pycom04g18050

Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0000763
Physical Location & Seq
Reverse (-)
20207377 .. 20210884
3508 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g18050.6

Sequence Viewer

Length: 2448 bp
ATGCTGCATTCCAAGTATCCCACGTCGTCCCAGGATACCACCGTTGATACGCAACCCAAAAATGAGGGCAAGAAGATCAAGGGGAGAGTGGTGTTGATGAAGAAGGTTGTTCTCGATGTGAACGACCTTAAAGCTTCGATTCTTGATCGTGTTGACGAGCTGTTAGGCAAAGCGGTTTCTCTAAGGCTCATTAGTTCGGTTAATGGTGACCCTGAAAATGAGTTGAAAGGAAAAGTTGGAAAGCCAGCATACTTGGAGAACTGGGTGACCAAAATCACCCCCTTGACAGCAGGGGAGGCTGCATTTGATGTTACTTTTGATTGGGAAAAGGAAATTGGCGTCCCGGGAGCTTTCGTAGTTAGAAATGAGCATCACAGTGAGTTCTACTTGAAGACTCTCACACTCGAAGATGTTCCTGGCGAAGGTCGAGTCCACTTTGTTTGCAACTCATGGGTGTATCCTGCTGACAAGTACAAAAAAGACCGTGTTTTTTTCTCTAACAAGACCTATCTTTCAAGTGATACACCAAAGCCACTACAGAAATTTAGAGAAGAAGAGCTAGTAAACCTGCGAGGAGACGATGAAGAAAGAGGAGAGCTTCAGGAATGGGACAGGGTCTATGACTATGCATACTACAACGATTTGGGGAATCCAGATAAGGGTCCCGAATATGCACGTCCAGTTCTTGGAGGGTCTATTGAGTACCCATATCCTCGCCGCGGAAGAACAGGAAGACCAGCAACAAAAACAGATCCCAACACCGAGAGCAGGCTGAAGCTTATCCAGATCTTAAATGTATATGTTCCAAGAGACGAACGATTTGGACCTGTAAAGATGTCAGACTTGCTTGCTTATGTCCTGAAATCTATCCCTCAGGTCCTTAAACCTGAGATAAGAGATCTACTCGTTGGTAACAAAAACGAGTTCGAAAGCATGGAAGAAGTACTTAAACTCTATGAAGGAGGATTAGAATTGCCTGATGGGATACTAAAACACATTAGTGATAGCACACCCGAGGAGATGTTCAAGGAGCTTTTTCGAACTGATGGCGAAAGGTTCCTAAAATTTCCAGTGCCTCAAGTGATAAAAGAGGATAAGTCGGCATGGAGAACTGACGAAGAATTTGCAAGAGAACTACTGGCTGGAGTAAATCCTGTCACTATAAGTCGTCTCCAAGAATTTCCACCGTCTAGCGAGCTAGACCGAGAAGCATATGGGGATCAAACAAGTCAACTAACCAAAGAACACATAGAACATAACTTAAATGGACTCACCATAGATGAGGCAATCATGAGCAACAAGCTATTCATATTAGACCACCACGATGCATGGATGCCGTACCTGAGGCGGATAAACACAACTTCCACAAAAGCTTATGCAAGCAGAACACTCCTTTTCTTGAACAATGATGGGACTTTAAAGCCAATAGCTATTGAACTCAGCTTGCCACATCCGGATGGAGATCAATTTGGTTGCATTAGCAAAGTCTATACTCCGTCTAGCCAAGGCGTCGAGAGTTCCATTTGGCAACTTGCTAAAGGTTATGCACTTGTAAATGACTCCGGCTGTCATCAGCTTTTCAGCCACTGGTTGAGAGCTCATGCAGTGACTGAGCCATTTGTGATAGCGGCAAATAGGCAATTGAGCGTGCTTCACCCGATTCACAAACTTCTGCATCCTCATTTTCGTGGGACGATGAATGCAAACGCATCTGCACGACAGGTTCTCACAAACGCAGGTGGAGTAATAGAGGAAATAATATTTGCTTCCAAGTTTTCGATGGAATGGTCATCAGCAATGTACAAGGACTGGACTTTTCCTGATCAAGCGCTCCCTGCGGATCTGATCAAAAGAGGAGTGGCGGTTGAAGATTCAAGCGTGTCACACGGTGCGCGTTTACTGATAGAGGACTATCCATATGCTGCAGACGGACTTGAATTATGGTCTGCAATTAAAACATGGGTTAAAGACTATTGCTCCTTCTACTACAAAAATGGTTTTGGATCGATCAAATGGTTCAAAATGATTCGGAACTCCAATCGTGGTGGAAGGAACTCCGCGAGCCGGAGGTTCATGCCTGATGAAGGCACTCCAGAGTATGAGGAGCTCAGGACAAACCCTGACAAGGCATTCTTGAAAACGTTTGCACCTCAGCTGCCGACACTTCTTGGAATGGCCACGGTAGAAATCTTGTCAAGGCACCCCACTGATGAGCTCTATCTCGGACAGCGTGACACCGCGGAGTGGACAACGGATGCAGACATATTGCAGGCTTCTGAGGATTTTAAAAAGAATTTGGAAGCGATTGAGGCCAAAATTGAAAAGATGAACAAGGATGAGAGATTGAAAAACCGGTTCGGACCGGCCAAGATCCCTTACACTTTGCTGTATCCTTCTAGTGAGCCTGGACTTACTAACAAGGGAGTTCCCACCAGTATCAACATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00591 map00591
Pfam Domains
Protein Families
Lipoxygenase PLAT

Protein Analysis

816

Amino Acids

92.5

Weight (kDa)

5.81

Isoelectric Point (pI)

41.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1727
Acc36I ACCTGC 2 cut(s) 576, 1727
AccB1I GGYRCC 1 cut(s) 2199
AccB7I CCANNNNNTGG 1 cut(s) 686
AccII CGCG 4 cut(s) 720, 1894, 2060, 2240
AccIII TCCGGA 1 cut(s) 1453
AclI AACGTT 1 cut(s) 2141
AclWI GGATC 5 cut(s) 746, 1227, 1848, 2011, 2365
AcoI YGGCCR 2 cut(s) 2175, 2364
AcsI RAATTY 5 cut(s) 542, 1064, 1121, 1178, 2293
AcuI CTGAAG 2 cut(s) 584, 794
AcyI GRCGYC 2 cut(s) 339, 1509
AdeI CACNNNGTG 1 cut(s) 1889
AfaI GTAC 5 cut(s) 473, 704, 945, 1340, 1802
AfeI AGCGCT 1 cut(s) 1830
AfiI CCNNNNNNNGG 9 cut(s) 422, 659, 686, 719, 768, 2064, 2084, 2125, 2244
AgeI ACCGGT 1 cut(s) 2352
AjiI CACGTC 2 cut(s) 24, 677
AjnI CCWGG 3 cut(s) 30, 415, 2404
AjuI GAANNNNNNNTTGG 2 cut(s) 318, 350
AleI CACNNNNGTG 1 cut(s) 999
Alw21I GWGCWC 3 cut(s) 1600, 2109, 2217
Alw26I GTCTC 3 cut(s) 570, 804, 1175
AlwI GGATC 5 cut(s) 746, 1227, 1848, 2011, 2365
AlwNI CAGNNNCTG 2 cut(s) 1587, 1610
Ama87I CYCGRG 2 cut(s) 343, 1013
Aor13HI TCCGGA 1 cut(s) 1453
Aor51HI AGCGCT 1 cut(s) 1830
AoxI GGCC 3 cut(s) 2175, 2310, 2364
ApeKI GCWGC 4 cut(s) 4, 299, 1922, 2155
ApoI RAATTY 5 cut(s) 542, 1064, 1121, 1178, 2293
ArsI GACNNNNNNTTYG 6 cut(s) 414, 446, 803, 835, 1106, 1138
AsiGI ACCGGT 1 cut(s) 2352
Asp700I GAANNNNTTC 1 cut(s) 411
AspLEI GCGC 2 cut(s) 1831, 1894
AspS9I GGNCC 4 cut(s) 662, 824, 877, 2360
AsuC2I CCSGG 2 cut(s) 344, 345
AsuHPI GGTGA 5 cut(s) 218, 268, 277, 1264, 1646
AsuII TTCGAA 2 cut(s) 927, 1039
AvaI CYCGRG 2 cut(s) 343, 1013
AvaII GGWCC 4 cut(s) 662, 824, 877, 2360
AxyI CCTNAGG 2 cut(s) 873, 1343
BaeI ACNNNNGTAYC 2 cut(s) 27, 60
BalI TGGCCA 1 cut(s) 2177
BanI GGYRCC 1 cut(s) 2199
BanII GRGCYC 3 cut(s) 1600, 2109, 2217
