phospholipase D
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 283 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 1656, 2139 |
| AatII | GACGTC | 1 cut(s) | 1189 |
| Acc36I | ACCTGC | 1 cut(s) | 283 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 2493 |
| AccI | GTMKAC | 1 cut(s) | 1905 |
| AccII | CGCG | 2 cut(s) | 127, 1612 |
| AciI | CCGC | 8 cut(s) | 32, 62, 127, 202, 1471, 1610, 1652, 2447 |
| AcoI | YGGCCR | 4 cut(s) | 105, 1087, 1468, 1520 |
| AcsI | RAATTY | 7 cut(s) | 541, 560, 1561, 2035, 2795, 2926, 3203 |
| AcuI | CTGAAG | 2 cut(s) | 230, 2232 |
| AcyI | GRCGYC | 1 cut(s) | 1186 |
| AdeI | CACNNNGTG | 1 cut(s) | 1495 |
| AfaI | GTAC | 7 cut(s) | 36, 384, 810, 1313, 2149, 2345, 2631 |
| AfeI | AGCGCT | 1 cut(s) | 1688 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 137, 368, 455, 998, 2164, 2332, 2493 |
| AflII | CTTAAG | 1 cut(s) | 919 |
| AjnI | CCWGG | 8 cut(s) | 742, 750, 1522, 1670, 1923, 2331, 2413, 2835 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1574, 1606 |
| Alw21I | GWGCWC | 2 cut(s) | 2819, 2835 |
| Alw26I | GTCTC | 4 cut(s) | 426, 577, 2709, 3175 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 659 |
| Ama87I | CYCGRG | 1 cut(s) | 546 |
| Aor51HI | AGCGCT | 1 cut(s) | 1688 |
| AoxI | GGCC | 9 cut(s) | 105, 128, 753, 989, 1087, 1468, 1520, 2059, 2787 |
| ApeKI | GCWGC | 9 cut(s) | 205, 428, 463, 965, 1094, 1127, 1951, 2117, 2987 |
| ApoI | RAATTY | 7 cut(s) | 541, 560, 1561, 2035, 2795, 2926, 3203 |
| AseI | ATTAAT | 1 cut(s) | 972 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 306, 2282 |
| AspLEI | GCGC | 3 cut(s) | 1362, 1689, 1922 |
| AspS9I | GGNCC | 6 cut(s) | 129, 602, 1649, 2141, 2537, 2603 |
| AsuHPI | GGTGA | 6 cut(s) | 307, 643, 1511, 1833, 2401, 3103 |
| AsuII | TTCGAA | 2 cut(s) | 90, 2595 |
| AsuNHI | GCTAGC | 1 cut(s) | 2359 |
| AvaI | CYCGRG | 1 cut(s) | 546 |
| AvaII | GGWCC | 5 cut(s) | 602, 1649, 2141, 2537, 2603 |
| BaeGI | GKGCMC | 2 cut(s) | 1642, 3033 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 99, 132 |
| BalI | TGGCCA | 3 cut(s) | 107, 1089, 1522 |
| BamHI | GGATCC | 1 cut(s) | 766 |
| BanII | GRGCYC | 1 cut(s) | 2464 |
| BarI | GAAGNNNNNNTAC | 6 cut(s) | 1192, 1224, 1989, 2021, 2205, 2237 |
| BauI | CACGAG | 1 cut(s) | 423 |
| BbsI | GAAGAC | 2 cut(s) | 1578, 2834 |
| Bbv12I | GWGCWC | 2 cut(s) | 2819, 2835 |
| BbvI | GCAGC | 9 cut(s) | 192, 415, 475, 952, 1106, 1114, 1963, 2104, 2974 |
| BccI | CCATC | 7 cut(s) | 362, 446, 1052, 1079, 1877, 1978, 2432 |
| BceAI | ACGGC | 2 cut(s) | 351, 1603 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 2426, 2460, 2575, 2609 |
