Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0001577

Physical Location & Seq

Genomic Coordinates Reverse (-)

43524585 .. 43531636

Gene Structure 7052 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00422720.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3300 bp

ATGTCGTCGGAGAAGCTCTTTGGCAAGGGTGCCGTACACTCCGACTCGTTCCGGCAATGCGGGGAGATGGTGCCGATTTTTGAACAGTTGCCGGTGGCCACTATCGTATCCGTGTCGCGCCCCGATACTGGAGATATCAGCCCCATGCTCTTGTCTTACACAATTGAGTTTCAGTACAAACAGCAGTTCAAGTGGCGGTTACTGAAGAAAGCCTCACAAGTTCTCTATCTACATTTTGCATTGAAGAAACGAGCAATAATTGAGGAGTTCCATGAGAAACAAGAGCAGGTTAAAGAATGGCTCCACAGCATAGGAATAGCAGATCAGACAGCAGTAGTGGAAGATGATGATGAACCAGATGATGGGGCTGTTCCTTTACATAATGAAGATAGTGTTAGAAACAGATATGTTCCATCTCGTGCCGCTTTGCCCATCATCCGTCCAGCACTAGGAGGGCAGCAGTCCATCTCAGACCTAGCAAAGGTGGCAATGCAGGGTTATTTGAACCATTTTTTGGGAAACATGGATCTAGTGAATTGTCGAGAGGTCTGCAAATTTTTGGAGGTTTCACAGTTGTCCTTCTTACAAGAGTATGGACCAAAATTGAAAGAAGGTTATGTCATGGTGAAGCATCTGCCAAACTTTTCAGGGGCTAATTCTGATGTGGCATCTTGTGCTGGCTTTTGTCTGGGTTATTGTAGCAACAATTGGCAAAAGGTCTGGGCTGTTTTAAAACCTGGGTTCTTGGCCTTACTGGATGATCCTTTTGACACTGAACTTCTAGACATTATCCTTTTCAATGCTCTTCCACCCTCAAATGGAAATGGAGGATCTGAAAATTATTTGGCAAGTCAAATAAAGGAACGAAACCCTTTACGTTATACATTTAGGGTTGCTTCTGGAAATCAAAGCTTAAGGATAAGAACCACTAGCAATGCTAAAGTTAAAGATTGGGTTACTGCAATTAATGATGCTGGCCTAAGGCCTCATGAAGGTTGGTGCCATCCTCATCGCTTTGGTTCCTTTGCTCCTCCCAGGGGTTTGACTGATGATGGGAGTCAAGCTCAGTGGTTTATAGATGGTAAAGCAGCATTTGAAGCTATTGCTTCCTCTATTGAGGGTGCAAAATCAGAGATATTCATTACTGGCTGGTGGCTTTGCCCAGAACTGTATCTAAGACGTCCTTTCAGTAGCAACTCTTCTTCTAGGCTTGATGCTTTACTAGAAGCAAAAGCTAAGCAAGGGGTTCAGATTTACATTCTTCTCTACAAGGAGGTTTCTCTTGCTTTAAAGATCAACAGCTTATACAGTAAGAAAAAGCTTTCAAATATTCATGAGAATGTGAGAGTAATGCGCTACCCCGACCGTTTTCCCACAGGAATTTACTTATGGTCACACCATGAAAAACTAGTCATTGTTGATTATCAGATTTGCTACATTGGAGGATTAGATTTGTGCTTTGGCCGTTATGACACGGTTGAACACAAAGTGGGTGACTGCCCTCCTTCTGTATGGCCAGGGAAGGACTATTATAACCCAAGAGAATCTGAACCAAATTCTTGGGAAGATGTAATGAAAGACGAATTGGAGCGTGAAAAAGTTCCGCGTATGCCATGGCATGATGTCCACTGTGCTCTCTGGGGACCTCCTTGTCGTGACATTGCAAGGCACTTTGTTCAGCGGTGGAACCATGCTAAGAGAAACAAAGCTCCAAATGAACAAGCACTTCCACTACTTATGCCTCAACACCACATGGTCATCCCTCATTACATGGGAAGAAGTAGAGAGATAGACATTGAGAAAAGGAAAGTGGAAGAGACCCAGAATGGGATCCAGATAGAGAACTCCTTTTCCTCTCTAACACCTTTGGAAAATATCCCGTTGCTTTTGCCTCAAGAAGCTGACGGTTTGGAACCTCCTGGTGTAGATCAAAAGTTAAGTGCCCAGCAGATGCATCATTATCCTGTTGACCAGCCAAATGGGATATCTGGTAGTGTTTCATTATCTTCCAGGAAGCCCAAAGTCAAAGCTTTAGACCCAGATACGCAGATGAATGGTTTGATGGATGATCTTTATTCTATGGATCTTGAAAGTGGAACCAATATATACTCAATGGCCCAATCTGGCTTGACAGCTTCAAATGAATGGTCAGAAAGTTCAGAGGAAAGTGATCATGCTGTTGCTGCTGATGACTGTGGACAAACAGGACCTCGTACTGCATGCAAATGTCAGGTAATTAGAAGTGTGAGCCAGTGGTCTGCTGGAACTAGCCAAACTGAAGAAAGCATACATAATGCTTATTGCTCTCTCATCGAGAAAGCAGAACACTTGGTGTATATTGAGAATCAATTTTTCATATCAGGTCTTTCAGGGGATGAGATTATACAGAACCGTATCCTGGAAGTATTGTACAGGCGTATTGTTCTAGCACACAAAGAACAAAAGTGTTTTAGGGTTATTGTTGTCATGCCACTCTTGCCTGGCTTTCAGGGTGGCGTGGATGATTATGGTGCAGCAACTGTTAGAGCCATTATGCATTGGCAGTATCGAACCATTTGCTGGGAGAAGTATTCAGTACTCCATAAACTTAAAGTTTTGCTTGGTCCTAAGACACATGACTACATTTCTTTCTATGGTCTGAGAACATATGGTAAACTGTTTGAAGATGGCCCTCTATCCACAAGTCAGGTATATGTACACAGTAAAGTGATGATTGTAGATGACCGTGTTGCTTTGATTGGATCATCAAATATTAATGACAGAAGTTTACTTGGATCAAGAGACTCTGAGATTGGTGTAGTTATAGAAGATAAAGAATTTCTTGAATCGTCCATGGCCGGACAGCCTTGGAAGGCCGGAAAGTTTTCTTATAGTCTTAGGTGCTCCCTGTGGTCTGAACACCTGGGTCTTCATGCAGGAGAGATAAATCAAATCAGCGATCCTGTGTCAGACTCAGCATACAAAGATCTGTGGCTAGCGACTGCAAAGGAAAATTCAATTATTTACCAAGATGTATTTTCTTGTATTCCGAATGATTCTATAAATTCAAGAGCTGCTCTCAGAGAATCCATCGCTTTTTGGAAAGAGAAGCTCGGTCACACTACAATCGATCTGGGAATAGCTCCTAAGAAGCTAGAATCCTGGGAGAATGGAGAGATTAAAGAAACAGATCCAATGGAAAGATTGAAACATATAAGGGGACATCTTGTTTCCTTTCCTTTAGAGTTTATGAACCAAGAAGATCTGAGACCAGTGTTTAATGAAAGTGAATTTTATACATCTCCTCAAGTATTTATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004620 GO:0004630 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0006629 GO:0006643 GO:0006644 GO:0006664 GO:0006793 GO:0006796 GO:0006950 GO:0006995 GO:0007154 GO:0007275 GO:0008081 GO:0008150 GO:0008152 GO:0008610 GO:0009056 GO:0009058 GO:0009247 GO:0009267 GO:0009395 GO:0009605 GO:0009719 GO:0009725 GO:0009733 GO:0009987 GO:0009991 GO:0010033 GO:0016036 GO:0016042 GO:0016298 GO:0016787 GO:0016788 GO:0019374 GO:0019375 GO:0019637 GO:0022622 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0032879 GO:0033554 GO:0042221 GO:0042578 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043562 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0046434 GO:0046467 GO:0048364 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051716 GO:0060627 GO:0065007 GO:0071496 GO:0071704 GO:0099402 GO:1901135 GO:1901137 GO:1901575 GO:1901576 GO:1903509

