Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001577

Physical Location & Seq

Genomic Coordinates Reverse (-)

28547545 .. 28553856

Gene Structure 6312 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1197500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3312 bp

ATGTCGTCGGAGAAGCTCCTTGCCACCGGTTTTGTGCAGTCCGATGCAGTGAATATGAATGTGGGGTCTTTCTCCTTCACCCCGATTTTCGAAGAGCTTCCGGTGGCCACCATCGTGTCGGTTTCGCGGCCCGAAACCGGAGATATTAGCCCCATGCTTTTGTCTTACACCATTGAGTTTCAGTACAAGCAGGCAAAGCTCTCTCTTTCTCTGTGTAATTTGTATCTGTATGAACTGTTTGGGATGTTCAAGTGGCAGTTGCTGAAGAAAGCGTCACAGGTTCTCTATTTACATTTTGCTTTGAAGAAACGCGCGATAATTGAGGAGTTTCATGAGAAACAAGAACAGGTTAAAGAATGGCTGCATAGTATAGGAATAGTTGATCAGACAGCGGTAGTGCAAGATGATGACGAACCAGATGATGGGGCGGTCCCTTTACATCATGACGAAAGTGTTAGAAACAGAGATGTTCCATCTCGTGCTGCTCTTCCCATCCTCCGTCCAGCATTAGGAGGCCAGCAGTCCATTTCCGACAGAGGGAAGGTTGCAATGCAGGGTTATTTGAACCATTTTTTGGGGAACATGGATCTAGTCAATTCTCGAGAGGTCTGCAAATTTTTGGAGGTGTCGAAGCTCTCCTTTTTACAGGAGTATGGACCAAAGTTGAAAGAAGGTTATGTTATGGTGAAGCATCTACCAAAAATTACCGGGGCTAACTCTGATGTGAAATCATGTGCATACTTATGTCTTGATTGTTGCAGCAACAACTGGCAAAAGGTGTGGGCTGTTTTAAAACCTGGGTTCCTGGCCTTACTGGAGGATCCTTTTGACACCGAACTTTTAGACATTATTGTTTTCAATGTACTGCCAGATTCCAAAGAAATTGGACAGTCTAAAATATATTTGGCTAATCAAATAAAGGAACGTAACCCTTTGCGCTATACATTTAGGGTTGCTTGTGGAAATCAAAGCTTAAGGTTAAGAACCACTAGCAATGCTAAGGTTAAGGGATGGGTTACTGCAATAAATGATGCTGTTTCAAGGCCTCATGAAGGTTGGTGTCATCCTCATCGGTTTGGTTCCTTTGCTCCTCCAAGGGGTTTGGCCGATGACGGGAGTCAAGCTCAATGGTTTGTAGATGGAGAAGCAGCATTTGAAGCTATTGCTACTTCTATTGAAGGTGCAAAATCCGAGATTTTCATTACTGGCTGGTGGCTTTGCCCAGAACTGTATCTTAGACGTCCGTTTCATAGCAATCCATCTTCTCGGCTTGATGCATTACTAGAAGAAAAGGCTAAGCAAGGAGTTCAGATTTTCATTCTTCTCTACAAGGAGGTTTCTATTGCCTTAAAAATCAACAGTTCTTACAGCAAGAGATTGCTTTCTAACATTCATGAGAATGTGAGAGTAATGCGCTATCCTGACCGTTTCCCCACAGGCATTTATTTATGGTCACATCATGAAAAACTTGTCATTGTTGATTACCAGATTTGCTTCATGGGAGGATTAGACTTGTGCTTTGGCCGCTATGACACGGTTGAACACAAAGTGGGTGACTGCCCTCCTCATATATGGCCGGGAAAGGATTATTATAACCCAAGAGAATCTGAACCAAATTCTTGGGAGGACGCAATGCAAGATGAATTGGACCGTGAAAAGTATCCGCGTATGCCATGGCATGATGTCCATTGTGCTCTTTGGGGACCGCCCTGTCGTGACATTGCTCGGCACTTTGTTCAGCGTTGGAACCATGCTAAGAGAAACAAAGCTCCAAATGAACAAACAATTCCGCTACTTATGCCTCAGCACCACATGGTTCTCCCCCACTACTTGGGAAGAAGTAGCGAGATAGACATTGAGAAGAAAAACAAAGAAGAAAACCAGAATGGAATCTGCAGAGAGAACTCTTTTTCCTCGCTATCACCAGTGCAAAATATTCCATTGCTTTTGCCTCAAGAAGATGGTTTGGATGCTCCTATTGAAAACCAAAAGTTAAGCGCTGTGAACTTGAATCATAATCTTTTTGACCAGCCAGCTGATGATCTAGACCCTGATACACAGATGAAAGGTTTCACAGATGACCTTCATTCTAAGGATCTCAAAAGTGAAGCAAATTTAAACATGGCACATTCTGGCTCGACAACTTCAGATGAATCGTCAGAAAGTTCAGAGGGAGGTGATCATGCTGTTGCTGCTGATGATTACGGACAAATAGGTCCTCATACTGCATGCCATTGTCAGGTTGTGAGAAGTGTGAGCCAGTGGTCTGCTGGATCTAGCCAAACTGAAGATAGCATACACAGCGCTTATTGTTCTCTCATTGAGAACGCAGAACATTTTGTCTATATTGAGAATCAATTTTTCATATCAGGTCTTTCAGGGGATGAGATTATACAGAATCGTGTCCTGGAAACGTTGTACAGGCGTATTGTGCTAGCATACAAAGAACAAAAGTGTTTTAGGGTTATTGTTGTCATACCACTATTACCCGGCTTTCAGGGTGGTGTGGACGATGGTGGTGCAGCTACCGTTAGAGCCATAATGCATTGGCAGTACCGTACCATTAGCTGGGAAAAACACTCAATACTGCATAAGCTCAAAGTTAAGCTTGGTCCTAAGACACACGATTACATTTCTTTCTATGGTCTGAGGACATATGGAAGACTTTTCGAAGGTGGCCCTGTGGCAACAAGTCAGGTTTATGTACACAGCAAAGTGATGATAATAGATGATCGTATTTCCTTGATTGGATCATCTAATATTAATGACAGAAGTCTGCTCGGATCAAGAGACTCTGAGATTGGTGTAGTGCTTGAGGACAAGGAGTTTCTTGAATCATCTATGAATGGACAACCATGGAAGGCCGGAAAATTGGCGTATAGTCTCAGGTGCTCGCTGTGGTCCGAGCACCTTGGTCTCCAGGCAGGGGAGATAAATCAAATCAGTGATCCTGTATCAGACACAACATACAAGAATCTGTGGTTAGAAACTGCGAAGGAAAATTCAATTATTTACGAAGATGTCTTTTCTTGCATCCCTAATGATTCTATTCACTCAAGAGCTGCTCTCAGACAATGCAAGGCTCAATGGAAAGACAAACATGGCCACACAACGATCGATTTGGGAATAGCCCCCGAGAAGCTGTTGGAGTCCGGCAAGGATGGAGAGGCGAAAGAAACAGATCCGATGGAAAGACTGAAGCATGTAAGGGGGCATCTCGTTTCCTTCCCTTTAGAGTTTATGCAGCAAGAAGATTTGAGGCCAGTTTTCAATGAAAGTGAATTTTATACATCCCCTCAAGTATTTCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004620 GO:0004630 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0006629 GO:0006643 GO:0006644 GO:0006664 GO:0006793 GO:0006796 GO:0006950 GO:0006995 GO:0007154 GO:0007275 GO:0008081 GO:0008150 GO:0008152 GO:0008610 GO:0009056 GO:0009058 GO:0009247 GO:0009267 GO:0009395 GO:0009605 GO:0009719 GO:0009725 GO:0009733 GO:0009987 GO:0009991 GO:0010033 GO:0016036 GO:0016042 GO:0016298 GO:0016787 GO:0016788 GO:0019374 GO:0019375 GO:0019637 GO:0022622 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0032879 GO:0033554 GO:0042221 GO:0042578 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043562 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0046434 GO:0046467 GO:0048364 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051716 GO:0060627 GO:0065007 GO:0071496 GO:0071704 GO:0099402 GO:1901135 GO:1901137 GO:1901575 GO:1901576 GO:1903509

