MD12G1218000.v1.1

Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000763
Physical Location & Seq
Reverse (-)
29558554 .. 29562100
3547 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1218000.v1.1.491

Sequence Viewer

Length: 2769 bp
ATGCTGCATTCCAAGTATCTCACAGCTCCTTTGAGCTTTCGGAGACAATGTCCGTCTCGGAATCGGATGAAGAACTCAGTTGTTGCAGGCAACAACCTTTCATCACTCAATCACATGAGACCAATACTTTTGCCTATGATTACACGATCTCAACCGTTGAAGCTATCTTCAACGGTTGTTAAGGCATCATCCCAGGACACCACCGTTGCTACGCAGCCGAAACATGATGGCAAGAAGATCAAAGGGAGGGTGGTGTTGATGAAGAAAATTGTTCTCGACTTGAACGACCTTAAAGCTTCGATTCTTGATCGTGTTCACGAGCTGTTAGGCAAAGCGGTTTCTCTAAGGCTCATTAGTTCGGTTAATAGTGACCCTGAAAATGAGTCGACAGGAAAAGTTGGAAAGCCGGCATACTTGGAGAATTGGGTGACTACAATCACCCCCTTAACAGCAGGGGAGACTGCATTTGATGTTACTTTCGATTGGGACGAGGAAATTGGCGTCCCGGGAGCATTTATAGTTAGAAATTATCATCACAGTGAGTTCTACTTGAAGACTGTCACCCTCGAAGATGTTCCTGGCGAAGGTCGAGTCCACTTTGTTTGCAACTCATGGGTATATCCTGCTGACAAGTACAAAAAAGACCGTGTTTTCTTCGCTAACAAGACTTATCTTCCAGGTGATACACCAAAATCACTACAGAAATTCAGAGAAGAAGAGCTAGTAAACCTGCGAGGAGACGATGAAGAACGAGGAGAGCTTCAGGAATGGGACAGGGTCTATGACTATGCATACTACAATGATTTGGGGAATCCAGATAAGGGTCCCGAATATGCGCGTCCAGTTCTCGGAGGGTCTATTGAGTACCCATATCCTCGCAGGGGAAGAACAGGAAGACCAGCAACAAAAACGGATCCTAACACCGAGAGCAGGCTGAAGCTTATCCAGAGATTAAATGTATATGTTCCGAGAGATGAACGATTTGGACCCTTAAAGATGTCAGATTTGCTTGCTTATGTCCTGAAATCCATCCCTCAGGTCCTTAAACCTGAGATAAAAGCTCTACTCCTTGGTAACAAAAACGAGTTCGAAACCATGGAAGACGTACTTAAACTCTATGAAGGAGGATTAGAGTTGCCTGATGGGATACTAAAACACATTAGTGATAGCATTCCGGAGGAGATGTTCAAAGAACTTTTTCGAACTGACGGCGAAAAGTTCCTAAAATTTCCAGTGCCTCAAGTGATCAAAGAGGATAAGTCGGCATGGAGAACTGACGAAGAATTTGCAAGAGAACTGCTGGCTGGAGTAAATCCTGTTACAATACGTCGTCTCCAAGAATTTCCACCGGCTAGCAAGCTAGACCGAGAAGCATACGGGGATCAAACAAGTCAAATAACCAAAGAACATATAGAACATAACTTAAATGGACTCAGCATAGATGAGGCAATCAAGAACAAGCAGCTATTCATATTAGACCACCACGATGCATGGATGCCGTACCTGAGGCGGATAAACACAACTTCCACCAAAGCATACGCAAGCAGAACACTCCTTTTCTTGAACAATGATGGGACTTTGAAGCCAATAGCTATTGAACTCAGCTTGCCACATCCGGATGGAGATCAGTTTGGCTGCATTAGCAAAGTCTATACTCCGTCTAGCCAAGGCGTTGAGAGTTCCATTTGGCAACTTGCTAAAGCTTATGCACTAGTAAATGACTCCGGCTGTCATCAGCTTTTCAGCCACTGGTTGAGAGCTCATGCAGTGACTGAGCCATTTGTGATAGCGGCAAATAGGCAGTTGAGCGTGCTTCACCCGATTCATAAACTTCTGCATCCTCATTTTCGTGAGACTATGAATGCAAATGCAGCTGCGCGACAGGTTCTCACAAACGCTGGTGGAGTAATAGAGGAAATAATCTTTGCTTCAAAGTTTTCAATGGAATGGTCATCAGCAATGTACAAGGACTGGACTTTTCCTGATCAAGCACTCCCCATGGATCTGATCAAAAGAGGAATGGCAGTTGAAGATTCAAGCGCGTCACACGGTGTGCGTTTACTGATAGAGGACTATCCATATGCTGCAGACGGGCTTGAATTATGGTCTGCAATTAAAACATGGGTTAAGGACTATTGCTCCTTCTATTACAAAAATGATCAAACGGTTCAAAATGATTCGGAACTCCAATCCTGGTGGAAGGAACTCCGCGAGGTAGGCCACGGAGACAAGAAAGATGAACCCTGGTGGCCTAAAATGCAGACTCGTGACGAGCTGATAGAATCATGCACAACTATCATATATATTGCTTCAGCCCATCATGCAGCAATCAACTACGGTCAGTACTCGATTGGTGGATTCGTCCCAAACCGCCCAACCATAAGCCGGCGGTTCATGCCTGAAGAAGGCACTCCAGAGTATGAGGAGCTGAGGGCAAACCCTGACAAGGCATTCTTGAAAACGTTTGCACCTCAGCTGCCGACACTTCTTGGAATGGCCACGGTGGAAATCTTGTCAAGGCACCCCGCTGATGAGCTCTATCTCGGACAGCGTGACACCGCGGAGTGGACAACGGATGCAGACATACTGCAGGCTTCTGAGGATTTTAAAAAGAAGTTGGAAGCGATTGAGGCCAAAATTGAAAAGATGAACAAGGATGAGAGATTGAAAAACCGGTTCGGACCGGCGAAGATCCCTTACACTTTGCTGTATCCTTCTAGTGAACCTGGACTTACTAACAAGGGAATTCCCACCAGTATCAACATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00591 map00591
Pfam Domains
Protein Families
Lipoxygenase PLAT

Protein Analysis

923

Amino Acids

104.89

Weight (kDa)

6.25

Isoelectric Point (pI)

45.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAT PF01477 116 - 219 7.4e-24 PLAT/LH2 domain
Lipoxygenase PF00305 233 - 905 0.0 Lipoxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 738
AccB1I GGYRCC 1 cut(s) 2520
AccI GTMKAC 1 cut(s) 386
AccII CGCG 5 cut(s) 838, 1879, 2042, 2211, 2561
AccIII TCCGGA 2 cut(s) 1174, 1615
AciI CCGC 9 cut(s) 335, 1510, 1790, 2209, 2371, 2389, 2526, 2559, 2561
AclI AACGTT 1 cut(s) 2462
AclWI GGATC 5 cut(s) 908, 921, 1389, 2010, 2686
AcoI YGGCCR 1 cut(s) 2496
AcsI RAATTY 5 cut(s) 704, 1226, 1283, 1340, 2745
AcuI CTGAAG 4 cut(s) 746, 956, 2295, 2421
AcyI GRCGYC 1 cut(s) 501
AdeI CACNNNGTG 2 cut(s) 2051, 2053
AfaI GTAC 6 cut(s) 635, 866, 1107, 1502, 1964, 2345
AfiI CCNNNNNNNGG 9 cut(s) 584, 821, 848, 881, 930, 2385, 2405, 2446, 2565
AgeI ACCGGT 1 cut(s) 2673
AhlI ACTAGT 1 cut(s) 1711
AjnI CCWGG 6 cut(s) 192, 577, 676, 2192, 2243, 2725
AleI CACNNNNGTG 1 cut(s) 1161
AloI GAACNNNNNNTCC 2 cut(s) 1169, 1201
Alw21I GWGCWC 2 cut(s) 1762, 2538
Alw26I GTCTC 8 cut(s) 37, 60, 112, 452, 732, 1337, 1847, 2220
AlwI GGATC 5 cut(s) 908, 921, 1389, 2010, 2686
AlwNI CAGNNNCTG 2 cut(s) 1749, 1772
Ama87I CYCGRG 1 cut(s) 505
Aor13HI TCCGGA 2 cut(s) 1174, 1615
AoxI GGCC 4 cut(s) 2218, 2249, 2496, 2631
