Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001577

Physical Location & Seq

Genomic Coordinates Reverse (-)

28909004 .. 28914900

Gene Structure 5897 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1210300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3396 bp

ATGTCGTCGCAGAAGCTCATTGCCAACGGTTCTGTTAAGTCCGATGCAGTGAATATGAACGTGGGGCCTTTCTCGTCCATGCCGATTTTCGACGAGCTGCCGGTCGCCACCATTGTATCAGTTTCGCGGCCCGATACCGGAGATATTAGCCCCATGCTTCTGTCTTACACCATTGAGTTTCAGAGAAAACGTGAGTGTGAAAATCACTACGTGTATCCTACTGGTAGTTTTTGGTTCAAGTGGCGGTTGCTGAAGAAAGCGTCACAAGTTCTCTACTTGCATTTTGCTTTGAAGAAACGTGCGATAATTGAGGAGTTTCATGAGAAGCAAGAGCAGGTTAAAGAATGGCTACATAGCATAGGAATAGTTGACCAGACAGCGGTAGTACAAGACGATGATGAACCAGATGATGGGGCTGTTCCTTTACATCACGACGAAAGTGTTAAAAACAGATATGTTCCATCTCGTGCTGCTTTGCCTATCCTCCGTCCAGCATTAGGACGCCAGCAGTCCATTTCAGACAGAGCAAAGGTGGCAATGCAGGGTTATTTGAACCATTTTATGGGAAACATGAATATAGTAAATTCTCGAGAGGTCTGCAAATTCTTGGAGGTCTCGAAGCTCTCCTTTTTACAGGAGTATGGACCAAAGTTGAAAGAAGGTTATGTAATGGTGAAGCATCTACCAAAATGTACCGGGGCTAATTCTGATGTTAAATCTTGCGCGTACTTTTTTCTTGATTGTTGCAGCAACAACTGGCAAAAGGTGTGGGCAGTTTTAAAGCCCGGGTTCCTGGCCTTATTGGAGGATCCTTTAGATACCAACCTTTTAGACATTATTGTTTTCAATGTATTGCCAGCTTCCAATGGAGATGGATGGTCTCAAATATATTTGGCTAATCAAATAAAGGAGCATAATCCTCTACGTTATACATTTAGGGTTGCTTGTGGAAATCACAGCTTAAGGTTAAGAGCCACTACCAATGCTAAAGTTAAAGAATGGGTTACCGCGATCAATGATGCTGTTTCAAGGCCTCATGAGGGTTGGTGTCATCCTCATCGGTTTGGTTCCTTTGCTCCTCCAAGGGGTTTGACTGATGACGGAAGTCAAGCCCAATGGTTTGTAGATGGCGAGGCAGCATTTGAAGCTATTGCTTCATCTATCGAAGGTGCAAAATCAGAGATATTCATTACTGGCTGGTGGCTTTGCCCTGAACTGTATCTAAGACGTCCTTTTGATAGCAATTCGTCTTCCCGGCTTGATGCATTACTAGAAGAAAAGGCTAAGCAAGGTGTTCAGATTTACATTCTTCTCTACAAGGAGGTTTCTATTGCTTTAAAGATCAACAGTTCATACAGCAAGAAATTGCTTTCGAACATTCATGAGAATGTGAGAGTATTGCGCCATCCTAACCGTTTTCCCACAGGAATTTATTTATGGTCACATCATGAAAAAATTGTCATTGTTGATTACCAGATTTGCTACATTGGAGGATTAGATTTGTGCTTTGGCCGCTATGACACGGTTGAACATAAAGTGGGCGACTGCCCTCCTCATATATGGCCTGGAAAGGACTATTATAACCCAAGAGAATCCGAACCAAATTCTTGGGAAGACGCACTTAAAGATGAATTAGAGCGTGAAAAGTATCCGCGTATGCCTTGGCATGATGTCCACTGTGCTCTTTGGGGACCGCCCTGTCGTGACATTGCAAGGCACTTTGTTCAGCGCTGGAACCATGCTAAGAGAAACAAAGCTCCAAATGAACAGACAATTCCGCTACTTATGCCTCACCACCACATGGTCCTTCCGCATTACATGGGAAGAAGTAGAGAGATGGACATTAAGAAGAAAAACAAAGAAGACAACCAGAATGGGACCAACAGAGAGAATTTTTCCTCACCATCACCGGTGCAAGATATTCCATTGCTTTGGCCTCAAGAAGCGGATGGTTTAGATGCTCCTATTTTAGACCAAAAGTTACGCGCCATGCGCTTGAATCAGAATCTTCTCAACCAGCCAACTGATGGTTTCATAGATGACCTTCATTCTAGGGATCTTAAAAGTGAAGCAAATTTAAACACGGTGACACAGTCTGGCTCAACAACTCCAAATGAATGGTCAGAAAGTTCAGACAATGGCGATCATGCTGTTGCTGCTGATGATTATGGACAAATAGGTCCTCGTACTGCATGCGAAATTCAGGTTGTAAGAAGTGTGAGCCAGTGGTCTGCTGGGTCTAGTCAAACGGAAGAAAGCATACATAGTGCTTATTGTTCTCTCATTGAGAAGGCAGAACATTTTGTGTATATTGAGAATCAATTTTTTATATCAGGTCTTTCAGGGGATGACATTATACAGAATCGTGTCCTAGAATCGTTGTACAGTCGTATTGTGCTAGCACACAAAGAACAAAAGTGTTTCAGAGTTATTGTTGTCATACCACTATTACCCGGCTTTCAGGGTGGTGTGGACGATGGTGGTGCAGCTACTGTTAGAGCTATAATGCATTGGCAGTACCGGACGATTAGCTGGGGAAAACACTCAATACTGCAAAAGCTTAAAGTTAAGCTTGGTACCAAGACACATGATTACATTTCTTTCTACGGTCTGAGGACATATGGCAGACTTTTCGAAGGTGGCCCTGTGGCCACAAGTCAGGTTTATGTGCACAGCAAAGTGATGATCATAGATGATTGCATTTCCTTCATCGGATCATCTAATATTAATGACAGAAGTCTGCTTGGATCAAGAGACTCTGAGATTGGTGTGGTAATTGAGGATAAAGAGTTTTTCGAATCATCTATGAATGGACAGCCTTGGAAGGCCGGAAAATTTGCGCATAGTCTTAGGTGCTCCTTGTGGTCCGAGCACCTGGGTCTTCTTGCAGGGGAGATACATCAAATCAATGATCCTGTATCAGACTCAACATACAAAGATCTGTGGTTAGCAACTGCAAAGGAAAACTCAACTGTTTACCAAGATGTCTTTTCTTGCATCCCAAGTGATTCTATCCACTCAAGGTACAGTACTACCAAGGTTTATCGTCCCGCAAACAAAATTTATGTACCAACGTTTTGTATTTTCGTTTCTTTGATGTATGAACTCTTAAATCTCTACTTTTTTTTCCCATCAAACTACAGAACTGCTCTCAGGCAATGCAGGGCTCAATGGAAAGAGAAACATGGCCACACGACGATCGATTTGGGAATAGCCCCCGAGAAGCTACTACAGTCTTGTGAAGGTGGAGAGGTGAAAGAAATAGATCCGATGGAAAGGCTGAAGCATGTAAGGGGACATGTTGTTTCCTTCCCTTTAGAGTTTATGCAGCAAGAAGATTTGAGACCAGTCTTCAATGAAAGTCAATTTTACACTTCCCCTCAAGTATTTCGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004620 GO:0004630 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0006629 GO:0006643 GO:0006644 GO:0006664 GO:0006793 GO:0006796 GO:0006950 GO:0006995 GO:0007154 GO:0007275 GO:0008081 GO:0008150 GO:0008152 GO:0008610 GO:0009056 GO:0009058 GO:0009247 GO:0009267 GO:0009395 GO:0009605 GO:0009719 GO:0009725 GO:0009733 GO:0009987 GO:0009991 GO:0010033 GO:0016036 GO:0016042 GO:0016298 GO:0016787 GO:0016788 GO:0019374 GO:0019375 GO:0019637 GO:0022622 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0032879 GO:0033554 GO:0042221 GO:0042578 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043562 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0046434 GO:0046467 GO:0048364 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051716 GO:0060627 GO:0065007 GO:0071496 GO:0071704 GO:0099402 GO:1901135 GO:1901137 GO:1901575 GO:1901576 GO:1903509

