Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0001577

Physical Location & Seq

Genomic Coordinates Forward (+)

47557605 .. 47566643

Gene Structure 9039 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00365830.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3036 bp

ATGGAATCGGAGCAGTTGATATCATCGACGGGGAGCGGTTCCCGGTATATGCAAATGCGATCGGAGCAGTTAACGTCGCCGTCGTCGCTGTTATCGTTCCGGCACTCGTCCTTCGAGCCCCCGCGGATTTTCGACGAGTTGCCCAGCGCCACCATCGTCTCCGTCTCCCGCCCCGACGCCGGCGATATCAGCCCCATGCTTCTCTCCTACACTATCGAGTTCCAATACAAGCAGGCACGATCACCATCTCTCTTTTTCTCTCGCTTTCTGTATTTGCTTCTTCTTTCTTCTTTCTCTATGGACGAGATCGCTTTGCATGACATGCTTGGTTATAGTTCCATCGTGCTAGGCTTCGATCAAGATGTTCCATCTAGTGCCGCTCTACCAATCATTCGGCCAGCCCCTGGTGGTAGGCAGCAATCCATTTCGGACAGATCAAAGATTGCAATGCAAGGATATCTTAATCACTTTCTTGGAAACATGGATATTGTGAACTCTCAAGAGGTCTGCAAGTTTTTGGAGGTCTCTAAGCTATCATTTTCTCCAGAATATGGCCCTAAGCTGAAAGAAGATTTTGTAATGGTGAAACATCTACCAAAATTGCCAAAGAATGATCCTTCCAGGAAATGTTGCTCATGTCGTTGGTTCAGTTGTTGTAATGACAACTGGCAAAAGGTTTGGGCTGTATTAAAACCAGGATTCTTGGCCTTTCTGGCAGATCCTTTTGATACTCAACCTTTAGACATAATAGTATTTGATGTACTACCAGCCTCGGATGGTAATGGGGATGGCCGTGTATCACTAGCGAAAGAGATAAAAGATAGAAATCCACTACGCCATGCATTTCAGGTTGGTGTCATCCTCACCGCTTTGGCTCCTTTGCGCCTCCCAAGAGGCTTGACTGAAGATGGTAGTCAGGCTCAATGGTTTGTAGATGGTCAGGCGGCATTTGAAGCTATTGCTTCTGCAATTGAGGATGCAAAATCAGAGATATTTATTTGTGGTTGGTGGCTGTGTCCAGAATTGTACATGCGGCGTCCGTTTCTTACTCATGCTTCTTCTAAACTTGATTCTCTGCTGGAAGCAAAAGCTAAGGAAGGGGTTCAGATTTACATTCTTCTCTATAAAGAGGTAGCTCTTGCTCTGAAAATCAACAGCGTTTATAGCAAGAGAAAACTTCTTGGGATTCATGAAAATGTGAGAGTATTACGTTATCCTGACCACTTCTCTAGTGGTGTTTACTTATGGTCCCACCATGAAAAACTTGTCATTGTTGATCATCAGATTTGCTTTGTTGGAGGACTTGATTTATGCTTTGGTCGTTATGATACTGCTGAACATAAAGTGGGTGACTGCCCTCCTAACATTTGGCCTGGAAAGGACTATTACAACCCCAGGGAATCTGAACCAAACTCGTGGGAGGATACAATGAGAGATGAGTTGGACCGTGAAAAATATCCTCGCATGCCTTGGCATGATGTCCACTGTGCTATTTGGGGACCTCCTTGCCGTGATGTAGCTAGGCATTTCGTTCAGCGGTGGAACTATGCAAAGAGAAATAAAGCTCCAAACGAGCAGGCAATTCCACTACTTATGCCGCAACAGCACATGGTAATTCCTCATTACAGGGGGAAAAGCTCAAATGTGGAGATTGAAAATGAGAATGCCAGCAATAGTAAAGACATTAAAAGGCAGGATTCCTTTTCGTCGAAATCATCCTATCAGGATATCCCCTTGCTTATACCTCAAGAGGCTGATGGGTTGAATGAAAACCCAAGACCATATGGGGTGGACTCTCCACATTACCTCGATCAGCCAAGCAGAGTTGGAAACAATCTTGCTTTCCCTTTTCGGAAGACAAAAATTGAACCAGTAGACGTGGTGGCACATCCTGCAATCACAAATTCAGATCCAGAGTGGTGGGAAATACAAGAACGGGGAAATAAGGGTGGTTTCACAGATGAATCAGGACAAGTTGGTCCTTCTAGTTCATGCCGTTGTCAGGTTATCAGAAGTGTCAGTCAGTGGTCAGCAGGAACAAGCCAAGTTGAAGATAGCATTCACAGTGCTTATTGTTCTCTTATCGATAAAGCTGAGCACTTTATTTACATTGAGAATCAATTTTTCATCTCAGGTCTTTCAGGAGATGAAATAATACGGAATCGTGTGTTAGAAGCATTATTCTGTCGTATTATGCGAGCATACAATGATAAGAAGTGTTTTAGAGTTATAATTGTCATACCACTCGTACCCGGTTTTCAAGGTGGTTTGGATGATGCTGGCGCGGCATCTGTGAGAGCTGTTATGCATTGGCAATATCGAACCATTTGCAGAGGGCATAATTCAATTCTACACAATTTATATGAACTTCTTGGTCCAAAGACACACGACTACATTTCTTTCTATGGCCTTAGAGCCTATGGTAAACTCTTTGATGGCGGTCCTGTGGCCTCCAGTCAGGTGTATGTGCATAGTAAAATCATGATTGTCGATGATTGTACAACTATAATTGGATCAGCTAACATCAATGATAGGAGTTTGCTTGGTTCAAGAGATTCTGAGATTGGTCTTCTTATTGAAGACAAAGAGTTAGTTAATTCATATATGGGAGGAAAACCATGGAAGGCTGGAAAATTTTCATTGAGTCTTCGCATGTCGCTATGGTCTGAACATCTTGGTATTCGCGCTGGAGAGATGGACCAAATAATCGATCCAACTGTTGATTCAACTTACAAGGATATATGGATGGCAACAGCGAAGACAAATACCACCATCTACCAAGATGTCTTTTCCTGTGTACCAAATGATTTTATACATTCTAGAGCTGCATTTAGACAACATATAGCCTTTTGGAAGGAGAAGGTAGGGCACACAACCATTGATTTAGGAATTGCTCCTGGAACGCTAGAGTCGTATCAAAATGGAGATGTCAAGAAAGCAGATCCCATGGAGAGGTTAGAGTCTATAAAGGGGCATCTTGTTTCTTTCCCTTTTGGATTTCATGTTGAAAGAAGATTTGAGACCTGTGTTCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004620 GO:0004630 GO:0006629 GO:0007275 GO:0008081 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0016298 GO:0016787 GO:0016788 GO:0022622 GO:0032501 GO:0032502 GO:0042578 GO:0044238 GO:0048364 GO:0048731 GO:0048856 GO:0071704 GO:0099402 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

