MD04G1204000.v1.1

Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000763
Physical Location & Seq
Reverse (-)
29011559 .. 29015516
3958 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1204000.v1.1.491

Sequence Viewer

Length: 2607 bp
ATGCTGCATTCCAAGTATCCCACATCGTCCCAGGATACCACCGTTGATACGCAACACAAAAATGAGGGCAAGAAGATCAAGGGGAGAGTGGTGTTGATGAAGAAGGTTGTTCTCGATGTGAACGACCTTAAAGCTTCGATTCTTGATCGTGTTGACGAGCTGTTAGGCAAAGCAGTTTCTCTAAGGCTCATTAGTTCGGTTAATGGTGCCCCTGAAAATGAGTTGAAAGGAAAAGTTGGAAAGCCAGCATACTTGGAGAACTGGGTGACCACAATCGCCCCCTTAACAGCAGGGGAGGCTGCATTTGATGTTACTTTTGATTGGGAAAAGGAAATTGGCGTCCCGGGAGCTTTCGTAGTTAGAAATGAGCATCACAGTGAATTCTACTTGAAGACTCTCACGCTCGAAGATGTTCCTGGCGAAGGTCGAGTCCACTTTGTTTGCAACTCATGGGTGTATCCTGCTGACAAGTACAAAAAAGACCGTGTTTTCTTCTCTAACAAGACTTATCTTTCAAGTGATACACCAAAGCCACTACAGAAATTTAGAGAAGAAGAGCTAGTAAACCTGCGAGGAGACGATGAAGAAAGAGGAGAGCTTCAGGAATGGGACAGGGTCTATGACTATGCATACTACAACGATTTGGGGAATCCAGATAAGGGTCCCGAATATGCACGTCCAGTTCTTGGAGGATCTATTGAGTACCCATATCCTCGCCGCGGAAAAACAGGAAGACCAGCAACAAAAACAGATCCTAACACCGAGAGCAGGCTGAAGCTTATCCAGATCTTAAATGTATACGTTCCAAGAGACGAACGATTTGGACCCATAAAGATGTCAGACTTGCTTGCTTATGTCCTGAAATCCATCCCTCAGGTCCTTAAACCTGAGATAAGAGATCTACTCGTTGGTAACAAAGACGAGTTCGAAAGCATGGAAGAAGTACTTAAACTCTATGAAGGAGGATTAGAGTTGCCTGATGGGATACTAAAATACATTAGTGATAGCACACCTGGGGAGATTTTCAAGGAGCTTTTTCGAACTGATGGCGAAAAGTTCCTAAAATTTCCAGTGCCTCAAGTGATCAAAGAGGATAAGTCGGCATGGAGAACTGACGAAGAATTTGCAAGAGAACTGCTGGCTGGAGTAAATCCTGTCACTATACGTCGTCTCCAAGAATTTCCACCGTCTAGCGAGCTAGACCGAGAAGTATATGGGGATCAAACAAGTCAAATAACCAAAGAACACATAGAACATAACTTAAATGGACTCACCATAGATGAGGCAATCATGAACAACAAGCTATTCATATTAAACCACCACGATGCATGGATGCCGTACCTGAGGCGGATAAACAGAACTTCCACAAAAGCTTACGCAAGCAGAACACTCCTTTTCTTGAACAATGATGGGACTTTGAAGCCAATAGCTATTGAACTCAGCTTGCCACATCCGGATGGAGATCAATTTGGTTGCATTAGCAAAGTCTATACTCCGTCTAGCCAAGGCGTCGAGAGTTCCATTTGGCAATTTGCTAAAGGTTATGCACTTGTAAATGACTCCGGCTGTCATCAGCTTTTCAGCCACTGGTTGAGAGCTCATGCAGTGACTGAGCCATTTGTGATAGCGGCAAATAGGCAGTTGAGCGTGCTTCACCCGATTCACAAACTTCTGCATCCTCATTTTCGTGGGACGATGAATGCAAACGCATCCGCACGACAGGTTCTCACAAACGCAGGTGGAGTAATAGAGGAAATAATATTTGCTTCCAAGTTTTCGATGGAATGGTCATCAGCAATGTACAAGGACTGGACTTTTCCTGATCAAGCGCTCCCTGCGGATCTGATCAAAAGAGGAGTGGCGGTTGAAGATTCAAGTGCATCACACGGCGTGCGTCTACTGATAGAGGACTATCCATATGCTGCGGACGGGCTTGAATTATGGACTGCAATTAAAACATGGGTTAAAGACTATTGCTCCTTCTACTACAAAAATGATCAAATGGTTCAAAATGATTCGGAACTCCAATCCTGGTGGAAGGAACTGCGCGGGGTAGGCCACAGCGACAAGAAAGAAGAACCCTGGTGGCCTAAAATGCAGACTTGTGATGAGCTGATAGAATCATGCACAACTATCATATATATTGCTTCAGCCCATCATGCAGCAATCAACTACGGTCAGTACTCGACTGGTGGATTCGTCCCAAACCGCCCAACCCTAAGCCGGCGGTTCATGCCTGAAGAAGGCACTCCAGAGTATGAGGAGCTCAGGACAAACCCTGACAAGGCATTCTTGCAAACGTTTGCACCTCAGCTGCCGACACTTCTTGGAATGGCCACGGTAGAAATCTTGTCAAGGCACCCCACTGATGAGCTCTATCTCGGACAGCGTGACACCGCGGAGTGGACAACGGATGCAGACATACTGCAGGCTTCTGAGGATTTTAAAAAGAATTTGGAAGCGATTGAGGCCAAAATTGAAAAGATGAACAAGGATGAGAGATTGAAAAACCGGTTCGGACCGGCCAAGATCCCTTACACTTTGCTGTATCCTTCTAGTGAGCCTGGACTTACTAACAAGGGAGTTCCCACCAGTATCAACATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00591 map00591
Pfam Domains
Protein Families
Lipoxygenase PLAT

Protein Analysis

869

Amino Acids

98.71

Weight (kDa)

5.59

Isoelectric Point (pI)

43.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAT PF01477 62 - 165 1.3e-22 PLAT/LH2 domain
Lipoxygenase PF00305 178 - 851 0.0 Lipoxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1727
Acc36I ACCTGC 2 cut(s) 576, 1727
AccB1I GGYRCC 2 cut(s) 206, 2358
AccB7I CCANNNNNTGG 1 cut(s) 686
AccI GTMKAC 2 cut(s) 798, 1897
AccII CGCG 3 cut(s) 720, 2049, 2399
AccIII TCCGGA 1 cut(s) 1453
AclI AACGTT 1 cut(s) 2300
AclWI GGATC 5 cut(s) 700, 746, 1227, 1848, 2524
AcoI YGGCCR 2 cut(s) 2334, 2523
AcsI RAATTY 6 cut(s) 380, 542, 1064, 1121, 1178, 2452
AcuI CTGAAG 4 cut(s) 584, 794, 2133, 2259
AcyI GRCGYC 2 cut(s) 339, 1509
AdeI CACNNNGTG 1 cut(s) 1891
AfaI GTAC 6 cut(s) 473, 704, 945, 1340, 1802, 2183
AfeI AGCGCT 1 cut(s) 1830
AfiI CCNNNNNNNGG 9 cut(s) 422, 659, 686, 719, 768, 2223, 2243, 2284, 2403
AgeI ACCGGT 1 cut(s) 2511
AjiI CACGTC 1 cut(s) 677
AjnI CCWGG 6 cut(s) 30, 415, 1012, 2030, 2081, 2563
AjuI GAANNNNNNNTTGG 2 cut(s) 318, 350
Alw21I GWGCWC 3 cut(s) 1600, 2268, 2376
Alw26I GTCTC 3 cut(s) 570, 804, 1175
AlwI GGATC 5 cut(s) 700, 746, 1227, 1848, 2524
AlwNI CAGNNNCTG 2 cut(s) 1587, 1610
Ama87I CYCGRG 1 cut(s) 343
Aor13HI TCCGGA 1 cut(s) 1453
Aor51HI AGCGCT 1 cut(s) 1830
AoxI GGCC 5 cut(s) 2056, 2087, 2334, 2469, 2523
ApeKI GCWGC 5 cut(s) 4, 299, 1922, 2162, 2314
ApoI RAATTY 6 cut(s) 380, 542, 1064, 1121, 1178, 2452
ArsI GACNNNNNNTTYG 6 cut(s) 414, 446, 803, 835, 1106, 1138
AsiGI ACCGGT 1 cut(s) 2511
Asp700I GAANNNNTTC 1 cut(s) 411
AspLEI GCGC 2 cut(s) 1831, 2049
AspS9I GGNCC 4 cut(s) 662, 824, 877, 2519
AsuC2I CCSGG 2 cut(s) 344, 345
AsuHPI GGTGA 3 cut(s) 277, 1264, 1646