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

PH PLDc PLDc_2

Protein Analysis

1099

Amino Acids

124.36

Weight (kDa)

5.87

Isoelectric Point (pI)

44.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PLDc	PF00614	462 - 489	6.2e-07	Phospholipase D Active site motif
PLDc_2	PF13091	864 - 942	6.2e-09	PLD-like domain
PLDc	PF00614	899 - 922	1.4e-07	Phospholipase D Active site motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0001577)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16785 AT3G16785 AT3G16785 AT3G16785 AT3G16785 AT3G16785
fragaria_vesca	FvH4_4g18810 FvH4_6g06940 FvH4_6g06940 FvH4_6g06940
malus_domestica	MD04G1197500.v1.1 MD12G1210300.v1.1 MD13G1162600.v1.1 MD16G1162400.v1.1
prunus_persica	Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.6G317900_v2.0.a1 Prupe.6G317900_v2.0.a1 Prupe.6G317900_v2.0.a1
pyrus_communis	pycom04g17480 pycom13g13890 pycom16g13770
rosa_chinensis	RchiOBHm_Chr3g0456481 RchiOBHm_Chr4g0423621
rosa_laevigata	RLG00000007541 RLG00000025302
rosa_multiflora	Rmu_co8413375.1_g000001 Rmu_sc0000464.1_g000017 Rmu_sc0002607.1_g000011 Rmu_sc0008373.1_g000012 Rmu_sc0016309.1_g000001
rosa_roxburghii	Rroxscaffold_5G00365830 Rroxscaffold_6G00422720
rosa_rugosa	Rorug03G0013400 Rorug03G0013400 Rorug03G0013400 Rorug04G0189100
rosa_samantha	Rh3AG073700 Rh3BG075600 Rh3DG076900 Rh4AG248700 Rh4BG254000 Rh4DG249000
rosa_wichuraiana	Rw3G006320 Rw4G021300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1533
AasI	GACNNNNNNGTC	1 cut(s)	1650
AatII	GACGTC	1 cut(s)	1183
Acc36I	ACCTGC	1 cut(s)	277
AccB1I	GGYRCC	3 cut(s)	29, 70, 999
AccB7I	CCANNNNNTGG	3 cut(s)	362, 514, 2559
AccII	CGCG	2 cut(s)	118, 1606
AciI	CCGC	5 cut(s)	60, 196, 423, 1604, 1681
AcoI	YGGCCR	4 cut(s)	96, 1462, 1514, 2835
AcsI	RAATTY	7 cut(s)	554, 1380, 1555, 2816, 2992, 3043, 3269
AcuI	CTGAAG	2 cut(s)	224, 2298
AcyI	GRCGYC	1 cut(s)	1180
AdeI	CACNNNGTG	2 cut(s)	1489, 2332
AfaI	GTAC	6 cut(s)	36, 176, 2215, 2411, 2577, 2697
AflII	CTTAAG	1 cut(s)	913
AhlI	ACTAGT	1 cut(s)	1408
AjnI	CCWGG	9 cut(s)	736, 1034, 1516, 1918, 2009, 2397, 2479, 2901, 3140
AjuI	GAANNNNNNNTTGG	4 cut(s)	497, 529, 1568, 1600
AloI	GAACNNNNNNTCC	2 cut(s)	1835, 1867
Alw21I	GWGCWC	2 cut(s)	1636, 2885
Alw26I	GTCTC	3 cut(s)	1811, 2775, 3241
AlwNI	CAGNNNCTG	1 cut(s)	2519
ApeKI	GCWGC	5 cut(s)	457, 1088, 2183, 2513, 3053
ApoI	RAATTY	7 cut(s)	554, 1380, 1555, 2816, 2992, 3043, 3269
