Rh7CG089600

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0000524
Physical Location & Seq
Forward (+)
6754845 .. 6755156
312 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG089600.1

Sequence Viewer

Length: 312 bp
ATGTCAGGAAGAGGAAAGGGAGGGAAGGGGTTGGGGAAAGGCGGAGCAAAGAGGCACAGGAAGGTGCTGAGGGACAACATCCAGGGCATCACCAAGCCTGCGATTCGGAGGCTCGCTCGAAGAGGAGGAGTGAAGCGCATTAGTGGGCTTATCTATGAAGAAACCAGAGGGGTGCTCAAGATCTTCTTGGAGAATGTGATTCGCGACGCGGTGACTTATACTGAGCATGCTAGGAGGAAGACTGTGACCGCCATGGATGTCGTCTATGCTCTCAAGAGGCAGGGCCGAACCCTCTATGGTTTCGGTGGTTAG

Protein Analysis

103

Amino Acids

11.41

Weight (kDa)

11.48

Isoelectric Point (pI)

45.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CENP-T_C PF15511 44 - 96 3.4e-08 Centromere kinetochore component CENP-T histone fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000524)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07660 AT1G07820 AT1G07820 AT2G28740 AT3G45930 AT3G46320 AT3G53730 AT5G59690 AT5G59970 AT5G59970
malus_domestica MD03G1097000.v1.1 MD03G1105400.v1.1 MD04G1124800.v1.1 MD06G1122500.v1.1 MD07G1184400.v1.1 MD07G1194500.v1.1 MD11G1143500.v1.1 MD12G1139800.v1.1 MD14G1139400.v1.1 MD15G1139200.v1.1
rosa_chinensis RchiOBHm_Chr1g0364991 RchiOBHm_Chr3g0467051 RchiOBHm_Chr5g0062221 RchiOBHm_Chr5g0063251 RchiOBHm_Chr5g0063281 RchiOBHm_Chr5g0063731 RchiOBHm_Chr7g0187711
rosa_laevigata RLG00000024533
rosa_multiflora Rmu_sc0004483.1_g000025 Rmu_sc0005045.1_g000017 Rmu_sc0005045.1_g000024 Rmu_sc0005638.1_g000003 Rmu_sc0042820.1_g000002 Rmu_sc0042820.1_g000003 Rmu_ssc0000112.1_g000021 Rmu_ssc0000309.1_g000047 Rmu_ssc0000409.1_g000022
rosa_roxburghii Rroxscaffold_1G00017140 Rroxscaffold_1G00017150 Rroxscaffold_1G00017430 Rroxscaffold_1G00017460 Rroxscaffold_4G00292020 Rroxscaffold_6G00413990
rosa_rugosa Rorug01G0318800 Rorug01G0329200 Rorug03G0085000 Rorug05G0347300 Rorug05G0355600 Rorug05G0355800 Rorug05G0358800 Rorug05G0358800 Rorug05G0358900 Rorug06G0483800
rosa_samantha Rh1AG327700 Rh1BG289000 Rh1CG305700 Rh1DG320500 Rh3AG133000 Rh3BG154000 Rh3CG154000 Rh3DG155100 Rh5AG407800 Rh5AG417800 Rh5BG421500 Rh5BG430300 Rh5BG433000 Rh5BG433200 Rh5CG445700 Rh5CG456200 Rh5CG456400 Rh5DG436500 Rh5DG443700 Rh5DG443900 Rh5DG446400 Rh5DG446600 Rh7BG090600 Rh7CG089600 Rh7DG091100