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

PH PLDc PLDc_2

Protein Analysis

1104

Amino Acids

125.0

Weight (kDa)

5.81

Isoelectric Point (pI)

43.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PLDc_2	PF13091	899 - 945	2.9e-08	PLD-like domain
PLDc	PF00614	902 - 925	2.3e-07	Phospholipase D Active site motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001577)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16785 AT3G16785 AT3G16785 AT3G16785 AT3G16785 AT3G16785
fragaria_vesca	FvH4_4g18810 FvH4_6g06940 FvH4_6g06940 FvH4_6g06940
malus_domestica	MD04G1197500.v1.1 MD12G1210300.v1.1 MD13G1162600.v1.1 MD16G1162400.v1.1
prunus_persica	Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.6G317900_v2.0.a1 Prupe.6G317900_v2.0.a1 Prupe.6G317900_v2.0.a1
pyrus_communis	pycom04g17480 pycom13g13890 pycom16g13770
rosa_chinensis	RchiOBHm_Chr3g0456481 RchiOBHm_Chr4g0423621
rosa_laevigata	RLG00000007541 RLG00000025302
rosa_multiflora	Rmu_co8413375.1_g000001 Rmu_sc0000464.1_g000017 Rmu_sc0002607.1_g000011 Rmu_sc0008373.1_g000012 Rmu_sc0016309.1_g000001
rosa_roxburghii	Rroxscaffold_5G00365830 Rroxscaffold_6G00422720
rosa_rugosa	Rorug03G0013400 Rorug03G0013400 Rorug03G0013400 Rorug04G0189100
rosa_samantha	Rh3AG073700 Rh3BG075600 Rh3DG076900 Rh4AG248700 Rh4BG254000 Rh4DG249000
rosa_wichuraiana	Rw3G006320 Rw4G021300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1593
AasI	GACNNNNNNGTC	2 cut(s)	1710, 2214
AatII	GACGTC	1 cut(s)	1243
AccB7I	CCANNNNNTGG	4 cut(s)	422, 574, 1831, 2568
AccII	CGCG	4 cut(s)	127, 312, 314, 1666
AciI	CCGC	7 cut(s)	127, 392, 428, 1525, 1664, 1706, 1790
AclI	AACGTT	1 cut(s)	2414
AclWI	GGATC	9 cut(s)	594, 815, 828, 2103, 2281, 2758, 2791, 2942, 3176
AcoI	YGGCCR	5 cut(s)	105, 1104, 1522, 1574, 3103
AcsI	RAATTY	5 cut(s)	614, 1615, 2113, 3001, 3281
AcuI	CTGAAG	4 cut(s)	284, 2130, 2307, 3218
AcyI	GRCGYC	1 cut(s)	1240
AdeI	CACNNNGTG	1 cut(s)	1549
AfaI	GTAC	6 cut(s)	185, 864, 2420, 2555, 2560, 2706
AfeI	AGCGCT	2 cut(s)	1999, 2305
AflII	CTTAAG	1 cut(s)	973
AgeI	ACCGGT	1 cut(s)	26
AjnI	CCWGG	4 cut(s)	796, 804, 2406, 2919
AjuI	GAANNNNNNNTTGG	2 cut(s)	557, 589
AleI	CACNNNNGTG	1 cut(s)	113
AloI	GAACNNNNNNTCC	2 cut(s)	1895, 1927
Alw21I	GWGCWC	3 cut(s)	1696, 2894, 2910
Alw26I	GTCTC	3 cut(s)	2784, 2888, 2921
AlwI	GGATC	9 cut(s)	594, 815, 828, 2103, 2281, 2758, 2791, 2942, 3176
AlwNI	CAGNNNCTG	1 cut(s)	262
Ama87I	CYCGRG	2 cut(s)	600, 3134
Aor51HI	AGCGCT	2 cut(s)	1999, 2305
ApeKI	GCWGC	8 cut(s)	361, 482, 759, 1148, 2192, 2522, 3062, 3244
ApoI	RAATTY	5 cut(s)	614, 1615, 2113, 3001, 3281
