phospholipase D
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1530 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 1647, 2202 |
| AatII | GACGTC | 1 cut(s) | 1180 |
| Acc36I | ACCTGC | 1 cut(s) | 274 |
| AccB1I | GGYRCC | 3 cut(s) | 29, 70, 996 |
| AccB7I | CCANNNNNTGG | 3 cut(s) | 359, 511, 2556 |
| AccII | CGCG | 2 cut(s) | 118, 1603 |
| AciI | CCGC | 6 cut(s) | 60, 193, 317, 420, 1601, 1678 |
| AcoI | YGGCCR | 4 cut(s) | 96, 1459, 1511, 2832 |
| AcsI | RAATTY | 7 cut(s) | 551, 638, 1377, 1552, 2813, 2989, 3266 |
| AcuI | CTGAAG | 2 cut(s) | 221, 2295 |
| AcyI | GRCGYC | 1 cut(s) | 1177 |
| AdeI | CACNNNGTG | 2 cut(s) | 1486, 2329 |
| AfaI | GTAC | 5 cut(s) | 36, 176, 2212, 2408, 2694 |
| AflII | CTTAAG | 1 cut(s) | 910 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 494, 526, 1565, 1597 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1832, 1864 |
| Alw21I | GWGCWC | 2 cut(s) | 1633, 2882 |
| Alw26I | GTCTC | 4 cut(s) | 469, 1808, 2772, 3238 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2516 |
| AoxI | GGCC | 9 cut(s) | 96, 744, 973, 980, 1459, 1511, 2112, 2665, 2832 |
| ApeKI | GCWGC | 5 cut(s) | 454, 1085, 2180, 2510, 3050 |
| ApoI | RAATTY | 7 cut(s) | 551, 638, 1377, 1552, 2813, 2989, 3266 |
| AseI | ATTAAT | 2 cut(s) | 963, 2751 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1596, 2860 |
| AspLEI | GCGC | 1 cut(s) | 1353 |
| AspS9I | GGNCC | 6 cut(s) | 593, 1640, 2113, 2204, 2600, 2666 |
| AsuHPI | GGTGA | 3 cut(s) | 634, 1091, 1502 |
| AsuNHI | GCTAGC | 1 cut(s) | 2971 |
| AvaII | GGWCC | 4 cut(s) | 593, 1640, 2204, 2600 |
| AxyI | CCTNAGG | 1 cut(s) | 977 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 90, 123 |
| BalI | TGGCCA | 2 cut(s) | 98, 1513 |
| BamHI | GGATCC | 1 cut(s) | 1827 |
| BanI | GGYRCC | 3 cut(s) | 29, 70, 996 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 1180, 1212, 2268, 2300 |
| BauI | CACGAG | 1 cut(s) | 414 |
| BbsI | GAAGAC | 1 cut(s) | 2897 |
| Bbv12I | GWGCWC | 2 cut(s) | 1633, 2882 |
| BbvI | GCAGC | 5 cut(s) | 466, 1097, 2167, 2522, 3037 |
| BceAI | ACGGC | 2 cut(s) | 17, 1446 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 528, 562, 2535, 2569 |
| BciVI | GTATCC | 1 cut(s) | 2402 |
| BclI | TGATCA | 1 cut(s) | 2167 |
| BcoDI | GTCTC | 4 cut(s) | 469, 1808, 2772, 3238 |
| BfrI | CTTAAG | 1 cut(s) | 910 |
| BfuAI | ACCTGC | 1 cut(s) | 274 |
| BfuI | GTATCC | 1 cut(s) | 2402 |
| BglII | AGATCT | 1 cut(s) | 2962 |
| BisI | GCNGC | 6 cut(s) | 420, 455, 1086, 2181, 2511, 3051 |
| BlpI | GCTNAGC | 1 cut(s) | 1233 |