ApeKI GCWGC 9 cut(s) 4, 214, 1462, 1635, 1871, 1874, 2084, 2324, 2476
ApoI RAATTY 5 cut(s) 704, 1226, 1283, 1340, 2745
ArsI GACNNNNNNTTYG 4 cut(s) 576, 608, 1268, 1300
AsiGI ACCGGT 1 cut(s) 2673
Asp700I GAANNNNTTC 2 cut(s) 573, 1197
AspLEI GCGC 3 cut(s) 838, 1879, 2042
AspS9I GGNCC 4 cut(s) 824, 986, 1039, 2681
AsuC2I CCSGG 2 cut(s) 506, 507
AsuHPI GGTGA 5 cut(s) 430, 439, 553, 692, 1808
AsuII TTCGAA 2 cut(s) 1089, 1201
AsuNHI GCTAGC 1 cut(s) 1352
AvaI CYCGRG 1 cut(s) 505
AvaII GGWCC 4 cut(s) 824, 986, 1039, 2681
AxyI CCTNAGG 2 cut(s) 1035, 1505
BalI TGGCCA 1 cut(s) 2498
BamHI GGATCC 1 cut(s) 913
BanI GGYRCC 1 cut(s) 2520
BanII GRGCYC 2 cut(s) 1762, 2538
BarI GAAGNNNNNNTAC 2 cut(s) 2681, 2713
BauI CACGAG 2 cut(s) 317, 2265
BbsI GAAGAC 3 cut(s) 560, 901, 1107
Bbv12I GWGCWC 2 cut(s) 1762, 2538
BbvCI CCTCAGC 2 cut(s) 2429, 2472
BbvI GCAGC 8 cut(s) 226, 1474, 1622, 1861, 1883, 2071, 2336, 2463
BccI CCATC 6 cut(s) 221, 1037, 1136, 1565, 1613, 2325
BceAI ACGGC 2 cut(s) 1225, 1483
BcgI CGANNNNNNTGC 2 cut(s) 1268, 1302
BciT130I CCWGG 6 cut(s) 194, 579, 678, 2194, 2245, 2727
BciVI GTATCC 2 cut(s) 1140, 2721
BclI TGATCA 4 cut(s) 1245, 1984, 2007, 2158
BcnI CCSGG 2 cut(s) 506, 507
BcoDI GTCTC 8 cut(s) 37, 60, 112, 452, 732, 1337, 1847, 2220
BcuI ACTAGT 1 cut(s) 1711
BfaI CTAG 6 cut(s) 722, 1353, 1361, 1662, 1712, 2718
BfmI CTRYAG 3 cut(s) 698, 2085, 2588
BfuAI ACCTGC 1 cut(s) 738
BfuI GTATCC 2 cut(s) 1140, 2721
BmcAI AGTACT 1 cut(s) 2345
Bme1390I CCNGG 8 cut(s) 194, 506, 507, 579, 678, 2194, 2245, 2727
Bme18I GGWCC 4 cut(s) 824, 986, 1039, 2681
BmeT110I CYCGRG 1 cut(s) 505
BmgT120I GGNCC 4 cut(s) 824, 986, 1039, 2681
BmiI GGNNCC 5 cut(s) 825, 826, 915, 988, 2522
BmrFI CCNGG 8 cut(s) 194, 506, 507, 579, 678, 2194, 2245, 2727
BmsI GCATC 5 cut(s) 194, 1477, 1485, 1846, 2566
BmtI GCTAGC 1 cut(s) 1356
BpiI GAAGAC 3 cut(s) 560, 901, 1107
BpmI CTGGAG 2 cut(s) 1326, 2397
Bpu10I CCTNAGC 2 cut(s) 2429, 2472
Bpu14I TTCGAA 2 cut(s) 1089, 1201
BpuEI CTTGAG 1 cut(s) 1224
BpuMI CCSGG 2 cut(s) 506, 507
BsaBI GATNNNNATC 1 cut(s) 1623
BsaHI GRCGYC 1 cut(s) 501
BsaI GGTCTC 1 cut(s) 112
BsaWI WCCGGW 3 cut(s) 1174, 1615, 2673
BsaXI ACNNNNNCTCC 8 cut(s) 34, 64, 1169, 1199, 1317, 1347, 2123, 2153
Bsc4I CCNNNNNNNGG 9 cut(s) 584, 821, 848, 881, 930, 2385, 2405, 2446, 2565