ArsI	GACNNNNNNTTYG	2 cut(s)	1502, 1534
AseI	ATTAAT	1 cut(s)	2763
AsiGI	ACCGGT	1 cut(s)	26
Asp700I	GAANNNNTTC	2 cut(s)	96, 2069
AspLEI	GCGC	5 cut(s)	314, 939, 1416, 2000, 2306
AspS9I	GGNCC	9 cut(s)	129, 430, 656, 1648, 1703, 2216, 2612, 2678, 2901
AsuC2I	CCSGG	3 cut(s)	709, 1578, 2490
AsuHPI	GGTGA	5 cut(s)	70, 697, 1565, 1914, 2189
AsuII	TTCGAA	2 cut(s)	90, 2670
AsuNHI	GCTAGC	1 cut(s)	2434
AvaI	CYCGRG	2 cut(s)	600, 3134
AvaII	GGWCC	7 cut(s)	430, 656, 1648, 1703, 2216, 2612, 2901
BalI	TGGCCA	2 cut(s)	107, 3105
BamHI	GGATCC	1 cut(s)	820
BarI	GAAGNNNNNNTAC	2 cut(s)	2280, 2312
BauI	CACGAG	1 cut(s)	477
BbsI	GAAGAC	1 cut(s)	2668
Bbv12I	GWGCWC	3 cut(s)	1696, 2894, 2910
BbvCI	CCTCAGC	1 cut(s)	1803
BbvI	GCAGC	8 cut(s)	348, 469, 771, 1160, 2179, 2534, 3049, 3256
BcgI	CGANNNNNNTGC	4 cut(s)	591, 625, 2501, 2535
BciT130I	CCWGG	4 cut(s)	798, 806, 2408, 2921
BciVI	GTATCC	1 cut(s)	1671
BclI	TGATCA	2 cut(s)	382, 2179
BcnI	CCSGG	3 cut(s)	709, 1578, 2490
BcoDI	GTCTC	3 cut(s)	2784, 2888, 2921
BfaI	CTAG	6 cut(s)	590, 990, 1283, 2045, 2277, 2435
BfmI	CTRYAG	1 cut(s)	1894
BfoI	RGCGCY	2 cut(s)	2001, 2307
BfrI	CTTAAG	1 cut(s)	973
BfuI	GTATCC	1 cut(s)	1671
BlpI	GCTNAGC	1 cut(s)	1296
Bme1390I	CCNGG	7 cut(s)	709, 798, 806, 1578, 2408, 2490, 2921
Bme18I	GGWCC	7 cut(s)	430, 656, 1648, 1703, 2216, 2612, 2901
BmeT110I	CYCGRG	2 cut(s)	600, 3134
BmgT120I	GGNCC	9 cut(s)	129, 430, 656, 1648, 1703, 2216, 2612, 2678, 2901
BmiI	GGNNCC	6 cut(s)	432, 803, 822, 1081, 1704, 1748
BmrFI	CCNGG	7 cut(s)	709, 798, 806, 1578, 2408, 2490, 2921
BmsI	GCATC	7 cut(s)	34, 700, 1021, 1264, 1960, 3042, 3223
BmtI	GCTAGC	1 cut(s)	2438
BoxI	GACNNNNGTC	2 cut(s)	1116, 2772
BpiI	GAAGAC	1 cut(s)	2668
BplI	GAGNNNNNCTC	4 cut(s)	1108, 1140, 3051, 3083
BpmI	CTGGAG	2 cut(s)	836, 2903
Bpu10I	CCTNAGC	2 cut(s)	999, 1803
Bpu1102I	GCTNAGC	1 cut(s)	1296
Bpu14I	TTCGAA	2 cut(s)	90, 2670
BpuEI	CTTGAG	4 cut(s)	1938, 2834, 3040, 3282
BpuMI	CCSGG	3 cut(s)	709, 1578, 2490
Bsa29I	ATCGAT	1 cut(s)	3117
BsaHI	GRCGYC	1 cut(s)	1240
BsaI	GGTCTC	1 cut(s)	2921
BsaJI	CCNNGG	6 cut(s)	708, 797, 1094, 1673, 2855, 2911
BsaWI	WCCGGW	3 cut(s)	26, 100, 137
Bse118I	RCCGGY	1 cut(s)	26
Bse1I	ACTGG	6 cut(s)	773, 819, 1210, 1925, 2260, 3263
Bse3DI	GCAATG	5 cut(s)	555, 1000, 1638, 1719, 1940
BseBI	CCWGG	4 cut(s)	798, 806, 2408, 2921
BseCI	ATCGAT	1 cut(s)	3117
BseDI	CCNNGG	6 cut(s)	708, 797, 1094, 1673, 2855, 2911
BseGI	GGATG	9 cut(s)	249, 492, 1016, 1063, 1975, 2389, 3033, 3166, 3290
BseMI	GCAATG	5 cut(s)	555, 1000, 1638, 1719, 1940
BseMII	CTCAG	5 cut(s)	1817, 2639, 2787, 2899, 3082
BseNI	ACTGG	6 cut(s)	773, 819, 1210, 1925, 2260, 3263