PLDc PLDc_2

Protein Analysis

1011

Amino Acids

114.28

Weight (kDa)

6.26

Isoelectric Point (pI)

45.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PLDc	PF00614	414 - 441	8.7e-08	Phospholipase D Active site motif
PLDc_2	PF13091	805 - 868	4.4e-09	PLD-like domain
PLDc	PF00614	823 - 846	2.1e-06	Phospholipase D Active site motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001577)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16785 AT3G16785 AT3G16785 AT3G16785 AT3G16785 AT3G16785
fragaria_vesca	FvH4_4g18810 FvH4_6g06940 FvH4_6g06940 FvH4_6g06940
malus_domestica	MD04G1197500.v1.1 MD12G1210300.v1.1 MD13G1162600.v1.1 MD16G1162400.v1.1
prunus_persica	Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.6G317900_v2.0.a1 Prupe.6G317900_v2.0.a1 Prupe.6G317900_v2.0.a1
pyrus_communis	pycom04g17480 pycom13g13890 pycom16g13770
rosa_chinensis	RchiOBHm_Chr3g0456481 RchiOBHm_Chr4g0423621
rosa_laevigata	RLG00000007541 RLG00000025302
rosa_multiflora	Rmu_co8413375.1_g000001 Rmu_sc0000464.1_g000017 Rmu_sc0002607.1_g000011 Rmu_sc0008373.1_g000012 Rmu_sc0016309.1_g000001
rosa_roxburghii	Rroxscaffold_5G00365830 Rroxscaffold_6G00422720
rosa_rugosa	Rorug03G0013400 Rorug03G0013400 Rorug03G0013400 Rorug04G0189100
rosa_samantha	Rh3AG073700 Rh3BG075600 Rh3DG076900 Rh4AG248700 Rh4BG254000 Rh4DG249000
rosa_wichuraiana	Rw3G006320 Rw4G021300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2231
AasI	GACNNNNNNGTC	1 cut(s)	1977
AccB7I	CCANNNNNTGG	2 cut(s)	404, 551
AccBSI	CCGCTC	2 cut(s)	36, 380
AccI	GTMKAC	1 cut(s)	1875
AccII	CGCG	3 cut(s)	124, 2285, 2685
AclWI	GGATC	6 cut(s)	608, 713, 1904, 2521, 2705, 2936
AcoI	YGGCCR	2 cut(s)	395, 790
AcsI	RAATTY	2 cut(s)	1903, 2633
AcuI	CTGAAG	1 cut(s)	924
AcyI	GRCGYC	2 cut(s)	177, 1036
AfaI	GTAC	5 cut(s)	762, 1028, 2250, 2500, 2799
AfiI	CCNNNNNNNGG	5 cut(s)	179, 404, 551, 2002, 2952
AjiI	CACGTC	1 cut(s)	1879
AjnI	CCWGG	6 cut(s)	403, 620, 694, 1372, 1394, 2896
AjuI	GAANNNNNNNTTGG	2 cut(s)	2707, 2739
Alw21I	GWGCWC	1 cut(s)	2100
Alw26I	GTCTC	4 cut(s)	163, 169, 529, 3014
AlwI	GGATC	6 cut(s)	608, 713, 1904, 2521, 2705, 2936
AlwNI	CAGNNNCTG	1 cut(s)	404
AoxI	GGCC	7 cut(s)	395, 553, 705, 790, 1370, 2407, 2448
ApeKI	GCWGC	2 cut(s)	415, 2825
ApoI	RAATTY	2 cut(s)	1903, 2633
Asp700I	GAANNNNTTC	2 cut(s)	1101, 2635
AspLEI	GCGC	4 cut(s)	149, 885, 2285, 2687
AspS9I	GGNCC	8 cut(s)	554, 1248, 1444, 1499, 1979, 2375, 2441, 2698