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

PH PLDc PLDc_2

Protein Analysis

1132

Amino Acids

128.94

Weight (kDa)

6.49

Isoelectric Point (pI)

43.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PLDc	PF00614	478 - 505	2e-07	Phospholipase D Active site motif
PLDc_2	PF13091	884 - 933	1.8e-08	PLD-like domain
PLDc	PF00614	890 - 913	3.6e-06	Phospholipase D Active site motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001577)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16785 AT3G16785 AT3G16785 AT3G16785 AT3G16785 AT3G16785
fragaria_vesca	FvH4_4g18810 FvH4_6g06940 FvH4_6g06940 FvH4_6g06940
malus_domestica	MD04G1197500.v1.1 MD12G1210300.v1.1 MD13G1162600.v1.1 MD16G1162400.v1.1
prunus_persica	Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.6G317900_v2.0.a1 Prupe.6G317900_v2.0.a1 Prupe.6G317900_v2.0.a1
pyrus_communis	pycom04g17480 pycom13g13890 pycom16g13770
rosa_chinensis	RchiOBHm_Chr3g0456481 RchiOBHm_Chr4g0423621
rosa_laevigata	RLG00000007541 RLG00000025302
rosa_multiflora	Rmu_co8413375.1_g000001 Rmu_sc0000464.1_g000017 Rmu_sc0002607.1_g000011 Rmu_sc0008373.1_g000012 Rmu_sc0016309.1_g000001
rosa_roxburghii	Rroxscaffold_5G00365830 Rroxscaffold_6G00422720
rosa_rugosa	Rorug03G0013400 Rorug03G0013400 Rorug03G0013400 Rorug04G0189100
rosa_samantha	Rh3AG073700 Rh3BG075600 Rh3DG076900 Rh4AG248700 Rh4BG254000 Rh4DG249000
rosa_wichuraiana	Rw3G006320 Rw4G021300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1581
AasI	GACNNNNNNGTC	2 cut(s)	1698, 2178
AatII	GACGTC	1 cut(s)	1231
Acc16I	TGCGCA	1 cut(s)	2841
Acc36I	ACCTGC	1 cut(s)	325
Acc65I	GGTACC	1 cut(s)	2576
AccB1I	GGYRCC	1 cut(s)	2576
AccB7I	CCANNNNNTGG	4 cut(s)	410, 562, 1801, 2027
AccII	CGCG	5 cut(s)	127, 725, 1010, 1654, 1986
AclI	AACGTT	1 cut(s)	3074
AclWI	GGATC	7 cut(s)	803, 816, 2064, 2722, 2755, 2906, 3260
AcoI	YGGCCR	3 cut(s)	1510, 2649, 3187
AcsI	RAATTY	9 cut(s)	583, 602, 1428, 1603, 1891, 2074, 2199, 2834, 3060
AcuI	CTGAAG	2 cut(s)	272, 3302
AcyI	GRCGYC	2 cut(s)	502, 1228
AdeI	CACNNNGTG	1 cut(s)	211
AfeI	AGCGCT	1 cut(s)	1730
AfiI	CCNNNNNNNGG	9 cut(s)	137, 379, 410, 497, 562, 1040, 1085, 1801, 2027
AflII	CTTAAG	1 cut(s)	961
AflIII	ACRYGT	2 cut(s)	210, 3298
AgeI	ACCGGT	1 cut(s)	1909
AjnI	CCWGG	3 cut(s)	792, 1564, 2874
Alw21I	GWGCWC	4 cut(s)	1684, 2673, 2858, 2874
Alw26I	GTCTC	4 cut(s)	619, 885, 2748, 3337
Alw44I	GTGCAC	1 cut(s)	2669
AlwI	GGATC	7 cut(s)	803, 816, 2064, 2722, 2755, 2906, 3260
AlwNI	CAGNNNCTG	1 cut(s)	2492
Ama87I	CYCGRG	3 cut(s)	588, 785, 3218
Aor51HI	AGCGCT	1 cut(s)	1730
ApaLI	GTGCAC	1 cut(s)	2669
ApeKI	GCWGC	7 cut(s)	97, 470, 747, 1136, 2156, 2486, 3328
ApoI	RAATTY	9 cut(s)	583, 602, 1428, 1603, 1891, 2074, 2199, 2834, 3060
ArsI	GACNNNNNNTTYG	4 cut(s)	2105, 2137, 3187, 3219
AseI	ATTAAT	1 cut(s)	2727
AsiGI	ACCGGT	1 cut(s)	1909
