Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0001577

Physical Location & Seq

Genomic Coordinates Reverse (-)

13252986 .. 13261031

Gene Structure 8046 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1162400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3327 bp

ATGGAATCGGACCAGTTAATCTCGGGCGGCGGGTCCCGCTACCTCCAAATGCGATCGGAGTCGGCCGTGTCGCCTTCCTCCTTTCTCTGCCGTCTTTCGTCCTTCGAGCCCGCTCGGATTTTCGATGAGTTGCCGAGCGCCACCATCGTCTCCGTCTCCCGTCCCGACGCCGGCGATATTAGCCCCGTGCTTCTCTCTTACACCATCGAGTTCCAATACAAGCAGTTTAAGTGGCGATTAGTGAAGAAACCGTCACATGTATTCTACTTGCATTTCGCATTGAAGAAACGTGCATTTTTCGAGGAAATTCAGGAGAAGCAAGAACAGGTTAGAGAATGGCTTCAAAATCTAGGAATAGGAGATCACACAGATGTGGTGCATGATCATGAGGATGATGAGACTGTTCCCCTGCAGCACGATGAAAGTGCTAAAAACAGAGATGTTCCATCTAGCGCCGCTCTTCCAATCATCCGGCCAGCGCTGGGTAGGGATCAATCCATTGCGGAGAGATCAAAGGTTGCAATGCAAGGGTATCTAAATCACTTTCTTGGAAACATGGACATTGTGAACTCTCGAGAGGTCTGCAAGTTCTTGGAGGTCTCGATGTTATCATTTTCTCCAGAATATGGCCCTAAGCTAAAAGAAGACTATGTGATGGTGAAGCATCTTCCGAAAATTCTTAAGGATGATAGTACCAGGAAATGTTGTGCATGTGGTTGGTTTAATTGCTGTAATGACAATTGGCAAAAGGTTTGGGCTGTATTAAAACCAGGATTCTTGGCCTTGCTGGCGCATCCTTTTGATACCCAACCTTTAGATATAATAGTATTTGATGTACTACCAGTCTCAGATGGAAATGGGGATGGCCAGGTATCACTGGCAAAAGAAATAAAAGAGCGGAATCCTTTACGTCATGCATTTAAGGTGGCTTGTGGAAACCGGAGCATTAATTTGAGAGTTAAGAGTGGTGCCAAAGTAAAAGATTGGGTTGCCTCAATCAATGATGCTGGACTCAGGCCTCCAGAGGGCTGGTGTCATCCTCACCGCTTTGGCTCTTTTGCACCTCCGAGAGGTTTGACTGAAGATGGTAGTCAGGTTCAATGGTTTATAGATGGTCAGTCAGCATTTGAAGCTATTGCGTCGGCAATTGAGGATGCAAAATCAGAGATATTTATTTGTGGCTGGTGGCTGTGTCCAGAACTGTACCTGCGGCGTCCATTTCATGCTCATGCTTCTTCTAGACTTGATTCTTTGCTGGAAGCAAAAGCTAAGGAAGGGGTTCAGATATACATTCTTCTCTATAAAGAGGTAGCTCTTGCTCTGAAAATCAACAGTGTTTATAGCAAGAGAAAACTACTTGGGATTCATGAGAATGTGAGAGTACTACGTTATCCTGACCACTTTTCAAGTGGTGTTTACTTATGGTCGCACCATGAAAAACTTGTCATTGTTGATTATCAGATTTGCTTTCTTGGAGGTCTGGATTTATGCTTTGGTCGTTACGATACTGCTGAGCATAAAGTGGGTGATTGTCCTCCTTCGATATGGCCTGGAAAGGACTATTACAACCCGAGGGAATCTGAACCAAATTCATGGGAGGATACAATGAAAGATGAGTTGGATCGTGGAAAATATCCTCGTATGCCCTGGCATGATGTTCACTGTGCCCTTTGGGGACCACCTTGCCGAGACGTGGCTAGACATTTTGTTCAGCGCTGGAACTATGCAAAGAGAAATAAGGCTCCAAACGAACAAGCAATTCCACTACTTATGCCGCAGCACCACATGGTCATTCCTCATTACATGGGAAGAAGCCAAGAGATGGAGATTGAAAGTAAGAACGCTAATAATCACAAAGAGCATAAAAGGACAGATTCCTTGTCGTCGATATCATCCTGTCAAGATATTCCGCTTCTTATACCCCAAGAAGCTGATGGGCTGGATTGTCCAAATGAAGACACAAAACTAAATGTGACAGACTCTCCTCATGATCTCCTTGATCAGCCAAGCAGGGTTAGCAACAATCTTGCTTTCCCTTTCCGGAAGTTAAAAATTGCACCAGTAGGTCCTGATACGCCCATGAGAGGCTTTGTAGATGACCTTGATTCTCTGGCTCGACATGGAAAAATGGCTTCTGACGGGGTGGCACAGCCTGGCATGAAGGATCCAGAGTGGTGGGAAACACAAGAAAGGGGCAACAAGGGTGGTTTCACAGATGAATCGGGACAAGTTGGCCCTTGTTGCTCATGTCGATGCCAGGTTATTAGAAGTGTCAGTCAGTGGTCAGCAGGAACAAGCCAAGTTGAAGAGAGCATTCACAATGCTTATTGCTCACTGATAGATAAGTCCGAACACTTCATTTACATTGAGAATCAATTTTTTATATCAGGTCTTTCAGGAGATGAAATTATACGGAATCGTGTTTTAGAAGCTTTATTTCGACGTATTATGCGAGCATACAATGATAAGAAGCCTTTTAGAGTTATAATTGTCATTCCGCTCATACCCGGCTTCCAGGGAGGTATGGATGATGCTGGTGCAGCATCTGTGAGAGCTGTTATGCATTGGCAATATCGAACCATTTGCAGAGGACAGAATTCAATATTGCACAATCTTAATGAAATTCTTGGTCCAAAGATGCATGATTACATTTCCTTCTATGGCCTTAGATCTTATGGTAAACTCTTTGAAGGCGGTCCTGTGGCCTGTAGTCAGGTCTATGTGCATAGTAAAATCATGATTGTCGATGATTGCACAACTTTAATTGGATCAGCTAACATCAATGATAGGAGTTTGCTTGGTTCAAGAGACTCTGAGATTGGTCTACTTATTGAAGATAAAGAGATGATTAATTCAGTTATGGGAGGAAAGCCATGGAAGGCTGGAAAGTTTTCATTGAGTCTTCGCTTGTCACTGTGGTCTGAACACCTTGGTATTCGTGCTACAGAGATGAGTCAAATAATCGACCCAATTGTTGATTCAACTTACAAGGACATCTGGATGGCAACTGCAAAGACAAATACCGCAATCTACCAAGATGTGTTTTATTGCATACCAAACGATTTTATACGGTCCAGAGCTGCATTCAGGCAAAGCATAGCCTTCTGGAAGGAGAGAATTGGACACACAACTATCGACTTGGGAATTGCTCCTGACCAGATAGAATCATTTCAAAATGGAGATGTGAAGAGAGCGAATCCCATGGAGAGGTTAGAGTCTGTGAAGGGCCATCTTGTTTCTTTCCCTTTGGATTTCATGTTGAAAGAAGATCTGAGACCTGTTTTCAACGAGAGCGAGTATTACGCGTCTCCTCAAGTTTTTCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004620 GO:0004630 GO:0006629 GO:0007275 GO:0008081 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0016298 GO:0016787 GO:0016788 GO:0022622 GO:0032501 GO:0032502 GO:0042578 GO:0044238 GO:0048364 GO:0048731 GO:0048856 GO:0071704 GO:0099402 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

