(s) 938
PstI CTGCAG 1 cut(s) 898
PstNI CAGNNNCTG 1 cut(s) 902
PsuI RGATCY 1 cut(s) 1060
PvuII CAGCTG 1 cut(s) 959
RsaI GTAC 3 cut(s) 249, 464, 875
RsaNI GTAC 3 cut(s) 248, 463, 874
SapI GCTCTTC 1 cut(s) 591
SaqAI TTAA 3 cut(s) 222, 684, 1019
SatI GCNGC 4 cut(s) 5, 14, 165, 771
Sau3AI GATC 5 cut(s) 48, 148, 262, 418, 1060
Sau96I GGNCC 1 cut(s) 938
SchI GAGTC 2 cut(s) 455, 703
ScrFI CCNGG 2 cut(s) 594, 1065
SduI GDGCHC 1 cut(s) 75
SfaNI GCATC 3 cut(s) 748, 844, 958
SfcI CTRYAG 2 cut(s) 574, 894
SinI GGWCC 1 cut(s) 938
SmlI CTYRAG 2 cut(s) 74, 122
SmoI CTYRAG 2 cut(s) 74, 122
SphI GCATGC 1 cut(s) 584
Sse9I AATT 3 cut(s) 565, 686, 1053
SsiI CCGC 4 cut(s) 5, 162, 165, 296
SspMI CTAG 1 cut(s) 837
StyD4I CCNGG 2 cut(s) 592, 1063
StyI CCWWGG 1 cut(s) 195
TaaI ACNGT 2 cut(s) 380, 803
TaqI TCGA 4 cut(s) 20, 444, 449, 1043
TaqII GACCGA 1 cut(s) 955
TasI AATT 3 cut(s) 565, 686, 1053
TauI GCSGC 2 cut(s) 7, 167
TfiI GAWTC 1 cut(s) 833
Tru1I TTAA 3 cut(s) 222, 684, 1019
Tru9I TTAA 3 cut(s) 222, 684, 1019
TscAI CASTG 3 cut(s) 308, 385, 519
TseFI GTSAC 1 cut(s) 609
TseI GCWGC 2 cut(s) 13, 770
Tsp45I GTSAC 1 cut(s) 609
TspDTI ATGAA 3 cut(s) 411, 632, 1014
TspRI CASTG 3 cut(s) 308, 385, 519
VpaK11BI GGWCC 1 cut(s) 938
XceI RCATGY 1 cut(s) 584
XspI CTAG 1 cut(s) 837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.