Asp718I	GGTACC	1 cut(s)	2576
AspLEI	GCGC	6 cut(s)	725, 1404, 1731, 1988, 1995, 2842
AspS9I	GGNCC	9 cut(s)	65, 129, 644, 1691, 1804, 1878, 2180, 2642, 2865
AsuC2I	CCSGG	5 cut(s)	697, 786, 787, 1255, 2454
AsuHPI	GGTGA	6 cut(s)	685, 1784, 1893, 1899, 2098, 3265
AsuII	TTCGAA	3 cut(s)	1373, 2634, 2796
AsuNHI	GCTAGC	1 cut(s)	2398
AvaI	CYCGRG	3 cut(s)	588, 785, 3218
AvaII	GGWCC	6 cut(s)	644, 1691, 1804, 1878, 2180, 2865
BaeGI	GKGCMC	1 cut(s)	2673
BaeI	ACNNNNGTAYC	2 cut(s)	99, 132
BalI	TGGCCA	2 cut(s)	2651, 3189
BamHI	GGATCC	1 cut(s)	808
BanI	GGYRCC	1 cut(s)	2576
BanII	GRGCYC	1 cut(s)	3169
BarI	GAAGNNNNNNTAC	2 cut(s)	2244, 2276
BauI	CACGAG	1 cut(s)	465
BbsI	GAAGAC	5 cut(s)	1242, 1620, 1869, 2873, 3343
Bbv12I	GWGCWC	4 cut(s)	1684, 2673, 2858, 2874
BbvI	GCAGC	7 cut(s)	84, 457, 759, 1148, 2143, 2498, 3340
BcgI	CGANNNNNNTGC	6 cut(s)	579, 613, 2465, 2499, 2614, 2648
BciT130I	CCWGG	3 cut(s)	794, 1566, 2876
BciVI	GTATCC	2 cut(s)	225, 1659
BclI	TGATCA	1 cut(s)	2685
BcnI	CCSGG	5 cut(s)	697, 786, 787, 1255, 2454
BcoDI	GTCTC	4 cut(s)	619, 885, 2748, 3337
BfaI	CTAG	5 cut(s)	1271, 2052, 2241, 2372, 2399
BfmI	CTRYAG	2 cut(s)	3139, 3230
BfoI	RGCGCY	1 cut(s)	1732
BfrI	CTTAAG	1 cut(s)	961
BfuAI	ACCTGC	1 cut(s)	325
BfuI	GTATCC	2 cut(s)	225, 1659
BglII	AGATCT	1 cut(s)	2938
BisI	GCNGC	9 cut(s)	98, 128, 471, 748, 1137, 1513, 2157, 2487, 3329
BlpI	GCTNAGC	1 cut(s)	1284
BlsI	GCNGC	9 cut(s)	99, 129, 472, 749, 1138, 1514, 2158, 2488, 3330
BmcAI	AGTACT	1 cut(s)	3031
Bme1390I	CCNGG	8 cut(s)	697, 786, 787, 794, 1255, 1566, 2454, 2876
Bme18I	GGWCC	6 cut(s)	644, 1691, 1804, 1878, 2180, 2865
BmeT110I	CYCGRG	3 cut(s)	588, 785, 3218
BmgT120I	GGNCC	9 cut(s)	65, 129, 644, 1691, 1804, 1878, 2180, 2642, 2865
BmiI	GGNNCC	8 cut(s)	66, 791, 810, 1069, 1692, 1736, 1879, 2578
BmrFI	CCNGG	8 cut(s)	697, 786, 787, 794, 1255, 1566, 2454, 2876
BmsI	GCATC	6 cut(s)	34, 688, 1009, 1252, 1948, 3006
BmtI	GCTAGC	1 cut(s)	2402
BoxI	GACNNNNGTC	2 cut(s)	1104, 2736
BpiI	GAAGAC	5 cut(s)	1242, 1620, 1869, 2873, 3343
Bpu1102I	GCTNAGC	1 cut(s)	1284
Bpu14I	TTCGAA	3 cut(s)	1373, 2634, 2796
BpuEI	CTTGAG	3 cut(s)	1923, 3004, 3366
BpuMI	CCSGG	5 cut(s)	697, 786, 787, 1255, 2454
Bsa29I	ATCGAT	1 cut(s)	3201
BsaAI	YACGTR	1 cut(s)	211
BsaHI	GRCGYC	2 cut(s)	502, 1228
BsaI	GGTCTC	3 cut(s)	619, 885, 3337
BsaJI	CCNNGG	7 cut(s)	696, 785, 1082, 1661, 2819, 2875, 3036
BsaWI	WCCGGW	3 cut(s)	137, 1909, 2520
Bsc4I	CCNNNNNNNGG	9 cut(s)	137, 379, 410, 497, 562, 1040, 1085, 1801, 2027
Bse118I	RCCGGY	2 cut(s)	100, 1909
Bse1I	ACTGG	5 cut(s)	226, 761, 1198, 2224, 3347
Bse3DI	GCAATG	5 cut(s)	18, 543, 1707, 1925, 3164
BseBI	CCWGG	3 cut(s)	794, 1566, 2876
BseCI	ATCGAT	1 cut(s)	3201
BseDI	CCNNGG	7 cut(s)	696, 785, 1082, 1661, 2819, 2875, 3036
BseGI	GGATG	6 cut(s)	881, 1051, 1405, 1954, 2353, 2997
