Rh5DG443700

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000524
Physical Location & Seq
Forward (+)
71000558 .. 71000869
312 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG443700.1

Sequence Viewer

Length: 312 bp
ATGTCAGGCCGCGGCAAGGGAGGCAAGGGTTTGGGCAAAGGAGGAGCCAAGCGTCACAGGAAGGTTCTGAGGGATAACATCCAGGGCATCACCAAGCCCGCCATTCGCCGTTTGGCTCGCAGAGGCGGCGTCAAGCGTATCTCCGGTCTCATCTACGAAGAGACCCGAGGTGTCCTCAAGATCTTCCTCGAGAACGTCATCCGTGACGCCGTCACCTACACGGAGCATGCCCGGAGGAAGACCGTCACCGCCATGGATGTCGTCTATGCTCTCAAGAGGCAGGGAAGGACCCTTTACGGATTTGGGGGTTAA

Protein Analysis

103

Amino Acids

11.41

Weight (kDa)

11.48

Isoelectric Point (pI)

45.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CENP-T_C PF15511 44 - 96 3.4e-08 Centromere kinetochore component CENP-T histone fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000524)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07660 AT1G07820 AT1G07820 AT2G28740 AT3G45930 AT3G46320 AT3G53730 AT5G59690 AT5G59970 AT5G59970
malus_domestica MD03G1097000.v1.1 MD03G1105400.v1.1 MD04G1124800.v1.1 MD06G1122500.v1.1 MD07G1184400.v1.1 MD07G1194500.v1.1 MD11G1143500.v1.1 MD12G1139800.v1.1 MD14G1139400.v1.1 MD15G1139200.v1.1
rosa_chinensis RchiOBHm_Chr1g0364991 RchiOBHm_Chr3g0467051 RchiOBHm_Chr5g0062221 RchiOBHm_Chr5g0063251 RchiOBHm_Chr5g0063281 RchiOBHm_Chr5g0063731 RchiOBHm_Chr7g0187711
rosa_laevigata RLG00000024533
rosa_multiflora Rmu_sc0004483.1_g000025 Rmu_sc0005045.1_g000017 Rmu_sc0005045.1_g000024 Rmu_sc0005638.1_g000003 Rmu_sc0042820.1_g000002 Rmu_sc0042820.1_g000003 Rmu_ssc0000112.1_g000021 Rmu_ssc0000309.1_g000047 Rmu_ssc0000409.1_g000022
rosa_roxburghii Rroxscaffold_1G00017140 Rroxscaffold_1G00017150 Rroxscaffold_1G00017430 Rroxscaffold_1G00017460 Rroxscaffold_4G00292020 Rroxscaffold_6G00413990
rosa_rugosa Rorug01G0318800 Rorug01G0329200 Rorug03G0085000 Rorug05G0347300 Rorug05G0355600 Rorug05G0355800 Rorug05G0358800 Rorug05G0358800 Rorug05G0358900 Rorug06G0483800
rosa_samantha Rh1AG327700 Rh1BG289000 Rh1CG305700 Rh1DG320500 Rh3AG133000 Rh3BG154000 Rh3CG154000 Rh3DG155100 Rh5AG407800 Rh5AG417800 Rh5BG421500 Rh5BG430300 Rh5BG433000 Rh5BG433200 Rh5CG445700 Rh5CG456200 Rh5CG456400 Rh5DG436500 Rh5DG443700 Rh5DG443900 Rh5DG446400 Rh5DG446600 Rh7BG090600 Rh7CG089600 Rh7DG091100
rosa_wichuraiana Rw1G029050 Rw3G012590 Rw5G038430 Rw5G039030 Rw5G039230 Rw5G039250 Rw7G007670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 12
AciI CCGC 5 cut(s) 10, 12, 99, 126, 249
AcyI GRCGYC 2 cut(s) 129, 207
AfiI CCNNNNNNNGG 1 cut(s) 16
AjnI CCWGG 1 cut(s) 81
Alw26I GTCTC 2 cut(s) 152, 155
Ama87I CYCGRG 2 cut(s) 165, 188
AoxI GGCC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 288
AsuC2I CCSGG 1 cut(s) 232
AsuHPI GGTGA 3 cut(s) 82, 205, 238
AvaI CYCGRG 2 cut(s) 165, 188
AvaII GGWCC 1 cut(s) 288
BbsI GAAGAC 1 cut(s) 245
BceAI ACGGC 2 cut(s) 93, 194
