phospholipase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 2486, 2988 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 2972 |
| Acc36I | ACCTGC | 2 cut(s) | 316, 1215 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 626, 1822 |
| AccBSI | CCGCTC | 2 cut(s) | 113, 898 |
| AccI | GTMKAC | 1 cut(s) | 2824 |
| AccII | CGCG | 1 cut(s) | 3305 |
| AccIII | TCCGGA | 1 cut(s) | 2040 |
| AciI | CCGC | 9 cut(s) | 30, 37, 111, 898, 1045, 1210, 1775, 2498, 2694 |
| AclWI | GGATC | 5 cut(s) | 27, 40, 1629, 2159, 2776 |
| AcoI | YGGCCR | 3 cut(s) | 63, 473, 865 |
| AcsI | RAATTY | 5 cut(s) | 306, 675, 1588, 2052, 2622 |
| AcyI | GRCGYC | 2 cut(s) | 168, 1213 |
| AfaI | GTAC | 4 cut(s) | 200, 837, 1205, 1383 |
| AfeI | AGCGCT | 4 cut(s) | 427, 454, 480, 1715 |
| AflIII | ACRYGT | 2 cut(s) | 256, 3303 |
| AjnI | CCWGG | 6 cut(s) | 591, 695, 769, 1549, 2514, 3074 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1601, 1633, 2962, 2994 |
| AleI | CACNNNNGTG | 1 cut(s) | 371 |
| AluBI | AGCT | 7 cut(s) | 637, 1133, 1268, 1313, 1931, 2555, 2774 |
| AluI | AGCT | 7 cut(s) | 637, 1133, 1268, 1313, 1931, 2555, 2774 |
| Alw21I | GWGCWC | 1 cut(s) | 2355 |
| Alw26I | GTCTC | 7 cut(s) | 98, 154, 160, 392, 604, 2118, 3312 |
| AlwI | GGATC | 5 cut(s) | 27, 40, 1629, 2159, 2776 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2546 |
| Ama87I | CYCGRG | 3 cut(s) | 573, 1358, 3152 |
| Aor13HI | TCCGGA | 1 cut(s) | 2040 |
| Aor51HI | AGCGCT | 4 cut(s) | 427, 454, 480, 1715 |
| ApeKI | GCWGC | 5 cut(s) | 412, 455, 1777, 2540, 3080 |
| ApoI | RAATTY | 5 cut(s) | 306, 675, 1588, 2052, 2622 |
| AseI | ATTAAT | 2 cut(s) | 948, 2850 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 339, 1278, 2890 |
| AspLEI | GCGC | 6 cut(s) | 140, 428, 455, 481, 793, 1716 |
| AspS9I | GGNCC | 7 cut(s) | 629, 1425, 1676, 2066, 2234, 2696, 3226 |
| AsuC2I | CCSGG | 3 cut(s) | 25, 869, 2508 |
| AsuHPI | GGTGA | 2 cut(s) | 1034, 1538 |
| AvaI | CYCGRG | 3 cut(s) | 573, 1358, 3152 |
| AvaII | GGWCC | 4 cut(s) | 1425, 1676, 2066, 2696 |
| BaeGI | GKGCMC | 2 cut(s) | 1669, 3126 |
| BamHI | GGATCC | 1 cut(s) | 32 |
| BanII | GRGCYC | 1 cut(s) | 111 |
| BauI | CACGAG | 1 cut(s) | 2245 |
| BbsI | GAAGAC | 3 cut(s) | 651, 2894, 3194 |
| Bbv12I | GWGCWC | 1 cut(s) | 2355 |
| BbvI | GCAGC | 5 cut(s) | 424, 442, 1789, 2552, 3067 |
| BceAI | ACGGC | 4 cut(s) | 75, 926, 1671, 2427 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 564, 598, 2565, 2599 |
| BciT130I | CCWGG | 6 cut(s) | 593, 697, 771, 1551, 2516, 3076 |