BseLI	CCNNNNNNNGG	9 cut(s)	137, 379, 410, 497, 562, 1040, 1085, 1801, 2027
BseMI	GCAATG	5 cut(s)	18, 543, 1707, 1925, 3164
BseMII	CTCAG	3 cut(s)	2603, 2751, 3166
BseNI	ACTGG	5 cut(s)	226, 761, 1198, 2224, 3347
BseRI	GAGGAG	3 cut(s)	326, 1068, 1542
BseSI	GKGCMC	1 cut(s)	2673
BseXI	GCAGC	7 cut(s)	84, 457, 759, 1148, 2143, 2498, 3340
BseYI	CCCAGC	2 cut(s)	2234, 2532
BsgI	GTGCAG	1 cut(s)	2505
Bsh1236I	CGCG	5 cut(s)	127, 725, 1010, 1654, 1986
Bsh1285I	CGRYCG	2 cut(s)	105, 3201
BshNI	GGYRCC	1 cut(s)	2576
BshTI	ACCGGT	1 cut(s)	1909
BshVI	ATCGAT	1 cut(s)	3201
BsiEI	CGRYCG	2 cut(s)	105, 3201
BsiHKAI	GWGCWC	4 cut(s)	1684, 2673, 2858, 2874
BsiHKCI	CYCGRG	3 cut(s)	588, 785, 3218
BsiSI	CCGG	9 cut(s)	101, 138, 696, 786, 1255, 1910, 2454, 2521, 2829
BslFI	GGGAC	4 cut(s)	1704, 1891, 3033, 3309
BslI	CCNNNNNNNGG	9 cut(s)	137, 379, 410, 497, 562, 1040, 1085, 1801, 2027
BsmAI	GTCTC	4 cut(s)	619, 885, 2748, 3337
BsmFI	GGGAC	4 cut(s)	1704, 1891, 3033, 3309
Bso31I	GGTCTC	3 cut(s)	619, 885, 3337
BsoBI	CYCGRG	3 cut(s)	588, 785, 3218
Bsp119I	TTCGAA	3 cut(s)	1373, 2634, 2796
Bsp1286I	GDGCHC	5 cut(s)	1684, 2673, 2858, 2874, 3169
Bsp1407I	TGTACA	1 cut(s)	2382
Bsp1720I	GCTNAGC	1 cut(s)	1284
BspCNI	CTCAG	3 cut(s)	2604, 2752, 3165
BspDI	ATCGAT	1 cut(s)	3201
BspFNI	CGCG	5 cut(s)	127, 725, 1010, 1654, 1986
BspHI	TCATGA	4 cut(s)	319, 1036, 1381, 1447
BspLI	GGNNCC	8 cut(s)	66, 791, 810, 1069, 1692, 1736, 1879, 2578
BspMI	ACCTGC	1 cut(s)	325
BspOI	GCTAGC	1 cut(s)	2402
BspPI	GGATC	7 cut(s)	803, 816, 2064, 2722, 2755, 2906, 3260
BspT104I	TTCGAA	3 cut(s)	1373, 2634, 2796
BspT107I	GGYRCC	1 cut(s)	2576
BspTI	CTTAAG	1 cut(s)	961
BspTNI	GGTCTC	3 cut(s)	619, 885, 3337
BsrDI	GCAATG	5 cut(s)	18, 543, 1707, 1925, 3164
BsrFI	RCCGGY	2 cut(s)	100, 1909
BsrGI	TGTACA	1 cut(s)	2382
BsrI	ACTGG	5 cut(s)	226, 761, 1198, 2224, 3347
BssAI	RCCGGY	2 cut(s)	100, 1909
BssECI	CCNNGG	7 cut(s)	696, 785, 1082, 1661, 2819, 2875, 3036
BssNI	GRCGYC	2 cut(s)	502, 1228
BssSI	CACGAG	1 cut(s)	465
BssT1I	CCWWGG	4 cut(s)	1082, 1661, 2819, 3036
Bst2BI	CACGAG	1 cut(s)	465
Bst2UI	CCWGG	3 cut(s)	794, 1566, 2876
BstACI	GRCGYC	2 cut(s)	502, 1228
BstAFI	CTTAAG	1 cut(s)	961
BstAUI	TGTACA	1 cut(s)	2382
BstBAI	YACGTR	1 cut(s)	211
BstBI	TTCGAA	3 cut(s)	1373, 2634, 2796
BstC8I	GCNNGC	4 cut(s)	506, 858, 2194, 2400
BstDEI	CTNAG	7 cut(s)	1223, 1284, 1743, 2612, 2760, 2849, 3152
BstEII	GGTNACC	1 cut(s)	1003
BstF5I	GGATG	6 cut(s)	881, 1051, 1405, 1954, 2353, 2997
BstFNI	CGCG	5 cut(s)	127, 725, 1010, 1654, 1986
BstH2I	RGCGCY	1 cut(s)	1732
BstHHI	GCGC	6 cut(s)	725, 1404, 1731, 1988, 1995, 2842
BstMAI	GTCTC	4 cut(s)	619, 885, 2748, 3337
BstMCI	CGRYCG	2 cut(s)	105, 3201
BstMWI	GCNNNNNNNGC	6 cut(s)	533, 1145, 1512, 1786, 1992, 2156
BstNI	CCWGG	3 cut(s)	794, 1566, 2876
BstNSI	RCATGY	3 cut(s)	2196, 3290, 3302
BstPAI	GACNNNNGTC	2 cut(s)	1104, 2736