rosa_wichuraiana Rw1G029050 Rw3G012590 Rw5G038430 Rw5G039030 Rw5G039230 Rw5G039250 Rw7G007670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 204, 209
AciI CCGC 3 cut(s) 42, 209, 249
AjnI CCWGG 1 cut(s) 81
Alw21I GWGCWC 1 cut(s) 177
AoxI GGCC 1 cut(s) 283
AspLEI GCGC 1 cut(s) 138
AspS9I GGNCC 1 cut(s) 283
AsuHPI GGTGA 2 cut(s) 82, 223
BbsI GAAGAC 1 cut(s) 245
Bbv12I GWGCWC 1 cut(s) 177
BbvCI CCTCAGC 1 cut(s) 68
BciT130I CCWGG 1 cut(s) 83
BfaI CTAG 1 cut(s) 231
BglII AGATCT 1 cut(s) 180
Bme1390I CCNGG 1 cut(s) 83
BmgT120I GGNCC 1 cut(s) 283
BmrFI CCNGG 1 cut(s) 83
BmsI GCATC 1 cut(s) 96
BpiI GAAGAC 1 cut(s) 245
BplI GAGNNNNNCTC 4 cut(s) 100, 132, 159, 191
Bpu10I CCTNAGC 1 cut(s) 68
BpuEI CTTGAG 2 cut(s) 161, 257
BsaJI CCNNGG 2 cut(s) 82, 252
BseBI CCWGG 1 cut(s) 83
BseDI CCNNGG 2 cut(s) 82, 252
BseGI GGATG 2 cut(s) 78, 262
BseMII CTCAG 2 cut(s) 59, 213
BseRI GAGGAG 2 cut(s) 138, 141
Bsh1236I CGCG 2 cut(s) 204, 209
BshFI GGCC 1 cut(s) 285
BsiHKAI GWGCWC 1 cut(s) 177
BslFI GGGAC 1 cut(s) 86
BsmFI GGGAC 1 cut(s) 86
BsnI GGCC 1 cut(s) 285
Bsp1286I GDGCHC 1 cut(s) 177
Bsp143I GATC 1 cut(s) 180
Bsp19I CCATGG 1 cut(s) 252
Bsp68I TCGCGA 1 cut(s) 204
BspACI CCGC 3 cut(s) 42, 209, 249
BspANI GGCC 1 cut(s) 285
BspCNI CTCAG 2 cut(s) 60, 214
BspFNI CGCG 2 cut(s) 204, 209
BssECI CCNNGG 2 cut(s) 82, 252
BssMI GATC 1 cut(s) 180
BssT1I CCWWGG 1 cut(s) 252
Bst2UI CCWGG 1 cut(s) 83
Bst4CI ACNGT 1 cut(s) 244
Bst6I CTCTTC 2 cut(s) 4, 115
BstC8I GCNNGC 3 cut(s) 99, 114, 228
BstDEI CTNAG 2 cut(s) 68, 222
BstDSI CCRYGG 1 cut(s) 252
BstF5I GGATG 2 cut(s) 78, 262
BstFNI CGCG 2 cut(s) 204, 209
BstHHI GCGC 1 cut(s) 138
BstKTI GATC 1 cut(s) 183
BstMBI GATC 1 cut(s) 180
BstNI CCWGG 1 cut(s) 83
BstNSI RCATGY 1 cut(s) 230
BstSCI CCNGG 1 cut(s) 81
BstUI CGCG 2 cut(s) 204, 209
BstV2I GAAGAC 1 cut(s) 245
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BsuRI GGCC 1 cut(s) 285
BtgI CCRYGG 1 cut(s) 252
BtsCI GGATG 2 cut(s) 78, 262
BtuMI TCGCGA 1 cut(s) 204
Cac8I GCNNGC 3 cut(s) 99, 114, 228
CfoI GCGC 1 cut(s) 138
Cfr13I GGNCC 1 cut(s) 283
CseI GACGC 1 cut(s) 215
CviAII CATG 2 cut(s) 227, 253
CviJI RGCY 4 cut(s) 97, 112, 148, 285
CviKI_1 RGCY 4 cut(s) 97, 112, 148, 285
DdeI CTNAG 2 cut(s) 68, 222
DpnI GATC 1 cut(s) 182
DpnII GATC 1 cut(s) 180