Bse118I RCCGGY 5 cut(s) 406, 1348, 2385, 2673, 2683
Bse1I ACTGG 5 cut(s) 842, 1232, 1754, 1976, 2754
Bse21I CCTNAGG 2 cut(s) 1035, 1505
Bse3DI GCAATG 1 cut(s) 1965
Bse8I GATNNNNATC 1 cut(s) 1623
BseAI TCCGGA 2 cut(s) 1174, 1615
BseBI CCWGG 6 cut(s) 194, 579, 678, 2194, 2245, 2727
BseGI GGATG 9 cut(s) 72, 188, 1029, 1500, 1612, 1624, 1837, 2581, 2662
BseJI GATNNNNATC 1 cut(s) 1623
BseLI CCNNNNNNNGG 9 cut(s) 584, 821, 848, 881, 930, 2385, 2405, 2446, 2565
BseMI GCAATG 1 cut(s) 1965
BseNI ACTGG 5 cut(s) 842, 1232, 1754, 1976, 2754
BseRI GAGGAG 4 cut(s) 750, 768, 1193, 2438
BseXI GCAGC 8 cut(s) 226, 1474, 1622, 1861, 1883, 2071, 2336, 2463
Bsh1236I CGCG 5 cut(s) 838, 1879, 2042, 2211, 2561
BshFI GGCC 4 cut(s) 2220, 2251, 2498, 2633
BshNI GGYRCC 1 cut(s) 2520
BshTI ACCGGT 1 cut(s) 2673
BsiHKAI GWGCWC 2 cut(s) 1762, 2538
BsiHKCI CYCGRG 1 cut(s) 505
BsiSI CCGG 9 cut(s) 407, 506, 1175, 1349, 1616, 1725, 2386, 2674, 2684
BslFI GGGAC 6 cut(s) 488, 500, 785, 810, 1588, 2348
BslI CCNNNNNNNGG 9 cut(s) 584, 821, 848, 881, 930, 2385, 2405, 2446, 2565
BsmAI GTCTC 8 cut(s) 37, 60, 112, 452, 732, 1337, 1847, 2220
BsmBI CGTCTC 3 cut(s) 60, 732, 1337
BsmFI GGGAC 6 cut(s) 488, 500, 785, 810, 1588, 2348
BsmI GAATGC 4 cut(s) 7, 1170, 1867, 2450
BsnI GGCC 4 cut(s) 2220, 2251, 2498, 2633
Bso31I GGTCTC 1 cut(s) 112
BsoBI CYCGRG 1 cut(s) 505
Bsp119I TTCGAA 2 cut(s) 1089, 1201
Bsp1286I GDGCHC 2 cut(s) 1762, 2538
Bsp13I TCCGGA 2 cut(s) 1174, 1615
Bsp1407I TGTACA 1 cut(s) 1962
Bsp19I CCATGG 2 cut(s) 1095, 1998
BspACI CCGC 9 cut(s) 335, 1510, 1790, 2209, 2371, 2389, 2526, 2559, 2561
BspANI GGCC 4 cut(s) 2220, 2251, 2498, 2633
BspEI TCCGGA 2 cut(s) 1174, 1615
BspFNI CGCG 5 cut(s) 838, 1879, 2042, 2211, 2561
BspLI GGNNCC 5 cut(s) 825, 826, 915, 988, 2522
BspMAI CTGCAG 2 cut(s) 2089, 2592
BspMI ACCTGC 1 cut(s) 738
BspOI GCTAGC 1 cut(s) 1356
BspPI GGATC 5 cut(s) 908, 921, 1389, 2010, 2686
BspQI GCTCTTC 1 cut(s) 711
BspT104I TTCGAA 2 cut(s) 1089, 1201
BspT107I GGYRCC 1 cut(s) 2520
BspTNI GGTCTC 1 cut(s) 112
BsrDI GCAATG 1 cut(s) 1965
BsrFI RCCGGY 5 cut(s) 406, 1348, 2385, 2673, 2683
BsrGI TGTACA 1 cut(s) 1962
BsrI ACTGG 5 cut(s) 842, 1232, 1754, 1976, 2754
BssAI RCCGGY 5 cut(s) 406, 1348, 2385, 2673, 2683