PLDc PLDc_2

Protein Analysis

1109

Amino Acids

125.72

Weight (kDa)

6.29

Isoelectric Point (pI)

48.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PLDc	PF00614	473 - 500	3.9e-06	Phospholipase D Active site motif
PLDc_2	PF13091	892 - 951	3.6e-08	PLD-like domain
PLDc	PF00614	908 - 931	2.5e-06	Phospholipase D Active site motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001577)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16785 AT3G16785 AT3G16785 AT3G16785 AT3G16785 AT3G16785
fragaria_vesca	FvH4_4g18810 FvH4_6g06940 FvH4_6g06940 FvH4_6g06940
malus_domestica	MD04G1197500.v1.1 MD12G1210300.v1.1 MD13G1162600.v1.1 MD16G1162400.v1.1
prunus_persica	Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.1G130000_v2.0.a1 Prupe.6G317900_v2.0.a1 Prupe.6G317900_v2.0.a1 Prupe.6G317900_v2.0.a1
pyrus_communis	pycom04g17480 pycom13g13890 pycom16g13770
rosa_chinensis	RchiOBHm_Chr3g0456481 RchiOBHm_Chr4g0423621
rosa_laevigata	RLG00000007541 RLG00000025302
rosa_multiflora	Rmu_co8413375.1_g000001 Rmu_sc0000464.1_g000017 Rmu_sc0002607.1_g000011 Rmu_sc0008373.1_g000012 Rmu_sc0016309.1_g000001
rosa_roxburghii	Rroxscaffold_5G00365830 Rroxscaffold_6G00422720
rosa_rugosa	Rorug03G0013400 Rorug03G0013400 Rorug03G0013400 Rorug04G0189100
rosa_samantha	Rh3AG073700 Rh3BG075600 Rh3DG076900 Rh4AG248700 Rh4BG254000 Rh4DG249000
rosa_wichuraiana	Rw3G006320 Rw4G021300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2486
Acc36I	ACCTGC	1 cut(s)	1215
AccB1I	GGYRCC	1 cut(s)	968
AccB7I	CCANNNNNTGG	2 cut(s)	626, 1822
AccBSI	CCGCTC	4 cut(s)	113, 458, 898, 2500
AccI	GTMKAC	1 cut(s)	2824
AccII	CGCG	1 cut(s)	3305
AccIII	TCCGGA	1 cut(s)	2040
AclWI	GGATC	5 cut(s)	498, 1629, 2159, 2172, 2776
AcoI	YGGCCR	3 cut(s)	63, 473, 865
AcsI	RAATTY	5 cut(s)	306, 675, 1588, 2596, 2622
AcuI	CTGAAG	1 cut(s)	1101
AcyI	GRCGYC	2 cut(s)	168, 1213
AfaI	GTAC	4 cut(s)	694, 837, 1205, 1383
AfeI	AGCGCT	2 cut(s)	480, 1715
AfiI	CCNNNNNNNGG	9 cut(s)	170, 482, 626, 1025, 1070, 1693, 1822, 2084, 3207
AflII	CTTAAG	1 cut(s)	680
AflIII	ACRYGT	2 cut(s)	256, 3303
AjiI	CACGTC	1 cut(s)	1693
AjnI	CCWGG	8 cut(s)	695, 769, 867, 1549, 1646, 2152, 2256, 2514
AjuI	GAANNNNNNNTTGG	4 cut(s)	1601, 1633, 2962, 2994
AleI	CACNNNNGTG	1 cut(s)	371
AluBI	AGCT	9 cut(s)	637, 1133, 1268, 1313, 1931, 2432, 2555, 2774, 3080
AluI	AGCT	9 cut(s)	637, 1133, 1268, 1313, 1931, 2432, 2555, 2774, 3080
Alw26I	GTCTC	9 cut(s)	154, 160, 392, 604, 850, 1683, 2802, 3268, 3312
AlwI	GGATC	5 cut(s)	498, 1629, 2159, 2172, 2776
AlwNI	CAGNNNCTG	1 cut(s)	2546
Ama87I	CYCGRG	3 cut(s)	22, 573, 1570
Aor13HI	TCCGGA	1 cut(s)	2040
Aor51HI	AGCGCT	2 cut(s)	480, 1715
ApeKI	GCWGC	4 cut(s)	412, 1777, 2540, 3080