AsuII TTCGAA 2 cut(s) 927, 1039
AvaI CYCGRG 1 cut(s) 343
AvaII GGWCC 4 cut(s) 662, 824, 877, 2519
AxyI CCTNAGG 2 cut(s) 873, 1343
BaeGI GKGCMC 1 cut(s) 211
BaeI ACNNNNGTAYC 2 cut(s) 27, 60
BalI TGGCCA 1 cut(s) 2336
BanI GGYRCC 2 cut(s) 206, 2358
BanII GRGCYC 3 cut(s) 1600, 2268, 2376
BbsI GAAGAC 2 cut(s) 398, 739
Bbv12I GWGCWC 3 cut(s) 1600, 2268, 2376
BbvCI CCTCAGC 1 cut(s) 2310
BbvI GCAGC 4 cut(s) 286, 1909, 2174, 2301
BccI CCATC 7 cut(s) 875, 974, 1040, 1403, 1451, 1774, 2163
BceAI ACGGC 2 cut(s) 1321, 1903
BcgI CGANNNNNNTGC 2 cut(s) 1106, 1140
BciT130I CCWGG 6 cut(s) 32, 417, 1014, 2032, 2083, 2565
BciVI GTATCC 5 cut(s) 27, 28, 468, 978, 2559
BclI TGATCA 4 cut(s) 1083, 1822, 1845, 1996
BcnI CCSGG 2 cut(s) 344, 345
BcoDI GTCTC 3 cut(s) 570, 804, 1175
BfaI CTAG 5 cut(s) 560, 1191, 1199, 1500, 2556
BfmI CTRYAG 2 cut(s) 536, 2426
BfoI RGCGCY 1 cut(s) 1832
BfuAI ACCTGC 2 cut(s) 576, 1727
BfuI GTATCC 5 cut(s) 27, 28, 468, 978, 2559
BglII AGATCT 2 cut(s) 786, 898
BisI GCNGC 7 cut(s) 5, 300, 718, 1629, 1923, 2163, 2315
BlsI GCNGC 7 cut(s) 6, 301, 719, 1630, 1924, 2164, 2316
BmcAI AGTACT 2 cut(s) 945, 2183
Bme1390I CCNGG 8 cut(s) 32, 344, 345, 417, 1014, 2032, 2083, 2565
Bme18I GGWCC 4 cut(s) 662, 824, 877, 2519
BmeT110I CYCGRG 1 cut(s) 343
BmgBI CACGTC 1 cut(s) 677
BmgT120I GGNCC 4 cut(s) 662, 824, 877, 2519
BmiI GGNNCC 5 cut(s) 208, 663, 664, 826, 2360
BmrFI CCNGG 8 cut(s) 32, 344, 345, 417, 1014, 2032, 2083, 2565
BmrI ACTGGG 1 cut(s) 271
BmsI GCATC 7 cut(s) 379, 1315, 1323, 1684, 1718, 1889, 2404
BmuI ACTGGG 1 cut(s) 271
BpiI GAAGAC 2 cut(s) 398, 739
BplI GAGNNNNNCTC 2 cut(s) 888, 920
BpmI CTGGAG 2 cut(s) 1164, 2235
Bpu10I CCTNAGC 3 cut(s) 2219, 2267, 2310
Bpu14I TTCGAA 2 cut(s) 927, 1039
BpuEI CTTGAG 1 cut(s) 1062
BpuMI CCSGG 2 cut(s) 344, 345
BsaBI GATNNNNATC 1 cut(s) 1461
BsaHI GRCGYC 2 cut(s) 339, 1509
BsaJI CCNNGG 8 cut(s) 30, 343, 718, 1013, 1504, 2081, 2337, 2397
BsaWI WCCGGW 2 cut(s) 1453, 2511
BsaXI ACNNNNNCTCC 8 cut(s) 339, 369, 1155, 1185, 1848, 1878, 1961, 1991
Bsc4I CCNNNNNNNGG 9 cut(s) 422, 659, 686, 719, 768, 2223, 2243, 2284, 2403
Bse118I RCCGGY 3 cut(s) 2223, 2511, 2521
Bse1I ACTGG 7 cut(s) 266, 680, 1070, 1592, 1814, 2194, 2592
Bse21I CCTNAGG 2 cut(s) 873, 1343
Bse3DI GCAATG 1 cut(s) 1803
Bse8I GATNNNNATC 1 cut(s) 1461
BseAI TCCGGA 1 cut(s) 1453
BseBI CCWGG 6 cut(s) 32, 417, 1014, 2032, 2083, 2565
BseDI CCNNGG 8 cut(s) 30, 343, 718, 1013, 1504, 2081, 2337, 2397
BseGI GGATG 8 cut(s) 867, 1338, 1450, 1462, 1675, 1709, 2419, 2500
BseJI GATNNNNATC 1 cut(s) 1461
BseLI CCNNNNNNNGG 9 cut(s) 422, 659, 686, 719, 768, 2223, 2243, 2284, 2403
BseMI GCAATG 1 cut(s) 1803
BseMII CTCAG 8 cut(s) 879, 887, 1334, 1453, 1602, 2281, 2324, 2427
BseNI ACTGG 7 cut(s) 266, 680, 1070, 1592, 1814, 2194, 2592
BseRI GAGGAG 4 cut(s) 588, 606, 1869, 2276
BseSI GKGCMC 1 cut(s) 211
BseXI GCAGC 4 cut(s) 286, 1909, 2174, 2301
Bsh1236I CGCG 3 cut(s) 720, 2049, 2399
BshFI GGCC 5 cut(s) 2058, 2089, 2336, 2471, 2525
BshNI GGYRCC 2 cut(s) 206, 2358
BshTI ACCGGT 1 cut(s) 2511
BsiHKAI GWGCWC 3 cut(s) 1600, 2268, 2376
BsiHKCI CYCGRG 1 cut(s) 343
BsiSI CCGG 6 cut(s) 344, 1454, 1563, 2224, 2512, 2522
BslFI GGGAC 7 cut(s) 13, 326, 623, 648, 1426, 1705, 2186
BslI CCNNNNNNNGG 9 cut(s) 422, 659, 686, 719, 768, 2223, 2243, 2284, 2403
BsmAI GTCTC 3 cut(s) 570, 804, 1175
BsmBI CGTCTC 3 cut(s) 570, 804, 1175
BsmFI GGGAC 7 cut(s) 13, 326, 623, 648, 1426, 1705, 2186
BsmI GAATGC 3 cut(s) 7, 1705, 2288
BsnI GGCC 5 cut(s) 2058, 2089, 2336, 2471, 2525
BsoBI CYCGRG 1 cut(s) 343
Bsp119I TTCGAA 2 cut(s) 927, 1039
Bsp1286I GDGCHC 4 cut(s) 211, 1600, 2268, 2376
Bsp13I TCCGGA 1 cut(s) 1453
Bsp1407I TGTACA 1 cut(s) 1800
BspANI GGCC 5 cut(s) 2058, 2089, 2336, 2471, 2525
BspCNI CTCAG 8 cut(s) 880, 886, 1335, 1452, 1603, 2280, 2323, 2428
BspEI TCCGGA 1 cut(s) 1453
BspFNI CGCG 3 cut(s) 720, 2049, 2399
BspHI TCATGA 1 cut(s) 1290
BspLI GGNNCC 5 cut(s) 208, 663, 664, 826, 2360
BspMAI CTGCAG 1 cut(s) 2430
BspMI ACCTGC 2 cut(s) 576, 1727
BspPI GGATC 5 cut(s) 700, 746, 1227, 1848, 2524
BspQI GCTCTTC 1 cut(s) 549
BspT104I TTCGAA 2 cut(s) 927, 1039
BspT107I GGYRCC 2 cut(s) 206, 2358
BsrDI GCAATG 1 cut(s) 1803
BsrFI RCCGGY 3 cut(s) 2223, 2511, 2521
BsrGI TGTACA 1 cut(s) 1800
BsrI ACTGG 7 cut(s) 266, 680, 1070, 1592, 1814, 2194, 2592
BssAI RCCGGY 3 cut(s) 2223, 2511, 2521
BssECI CCNNGG 8 cut(s) 30, 343, 718, 1013, 1504, 2081, 2337, 2397
BssNAI GTATAC 1 cut(s) 799
BssNI GRCGYC 2 cut(s) 339, 1509
BssT1I CCWWGG 1 cut(s) 1504
Bst1107I GTATAC 1 cut(s) 799
Bst2UI CCWGG 6 cut(s) 32, 417, 1014, 2032, 2083, 2565
Bst4CI ACNGT 6 cut(s) 43, 377, 485, 1188, 2177, 2341
Bst6I CTCTTC 1 cut(s) 549
BstACI GRCGYC 2 cut(s) 339, 1509