BssNI GRCGYC 1 cut(s) 501
BssSI CACGAG 2 cut(s) 317, 2265
BssT1I CCWWGG 4 cut(s) 1069, 1095, 1666, 1998
Bst2BI CACGAG 2 cut(s) 317, 2265
Bst2UI CCWGG 6 cut(s) 194, 579, 678, 2194, 2245, 2727
Bst6I CTCTTC 1 cut(s) 711
BstACI GRCGYC 1 cut(s) 501
BstAUI TGTACA 1 cut(s) 1962
BstBI TTCGAA 2 cut(s) 1089, 1201
BstDSI CCRYGG 5 cut(s) 1095, 1998, 2221, 2499, 2559
BstENI CCTNNNNNAGG 2 cut(s) 582, 2403
BstF5I GGATG 9 cut(s) 72, 188, 1029, 1500, 1612, 1624, 1837, 2581, 2662
BstFNI CGCG 5 cut(s) 838, 1879, 2042, 2211, 2561
BstHHI GCGC 3 cut(s) 838, 1879, 2042
BstMAI GTCTC 8 cut(s) 37, 60, 112, 452, 732, 1337, 1847, 2220
BstMWI GCNNNNNNNGC 7 cut(s) 1641, 1871, 2217, 2257, 2321, 2395, 2630
BstNI CCWGG 6 cut(s) 194, 579, 678, 2194, 2245, 2727
BstSCI CCNGG 8 cut(s) 192, 504, 505, 577, 676, 2192, 2243, 2725
BstSFI CTRYAG 3 cut(s) 698, 2085, 2588
BstUI CGCG 5 cut(s) 838, 1879, 2042, 2211, 2561
BstV1I GCAGC 8 cut(s) 226, 1474, 1622, 1861, 1883, 2071, 2336, 2463
BstV2I GAAGAC 3 cut(s) 560, 901, 1107
BstX2I RGATCY 3 cut(s) 913, 2002, 2691
BstYI RGATCY 3 cut(s) 913, 2002, 2691
Bsu36I CCTNAGG 2 cut(s) 1035, 1505
BsuI GTATCC 2 cut(s) 1140, 2721
BsuRI GGCC 4 cut(s) 2220, 2251, 2498, 2633
BtgI CCRYGG 5 cut(s) 1095, 1998, 2221, 2499, 2559
BtsCI GGATG 9 cut(s) 72, 188, 1029, 1500, 1612, 1624, 1837, 2581, 2662
BtsI GCAGTG 1 cut(s) 1773
BtsIMutI CAGTG 4 cut(s) 544, 1239, 1747, 1773
BveI ACCTGC 1 cut(s) 738
CaiI CAGNNNCTG 2 cut(s) 1749, 1772
CfoI GCGC 3 cut(s) 838, 1879, 2042
Cfr10I RCCGGY 5 cut(s) 406, 1348, 2385, 2673, 2683
Cfr13I GGNCC 4 cut(s) 824, 986, 1039, 2681
Cfr42I CCGCGG 1 cut(s) 2562
Cfr9I CCCGGG 1 cut(s) 505
CpoI CGGWCCG 1 cut(s) 2681
CseI GACGC 3 cut(s) 490, 827, 2031
Csp6I GTAC 6 cut(s) 634, 865, 1106, 1501, 1963, 2344
CspAI ACCGGT 1 cut(s) 2673
CspCI CAANNNNNGTGG 4 cut(s) 584, 619, 2177, 2212
CspI CGGWCCG 1 cut(s) 2681
CviQI GTAC 6 cut(s) 634, 865, 1106, 1501, 1963, 2344
DraI TTTAAA 1 cut(s) 2608
DraIII CACNNNGTG 2 cut(s) 2051, 2053
EaeI YGGCCR 1 cut(s) 2496
Eam1104I CTCTTC 1 cut(s) 711
EarI CTCTTC 1 cut(s) 711
EciI GGCGGA 1 cut(s) 1525
Ecl136II GAGCTC 2 cut(s) 1760, 2536
Eco130I CCWWGG 4 cut(s) 1069, 1095, 1666, 1998
Eco24I GRGCYC 2 cut(s) 1762, 2538
Eco31I GGTCTC 1 cut(s) 112
Eco47I GGWCC 4 cut(s) 824, 986, 1039, 2681