AsuC2I	CCSGG	2 cut(s)	43, 2253
AsuHPI	GGTGA	4 cut(s)	234, 595, 856, 1361
AvaII	GGWCC	7 cut(s)	1248, 1444, 1499, 1979, 2375, 2441, 2698
BaeGI	GKGCMC	1 cut(s)	2871
BanII	GRGCYC	1 cut(s)	120
BauI	CACGAG	1 cut(s)	1414
BbsI	GAAGAC	5 cut(s)	1862, 2561, 2586, 2639, 2765
Bbv12I	GWGCWC	1 cut(s)	2100
BbvI	GCAGC	2 cut(s)	427, 2812
BceAI	ACGGC	4 cut(s)	64, 777, 1494, 1980
BcgI	CGANNNNNNTGC	2 cut(s)	2310, 2344
BciT130I	CCWGG	6 cut(s)	405, 622, 696, 1374, 1396, 2898
BciVI	GTATCC	1 cut(s)	1417
BclI	TGATCA	1 cut(s)	1276
BcnI	CCSGG	2 cut(s)	43, 2253
BcoDI	GTCTC	4 cut(s)	163, 169, 529, 3014
BfaI	CTAG	8 cut(s)	347, 372, 803, 1230, 1521, 1986, 2820, 2906
BfoI	RGCGCY	1 cut(s)	150
BfuI	GTATCC	1 cut(s)	1417
BglI	GCCNNNNNGGC	1 cut(s)	713
BisI	GCNGC	7 cut(s)	378, 416, 945, 1034, 1598, 2286, 2826
BlpI	GCTNAGC	1 cut(s)	2094
BlsI	GCNGC	7 cut(s)	379, 417, 946, 1035, 1599, 2287, 2827
Bme1390I	CCNGG	8 cut(s)	43, 405, 622, 696, 1374, 1396, 2253, 2898
Bme18I	GGWCC	7 cut(s)	1248, 1444, 1499, 1979, 2375, 2441, 2698
BmgBI	CACGTC	1 cut(s)	1879
BmgT120I	GGNCC	8 cut(s)	554, 1248, 1444, 1499, 1979, 2375, 2441, 2698
BmiI	GGNNCC	4 cut(s)	40, 876, 1250, 1500
BmrFI	CCNGG	8 cut(s)	43, 405, 622, 696, 1374, 1396, 2253, 2898
BmsI	GCATC	4 cut(s)	967, 2266, 2297, 2983
BpiI	GAAGAC	5 cut(s)	1862, 2561, 2586, 2639, 2765
BpmI	CTGGAG	3 cut(s)	528, 2437, 2709
Bpu10I	CCTNAGC	2 cut(s)	558, 1092
Bpu1102I	GCTNAGC	1 cut(s)	2094
BpuEI	CTTGAG	2 cut(s)	483, 1731
BpuMI	CCSGG	2 cut(s)	43, 2253
Bsa29I	ATCGAT	2 cut(s)	2085, 2709
BsaBI	GATNNNNATC	2 cut(s)	244, 825
BsaHI	GRCGYC	2 cut(s)	177, 1036
BsaI	GGTCTC	2 cut(s)	529, 3014
BsaJI	CCNNGG	8 cut(s)	122, 403, 771, 1394, 1395, 1469, 2618, 2946
Bsc4I	CCNNNNNNNGG	5 cut(s)	179, 404, 551, 2002, 2952
Bse118I	RCCGGY	1 cut(s)	179
Bse1I	ACTGG	3 cut(s)	671, 1871, 2454
Bse3DI	GCAATG	1 cut(s)	453
Bse8I	GATNNNNATC	2 cut(s)	244, 825
BseBI	CCWGG	6 cut(s)	405, 622, 696, 1374, 1396, 2898
BseCI	ATCGAT	2 cut(s)	2085, 2709
BseDI	CCNNGG	8 cut(s)	122, 403, 771, 1394, 1395, 1469, 2618, 2946
BseGI	GGATG	8 cut(s)	781, 793, 858, 982, 1715, 1888, 2278, 2751
BseJI	GATNNNNATC	2 cut(s)	244, 825
BseLI	CCNNNNNNNGG	5 cut(s)	179, 404, 551, 2002, 2952
BseMI	GCAATG	1 cut(s)	453
BseMII	CTCAG	3 cut(s)	2085, 2145, 2550
BseNI	ACTGG	3 cut(s)	671, 1871, 2454
BseSI	GKGCMC	1 cut(s)	2871
BseXI	GCAGC	2 cut(s)	427, 2812
BseYI	CCCAGC	1 cut(s)	143
Bsh1236I	CGCG	3 cut(s)	124, 2285, 2685
Bsh1285I	CGRYCG	1 cut(s)	62