| BciVI | GTATCC | 1 cut(s) | 1594 |
| BclI | TGATCA | 1 cut(s) | 1999 |
| BcnI | CCSGG | 3 cut(s) | 25, 869, 2508 |
| BcoDI | GTCTC | 7 cut(s) | 98, 154, 160, 392, 604, 2118, 3312 |
| BfaI | CTAG | 6 cut(s) | 350, 450, 548, 1239, 1698, 2102 |
| BfmI | CTRYAG | 2 cut(s) | 410, 2707 |
| BfoI | RGCGCY | 5 cut(s) | 141, 429, 456, 482, 1717 |
| BfuAI | ACCTGC | 2 cut(s) | 316, 1215 |
| BfuI | GTATCC | 1 cut(s) | 1594 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 788 |
| BglII | AGATCT | 4 cut(s) | 509, 2669, 2715, 3268 |
| BisI | GCNGC | 7 cut(s) | 413, 456, 1211, 1775, 1778, 2541, 3081 |
| BlpI | GCTNAGC | 1 cut(s) | 1512 |
| BlsI | GCNGC | 7 cut(s) | 414, 457, 1212, 1776, 1779, 2542, 3082 |
| Bme1390I | CCNGG | 9 cut(s) | 25, 593, 697, 771, 869, 1551, 2508, 2516, 3076 |
| Bme18I | GGWCC | 4 cut(s) | 1425, 1676, 2066, 2696 |
| BmeT110I | CYCGRG | 3 cut(s) | 573, 1358, 3152 |
| BmgT120I | GGNCC | 7 cut(s) | 629, 1425, 1676, 2066, 2234, 2696, 3226 |
| BmiI | GGNNCC | 5 cut(s) | 34, 970, 1427, 1677, 1743 |
| BmrFI | CCNGG | 9 cut(s) | 25, 593, 697, 771, 869, 1551, 2508, 2516, 3076 |
| BmsI | GCATC | 7 cut(s) | 673, 802, 994, 1144, 2521, 2552, 2628 |
| BpiI | GAAGAC | 3 cut(s) | 651, 2894, 3194 |
| BpmI | CTGGAG | 3 cut(s) | 603, 614, 1005 |
| Bpu10I | CCTNAGC | 2 cut(s) | 633, 1269 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1512 |
| BpuEI | CTTGAG | 2 cut(s) | 1390, 3297 |
| BpuMI | CCSGG | 3 cut(s) | 25, 869, 2508 |
| BsaHI | GRCGYC | 2 cut(s) | 168, 1213 |
| BsaI | GGTCTC | 1 cut(s) | 604 |
| BsaJI | CCNNGG | 8 cut(s) | 24, 1571, 1646, 2515, 2873, 2929, 3075, 3201 |
| BsaWI | WCCGGW | 1 cut(s) | 2040 |
| Bse118I | RCCGGY | 1 cut(s) | 170 |
| Bse1I | ACTGG | 4 cut(s) | 882, 1260, 1485, 2060 |
| Bse3DI | GCAATG | 2 cut(s) | 528, 862 |
| BseAI | TCCGGA | 1 cut(s) | 2040 |
| BseBI | CCWGG | 6 cut(s) | 593, 697, 771, 1551, 2516, 3076 |
| BseDI | CCNNGG | 8 cut(s) | 24, 1571, 1646, 2515, 2873, 2929, 3075, 3201 |
| BseMI | GCAATG | 2 cut(s) | 528, 862 |
| BseMII | CTCAG | 3 cut(s) | 861, 1503, 2805 |
| BseNI | ACTGG | 4 cut(s) | 882, 1260, 1485, 2060 |
| BseRI | GAGGAG | 2 cut(s) | 67, 3300 |
| BseSI | GKGCMC | 2 cut(s) | 1669, 3126 |
| BseXI | GCAGC | 5 cut(s) | 424, 442, 1789, 2552, 3067 |
| BseYI | CCCAGC | 2 cut(s) | 134, 481 |
| BsgI | GTGCAG | 1 cut(s) | 2559 |
| Bsh1236I | CGCG | 1 cut(s) | 3305 |
| Bsh1285I | CGRYCG | 1 cut(s) | 56 |
| BsiEI | CGRYCG | 1 cut(s) | 56 |
| BsiHKAI | GWGCWC | 1 cut(s) | 2355 |