BstPI	GGTNACC	1 cut(s)	1003
BstSCI	CCNGG	8 cut(s)	695, 784, 785, 792, 1253, 1564, 2452, 2874
BstSFI	CTRYAG	2 cut(s)	3139, 3230
BstSLI	GKGCMC	1 cut(s)	2673
BstUI	CGCG	5 cut(s)	127, 725, 1010, 1654, 1986
BstV1I	GCAGC	7 cut(s)	84, 457, 759, 1148, 2143, 2498, 3340
BstV2I	GAAGAC	5 cut(s)	1242, 1620, 1869, 2873, 3343
BstX2I	RGATCY	4 cut(s)	808, 2056, 2938, 3265
BstXI	CCANNNNNNTGG	3 cut(s)	1608, 1932, 2118
BstYI	RGATCY	4 cut(s)	808, 2056, 2938, 3265
Bsu15I	ATCGAT	1 cut(s)	3201
BsuI	GTATCC	2 cut(s)	225, 1659
BsuTUI	ATCGAT	1 cut(s)	3201
BtsCI	GGATG	6 cut(s)	881, 1051, 1405, 1954, 2353, 2997
BtsI	GCAGTG	1 cut(s)	54
BtsIMutI	CAGTG	3 cut(s)	54, 1675, 2231
BveI	ACCTGC	1 cut(s)	325
Cac8I	GCNNGC	4 cut(s)	506, 858, 2194, 2400
CaiI	CAGNNNCTG	1 cut(s)	2492
CciI	TCATGA	4 cut(s)	319, 1036, 1381, 1447
CfoI	GCGC	6 cut(s)	725, 1404, 1731, 1988, 1995, 2842
Cfr10I	RCCGGY	2 cut(s)	100, 1909
Cfr13I	GGNCC	9 cut(s)	65, 129, 644, 1691, 1804, 1878, 2180, 2642, 2865
Cfr9I	CCCGGG	1 cut(s)	785
ClaI	ATCGAT	1 cut(s)	3201
CseI	GACGC	3 cut(s)	249, 510, 1625
CspAI	ACCGGT	1 cut(s)	1909
CspCI	CAANNNNNGTGG	2 cut(s)	749, 784
DdeI	CTNAG	7 cut(s)	1223, 1284, 1743, 2612, 2760, 2849, 3152
DraI	TTTAAA	3 cut(s)	780, 1338, 2079
DraIII	CACNNNGTG	1 cut(s)	211
DrdI	GACNNNNNNGTC	2 cut(s)	1698, 2178
DseDI	GACNNNNNNGTC	2 cut(s)	1698, 2178
EaeI	YGGCCR	3 cut(s)	1510, 2649, 3187
Eco130I	CCWWGG	4 cut(s)	1082, 1661, 2819, 3036
Eco147I	AGGCCT	1 cut(s)	1033
Eco24I	GRGCYC	1 cut(s)	3169
Eco31I	GGTCTC	3 cut(s)	619, 885, 3337
Eco47I	GGWCC	6 cut(s)	644, 1691, 1804, 1878, 2180, 2865
Eco47III	AGCGCT	1 cut(s)	1730
Eco57I	CTGAAG	2 cut(s)	272, 3302
Eco88I	CYCGRG	3 cut(s)	588, 785, 3218
Eco91I	GGTNACC	1 cut(s)	1003
EcoO109I	RGGNCCY	2 cut(s)	65, 2180
EcoO65I	GGTNACC	1 cut(s)	1003
EcoRII	CCWGG	3 cut(s)	792, 1564, 2874
EcoT14I	CCWWGG	4 cut(s)	1082, 1661, 2819, 3036
EcoT22I	ATGCAT	2 cut(s)	1267, 2511
EcoT38I	GRGCYC	1 cut(s)	3169
ErhI	CCWWGG	4 cut(s)	1082, 1661, 2819, 3036
FalI	AAGNNNNNCTT	8 cut(s)	611, 643, 1216, 1248, 1605, 1637, 2556, 2588
FaqI	GGGAC	4 cut(s)	1704, 1891, 3033, 3309
FauI	CCCGC	1 cut(s)	3058
FauNDI	CATATG	1 cut(s)	2620
FbaI	TGATCA	1 cut(s)	2685
Fnu4HI	GCNGC	9 cut(s)	98, 128, 471, 748, 1137, 1513, 2157, 2487, 3329
FokI	GGATG	6 cut(s)	888, 1038, 1392, 1961, 2360, 2984
FriOI	GRGCYC	1 cut(s)	3169
Fsp4HI	GCNGC	9 cut(s)	98, 128, 471, 748, 1137, 1513, 2157, 2487, 3329
FspBI	CTAG	5 cut(s)	1271, 2052, 2241, 2372, 2399
FspI	TGCGCA	1 cut(s)	2841
GlaI	GCGC	6 cut(s)	724, 1403, 1730, 1987, 1994, 2841
GluI	GCNGC	9 cut(s)	98, 128, 471, 748, 1137, 1513, 2157, 2487, 3329
GsaI	CCCAGC	2 cut(s)	2238, 2536
HaeII	RGCGCY	1 cut(s)	1732
HapII	CCGG	9 cut(s)	101, 138, 696, 786, 1255, 1910, 2454, 2521, 2829
HgaI	GACGC	3 cut(s)	249, 510, 1625
HhaI	GCGC	6 cut(s)	725, 1404, 1731, 1988, 1995, 2842
Hin1I	GRCGYC	2 cut(s)	502, 1228