BshFI	GGCC	7 cut(s)	397, 555, 707, 792, 1372, 2409, 2450
BshVI	ATCGAT	2 cut(s)	2085, 2709
BsiEI	CGRYCG	1 cut(s)	62
BsiHKAI	GWGCWC	1 cut(s)	2100
BsiSI	CCGG	4 cut(s)	43, 100, 180, 2253
BslFI	GGGAC	2 cut(s)	1234, 1512
BslI	CCNNNNNNNGG	5 cut(s)	179, 404, 551, 2002, 2952
BsmAI	GTCTC	4 cut(s)	163, 169, 529, 3014
BsmBI	CGTCTC	2 cut(s)	163, 169
BsmFI	GGGAC	2 cut(s)	1234, 1512
BsmI	GAATGC	2 cut(s)	1669, 2058
BsnI	GGCC	7 cut(s)	397, 555, 707, 792, 1372, 2409, 2450
Bso31I	GGTCTC	2 cut(s)	529, 3014
Bsp1286I	GDGCHC	3 cut(s)	120, 2100, 2871
Bsp1407I	TGTACA	2 cut(s)	1026, 2498
Bsp1720I	GCTNAGC	1 cut(s)	2094
Bsp19I	CCATGG	2 cut(s)	2618, 2946
BspANI	GGCC	7 cut(s)	397, 555, 707, 792, 1372, 2409, 2450
BspCNI	CTCAG	3 cut(s)	2086, 2144, 2551
BspDI	ATCGAT	2 cut(s)	2085, 2709
BspFNI	CGCG	3 cut(s)	124, 2285, 2685
BspHI	TCATGA	2 cut(s)	1189, 2481
BspLI	GGNNCC	4 cut(s)	40, 876, 1250, 1500
BspPI	GGATC	6 cut(s)	608, 713, 1904, 2521, 2705, 2936
BspTNI	GGTCTC	2 cut(s)	529, 3014
BsrBI	CCGCTC	2 cut(s)	36, 380
BsrDI	GCAATG	1 cut(s)	453
BsrFI	RCCGGY	1 cut(s)	179
BsrGI	TGTACA	2 cut(s)	1026, 2498
BsrI	ACTGG	3 cut(s)	671, 1871, 2454
BssAI	RCCGGY	1 cut(s)	179
BssECI	CCNNGG	8 cut(s)	122, 403, 771, 1394, 1395, 1469, 2618, 2946
BssNI	GRCGYC	2 cut(s)	177, 1036
BssSI	CACGAG	1 cut(s)	1414
BssT1I	CCWWGG	3 cut(s)	1469, 2618, 2946
Bst2BI	CACGAG	1 cut(s)	1414
Bst2UI	CCWGG	6 cut(s)	405, 622, 696, 1374, 1396, 2898
Bst4CI	ACNGT	4 cut(s)	1448, 1487, 2066, 2719
BstACI	GRCGYC	2 cut(s)	177, 1036
BstAPI	GCANNNNNTGC	2 cut(s)	322, 1892
BstAUI	TGTACA	2 cut(s)	1026, 2498
BstC8I	GCNNGC	8 cut(s)	181, 234, 399, 1466, 1578, 1669, 2199, 2281
BstDEI	CTNAG	7 cut(s)	528, 558, 1092, 2094, 2131, 2411, 2559
BstDSI	CCRYGG	3 cut(s)	122, 2618, 2946
BstF5I	GGATG	8 cut(s)	781, 793, 858, 982, 1715, 1888, 2278, 2751
BstFNI	CGCG	3 cut(s)	124, 2285, 2685
BstH2I	RGCGCY	1 cut(s)	150
BstHHI	GCGC	4 cut(s)	149, 885, 2285, 2687
BstMAI	GTCTC	4 cut(s)	163, 169, 529, 3014
BstMCI	CGRYCG	1 cut(s)	62
BstNI	CCWGG	6 cut(s)	405, 622, 696, 1374, 1396, 2898
BstNSI	RCATGY	4 cut(s)	325, 1033, 1468, 2656
BstSCI	CCNGG	8 cut(s)	41, 403, 620, 694, 1372, 1394, 2251, 2896
BstSLI	GKGCMC	1 cut(s)	2871
BstUI	CGCG	3 cut(s)	124, 2285, 2685
BstV1I	GCAGC	2 cut(s)	427, 2812
BstV2I	GAAGAC	5 cut(s)	1862, 2561, 2586, 2639, 2765
BstX2I	RGATCY	3 cut(s)	718, 1909, 2941
BstXI	CCANNNNNNTGG	2 cut(s)	1416, 1920
BstYI	RGATCY	3 cut(s)	718, 1909, 2941
