MD04G1124800.v1.1

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000524
Physical Location & Seq
Reverse (-)
21160708 .. 21161019
312 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1124800.v1.1.491

Sequence Viewer

Length: 312 bp
ATGACTGGACGCGGCAAGGGAGGCAAGGGGTTGGGCAAAGGAGGAGCCAAGAGGCACAGAAAGGTGCTGAGGGACAACATCCAGGGTATCACCAAGCCCGCCATCCGCCGTCTGGCTCGCCGTGGCGGCGTCAAGCGTATCAGCGGCCTCATCTACGAGGAGACCCGCGGAGTGCTCAAGATCTTCTTGGAGAATGTGATCCGGGATGCTGTGACTTACACCGAGCATGCCCGGAGGAAGACTGTGACGGCGATGGACGTCGTCTATGCGCTCAAGAGGCAGGGTAGGACTCTGTACGGGTTCGGGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05203 ko05322 map05034 map05203 map05322
Pfam Domains
Protein Families
CENP-T_C

Protein Analysis

104

Amino Acids

11.42

Weight (kDa)

11.48

Isoelectric Point (pI)

39.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CENP-T_C PF15511 44 - 96 3.4e-08 Centromere kinetochore component CENP-T histone fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000524)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07660 AT1G07820 AT1G07820 AT2G28740 AT3G45930 AT3G46320 AT3G53730 AT5G59690 AT5G59970 AT5G59970
malus_domestica MD03G1097000.v1.1 MD03G1105400.v1.1 MD04G1124800.v1.1 MD06G1122500.v1.1 MD07G1184400.v1.1 MD07G1194500.v1.1 MD11G1143500.v1.1 MD12G1139800.v1.1 MD14G1139400.v1.1 MD15G1139200.v1.1
rosa_chinensis RchiOBHm_Chr1g0364991 RchiOBHm_Chr3g0467051 RchiOBHm_Chr5g0062221 RchiOBHm_Chr5g0063251 RchiOBHm_Chr5g0063281 RchiOBHm_Chr5g0063731 RchiOBHm_Chr7g0187711
rosa_laevigata RLG00000024533
rosa_multiflora Rmu_sc0004483.1_g000025 Rmu_sc0005045.1_g000017 Rmu_sc0005045.1_g000024 Rmu_sc0005638.1_g000003 Rmu_sc0042820.1_g000002 Rmu_sc0042820.1_g000003 Rmu_ssc0000112.1_g000021 Rmu_ssc0000309.1_g000047 Rmu_ssc0000409.1_g000022
rosa_roxburghii Rroxscaffold_1G00017140 Rroxscaffold_1G00017150 Rroxscaffold_1G00017430 Rroxscaffold_1G00017460 Rroxscaffold_4G00292020 Rroxscaffold_6G00413990
rosa_rugosa Rorug01G0318800 Rorug01G0329200 Rorug03G0085000 Rorug05G0347300 Rorug05G0355600 Rorug05G0355800 Rorug05G0358800 Rorug05G0358800 Rorug05G0358900 Rorug06G0483800
rosa_samantha Rh1AG327700 Rh1BG289000 Rh1CG305700 Rh1DG320500 Rh3AG133000 Rh3BG154000 Rh3CG154000 Rh3DG155100 Rh5AG407800 Rh5AG417800 Rh5BG421500 Rh5BG430300 Rh5BG433000 Rh5BG433200 Rh5CG445700 Rh5CG456200 Rh5CG456400 Rh5DG436500 Rh5DG443700 Rh5DG443900 Rh5DG446400 Rh5DG446600 Rh7BG090600 Rh7CG089600 Rh7DG091100
rosa_wichuraiana Rw1G029050 Rw3G012590 Rw5G038430 Rw5G039030 Rw5G039230 Rw5G039250 Rw7G007670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 261
AccII CGCG 2 cut(s) 12, 168
AciI CCGC 7 cut(s) 12, 99, 106, 126, 144, 166, 168
AclWI GGATC 1 cut(s) 193
AcyI GRCGYC 2 cut(s) 129, 258
AfaI GTAC 1 cut(s) 296
AfiI CCNNNNNNNGG 1 cut(s) 112
AjnI CCWGG 1 cut(s) 81
Alw21I GWGCWC 1 cut(s) 177