BseRI	GAGGAG	3 cut(s)	338, 1080, 1554
BseXI	GCAGC	8 cut(s)	348, 469, 771, 1160, 2179, 2534, 3049, 3256
BseYI	CCCAGC	1 cut(s)	2568
BsgI	GTGCAG	2 cut(s)	56, 2541
Bsh1236I	CGCG	4 cut(s)	127, 312, 314, 1666
Bsh1285I	CGRYCG	1 cut(s)	3117
BshTI	ACCGGT	1 cut(s)	26
BshVI	ATCGAT	1 cut(s)	3117
BsiEI	CGRYCG	1 cut(s)	3117
BsiHKAI	GWGCWC	3 cut(s)	1696, 2894, 2910
BsiHKCI	CYCGRG	2 cut(s)	600, 3134
BsiSI	CCGG	8 cut(s)	27, 101, 138, 708, 1577, 2490, 2865, 3153
BslFI	GGGAC	2 cut(s)	416, 1716
BsmAI	GTCTC	3 cut(s)	2784, 2888, 2921
BsmFI	GGGAC	2 cut(s)	416, 1716
Bso31I	GGTCTC	1 cut(s)	2921
BsoBI	CYCGRG	2 cut(s)	600, 3134
Bsp119I	TTCGAA	2 cut(s)	90, 2670
Bsp1286I	GDGCHC	3 cut(s)	1696, 2894, 2910
Bsp1407I	TGTACA	2 cut(s)	2418, 2704
Bsp1720I	GCTNAGC	1 cut(s)	1296
Bsp19I	CCATGG	2 cut(s)	1673, 2855
BspACI	CCGC	7 cut(s)	127, 392, 428, 1525, 1664, 1706, 1790
BspCNI	CTCAG	5 cut(s)	1816, 2640, 2788, 2898, 3081
BspDI	ATCGAT	1 cut(s)	3117
BspFNI	CGCG	4 cut(s)	127, 312, 314, 1666
BspHI	TCATGA	5 cut(s)	331, 442, 1048, 1393, 1459
BspLI	GGNNCC	6 cut(s)	432, 803, 822, 1081, 1704, 1748
BspMAI	CTGCAG	1 cut(s)	1898
BspOI	GCTAGC	1 cut(s)	2438
BspPI	GGATC	9 cut(s)	594, 815, 828, 2103, 2281, 2758, 2791, 2942, 3176
BspQI	GCTCTTC	2 cut(s)	87, 492
BspT104I	TTCGAA	2 cut(s)	90, 2670
BspTI	CTTAAG	1 cut(s)	973
BspTNI	GGTCTC	1 cut(s)	2921
BsrDI	GCAATG	5 cut(s)	555, 1000, 1638, 1719, 1940
BsrFI	RCCGGY	1 cut(s)	26
BsrGI	TGTACA	2 cut(s)	2418, 2704
BsrI	ACTGG	6 cut(s)	773, 819, 1210, 1925, 2260, 3263
BssAI	RCCGGY	1 cut(s)	26
BssECI	CCNNGG	6 cut(s)	708, 797, 1094, 1673, 2855, 2911
BssNI	GRCGYC	1 cut(s)	1240
BssSI	CACGAG	1 cut(s)	477
BssT1I	CCWWGG	4 cut(s)	1094, 1673, 2855, 2911
Bst2BI	CACGAG	1 cut(s)	477
Bst2UI	CCWGG	4 cut(s)	798, 806, 2408, 2921
Bst4CI	ACNGT	9 cut(s)	237, 891, 1230, 1361, 1427, 1537, 1652, 2530, 2558
Bst6I	CTCTTC	2 cut(s)	87, 492
BstACI	GRCGYC	1 cut(s)	1240
BstAFI	CTTAAG	1 cut(s)	973
BstAUI	TGTACA	2 cut(s)	2418, 2704
BstBI	TTCGAA	2 cut(s)	90, 2670
BstC8I	GCNNGC	6 cut(s)	192, 518, 2034, 2230, 2436, 2894
BstDSI	CCRYGG	2 cut(s)	1673, 2855
BstENI	CCTNNNNNAGG	1 cut(s)	1050
BstF5I	GGATG	9 cut(s)	249, 492, 1016, 1063, 1975, 2389, 3033, 3166, 3290
BstFNI	CGCG	4 cut(s)	127, 312, 314, 1666
BstH2I	RGCGCY	2 cut(s)	2001, 2307
BstHHI	GCGC	5 cut(s)	314, 939, 1416, 2000, 2306
BstMAI	GTCTC	3 cut(s)	2784, 2888, 2921
BstMCI	CGRYCG	1 cut(s)	3117
BstMWI	GCNNNNNNNGC	7 cut(s)	196, 1157, 1524, 1798, 2192, 2301, 2431
BstNI	CCWGG	4 cut(s)	798, 806, 2408, 2921
BstNSI	RCATGY	2 cut(s)	2232, 3206
BstPAI	GACNNNNGTC	2 cut(s)	1116, 2772
BstSCI	CCNGG	7 cut(s)	707, 796, 804, 1576, 2406, 2488, 2919
