MD12G1225200.v1.1

Protein of unknown function (DUF679)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001834
Physical Location & Seq
Reverse (-)
30085890 .. 30086297
408 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1225200.v1.1.491

Sequence Viewer

Length: 408 bp
ATGGACAACTACCGAAAAATCTACTATGGAATCGCTACATTCAAAGGCATGTACATTTTCAACTGCAGTAACACCGAGGACATGGATAGGGACTTGAAGAAGCATAGAATAAAGTTCATAGATTTTGTTCACGCCTTTATGTCACTGCTTGTGTTCTTGGTCTTTGAACTCAGCAACTCCAGTGTGCAAAGCTCCTTTTCTGAACTGGGAATCAGTGAACTAACCATGAATTTGCCACTCGGAGCTGGGAATTTGTCGAGTTTCTTGTTCACAATCTTTCCGACTACTTGTAGAGGGATCGGATGCGGAGATATGGTTCCCAGGCATTGTAGTGTTAGTAACAGTAATTACATGCACATTTTGTTGAGAGTCGGTGATGTGAGGACAATTTGTCCCACACATCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF679

Protein Analysis

136

Amino Acids

15.36

Weight (kDa)

8.32

Isoelectric Point (pI)

38.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 4 - 102 6.8e-26 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 306
AclWI GGATC 1 cut(s) 305
AcsI RAATTY 2 cut(s) 229, 250
AfaI GTAC 1 cut(s) 53
AfiI CCNNNNNNNGG 1 cut(s) 402
AgsI TTSAA 4 cut(s) 43, 61, 97, 167
AhdI GACNNNNNGTC 1 cut(s) 390
AjnI CCWGG 1 cut(s) 320
AleI CACNNNNGTG 1 cut(s) 403
AloI GAACNNNNNNTCC 2 cut(s) 300, 332
AluBI AGCT 2 cut(s) 192, 245
AluI AGCT 2 cut(s) 192, 245
AlwI GGATC 1 cut(s) 305
ApoI RAATTY 2 cut(s) 229, 250
AsuHPI GGTGA 1 cut(s) 386
BciT130I CCWGG 1 cut(s) 322
BfmI CTRYAG 1 cut(s) 64
Bme1390I CCNGG 1 cut(s) 322
BmeRI GACNNNNNGTC 1 cut(s) 390
BmiI GGNNCC 1 cut(s) 318
BmrFI CCNGG 1 cut(s) 322
BmrI ACTGGG 1 cut(s) 215
BmsI GCATC 1 cut(s) 293
BmuI ACTGGG 1 cut(s) 215
BpmI CTGGAG 1 cut(s) 163
BsaJI CCNNGG 2 cut(s) 75, 320
BsaXI ACNNNNNCTCC 2 cut(s) 300, 330
Bsc4I CCNNNNNNNGG 1 cut(s) 402
Bse1I ACTGG 2 cut(s) 180, 210
BseBI CCWGG 1 cut(s) 322
BseDI CCNNGG 2 cut(s) 75, 320
BseGI GGATG 1 cut(s) 308
BseLI CCNNNNNNNGG 1 cut(s) 402
BseMII CTCAG 1 cut(s) 184
BseNI ACTGG 2 cut(s) 180, 210
BseYI CCCAGC 1 cut(s) 245
BslFI GGGAC 2 cut(s) 104, 378
BslI CCNNNNNNNGG 1 cut(s) 402
BsmFI GGGAC 2 cut(s) 104, 378
Bsp1407I TGTACA 1 cut(s) 51
Bsp143I GATC 1 cut(s) 297
BspACI CCGC 1 cut(s) 306
BspCNI CTCAG 1 cut(s) 183
BspLI GGNNCC 1 cut(s) 318
BspMAI CTGCAG 1 cut(s) 68
BspPI GGATC 1 cut(s) 305
BsrGI TGTACA 1 cut(s) 51
BsrI ACTGG 2 cut(s) 180, 210
BssECI CCNNGG 2 cut(s) 75, 320
BssMI GATC 1 cut(s) 297
Bst2UI CCWGG 1 cut(s) 322
Bst4CI ACNGT 1 cut(s) 344
BstAUI TGTACA 1 cut(s) 51
BstDEI CTNAG 1 cut(s) 170
BstF5I GGATG 1 cut(s) 308
BstKTI GATC 1 cut(s) 300
BstMBI GATC 1 cut(s) 297
BstNI CCWGG 1 cut(s) 322
BstNSI RCATGY 2 cut(s) 52, 355