Eco53kI GAGCTC 2 cut(s) 1760, 2536
Eco57I CTGAAG 4 cut(s) 746, 956, 2295, 2421
Eco81I CCTNAGG 2 cut(s) 1035, 1505
Eco88I CYCGRG 1 cut(s) 505
EcoICRI GAGCTC 2 cut(s) 1760, 2536
EcoNI CCTNNNNNAGG 2 cut(s) 582, 2403
EcoO109I RGGNCCY 2 cut(s) 824, 1039
EcoRI GAATTC 1 cut(s) 2745
EcoRII CCWGG 6 cut(s) 192, 577, 676, 2192, 2243, 2725
EcoT14I CCWWGG 4 cut(s) 1069, 1095, 1666, 1998
EcoT22I ATGCAT 2 cut(s) 793, 1492
EcoT38I GRGCYC 2 cut(s) 1762, 2538
ErhI CCWWGG 4 cut(s) 1069, 1095, 1666, 1998
Esp3I CGTCTC 3 cut(s) 60, 732, 1337
FalI AAGNNNNNCTT 4 cut(s) 1092, 1124, 2438, 2470
FaqI GGGAC 6 cut(s) 488, 500, 785, 810, 1588, 2348
FauI CCCGC 1 cut(s) 2533
FauNDI CATATG 1 cut(s) 2080
FbaI TGATCA 4 cut(s) 1245, 1984, 2007, 2158
FblI GTMKAC 1 cut(s) 386
FokI GGATG 9 cut(s) 79, 175, 1016, 1507, 1599, 1631, 1824, 2588, 2669
FriOI GRGCYC 2 cut(s) 1762, 2538
FspBI CTAG 6 cut(s) 722, 1353, 1361, 1662, 1712, 2718
GlaI GCGC 3 cut(s) 837, 1878, 2041
GsuI CTGGAG 2 cut(s) 1326, 2397
HaeIII GGCC 4 cut(s) 2220, 2251, 2498, 2633
HapII CCGG 9 cut(s) 407, 506, 1175, 1349, 1616, 1725, 2386, 2674, 2684
HgaI GACGC 3 cut(s) 490, 827, 2031
HhaI GCGC 3 cut(s) 838, 1879, 2042
Hin1I GRCGYC 1 cut(s) 501
Hin6I GCGC 3 cut(s) 836, 1877, 2040
HinP1I GCGC 3 cut(s) 836, 1877, 2040
HincII GTYRAC 1 cut(s) 387
HindII GTYRAC 1 cut(s) 387
HindIII AAGCTT 3 cut(s) 294, 938, 1701
HpaII CCGG 9 cut(s) 407, 506, 1175, 1349, 1616, 1725, 2386, 2674, 2684
HphI GGTGA 5 cut(s) 430, 439, 553, 692, 1808
Hpy166II GTNNAC 7 cut(s) 316, 387, 595, 727, 2060, 2568, 2723
Hpy8I GTNNAC 7 cut(s) 316, 387, 595, 727, 2060, 2568, 2723
Hpy99I CGWCG 1 cut(s) 1332
HpyAV CCTTC 6 cut(s) 578, 1115, 2152, 2194, 2399, 2724
HpyCH4IV ACGT 3 cut(s) 1104, 1327, 2462
HpyF10VI GCNNNNNNNGC 7 cut(s) 1641, 1871, 2217, 2257, 2321, 2395, 2630
HpySE526I ACGT 3 cut(s) 1104, 1327, 2462
Hsp92I GRCGYC 1 cut(s) 501
HspAI GCGC 3 cut(s) 836, 1877, 2040
KflI GGGWCCC 1 cut(s) 824
Kpn2I TCCGGA 2 cut(s) 1174, 1615
KroI GCCGGC 2 cut(s) 406, 2385
KroNI GCCGGC 2 cut(s) 408, 2387
Ksp22I TGATCA 4 cut(s) 1245, 1984, 2007, 2158
KspI CCGCGG 1 cut(s) 2562
LguI GCTCTTC 1 cut(s) 711
LmnI GCTCC 4 cut(s) 31, 509, 2144, 2425
Lsp1109I GCAGC 8 cut(s) 226, 1474, 1622, 1861, 1883, 2071, 2336, 2463