ArsI	GACNNNNNNTTYG	2 cut(s)	2132, 2164
AseI	ATTAAT	2 cut(s)	966, 2754
Asp700I	GAANNNNTTC	2 cut(s)	1599, 2863
AspLEI	GCGC	2 cut(s)	120, 1356
AspS9I	GGNCC	6 cut(s)	596, 1643, 2116, 2207, 2603, 2669
AsuHPI	GGTGA	2 cut(s)	637, 1505
AsuNHI	GCTAGC	1 cut(s)	2974
AvaII	GGWCC	4 cut(s)	596, 1643, 2207, 2603
AxyI	CCTNAGG	1 cut(s)	980
BaeGI	GKGCMC	1 cut(s)	1945
BaeI	ACNNNNGTAYC	2 cut(s)	90, 123
BalI	TGGCCA	2 cut(s)	98, 1516
BamHI	GGATCC	1 cut(s)	1830
BanI	GGYRCC	3 cut(s)	29, 70, 999
BarI	GAAGNNNNNNTAC	4 cut(s)	1183, 1215, 2271, 2303
BauI	CACGAG	1 cut(s)	417
BbsI	GAAGAC	1 cut(s)	2900
Bbv12I	GWGCWC	2 cut(s)	1636, 2885
BbvI	GCAGC	5 cut(s)	469, 1100, 2170, 2525, 3040
BceAI	ACGGC	2 cut(s)	17, 1449
BcgI	CGANNNNNNTGC	4 cut(s)	531, 565, 2538, 2572
BciT130I	CCWGG	9 cut(s)	738, 1036, 1518, 1920, 2011, 2399, 2481, 2903, 3142
BciVI	GTATCC	2 cut(s)	118, 2405
BclI	TGATCA	1 cut(s)	2170
BcoDI	GTCTC	3 cut(s)	1811, 2775, 3241
BcuI	ACTAGT	1 cut(s)	1408
BfrI	CTTAAG	1 cut(s)	913
BfuAI	ACCTGC	1 cut(s)	277
BfuI	GTATCC	2 cut(s)	118, 2405
BglII	AGATCT	2 cut(s)	2965, 3241
BisI	GCNGC	6 cut(s)	423, 458, 1089, 2184, 2514, 3054
BlpI	GCTNAGC	1 cut(s)	1236
BlsI	GCNGC	6 cut(s)	424, 459, 1090, 2185, 2515, 3055
BmcAI	AGTACT	1 cut(s)	2577
Bme1390I	CCNGG	9 cut(s)	738, 1036, 1518, 1920, 2011, 2399, 2481, 2903, 3142
Bme18I	GGWCC	4 cut(s)	596, 1643, 2207, 2603
BmgT120I	GGNCC	6 cut(s)	596, 1643, 2116, 2207, 2603, 2669
BmrFI	CCNGG	9 cut(s)	738, 1036, 1518, 1920, 2011, 2399, 2481, 2903, 3142
BmsI	GCATC	6 cut(s)	640, 677, 961, 1204, 1941, 1963
BmtI	GCTAGC	1 cut(s)	2978
BpiI	GAAGAC	1 cut(s)	2900
BplI	GAGNNNNNCTC	4 cut(s)	1048, 1080, 3042, 3074
BpmI	CTGGAG	1 cut(s)	150
Bpu1102I	GCTNAGC	1 cut(s)	1236
BpuEI	CTTGAG	2 cut(s)	1878, 3270
Bsa29I	ATCGAT	1 cut(s)	3108
BsaHI	GRCGYC	1 cut(s)	1180
BsaI	GGTCTC	2 cut(s)	1811, 3241
BsaJI	CCNNGG	9 cut(s)	737, 1034, 1035, 1517, 1613, 2832, 2846, 2902, 3141
Bse118I	RCCGGY	1 cut(s)	91
Bse1I	ACTGG	5 cut(s)	133, 759, 1150, 2251, 3251
Bse21I	CCTNAGG	1 cut(s)	980
Bse3DI	GCAATG	4 cut(s)	62, 495, 940, 1659
BseBI	CCWGG	9 cut(s)	738, 1036, 1518, 1920, 2011, 2399, 2481, 2903, 3142
BseCI	ATCGAT	1 cut(s)	3108
BseDI	CCNNGG	9 cut(s)	737, 1034, 1035, 1517, 1613, 2832, 2846, 2902, 3141
BseGI	GGATG	7 cut(s)	435, 763, 1003, 1758, 2071, 2380, 2506
BseMI	GCAATG	4 cut(s)	62, 495, 940, 1659
BseMII	CTCAG	7 cut(s)	483, 1079, 2630, 2778, 2967, 3073, 3236