ApoI	RAATTY	5 cut(s)	306, 675, 1588, 2596, 2622
AseI	ATTAAT	2 cut(s)	948, 2850
Asp700I	GAANNNNTTC	4 cut(s)	339, 1278, 2890, 3168
AspLEI	GCGC	5 cut(s)	140, 455, 481, 793, 1716
AsuC2I	CCSGG	1 cut(s)	2508
AsuHPI	GGTGA	3 cut(s)	670, 1034, 1538
AvaI	CYCGRG	3 cut(s)	22, 573, 1570
AvaII	GGWCC	7 cut(s)	10, 33, 1676, 2066, 2630, 2696, 3072
BaeGI	GKGCMC	1 cut(s)	1669
BalI	TGGCCA	1 cut(s)	867
BamHI	GGATCC	1 cut(s)	2164
BanI	GGYRCC	1 cut(s)	968
BanII	GRGCYC	1 cut(s)	111
BbsI	GAAGAC	3 cut(s)	651, 1962, 2894
BbvI	GCAGC	4 cut(s)	424, 1789, 2552, 3067
BceAI	ACGGC	2 cut(s)	50, 75
BcgI	CGANNNNNNTGC	6 cut(s)	407, 441, 564, 598, 2565, 2599
BciT130I	CCWGG	8 cut(s)	697, 771, 869, 1551, 1648, 2154, 2258, 2516
BciVI	GTATCC	1 cut(s)	1594
BclI	TGATCA	2 cut(s)	382, 1999
BcnI	CCSGG	1 cut(s)	2508
BcoDI	GTCTC	9 cut(s)	154, 160, 392, 604, 850, 1683, 2802, 3268, 3312
BfaI	CTAG	4 cut(s)	350, 450, 1239, 1698
BfmI	CTRYAG	3 cut(s)	410, 2707, 2943
BfoI	RGCGCY	4 cut(s)	141, 456, 482, 1717
BfrI	CTTAAG	1 cut(s)	680
BfuAI	ACCTGC	1 cut(s)	1215
BfuI	GTATCC	1 cut(s)	1594
BglI	GCCNNNNNGGC	1 cut(s)	788
BglII	AGATCT	2 cut(s)	2669, 3268
BisI	GCNGC	8 cut(s)	28, 413, 456, 1211, 1775, 1778, 2541, 3081
BlpI	GCTNAGC	1 cut(s)	1512
BlsI	GCNGC	8 cut(s)	29, 414, 457, 1212, 1776, 1779, 2542, 3082
BmcAI	AGTACT	1 cut(s)	1383
Bme1390I	CCNGG	9 cut(s)	697, 771, 869, 1551, 1648, 2154, 2258, 2508, 2516
Bme18I	GGWCC	7 cut(s)	10, 33, 1676, 2066, 2630, 2696, 3072
BmeT110I	CYCGRG	3 cut(s)	22, 573, 1570
BmgBI	CACGTC	1 cut(s)	1693
BmiI	GGNNCC	6 cut(s)	34, 35, 970, 1677, 1743, 2166
BmrFI	CCNGG	9 cut(s)	697, 771, 869, 1551, 1648, 2154, 2258, 2508, 2516
BmsI	GCATC	8 cut(s)	673, 802, 994, 1144, 2243, 2521, 2552, 2628
BpiI	GAAGAC	3 cut(s)	651, 1962, 2894
BpmI	CTGGAG	2 cut(s)	603, 1005
Bpu10I	CCTNAGC	2 cut(s)	633, 1269
Bpu1102I	GCTNAGC	1 cut(s)	1512
BpuEI	CTTGAG	1 cut(s)	3297
BpuMI	CCSGG	1 cut(s)	2508
BsaHI	GRCGYC	2 cut(s)	168, 1213
BsaI	GGTCTC	2 cut(s)	604, 3268
BsaJI	CCNNGG	6 cut(s)	1571, 1646, 2515, 2873, 2929, 3201
BsaWI	WCCGGW	2 cut(s)	939, 2040
BsaXI	ACNNNNNCTCC	2 cut(s)	1966, 1996
Bsc4I	CCNNNNNNNGG	9 cut(s)	170, 482, 626, 1025, 1070, 1693, 1822, 2084, 3207
Bse118I	RCCGGY	1 cut(s)	170
Bse1I	ACTGG	4 cut(s)	13, 842, 882, 2060
Bse3DI	GCAATG	2 cut(s)	498, 528
BseAI	TCCGGA	1 cut(s)	2040
BseBI	CCWGG	8 cut(s)	697, 771, 869, 1551, 1648, 2154, 2258, 2516
BseDI	CCNNGG	6 cut(s)	1571, 1646, 2515, 2873, 2929, 3201
BseLI	CCNNNNNNNGG	9 cut(s)	170, 482, 626, 1025, 1070, 1693, 1822, 2084, 3207
BseMI	GCAATG	2 cut(s)	498, 528
BseMII	CTCAG	5 cut(s)	861, 1027, 1503, 2805, 3263
BseNI	ACTGG	4 cut(s)	13, 842, 882, 2060
BseRI	GAGGAG	2 cut(s)	1974, 3300