Bsu15I	ATCGAT	2 cut(s)	2085, 2709
BsuI	GTATCC	1 cut(s)	1417
BsuRI	GGCC	7 cut(s)	397, 555, 707, 792, 1372, 2409, 2450
BsuTUI	ATCGAT	2 cut(s)	2085, 2709
BtgI	CCRYGG	3 cut(s)	122, 2618, 2946
BtrI	CACGTC	1 cut(s)	1879
BtsCI	GGATG	8 cut(s)	781, 793, 858, 982, 1715, 1888, 2278, 2751
BtsIMutI	CAGTG	3 cut(s)	1483, 2030, 2071
Cac8I	GCNNGC	8 cut(s)	181, 234, 399, 1466, 1578, 1669, 2199, 2281
CaiI	CAGNNNCTG	1 cut(s)	404
CciI	TCATGA	2 cut(s)	1189, 2481
CfoI	GCGC	4 cut(s)	149, 885, 2285, 2687
Cfr10I	RCCGGY	1 cut(s)	179
Cfr13I	GGNCC	8 cut(s)	554, 1248, 1444, 1499, 1979, 2375, 2441, 2698
Cfr42I	CCGCGG	1 cut(s)	125
ClaI	ATCGAT	2 cut(s)	2085, 2709
CseI	GACGC	2 cut(s)	185, 1025
Csp6I	GTAC	5 cut(s)	761, 1027, 2249, 2499, 2798
CviQI	GTAC	5 cut(s)	761, 1027, 2249, 2499, 2798
DdeI	CTNAG	7 cut(s)	528, 558, 1092, 2094, 2131, 2411, 2559
DrdI	GACNNNNNNGTC	1 cut(s)	1977
DseDI	GACNNNNNNGTC	1 cut(s)	1977
EaeI	YGGCCR	2 cut(s)	395, 790
Eco130I	CCWWGG	3 cut(s)	1469, 2618, 2946
Eco24I	GRGCYC	1 cut(s)	120
Eco31I	GGTCTC	2 cut(s)	529, 3014
Eco32I	GATATC	4 cut(s)	21, 187, 458, 1729
Eco47I	GGWCC	7 cut(s)	1248, 1444, 1499, 1979, 2375, 2441, 2698
Eco57I	CTGAAG	1 cut(s)	924
EcoO109I	RGGNCCY	1 cut(s)	1499
EcoRII	CCWGG	6 cut(s)	403, 620, 694, 1372, 1394, 2896
EcoRV	GATATC	4 cut(s)	21, 187, 458, 1729
EcoT14I	CCWWGG	3 cut(s)	1469, 2618, 2946
EcoT22I	ATGCAT	2 cut(s)	844, 2310
EcoT38I	GRGCYC	1 cut(s)	120
ErhI	CCWWGG	3 cut(s)	1469, 2618, 2946
Esp3I	CGTCTC	2 cut(s)	163, 169
FalI	AAGNNNNNCTT	2 cut(s)	444, 476
FaqI	GGGAC	2 cut(s)	1234, 1512
FauI	CCCGC	2 cut(s)	129, 176
FauNDI	CATATG	1 cut(s)	1783
FbaI	TGATCA	1 cut(s)	1276
FblI	GTMKAC	1 cut(s)	1875
Fnu4HI	GCNGC	7 cut(s)	378, 416, 945, 1034, 1598, 2286, 2826
FokI	GGATG	8 cut(s)	788, 800, 845, 989, 1702, 1875, 2285, 2758
FriOI	GRGCYC	1 cut(s)	120
Fsp4HI	GCNGC	7 cut(s)	378, 416, 945, 1034, 1598, 2286, 2826
FspBI	CTAG	8 cut(s)	347, 372, 803, 1230, 1521, 1986, 2820, 2906
GlaI	GCGC	4 cut(s)	148, 884, 2284, 2686
GluI	GCNGC	7 cut(s)	378, 416, 945, 1034, 1598, 2286, 2826
GsaI	CCCAGC	1 cut(s)	147
GsuI	CTGGAG	3 cut(s)	528, 2437, 2709
HaeII	RGCGCY	1 cut(s)	150
HaeIII	GGCC	7 cut(s)	397, 555, 707, 792, 1372, 2409, 2450
HapII	CCGG	4 cut(s)	43, 100, 180, 2253
HgaI	GACGC	2 cut(s)	185, 1025
HhaI	GCGC	4 cut(s)	149, 885, 2285, 2687
Hin1I	GRCGYC	2 cut(s)	177, 1036
Hin6I	GCGC	4 cut(s)	147, 883, 2283, 2685
HinP1I	GCGC	4 cut(s)	147, 883, 2283, 2685
HincII	GTYRAC	1 cut(s)	72