| BlsI | GCNGC | 6 cut(s) | 421, 456, 1087, 2182, 2512, 3052 |
| Bme18I | GGWCC | 4 cut(s) | 593, 1640, 2204, 2600 |
| BmgT120I | GGNCC | 6 cut(s) | 593, 1640, 2113, 2204, 2600, 2666 |
| BmsI | GCATC | 7 cut(s) | 218, 637, 674, 958, 1201, 1938, 1960 |
| BmtI | GCTAGC | 1 cut(s) | 2975 |
| BpiI | GAAGAC | 1 cut(s) | 2897 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 1045, 1077, 3039, 3071 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1233 |
| BpuEI | CTTGAG | 3 cut(s) | 1875, 3028, 3267 |
| Bsa29I | ATCGAT | 1 cut(s) | 3105 |
| BsaHI | GRCGYC | 1 cut(s) | 1177 |
| BsaI | GGTCTC | 2 cut(s) | 1808, 3238 |
| BsaJI | CCNNGG | 9 cut(s) | 734, 1031, 1032, 1514, 1610, 2829, 2843, 2899, 3138 |
| BsaWI | WCCGGW | 1 cut(s) | 128 |
| Bse118I | RCCGGY | 1 cut(s) | 91 |
| Bse1I | ACTGG | 4 cut(s) | 756, 1147, 2248, 3248 |
| Bse21I | CCTNAGG | 1 cut(s) | 977 |
| Bse3DI | GCAATG | 4 cut(s) | 62, 492, 937, 1656 |
| BseCI | ATCGAT | 1 cut(s) | 3105 |
| BseDI | CCNNGG | 9 cut(s) | 734, 1031, 1032, 1514, 1610, 2829, 2843, 2899, 3138 |
| BseGI | GGATG | 6 cut(s) | 432, 760, 1000, 1755, 2068, 2503 |
| BseMI | GCAATG | 4 cut(s) | 62, 492, 937, 1656 |
| BseMII | CTCAG | 5 cut(s) | 480, 1076, 2627, 2775, 3070 |
| BseNI | ACTGG | 4 cut(s) | 756, 1147, 2248, 3248 |
| BseRI | GAGGAG | 3 cut(s) | 275, 1017, 3270 |
| BseXI | GCAGC | 5 cut(s) | 466, 1097, 2167, 2522, 3037 |
| BseYI | CCCAGC | 1 cut(s) | 2556 |
| BsgI | GTGCAG | 1 cut(s) | 2529 |
| Bsh1236I | CGCG | 2 cut(s) | 118, 1603 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1363 |
| BshFI | GGCC | 9 cut(s) | 98, 746, 975, 982, 1461, 1513, 2114, 2667, 2834 |
| BshNI | GGYRCC | 3 cut(s) | 29, 70, 996 |
| BshVI | ATCGAT | 1 cut(s) | 3105 |
| BsiEI | CGRYCG | 1 cut(s) | 1363 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1633, 2882 |
| BsiSI | CCGG | 4 cut(s) | 52, 92, 129, 2835 |
| BslFI | GGGAC | 3 cut(s) | 105, 1653, 3210 |
| BsmAI | GTCTC | 4 cut(s) | 469, 1808, 2772, 3238 |
| BsmBI | CGTCTC | 1 cut(s) | 469 |
| BsmFI | GGGAC | 3 cut(s) | 105, 1653, 3210 |
| BsnI | GGCC | 9 cut(s) | 98, 746, 975, 982, 1461, 1513, 2114, 2667, 2834 |
| Bso31I | GGTCTC | 2 cut(s) | 1808, 3238 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1633, 2882 |
| Bsp1407I | TGTACA | 2 cut(s) | 2406, 2692 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1233 |
| Bsp19I | CCATGG | 2 cut(s) | 1610, 2829 |
| BspACI | CCGC | 6 cut(s) | 60, 193, 317, 420, 1601, 1678 |
| BspANI | GGCC | 9 cut(s) | 98, 746, 975, 982, 1461, 1513, 2114, 2667, 2834 |
| BspCNI | CTCAG | 5 cut(s) | 479, 1075, 2628, 2776, 3069 |
| BspDI | ATCGAT | 1 cut(s) | 3105 |