BseSI	GKGCMC	1 cut(s)	1669
BseX3I	CGGCCG	1 cut(s)	63
BseXI	GCAGC	4 cut(s)	424, 1789, 2552, 3067
BseYI	CCCAGC	1 cut(s)	481
BsgI	GTGCAG	1 cut(s)	2559
Bsh1236I	CGCG	1 cut(s)	3305
Bsh1285I	CGRYCG	2 cut(s)	56, 66
BshNI	GGYRCC	1 cut(s)	968
BsiEI	CGRYCG	2 cut(s)	56, 66
BsiHKCI	CYCGRG	3 cut(s)	22, 573, 1570
BsiSI	CCGG	5 cut(s)	171, 472, 940, 2041, 2508
BslFI	GGGAC	4 cut(s)	19, 147, 1689, 2238
BslI	CCNNNNNNNGG	9 cut(s)	170, 482, 626, 1025, 1070, 1693, 1822, 2084, 3207
BsmAI	GTCTC	9 cut(s)	154, 160, 392, 604, 850, 1683, 2802, 3268, 3312
BsmBI	CGTCTC	4 cut(s)	154, 160, 1683, 3312
BsmFI	GGGAC	4 cut(s)	19, 147, 1689, 2238
BsmI	GAATGC	2 cut(s)	2313, 3083
Bso31I	GGTCTC	2 cut(s)	604, 3268
BsoBI	CYCGRG	3 cut(s)	22, 573, 1570
Bsp1286I	GDGCHC	2 cut(s)	111, 1669
Bsp13I	TCCGGA	1 cut(s)	2040
Bsp1720I	GCTNAGC	1 cut(s)	1512
Bsp19I	CCATGG	2 cut(s)	2873, 3201
BspCNI	CTCAG	5 cut(s)	860, 1026, 1504, 2806, 3264
BspEI	TCCGGA	1 cut(s)	2040
BspFNI	CGCG	1 cut(s)	3305
BspHI	TCATGA	4 cut(s)	385, 1366, 1987, 2736
BspLI	GGNNCC	6 cut(s)	34, 35, 970, 1677, 1743, 2166
BspMAI	CTGCAG	1 cut(s)	414
BspMI	ACCTGC	1 cut(s)	1215
BspPI	GGATC	5 cut(s)	498, 1629, 2159, 2172, 2776
BspQI	GCTCTTC	1 cut(s)	465
BspT107I	GGYRCC	1 cut(s)	968
BspTI	CTTAAG	1 cut(s)	680
BspTNI	GGTCTC	2 cut(s)	604, 3268
BsrBI	CCGCTC	4 cut(s)	113, 458, 898, 2500
BsrDI	GCAATG	2 cut(s)	498, 528
BsrFI	RCCGGY	1 cut(s)	170
BsrI	ACTGG	4 cut(s)	13, 842, 882, 2060
BssAI	RCCGGY	1 cut(s)	170
BssECI	CCNNGG	6 cut(s)	1571, 1646, 2515, 2873, 2929, 3201
BssNI	GRCGYC	2 cut(s)	168, 1213
BssT1I	CCWWGG	3 cut(s)	2873, 2929, 3201
Bst2UI	CCWGG	8 cut(s)	697, 771, 869, 1551, 1648, 2154, 2258, 2516
Bst4CI	ACNGT	7 cut(s)	252, 403, 1203, 1334, 1664, 2916, 3072
Bst6I	CTCTTC	3 cut(s)	465, 2301, 3182
BstACI	GRCGYC	2 cut(s)	168, 1213
BstAFI	CTTAAG	1 cut(s)	680
BstC8I	GCNNGC	5 cut(s)	111, 172, 477, 789, 2454
BstDEI	CTNAG	8 cut(s)	633, 847, 1013, 1269, 1512, 2666, 2814, 3272
BstDSI	CCRYGG	2 cut(s)	2873, 3201
BstENI	CCTNNNNNAGG	1 cut(s)	1068
BstFNI	CGCG	1 cut(s)	3305
BstH2I	RGCGCY	4 cut(s)	141, 456, 482, 1717
BstHHI	GCGC	5 cut(s)	140, 455, 481, 793, 1716
BstMAI	GTCTC	9 cut(s)	154, 160, 392, 604, 850, 1683, 2802, 3268, 3312
BstMCI	CGRYCG	2 cut(s)	56, 66
BstMWI	GCNNNNNNNGC	7 cut(s)	36, 180, 788, 1130, 2016, 2241, 2540
BstNI	CCWGG	8 cut(s)	697, 771, 869, 1551, 1648, 2154, 2258, 2516
BstNSI	RCATGY	2 cut(s)	260, 714
BstSCI	CCNGG	9 cut(s)	695, 769, 867, 1549, 1646, 2152, 2256, 2506, 2514
BstSFI	CTRYAG	3 cut(s)	410, 2707, 2943
BstSLI	GKGCMC	1 cut(s)	1669
BstUI	CGCG	1 cut(s)	3305
BstV1I	GCAGC	4 cut(s)	424, 1789, 2552, 3067
BstV2I	GAAGAC	3 cut(s)	651, 1962, 2894
BstX2I	RGATCY	3 cut(s)	2164, 2669, 3268