BstSFI	CTRYAG	1 cut(s)	1894
BstUI	CGCG	4 cut(s)	127, 312, 314, 1666
BstV1I	GCAGC	8 cut(s)	348, 469, 771, 1160, 2179, 2534, 3049, 3256
BstV2I	GAAGAC	1 cut(s)	2668
BstX2I	RGATCY	5 cut(s)	586, 820, 2095, 2273, 3181
BstXI	CCANNNNNNTGG	1 cut(s)	1620
BstYI	RGATCY	5 cut(s)	586, 820, 2095, 2273, 3181
Bsu15I	ATCGAT	1 cut(s)	3117
BsuI	GTATCC	1 cut(s)	1671
BsuTUI	ATCGAT	1 cut(s)	3117
BtgI	CCRYGG	2 cut(s)	1673, 2855
BtsCI	GGATG	9 cut(s)	249, 492, 1016, 1063, 1975, 2389, 3033, 3166, 3290
BtsI	GCAGTG	1 cut(s)	54
BtsIMutI	CAGTG	4 cut(s)	54, 1932, 2267, 2950
Cac8I	GCNNGC	6 cut(s)	192, 518, 2034, 2230, 2436, 2894
CaiI	CAGNNNCTG	1 cut(s)	262
CciI	TCATGA	5 cut(s)	331, 442, 1048, 1393, 1459
CfoI	GCGC	5 cut(s)	314, 939, 1416, 2000, 2306
Cfr10I	RCCGGY	1 cut(s)	26
Cfr13I	GGNCC	9 cut(s)	129, 430, 656, 1648, 1703, 2216, 2612, 2678, 2901
ClaI	ATCGAT	1 cut(s)	3117
CseI	GACGC	2 cut(s)	261, 1637
Csp6I	GTAC	6 cut(s)	184, 863, 2419, 2554, 2559, 2705
CspAI	ACCGGT	1 cut(s)	26
CspCI	CAANNNNNGTGG	4 cut(s)	13, 48, 761, 796
CviQI	GTAC	6 cut(s)	184, 863, 2419, 2554, 2559, 2705
DraI	TTTAAA	2 cut(s)	792, 2118
DraIII	CACNNNGTG	1 cut(s)	1549
DrdI	GACNNNNNNGTC	2 cut(s)	1710, 2214
DseDI	GACNNNNNNGTC	2 cut(s)	1710, 2214
EaeI	YGGCCR	5 cut(s)	105, 1104, 1522, 1574, 3103
Eam1104I	CTCTTC	2 cut(s)	87, 492
EarI	CTCTTC	2 cut(s)	87, 492
Eco130I	CCWWGG	4 cut(s)	1094, 1673, 2855, 2911
Eco147I	AGGCCT	1 cut(s)	1045
Eco31I	GGTCTC	1 cut(s)	2921
Eco47I	GGWCC	7 cut(s)	430, 656, 1648, 1703, 2216, 2612, 2901
Eco47III	AGCGCT	2 cut(s)	1999, 2305
Eco57I	CTGAAG	4 cut(s)	284, 2130, 2307, 3218
Eco88I	CYCGRG	2 cut(s)	600, 3134
EcoNI	CCTNNNNNAGG	1 cut(s)	1050
EcoO109I	RGGNCCY	1 cut(s)	2216
EcoRII	CCWGG	4 cut(s)	796, 804, 2406, 2919
EcoT14I	CCWWGG	4 cut(s)	1094, 1673, 2855, 2911
EcoT22I	ATGCAT	2 cut(s)	1279, 2547
ErhI	CCWWGG	4 cut(s)	1094, 1673, 2855, 2911
FalI	AAGNNNNNCTT	4 cut(s)	623, 655, 2592, 2624
FaqI	GGGAC	2 cut(s)	416, 1716
FauNDI	CATATG	1 cut(s)	2656
FbaI	TGATCA	2 cut(s)	382, 2179
FokI	GGATG	9 cut(s)	256, 479, 1023, 1050, 1982, 2396, 3020, 3173, 3277
FspBI	CTAG	6 cut(s)	590, 990, 1283, 2045, 2277, 2435
GlaI	GCGC	5 cut(s)	313, 938, 1415, 1999, 2305
GsaI	CCCAGC	1 cut(s)	2572
GsuI	CTGGAG	2 cut(s)	836, 2903
HaeII	RGCGCY	2 cut(s)	2001, 2307
HapII	CCGG	8 cut(s)	27, 101, 138, 708, 1577, 2490, 2865, 3153
HgaI	GACGC	2 cut(s)	261, 1637
HhaI	GCGC	5 cut(s)	314, 939, 1416, 2000, 2306
Hin1I	GRCGYC	1 cut(s)	1240
Hin6I	GCGC	5 cut(s)	312, 937, 1414, 1998, 2304
HinP1I	GCGC	5 cut(s)	312, 937, 1414, 1998, 2304
HindIII	AAGCTT	2 cut(s)	970, 2606
HpaII	CCGG	8 cut(s)	27, 101, 138, 708, 1577, 2490, 2865, 3153