BseNI	ACTGG	5 cut(s)	133, 759, 1150, 2251, 3251
BseRI	GAGGAG	3 cut(s)	278, 1020, 3273
BseSI	GKGCMC	1 cut(s)	1945
BseXI	GCAGC	5 cut(s)	469, 1100, 2170, 2525, 3040
BseYI	CCCAGC	2 cut(s)	1944, 2559
BsgI	GTGCAG	1 cut(s)	2532
Bsh1236I	CGCG	2 cut(s)	118, 1606
Bsh1285I	CGRYCG	1 cut(s)	1366
BshNI	GGYRCC	3 cut(s)	29, 70, 999
BshVI	ATCGAT	1 cut(s)	3108
BsiEI	CGRYCG	1 cut(s)	1366
BsiHKAI	GWGCWC	2 cut(s)	1636, 2885
BsiSI	CCGG	4 cut(s)	52, 92, 2838, 2856
BslFI	GGGAC	2 cut(s)	1656, 3213
BsmAI	GTCTC	3 cut(s)	1811, 2775, 3241
BsmFI	GGGAC	2 cut(s)	1656, 3213
Bso31I	GGTCTC	2 cut(s)	1811, 3241
Bsp1286I	GDGCHC	3 cut(s)	1636, 1945, 2885
Bsp1407I	TGTACA	2 cut(s)	2409, 2695
Bsp1720I	GCTNAGC	1 cut(s)	1236
Bsp19I	CCATGG	2 cut(s)	1613, 2832
BspACI	CCGC	5 cut(s)	60, 196, 423, 1604, 1681
BspCNI	CTCAG	7 cut(s)	482, 1078, 2631, 2779, 2966, 3072, 3237
BspDI	ATCGAT	1 cut(s)	3108
BspFNI	CGCG	2 cut(s)	118, 1606
BspHI	TCATGA	2 cut(s)	988, 1333
BspMI	ACCTGC	1 cut(s)	277
BspOI	GCTAGC	1 cut(s)	2978
BspQI	GCTCTTC	1 cut(s)	810
BspT107I	GGYRCC	3 cut(s)	29, 70, 999
BspTI	CTTAAG	1 cut(s)	913
BspTNI	GGTCTC	2 cut(s)	1811, 3241
BsrDI	GCAATG	4 cut(s)	62, 495, 940, 1659
BsrFI	RCCGGY	1 cut(s)	91
BsrGI	TGTACA	2 cut(s)	2409, 2695
BsrI	ACTGG	5 cut(s)	133, 759, 1150, 2251, 3251
BssAI	RCCGGY	1 cut(s)	91
BssECI	CCNNGG	9 cut(s)	737, 1034, 1035, 1517, 1613, 2832, 2846, 2902, 3141
BssNI	GRCGYC	1 cut(s)	1180
BssSI	CACGAG	1 cut(s)	417
BssT1I	CCWWGG	3 cut(s)	1613, 2832, 2846
Bst2BI	CACGAG	1 cut(s)	417
Bst2UI	CCWGG	9 cut(s)	738, 1036, 1518, 1920, 2011, 2399, 2481, 2903, 3142
Bst6I	CTCTTC	3 cut(s)	810, 1204, 1809
BstACI	GRCGYC	1 cut(s)	1180
BstAFI	CTTAAG	1 cut(s)	913
BstAPI	GCANNNNNTGC	1 cut(s)	674
BstAUI	TGTACA	2 cut(s)	2409, 2695
BstC8I	GCNNGC	4 cut(s)	679, 976, 2221, 2976
BstDSI	CCRYGG	2 cut(s)	1613, 2832
BstENI	CCTNNNNNAGG	2 cut(s)	479, 990
BstF5I	GGATG	7 cut(s)	435, 763, 1003, 1758, 2071, 2380, 2506
BstFNI	CGCG	2 cut(s)	118, 1606
BstHHI	GCGC	2 cut(s)	120, 1356
BstMAI	GTCTC	3 cut(s)	1811, 2775, 3241
BstMCI	CGRYCG	1 cut(s)	1366
BstMWI	GCNNNNNNNGC	5 cut(s)	485, 674, 1097, 2183, 2476
BstNI	CCWGG	9 cut(s)	738, 1036, 1518, 1920, 2011, 2399, 2481, 2903, 3142
BstNSI	RCATGY	1 cut(s)	2223
BstSCI	CCNGG	9 cut(s)	736, 1034, 1516, 1918, 2009, 2397, 2479, 2901, 3140
BstSLI	GKGCMC	1 cut(s)	1945
BstUI	CGCG	2 cut(s)	118, 1606
BstV1I	GCAGC	5 cut(s)	469, 1100, 2170, 2525, 3040