BstXI	CCANNNNNNTGG	3 cut(s)	1029, 1593, 2175
BstYI	RGATCY	3 cut(s)	2164, 2669, 3268
BstZI	CGGCCG	1 cut(s)	63
BsuI	GTATCC	1 cut(s)	1594
BtgI	CCRYGG	2 cut(s)	2873, 3201
BtrI	CACGTC	1 cut(s)	1693
BtsIMutI	CAGTG	6 cut(s)	875, 1339, 1660, 2285, 2333, 2912
BveI	ACCTGC	1 cut(s)	1215
Cac8I	GCNNGC	5 cut(s)	111, 172, 477, 789, 2454
CaiI	CAGNNNCTG	1 cut(s)	2546
CciI	TCATGA	4 cut(s)	385, 1366, 1987, 2736
CfoI	GCGC	5 cut(s)	140, 455, 481, 793, 1716
Cfr10I	RCCGGY	1 cut(s)	170
CseI	GACGC	4 cut(s)	176, 1128, 1202, 3294
Csp6I	GTAC	4 cut(s)	693, 836, 1204, 1382
CspCI	CAANNNNNGTGG	2 cut(s)	2185, 2220
CviQI	GTAC	4 cut(s)	693, 836, 1204, 1382
DdeI	CTNAG	8 cut(s)	633, 847, 1013, 1269, 1512, 2666, 2814, 3272
EaeI	YGGCCR	3 cut(s)	63, 473, 865
EagI	CGGCCG	1 cut(s)	63
Eam1104I	CTCTTC	3 cut(s)	465, 2301, 3182
EarI	CTCTTC	3 cut(s)	465, 2301, 3182
EclXI	CGGCCG	1 cut(s)	63
Eco130I	CCWWGG	3 cut(s)	2873, 2929, 3201
Eco147I	AGGCCT	1 cut(s)	1018
Eco24I	GRGCYC	1 cut(s)	111
Eco31I	GGTCTC	2 cut(s)	604, 3268
Eco32I	GATATC	1 cut(s)	1890
Eco47I	GGWCC	7 cut(s)	10, 33, 1676, 2066, 2630, 2696, 3072
Eco47III	AGCGCT	2 cut(s)	480, 1715
Eco52I	CGGCCG	1 cut(s)	63
Eco57I	CTGAAG	1 cut(s)	1101
Eco88I	CYCGRG	3 cut(s)	22, 573, 1570
EcoNI	CCTNNNNNAGG	1 cut(s)	1068
EcoO109I	RGGNCCY	2 cut(s)	33, 2066
EcoRI	GAATTC	1 cut(s)	2596
EcoRII	CCWGG	8 cut(s)	695, 769, 867, 1549, 1646, 2152, 2256, 2514
EcoRV	GATATC	1 cut(s)	1890
EcoT14I	CCWWGG	3 cut(s)	2873, 2929, 3201
EcoT22I	ATGCAT	3 cut(s)	919, 2565, 2643
EcoT38I	GRGCYC	1 cut(s)	111
ErhI	CCWWGG	3 cut(s)	2873, 2929, 3201
Esp3I	CGTCTC	4 cut(s)	154, 160, 1683, 3312
FaqI	GGGAC	4 cut(s)	19, 147, 1689, 2238
FauI	CCCGC	3 cut(s)	23, 44, 118
FbaI	TGATCA	2 cut(s)	382, 1999
FblI	GTMKAC	1 cut(s)	2824
Fnu4HI	GCNGC	8 cut(s)	28, 413, 456, 1211, 1775, 1778, 2541, 3081
FriOI	GRGCYC	1 cut(s)	111
Fsp4HI	GCNGC	8 cut(s)	28, 413, 456, 1211, 1775, 1778, 2541, 3081
FspBI	CTAG	4 cut(s)	350, 450, 1239, 1698
GlaI	GCGC	5 cut(s)	139, 454, 480, 792, 1715
GluI	GCNGC	8 cut(s)	28, 413, 456, 1211, 1775, 1778, 2541, 3081
GsaI	CCCAGC	1 cut(s)	485
GsuI	CTGGAG	2 cut(s)	603, 1005
HaeII	RGCGCY	4 cut(s)	141, 456, 482, 1717
HapII	CCGG	5 cut(s)	171, 472, 940, 2041, 2508
HgaI	GACGC	4 cut(s)	176, 1128, 1202, 3294
HhaI	GCGC	5 cut(s)	140, 455, 481, 793, 1716
Hin1I	GRCGYC	2 cut(s)	168, 1213
Hin6I	GCGC	5 cut(s)	138, 453, 479, 791, 1714
HinP1I	GCGC	5 cut(s)	138, 453, 479, 791, 1714
HindIII	AAGCTT	1 cut(s)	2430
HpaII	CCGG	5 cut(s)	171, 472, 940, 2041, 2508
HphI	GGTGA	3 cut(s)	670, 1034, 1538
Hpy166II	GTNNAC	5 cut(s)	568, 1417, 1660, 2681, 2825
Hpy8I	GTNNAC	5 cut(s)	568, 1417, 1660, 2681, 2825
Hpy99I	CGWCG	4 cut(s)	170, 1144, 1888, 2445
