MD04G1211300.v1.1

Protein of unknown function (DUF679)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001834
Physical Location & Seq
Reverse (-)
29573130 .. 29573741
612 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1211300.v1.1.491

Sequence Viewer

Length: 612 bp
ATGGCTGAGGTACTATCTTCCGAGCAACCACGCCCACCACCTTCCCCTCCCACCATATTAAAGCACAGCTTGAACCAGTACGCCGTCCACCAAACCCTAGCCAGTGCGGCCAACCTCACTAACCTTCTACCTACCAGCACAGTCCTCGCATTTCACACCCTCATCCCTACTTTGTCCAACAATGGTTCTTGCCAACTTTTCAATAAGTACCTGACAACATGTGTCATTACATTTTGTGCTCTCCTTTGTTTCTTATCTTCGTTTACTGATAGCTTCATGGACAACGACCGAAAACTCTACTATGGAATCGCCACGTTCAAAGGCATGTACATTTTCAACTGCAGTAACGCCGAGGACATGGATAGGGACTTGAAGAATTATAAAATAAAGTTTATAGATTTTGTTCACGCCTTTATGTCACTATTTGTGTTCTTGGTCTTTGCTCTTTGCAACTTCAATGTGCAAAGTTGCTTCTTTTCTGAAGCGGGAATAAGTGAACTAACAATGAATTTGCCTCTCGGAGCTGGGATTTTGTCGAGCTTCTTGTTCACAATCTTTCCGACTACTCGTAGAGGGATCGGATACGCAGACATGATGCCTCGGGCATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF679

Protein Analysis

204

Amino Acids

22.7

Weight (kDa)

6.87

Isoelectric Point (pI)