BstAUI TGTACA 1 cut(s) 1800
BstBI TTCGAA 2 cut(s) 927, 1039
BstDSI CCRYGG 3 cut(s) 718, 2337, 2397
BstEII GGTNACC 1 cut(s) 265
BstENI CCTNNNNNAGG 2 cut(s) 420, 2241
BstF5I GGATG 8 cut(s) 867, 1338, 1450, 1462, 1675, 1709, 2419, 2500
BstFNI CGCG 3 cut(s) 720, 2049, 2399
BstH2I RGCGCY 1 cut(s) 1832
BstHHI GCGC 2 cut(s) 1831, 2049
BstMAI GTCTC 3 cut(s) 570, 804, 1175
BstMWI GCNNNNNNNGC 7 cut(s) 296, 1835, 2055, 2095, 2159, 2233, 2468
BstNI CCWGG 6 cut(s) 32, 417, 1014, 2032, 2083, 2565
BstPI GGTNACC 1 cut(s) 265
BstSCI CCNGG 8 cut(s) 30, 342, 343, 415, 1012, 2030, 2081, 2563
BstSFI CTRYAG 2 cut(s) 536, 2426
BstSLI GKGCMC 1 cut(s) 211
BstUI CGCG 3 cut(s) 720, 2049, 2399
BstV1I GCAGC 4 cut(s) 286, 1909, 2174, 2301
BstV2I GAAGAC 2 cut(s) 398, 739
BstX2I RGATCY 6 cut(s) 692, 751, 786, 898, 1840, 2529
BstYI RGATCY 6 cut(s) 692, 751, 786, 898, 1840, 2529
BstZ17I GTATAC 1 cut(s) 799
Bsu36I CCTNAGG 2 cut(s) 873, 1343
BsuI GTATCC 5 cut(s) 27, 28, 468, 978, 2559
BsuRI GGCC 5 cut(s) 2058, 2089, 2336, 2471, 2525
BtgI CCRYGG 3 cut(s) 718, 2337, 2397
BtrI CACGTC 1 cut(s) 677
BtsCI GGATG 8 cut(s) 867, 1338, 1450, 1462, 1675, 1709, 2419, 2500
BtsI GCAGTG 1 cut(s) 1611
BtsIMutI CAGTG 5 cut(s) 382, 1077, 1585, 1611, 2364
BveI ACCTGC 2 cut(s) 576, 1727
CaiI CAGNNNCTG 2 cut(s) 1587, 1610
CciI TCATGA 1 cut(s) 1290
CfoI GCGC 2 cut(s) 1831, 2049
Cfr10I RCCGGY 3 cut(s) 2223, 2511, 2521
Cfr13I GGNCC 4 cut(s) 662, 824, 877, 2519
Cfr42I CCGCGG 2 cut(s) 721, 2400
Cfr9I CCCGGG 1 cut(s) 343
CpoI CGGWCCG 1 cut(s) 2519
CseI GACGC 3 cut(s) 328, 1498, 1883
Csp6I GTAC 6 cut(s) 472, 703, 944, 1339, 1801, 2182
CspAI ACCGGT 1 cut(s) 2511
CspCI CAANNNNNGTGG 4 cut(s) 422, 457, 2015, 2050
CspI CGGWCCG 1 cut(s) 2519
CviQI GTAC 6 cut(s) 472, 703, 944, 1339, 1801, 2182
DraI TTTAAA 1 cut(s) 2446
DraIII CACNNNGTG 1 cut(s) 1891
EaeI YGGCCR 2 cut(s) 2334, 2523
Eam1104I CTCTTC 1 cut(s) 549
EarI CTCTTC 1 cut(s) 549
EciI GGCGGA 1 cut(s) 1363
Ecl136II GAGCTC 3 cut(s) 1598, 2266, 2374
Eco130I CCWWGG 1 cut(s) 1504
Eco24I GRGCYC 3 cut(s) 1600, 2268, 2376
Eco47I GGWCC 4 cut(s) 662, 824, 877, 2519
Eco47III AGCGCT 1 cut(s) 1830
Eco53kI GAGCTC 3 cut(s) 1598, 2266, 2374
Eco57I CTGAAG 4 cut(s) 584, 794, 2133, 2259
Eco81I CCTNAGG 2 cut(s) 873, 1343
Eco88I CYCGRG 1 cut(s) 343
Eco91I GGTNACC 1 cut(s) 265
EcoICRI GAGCTC 3 cut(s) 1598, 2266, 2374