| BsiHKCI | CYCGRG | 3 cut(s) | 573, 1358, 3152 |
| BsiSI | CCGG | 7 cut(s) | 24, 171, 472, 868, 940, 2041, 2508 |
| BslFI | GGGAC | 4 cut(s) | 147, 1411, 1689, 2238 |
| BsmAI | GTCTC | 7 cut(s) | 98, 154, 160, 392, 604, 2118, 3312 |
| BsmBI | CGTCTC | 4 cut(s) | 98, 154, 160, 3312 |
| BsmFI | GGGAC | 4 cut(s) | 147, 1411, 1689, 2238 |
| BsmI | GAATGC | 2 cut(s) | 2313, 3083 |
| Bso31I | GGTCTC | 1 cut(s) | 604 |
| BsoBI | CYCGRG | 3 cut(s) | 573, 1358, 3152 |
| Bsp1286I | GDGCHC | 4 cut(s) | 111, 1669, 2355, 3126 |
| Bsp13I | TCCGGA | 1 cut(s) | 2040 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1512 |
| Bsp19I | CCATGG | 2 cut(s) | 2873, 3201 |
| BspACI | CCGC | 9 cut(s) | 30, 37, 111, 898, 1045, 1210, 1775, 2498, 2694 |
| BspCNI | CTCAG | 3 cut(s) | 860, 1504, 2806 |
| BspEI | TCCGGA | 1 cut(s) | 2040 |
| BspFNI | CGCG | 1 cut(s) | 3305 |
| BspHI | TCATGA | 1 cut(s) | 1366 |
| BspLI | GGNNCC | 5 cut(s) | 34, 970, 1427, 1677, 1743 |
| BspMAI | CTGCAG | 1 cut(s) | 414 |
| BspMI | ACCTGC | 2 cut(s) | 316, 1215 |
| BspPI | GGATC | 5 cut(s) | 27, 40, 1629, 2159, 2776 |
| BspTNI | GGTCTC | 1 cut(s) | 604 |
| BsrBI | CCGCTC | 2 cut(s) | 113, 898 |
| BsrDI | GCAATG | 2 cut(s) | 528, 862 |
| BsrFI | RCCGGY | 1 cut(s) | 170 |
| BsrI | ACTGG | 4 cut(s) | 882, 1260, 1485, 2060 |
| BssAI | RCCGGY | 1 cut(s) | 170 |
| BssECI | CCNNGG | 8 cut(s) | 24, 1571, 1646, 2515, 2873, 2929, 3075, 3201 |
| BssNI | GRCGYC | 2 cut(s) | 168, 1213 |
| BssSI | CACGAG | 1 cut(s) | 2245 |
| BssT1I | CCWWGG | 4 cut(s) | 1571, 1646, 2873, 3201 |
| Bst2BI | CACGAG | 1 cut(s) | 2245 |
| Bst2UI | CCWGG | 6 cut(s) | 593, 697, 771, 1551, 2516, 3076 |
| Bst4CI | ACNGT | 6 cut(s) | 403, 1203, 1334, 1664, 2151, 2916 |
| Bst6I | CTCTTC | 1 cut(s) | 2301 |
| BstACI | GRCGYC | 2 cut(s) | 168, 1213 |
| BstC8I | GCNNGC | 6 cut(s) | 111, 172, 291, 477, 789, 2454 |
| BstDEI | CTNAG | 8 cut(s) | 513, 633, 847, 1013, 1269, 1512, 2666, 2814 |
| BstDSI | CCRYGG | 2 cut(s) | 2873, 3201 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1068 |
| BstFNI | CGCG | 1 cut(s) | 3305 |
| BstH2I | RGCGCY | 5 cut(s) | 141, 429, 456, 482, 1717 |
| BstHHI | GCGC | 6 cut(s) | 140, 428, 455, 481, 793, 1716 |
| BstMAI | GTCTC | 7 cut(s) | 98, 154, 160, 392, 604, 2118, 3312 |
| BstMCI | CGRYCG | 1 cut(s) | 56 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 36, 180, 788, 1130, 2016, 2241, 2540 |
| BstNI | CCWGG | 6 cut(s) | 593, 697, 771, 1551, 2516, 3076 |
| BstNSI | RCATGY | 3 cut(s) | 260, 714, 2911 |