| BspFNI | CGCG | 2 cut(s) | 118, 1603 |
| BspHI | TCATGA | 2 cut(s) | 985, 1330 |
| BspMI | ACCTGC | 1 cut(s) | 274 |
| BspOI | GCTAGC | 1 cut(s) | 2975 |
| BspQI | GCTCTTC | 1 cut(s) | 807 |
| BspT107I | GGYRCC | 3 cut(s) | 29, 70, 996 |
| BspTI | CTTAAG | 1 cut(s) | 910 |
| BspTNI | GGTCTC | 2 cut(s) | 1808, 3238 |
| BsrDI | GCAATG | 4 cut(s) | 62, 492, 937, 1656 |
| BsrFI | RCCGGY | 1 cut(s) | 91 |
| BsrGI | TGTACA | 2 cut(s) | 2406, 2692 |
| BsrI | ACTGG | 4 cut(s) | 756, 1147, 2248, 3248 |
| BssAI | RCCGGY | 1 cut(s) | 91 |
| BssECI | CCNNGG | 9 cut(s) | 734, 1031, 1032, 1514, 1610, 2829, 2843, 2899, 3138 |
| BssNI | GRCGYC | 1 cut(s) | 1177 |
| BssSI | CACGAG | 1 cut(s) | 414 |
| BssT1I | CCWWGG | 3 cut(s) | 1610, 2829, 2843 |
| Bst2BI | CACGAG | 1 cut(s) | 414 |
| Bst6I | CTCTTC | 4 cut(s) | 78, 807, 1201, 1806 |
| BstACI | GRCGYC | 1 cut(s) | 1177 |
| BstAFI | CTTAAG | 1 cut(s) | 910 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 671 |
| BstAUI | TGTACA | 2 cut(s) | 2406, 2692 |
| BstC8I | GCNNGC | 5 cut(s) | 492, 676, 973, 2218, 2973 |
| BstDSI | CCRYGG | 2 cut(s) | 1610, 2829 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 476, 987 |
| BstF5I | GGATG | 6 cut(s) | 432, 760, 1000, 1755, 2068, 2503 |
| BstFNI | CGCG | 2 cut(s) | 118, 1603 |
| BstHHI | GCGC | 1 cut(s) | 1353 |
| BstMAI | GTCTC | 4 cut(s) | 469, 1808, 2772, 3238 |
| BstMCI | CGRYCG | 1 cut(s) | 1363 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 482, 491, 671, 1094, 2180, 2473 |
| BstNSI | RCATGY | 1 cut(s) | 2220 |
| BstUI | CGCG | 2 cut(s) | 118, 1603 |
| BstV1I | GCAGC | 5 cut(s) | 466, 1097, 2167, 2522, 3037 |
| BstV2I | GAAGAC | 1 cut(s) | 2897 |
| BstX2I | RGATCY | 6 cut(s) | 523, 827, 1827, 2080, 2962, 3166 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1557, 1976 |
| BstYI | RGATCY | 6 cut(s) | 523, 827, 1827, 2080, 2962, 3166 |
| Bsu15I | ATCGAT | 1 cut(s) | 3105 |
| Bsu36I | CCTNAGG | 1 cut(s) | 977 |
| BsuI | GTATCC | 1 cut(s) | 2402 |
| BsuRI | GGCC | 9 cut(s) | 98, 746, 975, 982, 1461, 1513, 2114, 2667, 2834 |
| BsuTUI | ATCGAT | 1 cut(s) | 3105 |
| BtgI | CCRYGG | 2 cut(s) | 1610, 2829 |
| BtgZI | GCGATG | 2 cut(s) | 992, 3052 |
| BtsCI | GGATG | 6 cut(s) | 432, 760, 1000, 1755, 2068, 2503 |
| BtsIMutI | CAGTG | 4 cut(s) | 1070, 1624, 2255, 3255 |
| BveI | ACCTGC | 1 cut(s) | 274 |
| Cac8I | GCNNGC | 5 cut(s) | 492, 676, 973, 2218, 2973 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2516 |
| CciI | TCATGA | 2 cut(s) | 985, 1330 |
| CfoI | GCGC | 1 cut(s) | 1353 |
| Cfr10I | RCCGGY | 1 cut(s) | 91 |