| BciT130I | CCWGG | 8 cut(s) | 744, 752, 1524, 1672, 1925, 2333, 2415, 2837 |
| BciVI | GTATCC | 1 cut(s) | 2620 |
| BclI | TGATCA | 1 cut(s) | 2104 |
| BcoDI | GTCTC | 4 cut(s) | 426, 577, 2709, 3175 |
| BfaI | CTAG | 8 cut(s) | 455, 536, 761, 936, 1242, 1454, 2202, 2360 |
| BfmI | CTRYAG | 1 cut(s) | 703 |
| BfoI | RGCGCY | 2 cut(s) | 1690, 1923 |
| BfrI | CTTAAG | 1 cut(s) | 919 |
| BfuAI | ACCTGC | 1 cut(s) | 283 |
| BfuI | GTATCC | 1 cut(s) | 2620 |
| BglII | AGATCT | 1 cut(s) | 2899 |
| Bme1390I | CCNGG | 8 cut(s) | 744, 752, 1524, 1672, 1925, 2333, 2415, 2837 |
| Bme18I | GGWCC | 5 cut(s) | 602, 1649, 2141, 2537, 2603 |
| BmeT110I | CYCGRG | 1 cut(s) | 546 |
| BmgT120I | GGNCC | 6 cut(s) | 129, 602, 1649, 2141, 2537, 2603 |
| BmiI | GGNNCC | 6 cut(s) | 749, 768, 1027, 1064, 1650, 1694 |
| BmrFI | CCNGG | 8 cut(s) | 744, 752, 1524, 1672, 1925, 2333, 2415, 2837 |
| BmsI | GCATC | 6 cut(s) | 74, 227, 646, 692, 967, 1210 |
| BmtI | GCTAGC | 1 cut(s) | 2363 |
| BoxI | GACNNNNGTC | 1 cut(s) | 2697 |
| BpiI | GAAGAC | 2 cut(s) | 1578, 2834 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 1054, 1086, 2976, 3008 |
| Bpu14I | TTCGAA | 2 cut(s) | 90, 2595 |
| BpuEI | CTTGAG | 4 cut(s) | 1857, 1967, 2965, 3204 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1145 |
| BsaHI | GRCGYC | 1 cut(s) | 1186 |
| BsaI | GGTCTC | 2 cut(s) | 577, 3175 |
| BsaJI | CCNNGG | 8 cut(s) | 42, 743, 1040, 1380, 1619, 1923, 2780, 2836 |
| BsaWI | WCCGGW | 2 cut(s) | 137, 361 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 137, 368, 455, 998, 2164, 2332, 2493 |
| Bse118I | RCCGGY | 1 cut(s) | 100 |
| Bse1I | ACTGG | 4 cut(s) | 719, 1156, 2185, 3185 |
| Bse3DI | GCAATG | 5 cut(s) | 18, 54, 501, 946, 1665 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1145 |
| BseBI | CCWGG | 8 cut(s) | 744, 752, 1524, 1672, 1925, 2333, 2415, 2837 |
| BseDI | CCNNGG | 8 cut(s) | 42, 743, 1040, 1380, 1619, 1923, 2780, 2836 |
| BseGI | GGATG | 7 cut(s) | 370, 438, 683, 1009, 1764, 2314, 3212 |
| BseJI | GATNNNNATC | 1 cut(s) | 1145 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 137, 368, 455, 998, 2164, 2332, 2493 |
| BseMI | GCAATG | 5 cut(s) | 18, 54, 501, 946, 1665 |
| BseMII | CTCAG | 4 cut(s) | 2564, 2712, 2820, 3007 |
| BseNI | ACTGG | 4 cut(s) | 719, 1156, 2185, 3185 |
| BseRI | GAGGAG | 2 cut(s) | 284, 1500 |
| BseSI | GKGCMC | 2 cut(s) | 1642, 3033 |
| BseX3I | CGGCCG | 1 cut(s) | 1468 |
| BseXI | GCAGC | 9 cut(s) | 192, 415, 475, 952, 1106, 1114, 1963, 2104, 2974 |
| BseYI | CCCAGC | 1 cut(s) | 2493 |
| Bsh1236I | CGCG | 2 cut(s) | 127, 1612 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1471 |