EcoNI CCTNNNNNAGG 2 cut(s) 420, 2241
EcoO109I RGGNCCY 2 cut(s) 662, 877
EcoO65I GGTNACC 1 cut(s) 265
EcoRI GAATTC 1 cut(s) 380
EcoRII CCWGG 6 cut(s) 30, 415, 1012, 2030, 2081, 2563
EcoT14I CCWWGG 1 cut(s) 1504
EcoT22I ATGCAT 2 cut(s) 631, 1330
EcoT38I GRGCYC 3 cut(s) 1600, 2268, 2376
ErhI CCWWGG 1 cut(s) 1504
Esp3I CGTCTC 3 cut(s) 570, 804, 1175
FalI AAGNNNNNCTT 4 cut(s) 930, 962, 2276, 2308
FaqI GGGAC 7 cut(s) 13, 326, 623, 648, 1426, 1705, 2186
FauI CCCGC 1 cut(s) 2042
FauNDI CATATG 1 cut(s) 1918
FbaI TGATCA 4 cut(s) 1083, 1822, 1845, 1996
FblI GTMKAC 2 cut(s) 798, 1897
Fnu4HI GCNGC 7 cut(s) 5, 300, 718, 1629, 1923, 2163, 2315
FokI GGATG 8 cut(s) 854, 1345, 1437, 1469, 1662, 1696, 2426, 2507
FriOI GRGCYC 3 cut(s) 1600, 2268, 2376
Fsp4HI GCNGC 7 cut(s) 5, 300, 718, 1629, 1923, 2163, 2315
FspBI CTAG 5 cut(s) 560, 1191, 1199, 1500, 2556
GlaI GCGC 2 cut(s) 1830, 2048
GluI GCNGC 7 cut(s) 5, 300, 718, 1629, 1923, 2163, 2315
GsuI CTGGAG 2 cut(s) 1164, 2235
HaeII RGCGCY 1 cut(s) 1832
HaeIII GGCC 5 cut(s) 2058, 2089, 2336, 2471, 2525
HapII CCGG 6 cut(s) 344, 1454, 1563, 2224, 2512, 2522
HgaI GACGC 3 cut(s) 328, 1498, 1883
HhaI GCGC 2 cut(s) 1831, 2049
Hin1I GRCGYC 2 cut(s) 339, 1509
Hin6I GCGC 2 cut(s) 1829, 2047
HinP1I GCGC 2 cut(s) 1829, 2047
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HindIII AAGCTT 3 cut(s) 132, 776, 1371
HpaII CCGG 6 cut(s) 344, 1454, 1563, 2224, 2512, 2522
HphI GGTGA 3 cut(s) 277, 1264, 1646
Hpy166II GTNNAC 7 cut(s) 121, 154, 433, 565, 799, 1898, 2406
Hpy188I TCNGA 6 cut(s) 841, 1845, 2020, 2384, 2437, 2519
Hpy8I GTNNAC 7 cut(s) 121, 154, 433, 565, 799, 1898, 2406
Hpy99I CGWCG 2 cut(s) 1170, 1514
HpyAV CCTTC 7 cut(s) 97, 416, 953, 1990, 2032, 2237, 2562
HpyCH4III ACNGT 6 cut(s) 43, 377, 485, 1188, 2177, 2341
HpyCH4IV ACGT 4 cut(s) 676, 801, 1165, 2300
HpyF10VI GCNNNNNNNGC 7 cut(s) 296, 1835, 2055, 2095, 2159, 2233, 2468
HpySE526I ACGT 4 cut(s) 676, 801, 1165, 2300
Hsp92I GRCGYC 2 cut(s) 339, 1509
HspAI GCGC 2 cut(s) 1829, 2047
KflI GGGWCCC 1 cut(s) 662
Kpn2I TCCGGA 1 cut(s) 1453
KroI GCCGGC 1 cut(s) 2223
KroNI GCCGGC 1 cut(s) 2225
Ksp22I TGATCA 4 cut(s) 1083, 1822, 1845, 1996
KspI CCGCGG 2 cut(s) 721, 2400
LguI GCTCTTC 1 cut(s) 549
LmnI GCTCC 5 cut(s) 347, 1030, 1836, 1982, 2263
Lsp1109I GCAGC 4 cut(s) 286, 1909, 2174, 2301