| Cfr13I | GGNCC | 6 cut(s) | 593, 1640, 2113, 2204, 2600, 2666 |
| ClaI | ATCGAT | 1 cut(s) | 3105 |
| CseI | GACGC | 1 cut(s) | 107 |
| Csp6I | GTAC | 5 cut(s) | 35, 175, 2211, 2407, 2693 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1046, 1081 |
| CviQI | GTAC | 5 cut(s) | 35, 175, 2211, 2407, 2693 |
| DraI | TTTAAA | 3 cut(s) | 729, 1287, 2136 |
| DraIII | CACNNNGTG | 2 cut(s) | 1486, 2329 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 1647, 2202 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 1647, 2202 |
| EaeI | YGGCCR | 4 cut(s) | 96, 1459, 1511, 2832 |
| Eam1104I | CTCTTC | 4 cut(s) | 78, 807, 1201, 1806 |
| EarI | CTCTTC | 4 cut(s) | 78, 807, 1201, 1806 |
| Eco130I | CCWWGG | 3 cut(s) | 1610, 2829, 2843 |
| Eco147I | AGGCCT | 1 cut(s) | 982 |
| Eco31I | GGTCTC | 2 cut(s) | 1808, 3238 |
| Eco32I | GATATC | 2 cut(s) | 136, 1983 |
| Eco47I | GGWCC | 4 cut(s) | 593, 1640, 2204, 2600 |
| Eco57I | CTGAAG | 2 cut(s) | 221, 2295 |
| Eco81I | CCTNAGG | 1 cut(s) | 977 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 476, 987 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1640, 2204 |
| EcoRV | GATATC | 2 cut(s) | 136, 1983 |
| EcoT14I | CCWWGG | 3 cut(s) | 1610, 2829, 2843 |
| EcoT22I | ATGCAT | 2 cut(s) | 1953, 2535 |
| ErhI | CCWWGG | 3 cut(s) | 1610, 2829, 2843 |
| Esp3I | CGTCTC | 1 cut(s) | 469 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 1165, 1197, 2580, 2612, 2802, 2834 |
| FaqI | GGGAC | 3 cut(s) | 105, 1653, 3210 |
| FauI | CCCGC | 1 cut(s) | 53 |
| FauNDI | CATATG | 1 cut(s) | 2644 |
| FbaI | TGATCA | 1 cut(s) | 2167 |
| Fnu4HI | GCNGC | 6 cut(s) | 420, 455, 1086, 2181, 2511, 3051 |
| FokI | GGATG | 6 cut(s) | 419, 767, 987, 1742, 2075, 2510 |
| Fsp4HI | GCNGC | 6 cut(s) | 420, 455, 1086, 2181, 2511, 3051 |
| GlaI | GCGC | 1 cut(s) | 1352 |
| GluI | GCNGC | 6 cut(s) | 420, 455, 1086, 2181, 2511, 3051 |
| GsaI | CCCAGC | 1 cut(s) | 2560 |
| HaeIII | GGCC | 9 cut(s) | 98, 746, 975, 982, 1461, 1513, 2114, 2667, 2834 |
| HapII | CCGG | 4 cut(s) | 52, 92, 129, 2835 |
| HgaI | GACGC | 1 cut(s) | 107 |
| HhaI | GCGC | 1 cut(s) | 1353 |
| Hin1I | GRCGYC | 1 cut(s) | 1177 |
| Hin6I | GCGC | 1 cut(s) | 1351 |
| HinP1I | GCGC | 1 cut(s) | 1351 |
| HincII | GTYRAC | 1 cut(s) | 1966 |
| HindII | GTYRAC | 1 cut(s) | 1966 |
| HindIII | AAGCTT | 3 cut(s) | 907, 1316, 2025 |
| HpaII | CCGG | 4 cut(s) | 52, 92, 129, 2835 |
| HphI | GGTGA | 3 cut(s) | 634, 1091, 1502 |
| Hpy166II | GTNNAC | 7 cut(s) | 37, 1624, 1966, 2195, 2651, 2695, 2765 |
| Hpy8I | GTNNAC | 7 cut(s) | 37, 1624, 1966, 2195, 2651, 2695, 2765 |