| BshFI | GGCC | 9 cut(s) | 107, 130, 755, 991, 1089, 1470, 1522, 2061, 2789 |
| BsiEI | CGRYCG | 1 cut(s) | 1471 |
| BsiHKAI | GWGCWC | 2 cut(s) | 2819, 2835 |
| BsiHKCI | CYCGRG | 1 cut(s) | 546 |
| BsiSI | CCGG | 4 cut(s) | 101, 138, 362, 2790 |
| BslFI | GGGAC | 2 cut(s) | 1662, 3147 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 137, 368, 455, 998, 2164, 2332, 2493 |
| BsmAI | GTCTC | 4 cut(s) | 426, 577, 2709, 3175 |
| BsmBI | CGTCTC | 1 cut(s) | 426 |
| BsmFI | GGGAC | 2 cut(s) | 1662, 3147 |
| BsmI | GAATGC | 1 cut(s) | 64 |
| BsnI | GGCC | 9 cut(s) | 107, 130, 755, 991, 1089, 1470, 1522, 2061, 2789 |
| Bso31I | GGTCTC | 2 cut(s) | 577, 3175 |
| BsoBI | CYCGRG | 1 cut(s) | 546 |
| Bsp119I | TTCGAA | 2 cut(s) | 90, 2595 |
| Bsp1286I | GDGCHC | 5 cut(s) | 1642, 2464, 2819, 2835, 3033 |
| Bsp1407I | TGTACA | 3 cut(s) | 382, 2343, 2629 |
| BspACI | CCGC | 8 cut(s) | 32, 62, 127, 202, 1471, 1610, 1652, 2447 |
| BspANI | GGCC | 9 cut(s) | 107, 130, 755, 991, 1089, 1470, 1522, 2061, 2789 |
| BspCNI | CTCAG | 4 cut(s) | 2565, 2713, 2821, 3006 |
| BspFNI | CGCG | 2 cut(s) | 127, 1612 |
| BspHI | TCATGA | 4 cut(s) | 277, 994, 1339, 1405 |
| BspLI | GGNNCC | 6 cut(s) | 749, 768, 1027, 1064, 1650, 1694 |
| BspMI | ACCTGC | 1 cut(s) | 283 |
| BspOI | GCTAGC | 1 cut(s) | 2363 |
| BspT104I | TTCGAA | 2 cut(s) | 90, 2595 |
| BspTI | CTTAAG | 1 cut(s) | 919 |
| BspTNI | GGTCTC | 2 cut(s) | 577, 3175 |
| BsrDI | GCAATG | 5 cut(s) | 18, 54, 501, 946, 1665 |
| BsrFI | RCCGGY | 1 cut(s) | 100 |
| BsrGI | TGTACA | 3 cut(s) | 382, 2343, 2629 |
| BsrI | ACTGG | 4 cut(s) | 719, 1156, 2185, 3185 |
| BssAI | RCCGGY | 1 cut(s) | 100 |
| BssECI | CCNNGG | 8 cut(s) | 42, 743, 1040, 1380, 1619, 1923, 2780, 2836 |
| BssNI | GRCGYC | 1 cut(s) | 1186 |
| BssSI | CACGAG | 1 cut(s) | 423 |
| BssT1I | CCWWGG | 2 cut(s) | 1040, 2780 |
| Bst2BI | CACGAG | 1 cut(s) | 423 |
| Bst2UI | CCWGG | 8 cut(s) | 744, 752, 1524, 1672, 1925, 2333, 2415, 2837 |
| Bst4CI | ACNGT | 9 cut(s) | 192, 1176, 1307, 1316, 1373, 1483, 1845, 2327, 2455 |
| Bst6I | CTCTTC | 1 cut(s) | 87 |
| BstACI | GRCGYC | 1 cut(s) | 1186 |
| BstAFI | CTTAAG | 1 cut(s) | 919 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 29 |
| BstAUI | TGTACA | 3 cut(s) | 382, 2343, 2629 |
| BstBI | TTCGAA | 2 cut(s) | 90, 2595 |
| BstC8I | GCNNGC | 4 cut(s) | 258, 835, 2155, 2361 |
| BstDEI | CTNAG | 8 cut(s) | 1181, 1701, 2541, 2573, 2721, 2810, 2829, 2993 |
| BstDSI | CCRYGG | 2 cut(s) | 1380, 1619 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 996 |
| BstF5I | GGATG | 7 cut(s) | 370, 438, 683, 1009, 1764, 2314, 3212 |
| BstFNI | CGCG | 2 cut(s) | 127, 1612 |