LweI GCATC 5 cut(s) 194, 1477, 1485, 1846, 2566
MaeI CTAG 6 cut(s) 722, 1353, 1361, 1662, 1712, 2718
MaeII ACGT 3 cut(s) 1104, 1327, 2462
MflI RGATCY 3 cut(s) 913, 2002, 2691
MhlI GDGCHC 2 cut(s) 1762, 2538
MlsI TGGCCA 1 cut(s) 2498
MluNI TGGCCA 1 cut(s) 2498
MlyI GAGTC 5 cut(s) 392, 600, 1425, 1715, 2257
MmeI TCCRAC 2 cut(s) 379, 2598
Mox20I TGGCCA 1 cut(s) 2498
Mph1103I ATGCAT 2 cut(s) 793, 1492
MroI TCCGGA 2 cut(s) 1174, 1615
MroNI GCCGGC 2 cut(s) 406, 2385
MroXI GAANNNNTTC 2 cut(s) 573, 1197
MscI TGGCCA 1 cut(s) 2498
MslI CAYNNNNRTG 5 cut(s) 537, 1161, 1485, 1617, 1848
Msp20I TGGCCA 1 cut(s) 2498
MspA1I CMGCKG 4 cut(s) 1874, 2476, 2528, 2561
MspI CCGG 9 cut(s) 407, 506, 1175, 1349, 1616, 1725, 2386, 2674, 2684
MspR9I CCNGG 8 cut(s) 194, 506, 507, 579, 678, 2194, 2245, 2727
Mva1269I GAATGC 4 cut(s) 7, 1170, 1867, 2450
MvaI CCWGG 6 cut(s) 194, 579, 678, 2194, 2245, 2727
MvnI CGCG 5 cut(s) 838, 1879, 2042, 2211, 2561
MwoI GCNNNNNNNGC 7 cut(s) 1641, 1871, 2217, 2257, 2321, 2395, 2630
NaeI GCCGGC 2 cut(s) 408, 2387
NciI CCSGG 2 cut(s) 506, 507
NcoI CCATGG 2 cut(s) 1095, 1998
NdeI CATATG 1 cut(s) 2080
NgoMIV GCCGGC 2 cut(s) 406, 2385
NheI GCTAGC 1 cut(s) 1352
NlaIV GGNNCC 5 cut(s) 825, 826, 915, 988, 2522
NmuCI GTSAC 7 cut(s) 368, 427, 559, 1768, 2043, 2267, 2552
NsiI ATGCAT 2 cut(s) 793, 1492
NspV TTCGAA 2 cut(s) 1089, 1201
OliI CACNNNNGTG 1 cut(s) 1161
PciSI GCTCTTC 1 cut(s) 711
PcsI WCGNNNNNNNCGW 2 cut(s) 282, 486
PctI GAATGC 4 cut(s) 7, 1170, 1867, 2450
PdiI GCCGGC 2 cut(s) 408, 2387
PdmI GAANNNNTTC 2 cut(s) 573, 1197
PfeI GAWTC 8 cut(s) 61, 301, 811, 1822, 2033, 2177, 2282, 2358
PflFI GACNNNGTC 2 cut(s) 48, 776
PinAI ACCGGT 1 cut(s) 2673
PleI GAGTC 5 cut(s) 391, 599, 1425, 1715, 2257
PpsI GAGTC 5 cut(s) 391, 599, 1425, 1715, 2257
PpuMI RGGWCCY 2 cut(s) 824, 1039
Psp124BI GAGCTC 2 cut(s) 1762, 2538
Psp1406I AACGTT 1 cut(s) 2462
Psp5II RGGWCCY 2 cut(s) 824, 1039
Psp6I CCWGG 6 cut(s) 192, 577, 676, 2192, 2243, 2725
PspGI CCWGG 6 cut(s) 192, 577, 676, 2192, 2243, 2725
PspN4I GGNNCC 5 cut(s) 825, 826, 915, 988, 2522
PspPI GGNCC 4 cut(s) 824, 986, 1039, 2681
PspPPI RGGWCCY 2 cut(s) 824, 1039
PstI CTGCAG 2 cut(s) 2089, 2592
PstNI CAGNNNCTG 2 cut(s) 1749, 1772
PsuI RGATCY 3 cut(s) 913, 2002, 2691
PsyI GACNNNGTC 2 cut(s) 48, 776