LweI GCATC 7 cut(s) 379, 1315, 1323, 1684, 1718, 1889, 2404
MaeI CTAG 5 cut(s) 560, 1191, 1199, 1500, 2556
MaeII ACGT 4 cut(s) 676, 801, 1165, 2300
MaeIII GTNAC 6 cut(s) 265, 310, 911, 1156, 1606, 2390
MflI RGATCY 6 cut(s) 692, 751, 786, 898, 1840, 2529
MhlI GDGCHC 4 cut(s) 211, 1600, 2268, 2376
MlsI TGGCCA 1 cut(s) 2336
MluNI TGGCCA 1 cut(s) 2336
MlyI GAGTC 4 cut(s) 388, 438, 1263, 1553
MmeI TCCRAC 1 cut(s) 217
Mox20I TGGCCA 1 cut(s) 2336
Mph1103I ATGCAT 2 cut(s) 631, 1330
MroI TCCGGA 1 cut(s) 1453
MroNI GCCGGC 1 cut(s) 2223
MroXI GAANNNNTTC 1 cut(s) 411
MscI TGGCCA 1 cut(s) 2336
MslI CAYNNNNRTG 6 cut(s) 60, 375, 833, 1323, 1455, 1686
Msp20I TGGCCA 1 cut(s) 2336
MspA1I CMGCKG 3 cut(s) 720, 2314, 2399
MspI CCGG 6 cut(s) 344, 1454, 1563, 2224, 2512, 2522
MspR9I CCNGG 8 cut(s) 32, 344, 345, 417, 1014, 2032, 2083, 2565
Mva1269I GAATGC 3 cut(s) 7, 1705, 2288
MvaI CCWGG 6 cut(s) 32, 417, 1014, 2032, 2083, 2565
MvnI CGCG 3 cut(s) 720, 2049, 2399
MwoI GCNNNNNNNGC 7 cut(s) 296, 1835, 2055, 2095, 2159, 2233, 2468
NaeI GCCGGC 1 cut(s) 2225
NciI CCSGG 2 cut(s) 344, 345
NdeI CATATG 1 cut(s) 1918
NgoMIV GCCGGC 1 cut(s) 2223
NlaIV GGNNCC 5 cut(s) 208, 663, 664, 826, 2360
NmuCI GTSAC 4 cut(s) 265, 1156, 1606, 2390
NsiI ATGCAT 2 cut(s) 631, 1330
NspV TTCGAA 2 cut(s) 927, 1039
PagI TCATGA 1 cut(s) 1290
PaqCI CACCTGC 1 cut(s) 1727
PciSI GCTCTTC 1 cut(s) 549
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PctI GAATGC 3 cut(s) 7, 1705, 2288
PdiI GCCGGC 1 cut(s) 2225
PdmI GAANNNNTTC 1 cut(s) 411
PfeI GAWTC 7 cut(s) 139, 649, 1660, 1871, 2015, 2120, 2196
PflFI GACNNNGTC 1 cut(s) 614
PflMI CCANNNNNTGG 1 cut(s) 686
PinAI ACCGGT 1 cut(s) 2511
PkrI GCNGC 7 cut(s) 6, 301, 719, 1630, 1924, 2164, 2316
PleI GAGTC 4 cut(s) 388, 437, 1263, 1553
PpsI GAGTC 4 cut(s) 388, 437, 1263, 1553
PpuMI RGGWCCY 2 cut(s) 662, 877
Psp124BI GAGCTC 3 cut(s) 1600, 2268, 2376
Psp1406I AACGTT 1 cut(s) 2300
Psp5II RGGWCCY 2 cut(s) 662, 877
Psp6I CCWGG 6 cut(s) 30, 415, 1012, 2030, 2081, 2563
PspEI GGTNACC 1 cut(s) 265
PspGI CCWGG 6 cut(s) 30, 415, 1012, 2030, 2081, 2563
PspN4I GGNNCC 5 cut(s) 208, 663, 664, 826, 2360
PspPI GGNCC 4 cut(s) 662, 824, 877, 2519
PspPPI RGGWCCY 2 cut(s) 662, 877
PstI CTGCAG 1 cut(s) 2430
PstNI CAGNNNCTG 2 cut(s) 1587, 1610
PsuI RGATCY 6 cut(s) 692, 751, 786, 898, 1840, 2529
PsyI GACNNNGTC 1 cut(s) 614