Alw26I GTCTC 1 cut(s) 155
AlwI GGATC 1 cut(s) 193
AoxI GGCC 1 cut(s) 145
AspLEI GCGC 1 cut(s) 271
AsuC2I CCSGG 2 cut(s) 203, 232
AsuHPI GGTGA 1 cut(s) 82
BbsI GAAGAC 1 cut(s) 245
Bbv12I GWGCWC 1 cut(s) 177
BbvCI CCTCAGC 1 cut(s) 68
BccI CCATC 2 cut(s) 110, 247
BceAI ACGGC 3 cut(s) 93, 105, 264
BciT130I CCWGG 1 cut(s) 83
BcnI CCSGG 2 cut(s) 203, 232
BcoDI GTCTC 1 cut(s) 155
BglI GCCNNNNNGGC 1 cut(s) 126
BglII AGATCT 1 cut(s) 180
BisI GCNGC 3 cut(s) 13, 127, 145
BlsI GCNGC 3 cut(s) 14, 128, 146
Bme1390I CCNGG 3 cut(s) 83, 203, 232
BmiI GGNNCC 1 cut(s) 46
BmrFI CCNGG 3 cut(s) 83, 203, 232
BmsI GCATC 1 cut(s) 196
BpiI GAAGAC 1 cut(s) 245
Bpu10I CCTNAGC 1 cut(s) 68
BpuEI CTTGAG 2 cut(s) 161, 257
BpuMI CCSGG 2 cut(s) 203, 232
BsaHI GRCGYC 2 cut(s) 129, 258
BsaI GGTCTC 1 cut(s) 155
BsaJI CCNNGG 3 cut(s) 82, 121, 166
Bsc4I CCNNNNNNNGG 1 cut(s) 112
Bse1I ACTGG 1 cut(s) 10
BseBI CCWGG 1 cut(s) 83
BseDI CCNNGG 3 cut(s) 82, 121, 166
BseGI GGATG 3 cut(s) 78, 102, 211
BseLI CCNNNNNNNGG 1 cut(s) 112
BseMII CTCAG 1 cut(s) 59
BseNI ACTGG 1 cut(s) 10
BseRI GAGGAG 2 cut(s) 57, 173
Bsh1236I CGCG 2 cut(s) 12, 168
BshFI GGCC 1 cut(s) 147
BsiHKAI GWGCWC 1 cut(s) 177
BsiSI CCGG 2 cut(s) 202, 232
BslFI GGGAC 1 cut(s) 86
BslI CCNNNNNNNGG 1 cut(s) 112
BsmAI GTCTC 1 cut(s) 155
BsmFI GGGAC 1 cut(s) 86
BsnI GGCC 1 cut(s) 147
Bso31I GGTCTC 1 cut(s) 155
Bsp1286I GDGCHC 1 cut(s) 177
Bsp143I GATC 2 cut(s) 180, 198
BspACI CCGC 7 cut(s) 12, 99, 106, 126, 144, 166, 168
BspANI GGCC 1 cut(s) 147
BspCNI CTCAG 1 cut(s) 60
BspFNI CGCG 2 cut(s) 12, 168
BspLI GGNNCC 1 cut(s) 46
BspPI GGATC 1 cut(s) 193
BspTNI GGTCTC 1 cut(s) 155
BsrI ACTGG 1 cut(s) 10
BssECI CCNNGG 3 cut(s) 82, 121, 166
BssMI GATC 2 cut(s) 180, 198
BssNI GRCGYC 2 cut(s) 129, 258
Bst2UI CCWGG 1 cut(s) 83
Bst4CI ACNGT 1 cut(s) 244
BstACI GRCGYC 2 cut(s) 129, 258
BstC8I GCNNGC 3 cut(s) 99, 118, 228
BstDEI CTNAG 1 cut(s) 68
BstDSI CCRYGG 2 cut(s) 121, 166
BstF5I GGATG 3 cut(s) 78, 102, 211
BstFNI CGCG 2 cut(s) 12, 168
BstHHI GCGC 1 cut(s) 271
BstKTI GATC 2 cut(s) 183, 201
BstMAI GTCTC 1 cut(s) 155
BstMBI GATC 2 cut(s) 180, 198
BstMWI GCNNNNNNNGC 3 cut(s) 21, 126, 277
BstNI CCWGG 1 cut(s) 83
BstNSI RCATGY 1 cut(s) 230
BstSCI CCNGG 3 cut(s) 81, 201, 230
BstUI CGCG 2 cut(s) 12, 168
BstV2I GAAGAC 1 cut(s) 245
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BsuRI GGCC 1 cut(s) 147
BtgI CCRYGG 2 cut(s) 121, 166
BtgZI GCGATG 1 cut(s) 266
BtsCI GGATG 3 cut(s) 78, 102, 211
Cac8I GCNNGC 3 cut(s) 99, 118, 228
CfoI GCGC 1 cut(s) 271
Cfr42I CCGCGG 1 cut(s) 169
CseI GACGC 2 cut(s) 18, 118
Csp6I GTAC 1 cut(s) 295
CviAII CATG 1 cut(s) 227
CviJI RGCY 4 cut(s) 47, 97, 116, 147
CviKI_1 RGCY 4 cut(s) 47, 97, 116, 147
CviQI GTAC 1 cut(s) 295
DdeI CTNAG 1 cut(s) 68
DpnI GATC 2 cut(s) 182, 200
DpnII GATC 2 cut(s) 180, 198
EciI GGCGGA 1 cut(s) 95