| Hpy99I | CGWCG | 1 cut(s) | 10 |
| HpyAV | CCTTC | 6 cut(s) | 586, 602, 983, 1512, 1513, 2842 |
| HpyCH4IV | ACGT | 3 cut(s) | 247, 874, 1177 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 482, 491, 671, 1094, 2180, 2473 |
| HpySE526I | ACGT | 3 cut(s) | 247, 874, 1177 |
| Hsp92I | GRCGYC | 1 cut(s) | 1177 |
| HspAI | GCGC | 1 cut(s) | 1351 |
| Ksp22I | TGATCA | 1 cut(s) | 2167 |
| LguI | GCTCTTC | 1 cut(s) | 807 |
| LmnI | GCTCC | 6 cut(s) | 303, 1030, 1585, 1711, 2885, 3124 |
| Lsp1109I | GCAGC | 5 cut(s) | 466, 1097, 2167, 2522, 3037 |
| LweI | GCATC | 7 cut(s) | 218, 637, 674, 958, 1201, 1938, 1960 |
| MaeII | ACGT | 3 cut(s) | 247, 874, 1177 |
| MaeIII | GTNAC | 7 cut(s) | 195, 952, 1389, 1490, 1652, 1937, 3092 |
| MfeI | CAATTG | 2 cut(s) | 162, 703 |
| MflI | RGATCY | 6 cut(s) | 523, 827, 1827, 2080, 2962, 3166 |
| MhlI | GDGCHC | 2 cut(s) | 1633, 2882 |
| MlsI | TGGCCA | 2 cut(s) | 98, 1513 |
| MluNI | TGGCCA | 2 cut(s) | 98, 1513 |
| MlyI | GAGTC | 4 cut(s) | 38, 1063, 2774, 2942 |
| MmeI | TCCRAC | 1 cut(s) | 66 |
| Mox20I | TGGCCA | 2 cut(s) | 98, 1513 |
| Mph1103I | ATGCAT | 2 cut(s) | 1953, 2535 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1596, 2860 |
| MscI | TGGCCA | 2 cut(s) | 98, 1513 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1335, 2221 |
| Msp20I | TGGCCA | 2 cut(s) | 98, 1513 |
| MspA1I | CMGCKG | 1 cut(s) | 1678 |
| MspCI | CTTAAG | 1 cut(s) | 910 |
| MspI | CCGG | 4 cut(s) | 52, 92, 129, 2835 |
| MunI | CAATTG | 2 cut(s) | 162, 703 |
| MvnI | CGCG | 2 cut(s) | 118, 1603 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 482, 491, 671, 1094, 2180, 2473 |
| NcoI | CCATGG | 2 cut(s) | 1610, 2829 |
| NdeI | CATATG | 1 cut(s) | 2644 |
| NheI | GCTAGC | 1 cut(s) | 2971 |
| NmuCI | GTSAC | 5 cut(s) | 1389, 1490, 1652, 1937, 3092 |
| NsiI | ATGCAT | 2 cut(s) | 1953, 2535 |
| NspI | RCATGY | 1 cut(s) | 2220 |
| PaeI | GCATGC | 1 cut(s) | 2220 |
| PagI | TCATGA | 2 cut(s) | 985, 1330 |
| PasI | CCCWGGG | 1 cut(s) | 1032 |
| PceI | AGGCCT | 1 cut(s) | 982 |
| PciSI | GCTCTTC | 1 cut(s) | 807 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1596, 2860 |
| PfeI | GAWTC | 6 cut(s) | 1541, 2341, 2822, 3032, 3062, 3134 |
| PflMI | CCANNNNNTGG | 3 cut(s) | 359, 511, 2556 |
| PfoI | TCCNGGA | 2 cut(s) | 2006, 2394 |
| PkrI | GCNGC | 6 cut(s) | 421, 456, 1087, 2182, 2512, 3052 |
| PleI | GAGTC | 4 cut(s) | 38, 1062, 2774, 2942 |
| PpsI | GAGTC | 4 cut(s) | 38, 1062, 2774, 2942 |
| PpuMI | RGGWCCY | 2 cut(s) | 1640, 2204 |
| PshBI | ATTAAT | 2 cut(s) | 963, 2751 |