HpyCH4III	ACNGT	7 cut(s)	252, 403, 1203, 1334, 1664, 2916, 3072
HpyCH4IV	ACGT	5 cut(s)	289, 910, 1387, 1692, 2443
HpyF10VI	GCNNNNNNNGC	7 cut(s)	36, 180, 788, 1130, 2016, 2241, 2540
HpyF3I	CTNAG	8 cut(s)	633, 847, 1013, 1269, 1512, 2666, 2814, 3272
HpySE526I	ACGT	5 cut(s)	289, 910, 1387, 1692, 2443
Hsp92I	GRCGYC	2 cut(s)	168, 1213
HspAI	GCGC	5 cut(s)	138, 453, 479, 791, 1714
KflI	GGGWCCC	1 cut(s)	33
Kpn2I	TCCGGA	1 cut(s)	2040
KroI	GCCGGC	1 cut(s)	170
KroNI	GCCGGC	1 cut(s)	172
Ksp22I	TGATCA	2 cut(s)	382, 1999
LguI	GCTCTTC	1 cut(s)	465
LmnI	GCTCC	3 cut(s)	942, 1747, 3154
Lsp1109I	GCAGC	4 cut(s)	424, 1789, 2552, 3067
LweI	GCATC	8 cut(s)	673, 802, 994, 1144, 2243, 2521, 2552, 2628
MaeI	CTAG	4 cut(s)	350, 450, 1239, 1698
MaeII	ACGT	5 cut(s)	289, 910, 1387, 1692, 2443
MaeIII	GTNAC	4 cut(s)	252, 1499, 1972, 2910
MbiI	CCGCTC	4 cut(s)	113, 458, 898, 2500
MfeI	CAATTG	3 cut(s)	739, 1146, 2970
MflI	RGATCY	3 cut(s)	2164, 2669, 3268
MhlI	GDGCHC	2 cut(s)	111, 1669
MlsI	TGGCCA	1 cut(s)	867
MluI	ACGCGT	1 cut(s)	3303
MluNI	TGGCCA	1 cut(s)	867
MlyI	GAGTC	7 cut(s)	68, 1005, 1973, 2804, 2908, 2962, 3224
MmeI	TCCRAC	1 cut(s)	1599
Mox20I	TGGCCA	1 cut(s)	867
Mph1103I	ATGCAT	3 cut(s)	919, 2565, 2643
MreI	CGCCGGCG	1 cut(s)	170
MroI	TCCGGA	1 cut(s)	2040
MroNI	GCCGGC	1 cut(s)	170
MroXI	GAANNNNTTC	4 cut(s)	339, 1278, 2890, 3168
MscI	TGGCCA	1 cut(s)	867
MslI	CAYNNNNRTG	8 cut(s)	369, 371, 384, 390, 1227, 1371, 2251, 2999
Msp20I	TGGCCA	1 cut(s)	867
MspCI	CTTAAG	1 cut(s)	680
MspI	CCGG	5 cut(s)	171, 472, 940, 2041, 2508
MspR9I	CCNGG	9 cut(s)	697, 771, 869, 1551, 1648, 2154, 2258, 2508, 2516
MunI	CAATTG	3 cut(s)	739, 1146, 2970
Mva1269I	GAATGC	2 cut(s)	2313, 3083
MvaI	CCWGG	8 cut(s)	697, 771, 869, 1551, 1648, 2154, 2258, 2516
MvnI	CGCG	1 cut(s)	3305
MwoI	GCNNNNNNNGC	7 cut(s)	36, 180, 788, 1130, 2016, 2241, 2540
NaeI	GCCGGC	1 cut(s)	172
NciI	CCSGG	1 cut(s)	2508
NcoI	CCATGG	2 cut(s)	2873, 3201
NgoMIV	GCCGGC	1 cut(s)	170
NlaIV	GGNNCC	6 cut(s)	34, 35, 970, 1677, 1743, 2166
NmeAIII	GCCGAG	2 cut(s)	159, 1712
NmuCI	GTSAC	3 cut(s)	252, 1972, 2910
NsiI	ATGCAT	3 cut(s)	919, 2565, 2643
NspI	RCATGY	2 cut(s)	260, 714
OliI	CACNNNNGTG	1 cut(s)	371
PaeR7I	CTCGAG	1 cut(s)	573
PagI	TCATGA	4 cut(s)	385, 1366, 1987, 2736
PceI	AGGCCT	1 cut(s)	1018
PciI	ACATGT	1 cut(s)	256
PciSI	GCTCTTC	1 cut(s)	465
PcsI	WCGNNNNNNNCGW	1 cut(s)	2449
PctI	GAATGC	2 cut(s)	2313, 3083
PdiI	GCCGGC	1 cut(s)	172
PdmI	GAANNNNTTC	4 cut(s)	339, 1278, 2890, 3168
PflMI	CCANNNNNTGG	2 cut(s)	626, 1822
PkrI	GCNGC	8 cut(s)	29, 414, 457, 1212, 1776, 1779, 2542, 3082
Ple19I	CGATCG	1 cut(s)	56
PleI	GAGTC	7 cut(s)	67, 1005, 1973, 2804, 2907, 2961, 3223