| BstSCI | CCNGG | 9 cut(s) | 23, 591, 695, 769, 867, 1549, 2506, 2514, 3074 |
| BstSFI | CTRYAG | 2 cut(s) | 410, 2707 |
| BstSLI | GKGCMC | 2 cut(s) | 1669, 3126 |
| BstUI | CGCG | 1 cut(s) | 3305 |
| BstV1I | GCAGC | 5 cut(s) | 424, 442, 1789, 2552, 3067 |
| BstV2I | GAAGAC | 3 cut(s) | 651, 2894, 3194 |
| BstX2I | RGATCY | 6 cut(s) | 32, 509, 2164, 2669, 2715, 3268 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 1029, 1593, 2175 |
| BstYI | RGATCY | 6 cut(s) | 32, 509, 2164, 2669, 2715, 3268 |
| BsuI | GTATCC | 1 cut(s) | 1594 |
| BtgI | CCRYGG | 2 cut(s) | 2873, 3201 |
| BtsIMutI | CAGTG | 6 cut(s) | 366, 875, 1339, 1660, 2285, 2912 |
| BveI | ACCTGC | 2 cut(s) | 316, 1215 |
| Cac8I | GCNNGC | 6 cut(s) | 111, 172, 291, 477, 789, 2454 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2546 |
| CciI | TCATGA | 1 cut(s) | 1366 |
| CfoI | GCGC | 6 cut(s) | 140, 428, 455, 481, 793, 1716 |
| Cfr10I | RCCGGY | 1 cut(s) | 170 |
| Cfr13I | GGNCC | 7 cut(s) | 629, 1425, 1676, 2066, 2234, 2696, 3226 |
| CpoI | CGGWCCG | 1 cut(s) | 2696 |
| CseI | GACGC | 4 cut(s) | 176, 1202, 2260, 3294 |
| Csp6I | GTAC | 4 cut(s) | 199, 836, 1204, 1382 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 2185, 2220 |
| CspI | CGGWCCG | 1 cut(s) | 2696 |
| CviQI | GTAC | 4 cut(s) | 199, 836, 1204, 1382 |
| DdeI | CTNAG | 8 cut(s) | 513, 633, 847, 1013, 1269, 1512, 2666, 2814 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 2972 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 2972 |
| EaeI | YGGCCR | 3 cut(s) | 63, 473, 865 |
| Eam1104I | CTCTTC | 1 cut(s) | 2301 |
| EarI | CTCTTC | 1 cut(s) | 2301 |
| EciI | GGCGGA | 1 cut(s) | 45 |
| Eco130I | CCWWGG | 4 cut(s) | 1571, 1646, 2873, 3201 |
| Eco147I | AGGCCT | 1 cut(s) | 1018 |
| Eco24I | GRGCYC | 1 cut(s) | 111 |
| Eco31I | GGTCTC | 1 cut(s) | 604 |
| Eco32I | GATATC | 2 cut(s) | 178, 1890 |
| Eco47I | GGWCC | 4 cut(s) | 1425, 1676, 2066, 2696 |
| Eco47III | AGCGCT | 4 cut(s) | 427, 454, 480, 1715 |
| Eco88I | CYCGRG | 3 cut(s) | 573, 1358, 3152 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1068 |
| EcoO109I | RGGNCCY | 1 cut(s) | 2066 |
| EcoRII | CCWGG | 6 cut(s) | 591, 695, 769, 1549, 2514, 3074 |
| EcoRV | GATATC | 2 cut(s) | 178, 1890 |
| EcoT14I | CCWWGG | 4 cut(s) | 1571, 1646, 2873, 3201 |
| EcoT22I | ATGCAT | 2 cut(s) | 2565, 2643 |
| EcoT38I | GRGCYC | 1 cut(s) | 111 |
| ErhI | CCWWGG | 4 cut(s) | 1571, 1646, 2873, 3201 |
| Esp3I | CGTCTC | 4 cut(s) | 98, 154, 160, 3312 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 324, 356 |