BstV2I	GAAGAC	1 cut(s)	2900
BstX2I	RGATCY	7 cut(s)	526, 830, 1830, 2083, 2965, 3169, 3241
BstXI	CCANNNNNNTGG	2 cut(s)	1560, 1979
BstYI	RGATCY	7 cut(s)	526, 830, 1830, 2083, 2965, 3169, 3241
Bsu15I	ATCGAT	1 cut(s)	3108
Bsu36I	CCTNAGG	1 cut(s)	980
BsuI	GTATCC	2 cut(s)	118, 2405
BsuTUI	ATCGAT	1 cut(s)	3108
BtgI	CCRYGG	2 cut(s)	1613, 2832
BtgZI	GCGATG	2 cut(s)	995, 3055
BtsCI	GGATG	7 cut(s)	435, 763, 1003, 1758, 2071, 2380, 2506
BtsIMutI	CAGTG	5 cut(s)	771, 1073, 1627, 2258, 3258
BveI	ACCTGC	1 cut(s)	277
Cac8I	GCNNGC	4 cut(s)	679, 976, 2221, 2976
CaiI	CAGNNNCTG	1 cut(s)	2519
CciI	TCATGA	2 cut(s)	988, 1333
CfoI	GCGC	2 cut(s)	120, 1356
Cfr10I	RCCGGY	1 cut(s)	91
Cfr13I	GGNCC	6 cut(s)	596, 1643, 2116, 2207, 2603, 2669
ClaI	ATCGAT	1 cut(s)	3108
Csp6I	GTAC	6 cut(s)	35, 175, 2214, 2410, 2576, 2696
CspCI	CAANNNNNGTGG	2 cut(s)	1049, 1084
CviQI	GTAC	6 cut(s)	35, 175, 2214, 2410, 2576, 2696
DraI	TTTAAA	2 cut(s)	732, 1290
DraIII	CACNNNGTG	2 cut(s)	1489, 2332
DrdI	GACNNNNNNGTC	1 cut(s)	1650
DseDI	GACNNNNNNGTC	1 cut(s)	1650
EaeI	YGGCCR	4 cut(s)	96, 1462, 1514, 2835
Eam1104I	CTCTTC	3 cut(s)	810, 1204, 1809
EarI	CTCTTC	3 cut(s)	810, 1204, 1809
Eco130I	CCWWGG	3 cut(s)	1613, 2832, 2846
Eco147I	AGGCCT	1 cut(s)	985
Eco31I	GGTCTC	2 cut(s)	1811, 3241
Eco32I	GATATC	2 cut(s)	136, 1986
Eco47I	GGWCC	4 cut(s)	596, 1643, 2207, 2603
Eco57I	CTGAAG	2 cut(s)	224, 2298
Eco81I	CCTNAGG	1 cut(s)	980
EcoNI	CCTNNNNNAGG	2 cut(s)	479, 990
EcoO109I	RGGNCCY	2 cut(s)	1643, 2207
EcoRII	CCWGG	9 cut(s)	736, 1034, 1516, 1918, 2009, 2397, 2479, 2901, 3140
EcoRV	GATATC	2 cut(s)	136, 1986
EcoT14I	CCWWGG	3 cut(s)	1613, 2832, 2846
EcoT22I	ATGCAT	2 cut(s)	1956, 2538
ErhI	CCWWGG	3 cut(s)	1613, 2832, 2846
FalI	AAGNNNNNCTT	6 cut(s)	1168, 1200, 2583, 2615, 2805, 2837
FaqI	GGGAC	2 cut(s)	1656, 3213
FauI	CCCGC	1 cut(s)	53
FauNDI	CATATG	1 cut(s)	2647
FbaI	TGATCA	1 cut(s)	2170
Fnu4HI	GCNGC	6 cut(s)	423, 458, 1089, 2184, 2514, 3054
FokI	GGATG	7 cut(s)	422, 770, 990, 1745, 2078, 2387, 2513
Fsp4HI	GCNGC	6 cut(s)	423, 458, 1089, 2184, 2514, 3054
GlaI	GCGC	2 cut(s)	119, 1355
GluI	GCNGC	6 cut(s)	423, 458, 1089, 2184, 2514, 3054
GsaI	CCCAGC	2 cut(s)	1948, 2563
GsuI	CTGGAG	1 cut(s)	150
HapII	CCGG	4 cut(s)	52, 92, 2838, 2856
HhaI	GCGC	2 cut(s)	120, 1356
Hin1I	GRCGYC	1 cut(s)	1180
Hin6I	GCGC	2 cut(s)	118, 1354
HinP1I	GCGC	2 cut(s)	118, 1354
HincII	GTYRAC	1 cut(s)	1969