PvuII CAGCTG 1 cut(s) 2314
RsaI GTAC 6 cut(s) 473, 704, 945, 1340, 1802, 2183
RsaNI GTAC 6 cut(s) 472, 703, 944, 1339, 1801, 2182
RseI CAYNNNNRTG 6 cut(s) 60, 375, 833, 1323, 1455, 1686
Rsr2I CGGWCCG 1 cut(s) 2519
RsrII CGGWCCG 1 cut(s) 2519
SacI GAGCTC 3 cut(s) 1600, 2268, 2376
SacII CCGCGG 2 cut(s) 721, 2400
SapI GCTCTTC 1 cut(s) 549
SatI GCNGC 7 cut(s) 5, 300, 718, 1629, 1923, 2163, 2315
Sau96I GGNCC 4 cut(s) 662, 824, 877, 2519
ScaI AGTACT 2 cut(s) 945, 2183
SchI GAGTC 4 cut(s) 388, 438, 1263, 1553
ScrFI CCNGG 8 cut(s) 32, 344, 345, 417, 1014, 2032, 2083, 2565
SduI GDGCHC 4 cut(s) 211, 1600, 2268, 2376
SfaNI GCATC 7 cut(s) 379, 1315, 1323, 1684, 1718, 1889, 2404
SfcI CTRYAG 2 cut(s) 536, 2426
Sfr303I CCGCGG 2 cut(s) 721, 2400
SfuI TTCGAA 2 cut(s) 927, 1039
SgrBI CCGCGG 2 cut(s) 721, 2400
SinI GGWCC 4 cut(s) 662, 824, 877, 2519
SmaI CCCGGG 1 cut(s) 345
SmiMI CAYNNNNRTG 6 cut(s) 60, 375, 833, 1323, 1455, 1686
SmlI CTYRAG 1 cut(s) 1077
SmoI CTYRAG 1 cut(s) 1077
SspI AATATT 1 cut(s) 1761
SspMI CTAG 5 cut(s) 560, 1191, 1199, 1500, 2556
SstI GAGCTC 3 cut(s) 1600, 2268, 2376
StyD4I CCNGG 8 cut(s) 30, 342, 343, 415, 1012, 2030, 2081, 2563
StyI CCWWGG 1 cut(s) 1504
TaaI ACNGT 6 cut(s) 43, 377, 485, 1188, 2177, 2341
TaiI ACGT 4 cut(s) 679, 804, 1168, 2303
TaqI TCGA 9 cut(s) 114, 137, 405, 427, 927, 1039, 1512, 1778, 2186
TaqII GACCGA 1 cut(s) 1218
TatI WGTACW 4 cut(s) 471, 943, 1800, 2181
TauI GCSGC 2 cut(s) 720, 1631
TfiI GAWTC 7 cut(s) 139, 649, 1660, 1871, 2015, 2120, 2196
TscAI CASTG 5 cut(s) 382, 1077, 1592, 1611, 2371
TseFI GTSAC 4 cut(s) 265, 1156, 1606, 2390
TseI GCWGC 5 cut(s) 4, 299, 1922, 2162, 2314
Tsp45I GTSAC 4 cut(s) 265, 1156, 1606, 2390
TspDTI ATGAA 8 cut(s) 113, 597, 972, 1297, 1307, 1712, 2221, 2501
TspGWI ACGGA 2 cut(s) 1485, 2426
TspMI CCCGGG 1 cut(s) 343
TspRI CASTG 5 cut(s) 382, 1077, 1592, 1611, 2371
Tth111I GACNNNGTC 1 cut(s) 614
Van91I CCANNNNNTGG 1 cut(s) 686
VpaK11BI GGWCC 4 cut(s) 662, 824, 877, 2519
XagI CCTNNNNNAGG 2 cut(s) 420, 2241
XapI RAATTY 6 cut(s) 380, 542, 1064, 1121, 1178, 2452
XcmI CCANNNNNNNNNTGG 1 cut(s) 1777
XmaI CCCGGG 1 cut(s) 343
XmiI GTMKAC 2 cut(s) 798, 1897
XmnI GAANNNNTTC 1 cut(s) 411
XspI CTAG 5 cut(s) 560, 1191, 1199, 1500, 2556
ZrmI AGTACT 2 cut(s) 945, 2183
Zsp2I ATGCAT 2 cut(s) 631, 1330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.