| BstH2I | RGCGCY | 2 cut(s) | 1690, 1923 |
| BstHHI | GCGC | 3 cut(s) | 1362, 1689, 1922 |
| BstMAI | GTCTC | 4 cut(s) | 426, 577, 2709, 3175 |
| BstMCI | CGRYCG | 1 cut(s) | 1471 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 29, 491, 1103, 1470, 2117, 2356 |
| BstNI | CCWGG | 8 cut(s) | 744, 752, 1524, 1672, 1925, 2333, 2415, 2837 |
| BstNSI | RCATGY | 3 cut(s) | 837, 2157, 3128 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 2697 |
| BstSCI | CCNGG | 8 cut(s) | 742, 750, 1522, 1670, 1923, 2331, 2413, 2835 |
| BstSFI | CTRYAG | 1 cut(s) | 703 |
| BstSLI | GKGCMC | 2 cut(s) | 1642, 3033 |
| BstUI | CGCG | 2 cut(s) | 127, 1612 |
| BstV1I | GCAGC | 9 cut(s) | 192, 415, 475, 952, 1106, 1114, 1963, 2104, 2974 |
| BstV2I | GAAGAC | 2 cut(s) | 1578, 2834 |
| BstX2I | RGATCY | 6 cut(s) | 532, 766, 2017, 2198, 2899, 3103 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1566 |
| BstYI | RGATCY | 6 cut(s) | 532, 766, 2017, 2198, 2899, 3103 |
| BstZI | CGGCCG | 1 cut(s) | 1468 |
| BsuI | GTATCC | 1 cut(s) | 2620 |
| BsuRI | GGCC | 9 cut(s) | 107, 130, 755, 991, 1089, 1470, 1522, 2061, 2789 |
| BtgI | CCRYGG | 2 cut(s) | 1380, 1619 |
| BtgZI | GCGATG | 1 cut(s) | 1001 |
| BtsCI | GGATG | 7 cut(s) | 370, 438, 683, 1009, 1764, 2314, 3212 |
| BtsIMutI | CAGTG | 5 cut(s) | 777, 1149, 2192, 2875, 3192 |
| BveI | ACCTGC | 1 cut(s) | 283 |
| Cac8I | GCNNGC | 4 cut(s) | 258, 835, 2155, 2361 |
| CaiI | CAGNNNCTG | 1 cut(s) | 659 |
| CciI | TCATGA | 4 cut(s) | 277, 994, 1339, 1405 |
| CfoI | GCGC | 3 cut(s) | 1362, 1689, 1922 |
| Cfr10I | RCCGGY | 1 cut(s) | 100 |
| Cfr13I | GGNCC | 6 cut(s) | 129, 602, 1649, 2141, 2537, 2603 |
| Csp6I | GTAC | 7 cut(s) | 35, 383, 809, 1312, 2148, 2344, 2630 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 707, 742 |
| CviQI | GTAC | 7 cut(s) | 35, 383, 809, 1312, 2148, 2344, 2630 |
| DdeI | CTNAG | 8 cut(s) | 1181, 1701, 2541, 2573, 2721, 2810, 2829, 2993 |
| DraI | TTTAAA | 4 cut(s) | 738, 1296, 1890, 2040 |
| DraIII | CACNNNGTG | 1 cut(s) | 1495 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 1656, 2139 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 1656, 2139 |
| EaeI | YGGCCR | 4 cut(s) | 105, 1087, 1468, 1520 |
| EagI | CGGCCG | 1 cut(s) | 1468 |
| Eam1104I | CTCTTC | 1 cut(s) | 87 |
| EarI | CTCTTC | 1 cut(s) | 87 |
| EclXI | CGGCCG | 1 cut(s) | 1468 |
| Eco130I | CCWWGG | 2 cut(s) | 1040, 2780 |
| Eco147I | AGGCCT | 1 cut(s) | 991 |
| Eco24I | GRGCYC | 1 cut(s) | 2464 |
| Eco31I | GGTCTC | 2 cut(s) | 577, 3175 |
| Eco32I | GATATC | 2 cut(s) | 145, 1607 |
| Eco47I | GGWCC | 5 cut(s) | 602, 1649, 2141, 2537, 2603 |