Hin6I	GCGC	6 cut(s)	723, 1402, 1729, 1986, 1993, 2840
HinP1I	GCGC	6 cut(s)	723, 1402, 1729, 1986, 1993, 2840
HincII	GTYRAC	1 cut(s)	370
HindII	GTYRAC	1 cut(s)	370
HindIII	AAGCTT	2 cut(s)	2558, 2570
HpaII	CCGG	9 cut(s)	101, 138, 696, 786, 1255, 1910, 2454, 2521, 2829
HphI	GGTGA	6 cut(s)	685, 1784, 1893, 1899, 2098, 3265
Hpy166II	GTNNAC	5 cut(s)	370, 1675, 2473, 2671, 2977
Hpy8I	GTNNAC	5 cut(s)	370, 1675, 2473, 2671, 2977
Hpy99I	CGWCG	4 cut(s)	10, 95, 437, 3199
HpyCH4IV	ACGT	7 cut(s)	60, 190, 210, 298, 925, 1228, 3074
HpyF10VI	GCNNNNNNNGC	6 cut(s)	533, 1145, 1512, 1786, 1992, 2156
HpyF3I	CTNAG	7 cut(s)	1223, 1284, 1743, 2612, 2760, 2849, 3152
HpySE526I	ACGT	7 cut(s)	60, 190, 210, 298, 925, 1228, 3074
Hsp92I	GRCGYC	2 cut(s)	502, 1228
HspAI	GCGC	6 cut(s)	723, 1402, 1729, 1986, 1993, 2840
KpnI	GGTACC	1 cut(s)	2580
Ksp22I	TGATCA	1 cut(s)	2685
LmnI	GCTCC	5 cut(s)	910, 1081, 1762, 1966, 2861
Lsp1109I	GCAGC	7 cut(s)	84, 457, 759, 1148, 2143, 2498, 3340
LweI	GCATC	6 cut(s)	34, 688, 1009, 1252, 1948, 3006
MaeI	CTAG	5 cut(s)	1271, 2052, 2241, 2372, 2399
MaeII	ACGT	7 cut(s)	60, 190, 210, 298, 925, 1228, 3074
MaeIII	GTNAC	6 cut(s)	261, 1003, 1440, 1703, 1980, 2086
MflI	RGATCY	4 cut(s)	808, 2056, 2938, 3265
MhlI	GDGCHC	5 cut(s)	1684, 2673, 2858, 2874, 3169
MlsI	TGGCCA	2 cut(s)	2651, 3189
MluNI	TGGCCA	2 cut(s)	2651, 3189
MlyI	GAGTC	2 cut(s)	2750, 2918
Mox20I	TGGCCA	2 cut(s)	2651, 3189
Mph1103I	ATGCAT	2 cut(s)	1267, 2511
MscI	TGGCCA	2 cut(s)	2651, 3189
MslI	CAYNNNNRTG	1 cut(s)	1386
Msp20I	TGGCCA	2 cut(s)	2651, 3189
MspA1I	CMGCKG	1 cut(s)	380
MspCI	CTTAAG	1 cut(s)	961
MspI	CCGG	9 cut(s)	101, 138, 696, 786, 1255, 1910, 2454, 2521, 2829
MspR9I	CCNGG	8 cut(s)	697, 786, 787, 794, 1255, 1566, 2454, 2876
MvaI	CCWGG	3 cut(s)	794, 1566, 2876
MvnI	CGCG	5 cut(s)	127, 725, 1010, 1654, 1986
MwoI	GCNNNNNNNGC	6 cut(s)	533, 1145, 1512, 1786, 1992, 2156
NciI	CCSGG	5 cut(s)	697, 786, 787, 1255, 2454
NdeI	CATATG	1 cut(s)	2620
NheI	GCTAGC	1 cut(s)	2398
NlaIV	GGNNCC	8 cut(s)	66, 791, 810, 1069, 1692, 1736, 1879, 2578
NmuCI	GTSAC	4 cut(s)	261, 1440, 1703, 2086
NsbI	TGCGCA	1 cut(s)	2841
NsiI	ATGCAT	2 cut(s)	1267, 2511
NspI	RCATGY	3 cut(s)	2196, 3290, 3302
NspV	TTCGAA	3 cut(s)	1373, 2634, 2796
PaeI	GCATGC	1 cut(s)	2196
PaeR7I	CTCGAG	1 cut(s)	588
PagI	TCATGA	4 cut(s)	319, 1036, 1381, 1447
PceI	AGGCCT	1 cut(s)	1033
PciI	ACATGT	1 cut(s)	3298
PcsI	WCGNNNNNNNCGW	1 cut(s)	80
PfeI	GAWTC	8 cut(s)	1592, 1999, 2005, 2317, 2362, 2375, 2798, 3008
PflFI	GACNNNGTC	1 cut(s)	2092
PflMI	CCANNNNNTGG	4 cut(s)	410, 562, 1801, 2027
PinAI	ACCGGT	1 cut(s)	1909
PkrI	GCNGC	9 cut(s)	99, 129, 472, 749, 1138, 1514, 2158, 2488, 3330
Ple19I	CGATCG	1 cut(s)	3201
PleI	GAGTC	2 cut(s)	2750, 2918
PpsI	GAGTC	2 cut(s)	2750, 2918
Ppu21I	YACGTR	1 cut(s)	211
PpuMI	RGGWCCY	1 cut(s)	2180