BciT130I CCWGG 1 cut(s) 83
BcnI CCSGG 1 cut(s) 232
BcoDI GTCTC 2 cut(s) 152, 155
BglII AGATCT 1 cut(s) 180
BisI GCNGC 3 cut(s) 10, 13, 127
BlsI GCNGC 3 cut(s) 11, 14, 128
Bme1390I CCNGG 2 cut(s) 83, 232
Bme18I GGWCC 1 cut(s) 288
BmeT110I CYCGRG 2 cut(s) 165, 188
BmgT120I GGNCC 1 cut(s) 288
BmiI GGNNCC 2 cut(s) 46, 290
BmrFI CCNGG 2 cut(s) 83, 232
BmsI GCATC 1 cut(s) 96
BpiI GAAGAC 1 cut(s) 245
BplI GAGNNNNNCTC 2 cut(s) 159, 191
BpuEI CTTGAG 2 cut(s) 161, 257
BpuMI CCSGG 1 cut(s) 232
BsaHI GRCGYC 2 cut(s) 129, 207
BsaI GGTCTC 2 cut(s) 152, 155
BsaJI CCNNGG 4 cut(s) 10, 82, 166, 252
BsaWI WCCGGW 1 cut(s) 143
Bsc4I CCNNNNNNNGG 1 cut(s) 16
BseBI CCWGG 1 cut(s) 83
BseDI CCNNGG 4 cut(s) 10, 82, 166, 252
BseGI GGATG 3 cut(s) 78, 198, 262
BseLI CCNNNNNNNGG 1 cut(s) 16
BseMII CTCAG 1 cut(s) 59
BseRI GAGGAG 1 cut(s) 57
Bsh1236I CGCG 1 cut(s) 12
BshFI GGCC 1 cut(s) 9
BsiHKCI CYCGRG 2 cut(s) 165, 188
BsiSI CCGG 2 cut(s) 144, 232
BslI CCNNNNNNNGG 1 cut(s) 16
BsmAI GTCTC 2 cut(s) 152, 155
BsnI GGCC 1 cut(s) 9
Bso31I GGTCTC 2 cut(s) 152, 155
BsoBI CYCGRG 2 cut(s) 165, 188
Bsp143I GATC 1 cut(s) 180
Bsp19I CCATGG 1 cut(s) 252
BspACI CCGC 5 cut(s) 10, 12, 99, 126, 249
BspANI GGCC 1 cut(s) 9
BspCNI CTCAG 1 cut(s) 60
BspFNI CGCG 1 cut(s) 12
BspLI GGNNCC 2 cut(s) 46, 290
BspTNI GGTCTC 2 cut(s) 152, 155
BssECI CCNNGG 4 cut(s) 10, 82, 166, 252
BssMI GATC 1 cut(s) 180
BssNI GRCGYC 2 cut(s) 129, 207
BssT1I CCWWGG 1 cut(s) 252
Bst2UI CCWGG 1 cut(s) 83
Bst4CI ACNGT 1 cut(s) 244
Bst6I CTCTTC 1 cut(s) 153
BstACI GRCGYC 2 cut(s) 129, 207
BstC8I GCNNGC 3 cut(s) 99, 118, 228
BstDEI CTNAG 1 cut(s) 68
BstDSI CCRYGG 2 cut(s) 10, 252
BstF5I GGATG 3 cut(s) 78, 198, 262
BstFNI CGCG 1 cut(s) 12
BstKTI GATC 1 cut(s) 183
BstMAI GTCTC 2 cut(s) 152, 155
BstMBI GATC 1 cut(s) 180
BstMWI GCNNNNNNNGC 2 cut(s) 21, 126
BstNI CCWGG 1 cut(s) 83
BstNSI RCATGY 1 cut(s) 230
BstSCI CCNGG 2 cut(s) 81, 230
BstUI CGCG 1 cut(s) 12
BstV2I GAAGAC 1 cut(s) 245
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BsuRI GGCC 1 cut(s) 9
BtgI CCRYGG 2 cut(s) 10, 252
BtsCI GGATG 3 cut(s) 78, 198, 262
Cac8I GCNNGC 3 cut(s) 99, 118, 228
Cfr13I GGNCC 1 cut(s) 288
Cfr42I CCGCGG 1 cut(s) 13
CseI GACGC 3 cut(s) 41, 118, 215
CviAII CATG 2 cut(s) 227, 253
CviJI RGCY 4 cut(s) 9, 47, 97, 116
CviKI_1 RGCY 4 cut(s) 9, 47, 97, 116
DdeI CTNAG 1 cut(s) 68
DpnI GATC 1 cut(s) 182
DpnII GATC 1 cut(s) 180
Eam1104I CTCTTC 1 cut(s) 153
EarI CTCTTC 1 cut(s) 153
Eco130I CCWWGG 1 cut(s) 252
Eco31I GGTCTC 2 cut(s) 152, 155
Eco47I GGWCC 1 cut(s) 288
Eco88I CYCGRG 2 cut(s) 165, 188
EcoO109I RGGNCCY 1 cut(s) 288
EcoRII CCWGG 1 cut(s) 81
EcoT14I CCWWGG 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 252
FaeI CATG 2 cut(s) 230, 256
FaiI YATR 3 cut(s) 228, 254, 267
FatI CATG 2 cut(s) 226, 252
FauI CCCGC 1 cut(s) 106
Fnu4HI GCNGC 3 cut(s) 10, 13, 127