| Eco47III | AGCGCT | 1 cut(s) | 1688 |
| Eco52I | CGGCCG | 1 cut(s) | 1468 |
| Eco57I | CTGAAG | 2 cut(s) | 230, 2232 |
| Eco88I | CYCGRG | 1 cut(s) | 546 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 996 |
| EcoO109I | RGGNCCY | 1 cut(s) | 2141 |
| EcoRI | GAATTC | 1 cut(s) | 541 |
| EcoRII | CCWGG | 8 cut(s) | 742, 750, 1522, 1670, 1923, 2331, 2413, 2835 |
| EcoRV | GATATC | 2 cut(s) | 145, 1607 |
| EcoT14I | CCWWGG | 2 cut(s) | 1040, 2780 |
| EcoT22I | ATGCAT | 4 cut(s) | 835, 1225, 2472, 3005 |
| EcoT38I | GRGCYC | 1 cut(s) | 2464 |
| ErhI | CCWWGG | 2 cut(s) | 1040, 2780 |
| Esp3I | CGTCTC | 1 cut(s) | 426 |
| FaqI | GGGAC | 2 cut(s) | 1662, 3147 |
| FbaI | TGATCA | 1 cut(s) | 2104 |
| FblI | GTMKAC | 1 cut(s) | 1905 |
| FokI | GGATG | 7 cut(s) | 377, 425, 670, 996, 1751, 2321, 3199 |
| FriOI | GRGCYC | 1 cut(s) | 2464 |
| FspBI | CTAG | 8 cut(s) | 455, 536, 761, 936, 1242, 1454, 2202, 2360 |
| GlaI | GCGC | 3 cut(s) | 1361, 1688, 1921 |
| GsaI | CCCAGC | 1 cut(s) | 2497 |
| HaeII | RGCGCY | 2 cut(s) | 1690, 1923 |
| HaeIII | GGCC | 9 cut(s) | 107, 130, 755, 991, 1089, 1470, 1522, 2061, 2789 |
| HapII | CCGG | 4 cut(s) | 101, 138, 362, 2790 |
| HhaI | GCGC | 3 cut(s) | 1362, 1689, 1922 |
| Hin1I | GRCGYC | 1 cut(s) | 1186 |
| Hin6I | GCGC | 3 cut(s) | 1360, 1687, 1920 |
| HinP1I | GCGC | 3 cut(s) | 1360, 1687, 1920 |
| HindIII | AAGCTT | 2 cut(s) | 916, 2229 |
| HinfI | GANTC | 7 cut(s) | 511, 662, 1550, 2278, 2717, 2759, 2969 |
| HpaII | CCGG | 4 cut(s) | 101, 138, 362, 2790 |
| HphI | GGTGA | 6 cut(s) | 307, 643, 1511, 1833, 2401, 3103 |
| Hpy166II | GTNNAC | 4 cut(s) | 673, 1906, 2434, 2632 |
| Hpy8I | GTNNAC | 4 cut(s) | 673, 1906, 2434, 2632 |
| Hpy99I | CGWCG | 2 cut(s) | 10, 1849 |
| HpyCH4III | ACNGT | 9 cut(s) | 192, 1176, 1307, 1316, 1373, 1483, 1845, 2327, 2455 |
| HpyCH4IV | ACGT | 4 cut(s) | 414, 883, 1186, 1800 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 29, 491, 1103, 1470, 2117, 2356 |
| HpyF3I | CTNAG | 8 cut(s) | 1181, 1701, 2541, 2573, 2721, 2810, 2829, 2993 |
| HpySE526I | ACGT | 4 cut(s) | 414, 883, 1186, 1800 |
| Hsp92I | GRCGYC | 1 cut(s) | 1186 |
| HspAI | GCGC | 3 cut(s) | 1360, 1687, 1920 |
| Ksp22I | TGATCA | 1 cut(s) | 2104 |
| LmnI | GCTCC | 4 cut(s) | 1062, 1594, 1720, 1900 |
| Lsp1109I | GCAGC | 9 cut(s) | 192, 415, 475, 952, 1106, 1114, 1963, 2104, 2974 |
| LweI | GCATC | 6 cut(s) | 74, 227, 646, 692, 967, 1210 |
| MaeI | CTAG | 8 cut(s) | 455, 536, 761, 936, 1242, 1454, 2202, 2360 |
| MaeII | ACGT | 4 cut(s) | 414, 883, 1186, 1800 |
| MaeIII | GTNAC | 4 cut(s) | 884, 1398, 1499, 1661 |
| MfeI | CAATTG | 1 cut(s) | 3039 |