BbsI GAAGAC 2 cut(s) 398, 739
Bbv12I GWGCWC 3 cut(s) 1600, 2109, 2217
BbvCI CCTCAGC 1 cut(s) 2151
BbvI GCAGC 3 cut(s) 286, 1909, 2142
BccI CCATC 5 cut(s) 974, 1040, 1403, 1451, 1774
BceAI ACGGC 1 cut(s) 1321
BcgI CGANNNNNNTGC 2 cut(s) 1106, 1140
BciT130I CCWGG 3 cut(s) 32, 417, 2406
BciVI GTATCC 5 cut(s) 27, 28, 468, 978, 2400
BclI TGATCA 2 cut(s) 1822, 1845
BcnI CCSGG 2 cut(s) 344, 345
BcoDI GTCTC 3 cut(s) 570, 804, 1175
BfaI CTAG 5 cut(s) 560, 1191, 1199, 1500, 2397
BfmI CTRYAG 2 cut(s) 536, 1923
BfoI RGCGCY 1 cut(s) 1832
BfuAI ACCTGC 2 cut(s) 576, 1727
BfuI GTATCC 5 cut(s) 27, 28, 468, 978, 2400
BglII AGATCT 2 cut(s) 786, 898
BisI GCNGC 6 cut(s) 5, 300, 718, 1629, 1923, 2156
BlsI GCNGC 6 cut(s) 6, 301, 719, 1630, 1924, 2157
BmcAI AGTACT 1 cut(s) 945
Bme1390I CCNGG 5 cut(s) 32, 344, 345, 417, 2406
Bme18I GGWCC 4 cut(s) 662, 824, 877, 2360
BmeT110I CYCGRG 2 cut(s) 343, 1013
BmgBI CACGTC 2 cut(s) 24, 677
BmgT120I GGNCC 4 cut(s) 662, 824, 877, 2360
BmiI GGNNCC 4 cut(s) 663, 664, 1058, 2201
BmrFI CCNGG 5 cut(s) 32, 344, 345, 417, 2406
BmrI ACTGGG 1 cut(s) 271
BmsI GCATC 6 cut(s) 379, 1315, 1323, 1684, 1718, 2245
BmuI ACTGGG 1 cut(s) 271
BpiI GAAGAC 2 cut(s) 398, 739
BplI GAGNNNNNCTC 2 cut(s) 888, 920
BpmI CTGGAG 2 cut(s) 1164, 2076
Bpu10I CCTNAGC 2 cut(s) 2108, 2151
Bpu14I TTCGAA 2 cut(s) 927, 1039
BpuEI CTTGAG 1 cut(s) 1062
BpuMI CCSGG 2 cut(s) 344, 345
Bsa29I ATCGAT 1 cut(s) 2006
BsaBI GATNNNNATC 1 cut(s) 1461
BsaHI GRCGYC 2 cut(s) 339, 1509
BsaJI CCNNGG 7 cut(s) 30, 343, 718, 1014, 1504, 2178, 2238
BsaWI WCCGGW 2 cut(s) 1453, 2352
BsaXI ACNNNNNCTCC 6 cut(s) 339, 369, 1848, 1878, 1961, 1991
Bsc4I CCNNNNNNNGG 9 cut(s) 422, 659, 686, 719, 768, 2064, 2084, 2125, 2244
Bse118I RCCGGY 2 cut(s) 2352, 2362
Bse1I ACTGG 7 cut(s) 266, 680, 1070, 1143, 1592, 1814, 2433
Bse21I CCTNAGG 2 cut(s) 873, 1343
Bse3DI GCAATG 1 cut(s) 1803
Bse8I GATNNNNATC 1 cut(s) 1461
BseAI TCCGGA 1 cut(s) 1453
BseBI CCWGG 3 cut(s) 32, 417, 2406
BseCI ATCGAT 1 cut(s) 2006
BseDI CCNNGG 7 cut(s) 30, 343, 718, 1014, 1504, 2178, 2238
BseGI GGATG 6 cut(s) 1338, 1450, 1462, 1675, 2260, 2341
BseJI GATNNNNATC 1 cut(s) 1461
BseLI CCNNNNNNNGG 9 cut(s) 422, 659, 686, 719, 768, 2064, 2084, 2125, 2244
BseMI GCAATG 1 cut(s) 1803
BseMII CTCAG 8 cut(s) 879, 887, 1334, 1453, 1602, 2122, 2165, 2268