HphI	GGTGA	5 cut(s)	70, 697, 1565, 1914, 2189
Hpy166II	GTNNAC	3 cut(s)	2005, 2509, 2707
Hpy8I	GTNNAC	3 cut(s)	2005, 2509, 2707
Hpy99I	CGWCG	1 cut(s)	10
HpyCH4III	ACNGT	9 cut(s)	237, 891, 1230, 1361, 1427, 1537, 1652, 2530, 2558
HpyCH4IV	ACGT	3 cut(s)	925, 1240, 2414
HpyF10VI	GCNNNNNNNGC	7 cut(s)	196, 1157, 1524, 1798, 2192, 2301, 2431
HpySE526I	ACGT	3 cut(s)	925, 1240, 2414
Hsp92I	GRCGYC	1 cut(s)	1240
HspAI	GCGC	5 cut(s)	312, 937, 1414, 1998, 2304
Ksp22I	TGATCA	2 cut(s)	382, 2179
LguI	GCTCTTC	2 cut(s)	87, 492
LmnI	GCTCC	4 cut(s)	21, 1093, 1774, 1978
Lsp1109I	GCAGC	8 cut(s)	348, 469, 771, 1160, 2179, 2534, 3049, 3256
LweI	GCATC	7 cut(s)	34, 700, 1021, 1264, 1960, 3042, 3223
MaeI	CTAG	6 cut(s)	590, 990, 1283, 2045, 2277, 2435
MaeII	ACGT	3 cut(s)	925, 1240, 2414
MaeIII	GTNAC	6 cut(s)	273, 926, 1015, 1452, 1553, 1715
MflI	RGATCY	5 cut(s)	586, 820, 2095, 2273, 3181
MhlI	GDGCHC	3 cut(s)	1696, 2894, 2910
MlsI	TGGCCA	2 cut(s)	107, 3105
MluNI	TGGCCA	2 cut(s)	107, 3105
MlyI	GAGTC	3 cut(s)	1126, 2786, 3158
MmeI	TCCRAC	3 cut(s)	555, 1724, 3126
Mox20I	TGGCCA	2 cut(s)	107, 3105
Mph1103I	ATGCAT	2 cut(s)	1279, 2547
MroXI	GAANNNNTTC	2 cut(s)	96, 2069
MscI	TGGCCA	2 cut(s)	107, 3105
MslI	CAYNNNNRTG	3 cut(s)	113, 742, 1398
Msp20I	TGGCCA	2 cut(s)	107, 3105
MspA1I	CMGCKG	2 cut(s)	392, 2036
MspCI	CTTAAG	1 cut(s)	973
MspI	CCGG	8 cut(s)	27, 101, 138, 708, 1577, 2490, 2865, 3153
MspR9I	CCNGG	7 cut(s)	709, 798, 806, 1578, 2408, 2490, 2921
MvaI	CCWGG	4 cut(s)	798, 806, 2408, 2921
MvnI	CGCG	4 cut(s)	127, 312, 314, 1666
MwoI	GCNNNNNNNGC	7 cut(s)	196, 1157, 1524, 1798, 2192, 2301, 2431
NciI	CCSGG	3 cut(s)	709, 1578, 2490
NcoI	CCATGG	2 cut(s)	1673, 2855
NdeI	CATATG	1 cut(s)	2656
NheI	GCTAGC	1 cut(s)	2434
NlaIV	GGNNCC	6 cut(s)	432, 803, 822, 1081, 1704, 1748
NmeAIII	GCCGAG	2 cut(s)	1246, 1705
NmuCI	GTSAC	4 cut(s)	273, 1452, 1553, 1715
NsiI	ATGCAT	2 cut(s)	1279, 2547
NspI	RCATGY	2 cut(s)	2232, 3206
NspV	TTCGAA	2 cut(s)	90, 2670
OliI	CACNNNNGTG	1 cut(s)	113
PaeI	GCATGC	1 cut(s)	2232
PaeR7I	CTCGAG	1 cut(s)	600
PagI	TCATGA	5 cut(s)	331, 442, 1048, 1393, 1459
PceI	AGGCCT	1 cut(s)	1045
PciSI	GCTCTTC	2 cut(s)	87, 492
PdmI	GAANNNNTTC	2 cut(s)	96, 2069
PflMI	CCANNNNNTGG	4 cut(s)	422, 574, 1831, 2568
PfoI	TCCNGGA	1 cut(s)	2406
PinAI	ACCGGT	1 cut(s)	26
Ple19I	CGATCG	1 cut(s)	3117
PleI	GAGTC	3 cut(s)	1125, 2786, 3157
PpsI	GAGTC	3 cut(s)	1125, 2786, 3157
PpuMI	RGGWCCY	1 cut(s)	2216
PshAI	GACNNNNGTC	2 cut(s)	1116, 2772
PshBI	ATTAAT	1 cut(s)	2763
PsiI	TTATAA	1 cut(s)	1593
Psp1406I	AACGTT	1 cut(s)	2414
Psp5II	RGGWCCY	1 cut(s)	2216
Psp6I	CCWGG	4 cut(s)	796, 804, 2406, 2919