HindII	GTYRAC	1 cut(s)	1969
HindIII	AAGCTT	3 cut(s)	910, 1319, 2028
HpaII	CCGG	4 cut(s)	52, 92, 2838, 2856
HphI	GGTGA	2 cut(s)	637, 1505
Hpy166II	GTNNAC	7 cut(s)	37, 1627, 1969, 2198, 2654, 2698, 2768
Hpy8I	GTNNAC	7 cut(s)	37, 1627, 1969, 2198, 2654, 2698, 2768
Hpy99I	CGWCG	1 cut(s)	10
HpyAV	CCTTC	6 cut(s)	589, 605, 986, 1515, 1516, 2845
HpyCH4IV	ACGT	2 cut(s)	877, 1180
HpyF10VI	GCNNNNNNNGC	5 cut(s)	485, 674, 1097, 2183, 2476
HpySE526I	ACGT	2 cut(s)	877, 1180
Hsp92I	GRCGYC	1 cut(s)	1180
HspAI	GCGC	2 cut(s)	118, 1354
Ksp22I	TGATCA	1 cut(s)	2170
LguI	GCTCTTC	1 cut(s)	810
LmnI	GCTCC	6 cut(s)	306, 1033, 1588, 1714, 2888, 3127
Lsp1109I	GCAGC	5 cut(s)	469, 1100, 2170, 2525, 3040
LweI	GCATC	6 cut(s)	640, 677, 961, 1204, 1941, 1963
MaeII	ACGT	2 cut(s)	877, 1180
MaeIII	GTNAC	6 cut(s)	198, 955, 1392, 1493, 1655, 3095
MfeI	CAATTG	2 cut(s)	162, 706
MflI	RGATCY	7 cut(s)	526, 830, 1830, 2083, 2965, 3169, 3241
MhlI	GDGCHC	3 cut(s)	1636, 1945, 2885
MlsI	TGGCCA	2 cut(s)	98, 1516
MluNI	TGGCCA	2 cut(s)	98, 1516
MlyI	GAGTC	4 cut(s)	38, 1066, 2777, 2945
MmeI	TCCRAC	1 cut(s)	66
Mox20I	TGGCCA	2 cut(s)	98, 1516
Mph1103I	ATGCAT	2 cut(s)	1956, 2538
MroXI	GAANNNNTTC	2 cut(s)	1599, 2863
MscI	TGGCCA	2 cut(s)	98, 1516
MslI	CAYNNNNRTG	2 cut(s)	1338, 2224
Msp20I	TGGCCA	2 cut(s)	98, 1516
MspA1I	CMGCKG	1 cut(s)	1681
MspCI	CTTAAG	1 cut(s)	913
MspI	CCGG	4 cut(s)	52, 92, 2838, 2856
MspR9I	CCNGG	9 cut(s)	738, 1036, 1518, 1920, 2011, 2399, 2481, 2903, 3142
MunI	CAATTG	2 cut(s)	162, 706
MvaI	CCWGG	9 cut(s)	738, 1036, 1518, 1920, 2011, 2399, 2481, 2903, 3142
MvnI	CGCG	2 cut(s)	118, 1606
MwoI	GCNNNNNNNGC	5 cut(s)	485, 674, 1097, 2183, 2476
NcoI	CCATGG	2 cut(s)	1613, 2832
NdeI	CATATG	1 cut(s)	2647
NheI	GCTAGC	1 cut(s)	2974
NmuCI	GTSAC	4 cut(s)	1392, 1493, 1655, 3095
NsiI	ATGCAT	2 cut(s)	1956, 2538
NspI	RCATGY	1 cut(s)	2223
PaeI	GCATGC	1 cut(s)	2223
PagI	TCATGA	2 cut(s)	988, 1333
PasI	CCCWGGG	1 cut(s)	1035
PceI	AGGCCT	1 cut(s)	985
PciSI	GCTCTTC	1 cut(s)	810
PdmI	GAANNNNTTC	2 cut(s)	1599, 2863
PfeI	GAWTC	6 cut(s)	1544, 2344, 2825, 3035, 3065, 3137
PflMI	CCANNNNNTGG	3 cut(s)	362, 514, 2559
PfoI	TCCNGGA	2 cut(s)	2009, 2397
PkrI	GCNGC	6 cut(s)	424, 459, 1090, 2185, 2515, 3055
PleI	GAGTC	4 cut(s)	38, 1065, 2777, 2945
PpsI	GAGTC	4 cut(s)	38, 1065, 2777, 2945
PpuMI	RGGWCCY	2 cut(s)	1643, 2207
PshBI	ATTAAT	2 cut(s)	966, 2754
PsiI	TTATAA	1 cut(s)	1533