BstSCI CCNGG 1 cut(s) 320
BstSFI CTRYAG 1 cut(s) 64
BtsCI GGATG 1 cut(s) 308
BtsI GCAGTG 1 cut(s) 143
BtsIMutI CAGTG 3 cut(s) 143, 187, 220
Csp6I GTAC 1 cut(s) 52
CviAII CATG 4 cut(s) 49, 82, 226, 352
CviJI RGCY 2 cut(s) 192, 245
CviKI_1 RGCY 2 cut(s) 192, 245
CviQI GTAC 1 cut(s) 52
DdeI CTNAG 1 cut(s) 170
DpnI GATC 1 cut(s) 299
DpnII GATC 1 cut(s) 297
DriI GACNNNNNGTC 1 cut(s) 390
Eam1105I GACNNNNNGTC 1 cut(s) 390
EcoRII CCWGG 1 cut(s) 320
FaeI CATG 4 cut(s) 52, 85, 229, 355
FaiI YATR 9 cut(s) 27, 50, 83, 105, 119, 140, 227, 314, 353
FaqI GGGAC 2 cut(s) 104, 378
FatI CATG 4 cut(s) 48, 81, 225, 351
FokI GGATG 1 cut(s) 315
GsaI CCCAGC 1 cut(s) 249
GsuI CTGGAG 1 cut(s) 163
Hin1II CATG 4 cut(s) 52, 85, 229, 355
HinfI GANTC 3 cut(s) 30, 210, 369
HphI GGTGA 1 cut(s) 386
Hpy166II GTNNAC 3 cut(s) 130, 218, 270
Hpy188I TCNGA 4 cut(s) 202, 242, 282, 302
Hpy8I GTNNAC 3 cut(s) 130, 218, 270
HpyCH4III ACNGT 1 cut(s) 344
HpyCH4V TGCA 3 cut(s) 66, 187, 355
HpyF3I CTNAG 1 cut(s) 170
Hsp92II CATG 4 cut(s) 52, 85, 229, 355
Kzo9I GATC 1 cut(s) 297
LmnI GCTCC 2 cut(s) 197, 242
LpnPI CCDG 5 cut(s) 191, 193, 231, 307, 334
LweI GCATC 1 cut(s) 293
MaeIII GTNAC 3 cut(s) 68, 141, 338
MalI GATC 1 cut(s) 299
MboI GATC 1 cut(s) 297
MboII GAAGA 1 cut(s) 109
MluCI AATT 4 cut(s) 229, 250, 346, 387
MlyI GAGTC 1 cut(s) 378
MmeI TCCRAC 1 cut(s) 305
MnlI CCTC 3 cut(s) 70, 287, 375
MslI CAYNNNNRTG 2 cut(s) 330, 403
MspR9I CCNGG 1 cut(s) 322
MvaI CCWGG 1 cut(s) 322
NdeII GATC 1 cut(s) 297
NlaIII CATG 4 cut(s) 52, 85, 229, 355
NlaIV GGNNCC 1 cut(s) 318
NmuCI GTSAC 1 cut(s) 141
NspI RCATGY 2 cut(s) 52, 355
OliI CACNNNNGTG 1 cut(s) 403
PfeI GAWTC 2 cut(s) 30, 210
PleI GAGTC 1 cut(s) 377
PpsI GAGTC 1 cut(s) 377
Psp6I CCWGG 1 cut(s) 320
PspFI CCCAGC 1 cut(s) 245
PspGI CCWGG 1 cut(s) 320
PspN4I GGNNCC 1 cut(s) 318
PstI CTGCAG 1 cut(s) 68
RsaI GTAC 1 cut(s) 53
RsaNI GTAC 1 cut(s) 52
RseI CAYNNNNRTG 2 cut(s) 330, 403
Sau3AI GATC 1 cut(s) 297
SchI GAGTC 1 cut(s) 378
ScrFI CCNGG 1 cut(s) 322
SetI ASST 2 cut(s) 194, 247
SfaNI GCATC 1 cut(s) 293
SfcI CTRYAG 1 cut(s) 64
SmiMI CAYNNNNRTG 2 cut(s) 330, 403
Sse9I AATT 4 cut(s) 229, 250, 346, 387
SsiI CCGC 1 cut(s) 306
StyD4I CCNGG 1 cut(s) 320
TaaI ACNGT 1 cut(s) 344
TaqI TCGA 1 cut(s) 257
TasI AATT 4 cut(s) 229, 250, 346, 387
TatI WGTACW 1 cut(s) 51
TfiI GAWTC 2 cut(s) 30, 210
TscAI CASTG 3 cut(s) 150, 187, 220
TseFI GTSAC 1 cut(s) 141
Tsp45I GTSAC 1 cut(s) 141
TspDTI ATGAA 2 cut(s) 106, 242
TspRI CASTG 3 cut(s) 150, 187, 220
XapI RAATTY 2 cut(s) 229, 250
XceI RCATGY 2 cut(s) 52, 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.