| PsiI | TTATAA | 1 cut(s) | 1530 |
| Psp5II | RGGWCCY | 2 cut(s) | 1640, 2204 |
| PspFI | CCCAGC | 1 cut(s) | 2556 |
| PspPI | GGNCC | 6 cut(s) | 593, 1640, 2113, 2204, 2600, 2666 |
| PspPPI | RGGWCCY | 2 cut(s) | 1640, 2204 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 167, 199 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2516 |
| PsuI | RGATCY | 6 cut(s) | 523, 827, 1827, 2080, 2962, 3166 |
| RsaI | GTAC | 5 cut(s) | 36, 176, 2212, 2408, 2694 |
| RsaNI | GTAC | 5 cut(s) | 35, 175, 2211, 2407, 2693 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1335, 2221 |
| SapI | GCTCTTC | 1 cut(s) | 807 |
| SatI | GCNGC | 6 cut(s) | 420, 455, 1086, 2181, 2511, 3051 |
| Sau96I | GGNCC | 6 cut(s) | 593, 1640, 2113, 2204, 2600, 2666 |
| SchI | GAGTC | 4 cut(s) | 38, 1063, 2774, 2942 |
| SduI | GDGCHC | 2 cut(s) | 1633, 2882 |
| SfaNI | GCATC | 7 cut(s) | 218, 637, 674, 958, 1201, 1938, 1960 |
| SinI | GGWCC | 4 cut(s) | 593, 1640, 2204, 2600 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1335, 2221 |
| SmlI | CTYRAG | 4 cut(s) | 910, 1890, 3043, 3282 |
| SmoI | CTYRAG | 4 cut(s) | 910, 1890, 3043, 3282 |
| SphI | GCATGC | 1 cut(s) | 2220 |
| SseBI | AGGCCT | 1 cut(s) | 982 |
| SsiI | CCGC | 6 cut(s) | 60, 193, 317, 420, 1601, 1678 |
| SspI | AATATT | 2 cut(s) | 1327, 2749 |
| StuI | AGGCCT | 1 cut(s) | 982 |
| StyI | CCWWGG | 3 cut(s) | 1610, 2829, 2843 |
| TaiI | ACGT | 3 cut(s) | 250, 877, 1180 |
| TaqI | TCGA | 4 cut(s) | 538, 2310, 2545, 3105 |
| TaqII | GACCGA | 1 cut(s) | 3080 |
| TatI | WGTACW | 3 cut(s) | 174, 2406, 2692 |
| TauI | GCSGC | 1 cut(s) | 422 |
| TfiI | GAWTC | 6 cut(s) | 1541, 2341, 2822, 3032, 3062, 3134 |
| TscAI | CASTG | 4 cut(s) | 1070, 1631, 2255, 3255 |
| TseFI | GTSAC | 5 cut(s) | 1389, 1490, 1652, 1937, 3092 |
| TseI | GCWGC | 5 cut(s) | 454, 1085, 2180, 2510, 3050 |
| Tsp45I | GTSAC | 5 cut(s) | 1389, 1490, 1652, 1937, 3092 |
| TspGWI | ACGGA | 2 cut(s) | 425, 451 |
| TspRI | CASTG | 4 cut(s) | 1070, 1631, 2255, 3255 |
| Van91I | CCANNNNNTGG | 3 cut(s) | 359, 511, 2556 |
| Vha464I | CTTAAG | 1 cut(s) | 910 |
| VpaK11BI | GGWCC | 4 cut(s) | 593, 1640, 2204, 2600 |
| VspI | ATTAAT | 2 cut(s) | 963, 2751 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 476, 987 |
| XapI | RAATTY | 7 cut(s) | 551, 638, 1377, 1552, 2813, 2989, 3266 |
| XbaI | TCTAGA | 1 cut(s) | 778 |
| XceI | RCATGY | 1 cut(s) | 2220 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2255 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1596, 2860 |
| ZraI | GACGTC | 1 cut(s) | 1178 |
| Zsp2I | ATGCAT | 2 cut(s) | 1953, 2535 |