BseNI ACTGG 7 cut(s) 266, 680, 1070, 1143, 1592, 1814, 2433
BseRI GAGGAG 5 cut(s) 588, 606, 1031, 1869, 2117
BseXI GCAGC 3 cut(s) 286, 1909, 2142
BsgI GTGCAG 1 cut(s) 1698
Bsh1236I CGCG 4 cut(s) 720, 1894, 2060, 2240
BshFI GGCC 3 cut(s) 2177, 2312, 2366
BshNI GGYRCC 1 cut(s) 2199
BshTI ACCGGT 1 cut(s) 2352
BshVI ATCGAT 1 cut(s) 2006
BsiHKAI GWGCWC 3 cut(s) 1600, 2109, 2217
BsiHKCI CYCGRG 2 cut(s) 343, 1013
BsiSI CCGG 6 cut(s) 344, 1454, 1563, 2065, 2353, 2363
BslFI GGGAC 6 cut(s) 13, 326, 623, 648, 1426, 1705
BslI CCNNNNNNNGG 9 cut(s) 422, 659, 686, 719, 768, 2064, 2084, 2125, 2244
BsmAI GTCTC 3 cut(s) 570, 804, 1175
BsmBI CGTCTC 3 cut(s) 570, 804, 1175
BsmFI GGGAC 6 cut(s) 13, 326, 623, 648, 1426, 1705
BsmI GAATGC 3 cut(s) 7, 1705, 2129
BsnI GGCC 3 cut(s) 2177, 2312, 2366
BsoBI CYCGRG 2 cut(s) 343, 1013
Bsp119I TTCGAA 2 cut(s) 927, 1039
Bsp1286I GDGCHC 3 cut(s) 1600, 2109, 2217
Bsp13I TCCGGA 1 cut(s) 1453
Bsp1407I TGTACA 1 cut(s) 1800
BspANI GGCC 3 cut(s) 2177, 2312, 2366
BspCNI CTCAG 8 cut(s) 880, 886, 1335, 1452, 1603, 2121, 2164, 2269
BspDI ATCGAT 1 cut(s) 2006
BspEI TCCGGA 1 cut(s) 1453
BspFNI CGCG 4 cut(s) 720, 1894, 2060, 2240
BspHI TCATGA 1 cut(s) 1290
BspLI GGNNCC 4 cut(s) 663, 664, 1058, 2201
BspMAI CTGCAG 1 cut(s) 1927
BspMI ACCTGC 2 cut(s) 576, 1727
BspPI GGATC 5 cut(s) 746, 1227, 1848, 2011, 2365
BspQI GCTCTTC 1 cut(s) 549
BspT104I TTCGAA 2 cut(s) 927, 1039
BspT107I GGYRCC 1 cut(s) 2199
BsrDI GCAATG 1 cut(s) 1803
BsrFI RCCGGY 2 cut(s) 2352, 2362
BsrGI TGTACA 1 cut(s) 1800
BsrI ACTGG 7 cut(s) 266, 680, 1070, 1143, 1592, 1814, 2433
BssAI RCCGGY 2 cut(s) 2352, 2362
BssECI CCNNGG 7 cut(s) 30, 343, 718, 1014, 1504, 2178, 2238
BssNI GRCGYC 2 cut(s) 339, 1509
BssT1I CCWWGG 1 cut(s) 1504
Bst2UI CCWGG 3 cut(s) 32, 417, 2406
Bst4CI ACNGT 6 cut(s) 43, 377, 485, 1188, 1889, 2182
Bst6I CTCTTC 1 cut(s) 549
BstACI GRCGYC 2 cut(s) 339, 1509
BstAUI TGTACA 1 cut(s) 1800
BstBI TTCGAA 2 cut(s) 927, 1039
BstC8I GCNNGC 9 cut(s) 246, 770, 849, 1196, 1381, 1445, 1649, 2062, 2271
BstDEI CTNAG 9 cut(s) 182, 873, 888, 1343, 1439, 1611, 2108, 2151, 2277
BstDSI CCRYGG 3 cut(s) 718, 2178, 2238
BstEII GGTNACC 2 cut(s) 206, 265
BstENI CCTNNNNNAGG 2 cut(s) 420, 2082
BstF5I GGATG 6 cut(s) 1338, 1450, 1462, 1675, 2260, 2341
BstFNI CGCG 4 cut(s) 720, 1894, 2060, 2240