PscI	ACATGT	1 cut(s)	3298
PshAI	GACNNNNGTC	2 cut(s)	1104, 2736
PshBI	ATTAAT	1 cut(s)	2727
PsiI	TTATAA	1 cut(s)	1581
Psp1406I	AACGTT	1 cut(s)	3074
Psp5II	RGGWCCY	1 cut(s)	2180
Psp6I	CCWGG	3 cut(s)	792, 1564, 2874
PspEI	GGTNACC	1 cut(s)	1003
PspFI	CCCAGC	2 cut(s)	2234, 2532
PspGI	CCWGG	3 cut(s)	792, 1564, 2874
PspN4I	GGNNCC	8 cut(s)	66, 791, 810, 1069, 1692, 1736, 1879, 2578
PspPI	GGNCC	9 cut(s)	65, 129, 644, 1691, 1804, 1878, 2180, 2642, 2865
PspPPI	RGGWCCY	1 cut(s)	2180
PstNI	CAGNNNCTG	1 cut(s)	2492
PsuI	RGATCY	4 cut(s)	808, 2056, 2938, 3265
PsyI	GACNNNGTC	1 cut(s)	2092
PvuI	CGATCG	1 cut(s)	3201
RseI	CAYNNNNRTG	1 cut(s)	1386
SatI	GCNGC	9 cut(s)	98, 128, 471, 748, 1137, 1513, 2157, 2487, 3329
Sau96I	GGNCC	9 cut(s)	65, 129, 644, 1691, 1804, 1878, 2180, 2642, 2865
ScaI	AGTACT	1 cut(s)	3031
SchI	GAGTC	2 cut(s)	2750, 2918
ScrFI	CCNGG	8 cut(s)	697, 786, 787, 794, 1255, 1566, 2454, 2876
SduI	GDGCHC	5 cut(s)	1684, 2673, 2858, 2874, 3169
SfaNI	GCATC	6 cut(s)	34, 688, 1009, 1252, 1948, 3006
SfcI	CTRYAG	2 cut(s)	3139, 3230
Sfr274I	CTCGAG	1 cut(s)	588
SfuI	TTCGAA	3 cut(s)	1373, 2634, 2796
SgrAI	CRCCGGYG	1 cut(s)	1909
SinI	GGWCC	6 cut(s)	644, 1691, 1804, 1878, 2180, 2865
SlaI	CTCGAG	1 cut(s)	588
SmaI	CCCGGG	1 cut(s)	787
SmiMI	CAYNNNNRTG	1 cut(s)	1386
SmlI	CTYRAG	5 cut(s)	588, 961, 1938, 3019, 3381
SmoI	CTYRAG	5 cut(s)	588, 961, 1938, 3019, 3381
SphI	GCATGC	1 cut(s)	2196
SseBI	AGGCCT	1 cut(s)	1033
SspI	AATATT	1 cut(s)	2725
SspMI	CTAG	5 cut(s)	1271, 2052, 2241, 2372, 2399
StuI	AGGCCT	1 cut(s)	1033
StyD4I	CCNGG	8 cut(s)	695, 784, 785, 792, 1253, 1564, 2452, 2874
StyI	CCWWGG	4 cut(s)	1082, 1661, 2819, 3036
TaiI	ACGT	7 cut(s)	63, 193, 213, 301, 928, 1231, 3077
TaqI	TCGA	8 cut(s)	90, 589, 617, 1164, 1373, 2634, 2796, 3201
TatI	WGTACW	3 cut(s)	385, 2382, 3029
TauI	GCSGC	2 cut(s)	130, 1515
TfiI	GAWTC	8 cut(s)	1592, 1999, 2005, 2317, 2362, 2375, 2798, 3008
TscAI	CASTG	3 cut(s)	54, 1682, 2231
TseFI	GTSAC	4 cut(s)	261, 1440, 1703, 2086
TseI	GCWGC	7 cut(s)	97, 470, 747, 1136, 2156, 2486, 3328
Tsp45I	GTSAC	4 cut(s)	261, 1440, 1703, 2086
TspGWI	ACGGA	3 cut(s)	476, 1116, 2264
TspMI	CCCGGG	1 cut(s)	785
TspRI	CASTG	3 cut(s)	54, 1682, 2231
Tth111I	GACNNNGTC	1 cut(s)	2092
Van91I	CCANNNNNTGG	4 cut(s)	410, 562, 1801, 2027
Vha464I	CTTAAG	1 cut(s)	961
VneI	GTGCAC	1 cut(s)	2669
VpaK11BI	GGWCC	6 cut(s)	644, 1691, 1804, 1878, 2180, 2865
VspI	ATTAAT	1 cut(s)	2727
XapI	RAATTY	9 cut(s)	583, 602, 1428, 1603, 1891, 2074, 2199, 2834, 3060
XceI	RCATGY	3 cut(s)	2196, 3290, 3302
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2024, 2231
XhoI	CTCGAG	1 cut(s)	588
XmaI	CCCGGG	1 cut(s)	785
XspI	CTAG	5 cut(s)	1271, 2052, 2241, 2372, 2399
ZraI	GACGTC	1 cut(s)	1229
ZrmI	AGTACT	1 cut(s)	3031
Zsp2I	ATGCAT	2 cut(s)	1267, 2511

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.