PpsI	GAGTC	7 cut(s)	67, 1005, 1973, 2804, 2907, 2961, 3223
PpuMI	RGGWCCY	2 cut(s)	33, 2066
PscI	ACATGT	1 cut(s)	256
PshBI	ATTAAT	2 cut(s)	948, 2850
PsiI	TTATAA	1 cut(s)	2486
Psp5II	RGGWCCY	2 cut(s)	33, 2066
Psp6I	CCWGG	8 cut(s)	695, 769, 867, 1549, 1646, 2152, 2256, 2514
PspFI	CCCAGC	1 cut(s)	481
PspGI	CCWGG	8 cut(s)	695, 769, 867, 1549, 1646, 2152, 2256, 2514
PspN4I	GGNNCC	6 cut(s)	34, 35, 970, 1677, 1743, 2166
PspPPI	RGGWCCY	2 cut(s)	33, 2066
PstI	CTGCAG	1 cut(s)	414
PstNI	CAGNNNCTG	1 cut(s)	2546
PsuI	RGATCY	3 cut(s)	2164, 2669, 3268
PvuI	CGATCG	1 cut(s)	56
RsaI	GTAC	4 cut(s)	694, 837, 1205, 1383
RsaNI	GTAC	4 cut(s)	693, 836, 1204, 1382
RseI	CAYNNNNRTG	8 cut(s)	369, 371, 384, 390, 1227, 1371, 2251, 2999
SapI	GCTCTTC	1 cut(s)	465
SatI	GCNGC	8 cut(s)	28, 413, 456, 1211, 1775, 1778, 2541, 3081
ScaI	AGTACT	1 cut(s)	1383
SchI	GAGTC	7 cut(s)	68, 1005, 1973, 2804, 2908, 2962, 3224
ScrFI	CCNGG	9 cut(s)	697, 771, 869, 1551, 1648, 2154, 2258, 2508, 2516
SduI	GDGCHC	2 cut(s)	111, 1669
SfaNI	GCATC	8 cut(s)	673, 802, 994, 1144, 2243, 2521, 2552, 2628
SfcI	CTRYAG	3 cut(s)	410, 2707, 2943
Sfr274I	CTCGAG	1 cut(s)	573
SgrAI	CRCCGGYG	1 cut(s)	170
SinI	GGWCC	7 cut(s)	10, 33, 1676, 2066, 2630, 2696, 3072
SlaI	CTCGAG	1 cut(s)	573
SmiMI	CAYNNNNRTG	8 cut(s)	369, 371, 384, 390, 1227, 1371, 2251, 2999
SmlI	CTYRAG	3 cut(s)	573, 680, 3312
SmoI	CTYRAG	3 cut(s)	573, 680, 3312
SseBI	AGGCCT	1 cut(s)	1018
SspI	AATATT	1 cut(s)	2604
SspMI	CTAG	4 cut(s)	350, 450, 1239, 1698
StuI	AGGCCT	1 cut(s)	1018
StyD4I	CCNGG	9 cut(s)	695, 769, 867, 1549, 1646, 2152, 2256, 2506, 2514
StyI	CCWWGG	3 cut(s)	2873, 2929, 3201
TaaI	ACNGT	7 cut(s)	252, 403, 1203, 1334, 1664, 2916, 3072
TaiI	ACGT	5 cut(s)	292, 913, 1390, 1695, 2446
TatI	WGTACW	2 cut(s)	835, 1381
TauI	GCSGC	4 cut(s)	30, 458, 1213, 1777
TscAI	CASTG	6 cut(s)	882, 1339, 1667, 2285, 2340, 2919
TseFI	GTSAC	3 cut(s)	252, 1972, 2910
TseI	GCWGC	4 cut(s)	412, 1777, 2540, 3080
Tsp45I	GTSAC	3 cut(s)	252, 1972, 2910
TspGWI	ACGGA	2 cut(s)	142, 2428
TspRI	CASTG	6 cut(s)	882, 1339, 1667, 2285, 2340, 2919
Van91I	CCANNNNNTGG	2 cut(s)	626, 1822
Vha464I	CTTAAG	1 cut(s)	680
VpaK11BI	GGWCC	7 cut(s)	10, 33, 1676, 2066, 2630, 2696, 3072
VspI	ATTAAT	2 cut(s)	948, 2850
XagI	CCTNNNNNAGG	1 cut(s)	1068
XapI	RAATTY	5 cut(s)	306, 675, 1588, 2596, 2622
XbaI	TCTAGA	1 cut(s)	1238
XceI	RCATGY	2 cut(s)	260, 714
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1406, 1931
XhoI	CTCGAG	1 cut(s)	573
XmiI	GTMKAC	1 cut(s)	2824
XmnI	GAANNNNTTC	4 cut(s)	339, 1278, 2890, 3168
XspI	CTAG	4 cut(s)	350, 450, 1239, 1698
ZrmI	AGTACT	1 cut(s)	1383
Zsp2I	ATGCAT	3 cut(s)	919, 2565, 2643

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.