PspFI	CCCAGC	1 cut(s)	2568
PspGI	CCWGG	4 cut(s)	796, 804, 2406, 2919
PspN4I	GGNNCC	6 cut(s)	432, 803, 822, 1081, 1704, 1748
PspPI	GGNCC	9 cut(s)	129, 430, 656, 1648, 1703, 2216, 2612, 2678, 2901
PspPPI	RGGWCCY	1 cut(s)	2216
PstI	CTGCAG	1 cut(s)	1898
PstNI	CAGNNNCTG	1 cut(s)	262
PsuI	RGATCY	5 cut(s)	586, 820, 2095, 2273, 3181
PvuI	CGATCG	1 cut(s)	3117
PvuII	CAGCTG	1 cut(s)	2036
RsaI	GTAC	6 cut(s)	185, 864, 2420, 2555, 2560, 2706
RsaNI	GTAC	6 cut(s)	184, 863, 2419, 2554, 2559, 2705
RseI	CAYNNNNRTG	3 cut(s)	113, 742, 1398
SapI	GCTCTTC	2 cut(s)	87, 492
Sau96I	GGNCC	9 cut(s)	129, 430, 656, 1648, 1703, 2216, 2612, 2678, 2901
SchI	GAGTC	3 cut(s)	1126, 2786, 3158
ScrFI	CCNGG	7 cut(s)	709, 798, 806, 1578, 2408, 2490, 2921
SduI	GDGCHC	3 cut(s)	1696, 2894, 2910
SfaNI	GCATC	7 cut(s)	34, 700, 1021, 1264, 1960, 3042, 3223
SfcI	CTRYAG	1 cut(s)	1894
Sfr274I	CTCGAG	1 cut(s)	600
SfuI	TTCGAA	2 cut(s)	90, 2670
SinI	GGWCC	7 cut(s)	430, 656, 1648, 1703, 2216, 2612, 2901
SlaI	CTCGAG	1 cut(s)	600
SmiMI	CAYNNNNRTG	3 cut(s)	113, 742, 1398
SmlI	CTYRAG	6 cut(s)	600, 973, 1953, 2813, 3055, 3297
SmoI	CTYRAG	6 cut(s)	600, 973, 1953, 2813, 3055, 3297
SphI	GCATGC	1 cut(s)	2232
SseBI	AGGCCT	1 cut(s)	1045
SsiI	CCGC	7 cut(s)	127, 392, 428, 1525, 1664, 1706, 1790
SspI	AATATT	2 cut(s)	1936, 2761
SspMI	CTAG	6 cut(s)	590, 990, 1283, 2045, 2277, 2435
StuI	AGGCCT	1 cut(s)	1045
StyD4I	CCNGG	7 cut(s)	707, 796, 804, 1576, 2406, 2488, 2919
StyI	CCWWGG	4 cut(s)	1094, 1673, 2855, 2911
TaaI	ACNGT	9 cut(s)	237, 891, 1230, 1361, 1427, 1537, 1652, 2530, 2558
TaiI	ACGT	3 cut(s)	928, 1243, 2417
TaqI	TCGA	6 cut(s)	90, 601, 629, 2138, 2670, 3117
TatI	WGTACW	4 cut(s)	183, 862, 2418, 2704
TauI	GCSGC	2 cut(s)	130, 1527
TscAI	CASTG	4 cut(s)	54, 1932, 2267, 2950
TseFI	GTSAC	4 cut(s)	273, 1452, 1553, 1715
TseI	GCWGC	8 cut(s)	361, 482, 759, 1148, 2192, 2522, 3062, 3244
Tsp45I	GTSAC	4 cut(s)	273, 1452, 1553, 1715
TspGWI	ACGGA	3 cut(s)	488, 1233, 2220
TspRI	CASTG	4 cut(s)	54, 1932, 2267, 2950
Van91I	CCANNNNNTGG	4 cut(s)	422, 574, 1831, 2568
Vha464I	CTTAAG	1 cut(s)	973
VpaK11BI	GGWCC	7 cut(s)	430, 656, 1648, 1703, 2216, 2612, 2901
VspI	ATTAAT	1 cut(s)	2763
XagI	CCTNNNNNAGG	1 cut(s)	1050
XapI	RAATTY	5 cut(s)	614, 1615, 2113, 3001, 3281
XbaI	TCTAGA	1 cut(s)	2044
XceI	RCATGY	2 cut(s)	2232, 3206
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2267
XhoI	CTCGAG	1 cut(s)	600
XmnI	GAANNNNTTC	2 cut(s)	96, 2069
XspI	CTAG	6 cut(s)	590, 990, 1283, 2045, 2277, 2435
ZraI	GACGTC	1 cut(s)	1241
Zsp2I	ATGCAT	2 cut(s)	1279, 2547

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.