HindII	GTYRAC	1 cut(s)	72
HpaI	GTTAAC	1 cut(s)	72
HpaII	CCGG	4 cut(s)	43, 100, 180, 2253
HphI	GGTGA	4 cut(s)	234, 595, 856, 1361
Hpy166II	GTNNAC	8 cut(s)	72, 493, 1240, 1483, 1792, 1876, 2426, 2798
Hpy8I	GTNNAC	8 cut(s)	72, 493, 1240, 1483, 1792, 1876, 2426, 2798
Hpy99I	CGWCG	7 cut(s)	31, 79, 85, 88, 137, 179, 1711
HpyAV	CCTTC	7 cut(s)	121, 627, 1091, 1992, 2617, 2848, 2854
HpyCH4III	ACNGT	4 cut(s)	1448, 1487, 2066, 2719
HpyCH4IV	ACGT	3 cut(s)	74, 1210, 1878
HpyF3I	CTNAG	7 cut(s)	528, 558, 1092, 2094, 2131, 2411, 2559
HpySE526I	ACGT	3 cut(s)	74, 1210, 1878
Hsp92I	GRCGYC	2 cut(s)	177, 1036
HspAI	GCGC	4 cut(s)	147, 883, 2283, 2685
KroI	GCCGGC	1 cut(s)	179
KroNI	GCCGGC	1 cut(s)	181
Ksp22I	TGATCA	1 cut(s)	1276
KspAI	GTTAAC	1 cut(s)	72
KspI	CCGCGG	1 cut(s)	125
LmnI	GCTCC	6 cut(s)	10, 33, 64, 880, 1570, 2899
Lsp1109I	GCAGC	2 cut(s)	427, 2812
LweI	GCATC	4 cut(s)	967, 2266, 2297, 2983
MaeI	CTAG	8 cut(s)	347, 372, 803, 1230, 1521, 1986, 2820, 2906
MaeII	ACGT	3 cut(s)	74, 1210, 1878
MaeIII	GTNAC	1 cut(s)	1349
MbiI	CCGCTC	2 cut(s)	36, 380
MfeI	CAATTG	1 cut(s)	969
MflI	RGATCY	3 cut(s)	718, 1909, 2941
MhlI	GDGCHC	3 cut(s)	120, 2100, 2871
MlyI	GAGTC	4 cut(s)	1787, 2653, 2918, 2969
MmeI	TCCRAC	4 cut(s)	1276, 1422, 1807, 2738
Mph1103I	ATGCAT	2 cut(s)	844, 2310
MreI	CGCCGGCG	1 cut(s)	179
MroNI	GCCGGC	1 cut(s)	179
MroXI	GAANNNNTTC	2 cut(s)	1101, 2635
MseI	TTAA	5 cut(s)	71, 462, 689, 1686, 2595
MslI	CAYNNNNRTG	1 cut(s)	1194
MspA1I	CMGCKG	2 cut(s)	124, 1537
MspI	CCGG	4 cut(s)	43, 100, 180, 2253
MspR9I	CCNGG	8 cut(s)	43, 405, 622, 696, 1374, 1396, 2253, 2898
MunI	CAATTG	1 cut(s)	969
Mva1269I	GAATGC	2 cut(s)	1669, 2058
MvaI	CCWGG	6 cut(s)	405, 622, 696, 1374, 1396, 2898
MvnI	CGCG	3 cut(s)	124, 2285, 2685
NaeI	GCCGGC	1 cut(s)	181
NciI	CCSGG	2 cut(s)	43, 2253
NcoI	CCATGG	2 cut(s)	2618, 2946
NdeI	CATATG	1 cut(s)	1783
NgoMIV	GCCGGC	1 cut(s)	179
NlaIV	GGNNCC	4 cut(s)	40, 876, 1250, 1500
NmuCI	GTSAC	1 cut(s)	1349
NsiI	ATGCAT	2 cut(s)	844, 2310
NspI	RCATGY	4 cut(s)	325, 1033, 1468, 2656
PaeI	GCATGC	1 cut(s)	1468
PagI	TCATGA	2 cut(s)	1189, 2481
PasI	CCCWGGG	1 cut(s)	1395
PcsI	WCGNNNNNNNCGW	4 cut(s)	80, 92, 2194, 2909
PctI	GAATGC	2 cut(s)	1669, 2058
PdiI	GCCGGC	1 cut(s)	181
PdmI	GAANNNNTTC	2 cut(s)	1101, 2635
PflMI	CCANNNNNTGG	2 cut(s)	404, 551
PfoI	TCCNGGA	2 cut(s)	620, 2896
PkrI	GCNGC	7 cut(s)	379, 417, 946, 1035, 1599, 2287, 2827
Ple19I	CGATCG	1 cut(s)	62