BstH2I RGCGCY 1 cut(s) 1832
BstHHI GCGC 2 cut(s) 1831, 1894
BstMAI GTCTC 3 cut(s) 570, 804, 1175
BstMWI GCNNNNNNNGC 3 cut(s) 296, 1835, 2309
BstNI CCWGG 3 cut(s) 32, 417, 2406
BstPI GGTNACC 2 cut(s) 206, 265
BstSCI CCNGG 5 cut(s) 30, 342, 343, 415, 2404
BstSFI CTRYAG 2 cut(s) 536, 1923
BstUI CGCG 4 cut(s) 720, 1894, 2060, 2240
BstV1I GCAGC 3 cut(s) 286, 1909, 2142
BstV2I GAAGAC 2 cut(s) 398, 739
BstX2I RGATCY 5 cut(s) 751, 786, 898, 1840, 2370
BstYI RGATCY 5 cut(s) 751, 786, 898, 1840, 2370
Bsu15I ATCGAT 1 cut(s) 2006
Bsu36I CCTNAGG 2 cut(s) 873, 1343
BsuI GTATCC 5 cut(s) 27, 28, 468, 978, 2400
BsuRI GGCC 3 cut(s) 2177, 2312, 2366
BsuTUI ATCGAT 1 cut(s) 2006
BtgI CCRYGG 3 cut(s) 718, 2178, 2238
BtrI CACGTC 2 cut(s) 24, 677
BtsCI GGATG 6 cut(s) 1338, 1450, 1462, 1675, 2260, 2341
BtsI GCAGTG 1 cut(s) 1611
BtsIMutI CAGTG 5 cut(s) 382, 1077, 1585, 1611, 2205
BveI ACCTGC 2 cut(s) 576, 1727
Cac8I GCNNGC 9 cut(s) 246, 770, 849, 1196, 1381, 1445, 1649, 2062, 2271
CaiI CAGNNNCTG 2 cut(s) 1587, 1610
CciI TCATGA 1 cut(s) 1290
CfoI GCGC 2 cut(s) 1831, 1894
Cfr10I RCCGGY 2 cut(s) 2352, 2362
Cfr13I GGNCC 4 cut(s) 662, 824, 877, 2360
Cfr42I CCGCGG 2 cut(s) 721, 2241
Cfr9I CCCGGG 1 cut(s) 343
ClaI ATCGAT 1 cut(s) 2006
CpoI CGGWCCG 1 cut(s) 2360
CseI GACGC 2 cut(s) 328, 1498
Csp6I GTAC 5 cut(s) 472, 703, 944, 1339, 1801
CspAI ACCGGT 1 cut(s) 2352
CspCI CAANNNNNGTGG 4 cut(s) 422, 457, 2026, 2061
CspI CGGWCCG 1 cut(s) 2360
CviAII CATG 8 cut(s) 450, 934, 1104, 1291, 1329, 1601, 1959, 2074
CviQI GTAC 5 cut(s) 472, 703, 944, 1339, 1801
DdeI CTNAG 9 cut(s) 182, 873, 888, 1343, 1439, 1611, 2108, 2151, 2277
DraI TTTAAA 2 cut(s) 1419, 2287
DraIII CACNNNGTG 1 cut(s) 1889
EaeI YGGCCR 2 cut(s) 2175, 2364
Eam1104I CTCTTC 1 cut(s) 549
EarI CTCTTC 1 cut(s) 549
EciI GGCGGA 1 cut(s) 1363
Ecl136II GAGCTC 3 cut(s) 1598, 2107, 2215
Eco130I CCWWGG 1 cut(s) 1504
Eco24I GRGCYC 3 cut(s) 1600, 2109, 2217
Eco47I GGWCC 4 cut(s) 662, 824, 877, 2360
Eco47III AGCGCT 1 cut(s) 1830
Eco53kI GAGCTC 3 cut(s) 1598, 2107, 2215
Eco57I CTGAAG 2 cut(s) 584, 794
Eco81I CCTNAGG 2 cut(s) 873, 1343
Eco88I CYCGRG 2 cut(s) 343, 1013
Eco91I GGTNACC 2 cut(s) 206, 265
EcoICRI GAGCTC 3 cut(s) 1598, 2107, 2215
EcoNI CCTNNNNNAGG 2 cut(s) 420, 2082
EcoO109I RGGNCCY 2 cut(s) 662, 877