| FaqI | GGGAC | 4 cut(s) | 147, 1411, 1689, 2238 |
| FauI | CCCGC | 2 cut(s) | 44, 118 |
| FbaI | TGATCA | 1 cut(s) | 1999 |
| FblI | GTMKAC | 1 cut(s) | 2824 |
| Fnu4HI | GCNGC | 7 cut(s) | 413, 456, 1211, 1775, 1778, 2541, 3081 |
| FriOI | GRGCYC | 1 cut(s) | 111 |
| Fsp4HI | GCNGC | 7 cut(s) | 413, 456, 1211, 1775, 1778, 2541, 3081 |
| FspBI | CTAG | 6 cut(s) | 350, 450, 548, 1239, 1698, 2102 |
| GlaI | GCGC | 6 cut(s) | 139, 427, 454, 480, 792, 1715 |
| GluI | GCNGC | 7 cut(s) | 413, 456, 1211, 1775, 1778, 2541, 3081 |
| GsaI | CCCAGC | 2 cut(s) | 138, 485 |
| GsuI | CTGGAG | 3 cut(s) | 603, 614, 1005 |
| HaeII | RGCGCY | 5 cut(s) | 141, 429, 456, 482, 1717 |
| HapII | CCGG | 7 cut(s) | 24, 171, 472, 868, 940, 2041, 2508 |
| HgaI | GACGC | 4 cut(s) | 176, 1202, 2260, 3294 |
| HhaI | GCGC | 6 cut(s) | 140, 428, 455, 481, 793, 1716 |
| Hin1I | GRCGYC | 2 cut(s) | 168, 1213 |
| Hin6I | GCGC | 6 cut(s) | 138, 426, 453, 479, 791, 1714 |
| HinP1I | GCGC | 6 cut(s) | 138, 426, 453, 479, 791, 1714 |
| HpaII | CCGG | 7 cut(s) | 24, 171, 472, 868, 940, 2041, 2508 |
| HphI | GGTGA | 2 cut(s) | 1034, 1538 |
| Hpy166II | GTNNAC | 6 cut(s) | 201, 568, 1417, 1660, 2681, 2825 |
| Hpy8I | GTNNAC | 6 cut(s) | 201, 568, 1417, 1660, 2681, 2825 |
| Hpy99I | CGWCG | 3 cut(s) | 128, 170, 1888 |
| HpyCH4III | ACNGT | 6 cut(s) | 403, 1203, 1334, 1664, 2151, 2916 |
| HpyCH4IV | ACGT | 2 cut(s) | 1387, 1656 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 36, 180, 788, 1130, 2016, 2241, 2540 |
| HpyF3I | CTNAG | 8 cut(s) | 513, 633, 847, 1013, 1269, 1512, 2666, 2814 |
| HpySE526I | ACGT | 2 cut(s) | 1387, 1656 |
| Hsp92I | GRCGYC | 2 cut(s) | 168, 1213 |
| HspAI | GCGC | 6 cut(s) | 138, 426, 453, 479, 791, 1714 |
| Kpn2I | TCCGGA | 1 cut(s) | 2040 |
| KroI | GCCGGC | 1 cut(s) | 170 |
| KroNI | GCCGGC | 1 cut(s) | 172 |
| Ksp22I | TGATCA | 1 cut(s) | 1999 |
| LmnI | GCTCC | 5 cut(s) | 10, 490, 942, 1747, 3154 |
| Lsp1109I | GCAGC | 5 cut(s) | 424, 442, 1789, 2552, 3067 |
| LweI | GCATC | 7 cut(s) | 673, 802, 994, 1144, 2521, 2552, 2628 |
| MaeI | CTAG | 6 cut(s) | 350, 450, 548, 1239, 1698, 2102 |
| MaeII | ACGT | 2 cut(s) | 1387, 1656 |
| MaeIII | GTNAC | 2 cut(s) | 1499, 2910 |
| MbiI | CCGCTC | 2 cut(s) | 113, 898 |
| MfeI | CAATTG | 4 cut(s) | 724, 739, 1146, 2970 |
| MflI | RGATCY | 6 cut(s) | 32, 509, 2164, 2669, 2715, 3268 |
| MhlI | GDGCHC | 4 cut(s) | 111, 1669, 2355, 3126 |
| MluI | ACGCGT | 1 cut(s) | 3303 |