PleI	GAGTC	4 cut(s)	1787, 2652, 2917, 2968
PpsI	GAGTC	4 cut(s)	1787, 2652, 2917, 2968
PpuMI	RGGWCCY	1 cut(s)	1499
PsiI	TTATAA	1 cut(s)	2231
Psp5II	RGGWCCY	1 cut(s)	1499
Psp6I	CCWGG	6 cut(s)	403, 620, 694, 1372, 1394, 2896
PspFI	CCCAGC	1 cut(s)	143
PspGI	CCWGG	6 cut(s)	403, 620, 694, 1372, 1394, 2896
PspN4I	GGNNCC	4 cut(s)	40, 876, 1250, 1500
PspPI	GGNCC	8 cut(s)	554, 1248, 1444, 1499, 1979, 2375, 2441, 2698
PspPPI	RGGWCCY	1 cut(s)	1499
PstNI	CAGNNNCTG	1 cut(s)	404
PsuI	RGATCY	3 cut(s)	718, 1909, 2941
PvuI	CGATCG	1 cut(s)	62
RsaI	GTAC	5 cut(s)	762, 1028, 2250, 2500, 2799
RsaNI	GTAC	5 cut(s)	761, 1027, 2249, 2499, 2798
RseI	CAYNNNNRTG	1 cut(s)	1194
SacII	CCGCGG	1 cut(s)	125
SaqAI	TTAA	5 cut(s)	71, 462, 689, 1686, 2595
SatI	GCNGC	7 cut(s)	378, 416, 945, 1034, 1598, 2286, 2826
Sau96I	GGNCC	8 cut(s)	554, 1248, 1444, 1499, 1979, 2375, 2441, 2698
SchI	GAGTC	4 cut(s)	1787, 2653, 2918, 2969
ScrFI	CCNGG	8 cut(s)	43, 405, 622, 696, 1374, 1396, 2253, 2898
SduI	GDGCHC	3 cut(s)	120, 2100, 2871
SfaNI	GCATC	4 cut(s)	967, 2266, 2297, 2983
Sfr303I	CCGCGG	1 cut(s)	125
SgrAI	CRCCGGYG	1 cut(s)	179
SgrBI	CCGCGG	1 cut(s)	125
SinI	GGWCC	7 cut(s)	1248, 1444, 1499, 1979, 2375, 2441, 2698
SmiMI	CAYNNNNRTG	1 cut(s)	1194
SmlI	CTYRAG	2 cut(s)	498, 1746
SmoI	CTYRAG	2 cut(s)	498, 1746
SphI	GCATGC	1 cut(s)	1468
SspMI	CTAG	8 cut(s)	347, 372, 803, 1230, 1521, 1986, 2820, 2906
StyD4I	CCNGG	8 cut(s)	41, 403, 620, 694, 1372, 1394, 2251, 2896
StyI	CCWWGG	3 cut(s)	1469, 2618, 2946
TaaI	ACNGT	4 cut(s)	1448, 1487, 2066, 2719
TaiI	ACGT	3 cut(s)	77, 1213, 1881
TatI	WGTACW	3 cut(s)	760, 1026, 2498
TauI	GCSGC	5 cut(s)	380, 947, 1036, 1600, 2288
Tru1I	TTAA	5 cut(s)	71, 462, 689, 1686, 2595
Tru9I	TTAA	5 cut(s)	71, 462, 689, 1686, 2595
TscAI	CASTG	3 cut(s)	1490, 2030, 2071
TseFI	GTSAC	1 cut(s)	1349
TseI	GCWGC	2 cut(s)	415, 2825
Tsp45I	GTSAC	1 cut(s)	1349
TspGWI	ACGGA	3 cut(s)	151, 1029, 2173
TspRI	CASTG	3 cut(s)	1490, 2030, 2071
Van91I	CCANNNNNTGG	2 cut(s)	404, 551
VpaK11BI	GGWCC	7 cut(s)	1248, 1444, 1499, 1979, 2375, 2441, 2698
XapI	RAATTY	2 cut(s)	1903, 2633
XbaI	TCTAGA	1 cut(s)	2819
XceI	RCATGY	4 cut(s)	325, 1033, 1468, 2656
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1229
XmiI	GTMKAC	1 cut(s)	1875
XmnI	GAANNNNTTC	2 cut(s)	1101, 2635
XspI	CTAG	8 cut(s)	347, 372, 803, 1230, 1521, 1986, 2820, 2906
Zsp2I	ATGCAT	2 cut(s)	844, 2310

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.