37.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 35 - 195 5.9e-60 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 381
AciI CCGC 2 cut(s) 107, 485
AclWI GGATC 1 cut(s) 584
AcoI YGGCCR 1 cut(s) 108
AcsI RAATTY 1 cut(s) 508
AcuI CTGAAG 1 cut(s) 501
AfaI GTAC 4 cut(s) 12, 80, 209, 329
AflIII ACRYGT 1 cut(s) 218
AgsI TTSAA 6 cut(s) 73, 202, 319, 337, 373, 457
AluBI AGCT 4 cut(s) 69, 273, 524, 540
AluI AGCT 4 cut(s) 69, 273, 524, 540
Alw21I GWGCWC 1 cut(s) 241
AlwI GGATC 1 cut(s) 584
Ama87I CYCGRG 1 cut(s) 600
AoxI GGCC 1 cut(s) 108
ApoI RAATTY 1 cut(s) 508
AvaI CYCGRG 1 cut(s) 600
Bbv12I GWGCWC 1 cut(s) 241
BbvCI CCTCAGC 1 cut(s) 6
BceAI ACGGC 1 cut(s) 68
BcgI CGANNNNNNTGC 2 cut(s) 127, 161
BciVI GTATCC 1 cut(s) 575
BfaI CTAG 1 cut(s) 98
BfmI CTRYAG 1 cut(s) 340
BfuI GTATCC 1 cut(s) 575
BglI GCCNNNNNGGC 1 cut(s) 107
BisI GCNGC 1 cut(s) 108
BlsI GCNGC 1 cut(s) 109
BmeT110I CYCGRG 1 cut(s) 600
BmsI GCATC 1 cut(s) 585
Bpu10I CCTNAGC 1 cut(s) 6
BsaJI CCNNGG 2 cut(s) 351, 599
Bse1I ACTGG 2 cut(s) 76, 102
BseDI CCNNGG 2 cut(s) 351, 599
BseGI GGATG 1 cut(s) 162
BseNI ACTGG 2 cut(s) 76, 102
BseYI CCCAGC 1 cut(s) 524
Bsh1285I CGRYCG 1 cut(s) 289
BshFI GGCC 1 cut(s) 110
BsiEI CGRYCG 1 cut(s) 289
BsiHKAI GWGCWC 1 cut(s) 241
BsiHKCI CYCGRG 1 cut(s) 600
BslFI GGGAC 1 cut(s) 380
BsmFI GGGAC 1 cut(s) 380
BsnI GGCC 1 cut(s) 110
BsoBI CYCGRG 1 cut(s) 600
Bsp1286I GDGCHC 1 cut(s) 241
Bsp1407I TGTACA 1 cut(s) 327
Bsp143I GATC 1 cut(s) 576
BspACI CCGC 2 cut(s) 107, 485
BspANI GGCC 1 cut(s) 110
BspMAI CTGCAG 1 cut(s) 344
BspPI GGATC 1 cut(s) 584
BsrGI TGTACA 1 cut(s) 327
BsrI ACTGG 2 cut(s) 76, 102
BssECI CCNNGG 2 cut(s) 351, 599
BssMI GATC 1 cut(s) 576
Bst4CI ACNGT 1 cut(s) 142
BstAUI TGTACA 1 cut(s) 327
BstDEI CTNAG 1 cut(s) 6
BstF5I GGATG 1 cut(s) 162
BstKTI GATC 1 cut(s) 579
BstMBI GATC 1 cut(s) 576
BstMCI CGRYCG 1 cut(s) 289
BstMWI GCNNNNNNNGC 1 cut(s) 107
BstNSI RCATGY 2 cut(s) 222, 328
BstSFI CTRYAG 1 cut(s) 340
BsuI GTATCC 1 cut(s) 575
BsuRI GGCC 1 cut(s) 110
BtsCI GGATG 1 cut(s) 162
BtsIMutI CAGTG 1 cut(s) 109
Csp6I GTAC 4 cut(s) 11, 79, 208, 328
CviAII CATG 5 cut(s) 219, 277, 325, 358, 592
CviJI RGCY 7 cut(s) 5, 69, 101, 110, 273, 524, 540
CviKI_1 RGCY 7 cut(s) 5, 69, 101, 110, 273, 524, 540
CviQI GTAC 4 cut(s) 11, 79, 208, 328
DdeI CTNAG 1 cut(s) 6
DpnI GATC 1 cut(s) 578
DpnII GATC 1 cut(s) 576
EaeI YGGCCR 1 cut(s) 108
Eco57I CTGAAG 1 cut(s) 501
Eco88I CYCGRG 1 cut(s) 600
FaeI CATG 5 cut(s) 222, 280, 328, 361, 595
FalI AAGNNNNNCTT 2 cut(s) 53, 85
FaqI GGGAC 1 cut(s) 380
FatI CATG 5 cut(s) 218, 276, 324, 357, 591
FauI CCCGC 1 cut(s) 478
Fnu4HI GCNGC 1 cut(s) 108
FokI GGATG 1 cut(s) 149
Fsp4HI GCNGC 1 cut(s) 108
FspBI CTAG 1 cut(s) 98
GluI GCNGC 1 cut(s) 108
GsaI CCCAGC 1 cut(s) 528
HaeIII GGCC 1 cut(s) 110
Hin1II CATG 5 cut(s) 222, 280, 328, 361, 595
HinfI GANTC 1 cut(s) 306
Hpy166II GTNNAC 5 cut(s) 88, 264, 406, 497, 549
Hpy188I TCNGA 5 cut(s) 22, 481, 521, 561, 581
Hpy8I GTNNAC 5 cut(s) 88, 264, 406, 497, 549
HpyAV CCTTC 2 cut(s) 51, 134
HpyCH4III ACNGT 1 cut(s) 142
HpyCH4IV ACGT 1 cut(s) 314
HpyCH4V TGCA 3 cut(s) 342, 450, 463
HpyF10VI GCNNNNNNNGC 1 cut(s) 107
HpyF3I CTNAG 1 cut(s) 6
HpySE526I ACGT 1 cut(s) 314
Hsp92II CATG 5 cut(s) 222, 280, 328, 361, 595
Kzo9I GATC 1 cut(s) 576
LmnI GCTCC 1 cut(s) 521
LpnPI CCDG 5 cut(s) 89, 115, 148, 224, 510
LweI GCATC 1 cut(s) 585
MaeI CTAG 1 cut(s) 98
MaeII ACGT 1 cut(s) 314
MaeIII GTNAC 2 cut(s) 344, 417
MalI GATC 1 cut(s) 578
MboI GATC 1 cut(s) 576
MboII GAAGA 3 cut(s) 9, 249, 385
MhlI GDGCHC 1 cut(s) 241
MluCI AATT 2 cut(s) 376, 508
MmeI TCCRAC 2 cut(s) 201, 584
MnlI CCTC 8 cut(s) 57, 125, 155, 170, 346, 525, 566, 609
MseI TTAA 1 cut(s) 59
MwoI GCNNNNNNNGC 1 cut(s) 107
NdeII GATC 1 cut(s) 576
NlaIII CATG 5 cut(s) 222, 280, 328, 361, 595
NmeAIII GCCGAG 1 cut(s) 376
NmuCI GTSAC 1 cut(s) 417
NspI RCATGY 2 cut(s) 222, 328
PciI ACATGT 1 cut(s) 218
PfeI GAWTC 1 cut(s) 306
PkrI GCNGC 1 cut(s) 109
PscI ACATGT 1 cut(s) 218
PsiI TTATAA 1 cut(s) 381
PspFI CCCAGC 1 cut(s) 524
PstI CTGCAG 1 cut(s) 344
RsaI GTAC 4 cut(s) 12, 80, 209, 329
RsaNI GTAC 4 cut(s) 11, 79, 208, 328
SaqAI TTAA 1 cut(s) 59
SatI GCNGC 1 cut(s) 108
Sau3AI GATC 1 cut(s) 576
SduI GDGCHC 1 cut(s) 241
SfaNI GCATC 1 cut(s) 585
SfcI CTRYAG 1 cut(s) 340
Sse9I AATT 2 cut(s) 376, 508
SsiI CCGC 2 cut(s) 107, 485
SspMI CTAG 1 cut(s) 98
TaaI ACNGT 1 cut(s) 142
TaiI ACGT 1 cut(s) 317
TaqI TCGA 1 cut(s) 536
TaqII GACCGA 1 cut(s) 303
TasI AATT 2 cut(s) 376, 508
TatI WGTACW 1 cut(s) 327
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 1 cut(s) 306
Tru1I TTAA 1 cut(s) 59
Tru9I TTAA 1 cut(s) 59
TscAI CASTG 1 cut(s) 109
TseFI GTSAC 1 cut(s) 417
Tsp45I GTSAC 1 cut(s) 417
TspDTI ATGAA 2 cut(s) 265, 521
TspRI CASTG 1 cut(s) 109
XapI RAATTY 1 cut(s) 508
XceI RCATGY 2 cut(s) 222, 328
XspI CTAG 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.