| MflI | RGATCY | 6 cut(s) | 532, 766, 2017, 2198, 2899, 3103 |
| MhlI | GDGCHC | 5 cut(s) | 1642, 2464, 2819, 2835, 3033 |
| MlsI | TGGCCA | 3 cut(s) | 107, 1089, 1522 |
| MluNI | TGGCCA | 3 cut(s) | 107, 1089, 1522 |
| MlyI | GAGTC | 1 cut(s) | 2711 |
| MmeI | TCCRAC | 1 cut(s) | 2064 |
| Mox20I | TGGCCA | 3 cut(s) | 107, 1089, 1522 |
| Mph1103I | ATGCAT | 4 cut(s) | 835, 1225, 2472, 3005 |
| MroXI | GAANNNNTTC | 2 cut(s) | 306, 2282 |
| MscI | TGGCCA | 3 cut(s) | 107, 1089, 1522 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1344, 2106 |
| Msp20I | TGGCCA | 3 cut(s) | 107, 1089, 1522 |
| MspCI | CTTAAG | 1 cut(s) | 919 |
| MspI | CCGG | 4 cut(s) | 101, 138, 362, 2790 |
| MspR9I | CCNGG | 8 cut(s) | 744, 752, 1524, 1672, 1925, 2333, 2415, 2837 |
| MunI | CAATTG | 1 cut(s) | 3039 |
| Mva1269I | GAATGC | 1 cut(s) | 64 |
| MvaI | CCWGG | 8 cut(s) | 744, 752, 1524, 1672, 1925, 2333, 2415, 2837 |
| MvnI | CGCG | 2 cut(s) | 127, 1612 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 29, 491, 1103, 1470, 2117, 2356 |
| NheI | GCTAGC | 1 cut(s) | 2359 |
| NlaIV | GGNNCC | 6 cut(s) | 749, 768, 1027, 1064, 1650, 1694 |
| NmeAIII | GCCGAG | 1 cut(s) | 1192 |
| NmuCI | GTSAC | 3 cut(s) | 1398, 1499, 1661 |
| NsiI | ATGCAT | 4 cut(s) | 835, 1225, 2472, 3005 |
| NspI | RCATGY | 3 cut(s) | 837, 2157, 3128 |
| NspV | TTCGAA | 2 cut(s) | 90, 2595 |
| PaeI | GCATGC | 2 cut(s) | 837, 2157 |
| PaeR7I | CTCGAG | 1 cut(s) | 546 |
| PagI | TCATGA | 4 cut(s) | 277, 994, 1339, 1405 |
| PaqCI | CACCTGC | 1 cut(s) | 283 |
| PceI | AGGCCT | 1 cut(s) | 991 |
| PctI | GAATGC | 1 cut(s) | 64 |
| PdmI | GAANNNNTTC | 2 cut(s) | 306, 2282 |
| PfeI | GAWTC | 6 cut(s) | 511, 662, 1550, 2278, 2759, 2969 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 2493 |
| PfoI | TCCNGGA | 1 cut(s) | 2331 |
| PleI | GAGTC | 1 cut(s) | 2711 |
| PpsI | GAGTC | 1 cut(s) | 2711 |
| PpuMI | RGGWCCY | 1 cut(s) | 2141 |
| PshAI | GACNNNNGTC | 1 cut(s) | 2697 |
| PshBI | ATTAAT | 1 cut(s) | 972 |
| Psp5II | RGGWCCY | 1 cut(s) | 2141 |
| Psp6I | CCWGG | 8 cut(s) | 742, 750, 1522, 1670, 1923, 2331, 2413, 2835 |
| PspFI | CCCAGC | 1 cut(s) | 2493 |
| PspGI | CCWGG | 8 cut(s) | 742, 750, 1522, 1670, 1923, 2331, 2413, 2835 |
| PspN4I | GGNNCC | 6 cut(s) | 749, 768, 1027, 1064, 1650, 1694 |
| PspPI | GGNCC | 6 cut(s) | 129, 602, 1649, 2141, 2537, 2603 |
| PspPPI | RGGWCCY | 1 cut(s) | 2141 |
| PstNI | CAGNNNCTG | 1 cut(s) | 659 |
| PsuI | RGATCY | 6 cut(s) | 532, 766, 2017, 2198, 2899, 3103 |
| RsaI | GTAC | 7 cut(s) | 36, 384, 810, 1313, 2149, 2345, 2631 |
| RsaNI | GTAC | 7 cut(s) | 35, 383, 809, 1312, 2148, 2344, 2630 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1344, 2106 |