Eco31I GGTCTC 1 cut(s) 155
EcoRII CCWGG 1 cut(s) 81
FaeI CATG 1 cut(s) 230
FaiI YATR 2 cut(s) 228, 267
FalI AAGNNNNNCTT 2 cut(s) 170, 202
FaqI GGGAC 1 cut(s) 86
FatI CATG 1 cut(s) 226
FauI CCCGC 2 cut(s) 106, 173
Fnu4HI GCNGC 3 cut(s) 13, 127, 145
FokI GGATG 3 cut(s) 65, 89, 218
Fsp4HI GCNGC 3 cut(s) 13, 127, 145
GlaI GCGC 1 cut(s) 270
GluI GCNGC 3 cut(s) 13, 127, 145
HaeIII GGCC 1 cut(s) 147
HapII CCGG 2 cut(s) 202, 232
HgaI GACGC 2 cut(s) 18, 118
HhaI GCGC 1 cut(s) 271
Hin1I GRCGYC 2 cut(s) 129, 258
Hin1II CATG 1 cut(s) 230
Hin6I GCGC 1 cut(s) 269
HinP1I GCGC 1 cut(s) 269
HinfI GANTC 1 cut(s) 289
HpaII CCGG 2 cut(s) 202, 232
HphI GGTGA 1 cut(s) 82
Hpy188III TCNNGA 2 cut(s) 178, 274
Hpy99I CGWCG 1 cut(s) 263
HpyCH4III ACNGT 1 cut(s) 244
HpyCH4IV ACGT 1 cut(s) 258
HpyF10VI GCNNNNNNNGC 3 cut(s) 21, 126, 277
HpyF3I CTNAG 1 cut(s) 68
HpySE526I ACGT 1 cut(s) 258
Hsp92I GRCGYC 2 cut(s) 129, 258
Hsp92II CATG 1 cut(s) 230
HspAI GCGC 1 cut(s) 269
KspI CCGCGG 1 cut(s) 169
Kzo9I GATC 2 cut(s) 180, 198
LmnI GCTCC 1 cut(s) 44
LpnPI CCDG 6 cut(s) 68, 95, 98, 215, 245, 266
LweI GCATC 1 cut(s) 196
MaeII ACGT 1 cut(s) 258
MaeIII GTNAC 2 cut(s) 211, 244
MalI GATC 2 cut(s) 182, 200
MboI GATC 2 cut(s) 180, 198
MboII GAAGA 2 cut(s) 175, 250
MflI RGATCY 1 cut(s) 180
MhlI GDGCHC 1 cut(s) 177
MlyI GAGTC 1 cut(s) 283
MnlI CCTC 8 cut(s) 14, 35, 45, 63, 151, 158, 228, 270
MspA1I CMGCKG 2 cut(s) 144, 168
MspI CCGG 2 cut(s) 202, 232
MspR9I CCNGG 3 cut(s) 83, 203, 232
MvaI CCWGG 1 cut(s) 83
MvnI CGCG 2 cut(s) 12, 168
MwoI GCNNNNNNNGC 3 cut(s) 21, 126, 277
NciI CCSGG 2 cut(s) 203, 232
NdeII GATC 2 cut(s) 180, 198
NlaIII CATG 1 cut(s) 230
NlaIV GGNNCC 1 cut(s) 46
NmuCI GTSAC 2 cut(s) 211, 244
NspI RCATGY 1 cut(s) 230
PaeI GCATGC 1 cut(s) 230
PflFI GACNNNGTC 1 cut(s) 260
PfoI TCCNGGA 1 cut(s) 201
PkrI GCNGC 3 cut(s) 14, 128, 146
PleI GAGTC 1 cut(s) 283
PpsI GAGTC 1 cut(s) 283
Psp6I CCWGG 1 cut(s) 81
PspGI CCWGG 1 cut(s) 81
PspN4I GGNNCC 1 cut(s) 46
PsuI RGATCY 1 cut(s) 180
PsyI GACNNNGTC 1 cut(s) 260
RsaI GTAC 1 cut(s) 296
RsaNI GTAC 1 cut(s) 295
SacII CCGCGG 1 cut(s) 169
SatI GCNGC 3 cut(s) 13, 127, 145
Sau3AI GATC 2 cut(s) 180, 198
SchI GAGTC 1 cut(s) 283
ScrFI CCNGG 3 cut(s) 83, 203, 232
SduI GDGCHC 1 cut(s) 177
SetI ASST 2 cut(s) 66, 261
SfaNI GCATC 1 cut(s) 196
Sfr303I CCGCGG 1 cut(s) 169
SgrBI CCGCGG 1 cut(s) 169
SmlI CTYRAG 2 cut(s) 176, 272
SmoI CTYRAG 2 cut(s) 176, 272
SphI GCATGC 1 cut(s) 230
SsiI CCGC 7 cut(s) 12, 99, 106, 126, 144, 166, 168
StyD4I CCNGG 3 cut(s) 81, 201, 230
TaaI ACNGT 1 cut(s) 244
TaiI ACGT 1 cut(s) 261
TauI GCSGC 3 cut(s) 15, 129, 147
TseFI GTSAC 2 cut(s) 211, 244
Tsp45I GTSAC 2 cut(s) 211, 244
Tth111I GACNNNGTC 1 cut(s) 260
XceI RCATGY 1 cut(s) 230
XcmI CCANNNNNNNNNTGG 1 cut(s) 109
ZraI GACGTC 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.