EcoO65I GGTNACC 2 cut(s) 206, 265
EcoRII CCWGG 3 cut(s) 30, 415, 2404
EcoT14I CCWWGG 1 cut(s) 1504
EcoT22I ATGCAT 2 cut(s) 631, 1330
EcoT38I GRGCYC 3 cut(s) 1600, 2109, 2217
ErhI CCWWGG 1 cut(s) 1504
Esp3I CGTCTC 3 cut(s) 570, 804, 1175
FaeI CATG 8 cut(s) 453, 937, 1107, 1294, 1332, 1604, 1962, 2077
FalI AAGNNNNNCTT 4 cut(s) 930, 962, 2117, 2149
FaqI GGGAC 6 cut(s) 13, 326, 623, 648, 1426, 1705
FatI CATG 8 cut(s) 449, 933, 1103, 1290, 1328, 1600, 1958, 2073
FauNDI CATATG 2 cut(s) 1213, 1918
FbaI TGATCA 2 cut(s) 1822, 1845
Fnu4HI GCNGC 6 cut(s) 5, 300, 718, 1629, 1923, 2156
FokI GGATG 6 cut(s) 1345, 1437, 1469, 1662, 2267, 2348
FriOI GRGCYC 3 cut(s) 1600, 2109, 2217
Fsp4HI GCNGC 6 cut(s) 5, 300, 718, 1629, 1923, 2156
FspBI CTAG 5 cut(s) 560, 1191, 1199, 1500, 2397
GlaI GCGC 2 cut(s) 1830, 1893
GluI GCNGC 6 cut(s) 5, 300, 718, 1629, 1923, 2156
GsuI CTGGAG 2 cut(s) 1164, 2076
HaeII RGCGCY 1 cut(s) 1832
HaeIII GGCC 3 cut(s) 2177, 2312, 2366
HapII CCGG 6 cut(s) 344, 1454, 1563, 2065, 2353, 2363
HgaI GACGC 2 cut(s) 328, 1498
HhaI GCGC 2 cut(s) 1831, 1894
Hin1I GRCGYC 2 cut(s) 339, 1509
Hin1II CATG 8 cut(s) 453, 937, 1107, 1294, 1332, 1604, 1962, 2077
Hin6I GCGC 2 cut(s) 1829, 1892
HinP1I GCGC 2 cut(s) 1829, 1892
HincII GTYRAC 2 cut(s) 154, 1232
HindII GTYRAC 2 cut(s) 154, 1232
HindIII AAGCTT 3 cut(s) 132, 776, 1371
HinfI GANTC 9 cut(s) 139, 394, 429, 649, 1269, 1559, 1660, 1871, 2026
HpaII CCGG 6 cut(s) 344, 1454, 1563, 2065, 2353, 2363
HphI GGTGA 5 cut(s) 218, 268, 277, 1264, 1646
Hpy166II GTNNAC 7 cut(s) 121, 154, 433, 565, 1232, 1898, 2247
Hpy188I TCNGA 6 cut(s) 841, 1845, 2031, 2225, 2278, 2360
Hpy8I GTNNAC 7 cut(s) 121, 154, 433, 565, 1232, 1898, 2247
Hpy99I CGWCG 2 cut(s) 28, 1514
HpyAV CCTTC 7 cut(s) 97, 416, 953, 1990, 2043, 2078, 2403
HpyCH4III ACNGT 6 cut(s) 43, 377, 485, 1188, 1889, 2182
HpyCH4IV ACGT 3 cut(s) 23, 676, 2141
HpyF10VI GCNNNNNNNGC 3 cut(s) 296, 1835, 2309
HpyF3I CTNAG 9 cut(s) 182, 873, 888, 1343, 1439, 1611, 2108, 2151, 2277
HpySE526I ACGT 3 cut(s) 23, 676, 2141
Hsp92I GRCGYC 2 cut(s) 339, 1509
Hsp92II CATG 8 cut(s) 453, 937, 1107, 1294, 1332, 1604, 1962, 2077
HspAI GCGC 2 cut(s) 1829, 1892
KflI GGGWCCC 1 cut(s) 662
Kpn2I TCCGGA 1 cut(s) 1453
Ksp22I TGATCA 2 cut(s) 1822, 1845
KspI CCGCGG 2 cut(s) 721, 2241
LguI GCTCTTC 1 cut(s) 549