| Sau96I | GGNCC | 6 cut(s) | 129, 602, 1649, 2141, 2537, 2603 |
| SchI | GAGTC | 1 cut(s) | 2711 |
| ScrFI | CCNGG | 8 cut(s) | 744, 752, 1524, 1672, 1925, 2333, 2415, 2837 |
| SduI | GDGCHC | 5 cut(s) | 1642, 2464, 2819, 2835, 3033 |
| SfaNI | GCATC | 6 cut(s) | 74, 227, 646, 692, 967, 1210 |
| SfcI | CTRYAG | 1 cut(s) | 703 |
| Sfr274I | CTCGAG | 1 cut(s) | 546 |
| SfuI | TTCGAA | 2 cut(s) | 90, 2595 |
| SinI | GGWCC | 5 cut(s) | 602, 1649, 2141, 2537, 2603 |
| SlaI | CTCGAG | 1 cut(s) | 546 |
| SmiI | ATTTAAAT | 1 cut(s) | 2040 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1344, 2106 |
| SmlI | CTYRAG | 6 cut(s) | 546, 919, 1872, 1946, 2980, 3219 |
| SmoI | CTYRAG | 6 cut(s) | 546, 919, 1872, 1946, 2980, 3219 |
| SphI | GCATGC | 2 cut(s) | 837, 2157 |
| SseBI | AGGCCT | 1 cut(s) | 991 |
| SsiI | CCGC | 8 cut(s) | 32, 62, 127, 202, 1471, 1610, 1652, 2447 |
| SspI | AATATT | 1 cut(s) | 2686 |
| SspMI | CTAG | 8 cut(s) | 455, 536, 761, 936, 1242, 1454, 2202, 2360 |
| StuI | AGGCCT | 1 cut(s) | 991 |
| StyD4I | CCNGG | 8 cut(s) | 742, 750, 1522, 1670, 1923, 2331, 2413, 2835 |
| StyI | CCWWGG | 2 cut(s) | 1040, 2780 |
| SwaI | ATTTAAAT | 1 cut(s) | 2040 |
| TaaI | ACNGT | 9 cut(s) | 192, 1176, 1307, 1316, 1373, 1483, 1845, 2327, 2455 |
| TaiI | ACGT | 4 cut(s) | 417, 886, 1189, 1803 |
| TaqI | TCGA | 5 cut(s) | 90, 547, 575, 2482, 2595 |
| TatI | WGTACW | 4 cut(s) | 382, 808, 2343, 2629 |
| TauI | GCSGC | 5 cut(s) | 65, 130, 205, 1473, 2450 |
| TfiI | GAWTC | 6 cut(s) | 511, 662, 1550, 2278, 2759, 2969 |
| TscAI | CASTG | 5 cut(s) | 784, 1156, 2192, 2875, 3192 |
| TseFI | GTSAC | 3 cut(s) | 1398, 1499, 1661 |
| TseI | GCWGC | 9 cut(s) | 205, 428, 463, 965, 1094, 1127, 1951, 2117, 2987 |
| Tsp45I | GTSAC | 3 cut(s) | 1398, 1499, 1661 |
| TspGWI | ACGGA | 2 cut(s) | 408, 434 |
| TspRI | CASTG | 5 cut(s) | 784, 1156, 2192, 2875, 3192 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 2493 |
| Vha464I | CTTAAG | 1 cut(s) | 919 |
| VpaK11BI | GGWCC | 5 cut(s) | 602, 1649, 2141, 2537, 2603 |
| VspI | ATTAAT | 1 cut(s) | 972 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 996 |
| XapI | RAATTY | 7 cut(s) | 541, 560, 1561, 2035, 2795, 2926, 3203 |
| XceI | RCATGY | 3 cut(s) | 837, 2157, 3128 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 2192, 3043 |
| XhoI | CTCGAG | 1 cut(s) | 546 |
| XmiI | GTMKAC | 1 cut(s) | 1905 |
| XmnI | GAANNNNTTC | 2 cut(s) | 306, 2282 |
| XspI | CTAG | 8 cut(s) | 455, 536, 761, 936, 1242, 1454, 2202, 2360 |
| ZraI | GACGTC | 1 cut(s) | 1187 |
| Zsp2I | ATGCAT | 4 cut(s) | 835, 1225, 2472, 3005 |