PvuII CAGCTG 2 cut(s) 1874, 2476
RsaI GTAC 6 cut(s) 635, 866, 1107, 1502, 1964, 2345
RsaNI GTAC 6 cut(s) 634, 865, 1106, 1501, 1963, 2344
RseI CAYNNNNRTG 5 cut(s) 537, 1161, 1485, 1617, 1848
Rsr2I CGGWCCG 1 cut(s) 2681
RsrII CGGWCCG 1 cut(s) 2681
SacI GAGCTC 2 cut(s) 1762, 2538
SacII CCGCGG 1 cut(s) 2562
SalI GTCGAC 1 cut(s) 385
SapI GCTCTTC 1 cut(s) 711
Sau96I GGNCC 4 cut(s) 824, 986, 1039, 2681
ScaI AGTACT 1 cut(s) 2345
SchI GAGTC 5 cut(s) 392, 600, 1425, 1715, 2257
ScrFI CCNGG 8 cut(s) 194, 506, 507, 579, 678, 2194, 2245, 2727
SduI GDGCHC 2 cut(s) 1762, 2538
SfaNI GCATC 5 cut(s) 194, 1477, 1485, 1846, 2566
SfcI CTRYAG 3 cut(s) 698, 2085, 2588
Sfr303I CCGCGG 1 cut(s) 2562
SfuI TTCGAA 2 cut(s) 1089, 1201
SgrBI CCGCGG 1 cut(s) 2562
SinI GGWCC 4 cut(s) 824, 986, 1039, 2681
SmaI CCCGGG 1 cut(s) 507
SmiMI CAYNNNNRTG 5 cut(s) 537, 1161, 1485, 1617, 1848
SmlI CTYRAG 1 cut(s) 1239
SmoI CTYRAG 1 cut(s) 1239
SpeI ACTAGT 1 cut(s) 1711
SsiI CCGC 9 cut(s) 335, 1510, 1790, 2209, 2371, 2389, 2526, 2559, 2561
SspMI CTAG 6 cut(s) 722, 1353, 1361, 1662, 1712, 2718
SstI GAGCTC 2 cut(s) 1762, 2538
StyD4I CCNGG 8 cut(s) 192, 504, 505, 577, 676, 2192, 2243, 2725
StyI CCWWGG 4 cut(s) 1069, 1095, 1666, 1998
TaiI ACGT 3 cut(s) 1107, 1330, 2465
TaqI TCGA 9 cut(s) 276, 299, 386, 480, 567, 589, 1089, 1201, 2348
TaqII GACCGA 1 cut(s) 1380
TatI WGTACW 3 cut(s) 633, 1962, 2343
TauI GCSGC 1 cut(s) 1793
TfiI GAWTC 8 cut(s) 61, 301, 811, 1822, 2033, 2177, 2282, 2358
TscAI CASTG 4 cut(s) 544, 1239, 1754, 1773
TseFI GTSAC 7 cut(s) 368, 427, 559, 1768, 2043, 2267, 2552
TseI GCWGC 9 cut(s) 4, 214, 1462, 1635, 1871, 1874, 2084, 2324, 2476
Tsp45I GTSAC 7 cut(s) 368, 427, 559, 1768, 2043, 2267, 2552
TspGWI ACGGA 5 cut(s) 42, 926, 1647, 2238, 2588
TspMI CCCGGG 1 cut(s) 505
TspRI CASTG 4 cut(s) 544, 1239, 1754, 1773
Tth111I GACNNNGTC 2 cut(s) 48, 776
VpaK11BI GGWCC 4 cut(s) 824, 986, 1039, 2681
XagI CCTNNNNNAGG 2 cut(s) 582, 2403
XapI RAATTY 5 cut(s) 704, 1226, 1283, 1340, 2745
XmaI CCCGGG 1 cut(s) 505
XmiI GTMKAC 1 cut(s) 386
XmnI GAANNNNTTC 2 cut(s) 573, 1197
XspI CTAG 6 cut(s) 722, 1353, 1361, 1662, 1712, 2718
ZrmI AGTACT 1 cut(s) 2345
Zsp2I ATGCAT 2 cut(s) 793, 1492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.