FokI GGATG 3 cut(s) 65, 185, 269
Fsp4HI GCNGC 3 cut(s) 10, 13, 127
GluI GCNGC 3 cut(s) 10, 13, 127
HaeIII GGCC 1 cut(s) 9
HapII CCGG 2 cut(s) 144, 232
HgaI GACGC 3 cut(s) 41, 118, 215
Hin1I GRCGYC 2 cut(s) 129, 207
Hin1II CATG 2 cut(s) 230, 256
HpaII CCGG 2 cut(s) 144, 232
HphI GGTGA 3 cut(s) 82, 205, 238
Hpy188I TCNGA 1 cut(s) 69
Hpy188III TCNNGA 3 cut(s) 178, 190, 274
HpyAV CCTTC 2 cut(s) 55, 279
HpyCH4III ACNGT 1 cut(s) 244
HpyCH4IV ACGT 1 cut(s) 195
HpyF10VI GCNNNNNNNGC 2 cut(s) 21, 126
HpyF3I CTNAG 1 cut(s) 68
HpySE526I ACGT 1 cut(s) 195
Hsp92I GRCGYC 2 cut(s) 129, 207
Hsp92II CATG 2 cut(s) 230, 256
KspI CCGCGG 1 cut(s) 13
Kzo9I GATC 1 cut(s) 180
LmnI GCTCC 2 cut(s) 44, 223
LpnPI CCDG 6 cut(s) 43, 68, 95, 157, 245, 266
LweI GCATC 1 cut(s) 96
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 4 cut(s) 53, 203, 211, 244
MalI GATC 1 cut(s) 182
MboI GATC 1 cut(s) 180
MboII GAAGA 3 cut(s) 170, 175, 250
MflI RGATCY 1 cut(s) 180
MnlI CCTC 9 cut(s) 14, 35, 63, 116, 161, 185, 197, 228, 270
MseI TTAA 1 cut(s) 310
MslI CAYNNNNRTG 1 cut(s) 251
MspA1I CMGCKG 1 cut(s) 12
MspI CCGG 2 cut(s) 144, 232
MspR9I CCNGG 2 cut(s) 83, 232
MvaI CCWGG 1 cut(s) 83
MvnI CGCG 1 cut(s) 12
MwoI GCNNNNNNNGC 2 cut(s) 21, 126
NciI CCSGG 1 cut(s) 232
NcoI CCATGG 1 cut(s) 252
NdeII GATC 1 cut(s) 180
NlaIII CATG 2 cut(s) 230, 256
NlaIV GGNNCC 2 cut(s) 46, 290
NmuCI GTSAC 4 cut(s) 53, 203, 211, 244
NspI RCATGY 1 cut(s) 230
PaeI GCATGC 1 cut(s) 230
PaeR7I CTCGAG 1 cut(s) 188
PflFI GACNNNGTC 1 cut(s) 209
PkrI GCNGC 3 cut(s) 11, 14, 128
PpuMI RGGWCCY 1 cut(s) 288
Psp5II RGGWCCY 1 cut(s) 288
Psp6I CCWGG 1 cut(s) 81
PspGI CCWGG 1 cut(s) 81
PspN4I GGNNCC 2 cut(s) 46, 290
PspPI GGNCC 1 cut(s) 288
PspPPI RGGWCCY 1 cut(s) 288
PsuI RGATCY 1 cut(s) 180
PsyI GACNNNGTC 1 cut(s) 209
RseI CAYNNNNRTG 1 cut(s) 251
SacII CCGCGG 1 cut(s) 13
SaqAI TTAA 1 cut(s) 310
SatI GCNGC 3 cut(s) 10, 13, 127
Sau3AI GATC 1 cut(s) 180
Sau96I GGNCC 1 cut(s) 288
ScrFI CCNGG 2 cut(s) 83, 232
SetI ASST 4 cut(s) 66, 172, 198, 218
SfaNI GCATC 1 cut(s) 96
Sfr274I CTCGAG 1 cut(s) 188
Sfr303I CCGCGG 1 cut(s) 13
SgrBI CCGCGG 1 cut(s) 13
SinI GGWCC 1 cut(s) 288
SlaI CTCGAG 1 cut(s) 188
SmiMI CAYNNNNRTG 1 cut(s) 251
SmlI CTYRAG 3 cut(s) 176, 188, 272
SmoI CTYRAG 3 cut(s) 176, 188, 272
SphI GCATGC 1 cut(s) 230
SsiI CCGC 5 cut(s) 10, 12, 99, 126, 249
StyD4I CCNGG 2 cut(s) 81, 230
StyI CCWWGG 1 cut(s) 252
TaaI ACNGT 1 cut(s) 244
TaiI ACGT 1 cut(s) 198
TaqI TCGA 1 cut(s) 189
TauI GCSGC 3 cut(s) 12, 15, 129
Tru1I TTAA 1 cut(s) 310
Tru9I TTAA 1 cut(s) 310
TseFI GTSAC 4 cut(s) 53, 203, 211, 244
Tsp45I GTSAC 4 cut(s) 53, 203, 211, 244
TspGWI ACGGA 3 cut(s) 191, 236, 312
Tth111I GACNNNGTC 1 cut(s) 209
VpaK11BI GGWCC 1 cut(s) 288
XceI RCATGY 1 cut(s) 230
XcmI CCANNNNNNNNNTGG 1 cut(s) 109
XhoI CTCGAG 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.