LmnI GCTCC 5 cut(s) 347, 1030, 1836, 1982, 2104
Lsp1109I GCAGC 3 cut(s) 286, 1909, 2142
LweI GCATC 6 cut(s) 379, 1315, 1323, 1684, 1718, 2245
MaeI CTAG 5 cut(s) 560, 1191, 1199, 1500, 2397
MaeII ACGT 3 cut(s) 23, 676, 2141
MaeIII GTNAC 8 cut(s) 206, 265, 310, 911, 1156, 1606, 1881, 2231
MfeI CAATTG 1 cut(s) 1640
MflI RGATCY 5 cut(s) 751, 786, 898, 1840, 2370
MhlI GDGCHC 3 cut(s) 1600, 2109, 2217
MlsI TGGCCA 1 cut(s) 2177
MluNI TGGCCA 1 cut(s) 2177
MlyI GAGTC 4 cut(s) 388, 438, 1263, 1553
MmeI TCCRAC 1 cut(s) 217
Mox20I TGGCCA 1 cut(s) 2177
Mph1103I ATGCAT 2 cut(s) 631, 1330
MroI TCCGGA 1 cut(s) 1453
MroXI GAANNNNTTC 1 cut(s) 411
MscI TGGCCA 1 cut(s) 2177
MslI CAYNNNNRTG 5 cut(s) 375, 999, 1323, 1455, 1686
Msp20I TGGCCA 1 cut(s) 2177
MspA1I CMGCKG 3 cut(s) 720, 2155, 2240
MspI CCGG 6 cut(s) 344, 1454, 1563, 2065, 2353, 2363
MspR9I CCNGG 5 cut(s) 32, 344, 345, 417, 2406
MunI CAATTG 1 cut(s) 1640
Mva1269I GAATGC 3 cut(s) 7, 1705, 2129
MvaI CCWGG 3 cut(s) 32, 417, 2406
MvnI CGCG 4 cut(s) 720, 1894, 2060, 2240
MwoI GCNNNNNNNGC 3 cut(s) 296, 1835, 2309
NciI CCSGG 2 cut(s) 344, 345
NdeI CATATG 2 cut(s) 1213, 1918
NlaIII CATG 8 cut(s) 453, 937, 1107, 1294, 1332, 1604, 1962, 2077
NlaIV GGNNCC 4 cut(s) 663, 664, 1058, 2201
NmuCI GTSAC 6 cut(s) 206, 265, 1156, 1606, 1881, 2231
NsiI ATGCAT 2 cut(s) 631, 1330
NspV TTCGAA 2 cut(s) 927, 1039
OliI CACNNNNGTG 1 cut(s) 999
PagI TCATGA 1 cut(s) 1290
PaqCI CACCTGC 1 cut(s) 1727
PciSI GCTCTTC 1 cut(s) 549
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PctI GAATGC 3 cut(s) 7, 1705, 2129
PdmI GAANNNNTTC 1 cut(s) 411
PfeI GAWTC 5 cut(s) 139, 649, 1660, 1871, 2026
PflFI GACNNNGTC 1 cut(s) 614
PflMI CCANNNNNTGG 1 cut(s) 686
PinAI ACCGGT 1 cut(s) 2352
PkrI GCNGC 6 cut(s) 6, 301, 719, 1630, 1924, 2157
PleI GAGTC 4 cut(s) 388, 437, 1263, 1553
PpsI GAGTC 4 cut(s) 388, 437, 1263, 1553
PpuMI RGGWCCY 2 cut(s) 662, 877
Psp124BI GAGCTC 3 cut(s) 1600, 2109, 2217
Psp1406I AACGTT 1 cut(s) 2141
Psp5II RGGWCCY 2 cut(s) 662, 877
Psp6I CCWGG 3 cut(s) 30, 415, 2404
PspEI GGTNACC 2 cut(s) 206, 265
PspGI CCWGG 3 cut(s) 30, 415, 2404
PspN4I GGNNCC 4 cut(s) 663, 664, 1058, 2201
PspPI GGNCC 4 cut(s) 662, 824, 877, 2360
PspPPI RGGWCCY 2 cut(s) 662, 877
PstI CTGCAG 1 cut(s) 1927
PstNI CAGNNNCTG 2 cut(s) 1587, 1610