| MlyI | GAGTC | 3 cut(s) | 68, 1973, 2908 |
| MmeI | TCCRAC | 1 cut(s) | 1599 |
| Mph1103I | ATGCAT | 2 cut(s) | 2565, 2643 |
| MreI | CGCCGGCG | 1 cut(s) | 170 |
| MroI | TCCGGA | 1 cut(s) | 2040 |
| MroNI | GCCGGC | 1 cut(s) | 170 |
| MroXI | GAANNNNTTC | 3 cut(s) | 339, 1278, 2890 |
| MslI | CAYNNNNRTG | 6 cut(s) | 369, 371, 1227, 1371, 2948, 2999 |
| MspI | CCGG | 7 cut(s) | 24, 171, 472, 868, 940, 2041, 2508 |
| MspR9I | CCNGG | 9 cut(s) | 25, 593, 697, 771, 869, 1551, 2508, 2516, 3076 |
| MunI | CAATTG | 4 cut(s) | 724, 739, 1146, 2970 |
| Mva1269I | GAATGC | 2 cut(s) | 2313, 3083 |
| MvaI | CCWGG | 6 cut(s) | 593, 697, 771, 1551, 2516, 3076 |
| MvnI | CGCG | 1 cut(s) | 3305 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 36, 180, 788, 1130, 2016, 2241, 2540 |
| NaeI | GCCGGC | 1 cut(s) | 172 |
| NciI | CCSGG | 3 cut(s) | 25, 869, 2508 |
| NcoI | CCATGG | 2 cut(s) | 2873, 3201 |
| NgoMIV | GCCGGC | 1 cut(s) | 170 |
| NlaIV | GGNNCC | 5 cut(s) | 34, 970, 1427, 1677, 1743 |
| NmuCI | GTSAC | 1 cut(s) | 2910 |
| NsiI | ATGCAT | 2 cut(s) | 2565, 2643 |
| NspI | RCATGY | 3 cut(s) | 260, 714, 2911 |
| OliI | CACNNNNGTG | 1 cut(s) | 371 |
| PaeR7I | CTCGAG | 1 cut(s) | 573 |
| PagI | TCATGA | 1 cut(s) | 1366 |
| PceI | AGGCCT | 1 cut(s) | 1018 |
| PciI | ACATGT | 1 cut(s) | 256 |
| PctI | GAATGC | 2 cut(s) | 2313, 3083 |
| PdiI | GCCGGC | 1 cut(s) | 172 |
| PdmI | GAANNNNTTC | 3 cut(s) | 339, 1278, 2890 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 626, 1822 |
| PfoI | TCCNGGA | 1 cut(s) | 591 |
| PkrI | GCNGC | 7 cut(s) | 414, 457, 1212, 1776, 1779, 2542, 3082 |
| Ple19I | CGATCG | 1 cut(s) | 56 |
| PleI | GAGTC | 3 cut(s) | 67, 1973, 2907 |
| PpsI | GAGTC | 3 cut(s) | 67, 1973, 2907 |
| PpuMI | RGGWCCY | 1 cut(s) | 2066 |
| PscI | ACATGT | 1 cut(s) | 256 |
| PshBI | ATTAAT | 2 cut(s) | 948, 2850 |
| PsiI | TTATAA | 2 cut(s) | 2486, 2988 |
| Psp5II | RGGWCCY | 1 cut(s) | 2066 |
| Psp6I | CCWGG | 6 cut(s) | 591, 695, 769, 1549, 2514, 3074 |
| PspFI | CCCAGC | 2 cut(s) | 134, 481 |
| PspGI | CCWGG | 6 cut(s) | 591, 695, 769, 1549, 2514, 3074 |
| PspN4I | GGNNCC | 5 cut(s) | 34, 970, 1427, 1677, 1743 |
| PspPI | GGNCC | 7 cut(s) | 629, 1425, 1676, 2066, 2234, 2696, 3226 |
| PspPPI | RGGWCCY | 1 cut(s) | 2066 |
| PstI | CTGCAG | 1 cut(s) | 414 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2546 |
| PsuI | RGATCY | 6 cut(s) | 32, 509, 2164, 2669, 2715, 3268 |
| PvuI | CGATCG | 1 cut(s) | 56 |
| RsaI | GTAC | 4 cut(s) | 200, 837, 1205, 1383 |