Eam1104I CTCTTC 2 cut(s) 4, 115
EarI CTCTTC 2 cut(s) 4, 115
EciI GGCGGA 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 252
EcoRII CCWGG 1 cut(s) 81
EcoT14I CCWWGG 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 252
FaeI CATG 2 cut(s) 230, 256
FaiI YATR 6 cut(s) 156, 219, 228, 254, 267, 297
FalI AAGNNNNNCTT 2 cut(s) 170, 202
FaqI GGGAC 1 cut(s) 86
FatI CATG 2 cut(s) 226, 252
FokI GGATG 2 cut(s) 65, 269
FspBI CTAG 1 cut(s) 231
GlaI GCGC 1 cut(s) 137
HaeIII GGCC 1 cut(s) 285
HgaI GACGC 1 cut(s) 215
HhaI GCGC 1 cut(s) 138
Hin1II CATG 2 cut(s) 230, 256
Hin6I GCGC 1 cut(s) 136
HinP1I GCGC 1 cut(s) 136
HinfI GANTC 2 cut(s) 103, 199
HphI GGTGA 2 cut(s) 82, 223
Hpy188I TCNGA 1 cut(s) 108
Hpy188III TCNNGA 4 cut(s) 6, 178, 203, 274
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 2 cut(s) 19, 55
HpyCH4III ACNGT 1 cut(s) 244
HpyF3I CTNAG 2 cut(s) 68, 222
Hsp92II CATG 2 cut(s) 230, 256
HspAI GCGC 1 cut(s) 136
Kzo9I GATC 1 cut(s) 180
LmnI GCTCC 1 cut(s) 44
LpnPI CCDG 6 cut(s) 43, 68, 95, 111, 178, 266
LweI GCATC 1 cut(s) 96
MaeI CTAG 1 cut(s) 231
MaeIII GTNAC 2 cut(s) 211, 244
MalI GATC 1 cut(s) 182
MboI GATC 1 cut(s) 180
MboII GAAGA 5 cut(s) 21, 132, 170, 175, 250
MflI RGATCY 1 cut(s) 180
MhlI GDGCHC 1 cut(s) 177
MspR9I CCNGG 1 cut(s) 83
MvaI CCWGG 1 cut(s) 83
MvnI CGCG 2 cut(s) 204, 209
NcoI CCATGG 1 cut(s) 252
NdeII GATC 1 cut(s) 180
NlaIII CATG 2 cut(s) 230, 256
NmuCI GTSAC 2 cut(s) 211, 244
NruI TCGCGA 1 cut(s) 204
NspI RCATGY 1 cut(s) 230
PaeI GCATGC 1 cut(s) 230
PfeI GAWTC 2 cut(s) 103, 199
Psp6I CCWGG 1 cut(s) 81
PspGI CCWGG 1 cut(s) 81
PspPI GGNCC 1 cut(s) 283
PsuI RGATCY 1 cut(s) 180
RruI TCGCGA 1 cut(s) 204
Sau3AI GATC 1 cut(s) 180
Sau96I GGNCC 1 cut(s) 283
ScrFI CCNGG 1 cut(s) 83
SduI GDGCHC 1 cut(s) 177
SetI ASST 1 cut(s) 66
SfaNI GCATC 1 cut(s) 96
SmlI CTYRAG 2 cut(s) 176, 272
SmoI CTYRAG 2 cut(s) 176, 272
SphI GCATGC 1 cut(s) 230
SsiI CCGC 3 cut(s) 42, 209, 249
SspMI CTAG 1 cut(s) 231
StyD4I CCNGG 1 cut(s) 81
StyI CCWWGG 1 cut(s) 252
TaaI ACNGT 1 cut(s) 244
TaqI TCGA 1 cut(s) 118
TfiI GAWTC 2 cut(s) 103, 199
TseFI GTSAC 2 cut(s) 211, 244
Tsp45I GTSAC 2 cut(s) 211, 244
TspDTI ATGAA 1 cut(s) 171
XceI RCATGY 1 cut(s) 230
XspI CTAG 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.