PsuI RGATCY 5 cut(s) 751, 786, 898, 1840, 2370
PsyI GACNNNGTC 1 cut(s) 614
PvuII CAGCTG 1 cut(s) 2155
RsaI GTAC 5 cut(s) 473, 704, 945, 1340, 1802
RsaNI GTAC 5 cut(s) 472, 703, 944, 1339, 1801
RseI CAYNNNNRTG 5 cut(s) 375, 999, 1323, 1455, 1686
Rsr2I CGGWCCG 1 cut(s) 2360
RsrII CGGWCCG 1 cut(s) 2360
SacI GAGCTC 3 cut(s) 1600, 2109, 2217
SacII CCGCGG 2 cut(s) 721, 2241
SapI GCTCTTC 1 cut(s) 549
SatI GCNGC 6 cut(s) 5, 300, 718, 1629, 1923, 2156
Sau96I GGNCC 4 cut(s) 662, 824, 877, 2360
ScaI AGTACT 1 cut(s) 945
SchI GAGTC 4 cut(s) 388, 438, 1263, 1553
ScrFI CCNGG 5 cut(s) 32, 344, 345, 417, 2406
SduI GDGCHC 3 cut(s) 1600, 2109, 2217
SfaNI GCATC 6 cut(s) 379, 1315, 1323, 1684, 1718, 2245
SfcI CTRYAG 2 cut(s) 536, 1923
Sfr303I CCGCGG 2 cut(s) 721, 2241
SfuI TTCGAA 2 cut(s) 927, 1039
SgrBI CCGCGG 2 cut(s) 721, 2241
SinI GGWCC 4 cut(s) 662, 824, 877, 2360
SmaI CCCGGG 1 cut(s) 345
SmiMI CAYNNNNRTG 5 cut(s) 375, 999, 1323, 1455, 1686
SmlI CTYRAG 1 cut(s) 1077
SmoI CTYRAG 1 cut(s) 1077
SspI AATATT 1 cut(s) 1761
SspMI CTAG 5 cut(s) 560, 1191, 1199, 1500, 2397
SstI GAGCTC 3 cut(s) 1600, 2109, 2217
StyD4I CCNGG 5 cut(s) 30, 342, 343, 415, 2404
StyI CCWWGG 1 cut(s) 1504
TaaI ACNGT 6 cut(s) 43, 377, 485, 1188, 1889, 2182
TaiI ACGT 3 cut(s) 26, 679, 2144
TaqI TCGA 9 cut(s) 114, 137, 405, 427, 927, 1039, 1512, 1778, 2006
TaqII GACCGA 1 cut(s) 1218
TatI WGTACW 3 cut(s) 471, 943, 1800
TauI GCSGC 2 cut(s) 720, 1631
TfiI GAWTC 5 cut(s) 139, 649, 1660, 1871, 2026
TscAI CASTG 5 cut(s) 382, 1077, 1592, 1611, 2212
TseFI GTSAC 6 cut(s) 206, 265, 1156, 1606, 1881, 2231
TseI GCWGC 4 cut(s) 4, 299, 1922, 2155
Tsp45I GTSAC 6 cut(s) 206, 265, 1156, 1606, 1881, 2231
TspDTI ATGAA 8 cut(s) 113, 597, 972, 1297, 1712, 2062, 2097, 2342
TspGWI ACGGA 3 cut(s) 1485, 1944, 2267
TspMI CCCGGG 1 cut(s) 343
TspRI CASTG 5 cut(s) 382, 1077, 1592, 1611, 2212
Tth111I GACNNNGTC 1 cut(s) 614
Van91I CCANNNNNTGG 1 cut(s) 686
VpaK11BI GGWCC 4 cut(s) 662, 824, 877, 2360
XagI CCTNNNNNAGG 2 cut(s) 420, 2082
XapI RAATTY 5 cut(s) 542, 1064, 1121, 1178, 2293
XcmI CCANNNNNNNNNTGG 1 cut(s) 1777
XmaI CCCGGG 1 cut(s) 343
XmnI GAANNNNTTC 1 cut(s) 411
XspI CTAG 5 cut(s) 560, 1191, 1199, 1500, 2397
ZrmI AGTACT 1 cut(s) 945
Zsp2I ATGCAT 2 cut(s) 631, 1330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.