| RsaNI | GTAC | 4 cut(s) | 199, 836, 1204, 1382 |
| RseI | CAYNNNNRTG | 6 cut(s) | 369, 371, 1227, 1371, 2948, 2999 |
| Rsr2I | CGGWCCG | 1 cut(s) | 2696 |
| RsrII | CGGWCCG | 1 cut(s) | 2696 |
| SatI | GCNGC | 7 cut(s) | 413, 456, 1211, 1775, 1778, 2541, 3081 |
| Sau96I | GGNCC | 7 cut(s) | 629, 1425, 1676, 2066, 2234, 2696, 3226 |
| SchI | GAGTC | 3 cut(s) | 68, 1973, 2908 |
| ScrFI | CCNGG | 9 cut(s) | 25, 593, 697, 771, 869, 1551, 2508, 2516, 3076 |
| SduI | GDGCHC | 4 cut(s) | 111, 1669, 2355, 3126 |
| SfaNI | GCATC | 7 cut(s) | 673, 802, 994, 1144, 2521, 2552, 2628 |
| SfcI | CTRYAG | 2 cut(s) | 410, 2707 |
| Sfr274I | CTCGAG | 1 cut(s) | 573 |
| SgrAI | CRCCGGYG | 1 cut(s) | 170 |
| SinI | GGWCC | 4 cut(s) | 1425, 1676, 2066, 2696 |
| SlaI | CTCGAG | 1 cut(s) | 573 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 369, 371, 1227, 1371, 2948, 2999 |
| SmlI | CTYRAG | 3 cut(s) | 573, 1405, 3312 |
| SmoI | CTYRAG | 3 cut(s) | 573, 1405, 3312 |
| SseBI | AGGCCT | 1 cut(s) | 1018 |
| SsiI | CCGC | 9 cut(s) | 30, 37, 111, 898, 1045, 1210, 1775, 2498, 2694 |
| SspI | AATATT | 1 cut(s) | 2604 |
| SspMI | CTAG | 6 cut(s) | 350, 450, 548, 1239, 1698, 2102 |
| StuI | AGGCCT | 1 cut(s) | 1018 |
| StyD4I | CCNGG | 9 cut(s) | 23, 591, 695, 769, 867, 1549, 2506, 2514, 3074 |
| StyI | CCWWGG | 4 cut(s) | 1571, 1646, 2873, 3201 |
| TaaI | ACNGT | 6 cut(s) | 403, 1203, 1334, 1664, 2151, 2916 |
| TaiI | ACGT | 2 cut(s) | 1390, 1659 |
| TatI | WGTACW | 1 cut(s) | 835 |
| TauI | GCSGC | 2 cut(s) | 1213, 1777 |
| TscAI | CASTG | 6 cut(s) | 373, 882, 1339, 1667, 2285, 2919 |
| TseFI | GTSAC | 1 cut(s) | 2910 |
| TseI | GCWGC | 5 cut(s) | 412, 455, 1777, 2540, 3080 |
| Tsp45I | GTSAC | 1 cut(s) | 2910 |
| TspGWI | ACGGA | 5 cut(s) | 142, 1886, 2136, 2428, 2688 |
| TspRI | CASTG | 6 cut(s) | 373, 882, 1339, 1667, 2285, 2919 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 626, 1822 |
| VpaK11BI | GGWCC | 4 cut(s) | 1425, 1676, 2066, 2696 |
| VspI | ATTAAT | 2 cut(s) | 948, 2850 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1068 |
| XapI | RAATTY | 5 cut(s) | 306, 675, 1588, 2052, 2622 |
| XbaI | TCTAGA | 1 cut(s) | 1238 |
| XceI | RCATGY | 3 cut(s) | 260, 714, 2911 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 1406, 1931 |
| XhoI | CTCGAG | 1 cut(s) | 573 |
| XmiI | GTMKAC | 1 cut(s) | 2824 |
| XmnI | GAANNNNTTC | 3 cut(s) | 339, 1278, 2890 |
| XspI | CTAG | 6 cut(s) | 350, 450, 548, 1239, 1698, 2102 |
| Zsp2I | ATGCAT | 2 cut(s) | 2565, 2643 |