Psp5II	RGGWCCY	2 cut(s)	1643, 2207
Psp6I	CCWGG	9 cut(s)	736, 1034, 1516, 1918, 2009, 2397, 2479, 2901, 3140
PspFI	CCCAGC	2 cut(s)	1944, 2559
PspGI	CCWGG	9 cut(s)	736, 1034, 1516, 1918, 2009, 2397, 2479, 2901, 3140
PspPI	GGNCC	6 cut(s)	596, 1643, 2116, 2207, 2603, 2669
PspPPI	RGGWCCY	2 cut(s)	1643, 2207
PstNI	CAGNNNCTG	1 cut(s)	2519
PsuI	RGATCY	7 cut(s)	526, 830, 1830, 2083, 2965, 3169, 3241
RsaI	GTAC	6 cut(s)	36, 176, 2215, 2411, 2577, 2697
RsaNI	GTAC	6 cut(s)	35, 175, 2214, 2410, 2576, 2696
RseI	CAYNNNNRTG	2 cut(s)	1338, 2224
SapI	GCTCTTC	1 cut(s)	810
SatI	GCNGC	6 cut(s)	423, 458, 1089, 2184, 2514, 3054
Sau96I	GGNCC	6 cut(s)	596, 1643, 2116, 2207, 2603, 2669
ScaI	AGTACT	1 cut(s)	2577
SchI	GAGTC	4 cut(s)	38, 1066, 2777, 2945
ScrFI	CCNGG	9 cut(s)	738, 1036, 1518, 1920, 2011, 2399, 2481, 2903, 3142
SduI	GDGCHC	3 cut(s)	1636, 1945, 2885
SfaNI	GCATC	6 cut(s)	640, 677, 961, 1204, 1941, 1963
SinI	GGWCC	4 cut(s)	596, 1643, 2207, 2603
SmiMI	CAYNNNNRTG	2 cut(s)	1338, 2224
SmlI	CTYRAG	3 cut(s)	913, 1893, 3285
SmoI	CTYRAG	3 cut(s)	913, 1893, 3285
SpeI	ACTAGT	1 cut(s)	1408
SphI	GCATGC	1 cut(s)	2223
SseBI	AGGCCT	1 cut(s)	985
SsiI	CCGC	5 cut(s)	60, 196, 423, 1604, 1681
SspI	AATATT	2 cut(s)	1330, 2752
StuI	AGGCCT	1 cut(s)	985
StyD4I	CCNGG	9 cut(s)	736, 1034, 1516, 1918, 2009, 2397, 2479, 2901, 3140
StyI	CCWWGG	3 cut(s)	1613, 2832, 2846
TaiI	ACGT	2 cut(s)	880, 1183
TaqI	TCGA	4 cut(s)	541, 2313, 2548, 3108
TaqII	GACCGA	1 cut(s)	3083
TatI	WGTACW	4 cut(s)	174, 2409, 2575, 2695
TauI	GCSGC	1 cut(s)	425
TfiI	GAWTC	6 cut(s)	1544, 2344, 2825, 3035, 3065, 3137
TscAI	CASTG	5 cut(s)	778, 1073, 1634, 2258, 3258
TseFI	GTSAC	4 cut(s)	1392, 1493, 1655, 3095
TseI	GCWGC	5 cut(s)	457, 1088, 2183, 2513, 3053
Tsp45I	GTSAC	4 cut(s)	1392, 1493, 1655, 3095
TspGWI	ACGGA	2 cut(s)	100, 428
TspRI	CASTG	5 cut(s)	778, 1073, 1634, 2258, 3258
Van91I	CCANNNNNTGG	3 cut(s)	362, 514, 2559
Vha464I	CTTAAG	1 cut(s)	913
VpaK11BI	GGWCC	4 cut(s)	596, 1643, 2207, 2603
VspI	ATTAAT	2 cut(s)	966, 2754
XagI	CCTNNNNNAGG	2 cut(s)	479, 990
XapI	RAATTY	7 cut(s)	554, 1380, 1555, 2816, 2992, 3043, 3269
XbaI	TCTAGA	1 cut(s)	781
XceI	RCATGY	1 cut(s)	2223
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2258
XmnI	GAANNNNTTC	2 cut(s)	1599, 2863
ZraI	GACGTC	1 cut(s)	1181
ZrmI	AGTACT	1 cut(s)	2577
Zsp2I	ATGCAT	2 cut(s)	1956, 2538

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.